LOCUS       DACCCR010000001       102779 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCCR010000001 DACCCR010000000
VERSION     DACCCR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 102779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.001"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVK93_000001"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVK93_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6385326.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IVK93_000002"
     CDS             1995..4661
                     /locus_tag="IVK93_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6385327.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IVK93_000003"
     CDS             5025..5777
                     /locus_tag="IVK93_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6385328.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IVK93_000004"
     CDS             complement(5799..6953)
                     /locus_tag="IVK93_000004"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP6385329.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IVK93_000005"
     CDS             7138..9579
                     /locus_tag="IVK93_000005"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP6385330.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IVK93_000006"
     CDS             complement(9909..10613)
                     /locus_tag="IVK93_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385331.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IVK93_000007"
     CDS             complement(10800..11045)
                     /locus_tag="IVK93_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385332.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IVK93_000008"
     CDS             complement(11062..11913)
                     /locus_tag="IVK93_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6385333.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IVK93_000009"
     CDS             12079..13083
                     /locus_tag="IVK93_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP6385334.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13324..13680)
                     /locus_tag="IVK93_000010"
     CDS             complement(13324..13680)
                     /locus_tag="IVK93_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6385335.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14048..15235
                     /gene="tuf"
                     /locus_tag="IVK93_000011"
     CDS             14048..15235
                     /gene="tuf"
                     /locus_tag="IVK93_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6385336.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IVK93_000012"
     CDS             complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IVK93_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6385337.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18040..18759)
                     /locus_tag="IVK93_000013"
     CDS             complement(18040..18759)
                     /locus_tag="IVK93_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6385338.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            18968..19600
                     /locus_tag="IVK93_000014"
     CDS             18968..19600
                     /locus_tag="IVK93_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP6385339.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            19659..20519
                     /locus_tag="IVK93_000015"
     CDS             19659..20519
                     /locus_tag="IVK93_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP6385340.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20626..23019
                     /locus_tag="IVK93_000016"
     CDS             20626..23019
                     /locus_tag="IVK93_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6385341.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23201..25042
                     /locus_tag="IVK93_000017"
     CDS             23201..25042
                     /locus_tag="IVK93_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP6385342.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            25066..26175
                     /gene="rpoD"
                     /locus_tag="IVK93_000018"
     CDS             25066..26175
                     /gene="rpoD"
                     /locus_tag="IVK93_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP6385343.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            26395..28494
                     /locus_tag="IVK93_000019"
     CDS             26395..28494
                     /locus_tag="IVK93_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6385344.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            28690..29553
                     /locus_tag="IVK93_000020"
     CDS             28690..29553
                     /locus_tag="IVK93_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6385345.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            29658..30131
                     /locus_tag="IVK93_000021"
     CDS             29658..30131
                     /locus_tag="IVK93_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6385346.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            30227..31114
                     /gene="xerD"
                     /locus_tag="IVK93_000022"
     CDS             30227..31114
                     /gene="xerD"
                     /locus_tag="IVK93_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP6385347.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            31133..31504
                     /locus_tag="IVK93_000023"
     CDS             31133..31504
                     /locus_tag="IVK93_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6385348.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            31474..32271
                     /locus_tag="IVK93_000024"
     CDS             31474..32271
                     /locus_tag="IVK93_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP6385349.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            32255..32824
                     /gene="scpB"
                     /locus_tag="IVK93_000025"
     CDS             32255..32824
                     /gene="scpB"
                     /locus_tag="IVK93_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP6385350.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            32830..33546
                     /locus_tag="IVK93_000026"
     CDS             32830..33546
                     /locus_tag="IVK93_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6385351.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      33628..33753
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            33876..34487
                     /locus_tag="IVK93_000027"
     CDS             33876..34487
                     /locus_tag="IVK93_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6385352.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            34865..36388
                     /locus_tag="IVK93_000028"
     CDS             34865..36388
                     /locus_tag="IVK93_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP6385353.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            36625..37965
                     /locus_tag="IVK93_000029"
     CDS             36625..37965
                     /locus_tag="IVK93_000029"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP6385354.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            38118..38855
                     /locus_tag="IVK93_000030"
     CDS             38118..38855
                     /locus_tag="IVK93_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP6385355.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(39020..39241)
                     /locus_tag="IVK93_000031"
     CDS             complement(39020..39241)
                     /locus_tag="IVK93_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP6385356.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            39272..40303
                     /locus_tag="IVK93_000032"
     CDS             39272..40303
                     /locus_tag="IVK93_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385357.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            40290..41693
                     /locus_tag="IVK93_000033"
     CDS             40290..41693
                     /locus_tag="IVK93_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP6385358.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            41759..42394
                     /locus_tag="IVK93_000034"
     CDS             41759..42394
                     /locus_tag="IVK93_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6385359.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            42443..43123
                     /locus_tag="IVK93_000035"
     CDS             42443..43123
                     /locus_tag="IVK93_000035"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP6385360.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            43258..44487
                     /gene="rpsA"
                     /locus_tag="IVK93_000036"
     CDS             43258..44487
                     /gene="rpsA"
                     /locus_tag="IVK93_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP6385361.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            44669..45979
                     /locus_tag="IVK93_000037"
     CDS             44669..45979
                     /locus_tag="IVK93_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP6385362.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            46222..46497
                     /locus_tag="IVK93_000038"
     CDS             46222..46497
                     /locus_tag="IVK93_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP6385363.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            46732..47778
                     /locus_tag="IVK93_000039"
     CDS             46732..47778
                     /locus_tag="IVK93_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385364.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            47780..49039
                     /locus_tag="IVK93_000040"
     CDS             47780..49039
                     /locus_tag="IVK93_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6385365.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            49045..49626
                     /locus_tag="IVK93_000041"
     CDS             49045..49626
                     /locus_tag="IVK93_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP6385366.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(49708..50583)
                     /locus_tag="IVK93_000042"
     CDS             complement(49708..50583)
                     /locus_tag="IVK93_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6385367.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            50731..51063
                     /locus_tag="IVK93_000043"
     CDS             50731..51063
                     /locus_tag="IVK93_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6385368.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            51175..52383
                     /locus_tag="IVK93_000044"
     CDS             51175..52383
                     /locus_tag="IVK93_000044"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP6385369.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            52537..52992
                     /locus_tag="IVK93_000045"
     CDS             52537..52992
                     /locus_tag="IVK93_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385370.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            53354..53629
                     /locus_tag="IVK93_000046"
     CDS             53354..53629
                     /locus_tag="IVK93_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385371.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            53642..55720
                     /locus_tag="IVK93_000047"
     CDS             53642..55720
                     /locus_tag="IVK93_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6385372.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            55880..57766
                     /locus_tag="IVK93_000048"
     CDS             55880..57766
                     /locus_tag="IVK93_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6385373.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            57778..58725
                     /locus_tag="IVK93_000049"
     CDS             57778..58725
                     /locus_tag="IVK93_000049"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6385374.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            58745..59272
                     /locus_tag="IVK93_000050"
     CDS             58745..59272
                     /locus_tag="IVK93_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6385375.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(59335..59988)
                     /locus_tag="IVK93_000051"
     CDS             complement(59335..59988)
                     /locus_tag="IVK93_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6385376.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            60120..60962
                     /locus_tag="IVK93_000052"
     CDS             60120..60962
                     /locus_tag="IVK93_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6385377.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            61120..62016
                     /locus_tag="IVK93_000053"
     CDS             61120..62016
                     /locus_tag="IVK93_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6385378.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            62019..62732
                     /locus_tag="IVK93_000054"
     CDS             62019..62732
                     /locus_tag="IVK93_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP6385379.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            62752..63270
                     /gene="msrA"
                     /locus_tag="IVK93_000055"
     CDS             62752..63270
                     /gene="msrA"
                     /locus_tag="IVK93_000055"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6385380.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            63267..63494
                     /locus_tag="IVK93_000056"
     CDS             63267..63494
                     /locus_tag="IVK93_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP6385381.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            63646..64197
                     /gene="lepB"
                     /locus_tag="IVK93_000057"
     CDS             63646..64197
                     /gene="lepB"
                     /locus_tag="IVK93_000057"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6385382.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(64280..64822)
                     /locus_tag="IVK93_000058"
     CDS             complement(64280..64822)
                     /locus_tag="IVK93_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6385383.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(65059..65457)
                     /locus_tag="IVK93_000059"
     CDS             complement(65059..65457)
                     /locus_tag="IVK93_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6385384.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            65717..66577
                     /gene="ylqF"
                     /locus_tag="IVK93_000060"
     CDS             65717..66577
                     /gene="ylqF"
                     /locus_tag="IVK93_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP6385385.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            66570..67337
                     /locus_tag="IVK93_000061"
     CDS             66570..67337
                     /locus_tag="IVK93_000061"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP6385386.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            67393..68250
                     /gene="dprA"
                     /locus_tag="IVK93_000062"
     CDS             67393..68250
                     /gene="dprA"
                     /locus_tag="IVK93_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP6385387.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            68372..70450
                     /gene="topA"
                     /locus_tag="IVK93_000063"
     CDS             68372..70450
                     /gene="topA"
                     /locus_tag="IVK93_000063"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP6385388.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            70419..71795
                     /gene="trmFO"
                     /locus_tag="IVK93_000064"
     CDS             70419..71795
                     /gene="trmFO"
                     /locus_tag="IVK93_000064"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP6385389.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            71993..72898
                     /gene="xerC"
                     /locus_tag="IVK93_000065"
     CDS             71993..72898
                     /gene="xerC"
                     /locus_tag="IVK93_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP6385390.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            72935..73483
                     /gene="hslV"
                     /locus_tag="IVK93_000066"
     CDS             72935..73483
                     /gene="hslV"
                     /locus_tag="IVK93_000066"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP6385391.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            73497..74897
                     /gene="hslU"
                     /locus_tag="IVK93_000067"
     CDS             73497..74897
                     /gene="hslU"
                     /locus_tag="IVK93_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP6385392.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            74915..75706
                     /gene="codY"
                     /locus_tag="IVK93_000068"
     CDS             74915..75706
                     /gene="codY"
                     /locus_tag="IVK93_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP6385393.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            75788..76663
                     /locus_tag="IVK93_000069"
     CDS             75788..76663
                     /locus_tag="IVK93_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP6385394.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(76795..77433)
                     /gene="plsY"
                     /locus_tag="IVK93_000070"
     CDS             complement(76795..77433)
                     /gene="plsY"
                     /locus_tag="IVK93_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP6385395.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            77560..77994
                     /locus_tag="IVK93_000071"
     CDS             77560..77994
                     /locus_tag="IVK93_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP6385396.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            78160..80205
                     /gene="parE"
                     /locus_tag="IVK93_000072"
     CDS             78160..80205
                     /gene="parE"
                     /locus_tag="IVK93_000072"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP6385397.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            80217..82667
                     /gene="parC"
                     /locus_tag="IVK93_000073"
     CDS             80217..82667
                     /gene="parC"
                     /locus_tag="IVK93_000073"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP6385398.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            82944..85175
                     /gene="pflB"
                     /locus_tag="IVK93_000074"
     CDS             82944..85175
                     /gene="pflB"
                     /locus_tag="IVK93_000074"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP6385399.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            85419..86180
                     /gene="pflA"
                     /locus_tag="IVK93_000075"
     CDS             85419..86180
                     /gene="pflA"
                     /locus_tag="IVK93_000075"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP6385400.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            86265..87191
                     /locus_tag="IVK93_000076"
     CDS             86265..87191
                     /locus_tag="IVK93_000076"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP6385401.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            87373..88266
                     /locus_tag="IVK93_000077"
     CDS             87373..88266
                     /locus_tag="IVK93_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP6385402.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            88400..88525
                     /locus_tag="IVK93_000078"
     CDS             88400..88525
                     /locus_tag="IVK93_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6385403.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(88659..89291)
                     /locus_tag="IVK93_000079"
     CDS             complement(88659..89291)
                     /locus_tag="IVK93_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385404.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(89439..89981)
                     /locus_tag="IVK93_000080"
     CDS             complement(89439..89981)
                     /locus_tag="IVK93_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP6385405.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            90116..90505
                     /locus_tag="IVK93_000081"
     CDS             90116..90505
                     /locus_tag="IVK93_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385406.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            90509..91273
                     /locus_tag="IVK93_000082"
     CDS             90509..91273
                     /locus_tag="IVK93_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6385407.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            91402..92349
                     /locus_tag="IVK93_000083"
     CDS             91402..92349
                     /locus_tag="IVK93_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6385408.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            92479..93219
                     /locus_tag="IVK93_000084"
     CDS             92479..93219
                     /locus_tag="IVK93_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385409.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(93510..94178)
                     /locus_tag="IVK93_000085"
     CDS             complement(93510..94178)
                     /locus_tag="IVK93_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6385410.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            94312..94599
                     /locus_tag="IVK93_000086"
     CDS             94312..94599
                     /locus_tag="IVK93_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385411.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            95015..96553
                     /locus_tag="IVK93_000087"
     CDS             95015..96553
                     /locus_tag="IVK93_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP6385412.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            96550..97515
                     /locus_tag="IVK93_000088"
     CDS             96550..97515
                     /locus_tag="IVK93_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6385413.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            97502..98824
                     /locus_tag="IVK93_000089"
     CDS             97502..98824
                     /locus_tag="IVK93_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6385414.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      98887..98991
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            99065..99808
                     /locus_tag="IVK93_000090"
     CDS             99065..99808
                     /locus_tag="IVK93_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6385415.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            99823..100815
                     /locus_tag="IVK93_000091"
     CDS             99823..100815
                     /locus_tag="IVK93_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6385416.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            100827..101564
                     /locus_tag="IVK93_000092"
     CDS             100827..101564
                     /locus_tag="IVK93_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6385417.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            101554..102276
                     /locus_tag="IVK93_000093"
     CDS             101554..102276
                     /locus_tag="IVK93_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6385418.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(102360..>102779)
                     /locus_tag="IVK93_000094"
     CDS             complement(102360..>102779)
                     /locus_tag="IVK93_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6385419.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        34882 a        17714 c        21591 g        28592 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
    13201 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    13261 ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat
    13321 ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc
    13381 ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa
    13441 tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc
    13501 cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat
    13561 ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc
    13621 tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat
    13681 gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt
    13741 ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta
    13801 aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt
    13861 tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac gtataataag
    13921 cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa
    13981 gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt
    14041 gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat
    14101 tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa
    14161 aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga
    14221 acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc
    14281 gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca
    14341 aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga
    14401 acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt
    14461 tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt
    14521 gagcgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc
    14581 attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga
    14641 atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt
    14701 cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg
    14761 tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac
    14821 aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt
    14881 gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg
    14941 ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg
    15001 tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga
    15061 tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt
    15121 gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat
    15181 tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac
    15241 gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat
    15301 ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac
    15361 tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt
    15421 ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca
    15481 actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta
    15541 gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat
    15601 ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt
    15661 aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat
    15721 tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca
    15781 gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg
    15841 taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca
    15901 ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt
    15961 cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat
    16021 ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga
    16081 gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc
    16141 cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt
    16201 tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct
    16261 tctggaaaat cttctttctt accagaagga tcacccatta caatacattt attattgaag
    16321 caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta
    16381 tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg
    16441 gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca
    16501 aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt
    16561 tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atctggcaga
    16621 ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc
    16681 gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga
    16741 atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa
    16801 acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt
    16861 gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg
    16921 ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct
    16981 tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg
    17041 attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa
    17101 gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag
    17161 aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac
    17221 aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt
    17281 cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc
    17341 attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact
    17401 agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa
    17461 gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat
    17521 tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa
    17581 atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg
    17641 ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa
    17701 ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc
    17761 tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg taacacagag
    17821 ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct
    17881 cctaattgct ggatagatag agttttaccg atggacatca attcaccgac aataatgaca
    17941 atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg
    18001 gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg
    18061 aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt
    18121 cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt
    18181 gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt
    18241 ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact
    18301 tgggcgaaat aaagagagta accataccca tgcataatga atattactga tgactctggc
    18361 agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt
    18421 tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa
    18481 atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg
    18541 aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata
    18601 cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc
    18661 attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct
    18721 cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag
    18781 tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg
    18841 taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg
    18901 aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg
    18961 tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct
    19021 gacggttatt ggtgcggtgt tgtttttagg aggaagaact tatcgttcgg taaaacgagt
    19081 ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga
    19141 gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca
    19201 gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga
    19261 tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt
    19321 atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca
    19381 tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact
    19441 tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa
    19501 tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa
    19561 tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat
    19621 catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg
    19681 aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt
    19741 ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg
    19801 gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg
    19861 ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt
    19921 tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta
    19981 tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg
    20041 atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta tacggcgctc
    20101 aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga
    20161 ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat
    20221 tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg
    20281 gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa
    20341 aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca acgattatga
    20401 tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt
    20461 ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa
    20521 gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt
    20581 tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg
    20641 gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag
    20701 aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat
    20761 gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg
    20821 ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc
    20881 aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata
    20941 catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc
    21001 cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa
    21061 atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca
    21121 aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg
    21181 ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca
    21241 gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa
    21301 cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg
    21361 atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt
    21421 gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt
    21481 gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat
    21541 tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact
    21601 gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc
    21661 ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct
    21721 atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct
    21781 tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg
    21841 cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat
    21901 ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca
    21961 gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa
    22021 gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat
    22081 ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc
    22141 gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt
    22201 tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc
    22261 agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat
    22321 tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg
    22381 ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac
    22441 aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg
    22501 attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt
    22561 gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa
    22621 atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta
    22681 gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa
    22741 actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct
    22801 tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat
    22861 gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta
    22921 ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct
    22981 gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt
    23041 gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta
    23101 aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct
    23161 gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca
    23221 agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt
    23281 ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac
    23341 accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg
    23401 cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt
    23461 aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc
    23521 tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga
    23581 aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca
    23641 aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga
    23701 gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg
    23761 ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt
    23821 gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc
    23881 acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact
    23941 gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa
    24001 cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat
    24061 caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga
    24121 aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac
    24181 caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt
    24241 tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga
    24301 attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg
    24361 gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact
    24421 tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt
    24481 tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca
    24541 aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg
    24601 gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt
    24661 taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc
    24721 atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct
    24781 tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga
    24841 atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat
    24901 cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag
    24961 acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa
    25021 acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca
    25081 gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga
    25141 caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat
    25201 gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat
    25261 ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca
    25321 gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa
    25381 gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc
    25441 gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta
    25501 agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga
    25561 aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca
    25621 acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt
    25681 acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt
    25741 caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat
    25801 cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa
    25861 acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca
    25921 acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta
    25981 gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc
    26041 cggacgagaa cattggaaga agtagggaaa gtgtttggtg ttactcggga acgtattcgt
    26101 cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa
    26161 gatttcttag aataagcgtg gactattgac cagaagagaa atcagtaaaa ctgtttggtt
    26221 tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa gctttttttt ctataggaag
    26281 gaaattcatt cattattttt aagtaaatcc taaaaataag ctattggcgt gacgtataga
    26341 atatgataga ttaaatggta gcgtaaacct agtaaaggag ccgatcgcta agtgatgaaa
    26401 aaatgtccaa attgcggatc gaaaaaaatg gagcagaata ctatctgtcc agactgcggt
    26461 catcaaataa ctgcagaaga tcagcaaata aaagaatcaa ttgaagaaac aaatacagat
    26521 gcagctatgc aggataaatc aactgaagat gcagtctttc ctgatacaga attaaacgat
    26581 ccaatcgaat ggtcggaatt gaaagattta cctctagaat ctgtaatgga attattcgaa
    26641 aaagcagaac cagaagacga agaaccacct gctcacgatc aagtaactaa tatagagaaa
    26701 aatgaacaga agaaagcaac tgttcatgag acttcggaag aatcaggaaa gaatcaagaa
    26761 gaaacgcttg aatcaaaaca aaaaagaaga gtagctgaac tgaaagaaat cgtcgataat
    26821 gaggaagaaa attcgatact atccgcttat atcaaagcac atcgtgaaga tacaaaagaa
    26881 gagcacgcga aagagctgct caaaatgatc agtgaaaaaa ttgcacagga aaacaaagta
    26941 gagccagaaa aggaacaaga aactgaaaaa gactctgata gagaaaacgc cacaggtaaa
    27001 gaaacagtaa aaataaaaga cgtaaaaacg gatatcacag aaaaaactga agtatttact
    27061 gaaaaacaat tagaaaagga aaaaacgaat aaactgccgg aagcagagaa aaaagaaata
    27121 ctcgaaaaca cagacaaaaa gtcaacagag cccgatgaaa taaatagtga atccgatgct
    27181 gaaaatcaat ctgtaagcaa agagggtact gatcttgaag caaaaataga aaaagaagag
    27241 aaccagccta agctgtttga agcagaagcg aaatcaataa caatcgtgcc agatactgaa
    27301 aaaacacatc aagaatcaaa cttagaaaaa acagatccag ataaagcgca aaagaaaaaa
    27361 tcaaaaaaag taccttatct cgttttagca gcagtacttc tactcggtgg aggaagctgg
    27421 gcttattatg accatcatca aaaagtagaa gccgaagtag cagcagaagc aaaaagacag
    27481 caacaaatgg aagaacttca tgagaaactt gctgcctttt atacagatga tcaccatcaa
    27541 tttattcgtg cagatatgat tcatcaagat ttatcaaaac taaaaaatga tttatccagt
    27601 cttaagaatg aaaaaggata ttcagagtta gaaacaactt accaagatat ccaatctaag
    27661 atccgagata tccaaaaagt gaatgagtgg ttcatagcac ctgccatcgt cgatgatcat
    27721 ctagcagatg acatcaagtt gaaagcagat aaagagatcc aagcaatcga aacggataac
    27781 acagattttg gaaaattgat tcaacaagca caaacagaag ctaaaaatca atatgctgaa
    27841 ttgcaaacag ctaaagaaaa aacagctata gtctttaaag acggaaaagt agtggattca
    27901 gcaacacgcg accaatacaa gacggcaaaa gaagcagtag aaaaagtgaa aaatacagat
    27961 ctatctgctc agttgaatga acaattgaaa caagtagata agatgctcac tgataaagag
    28021 aaaaaagaag aagaggaaaa gaaaaaagca gaagaagcga aaaaagcagc tgaagaacaa
    28081 gcaaaacaag ccgctcaagc agcagctgca gctcaagccg ctcaacaagc tgcggtcgca
    28141 caagcagcac agcaagaagc ggcatcctcg accccttcct cttcaaactc agcgaatcag
    28201 ccaatcaaat ctgccagagc aagtgatatt gccgatgcat ctaatccagc atggacgtgg
    28261 gcaccaggtg tccaaagcaa agtgattgct acatgtatcc aacgtggata tatcgttgaa
    28321 ggaggttaca gattagaaaa agccagaatc gaaaatgggg aaggctatta taatctatat
    28381 gccacttcaa caaaatctgc attgatgaaa ggcattggag aaagtgcttt gccattttac
    28441 atcgtaacca tcaactgtaa aactggatgg ttcggaggaa atgggagcaa ttaattttag
    28501 gcaaatgaaa gaggctgtga gcctgccgtt taacaccgtt tattcgaaaa aggggagtat
    28561 gacacaactg ttgtcatact ccttttttca cctttccaaa ccaaggctca gctattcaag
    28621 tagataacat tatgttatga tagcaaagaa atgtattaag aaaacctttc actatagagg
    28681 gagaaaaaaa tgaacgattt attagcaact atatttacag gtattattat tgatgaaaat
    28741 gaaaaaaact actttgtcca aaaaaacgga caaacctttg gcttaagcaa atcagaaggt
    28801 gaacacaaaa tcggtgaagc agttgaagga ttcggttacc aaaatcaaaa acatgaaaat
    28861 cggatcacta cacaaattcc taaaagccgt atcggtcatt acggttttgg aatcgttact
    28921 gctacacgaa aagatttagg tgcgtttgtc gatatcggtc ttccagataa agactttgtc
    28981 gtatctcttg atgaattgcc gacaatgaca gagctatggc ctaaaaaagg ggatcgcttg
    29041 atgatttccc ttagagttga tgcaaaagac cgtatttggg gcagtttagc agatgaaaaa
    29101 atattcaaat ctttaagcaa atatggaaca gaagaaatga aaaacaaaga tgtgatagga
    29161 acagcatacc gcttgaaatt aacaggtacg tacgttttaa cggaagattt ctatcttggg
    29221 tttatccatc cttctgaacg atataatgaa cctcgtctag gcgagcagat aaaaggtcga
    29281 gtgattggtg tccgaccaga cggtgtactg aatctttcat tgaagccaag agcatatgaa
    29341 gctatatcag atgatgcaca aatgatttta accattttag aacgttctgc agatcagcaa
    29401 atcaacttta cagataaatc ggatccagaa gaaatcatga aagcttttgg tatcagcaaa
    29461 ggagcattta agcgcgcatt aggtaatctt ttgaaacaag gattgattac acaagaaaac
    29521 ggtgtgacaa aactagtcaa aaaatctaat tgagaatccc ttgcatgttt ttcttaacta
    29581 gcttataata attttaatta acatagtgtt atatcttttt ttatttgtaa ttattataaa
    29641 ttgttggggt tgagtaaatg gtcatgaatg ctatttcggc gatgaaaaaa acaaaacagc
    29701 agctacatga agcggggttt aagctgacac cgcaaagaga agctactctg cttgtgcttt
    29761 tggaaaatga gaaagagcat ttatctgcag aagaaatctt ctttttagtg aaaaaaaaga
    29821 gtccggaaat tggacttgct actgtttatc gaacccttga aatcttgaca gaattgaaaa
    29881 ttgtcgataa agtaagtttt aatgatggat tagcccgtta tgatttgaga aaagaaggcg
    29941 ccaaacattt ccatcatcat ttactatgct tgaactgcgg cacgatcgag gaagtagaag
    30001 aggatctttt acttgaagtg gagcaagtag tagaagaacg ttatcatttt ttagtaaaag
    30061 atcatcggct aacgttccat ggaatctgtc aggaatgcta tgaaaaagaa aaaagaaacc
    30121 agtccgtctg agtgtctaga cggctttttc tttgctcaga ttatggaaaa agactatcag
    30181 aatgaagcgg tatctgatat gatagagagg catgaggggg attattatga aagagcaaat
    30241 caacgattat ctccactact tgaatattga acggggattg tccgccaata cacgacaaag
    30301 ctatgaacgt gatttggaac agtacttgca ttttctacag gagcagaaaa ttgcttcatg
    30361 gcaggatgtc gatcgctttt tagtcatccg ttttctggag aagttgcatg aagaaaaaaa
    30421 agcgacagca actgttactc ggatgatctc aagtctcaga agattccatc agtttttacg
    30481 gcaagagcgt atcactgatc atgatccgat gcaacatatc gatacgccga agaaagcaca
    30541 gaaattacca agtacattga ctctgacaga agtagaaaag ctgattgaag cacctgatac
    30601 gacaaaaacg ttagggataa gagatcgtgc aatcttggaa gtcatgtatg ctactgggat
    30661 gcgcgtcagt gaattgattg gattgaaatt gggagatctt catctttctc ttggactggt
    30721 acagacagtt ggaaaaggag ataaagagcg gatcattcct ttaggtgatt atgcgatcca
    30781 atggctggaa cgctacttag acgaagcacg tccattactt gtaaaggacg catctgaaat
    30841 gcatgtattt gtcaataatc atgggaaagg tctttctcgg caaggaatct ggaaaaatct
    30901 gaagcagtta gtacgagagg ctgggattta caaagaagta acaccacata cgctgcggca
    30961 tagctttgcc acacatctgc tagaaaatgg agctgatttg cggacagtac aggaattgtt
    31021 aggtcacgcg gatatctcta cgactcagat ttatacacat atcacgaaga aaagaatgac
    31081 ggatgtttat aagcagcatt ttccaagggc ttagtcatag gaaggtgaga agatgttaac
    31141 acattaccgg aaagaaaacc gtaaaattgc tatgggacta ttgagtttcc ataagaaact
    31201 gacaggtaaa acttctttgt tgaaggagat tgatacgtac gaatctgaag acgattatga
    31261 actttttttc tttacgccag aaggttcaac caacattcaa gggattattg gtatttactg
    31321 ggcaacgaac gatcagctga tcatccatga catttctttg aatccgtctt atcgcggtga
    31381 gaatatgggc tttcaaatgt tagatgaatt acaggaaaag tacccggatg tctccatccg
    31441 tgcaactgaa atcacagagc cttacattgg aaaatggcta gcaaagaaaa agaagaaaga
    31501 gtgaatgagt tgacggagat caatattaaa ttagatgtat tcgagggtcc tctcgatctg
    31561 ctgcttcatc tgattcagca aatggaaatc gatatatatg atataccgat cgctacgatc
    31621 acggagcaat atatgaatta tatccataca atgaaaacac tcgagctagc tgttgcagga
    31681 gaatacttag tgatggctgc tacattgatg gcaatcaaaa gcaagacatt gcttcctgtc
    31741 caagaattcg tcacagacga tgaagaatta tgggaagaag atcctcgaga agaattggtc
    31801 actcagcttt tagaataccg taaatataaa tatgcagctg aaagactgac agaaaaagca
    31861 gaggaacgaa gtctttatta cacaaaagaa cctatgaaca tcgatgggtt agcggatgct
    31921 gaaaagccgt tgaagcgagg acaagtcaaa aaaatcgatt tgatccgtgc catgcagaaa
    31981 atcttagaac gtaagaaatt ttctcaaaaa gtggaaaaaa cgatcacgcc cgatgacaca
    32041 acgatcgaag aacgaatggt atttatcgaa gagcgtataa aaagaaatgc gcatggctgt
    32101 actttcgatt ctttttttga gtcaccttct aaaagtgaag ttgtcactac ttttatggct
    32161 ttgctagaat tgatgaaaaa agggcagatc attgcgcaac aaaaagaaaa ctatgaatca
    32221 attatgcttt atccagcagt aggagatacc gaacatgacg aaattaagtg aacttgaagc
    32281 ccttcttttt attgttggcg aggaagggat cagtaatgaa gaattaagcg gactgttaaa
    32341 tctcagcgaa gcagaagtca caagtttagt gaaagaatta aaagaatctt acactcaaaa
    32401 ctcgcactca gctttgcata tttttgaaac ggaaagccat tatgtattaa cgactaaaaa
    32461 agagctggct cccttactta agcgatatgc gcaagggaca tcaaacacat tatctcaagc
    32521 cgccgtcgaa accttagcaa tcatcgctta taaacagcca atcacaagag ctgaaatcga
    32581 gcagattcga ggagttcagg tttcaggagc ggtacagcgt ctagctgccc ataaactgat
    32641 tgaagaaaaa ggtagagtag aaggaccagg tcggccgata ttatatggaa caactggcta
    32701 cttcttagat tattttggtt tgaaatcatt agaagaactg cctgatacac aagaaatgga
    32761 aatttcactt gaagaagacg tgtcaccaga tctattcttt gatggaatga ataaaggaga
    32821 ataattgtaa tggagagatt gcagaaagta attgcccacg ctggcatcac ttctcgtcga
    32881 aaagcagaag aattcatcct tgcaggacgt gtaaaagtaa acggacaagt tgtaagggaa
    32941 ttagggataa aagttgggaa acatgacact gtggaagtgg atgaagtccc aatttatcaa
    33001 gaagaatacg gttactattt attgtacaaa cctaaaggag ttatttctgc tgttgcagat
    33061 gacaaaggaa gaaaagttgt tacggatctt ttgccgatgg taaaagaacg tatctatcct
    33121 gtaggtcgac tggattacga tacttcgggg atcttgctac tgacaaatga tggagatttt
    33181 gctcagcgcc tgacacatcc aaaacatgaa gtggataaag tttacgttgc aaaggtaaaa
    33241 gggatcgcta caaaatcgat gcttgcacct ttgacaaaag gaataaagat cgaaggacga
    33301 aaaacatctc cagctaaatt ccaaatttta tcagtggatc ctaagacaaa ccacagtatc
    33361 gtggaattga cgatccacga aggacgtaat catcaagtga aaaatatgct tcaagcagtt
    33421 ggtcttcctg tgcaaaaact aaaacgtgaa aaatacggag atctgacttt acaaggccta
    33481 cgtcctgggg attaccgtga actgaacaaa aaagaagtca gcagtttgct gaaccactca
    33541 aaatgaacac ttacttttta taagtgttag atgttgcgtt ttttgaatag atatgtataa
    33601 tgaatatgta aacaaaaatt atataaggtt cgtcttcagg ggcagggtga aattcccgac
    33661 cggtggttat agtccacgac ctattttgca aaaaaatagc tgaatcggtg aaattccgat
    33721 accgacagta tagtctggat aaagaagata gagcttattt gacgacgctt gatcaaataa
    33781 ctctaactct attttccctg gaaaatagag ttttttcatt tggagagaaa cgtcaaaaag
    33841 ttcgctgaag aggagtccta acaggaggat ttcaaatgaa gaacacccgt gtacagaaaa
    33901 tggttgcagt agcgatgttt gctgcgatcg cattagtttt acagtatttt gcttttccga
    33961 tcatgccggc ttttagtttt ttgaagatcg attttagtga tatctcggta ttgatcagca
    34021 tgttcttatt cggtccagcg gccggagttg caacagcgtt cttgcgttca gtattacatc
    34081 tgctgactac aggtttttct ccggataatt tggtaggaga cactgctagt ttcttatcaa
    34141 caacgatctt tacattcccg atttattatt tctttagtgt aaagaaacag cataaaggaa
    34201 gaaacaaatt tttaggtgtc attacaggaa ccatcgctct aactattttt atgagtatcg
    34261 caaattattt cgttattacg cctctttact taatgttttt agggctgaac gcaacacaga
    34321 tgctgggcat gtctttagca aactatgtac tgattgggat cgttcctttc aacttgatca
    34381 aaggattcat cgtgagtgct gtctttttag tgctgcatgc gcaacttctt ccatggttaa
    34441 gtcgaaaaca acatcagttc gaacaacgtc atacagcagt caaataaata tcaaaaagat
    34501 caagaaattt cttggtcttt ttttttggaa aaaaatacaa gacatgcaat aatgtaaacg
    34561 ttttatttgt ttgttttgag tatttttttg atttatgcat aaaaatcaat caattctttg
    34621 caaatgtgaa attcttctcc ttttaaaaga cgaaaatgtt tgtttataaa aaaaaggaaa
    34681 cgctacaatg aaaaagaaga aagctttaaa aacctgaaaa aagaggaggg aatagggaaa
    34741 aacgaacttt ttgaacaaaa gttttacaaa atgcgtgtta ttatttttgt tctgatactc
    34801 tttttgataa aataacatta gagccaaaat tgttgtctct aacggaaaag aaaggaagaa
    34861 gaagatggaa caaacaaaaa agaaaaaatt tcaaatgcct tcagcgtata cggtcttatt
    34921 tattattatt gcagtaatcg ctgtacttac ttggtttata ccagctgggc actatagtgt
    34981 agacgatgca ggcaacatcg tcgctggatc atatgaacga gtacaacaaa atcctcaagg
    35041 gctgtgggat attttcatgg cgccaatcaa tgggatgcta ggtgtaccag cgggggagct
    35101 aactaaagaa acacctgctg caatcccaat ctctttcttc attttggttg ttggtggatt
    35161 tttaggtatt atcaacaaaa caggggcact agatgccggg attgcttcag ttgtgaagaa
    35221 attcaaagga aaagaaaaaa tgcttattcc agtattgatg ctgttatttg cattaggcgg
    35281 ctctacttac ggaatggcag aagaaacgat tccattctat gctttactga tcccagtcat
    35341 gatggctgta ggctttgata cagtagtagc tgtagcaatc gtattagtag ggtctcaagt
    35401 aggctgtcta gcttcaacgg taaatccttt tgcaacaggc gtagcttcac aagctgttgg
    35461 gatttcgatg ggtgaaggta ttggtcttcg attcttgatg ttcgttattt tgttagctat
    35521 ttctattgta tatgtgtatc gttatgcatc aaagatcgaa aaagatccaa caaaatcatt
    35581 agtatacaac caacgtaaag aagatatgaa gcatttcgat attgaagcaa tcggtcgtca
    35641 agaagtaatc acgaaaaaac aaaaacatgt taatgtattg ttctttatta cttttggtat
    35701 catgatcatc agtttgattc cttggacaac cttgaatgcg aatttcacga tttttgaatc
    35761 attgacaaac tggttaacaa accttccggt actaggtacg gttctaggta aaagcatgct
    35821 tccattaggt gactggtact tcccagaaat tactatgtta ttcttagtaa tgtccatcgt
    35881 agtagcaatc acttatggca tgaaagagtc tgaatttgtc tctgaatttt tagctggtgc
    35941 ttctgatttg atcggtgtag caatcgtagt agctgttgct cgtggtatcc aagtggttat
    36001 gaacaatgga ttaatcacag acaccatctt acattggggc gaacaaggat taagttcatt
    36061 atcttcaagt gtattcatca ttattacatt tattttctat atccctatgt cattcttgat
    36121 tccttcaact tccggcttag ctgctgcaac aatgggaatc atgggaccta tgggtgaatt
    36181 tgccggcgtc ggtaaggatt tagtaatcac tgcttatcaa tctgcatctg gtttagtaaa
    36241 cttaattaca ccaacatctg ctatcgtaat gggtgctgta gcaattgctc gtttcgatgt
    36301 ttcagtgtgg tggaaattta ccggtaaatt aattgcgata ttatttgtag ccatctgtgt
    36361 gatcctaggt gtagcagcaa tgttctaaac ctagacaaat acaggtacaa ggtctatcat
    36421 aagaatgaag aaaccgataa acggctatcg ataagcacgt ggataagtca atagctccat
    36481 tcagtgagtg ttttttcttt catcatttgt taagaacatt ctgcttgctg atggagcagg
    36541 cttaaaacgt cattatttga gaagaagagg ttgtgtcgga ggaatttgac atgacccttt
    36601 ttaacatatt aggaggataa tatcatgaaa aaatttgtga cagaacaaca tcatgaacaa
    36661 gcattagaat cattgagcga attgatccga attccttccg tactagatga agcagattca
    36721 ggacaaggac atcccttcgg taaaaaagtg attgaagctt tggataaagt attggaaatc
    36781 agtgaaaagc taggatttag aacatttaaa gatccagaag gctattacgg ttattcagag
    36841 attggctctg gagatgaatt attcggtatc ttgtgtcata tggacgttgt tccagcaggg
    36901 gacgaaaata attgggaaac aaaaccattc gatccaacta taaaagacgg ctggttagtt
    36961 ggtcgcggat cccaagatga caaaggtcct tctattgcgg caatgtatgc cgtcaaagct
    37021 ttgattgatg ctggcgtgga attcaagaca cgtattcgtt ttatctttgg tacagatgaa
    37081 gaaaatttat ggcgttgttt agaaaaatat aacgaaaaag aagaaggaat tacgcaagga
    37141 tttgcgccag atgctgaatt tcctttaatt tatgcagaaa aaggcttatt gcaagcttac
    37201 ttgacaggac caggaacgaa cgagttttct gtaaaagctg gaggagcgtt gaatgttgtt
    37261 cctgatgcag ctccttactc tggagaaaag ctttctgaag taaaagaagc attgaaaaaa
    37321 cacgaattcg atttcgaaga acaaggtgaa ggcatcgtcg ttcttggtaa aagtatccac
    37381 gcgaaagatg cagcacaagg agtgaatgct atttctcgtc tggcaattgc tctttcagaa
    37441 gtatttgatt ttggcccaat caatttctta ggaaaattag ttcaagaaaa tgcaactggt
    37501 gaagctgtcg tgggcaaaac agaagatgaa caatccggcg agctaacgat gaactttgca
    37561 agtttggaaa tcacaccaga acaaacaaaa atcggtgtag atatgagaat ccctgttaca
    37621 ttcaagaaag atgatttagt agctaaatta actaaaacag cagaaaaata tgggttgact
    37681 tatgaagaat ttgacttcct tgattcatta tacgtgccac tagacagtga gttagtgaaa
    37741 aatctattgg ggacatatcg cgatattact ggagacatga ctgaaccgtt tgtatcaggc
    37801 ggcgctactt ttgctcgtac gatgaaccag tgtgtggcat tcggtgcgat gttccctgat
    37861 actcctgact ttatgcacca ggcgaatgaa cgatgggaat taagtagtat gtataaagca
    37921 atggaaattt atgcagaagc tgtgtatcgt ttatgtgcta aataaaaata aacatacaaa
    37981 aagggcggat attcaaatgg atattccgtc tttttttatt tgatgctgga aagtttgcat
    38041 ttctctctta tttttttaag gtataatcat acttgattaa gaaaaagggg tcactcagat
    38101 agaaaaggtg ggagaaagtg aaaatattta gacgaatact aattggctta gtcatcattt
    38161 taggattact cggtggagcc ggttattact atatacagca aaatacgtac gaaccatcgt
    38221 cagaagcgca agtgttggta aaagaaggaa aaaacaaaga agattactta ttttttgaag
    38281 gagagaaaaa gaatcatgga aagtctccat tacttatttt ttatcctggt gctctcgtag
    38341 agccagaaag ttatagtatt tgggcgcaag atttagcaaa aaacggcttt tcagtcgcta
    38401 ttgtcaaaat gccacttgat ctagcagtga tgggcggaaa caaagcggat aaggtcaaag
    38461 aagattttcc aaattccgat tatgtgatcg gtggccattc attaggtggc gtaatggcaa
    38521 gtcgttatgt caataagaat cattcagaca aacatttaaa aggggtattc ttcatggcaa
    38581 gttatcccga caaaaaagga acattgacag aatcagctgt tcctgtttta tcgatcacgg
    38641 cgacaaaaga taaagtattg aatcaggaag cttatcaaga agctaagcag tatttaccgg
    38701 aaagaacaga gtatgtatca atcaatggcg gaaatcatgg aggttttggc agctatggtg
    38761 agcaaaaagg agatggagag gcagaaattt ctaacgcaga ccagcaaact gtgatcgtca
    38821 atacattgga aaactggctg aaaaagttga aataatataa atgggtccta tagggtccgt
    38881 gaaaaaacta gtcaattaac tatagatagc aaatgaatct ttcaaaataa gtcgggattt
    38941 cacggaacaa gagaaacaga tcccggctta ttttcaagtt tagagaactt agtgtacgtt
    39001 tttaaagttt cgtttatatt tatattttat tttctaataa aatcgctcga actggacatt
    39061 tttgatacgc tttttttaca tatgtttctt cttccggcat cacgaactgt tgatgagcct
    39121 ctggttcttg ggcaaataaa acgattcctt cttcatcata atcgaaaata tctggcgctt
    39181 cgatttgaca aagaccgcat gcaatacagc gttcaggtac aagtttacat agtagtgaca
    39241 tttctaacca tcctaactta aggagaataa tatgattgtt tttattttat cgctattttc
    39301 atcaggacac aagctgcgga ttagttctct ttaccaactc cttgtgggaa aacgaacgac
    39361 ttcagtactt atttatggat ttacccacga acttttgttt atccataaca gttttccaga
    39421 attgaagcag gataagtttt atcaaatttt gcaaaaaatt gcgcagcaag gttggatcga
    39481 aataaatgaa aatgaagcaa agctaacgcc tgcaggggct gatatgttgt ccgaacatca
    39541 aatagaacat actggtttgc gttttgaccg ctatggcaga acaggggaaa ctagttggcg
    39601 gctcattaaa tttgctgttc aagtaatttc taatctggca acgggtattc aagactatct
    39661 tccagcagag acaagtcctt tttatacttt ccaactaaaa aaatggctta gtgaaagcca
    39721 attgccacgt gaaattttga ttgatactgc gtatgagagt ttggtgcagc tgttttcgga
    39781 gataccagaa gaagcagcag attttttagc taaccaatta tctggaaatg agcgaacagg
    39841 gctgcttcct tatcagctgg caaaaaacaa tgatgagtca gcggtttatc tgcaacaaag
    39901 cagatgtatc caccttttgt tagcacaaat cgaaaaacgg ccagattcgc tttggcacgc
    39961 tttgatcgat tcattattac agcaaaattt caatcaaagc atgatgatca ccaagcagat
    40021 gtttatgaat ggtcagacaa ttgaccagat catggcaatc cgtcatttaa aaagaggaac
    40081 agtaacagat catcttattg agtgggcctt gttttttgat gattttccat acgagtggat
    40141 tctctctgca gaaacaatcg aacgtttgga accaaataag gattccgtgc gagagtggcg
    40201 ctttagcgaa tggaatgtag atggacaatt agattatgga gaatttcgtt tgtatcaaat
    40261 ctatctattg agaaaggaag cgatacagaa tgttaataaa tgaattaaaa aaatattttg
    40321 gatttgattc atttcgtccc ggccaagaag aaattattca ggcgttgctt gatggagaag
    40381 atactctcgc tattttgcct acaggaacgg gaaaaagcct ttgctatcaa ttgacggggt
    40441 atttaatgga aggtctggtc atcatcgtgt caccgctgct ttctctaatg gaagatcaag
    40501 tgacgcaatt acaaaaacga ggggagaaac gagtagctgc tttcaatagt ttactttcgc
    40561 aatctgaaag aagatacgtc ctccgtcatc tatcgcaata taaatttttg tttttgagtc
    40621 ccgaaatgtt gactaatcct tctcttttag agcatctgaa aaaacaaaat attgcgttat
    40681 atgtagtaga tgaggctcat tgcgtgtctc aatggggagt tgatttccgt ccagaatatc
    40741 aacagttagg aaaaatccga aagcttttag gaaacccggt cacactggca ctaacggcga
    40801 cagcaacaga tttagtagca agagatatac gtcatgtact atttgatcga ccaccaaaag
    40861 agatcagaca atcagtcaat cggcaaaata tctcgctttt tgttcggaaa acgcagcaaa
    40921 aagaacaaga acttgaacac ttcatggaaa gagcacaagg agcggcaatc atttattgtg
    40981 caacaaaaaa agaagtcgaa cgattgtatc atttattcag ggaacgtttt acagttggat
    41041 actatcacgg tggtcttgat gcagctcaaa gaagacaact tcaacagcag tttgtaaaaa
    41101 atcagctgca gtttctcatt gctacgaatg catttggtat ggggatcgat aaatctgata
    41161 tccgttatgt ggtccattat gatttgccag acagtctaga aaactatgta caagaaatcg
    41221 gtcgagcagg cagagatcag gaagaaagtg cagcaattct tttttatcaa agtggggatg
    41281 aacgtatcca ttatttcttt aatcaacttt ctagagaaca gcgacaaagt tttgagcttt
    41341 atttggaata tgccacagag caagctccct ttgatgagct gcaaaaaaaa tggatggagc
    41401 tgatccagca gtctgaaaaa ccagaaaatt gggtggaacg gctcaaacgt caggaaaaag
    41461 aaaaagaatt ccgcctgcag cagatgcttc gctatattaa tgaagaaaac tgccgcagaa
    41521 aattcatctt agcttatttt ggcgaaaaac tctccgaaaa gccgcagaat tgttgtgata
    41581 ttgatggagc aaaagcagaa cttctgccaa agaggaagct tttttctaag actgaacaac
    41641 ttcattggga aacgatttta ctaaatttat ttaaaaaaaa catggaacat tgatttttta
    41701 ggacaagaca ctttttccta tggtataata cacacgatag agtttaggag gtaaaacagt
    41761 gagcagaaaa gatagacatc aatctacaga aaataacgaa acacaagaac catgggaaca
    41821 accaatctac gatacagatg atgaagtttc atcaagaagt tctcagagac aacaaaagaa
    41881 aggaaataca ttatttcttt cgctattttt gattttactg gtcttatgta tcgctattcc
    41941 agcaggtgct tatttctgga tcagaaatgg atcaagcaac aatgcaacag cagcaacttc
    42001 ttctgaaact tcgacatcag tagtagaaag ttctacaagt acaacaaaag aggagtcaac
    42061 tgagacttca acagtagaat ctagtacaat cgcggaagaa tctactgtgt ctcaagatac
    42121 aggtgtaacg tcagaaagca gtattgcagc agagacaaca ccgacttcct caagtgaggc
    42181 aacttatcaa gcaggggatt caagcagcaa tgcagcagaa acaacacctt cttcatcagc
    42241 tgcagccggt tcaacaacga ctgttcaagc aggcgaaggt ccaaaacaag tggctgagcg
    42301 tgcagggatc accactgatc aattgtttga attgaatggc ttagatccaa acaactttat
    42361 gctttaccct ggtcaagaat taagaattaa ataataatag ccgcgctttg cggctttttt
    42421 tctgagaaaa aggagttata tcatgacaca aatcagtatt gcaatcgacg ggcctgcttc
    42481 atctgggaaa agtaccgttg ccaagatatt agcaaaagaa ttgaattata tttatacaga
    42541 taccggtgcc atgtatcgcg cagtaactta tttagctatt acgaacaatg tatctttttc
    42601 agatgaagaa gggctagttt cgctgatcaa aaagtatcct atctcttttt cgcaacaaga
    42661 agatggacag catgtattca tagcatcaaa agatgtgact ttagaaatcc gtcaaccaga
    42721 tgtcacaaaa gctgtttctg aagtctctgc acatggaaaa gtcagagaag aattagttgc
    42781 tgtccaacga aaaatagggg aacgcggtgg cgttgtcatg gacggaagag acatcgggac
    42841 agctgtatta ccgaatgcag aagtgaaaat ctttttagta gcaagcgtaa gtgagcgtgc
    42901 agaaagacgt ttcaaagaga accaagaaaa aggaattccg actgatcttg aaacactgac
    42961 gaaagagata caggaaagag accattatga ttcaacaagg gacgtctctc cgctgaagca
    43021 agcagaggat gctgttcgta tcgatacgac tggcaaatcg attccagaag tcgttgcagc
    43081 aataaaagct gttgttttga aaaaaggtta tcttcttttt taaattaaga aaaaaaatga
    43141 aaaacaattg ttttttcaag gaaaacgaac gaaatcgctt tatttttttt gaataatcaa
    43201 ttaaactaaa aacagtaccg tacttacggc agaatgttgg ttaggaggac aagtgttatg
    43261 acagaatttg atcaaaatca agagaacaac cagtcaatgg aagacgcaat gaaaagcgta
    43321 caagaagtaa aagttggcga tgttgttaag ggcgaagttt tagtcattga agacaaacaa
    43381 gtgattgtcg gaatcgaagg ggctggagtt gaaggagtcg ttccggcaaa agaattatca
    43441 actttgccgg tagaagatat cagcgaagta gtaaaagtcg gtgatgtatt agatttagtc
    43501 gttatcagta cgatcggcaa agataaagaa aacggcagct atctactttc aaaacgccgt
    43561 ttagatgcta aaaaagtttg ggaagatatc gaaagagatt tccaagaggg caaggtcatc
    43621 gaagcacctg ttacaaatgt tgtcaaaggc ggtttagtcg ttgacgtcgg tgtacgtggt
    43681 tttgtccctg catcaatggt ggaagatcat tttgttgcag acttttcaga ctataaaggc
    43741 aaaacattga gctttaaaat catcgaaatc gaaccatcag aaaatcgttt gatcttatcg
    43801 cataaagcgg tagtggaaaa cgaaagagaa gaaaagaaaa aagaaattct ttcaactatc
    43861 catgagggag atgttctcag tggacgtgtt gcacgtctta ctgattttgg cgcatttgtc
    43921 gacctaggcg gaattgacgg tctagtccat gtctcagaga tttcacatag tcatgtagca
    43981 aaaccaagtg atgtattggc tgtcaatgat gatgtaaatg tcaaagtgtt gtctatcaat
    44041 cctgaacaag aacgtgtttc cttgtcaatc aaagacacac agcctggacc ttggtcagaa
    44101 atcgatacaa aagctccagt tggcacagta ttagacggaa cagttaaacg tttgacaagc
    44161 ttcggtgcct ttgttgaagt gttccctgga gtagaagggc tagtccatat ctctcaaatt
    44221 tcacataaac atattgcgac accacatgaa gtgcttcatg aaggtgacga agtgaaagtg
    44281 aaagtattag agatccatcc agaagaacat cgtattgcat tgagtatcaa agctttagaa
    44341 gaaaaaccag cttcagaaga agaaccaaaa aatgttgagt cttatgaatt accagaagaa
    44401 aatactggat ttactatggg agacatttta ggtgatgcgt tgaaagaaga agtagaagaa
    44461 aaagcaaccg atgattctga aaaataattg attttgtatc tgtgactgag atgaaagcag
    44521 ctgcttttgt ctcagttttt cttttgaaaa gcgattagta tttcaataag gaataaaaat
    44581 acttcgttta attgacaatc tatgcttgaa agttggcaat gcattcgtta aactaagtga
    44641 gattggaaac ttgtgaggag gaaataatat ggcaaatcca acaattgcaa tcgttggccg
    44701 cccgaacgtc ggtaagtcta cgatttttaa ccgcatcgct ggtgagcgta tctcgattgt
    44761 cgaagatact cctggagtaa ctcgtgatcg tatttatgca aaaggtgaat ggttaggtcg
    44821 agaattcagc gtgatcgaca caggcggaat tgatcttggc gatgaacctt ttatggatca
    44881 gatcaagcat caagcagaaa tcgcgattga agaagcagat gtaatcattt gtgtagtcag
    44941 cggacgcgaa ggcgtgacag acgcagatga gatggttgcg aaaattttat acagaagtaa
    45001 taaaccagtg attttagcag tcaataaagt agataatcca gaaatgagaa acgatatcta
    45061 tgaattttat tccttagggc taggcgatcc ttatcctgtt tcaggaagcc atggactagg
    45121 gattggtgat attttagatg aagcagtgaa gtatttctct gaagagactg aagaagagga
    45181 cgacgacacg attaagttta gtttgattgg tcgcccaaat gttggtaaat cctctttgat
    45241 caatgcgatt ttaggagaag aacgtgtaat tgtttcggat attgaaggaa cgacccgaga
    45301 cgcaatcgac acccacttcg tttctgaaag tggtcagaaa ttcttaatga tagatacagc
    45361 tggtatgcgt aaacgcggaa aagtatacga aaatactgaa aaatacagcg tgatgcgtgc
    45421 aatgcgtgcc attgaccgtt ctgatatcgt tttgatggta ctgaatgctg aagaagggat
    45481 tcgggaacaa gacaagcgag tagcgggtta cgcccatgaa gctggtcgtg gaattattat
    45541 cgtcgtgaat aaatgggatc ttgtaaagaa agaaaccaat acaatgcgtg atttcgaaca
    45601 agagattcgt gatgaattcc ggtatcttga ttacgctcca atcgtttttg tttctgcagt
    45661 gactaaacaa cggttggaac gtctgcctga aatgatcgaa caagtaagta tgaaccagaa
    45721 tcttcgtatc tcttcggctg tgttgaatga tatcattatg gatgctgtag caatcaatcc
    45781 aacaccaaca gataaaggaa aacgcctgaa gattttttat gctacacaag tcgctgtcaa
    45841 accacctaca tttgtcgttt ttgtcaatga agaggaattg atgcattttt cttatgaacg
    45901 tttcttagaa aatcaaatca gaaaagcatt tacttttgaa gggacgtcca tccgaattat
    45961 ccctcgtagg agaaaatagg cattttacca tatcgacgtt tttttttcaa aagaaaaaga
    46021 cagtttcgtt tgttacgttt ctatgactaa aaaaagcatg tgaaaattgg taaaaacctt
    46081 gctattaagc ggttcatatg atatcgtgat aacagaatat tgaagttaat caatatttct
    46141 ttttaaactt ctggaccact cagaaagttt taactaaatg atatttctat catatctctt
    46201 gtggaggagg tgaatctatc catggcaaac aaagcagaat taatcgaaaa agttgcttca
    46261 gctactgatt taactaaaaa agatgcaact gcagcagtag atgctgtatt ttcaactatt
    46321 caagatgctt tagctaacgg cgaaaaagtt caattaatcg gttttggtaa ctttgaagtt
    46381 cgcgaacgcg cagctcgcaa aggtcgtaac ccacaaactg gtgaagaaat cgaaattcca
    46441 gctagcaaag tacctgcatt caaaccaggt aaagcattaa aagacgctgt taaataagac
    46501 agttgaaaaa gcgcttgtgg ctctagggct acaagtgctt ttctttttat ttcctagcag
    46561 aaaaggaata aataagagac agagattccg aatccgtatg tcatacatag aaaacacatg
    46621 tattttattt aatttttatt tatttgcaag ttatgaggtg agctttgctc ttatcttttc
    46681 tagaatttgc ttactaatgg taaaataaaa ataagaagag gagggatgcg tatggaactt
    46741 gattatcaag tagaagatgc gttgacgaaa gtgttttacg gagaaacgct agtcagtcag
    46801 ctaagagaac tttccattga tcagccaatc ttatttttaa cgaatcagcg ctattatgat
    46861 ttgtttgcag ataagatcaa cccgttattt gcaaatcaag caaacaatga ctggtatatt
    46921 tgtgccaaca ctcaatgcaa tcatttaaca gaattaaaga acctgcttga tttcgtcaaa
    46981 cgctatcccg aaaatcaatc catgtttatc atcggatttg gaaatgaagg gatcatggat
    47041 ctggcaggtt tttttcaaaa acatacaacc cttgattcat cactatggat gatccctgta
    47101 tcgatccgtt ctctaagccg agcattaaca ccgcagcgga cgattttgca gctaccaaat
    47161 cgtgtagtgc tgcaaaccat caatctgcca gaacgcatcg tttatgatca aacaatcagc
    47221 gagcggcagg tggacggaaa gcagattgac tttttgactt ttatctgttg cggaatgatt
    47281 tgtgatcatc gttttttgca aaatctgtat aaaaactatc caaataaaaa gcaattaacg
    47341 actcggccgt ttgccgggat gatcgaagaa atgatctatt tttatcaatt agaggcagaa
    47401 aacctgtcaa attatggtaa actgtttgaa caagcttttt atctgaccga aaatggccat
    47461 ctactttctg ctagtatgaa aaaattttta ggaatgatgt ttcatttcgt gtggaatctc
    47521 caagcatatc cttttgattt ccaactaaaa aacttttttg tgtggctgga cttgcttggc
    47581 tatccaatcc actggctgga acagatttca aagatggagt atttggaaaa agttcttttt
    47641 ttggcagaaa aaagcaaaaa aatacttgtt ttagaaaaaa taggtataat agacggatac
    47701 caacatccaa acgaaaaaga attattgaga acaatgacgg tatttcaaca aatcgtcagt
    47761 gagataagag ggaattgaaa tgaatacaaa tagcgaaaaa atgttgcaag ctttatcaga
    47821 tgaagacttg tcacaagcac aaatcttttt agaaaaagca ttaaaagaag acccagcaga
    47881 tatattagct gaactaggcg aagaattgct ggctattggc tttttgatcg aagcaaaaca
    47941 gatttttgaa caactgatca accaataccc agaaaatgac ggactgaaca tcccgttagc
    48001 agaaattgca atcgaagata atgagatcga cttagctttt gaatacctgg aaaaagtgga
    48061 taagcaaagt gattactatc cgcaaagctt attggcgata gctgatttgt accaagttat
    48121 cggaatccct gaagtgagtg aagcaaaatt gaaagaagca gctgggttgc ttcctgatga
    48181 accattgatc cagtttgcgc tagctgaatt gtatttttca gttgatcgtt tcaaagaggc
    48241 agcagtggtt tatgaaacat tgttggcaag cgggataaat gaaatttcag gaatctcgat
    48301 gcaggaacgt ttaggacaat ccttaagtat gcaaggagaa ttcgaagcag ctatcgtacc
    48361 tttagaagaa gcactgaaag aagaaaggac agatgatcga ctgttccagc tagcttttac
    48421 gaatcttcaa ttgaaagaaa atcagcaggc aatcaactat ctggaagaac ttcgagaagt
    48481 taatccgcat tatcaatcgt tatatctata tctaggacaa gctcttcaag aagaagaaat
    48541 gattgaagaa gcacaaacag tgttagaaga aggaatcaaa gaaaatcctt accaagtcga
    48601 actttatcac ctggcctcgg aaaatgcttt ccgtctgcat gatagacagc gagcagaaga
    48661 acttttactg aaagctttag aagtaggaga caaacaagat gaaacattac ttactttaag
    48721 taatctttac ttggatgatg aaagatatga agatgtgata caagtgatca atcagatgga
    48781 agaaactgct aatccttatg cagaatggaa cctagcccat gcttataatg aattagaaga
    48841 ctttaccgtt gcagctgtac actatgaaca ggcttatcat gaactatcac atgaaccaga
    48901 tttcttgaaa gagtatgcct ttttcttaag agaagaaggg cagctgaaaa gagcgcaaga
    48961 attattgact tattatttaa cacttgaacc aggagatatg gaggcactgt cattattaga
    49021 tgacctaaca gagaggtgag aaaaatgcta attgatgtac gggaaaagaa gaatttttta
    49081 acgtggatgg tcaatcatgt atcttttagc cgaagagaag ttttttggat cttgaattat
    49141 cttgccaatc atgaagcgat tttaagcaat gtccactttg ttgaaggggc gaattttgcg
    49201 gatcgaggac tgcagatcag tgatctatct gttgaagggg aacccatgaa attgtttttg
    49261 cagggaaaag aatttacgga cacagaccag atttttcatg aaatccgttt gaattggaaa
    49321 aagcctttat atgtggaatg tatattcgaa aatgcttggc agacaagaga atatctatct
    49381 atcttagaag acaatccatt ttcttcttgg gattcttcag tcagtgaaga agatactgaa
    49441 ttaattgaac gatattttca agaaaaagaa gaagaggcaa aactaaaaat gctttatgct
    49501 caaatcgatc aagcactaga agatggagat agggatgctt ttttgaaatt atctgatgaa
    49561 gttaaccgaa agatcctttt gcagacacaa aagaaaagta accgggtaag tgaacataac
    49621 aattagttaa aaatagtata gtactaaaaa agacaaaggt cagctggatg tggaatcttt
    49681 cctcatagcc tatctttgtc ttttttctta tagttttttc agtaatcttg tctgtggctt
    49741 caaataagta aagccttgcc cttccacttc atgcacattg atgattgaaa ggaaagcttt
    49801 ttcgtcaatc tcgtggacga tcctcttagc ttcagttaat tcactagagc taacgaccat
    49861 atagatgatt ttttttgaat taccagagaa tgcgccttct ccagaaagga aagtcacacc
    49921 tctttgcaga ccggtcatca gtagtggtgc aatctcttcg cttttgttgg agacgacaag
    49981 tagacctttt gcagaatatc cgccatctaa gatcgaatca atcacacgac tgaaaacata
    50041 cgaaacaatc aacgtataca tcatacgttt caagtcgata taggttaatg aagcaagtaa
    50101 gacgatgata tcaaatgcca atagggaacg ccccatagtg atgccgtaat tttgttctaa
    50161 aatccgagcg ataatatcac tgccgcctgt tgttccaccc atgcggtaga cgatcccgct
    50221 tcctacacct gcaactaaac ccgcaagtag agaaacgatc agtagatcat gctgtaaatt
    50281 gatttctagt ggaacccttt gccagatcca taaaaagaat gacaaagaaa gtgtacctag
    50341 aacagtatag gccaaggaac gtttaccgag gatctttcct cctagtaaaa tcaatgggat
    50401 gttgattaat aaagtcgtat aagctggatc aataccaaat aaagctctta agatcaaggt
    50461 gatccctgag actccccctt ctgctaaatg attagcgatg ttcaagtaaa caagaccaaa
    50521 tgcataaata cttgttccta taatgataaa aagaacttct tttactgttt ttcgtttgac
    50581 catatgaatt ccagctttca attatgtatt ttcacagaat ttccatttag aaattccttc
    50641 ctaaatgtag catggaaatt cgattttctc aattgctctt atctaggatt tgcgttaaag
    50701 ttttggtaag atagttttgg agggaaaaag atggataaaa agcgttcttt atacagtatg
    50761 cagcaggaag tggatgaata tatccagcaa ttcaaggtgg ggtatttctc tccattagct
    50821 cagatggctc gattaactga agaagtaggt gaattggcaa gagaagtcaa ccacacttat
    50881 ggagaaaaaa gcaaaaaggc ttctgaacca gttaactcgg tagcagaaga acttggtgat
    50941 gttttattcg ttacgatgat catggctaat tcattgaata tcgacttaac tgaagttttt
    51001 gaaaaaaaca tggaaaaatt caatcaaaga gaccataatc gctttgagcg taaggacgag
    51061 taaacaagat gaagtggtcc tggccttgaa gactctgaaa cggacttgtc aaacagtcac
    51121 catgcaactg agtctttatc tcaggacaag aaacacgcag gaaaggttgg aaaaatgaaa
    51181 ttaaaacaat tacctctgga ataccaaaag gcaattccag ttctgaaaaa attagagaat
    51241 gcaggatatg aagcttattt tgtaggtgga agtgttcgtg atatcttgct gaaccagcca
    51301 atccatgatg tggatatcgc aacaagcgca tttccagaag aaatcaaaca gctttttcca
    51361 aaaacgatag atattgggat cgaacatgga actgtgttgg tattagaaaa tgatgaacaa
    51421 tatgagatca ccacatttcg gacggaatca acttatcagg actaccgtag accagatcat
    51481 gtcgaattcg ttcgttcgtt ggcagaagat ttgaaaagaa gagattttac gatcaatgcg
    51541 tttgctttga aagaagatgg agaaatcatc gatttgttcg atggtctgca agatctagaa
    51601 gaacagatct tgcgagctgt aggcaatcct catgagcggt tccatgaaga tgccttacga
    51661 atgatgcgcg ggttgcgttt tgtcagtcaa ctagggttca aattagagga agaaaccttc
    51721 tacgccatca aagaaaacca cgagctatta ggtaaaatat ctgtagaacg tataaatgtg
    51781 gaattcatca agatgctctt gggaaatgaa cgggccgcgg gaatccatgc atttattgaa
    51841 acagagtgct acatttgctg tcccggtttg aaagaagaag gcgaagcact tttacgtttt
    51901 gctgaattac catccgtccc attgaaagaa gagagccaag catgggcatt attgatcagc
    51961 caactggatc taaaagaaaa tgagattcgt tcctttttga aatcgtggaa gtgttctaac
    52021 caaatgatta aagacgttca agcacttgtc caaggactac gacaacgaga aactggagct
    52081 ttggaaccat tcgaattgta cttcttaggc aaaaaacatg cactgcaagt agaagaattg
    52141 atgatctatt ttgacgaaac acctcagatg gatgccgtag gagaatccta tgatcgttta
    52201 cctatcaagt cattaagcga attagcagta gatggtaaaa tcttgctaga agaaactgga
    52261 aaaaaacctg gtaagtgggt tagtgaagct ttgcaattag ctgaaaaagc cgtcgtagaa
    52321 caaagaataa aaaatgataa aaaacaggtt ttggattttt tatcgaaaga aaaattgatt
    52381 taaaaaacac tagactgtat ctgctcagct ttgttggcta cttaaagtgg gcagatacgt
    52441 cattagaaga aaaaacataa gatgtgacca cttacataaa tttgtgctaa actgaggtag
    52501 tacaagaata aattacatca taaaggagtc tttttaatga gtaaagaaat gacagcgcta
    52561 aaattttatt ttagaaatgg agaaacctgg acgatcaacc gccgtcatat cggcgatctt
    52621 tggatcaaac aaatcacaac aagttttgga cgaatcaatg gcagtgaatt tgttgaaatc
    52681 catccgtgtg ctggatttaa aatcgaaatc ttccatgaag gggacgcagt tgcgacacat
    52741 gatatcaacc taggcggact ggaaatgggc atgtttaatc gcgcattgaa atacgaagat
    52801 atcgagcgaa tggaaatcct ttaccgaaat ggcacacctg atcttgtgta cttcccatat
    52861 ctagataaag gaacagaagg attagacaat caatatcaat caacaaaaat cagcgaaaaa
    52921 acaggtaact tgtatatcgt gatcaatcca gatcaacgtg tagaagatgt atacggggaa
    52981 tttttcgaat aaatcgattt ttacgcaaaa atgaggctgt gaaagagatg tgtcttatta
    53041 ctactgatag gctgtctctt ccacaccgtt tattcagaat aagggactgt gacaagattt
    53101 ttgtcgcagc cccatttttt tgttgtgata aaagaacaag aaaggcagga atgggaaagt
    53161 aaacatcctt ttgtcagaaa cttttatctt tcttaagttt tacttgatat ctgatactat
    53221 ttggaataga atgaggcgtt gaaaaattta tggaaatttc taactagcaa taaacgatga
    53281 aacaatgaaa tatttttcat ccaaaaagaa ctttgctata atgatggaag tttgtgtata
    53341 ggagatctgg aaaatgagag agagttttga acaacaaaaa aaactgttgc atgatcgcta
    53401 tggcgccctt tcgatggatg accgccgtca aatactttgt aagttgcgta aaagaaatat
    53461 attgatgtat cgtcagttgg aacgtctgaa acatgatctg cttcgtttgg agtcaaaacg
    53521 tgtacagtgt gaattagagg ggaaccaaac acaggttgaa gtagtcgaaa ccaaaatttt
    53581 aaagaaaaaa gaacaatttt taaaaatgct tacacaaaat aaaaaataaa ggggatggac
    53641 gatggaattt gctgagttag ttattgtatt tgcagtaacg atcaccttat cgaacatagc
    53701 tagccgcatt ttgccaatga ttccagctcc actgatccaa attttccttg gagtggtatt
    53761 aggtctgaca gagtggggac aatcaatcga ttttgaacct gaattgttct tagttatgat
    53821 tattgcacca ttgttattta gagaaggaga aaaagcagat atttcttcga ttctcaaaaa
    53881 tttcgggaca attttgttct tggcatttgg aggagtcatt ttgacccttg tcggggtagg
    53941 tgcgacgtta tcattcctgt tgccaagtgt cccacttgct gcctgctttg cttttggagc
    54001 agcgttaggt ccgacagatg cggtagcagt cagttctctt tctggacgag tgaatatccc
    54061 taaaaaggcg atgcatattt tggaaggaga aggtcttttg aatgatgcat ctggtgttac
    54121 tgccttccag tttgctcttg gtgcgctgat caccggatct ttttcagcag taaacgcggg
    54181 gatgagtcta gtagtttcta gtattggggg agctttgatc ggctttttgc ttgtctggtt
    54241 caaacaaaaa ataattcatt tgatcgaaaa agcatcagca caagatgtca cagggtatct
    54301 gctgatcgaa cttttgcttc cttttctagc ctatgtattg gcagaggcat ttgatgtatc
    54361 tggaatcatt gctgcagtgg cagcaggtat tctccaagct tctggtttta ggaaaatctc
    54421 tgtgtttgat gcagaattgt cgagcttgtc ccacagtaca tggacaacta ttgcttttac
    54481 actgaatgca ttagtattta tatttttagg aatcgaattg acgcaagtgt tctcgccagt
    54541 atggggagac ggtttatatc caaacggtct tttattagcg attatcgtat taatctctgt
    54601 catgcttttt gtgatcagat tcatttctat cagtcttttt tatgtattca aagatggcag
    54661 taaaaagttc aaaaaacagt tgaacgagat cttgatctta acatttggcg gtgtaaaggg
    54721 aacagtcagt ttggctacga tttttatttt gccattttca atcaataata tgatgtttta
    54781 ccagcggtct ttgcttttgt ttctgacggc gggtgtcatc ttagtgactt tggtcatagg
    54841 gatcattgtc ttgcctatgc tgacggaaac agaggaagcg aaaagcacgg atttgaatgc
    54901 tttgatgatt ttagaagagg tcgttgaagt tttgcgaaaa gaaataaagg aaattgatcg
    54961 taatacaaaa gaatttttag ccactgaagc agtgatagaa aattatcaag aacggatacg
    55021 tgatctgtac ttggaagatt taaccgatga tgaaaagcaa gaagtacaag aaatacaagc
    55081 actgattttg tcgatcgaac gggatggatt ggatgaaagt tatcgttcgg gcaagctttc
    55141 ttctaatggt tatcgttttt actcccgttt tctttctcga ttcgaacatt ctatcacaag
    55201 ccagattttg tcttttatcg gcttttggtt cattgttgtc cgtaggttaa tgcgaatcgt
    55261 tctccatccg aaaatgtttt ggcaaagaag atatgctgat cgtcagacat ttattagtga
    55321 tgaagatatc caagaaatca gagagacata tcaaaaaaat actcagttga ttattgaaag
    55381 tttagataac ttgactgata tttatgatga aacgatggtt caatttttta tccagcaacg
    55441 caaacttgaa ggaataaaaa tgaactcggg gaatttgatt tcttcgtgga tgatccagca
    55501 agatagtctg tttacgaaaa aaatgctaag aggttattat cttgaaagaa aaatcattga
    55561 tgaatacgaa gttgctgaaa aaatcacgac gttttctgcg aacagctatc gcaggaatat
    55621 caatttgtta gaatcttata cgatgaacaa accatctgat aacttttctt tccgatttgc
    55681 gtttcgagca aagtcaaaaa aaacgagtcc aataaaataa aaaggtaaaa attttcaagt
    55741 tttataaaaa acaaaacata tcagaacagg atcgaatcgg tcctgttctt tttttgagaa
    55801 cacaaaaaga acaaatacca aaaaaatttt cctgaagttc actattttta tgatacaata
    55861 tccaagataa ggagggaaca tgttgaaaga attaaaagta actgacctca aaaaaactta
    55921 tggtgaaaaa gatttgtttg accagatttc ttttttggtc cacgaaaaag accgtatcgg
    55981 gttgattggg acgaacggga cgggtaaaac aagtctctta aatattattg ccggtatcga
    56041 tagcagcgac ggtgatcggc aaacagtgtt ttatccaact gattaccgta ttggttatct
    56101 ttctcaagca gctgaatttt ccgatgaatt aactgttctc caagcagtct ttcaaggaaa
    56161 cagccccttg atccaaactg ttcgagcata tgaggaagca ttgatcgaac taggggaaaa
    56221 cggagaagac cctgatgtgc aaaaacgtta tgctaaagca gaagaacaaa tgaataaaga
    56281 agatgcttgg actacagata ccaacgcaaa gattattttg caaaaattag gaatcagtga
    56341 attagataaa aaaatcagta ctttatcagg tggtcagaaa aaacgagtta gtctggcgca
    56401 agtgttgatt gatgaacccg atttactatt actagacgaa ccaacaaacc atttagatta
    56461 tgaagcgatc gagtggttgg aaaactattt gaaacaatac cgtggcgcac tattgatggt
    56521 tacccacgat cgttattttc tagatagagt agcaaaccga atcttcgaac tttcatttgg
    56581 aaaattatac gagtataaag gaaattatga agcttacgtc ttagaaaaag cagaacgtga
    56641 tcgtgtagaa gtagaacaag aagaaaagcg caagcggctt tataaacagg aattagcttg
    56701 gatgagggct ggcgtacagg cacgtggcac caagcagcaa gcacggatca accgtttcga
    56761 agatctaaag gagaacttgt accaagtaaa ccaagaagat gatttagagc tgaacctagc
    56821 gacacaacgt ttagggaaaa aagtactgga aatcaaagat ggcagttacc gtataaatga
    56881 ccaaacactg ctagaacatt tagatttatt gattcaatct cgggaaagac taggtattac
    56941 tggtaaaaat ggtgcgggaa aatcgacatt gcttaatatt ctagctggca gaattcccct
    57001 ggatagcggt acaatgtcta tcggtgaaac ggttcatctt gcgtattata cacaagaaaa
    57061 tgaagaaatg gcaccagata aacggatgat tgcttactta caagaagctg ctgaagaagc
    57121 aaaaacagca gatggcagtc aaatcggagt agcagaatta cttgaacgat ttttatttcc
    57181 aagatttatg catgggacac tgataggcaa actttcaggc ggagaaaaac gccggttatt
    57241 tttgttgaaa ctgctgattc agcaacctaa tgttttatta ttggatgaac cgaccaatga
    57301 tttggacata gcaacattga cgattttgga agattatttt agaagttttc caggtgctgt
    57361 gatcactgtt tcccatgacc gttatttctt ggacaaggta gcagataagt tattagtatt
    57421 ccaaggaaac ggaaaacaag aactttatta cggaaacatg tccagttatt tactgaagca
    57481 aaaagagacc cagcagcctg ctgaaaaagc gaaaccaaaa atacagagca aggaacctgc
    57541 tgaaaagaag aaattatctt acatggaaca aaaagaatgg gaaacgattg aagatgagat
    57601 tgcagaactt gaagaaaaaa tctcattgct ccaagaagag atgaatcatc aaggagataa
    57661 tttcactcga ttacaagaat tgcaaaacga cgtatctgaa acagaagcac aacttgaaga
    57721 aaaaatggca cggtgggaat acttgagtga atgggtagaa gattaggagg aaaaaggatg
    57781 gaacaagcgt atcttgactt aggaagaaaa ttgttggaag aagggcatcg taaagaagac
    57841 agaacaggca caggcacatt aagcttattc ggataccaaa tgcggtttga tctgcaaaaa
    57901 gggtttcctt tgttaacaac gaaacgagta ccttttggat tgatcaaaag cgaattgctt
    57961 tggtttttaa aaggagatac gaatatccgc tatctattac aaaataaaaa ccatatttgg
    58021 gatgaatggg catttgaacg ctacatcaaa agtccagact ataatggacc agatatgact
    58081 gattttggcc atagatgttt gacagatcct gaattcaatg aagaatataa aaagcagagc
    58141 aagctttttt gcgagaagat cctaaccgat gattcctttg ctgaaacata cggcgatctt
    58201 ggacacatct acggctatca atggcgtcac tgggaaacaa aagacggtgg ctttatcgat
    58261 caaatcaaac aagtgatcga agcaatcaaa aagactcctg attctagaag attgatcgtt
    58321 tctgcatgga atccggaaga tgttcctagc atggctttgc ctccttgtca tacgatgttt
    58381 caattttatg ttcaagaggg acggctaagc tgccaattgt atcaacgcag tggggatgtg
    58441 tttctaggcg ttccgttcaa cattgcaagt tatgcgttgc tgactcacct aattgcacat
    58501 gaaacaggtt tagcggttgg cgaatttgtt cacacattag gagatgctca tctttatatc
    58561 aatcatatcg aacaaatgaa agaacaattg agccgagaaa tacgcgcatt tccgacttta
    58621 gttttgaatc aggaaaagca gtcagtattt gattttgaga tggaagatat ccaattagaa
    58681 ggatatgacc cacatccggc gatcaaagct cctatcgctg tttagaatca gaaaggatga
    58741 gaaaatgtta gctgcaattt gggcacagga tgaaaacgga ttgattggaa aagaagaccg
    58801 tcttccttgg cgtttgccaa atgatctgaa attcttcaaa caaatgacag aagcaaatac
    58861 actagtgatg ggacgaaaaa cattcgaagg gatgggtagc cgtccgttgc cgaatcgtca
    58921 gacgattgtt ttgacgagag attcttccta taaagcagaa ggcgtgcatg tcatgcatag
    58981 tgtggaagac gtgcttgctc tggaaaaaga aacagacggt atctttttca tcgctggagg
    59041 gtcagctgtt tatcaagaat ttctgccatt ttgtacgata ttataccgta cagtcatcca
    59101 tcatgtattc gatggagatg cttattttcc acctgttgac tggtctgatt ggtcactgat
    59161 caatactagt caaggagaaa tggatgaaaa aaatcgttat ccgcatcaat ttgaaacgta
    59221 tcagcgtaat gagaatcctg taaaaagcgg gacaggcgaa ttgacgatat aaaaaagcta
    59281 tggatagact agctgttatt tagagctgtc ccatccatag ctttattttt tttattaagt
    59341 aaagaataaa acagagaagt acataaaacc tgcacctaac ataacaaaca aatgccaaac
    59401 aacatgcata tagcggatag aattcaagct ataaaagaat gcgcctatcg tgtaagaaag
    59461 cccgcctgct accatcaaaa taacaccagt cattccaaga gagtgataaa gaggaacagc
    59521 agccacgaca cagagccacc ccataaaaat atagatgatc gtagatactt ttgatactct
    59581 ctcccgttta tgcaacgtca acgctttata tataatgcca agaatcgcaa acaaccagat
    59641 taatacaaat aaagtccaac ctagccatcc tcggatactc agcaaacaaa aaggagtata
    59701 acttcctgca atcaaaagaa aaatcgaact atgatcgaat acttggaaca ccttttttgc
    59761 ttttgtaaaa atcaatgaat gaaacaaagt agacgttaaa aataaaagaa tcatcatcga
    59821 tccatagata gcataagaga caatatggag ggaggaatgc aagtgggcac cttttactaa
    59881 aagaaccact agtcccgcga tactcaatcc agtcccaatt ccatgggtga ctgcattaaa
    59941 cacttcattt aatatttcat acgttcgatt aaatttcgtt ttttccatga aaacggctcc
    60001 tttactaggt gtaattaatg aaaaatcatt atttgtctgt tatactaaaa atagaaattg
    60061 tcttacatgt agtttaacat gaataaggca tcttgttaaa gaaagagaga gagaaaataa
    60121 tgacaaatgt aaaaatcgta acagattctt catgtacgat ggaaaaaaat gtacgcgatg
    60181 ctttaggcat tcgtgtcatg ccgctatcca tcatgatcga cggaacagta tatcccgatg
    60241 atgaccaact agacggcgaa agatttatgg aaatgatggc gaaagcaaaa aatctgccca
    60301 aaacaagcca accaccaatt ggagaattca tctcgatgta tgatgagcta ggagaagacg
    60361 gcagtgaagt gatttcgatc catatgacaa aaggcttgag tggaacagta gaagcagcaa
    60421 gacaagccag taatctaaca aaaacgaaag taacggttat cgacagtgat accactgacc
    60481 aaggattatc ttttcaggta attcgagcag ctgaattagc aaaagaaggt aaaacagtag
    60541 agaaaatcct tgaagaagtc aaaaaagtac aagacaatac aaaattatac atcggaatat
    60601 ccacactaga taacttggta aaaggtggaa gaatcagccg aacaactggt ttgctgtcta
    60661 gtgttttgaa catacgcgtg gtgatgaatt tcgcccattc tgaattaatc cctgtaacta
    60721 aggggcgagg aaagaaaacg ttcgataaat ggtttaataa tttgaaaaat gaactgaaag
    60781 aattgccaaa tgtgcgacaa atcggtattt cacatgcgga tgcaaaagaa ttggcgcaag
    60841 aattcaaaga gggtttgcaa gaattattcc cggacatgca tatccctgta ctgcatacga
    60901 atccggttat cgctacgcat actggtaaag acgcgtttgc aatcatgtat tatgtagatt
    60961 agtcgaaaag aggataagcc ctcagcctga aaggcttgca gcttattctc tttttcttta
    61021 taaaattagg aaaataattg ttttcttttt tcataaagac tacctttttg ataaaatttt
    61081 tgataacatt agaaccaatc gaatacaaga ggtgaaaaaa tgaaattaac gaaacgacat
    61141 ttgatcctcg ttttgttccc attattaatt gcgatcatca tctatgcagg tttagctgct
    61201 tctataccga aagctggccc actaatgaaa ccagtaacta ctgtgagaga agatccaaat
    61261 aaaaaagaaa aacttcacta catagctatc ggagattcgc tgactgaagg agtaggagat
    61321 caaaccaagc aaggaggatt cgttcccctt gtcgcaaatg atctaaaaga ccgttataat
    61381 ttgaccgcgg ttgaaacaga aaactatggt gtaaatggtg aacgaagtga ccagatctta
    61441 aagcgagtaa aaacaaaaga agaaattcga aacaatatca aaacagctga ttttatcaca
    61501 ctgactgttg gcggtaatga tttgatgaaa gttatccaaa ataatttatt cgggttaacg
    61561 gtcaattcct ttaaaaagcc actgaaaaac tatcaggaaa atgtcacaga actattggaa
    61621 gaatttcgat ccctcaataa aaccgcacct atttacattt taggcatcta caatccattt
    61681 tatttgaact ttcctgagat cactgacatg cagacaattg ttaacaattg gaacaaagga
    61741 acagaaacgg tgacaaaaaa aatgccgaac tgctacttta tcccaatcaa tgatttgctt
    61801 tatcaaggat tgaatcagga agtaggtgtc acagaagaag gaacagatca aacaactagc
    61861 acgacagaaa atctaaatgg catcaaaaat aatgtgctct atgaagaaga tcatttccat
    61921 ccaaataatt taggttatca attgatggca aatgcagtga gagatcagct gatcgagaca
    61981 caaaaccaat ggttagagaa ggaggatgat caataaacat gaaaacaaga tctgacgtat
    62041 cttcaaacaa aacaaagaaa acaaattact ggaaattcgc cttccttatc cttcttgggg
    62101 tagtacttgg ttcgaccgta tttttaggca gccgtatttt tgctaatcgg gaaccagaac
    62161 tcccagaaat accagctctt acggaaagac aaggagatcc tgtcttaacg atcaattcaa
    62221 ataaagaaaa agtcaatcaa atcatcagct ttttccttag tgagtatcaa aaagatagtg
    62281 atattgaata taaattttat ttggaaaatg aagcactact caatggtact ttcgaagtac
    62341 taggttttcc tatcgatttc tatttatatt ttgatccgta tgtgatggaa aacggaaatg
    62401 tccaactaaa agctagaagt ctttctatcg gaacattgag tttgccaatc aaagatgtga
    62461 tgaatatgat caagagaaac tataaattac cagattggat cgaagtcaat acagatgatc
    62521 tgactatcat gcttaggttg gatcaattcc gaatgcaaaa cgggatgtat attaaagcag
    62581 ataaaatcga cttagtaaat gatgacattc ggtttagtct atatttgcca aaagatacct
    62641 ctgatgcttc aaacaactca aataccaaca aaactagcac cagcagtact gaaagcaaga
    62701 caaaagaaaa taaaaaatca tcggatgagt agtattagag ggggaatcat aatggaacga
    62761 gcaatatttg caggaggctg cttctggtgt atgatccagc ctttcgacac gcttcctggt
    62821 atccatacag tgatgtcagg atatactgga gggcatgtac cagatccgac ttatgaacaa
    62881 gtaaaaagcc aaacgactgg acatacagaa gcagtggaga ttatctatga tccaacattg
    62941 atttcatatg aacaattagt agaactttac tggcagcaaa ctgatccaac tgacgctttc
    63001 ggacaattcg aggatcgcgg tgacaattat cgcccggtga tcttttattt gacagaagaa
    63061 cagcggatca ttgctgaaaa aagtaagaaa cagttagctg aaagtggacg tttcgaacag
    63121 ccaatcgtta caaaaatcga accagcccaa ccattttatc tagcagaaga agaccatcaa
    63181 gagtattata aaaagcatcc tgaaaatttt aaaagaaacc atgcacgccg ggctgccttt
    63241 atcgaagaaa attggaggaa cgaagggtga atcgaagctt ttttcattat ttaatgacac
    63301 tgcgaggcgg tggcacacat gatgctttat ctgttttcgc tacagaagcc ggtaaagaca
    63361 tccaatttcc taagcacagc tctagctacg aagaaatttc taattatctg gaactgaatg
    63421 tcgactattt gccgtccatg gatatctttg acgaagcatg ggaaaaatat ttagaaaata
    63481 atcatgacat ataagagata gttttgtact ttatgatcaa aaaagttttt tgtccaatag
    63541 cctaattgaa agaattattt ttatttcagc tatctagtcg catttttttg atatttttct
    63601 tataataaag aaagaatgaa aaaaagaggg atggattgat agagattgac aaaaaaacaa
    63661 cgctatatta atcgcttctg gcttgtattc aaatacttac ttgtttctgt attccttgcg
    63721 ttcatgttgc ggggattttt attcatcccg gtaccagtgg aaggcaattc aatggaaaac
    63781 gtgttgaagc aaggtgatat ggttgtgatg gaaaaatttt cggagatcag acgatttgat
    63841 attgttgttt ttcaattagc agatggcacg atttatatca aacgagtaat cggtcttcca
    63901 ggagaaaatg tcagctacca aaatgatcaa ttgaagatca atgggaaagt agttaaggaa
    63961 ccttatctaa ctaaaaacct gaaatcagat catgcaaatg catcctatac gacggacttt
    64021 acactacaag agctaacggg acaatccaag cttccggaag ataattattt tgtacttgga
    64081 gataatcgaa gagtttcaaa agatagtcgt tcgtttggca cgatcaataa aactgatatt
    64141 ctaggtaaag caagatttgt ctattatcca cttgatgaaa taaaatggat ccagtgagaa
    64201 acaataaagc gtgcagtctg ttgagactgc acgctttatt gtttaatcca aaatcagttt
    64261 actaccttag tacgtaaaat caaacagctc gtttaaacac ttttctagcc tctaaagttt
    64321 tgataaatga ataaacaacc attatttgaa tagggatttg gatcatattc ctgatgaatc
    64381 tacctagaaa aattactttg aaatctgcac caaacaaaac agacatcagc caagaattga
    64441 taaaaaggct gactgggatc ataatcaata attgggcagc gatgacggaa ggcaggttta
    64501 tctttttatg atataaaaat aagccattaa taaatccacc aaggaaagcc gtgataagaa
    64561 aagcagcttg aaacgttcct gaaccaaata tcgtgaaatt taaagtgttc gacaacattt
    64621 caagcaaacc agcccagatc ggtccatata gataacccat aagcgccata cttaaaaatc
    64681 cgaatgtgat ttgaaaataa gcaggaccaa tcgctatctt gtataagatg acatcaaggg
    64741 ctaataaaag agcagaggtc gacaacaatc ttgcattcat ttcgggcaat ctaaaaacgt
    64801 cttttgcaat caaagtgttc aaaatattct ctcctttgtc agttaataat aaaaggatac
    64861 cataaaatca ccaatgtgtc atgacacatt ggtgatttac atacactggc atctaacaat
    64921 taaaaaaagg gtgagaagac tgcttttata aactgtaaaa aagagattgt acgaataaaa
    64981 aaaatagagg cggagaatac ttatcgtatc atccgccttt gtttttttgt tataaaaaac
    65041 gtcagattag ttatctgctt acatcttcat ccatacgggt tctaatgtat ttccgtctag
    65101 atcttggact tctaatgaaa acatttcttc ttcaggcatt cccatatcta cttcgtggaa
    65161 agtaccacca ttttcttttg caatttgagc aaattttttt acttcatccg cattttcaac
    65221 actaaacgaa atcaaagcac cacttgtttt tttattatca gctatttctt tatcttttat
    65281 aaatttttta taaaaatcat gagagagaag cattatccaa aaagtgtcat cccataccat
    65341 tgaaccagct tcttcagtag aaaattcttc atttttttta aaacccagct tttcataaaa
    65401 agcaacggaa cgattgacat cagcaacagg aaaattaaca aaaaccatac tattcatttg
    65461 tttcgtctcc tttctgtttt gttcgtctat catctcttat ttaggaaacg ctgtcaaaaa
    65521 tttagattca tatatagaaa ccaaaataaa aatttgcatc atcaaaaaag tgaagatgtg
    65581 atagaatgag gactggttca ctttcactta actaatagat ggaatttctt tataataaaa
    65641 aagtgaattt atattgaaaa agaaaaaaac aagtaaagcc ggggaatatt ccggtatgaa
    65701 aggaatggag agaacaatga ccatccaatg gtttccggga catatggcaa aagcccgacg
    65761 agaagtttct gaaaaaatga aatatgtgga catcgtattt gaattggtag acgcacgatt
    65821 acctttatct tctcgtaatc caatgctaga tcagatcatt cagcaaaaac ctcgccttgt
    65881 tttattgaat aaagcggatc tggcagatcc acaacagaca aagctgtggc aagaatattt
    65941 tcgtaaacaa ggccatcttg cattagctat cacggcacaa gaaagtaaag gaattaaagg
    66001 tctagtacct gcggctaaag aagcattgaa agaaaaacgg gcaagagatg ctgctcgggg
    66061 gatcaagcct cgcgctattc gggcaatggt cttaggtatt ccaaatgttg ggaaatctac
    66121 gttgatgaat cgactagtag gcaagaaaat tgcacagaca ggaaataagc ctggcgtaac
    66181 aaaaggtcag cagtggcttc gtttaggtaa tgagttggaa ttgttagaca caccagggat
    66241 tttatggccc aaatttgaag atgaagaaat cggcaaaaaa ctagcgctga caggcgcgat
    66301 caaagaccag cttgtccatt tagatgattt agcgatttac ggattagact tttttgcccg
    66361 atattattca ggaagaatca gcgatcgtta tggtttatca atcgagcaag aagaaaaatt
    66421 ggcaccggaa ttactgatgg atattacagc aaaacgtggg tataaagaag actatgatcg
    66481 tggcagcgaa ttagtcgtgc atgatattcg tcaaggaaaa cttggacgat acacactgga
    66541 tcgttgcgaa gaaatggagc aaaaaagtga tgactgaatc catccaaagt atcaaagaac
    66601 gattgaaaag catacagaga atagatgatc ctacactaga atcattaaga aatgatccac
    66661 gcaaaggcgt gcagcaagca ctggcttctt ttgaacgacg attgaaaaag gaagcattaa
    66721 ttaaggaaaa atatgaagaa atgttttgct ttgaaaagca agggtacgca caaggtcacc
    66781 aatggattgc cggcattgat gaagtaggaa gaggaccatt agctggacct gtcgtggcag
    66841 ctgcagttat cttgccgaaa aatgagatca tattagggct gaatgactcc aaacaattat
    66901 cagagaaaaa gcgagaactt ctttatcaac aagttcaaga aaaagccctg gcaatcggga
    66961 tcgggatcgt tgatcagcag acaatcgatg atgtgaatat ttatgaagca agtaaattag
    67021 cgatgatcca agcagtagaa aaattgtcca ttgtaccaag ctatttgttg atcgatgcga
    67081 tgaccttgga tttgcctatt cagcaggaaa aaataatcaa aggggatgca cgatctgtgt
    67141 cgattgctgc tgcaagtatc attgctaaag tatatcgaga ccaattgatg aaagaatatg
    67201 acgtgcttta tcctggatat ggtttctcgc ataatgcagg ttatggaaca aaggaacact
    67261 tagaaggact tgaacgacaa ggaattacgc caatccatcg taaaagtttt gcacctgtaa
    67321 aaaaatattt acaataaaaa acagccgctt ttacgtatct tattagaaag agagacgaaa
    67381 ggcggtttta tgatgtatca aatagaagaa aatttattga aacttgctta tacaaaagga
    67441 atcagtagtc aaggaaaatg ggcacttgct aactggatga tgcagtatcc ttataaagaa
    67501 atcggatttg aagacatcat taagataggt aaaataacca aacaccggga gctgtttcgc
    67561 aaaagttggc aggaaattca cgctaactgg ggcaaaatcc aatcgaaaca gtcgtttatc
    67621 acttgctttg atcccaaata tccccctcag ctgcttcact tgacctatcc gccaattgta
    67681 ctgttttata atggtgatct atctttactt tcttgtaaca tgttaagcgt tgttggtgga
    67741 agacacactt ctgctttagc caaaaaaaca gtttattatt tactaaaacc agtgatagaa
    67801 gctggatatg tgatcgtcag tggctgcgcg aaagggatag atacatacgg tcaccaagca
    67861 gcaatctctc atggtggacg gacaatcgca gtcattggaa cagggataga gcaagtctat
    67921 cctgctgaaa atcgtgactt gcaagaggtg attgcaaatg atcatcttct tttgagtgaa
    67981 tatcctcctc aatccggccc gaaaaagcat tgttttccaa tgagaaatcg gattatcgca
    68041 gctttgtcta aaggaacatg tgtctttgaa gccaagaaaa aaagcggctc tctgataaca
    68101 gcacagcaag cattggatct tggaagaaca gttttttctg ttccaggaaa tattttaaca
    68161 gaacattcta ccggatgtca tcatttgatc caagacggtg cagtttgcac ggtttcgggt
    68221 gaagatatct tggcagaatt taaagaataa caccatactt ttcatttgct tttcttgaca
    68281 aacgttttct gaatggatat gattagctac gatttgtatg aatcctcttt taatagtagg
    68341 aacacgacaa tggaaaggag tccattcagt aatggcgtat aaatatttag tgattgtaga
    68401 atcacctgca aaagccaaga caattgagaa atatcttgga cgaaactata aagtaatggc
    68461 cagtgttggc catataagag atttaccgaa aagtaaaatg ggtatcgatt ttgaaaacaa
    68521 ttatgaaccc cattatattt ctatacgcgg aaaaggcgat gtcatcaaaa gcctgaaagc
    68581 cgcagcaaaa aaagctcaaa aagtttactt ggcaagtgac ccggatagag aaggagaagc
    68641 gattgcttgg catttagcgt acctacttgg gttggatctg aaagaaaaaa atcgggtggt
    68701 cttcaatgaa atcacaaaag acgcagtcaa agcagctttt aaggaaccaa gaacgatcga
    68761 tgtagattta gtagatgcac agcaagctcg tcgtacctta gacagaatcg ttggttattc
    68821 gatcagtcct attctctggc gtaaggtcaa gaaagggtta agtgcaggac gtgtccaatc
    68881 tgtcgcttta aaaattatta ttgaccgtga aaaagagatc cgagaatttg ttccagaaga
    68941 atattggagc atcgacggta attttaaaaa agctcgcaag aaattcaaag caaatttctg
    69001 gggaatcgac ggtaagaaaa agaaattacc agatgcacaa agtgtaaaag aagtcactgc
    69061 tagaattgat ggaacagact tcgaagttaa aaaagtagaa aagaaagagc gcaaaagaaa
    69121 ccctgcgaat cctttcacta caagtagcct gcaacaagaa gctgcccgta agttaaactt
    69181 tcgaacaaga aaaaccatga tggtagcaca gcaattatat gaaggaatca ctttgggtaa
    69241 acaaggaaca gtcggcttga ttacatacat gcgtactgat tctacccgga ttgccgatac
    69301 agcaaaagca gaggccgcag catttattga agatacgtat ggaaaagaat tttctagaca
    69361 tgatcaccga aagatcaaaa atgctcaagg cgcacaagat gcccatgaag ctgtgcgtcc
    69421 aacgagtgtg cttcgtacac cagatgaact caaacaatat ttagataaag atcaattgaa
    69481 actttataca ttgatctggt cacgttttgt ggctagtcag atgacaccag ctattttaga
    69541 tacaatgaaa gtgacattag aacaaaatgg tgtcatcttc attgcgaacg gttcaaaaat
    69601 caaattcaaa ggattcatgc aagtgtatgt ggaaggtaga gatgacggca aagaagagaa
    69661 agaaaatatt cttccagaac ttgaagaagg cgatgtagtc caatctgttg atattgaacc
    69721 aaaacaacat tttactcaac caccagcacg ttttagtgag gctacgttga ttcgtgcact
    69781 agaagaaaac ggggtagggc gaccttccac ttatgcacca acacttgata cgatccaaag
    69841 acgctactat gtaaaattga cacaaaaaag atttgagccg actgaactag gagaaattgt
    69901 caattcatta atctgtgaat tcttccctca aatcgtcgat atccatttca cagcagaaat
    69961 ggaaggagat ttggataaaa ttgaagaagg cacggaagcg tgggtcaaag tggttgatcg
    70021 tttctataaa ccgtttgaaa aagaactgac gaatgcggaa gaaaaaatcg aaaaaattca
    70081 aatcaaagat gaaccagcag gattcgattg tgatgtctgt ggacatccga tggtgataaa
    70141 attagggaaa tatgggaagt tttatgcttg cagtaatttt cctgattgtc ggaatacgaa
    70201 accaatcgta aaagaaatcg gtgtgacttg cccagtatgt catgaaggac aagtcattga
    70261 acgaaaatct aagaaaaaca gactattcta tggttgttcc cgtttcccag aatgtgaatt
    70321 cacttcttgg gataaaccag tgggcagaaa ttgtccaaaa tgtgacggat atctggtcga
    70381 gaagaaagtc aaaggcggta aacaagtagt ctgtgtaaat ggagattacg aagaagacat
    70441 ccaaaaataa tagaaaagag gttctttcat tgaaaatggt aaatgttgtt ggagcagggc
    70501 ttgccggaag tgaagctgct tggcagatcg cacaagcagg agtccctgtc cgtttatatg
    70561 aaatgcgtcc agtcaaatcg actgaggcgc atcacacaag taattttgct gaactagtat
    70621 gttccaattc attaagagga aacagtttgg ctaatgcagt agggttattg aaagaagaaa
    70681 tgcgccgatt gaattcggta gtaatcactt cggcagatga aacagcagtg ccagctggtg
    70741 gtgccttagc tgttgaccgc gattcttttt ctgaaacaat tacccgaaaa gtcaaagagc
    70801 atccattagt aacagtgata aacgaagaaa ttactgaaat accggaagga atcaccgtag
    70861 tagcaacagg acctttgact tctcatccat tagcagaaag catcaaagca ttcaatggtt
    70921 cagatggttt ttatttctat gatgcagctg caccaatcgt agacaaagct acgatcgata
    70981 tggataaagt atatttgaag tcccgctatg acaaaggaga agcagcttac ttgaactgtc
    71041 cgatgacaga agaggaattc aaagctttcc atgaggcact aatcaatgcc gaagtggcag
    71101 aacttaaatc ttttgagaaa gaaaaatatt ttgaaggatg tatgccaatt gaagtaatgg
    71161 cacagcgagg attcaaaacg atgcttttcg gacctatgaa accagttgga ttggaagatc
    71221 ctaaaactgg caaacgtcct tatgctgtta tccagctgcg tcaagacaat gctgcagctt
    71281 ctttatacaa catcgtgggc tttcagactc acttgaaatg gggcgagcaa aaacgtgttt
    71341 tccggatgat tcctgggtta gaaaatgctg aattcgttcg ctatggtgtg atgcaccgaa
    71401 acagctttat gaattcgcca gaattgctga aaccgactta ccaatcaacg aaacgcgaag
    71461 atctgttctt tgcaggtcag atgactggtg tagaaggcta tgttgaaagt gcagcgagcg
    71521 gactacttgc tggaatcaac gccgcacgct tagcaaaaga aatggaacca gtcgagtttc
    71581 cacaagaaac agctattgga agtatggctt actacattac tcatgcggaa ggaaaacatt
    71641 tccaaccgat gaatgccaac tatggtctgt ttcctgaact gccagagcgc attcgagaca
    71701 aaaaagcaag atatgaagca attgcacaac gagctttaaa agctaatgag gcaataaaaa
    71761 cagaattaga aaaaactgca atcccaaatg agtaaggaaa ctccatgaag agggtgagac
    71821 ggatagtctc gctctttttc ttgcgttttt cttaagaatt tctcaaacaa aatgtgagga
    71881 attgcgccaa ttgtgaacaa acttagaaat attggttaaa ttctgacaat ttatttgtct
    71941 ttttataaac gctgtgctaa attattctct gggacaaaag gaaggtgaga aaatgacaga
    72001 agccacgtgg gaacaaaaat ttttgcgtta tttgattgtc gaaagaggat attcagaaaa
    72061 aacgaaagaa gcatatgaag aagacattct ccatttcaaa cattttttgg aggaatctgg
    72121 agacgctgat ctgctgaagg cggaatattt tacggttcgt gtctatttga gtgcattagc
    72181 tgatcatcgt cctagctata gcagaaattc tatcagtcgc aagatagcca gcctacgttc
    72241 gttttatcag tatcttctca aagaagaagt gataaaagaa aatccatttt cttacgttca
    72301 tctcaaaaag aaaaatttga gactgccacg ttttttttat gaaaatgaaa tgcaagcact
    72361 ttttgacagc gtagcaggag atagtccttt ggatttgagg aaccgcgctt tactggaagt
    72421 cctttacggg tcaggcatcc gtttaagtga gtgtagcagt ttgtcggtat cagacattga
    72481 ttttgattca gaggtcatgt tgatccatgg taaagggaac aaagagagat atgctcctct
    72541 aggttctttt gctcaagatg ctctgcaaga atatttcagg gaaggccgcc aggtattgat
    72601 gacaaaatat cacgaagagc atgatatcgt ttttgtgaat catcatggaa aaccaatcac
    72661 gcctactgga attgaatatg tcttgaatca aatgatcaaa aaaagcagtt tggatagtga
    72721 tatccatcct cacatgctgc gccatacatt tgctactcat ttattaaata atggggcaga
    72781 tttgcggaca gtccaagaat tgctaggaca tgccaattta tcgactaccc agatctatgc
    72841 ccatgtaaca aaagaaagtt tacaaaaaaa ctatcgttct ttccatccaa gagcgtaaaa
    72901 ataaggaaaa gaacaaattt aggaggaaac aaacatggtc gaatcaccat ttcattcaac
    72961 cactatctgt gccgtcgaaa aagacgggaa atttgctatg gctggagatg gccaagtaac
    73021 aatgggtgaa tctgttgtca tgaaaggcac tgcaaagaaa gtacgacgta tctacaatgg
    73081 agaagttgtt gtaggtttcg ccggaagtgt tgcagatgcg tttactttag aagaaaaatt
    73141 tgaaggcaaa ctaaacgaat ataacgggaa tctacaacgc gcagctgtcg aattagctca
    73201 agaatggcgg acacagcaat ctatgcaaaa acttgaagcg atgctgatcg taatgaatga
    73261 taaagaaatg cttcttgttt caggtacagg agaagttatc acaccagatg atggtatctt
    73321 agctatcgga tcaggaggga attttgcgtt ggctgcagcc cgagcaatga aaaactatgc
    73381 tcaaaacgac atgagtgcga aagaaattgc agaaaatgcg ttaaatattg ccgcagatat
    73441 ctgtatcttt actaatcata atattatcgt agaagaactg taagaggtga gtagatatgt
    73501 atgaattaaa acaaacacca agacaaatcg taagtgaatt agatgaatat atcgtaggac
    73561 agcacgcagc aaaaaaatct gttgccgttg ctttgcgtaa ccgctatcgc cgtttgcagt
    73621 tagatgaaaa aatgcaacaa gatattacac caaaaaatat gctgatgatc ggacctacag
    73681 gggtcggtaa aacagaaatc gcacgacgtt tggcaaaaat cgtcaatgca ccatttgtca
    73741 aagtcgaagc aacaaaattt actgaagtcg gttatgtcgg aagagatgta gaatcaatgg
    73801 ttcgggattt agtagaaaat gcgatccaaa tcgtagaaaa agagcagtac agccgtgtat
    73861 acacaagtgc taaaaaaaat gcagaaaaac gtttggtaaa aatcttggta cctggtatca
    73921 aaaaagaaca gaaacaatcc actaatcaat ttgaacaaat gatgaatctt tttaatgcta
    73981 gtcagcaaaa taccgctgaa ccgcaagaag aagtgacaga agatattcga atcaatcgcc
    74041 gtacagtctt ggaacaacta caaaagggtt tactggatag tcgtgaagta accattgaag
    74101 ttgaagacca aaagaaacct gcaccaatga tgaacaatgg attggagcaa atggggatcg
    74161 acctaaatga aactctaggt gctttgacac cgaagaaaaa agtggaacgg acggtaacag
    74221 tcaaagaagc tttagagtta ttgatcaagg aagaatcagc aaaattagtc aaggatgctg
    74281 atatccatag tgaggcaatc aaattagcgg aagaaagtgg aattattttt atcgatgaaa
    74341 tcgataagat cacttctaaa tcacagcaat ctggagaagt atccagagaa ggcgtccaaa
    74401 gagacatatt gccaattgtc gaaggttcac aagtaaacac aaaatacggg atcatccaaa
    74461 cagatcacgt cctattcatt gcttcaggag ctttccattt gtctaaacca agtgatctaa
    74521 ttccagaact tcaaggacgc ttcccgatac gggtagaatt agatgatttg acagctgaag
    74581 attttgtgaa gattctgacc gaaccaaata atgctttagt aaaacaatac gtcgcgttga
    74641 tcggtacaga aaatgtgtca gttacgttta caaaagaagc aattgaacgg atcgctcaaa
    74701 tcgctttcga tgtcaaccgt gacacagata atatcggcgc acgcagactg cataccattt
    74761 tggaaaaact actagaagat ctattatttg aagcaccaga tatgcaaatg ggagaaataa
    74821 cgatcacaga agcatatgtg gatgacaaac ttggctcaat tgcggcaaat gaagatttaa
    74881 gtcgctatat tttataaaaa atcggaggac gacaatgaca gacttgctaa cgaaaacccg
    74941 caagataaac acacttttgc agcaaaagaa tacttttgac atgcaagcag atttgcctta
    75001 cgataaaatg gctgtcacac taggtggtat tcttgacagc aacacctata tcataggaag
    75061 tgacggagta ttactaggtt ataatgaacg tcacgatgtg aacaatgaac gaatcaaaac
    75121 aatgtttgtt gagaaacaat ttcctgaatc ttatactgat acagtggcac agttgatgaa
    75181 aactgaagct aatatcccaa ttactagtga tattactgca tttccggtag agtcaagaga
    75241 gaaatatcca tttggtctga cgactgtcgt tccgatcttt ggagctggcg aacgtttggg
    75301 tacgattatc ttatctcgta tggaacaatc gtttgcagat gacgatctag tcttagcaga
    75361 atatggagct acggttgtcg gtatgcagat cctttatcag aagtctcgaa gcatcgaagc
    75421 agatgtacgg agtgcaacgg ctgtccaaat ggcaatcaac acattatctt atagcgaatt
    75481 gaaggctgta caagctatct ttgaagcatt ggatggagat gaaggtcgtc tgactgcttc
    75541 taatattgcc gatagtatcg gcatcactcg ttctgtgatc gtcaatgctt tacgaaaatt
    75601 ggaatcagct ggaatcattg aatctcgttc gttaggcatg aagggaactt atttaaaagt
    75661 attgaattcc cgttttaaag acgagttagc aaaacacagt tattagaagt aaggggactg
    75721 gggcagatag aaatgtttgt tccagccttc ccattttaaa aagtaagtcg aagttggagg
    75781 aatggcaatg actgttatta ttcaaaatga ccagctgatc gccgaaatcg ctgaacacgg
    75841 tgcggaattg atcagtctga aatcaaaaga acatggttta gagtatatct ggcaaggtga
    75901 tccagcgtat tggggcagac atgcaccggt tttattccct tttgttggac gtttaaaaga
    75961 tgatcagtat gtttatcaag ggaaaacata tcaaatgggg caacacggat ttgctcggga
    76021 tatggatttt gaagtcattg aacatggaca agaaatggct agtttcatct taaggagtac
    76081 gccggagaca ttagaaaagt atccatttga ttttgaatta gtgatcagct atgaattagg
    76141 aggagacggc atcacggttc attatcaagt ggcaaatacc ggtgaaaaag aaatgttctt
    76201 ttctatcggt ggacatcctg ctttcaatgt acctcttgaa gaaggattgt catttgaaga
    76261 tttctatctg gcattttctc cattgaaatc aagattacgc ctaccattga aaggcccatt
    76321 catcgatatg gatcaaaaga cgttaggtca gacaaatacg aatctggctt tgaatcgtga
    76381 actttttgag caagatgcct tggtttttga aacaaggggt ctaaatgcgt acgccattcg
    76441 aagtgaaaaa tcttcccata gcgtaacttt aagctataaa gatatgcctt atgtagggat
    76501 ctggtctcct tatccaaaag aagcgccttt cgtttgtatc gaaccgtggt gcggaatcgc
    76561 tgacacggtg aacagtagtg gaaacttgat ggaaaaagta gggatcaatc aactaggtgc
    76621 ccatgaaatc ttcaagacaa aatattccat cacagtgaag taagaggttg tgaccgagaa
    76681 gttagcaggg aacaccgttt accaaggtgt gtaaaaaagc tatcctgtaa gtaataaggg
    76741 agtgtgacat cgatttgtca cactcccgtt ttatctcttt ggaatgaaaa agtatcagga
    76801 ttttttcttg ctgctattca aaccaaacgg gattcgactt tcagtatggt ttctgatacg
    76861 cttgatattg tctttatgcc gataaaagat aaaggaagaa actaaaaagg caatgatcgt
    76921 cagtaaccaa ttgggcttag ccaaaatcac aggagctaca taaggaagga cgatcgtaga
    76981 taaagtgatc aatactgcac tgatcatact gctcaaactc accatacttg tacaaaataa
    77041 actaacgata aagatgatac aagaataaac aaaaaataca ggattaaatc ctaaaagcat
    77101 tcctgcactt gtcgcaacag cttttcctcc tttgaaccca gcaaaaatcg gaaatgtatg
    77161 gccaagaatg gcgcataccc caaaccaaag agggttaatc ccagaaaggt ggaagatcat
    77221 cggcaaacaa gtagccaatg taccttttaa aatatccata agcaggacaa caatcccggc
    77281 tgttttgcct aatacacgaa atgtattggt cgttccagta ttaccgctcc cgtgttgtcg
    77341 gatatctttt ttgaaaaaca atttgccaat ccagacaccg gaaggaatag aacctaaaag
    77401 atatgctaaa ataagtaata agatcaattt cattttttac cctctttcaa gttgaaaact
    77461 atcctttatt ttagcatgaa taggtaacag gaacaattga aaaaatgaaa gatacaagta
    77521 taatagatgg aataaaaaga aaagcaaggg gtggcagaaa tgtctgtaaa aaatccaaca
    77581 caagaaacga ttttcaacta tttggaagaa gcaaaaaata ttgccgtcgt aggcttgagc
    77641 aataaaccag aacgaacaag ttacatggtt gcacaggtat tgcaagacaa tggatataaa
    77701 atcataccag taaatccatt attggctggt gaagaaattc taggcgagaa agtctatgcg
    77761 aatttgacag aaatcgatga tccaatcgat atcgtagatg tatttagacg gagtgaattt
    77821 ttacctgaaa tagcaggtga ttttttgaaa actgatgcta aagtgttctg ggcacaatta
    77881 gggattgaaa acaaagaagc agctgagatg ctaagaaaag caggacgtac agatatcgta
    77941 atggatcgtt gtattaagat tgaacttggt aagttgaaag aaaaccaaaa ataaaaatga
    78001 ccacaaagaa gagatgatcg atagagggga atgtcgaaaa agcggttagg agataattaa
    78061 aattgatttc tcgtctaccc tctttttttt cacgaccttt tctagtatga taatcgtggt
    78121 gcggttttgc cgcaaagtat tattgaagga gcgtttgcct tggcaaaaaa agtaaacaac
    78181 gaatacaatg actcgtccat ccaagtattg gaaggattag aggcagttag aaaaagacca
    78241 gggatgtaca ttggatccac tgacagccga ggcttgcatc atttagtcta tgaaatcgtc
    78301 gataatgcag ttgacgaagc attatcaggt tatggcagtg agattgatgt gaccatccat
    78361 gaagacaata gtataactgt tgcagatagc ggacgtggga tgcctgtagg aatgcatgct
    78421 tccggaatac caacagttga agtgattttt actgtgctgc atgctggcgg aaagtttggt
    78481 caaggagggt acaaaacttc tggtggactg cacggtgtag gagccagtgt ggtaaatgcc
    78541 ttatcgaaat ggctaattgt aacgatcgtt agagatggcg tagagtatca gcagaaattt
    78601 aaaaacggcg gtaaacctga cggtactttg aaaaagatag gaaaaacaaa aaaagcaaac
    78661 gggacgactg tccatttttt accagatgat acaatattca gtacaacgaa attttcatat
    78721 gaaattttag ccgaacggtt aagagaatct gcctttcttc taaaaggcgt taaaatctct
    78781 ctttctgatc ttcgtggaga agaaccagtc aaggaaattt ttcattacga agaaggaatc
    78841 aaagaatttg ttgattatct gaatgaagaa aaagatacat tgacaccagt tgtttatttt
    78901 tcaggagaaa aagaaggtat cgaagtagag gtcgcttatc aatacaatga cggttattca
    78961 gaaaatgttt tatcttttgt caataacgtt cggacaaaag atgggggaac ccatgaagct
    79021 ggtatgaaag cggcgatgac gaaatcttat aatgaatatg cgagaaaagt tggtttgctg
    79081 aaagaacggg acaagaatct ggaaggtagt gatttcagag aagggttagc tgctgtactt
    79141 tctatccgtg tacctgaaaa tcttctgcaa tttgaagggc aaacaaaaga aaaacttggt
    79201 acgccagttg ctcgtaccgt agtagataac gtcatcagtg aacaaatggg cttttatttg
    79261 caggaaaata gcgaaatgag ccagatgttg gtccgtaaag caatcaaagc cagagaagcg
    79321 agagaagcag cacgtaaagc cagagaagaa agccgaaatg ggaagaagcg taaaaaaggc
    79381 gaatctcttc tttcaggaaa attgacaccc gcacaatccc gaaaccctaa gaaaaatgaa
    79441 ttgtatttag tcgaagggga ttctgctggc ggatctgcta aacaaggaag agacagaaag
    79501 ttccaagcaa ttctaccttt acgaggaaaa gtcatcaata ccgaaaaagc taaaatgcaa
    79561 gatatcttga aaaatgaaga aatcaatacg atgatctata cgattggtgc aggagtcggt
    79621 cctgagtttt ctatcgaaga ctgtaattac gacaaagtaa tcatcatgac cgatgcggat
    79681 acagacggcg cccatattca agtattactg cttactttct tttatcgtta tatgaaaccg
    79741 ctgattgaag ctgggaaagt atatatcgcg ttaccgcctt tatataaagt atccaaagga
    79801 caaggaaaga agcaagtcat tgaatatgcg tggacagatg acgaattggc agcaatgatt
    79861 aaaaaagtcg gcaaaggata tatgctgcag cgatataaag gacttggaga gatgaatgcg
    79921 gaacaattgt gggaaacaac gatggatccg acttcccgta cgttgatccg cgtccgtatc
    79981 gatgatgctg cgcaagctga acgtcgagta accacactga tgggtgacaa agtagaacct
    80041 cgaagaaaat ggatcgaaaa tcacgtgcaa tttacgctcg aagaagatgg cagtatctta
    80101 gataaaaaag aggatactga aatctctcct tcagtttcta atgatttatt agatgaagaa
    80161 cgagcagata aaaatgaaaa caatcagtta tttgaagtag aataggaggt cacctgatgg
    80221 aaaatcgaca agaagtacaa gaattaactt tagaagaagt gatgggtgac cgcttcggac
    80281 gttattccaa atatattatc caagaacgtg cactgcctga tatccgtgat ggattgaagc
    80341 cagtgcaacg ccggatatta tttgccatga acaaagatgg caatacgtat gacaaaggat
    80401 tccgtaaatc agcaaaatct gtcggaaata tcatggggaa ttatcatcct catggtgaca
    80461 tcagtattta tgaagcgatg gtccgcatga gccaagactg gaagttgcgg gaagtactga
    80521 tcgaaatgca cgggaataac gggagtatgg acggtgatcc acctgctgcg atgcgttata
    80581 ctgaagccag attatccaag ttaagtggag agatgctagc agatatcgag aaagaaacag
    80641 tcgatctcgt gtggaatttt gatgatacag aaaaagaacc aacagtcctt ccagctcgat
    80701 acccaaacct tctcgtcaat ggatcgacag gtatatctgc tggatatgca acagaaatcc
    80761 cgactcataa cttagctgaa gtgatcgatg gcacaatcta tatgatcgat catccacaag
    80821 cgagccttga aaaattgatg gaatatattc ctggacctga ctttccaaca ggagggatct
    80881 tgcaaggaaa agatgaaatc aaaaaagctt atgaaacagg acgaggaaaa gttattcttc
    80941 gttcgaaaac aaagatagag gcaatgaagg gtaataaaca gcaaatcgtg atcacagaaa
    81001 tcccatatga agtaaataaa gcgaccttgg tgaaaaaaat ggatgagatc cgtttgaata
    81061 agaaaatcga tggaatagct gaagtacgag atgaaagtga tagaacaggt ctgcaaattg
    81121 tcgtcgaatt gaaaaaagac gccaatgctc aaggaatttt gaattatttg ttcaaaaaca
    81181 ctgagctaca gatcaattac aatttcaata tggtcgctat cgaccatatg actccgcatc
    81241 aagttggact gaaagacatt ttaagaagtt atatcgagca tcgaaagcaa gtcataacaa
    81301 aacgtagtca attcgattta gccaaagctc aaaaaagaca gcatatcgta gaaggattga
    81361 tgaaagcttt atcgattctg gatgaagtaa tcgcaacgat tcgtgaaagt aaagacaaaa
    81421 aagacgccaa aaagaattta gtcgacgtct ttcaatttac agaagaacaa gcagaagcaa
    81481 ttgtcacatt gcaattatac cgtttgacga acacggatat cacagagctt caaaaggaat
    81541 ccgaaagctt gattgcgcaa atcacagagt tgaataaaat cctttcaaat gacaaagagc
    81601 ttttctctgt tatgaaaaaa gaattgcgag aagtgaaaaa aaattattct tccgctcgtc
    81661 taacgacaat cgaagacgaa atcgaagaaa tcaaaattga tacgcaagtc ttagtggcac
    81721 aagaagacgt aattgtcagt gtgacgcgag aaggctatgt gaagcgaaca agccttcgtt
    81781 cttatagtgc ttccaaacca gaagaaatcg ggatgcgaga aggcgactac ttgctatact
    81841 caggtgaatt gagtacattg gaccatgttc ttctgatcac aaataaagca aatgtgatct
    81901 accgccctgt ccatgaacta cccgatttga aatggaaaga tgctggtgag cacatttctc
    81961 aaacaatctc caatctgtct gtagacgaat cgattttagc tgttttccca tatcaaaaaa
    82021 ttcaagcaga gaaaacattt gtattcatca gcaagaatgg attgatcaag caaacacgaa
    82081 tgacagagtt cgaaccatgg cgcacctata agagccgtcc gctcagctgt atgaagctaa
    82141 aggcggaaga agatgaactg attgctgtgt atctgaaaga agatcagcca ggcttagatg
    82201 ttttcttagt aacgcatcaa ggaatgggtt tgcgctatcc tttaacagat gttcccgttg
    82261 ttggagcaaa ggcagctgga gtgaagtcta tgaatttgaa agatggagat aggattatca
    82321 acgggctgct tgtgttagca gaaggtgata cgccgatcgt aatcgttact cagcgaggag
    82381 ctgttaaacg catgcttgct caagaaatca gccaaacttc acgtgcgaag cggggagtga
    82441 cggtcatgcg tgagctgaaa aaaaatccgc accgtattat ctatatgtcg gaaggaagat
    82501 caaaagaaat aaccttgatg aatcaaaaag ggcagcagtt aacgattgac ccaactgatt
    82561 tccctatagg tgatcgaaca tcgaatggat catttgcgat ggatgagaaa aaaggtggag
    82621 aagtcatcga agttatcgat gctcctgaga ttttcatcga gtcgtaactg ttgtagtatt
    82681 ttaatgaaga gacgccatct gtattaatac aggtggcgtc tctttttcat tcatttttaa
    82741 taaatcctta tacgtttgca aaaaaagatg aaagtgttca caatgcttaa atgttggtat
    82801 gtcaacgttg atttaaatat atgtaaataa aacataaaaa ttgttaaata aaaaacaaga
    82861 aaacccttgc aaagataggg tgctgtgtta tactaatcac gtaaaaactg ttacataaaa
    82921 cagaaattta ggaggtccat acaatggagc aatggaatgg atttaaaggt accaaatgga
    82981 aagaaggcgt agacactaga gactttattc aaaataacta cactgaatat cgcggtgatg
    83041 acagcttttt ggaaggccca acagaagcaa cagaaaaatt atgggatgaa ttgcaaaagt
    83101 taaatgatat ccaattcgag cgtaacggtg tctatgatat ggacacagaa atcgtatcaa
    83161 ctattacttc acacgatgca ggctatttgg ataaagattt ggaaaaaatc gtcggtctac
    83221 agacagacaa acctttgaaa caagcattca tgccgttcgg cggaatcaat atggctaaca
    83281 atgcgctaaa aagcaatggc tatacaccaa acgatgaatt gacacatatt ttcactgact
    83341 ggcgcaaaac acataatcaa ggtgtatttg acgcttatac tgctgaaatg cgtgctgctc
    83401 gtaaaaacaa aatcattaca gggcttccag acgcttacgg tcgcggacgt atcatcggtg
    83461 actatcgtcg tgtggcattg tatggaatcg actatttgat ggaacaaaag aaaaaagatc
    83521 atgatatgac tggttatcat acaatgagtg aagaagtgat tcgtttacgt gaagaaatca
    83581 ctgaacaata tcgtgcatta gcgcaaatga aagaaatggc tgcgaaatat ggctatgata
    83641 tttctaaacc agctgcaaat gcaaaagaag ctgtccaatg gctatacttc ggatatttag
    83701 cagcaatcaa atcacaaaat ggtgctgcga tgtcaatcgg acgtatatca gcattccttg
    83761 atatctatat ccaacgtgat ttggataatg gagtaatcac agaaaaagaa gcacaagaat
    83821 tgatcgacca cttagtaatg aaattacgta tggtcaaatt cgcgcgtaca cctgaatata
    83881 accaattatt ctcaggattc cctatctggg ctactttatc cattgcaggt atgggattag
    83941 atggtcgttc attagttaca aagaatgatt tccgtctgct tcatacatta acaaatatgg
    84001 gaccttcacc agaaccaaac ttgactgttt tgtattctga acaccttcca gaaggattca
    84061 gaacatatgc tagcagaatc gctatcgaaa gttcttcgat tcaattcgaa aatgataatc
    84121 agcttcgtga acaatggggt tctgatgatt gtgcgattgc ttgctgtgta tcaggaacag
    84181 ttgtcggaaa agacatgcaa ttctttggcg ctcgtgctaa cttggctaaa acactattat
    84241 atgccatcaa tggcggagtc gatgaagtga ctggcgccca agttggacca gaatatcgtc
    84301 caatcacatc agaaatacta gactttgaag aagtgaaaca taacttcatg aatatgatgg
    84361 attggttagc agaactatat gtcaatacgt tgaacatcat tcattatatg catgataaat
    84421 attcttatga agcagctcag ctggctttga tggattctga cttgcaacgc acatttgcta
    84481 caggtattgc tgggatctct catgcggcag acagcttgtc agcgatcaaa tatgcaaaag
    84541 taaaagcaat ccgtaatgaa aaaggaatag cagttgattt tgaagtggat ggcgacttcc
    84601 ctaaatacgg aaatgatgat gaccgtgcag atgaattagt ttcatggatc ttggaatact
    84661 tcatgggaca aatcaaacgt cataaaactt accgcaactc aaaaccaaca acttcattat
    84721 tgacgattac ttcaaatgtt gtttacggta aaaatacagg aaatactcct gatggccgtg
    84781 aagctggaaa accacttgcg ccaggagcaa atccaagtta caatgcggaa caaagtgggt
    84841 tattagctag cctgaactca acagctaaaa tgaattacgg ctgtgcattg gatggaattt
    84901 caaataccca aacaatcaat ccttcaggtc ttggaaaaga acaagataca agaatcgaaa
    84961 acttgcgtaa tgtcttagat ggatacttct caaaaggagc ttatcatttg aatgtcaatg
    85021 tattcagtaa tgatttattg cgtgatgcaa tggaaaatcc aatgaaatat ccgaacttga
    85081 cgatccgggt atctggttat gcagttaaat tccgtgactt gacaagagaa caacaattag
    85141 atgtcttgat gagaacttca cacgacagaa tgtaaaaagt tcgtgaaaaa agtttttcga
    85201 cttagcgtcg aaaggacgtt gaatagccat cgcatcaaaa tcgaataatc agtatgctta
    85261 tttgaaagaa acgatttaca ggggcaggac ttgtatgttc cgtccctgta aattttacac
    85321 ataacgaagc ggcaattgcg aaaaattttg ttcagaaagc ttcgcttcga cagctaactt
    85381 gtacttaaaa tgaaatcaaa gaaaagatgt gaacttaaat ggaagaaaaa acaatcggct
    85441 atgttcattc tatcgaaact ttcggttcag tagacggacc tggactgcgc tttgtcgtgt
    85501 ttatgcaagg atgccgcatg cgttgccaat tttgtcacaa tccagatact tggaatattg
    85561 gcggagggaa agaatacaca gcagatgaac ttttagataa agcagaacgc ttcagacctt
    85621 actggggaga taaaggtggt attactgtta gtggtggaga acctctattg cagatcgatt
    85681 ttttgatcga actatttaaa aaagcaaaag agcggaagat gcatactaca ttggatacat
    85741 gcggaaaacc ttttacgtat gaagaacctt tcttttcacg ttttcaagaa ttgatgaagt
    85801 atacagattt actgttattt gacatcaaac acatcgataa tgaagagcat aaaaaactaa
    85861 cgcattggga caatgaaaat attttagaaa tggctcaata tttgtcaaaa atcaacaaac
    85921 cggtttggat tcgccatgta ctagttcctg agcgaagtga ttatgatgaa tatttgatcc
    85981 gactagacaa ctttattcaa accttgtcaa atgtagacag agtagagatt cttccttatc
    86041 atacgatggg aaaatacaag tgggaaacac ttggtttgaa atatccatta gaagggatcg
    86101 aaccacctac aaaagaacgt gtcgaaaatg ctaaacgatt gcttcataca gaagagtata
    86161 caaaatattt aacgcgatag gtcgcatcgg aaagaagata agtattgctt ctttccgttt
    86221 ttttgctatt attaaatagg agaaatcaca aaggagcgaa tgacatgtca aaaattttag
    86281 tttttggtca ccaaaatcca gatacagatg caatcggggc agccattgcg ttttcacact
    86341 tgcaaaatga attaggtaaa gaaacagaag cagttgctct aggtacacct aatgaagaaa
    86401 cacaatacgc attagaccac tttcagctat ctgcaccacg agtagtagag tcagctaaag
    86461 cagaaaatgc agatgtcatg cttgtggatc ataatgaatt ccaacaaagt atttcagata
    86521 tcagcgaagt aaatatttta gctgtagtag accaccatcg tattgcaaac tttgaaacag
    86581 ctaatccatt gtactatcgt gcagaaccag ttggatgtac aagtacgatt attttaaaac
    86641 tatataaaga aaaccaagta acaattccta aagaaatcgc aggaatcatg ctctcagcta
    86701 tcatttcaga tacattgttg ttcaaatcac caacatgtac gcctgaagat gaagcagctg
    86761 caaaagaact tgcagcaatc gctgaagtcg atctaaacac ttatggactt gaaatgttga
    86821 aagcaggaac aaatctaagt gataaatcag ctgcggttct tcttgatttg gatgcaaaaa
    86881 gtttcccaat gggcagttcc aacgttcgta tcgctcaagt caatacagtg gaccttaatg
    86941 aagtgatgga tcgacaagca gagctagaag cagcaatgga agatgaaatg aaagcaaatg
    87001 gctacgatct gttcgtatta gttgtcacaa acatcttaga cagtgattca gaattacttg
    87061 ttgctggtaa aggaaaagaa aaagtagaag aagctttcca aacaactttg accaacaacc
    87121 gtgctttgct taaaggtgtc gtttcacgta aaaaacaagt cgtacctcaa ttgacggctg
    87181 tatttagcta atcaaatggt agtatgaagc attcaaaatg aatggataag cgattgatat
    87241 aaaaagaaac atagaacata ctaaaggccg aagaaatgga ggagaataac agtcaattat
    87301 gactcttgtt cttcctcctt tttacgtaaa agccttttaa gtaaaataaa acggatagaa
    87361 aagagaacat atatgaacta ttattcaaaa aataaggaaa acttttatca aaaactgacc
    87421 gatgatccgc ttttttcttc attgaccgat tatctatatg aacatcgaga acaagaaacg
    87481 attctaagag aattgaaaaa agaattttcg caaaataaat tttcccattt tcttgattta
    87541 ttgatcgatg caggattaat caagagggaa gaacgaagat atcatttgaa ctttccaata
    87601 tttgatccta aagattactt gcaacaagcc acttcggctg ctgaaaccat agctgaacaa
    87661 ttgaagagac tctctgttga tgaacaaaaa ttagcgatgg gagaagttat ttgggcgtat
    87721 tgtttcgaag atgaacggaa agaagcgtat ttttatggag taagaaacag ccgagagaca
    87781 gaattgctga ggacaactgc aggaaatgag aagtaccgtt ttatcacact ttcctctata
    87841 gaacacttcc cgcttacttt ggctaattac ttttttgtac agaagaatca gcttcctgta
    87901 acgaaggcat tcaaagaatt agccgaactt attggagacg taaatgaatc gtactttttt
    87961 gatcaaatag aagttatcgt tgacaggata cgtaagaata aatataaaaa tcgccggcct
    88021 agtattttcc atcagtctct acttgtgacg aatactataa aggaagaaga atcattcaca
    88081 ttagaattac ctattgtcga aaaaaacaat tttgagattg aattaccaac attggatcct
    88141 tcattgacaa tggaggaaat ggcctttctt aaacgacaaa tcttttctga gctgagcaag
    88201 aaatttatac cgcatgcatt ttcttatatc aaagaatatg gaacagtttt agtgagtaaa
    88261 acctaagaga gcagtaagca aaaattatac tgactcttgc aggaggagcc tttctcttcc
    88321 tttatggtta aaagaattat ttgagtattt tgcctatcta ccacctatta gattcactac
    88381 atggtctgga aacaaaggaa tgcaaaaaaa tggtgagaaa tgtggaatga caaaggaagt
    88441 agtgataaga aaagttagat ttttaaataa tcaatattat gattcagtca agtatggcat
    88501 tttatgggaa gaactagctg attagattag gaagtccatt tgataatcca gaatcactaa
    88561 gaaacgaatg gcgagtcatt aataatttat ccaatcttgg gttattcttt tagattgaag
    88621 tgtttaaaac taaaaaagca tttatgaagt cgtaatagtc aggcttcaaa atgcttttta
    88681 ttttgtttgt tttttctttg ccacataaat acacctagac tgacgatgaa gagcatatcc
    88741 aacagatttc ctgcgacttg gatcgtcgaa gggttggtca gtggcattaa agcaaggaca
    88801 aaaaaggaaa acaagctgta aataaaacag gtgacaggaa ctaatcgaaa gaagagcgga
    88861 catttcaatt ggctaaaaat gagtgaaaaa ataaaataag taaaaatgga taaaatcacg
    88921 actaaaccat tcatgataaa gaagaaaagc gtattgtgat taaacgaata aaaaagaatt
    88981 gctaaaaaag aaaacgtttt aaatgcaatc aatacgcttg aagcttcgtt taacgtacca
    89041 ggatgtcgat gaaaaaaata ggcaccgaaa taaacagaaa acagtaagcc gcaaagagaa
    89101 gacaaaatca aaaaaaacgg attttcttcg gcttctgtga tatcagaatg atccaaaagc
    89161 aaccagccta atgcactaga aggcgcatgt gaggtcttaa ggtatgaata acatagaaaa
    89221 aataagatcg tcaaagttgc aaaaaaagag cctaaatgta aaaaattagt ggttctacgt
    89281 tgaatttcca taatatctcc aaattaaatt gataagtata taaagtataa taacaaattg
    89341 atgagtatat gtcgatgttt tttaatcaaa ataataaata aaaactgtct aacatttaaa
    89401 atttaattta gatattagac agtttttaag tttgtgagtt attttccttg tcgttttctc
    89461 acttttttag tccagaaacc acctgaaata tattttggct cataagaaat gataaaagca
    89521 cgctcttcga tttctttgat caggcgatac agttcacgtt cgctttttct aggagaaagt
    89581 atttcgagaa ctagccgctc accttccagc cccattgcag aagtctgtgt cacaccataa
    89641 ccgtgttctc tcagtatacc cggcaaactt ttatcttctg cgctatttgc tggcaaaatc
    89701 gttgtgacca tgatgtatcc taatgccaat ttgtcttcaa tcaaaattcc gacactgata
    89761 cctactgcgt aacctaacgc atagacaaat aaattcagtg gattatctaa acgatctaaa
    89821 acaagggata agccgacgat ataaatcgtg atctcgacca tactgacaag tggagcaatc
    89881 agtcgatagc ctttcattgt caacatgaaa cgtaacgtgt tcagagtgat ataagcaaaa
    89941 ttaatgagga agatcatccc caacattttt ggatcaatca tttgtaaaac tcctttcatt
    90001 tatataacaa caatatcaaa aaaaatcgta agtgaagaaa aattgaaact ttatacatac
    90061 aaaaaaatga taaaatgatt ataatagaat tataaagata ggttaggtga ttaaaatgaa
    90121 aacggtcaat cgtgtgtttt gtgccagcca cgtgctaagt ctccttctca gtatatactt
    90181 aggtgtctat tcgattcaac agccaattga ggtagagaac attgtttttc agatgtcgct
    90241 gacagatggt ttaattttaa gcgttttttt ctttttgata ctgtttattg gtaatatttt
    90301 cggtagcgtt gccagttttt tgaatttttc tatttatcca ctcctttcgc tagcattagg
    90361 agtggtggga ctttgttctc tgtgcttatt tcccgagcca ttcagtcctg catttatatt
    90421 atttggtaca ctgaacctgt tccagacaac tgttggagca tggttgctat ggcgatcagg
    90481 aaatttaatg aaaataggtg agtaacaatt gaatccaatt atattgaaac gttctgtcca
    90541 tcatattcca gtgttagaag ttgttctgaa agaattaaga gaggaacgta ttcccgttgt
    90601 catctattat cacggatggc agacaagtaa ggagttagtt ttgacgcaag gaagaaaaat
    90661 tgcgcagcag ggcattcgtg tgatcctgcc agatgcgatg aatcatgggg agcgcaaaca
    90721 gccggtttct tctattccat cctatacatt ttgggatagt atctacggaa atctgtttga
    90781 atttgacaca cttatcaagc atttagaaaa aagaggtctt ctacatgatc agcttgcagt
    90841 tggcggggtt tcgatggggg gaatgacaac atgcgccttg ttggctaaac atcctcatat
    90901 agtagcgggc gtgtgtttga tgggttcccc tgcacctttg gaatatggaa aagaaatcgt
    90961 aaggcgcgca tcggaatata attatgtcgt accaaaagac tattttgatt tgatttcatg
    91021 ggttaagagc tatgatttgt cattgcagcc ggaaaagtta gctggacgtc ctctgttctt
    91081 ttggcatgga caagctgacg aaaaaatccc ttatatacaa acggaacgat ttgtagaaaa
    91141 aaatcggcat aagtcttacg gaaaagaaat tgtttttcgt tcctcaaaaa cacagcgaca
    91201 tatggtacaa ataccactaa tggaagaagc ttccactttt ttagcaaaaa atttgataaa
    91261 aaagtataaa taattgaaac ggaaaatcac tcatgacgat agaggatttt ccgtttttta
    91321 ttttgggcta taataaactc tgttaggaaa atttggtata attgatagta agaatgacaa
    91381 catgttagaa aggaagagag catgcccact ttagcagatg ttgcaaaacg tgccaatgtt
    91441 tcaaaaatga cggtctctcg agtcatcaat catccggaac aggtgactga cgaattgaaa
    91501 gagttagttt tctcagcaat ggctgaactg gattaccgtc caaatattgc tgcaaaagca
    91561 cttgtcacga atcggtcgca gatcatcaaa ttatttatac tggaagaaat cgacacaact
    91621 gagccctatt acatgaattt gctgatggga atcgctaaac ggatcggtaa agcgcattat
    91681 tctctccaat tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg atacatcatc
    91741 acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt tggaaaaacc agttgtcttg
    91801 tttggggaaa attctgaagg atatgattat gtagacagcg ataatgaata tggtacagca
    91861 aaagcaacgc aatatgcctt agaaagagat tatcaaacga ttatttatat cggtatcgaa
    91921 gtggacgagc cttttgaaat ttcaagggaa actggttatt tatctgtgat gaaggaagtt
    91981 gatcttccta gtaaaatctt gcggtttcca aatcattcga cagaagtaga aagatatatg
    92041 acagaaaact gggcagattt tacagataaa acttgttttg tatgtagttc agatcggcta
    92101 gcactaggag cagtaagagg gattataaat gctggaggac acttgcccga agaatttggt
    92161 gtgattggat ttgatggggt atttttagac caaatcagtt cacctaaatt gacaacgatc
    92221 aagcaagata ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa aaaaatagaa
    92281 gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg aactagttat cagagagaca
    92341 acgaattaag tcttattact atatatatat ttatgaaatt catcgctatt ttcttaaaat
    92401 aatcagcgca ttgctactga aaacaatgaa tctgtgctat tattgagaaa agttccattt
    92461 ttgagaagga tggtttgaat gaaagaaatc agacgaaaac atttccgttt tcctgcgtac
    92521 gatgatgagt taggcgtaaa attaaccaac gacaagaagc gagtattatt tgacggacaa
    92581 gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac agatgaaaga atttcatttc
    92641 acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg caacaaatgg attgaagtca
    92701 gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat taccggatta taccggccat
    92761 taccaatcag aaatcacatc gacaggacga aaaaaacttt ttggtgattc ctctcgttct
    92821 gtcagacaaa gtgaaacaag gacagagtcc aagcatacta cacgaaacgc atctgttgac
    92881 acgaaggtgc gggagcgttc ttattttgtt cctaaatatg tacccgcatc gattatccca
    92941 gaccctagag aaagcaaaat ttctgaggat catttaatcg aatcgatgaa aaaaacggat
    93001 gactcttatc tgttgttcga tacagaaccg acgccgtatc aagaaaaaag aaatggtgag
    93061 ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg aaacaaagca gtcagaagaa
    93121 aaaaaatcga aaaaaagcca tggtatctta gaacgctcat tacaaggatt gatcgatgat
    93181 caaggaaact ctttgggaga aaatagctat tttaaataag tgatatacaa atttatattc
    93241 ggctacagtt ggaaaggaaa cgctgcggcg tttccttatt ttatactcta tccgaaagtt
    93301 tatatcgtat cctcaaattt ttttgagttt aagcgaattt tttctcccaa taagtaagaa
    93361 aataatggca aaataaaaaa acaaactatt ttcgcagatc gtttgcaaaa atagtttatt
    93421 tttttataaa gaagatttta ctgaataata tcattttagt ttacataatg atatttctgt
    93481 aaattaaata aagtagtgaa aaataagaat cagtgctcta actgttcaag aggatcaagt
    93541 ggttcaaatg tttctgtagt ctgttttgct tccaattggg gcaaaacaga tccaatttcc
    93601 agatacttca ctttattcaa tgtataagat agaatagaac agctaagtaa tgtatacaga
    93661 aaatgtgtga gatccaaata aagcaaggag acaactaaaa taccaaaagt aatcaagaaa
    93721 agacttttgc caatcttaat atgaaaatat ttttcaatgg cttgataaat gatatctgtt
    93781 ccgcctgtgc ttccgccgaa tcgaaagacg agaccgcaac caatgccaga aagaatacct
    93841 gtcatcaaac tattgatcag tagactatct acagcaaaat gtatatgaag gctctcccat
    93901 aaatgtaaaa agaaaactag cgcaaatgtt ccgacgatcg tccggagaaa agcgtgtttt
    93961 ccaaaaagaa acaatgagat aagcaataaa ggtaaattaa aaagcagtga agtgacagaa
    94021 ggagcgaatc caaaaagatt atgcaaaagc aaagcaatac cagtaacccc accatctgcc
    94081 agcttagatg gaatattgat tcctgtcaca gctaatccgt agattcctgc gcctaaaatg
    94141 attccgcaca attctgtaaa taaatttaat tttttcattt ctagcctctt ttcttttgaa
    94201 aattttcagc aacttcttta ttatactgac ttatactctt acttcaagag gaaaatgaaa
    94261 aaaaattatt tttggctgtt tctttttatt tatcaaataa ggagtggaaa aatgcgagca
    94321 gcagacttat tgacaattct tacaaaatca cccaaaaaaa cagagtatca aggagttttt
    94381 ttggctcaag aacaaaaact ggtgcctgtg acaatggtcc agataaataa agcaaatcaa
    94441 tttatctttt tatttgaaca cggcaaagca ccacttgcta tgaaagacat ctttcttttt
    94501 ttgatgaaga accgtgagaa agctgtttat tatcagtcaa aagaagaact cattcctctt
    94561 tatggagtga aagagataga aaataaagtg gtgatataag taaaaataag aaaacaagac
    94621 gtttatgatg tcaaaacaac cattttttac atttttggca aaaaagtgtc atgatacagt
    94681 ataatattcg ttgaacaggc caataaaggt gttctccccc acgagatttc tactttattg
    94741 gctgtttctt tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc tgattccaag
    94801 tgacaatttc gtatagattt ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg
    94861 atccgacttt gttgcttgga ttcttttttt gtcagcatat ttagaggtat ttttttcttt
    94921 tttttgttcg tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg
    94981 tttatactta aagaataaca gaaaggaagg tcgcatgaat aattttatac aaacattaag
    95041 tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat ctgtccattt ctctcatttc
    95101 tttgctgatt gcagcactga ttgctattcc tttagcgatt tgggcagcca atcataaaaa
    95161 aatggcagaa gtcttattgc agatcacggg tgtgctccaa acgattcctt cacttgcctt
    95221 attaggttta cttatcccat ttgtagggat cggaactgtt ccagcattga ttgcacttgt
    95281 tatttacgct ttgcttccga ttttccaaaa cacgtatatt ggtctagctg aaattgaccc
    95341 gtctattgaa gaagcagcgg tagcatttgg tatgtccaga atgagacgct tgctgaaagt
    95401 agagcttccc atcgcattac ctgtcattat ctcaggtatc cgaactgcct tagttttaat
    95461 catcggtacg gctactttgg cagcattgat cggagcaggt ggattaggga catttatttt
    95521 gttaggtatt gaccgaaata cacctgtttt gacattgatt ggtgcgatca gttctgcctt
    95581 gcttgcaatt atattcagcg gactgattcg ctggttgcag cataagaaac ctcgatattc
    95641 catcattact ttagcagcag tggtgtgttt atttggcggc gtttctttat atcagtctgc
    95701 cttgtttaaa aatgaaacca tcacgattgc tggaaaatta ggagcggaac ctgaaatcct
    95761 gatccaaatg tataaagaat tgattgaaga caacacaaaa acagaagttg aattaaaacc
    95821 gaattttggc aagacgagtt ttttgttcaa tgcactggat caaggtcaaa tcgatatcta
    95881 tcctgagttc acaggtactg tcctagaaag cttggtgaag gtacctgaag caatgaaaag
    95941 tgctgatttg acaaaagaaa aaacatatga cgaagcagaa gaattattga ataagcaatt
    96001 cgatatgaca ttattaaaac caatggctta ccaaaatacg tatgccttgg cagtcaagag
    96061 atcttttgcg aacgaacatc aacttaaaaa gatttccgat ttggcagcta tctctgatca
    96121 gatcaaagcg ggattcacac tggaatttat tgatcgttca gatggatata aaggaatcca
    96181 agaagcatat ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt tgcgttacca
    96241 agcaatcaat aatggagatg tcaatattgt ggatgcctat tccacagata gccagctcaa
    96301 ggagtacgat ctagtgactc tagaagatga tcgagggtta ttcccagctt atcaaggcgc
    96361 accgctgatg aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag cactgaataa
    96421 attaagcgga aaaataacag aggatcaaat gattgatatg aattaccaag tcaatgtcga
    96481 aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa aatgaaggat tagtaaagga
    96541 ggaagatcag tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag
    96601 tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag aaatctttgt tttggttggt
    96661 ccttccggta gtggaaaaac aactactttg aaaatgatca atggattatc taaaccatca
    96721 gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg
    96781 tggaatatgg gatatgtgct gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa
    96841 aatattgaag tcatccctga aatgttaggt tgggaaaagc agaaaagagc agaccgtgta
    96901 gatgaacttc ttcaaaaagt tggcttatct cctgatattt atcgagatcg tatgcctaga
    96961 gagttgtctg gaggagaaca gcaacggatc gggatcatcc gagctattgc tgcaagccca
    97021 gatgtgattt tgatggacga gccatttagc gcattagatc cgatttcaag aaactcatta
    97081 caagaattgg ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt cgttacgcac
    97141 aatatggaag aagcaatcaa gctaggtgat cgtattgcat ttatgaagga tggagaaatt
    97201 atccaatgtg acacaccaga gcagttattg atgaatccta aaaacgacta cgttcggcat
    97261 ttctttgatg aaccgaaaca gacgaaagag tggcgagtag aagatttagt cgtcaatgga
    97321 tatttcttaa atgaaatacc agaaaatgct cgtcaacaag tttgctttga tacgcctatg
    97381 aaagaagtat attcgcttct ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga
    97441 agtattgggt cgctgacaag taaagaaatc tttgcttttt taagtcgaga ggaggaagga
    97501 aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta
    97561 tggattgaaa gaagcaggta aaacagtact agtggtcgaa gaagatctat ggggcgggac
    97621 ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa
    97681 tcgggtaaaa caattgagtg gaaaaggttt taacgagatc ccaactgcca actgggaaga
    97741 acttcaaaaa ttcaaacgaa catttacgga tccagtccca gaaagccgaa aaaaacaatt
    97801 agcagaagct gaaatcgatc atctttcagg aaccgctcgt tttttggatg attcatctat
    97861 tgaagtcaac gaagaagtct tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc
    97921 tacgatctta ccagtggagg gaaaggaata cttgaaaact agtgcagact ttttatcatt
    97981 gcctgtactg cctaaagaaa tcatttttat cggtggtggt tatattgctt ttgagctagc
    98041 tacgattgcc aatgctgcag ggagcaaagt aaccattgtc catcataatc aacggccatt
    98101 aaaagaattt gaggcgtctt tggtagagga agccgtccat caaatggaag catctggcat
    98161 tcagtttgct tttggtgttg aaacccaaaa gatcatttca gaaggtactc gctatcgttt
    98221 agtcggaaaa gagacggaat tagtagcaga tatgattttt tgtgcgactg ggagacagcc
    98281 gaatacagaa tctttagcac ttgagcaagc gaatatcgtc tttgataaac acggaattgc
    98341 agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag atattgtttc
    98401 aaggaaaacc ccaaaattga caccagtcgc cacttttgaa ggaaattatg tagctaagcg
    98461 gataacggat gcaacaagcg aacctatcaa atatccgatt atacctacga tcgtctacgc
    98521 cagtcctaaa cttgctgaag taggagttac aaaaagtcac gcctcttcgt cagatcaagt
    98581 ggtagagatg gatcttacca gttggttcac ctatcatcgt gtcaatgaac cagttgctaa
    98641 ggctgaattg acctttgacc agcaaaatta tttgattggt gctgcagtga ttagcgagca
    98701 agcagacgaa ttgatcgatg atttgacact cgtcatcaat caaaaattga ctaaaaaaga
    98761 gttggatagc tatatcatgg ggtatccgac attggcaagt gatctctcct atttattgaa
    98821 ataatcagca tgataactaa agcgcttgtt gctttttcct tcttgtgcta cactttaaaa
    98881 aatgaatacc tgtttctggg gatgtcgtag acgacatgag agtaagttgt gaacttagac
    98941 ccattgaacc tgtcagttaa tactggtgaa gggagaaaca tagcagacgc ttgaagcggg
    99001 ttactatgtc gtgcccattt caagcgtttt ttgttattca tattagaaga aaggaggtaa
    99061 caaaatgaag aaaaatcttt atccagcagt cagtatggca ttcattttaa ttatttggca
    99121 gattgttgtc atagtttgga aaatacctgc ctttatttta cccggacctg ctgctgtatt
    99181 ttctgcatta gcggcagatg cacaagtatt actttcccat tctgtcacga cattaaatga
    99241 agcagtgctt ggtttggtaa tagctagcgt tctttctttt attactgcct tggcaatgga
    99301 ttattggaag tttttagaaa tgtcttttta tccgttacta gtgatttcac aaatgttgcc
    99361 aatcatggtg ttagggccat tacttaccct atggtttggt ttcggtatgc ttccaaaagt
    99421 gatccttgtt gtcttgataa gctattttcc gatcgtagtt tctttttcgg atacattgag
    99481 gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg ggggcagata cagggaagat
    99541 ttatcgaata tataaaatac cagcgggttt atccggcttc ttttccggtt taaaagtggc
    99601 tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt
    99661 agggtattac atgatacgcg taaaaaacag ctaccagatc gataaagttt ttgctgcgat
    99721 catttgtgtc attctattga gtttattgct gaatggcgct tgttcattat tgaaaaaagg
    99781 gtattatcgt atgctttata ttttttaaga gaaggggatc aaatgaaaaa attatggctt
    99841 ttattaccgc tactgttgct tttatccgct tgtggcactg caaaagaaac aagtagcaaa
    99901 caagaaataa aggatttgaa aaaagtgaca cttgttttgg attatgtacc gaatactaat
    99961 catactggca tctacctagc aaaagaaaaa ggctattata aagaggctgg tttaaatgtc
   100021 caaatcatcg aaccaggaga taattcgaca agtattggtc tagtaggtgc ggacaaagca
   100081 caatttggtg taagttatca agaagatgtc acttatgctc acgcagatgg acagaatata
   100141 ccagtcaaag caattgctac agtgatcaaa cataatactt ctggattcgc caccctatca
   100201 gacagtaaca tccattcacc aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt
   100261 ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag acggcgggga cttttcaaaa
   100321 ctgacaatgg taggaagcaa tggagaaggg ccagaaagct taggaaaatc ctcagatatc
   100381 caatggtact ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat tgaactaaac
   100441 tatatcccat tgaaagaatt agatgaacgg ttagattact atactccggt aattattacg
   100501 aatgatcaac tgatcaaaag tgatccagaa ttggtacagt cttttatgga tgccacgaaa
   100561 aaaggctatc aggaagctat taaggatcca aatgacagtg ctaaactgtt gcaaaaatat
   100621 gcaaaagaaa atgatcggac gtttttagaa gaatcacaag cattcttgtc aaaaaattat
   100681 acggatgatc ctgagaattg gggattgatg gaagaaaaag tttggcgcaa ttatacagct
   100741 tttatgcagg aaaacggatt gatcaagcaa gatgtacctt cgcaagaact attcactaat
   100801 cagtttataa aataggagtg atagatatgt caatccaaat cagccacttg aatgtagtca
   100861 ttgaaaacct gaaaatatta gaagacatcc atttcactat ccctaaagat acctttacat
   100921 cgattgtcgc gccaagcgga gccggtaagt caacattgct gaaaacttta acaggagtcc
   100981 agcctatgac ttctggaacg atcaaagtag acgatcaaaa agtaactggt ctccatacag
   101041 catttagtta tatgccgcaa gaagatatgt tacttccttg gctgagcgtc tatcaaaacg
   101101 ttacgttata tcaaaaaatc aatcatctaa caatcaatga agaacaagta agggattatc
   101161 tggatatctt tgggttaaac ggctatgaac attttttgcc ggaacaatta tctggcggga
   101221 tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg
   101281 atgaaccatt tggagcgtta gatgctatga ccagagggca gatgcaagac tggatgttga
   101341 agctgcctaa aaaagcaaag cggactactt tacttgtgac gcatgatatc gaagaagcaa
   101401 tttatttatc agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga
   101461 ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact gcagcaatca gagttgaagc
   101521 aaacgattta tcagttattg gcaggtgaat tagatgttaa gtgatgctca ttgtcattta
   101581 gatgggagtc agagtttagc actccttcag caggaacaca cgattttgac gatcatcaat
   101641 tgtgattcgc cagaagaatg gaaggaaaac agacagcttg cagcaagtaa gactcaagcc
   101701 ttaagttacg gtatccatcc ttggaaagca gatagctata cgtttgaaca agttgagcct
   101761 tttttgaaaa aagcaaggat catcggtgaa atagggttag ataatatttg gacgaatgta
   101821 ccgatgacta cacaaaaaaa agtatttgag cgacagcttg cttttgcagc cataaatgag
   101881 aaacctgttg tactgcacac aaaaggatgc gaaaaagaga tcttaaatta tatccaagct
   101941 taccctaatc actatttgat ccattggtat tcttctttgg actatcaaca agactatatt
   102001 gatttaggct gttatttcag cattggtctt gatttgaaaa agaatccagc agtctggcaa
   102061 ctagctaaag cagtcccaat cgaccactta cttattgaaa cggatggact acatgcagca
   102121 gagtgggctt taggggggat tttttcagaa aaagattatg tctcactgct tgaagaacag
   102181 ttaaagacaa tcgctgagat caaagagcta tcagttgaag aagttaaaaa aaatacttat
   102241 caaaacttgg aacgtttcat tagattaggt aaataaaaaa gagatgtgac acaactagtg
   102301 tcgcacctct ttttggcttt ctttgtataa aagtaaactg tttgattaat aaatgaatct
   102361 tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa
   102421 gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa
   102481 gaagaatcta attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt
   102541 gattcttttt gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt agcagcagtt
   102601 tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt ttccgtttga
   102661 accgtttctt gtacaacagg ttgttcagct tggactggtg cttcttgaac ggctgcttga
   102721 ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagta
//
LOCUS       DACCCR010000002        69092 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCCR010000002 DACCCR010000000
VERSION     DACCCR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.002"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(2..118)
                     /locus_tag="IVK93_000095"
     CDS             complement(2..118)
                     /locus_tag="IVK93_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP6385420.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(191..1147)
                     /locus_tag="IVK93_000096"
     CDS             complement(191..1147)
                     /locus_tag="IVK93_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6385421.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1318..1686
                     /locus_tag="IVK93_000097"
     CDS             1318..1686
                     /locus_tag="IVK93_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP6385422.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1856..2287
                     /gene="mraZ"
                     /locus_tag="IVK93_000098"
     CDS             1856..2287
                     /gene="mraZ"
                     /locus_tag="IVK93_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP6385423.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            2308..3267
                     /gene="rsmH"
                     /locus_tag="IVK93_000099"
     CDS             2308..3267
                     /gene="rsmH"
                     /locus_tag="IVK93_000099"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP6385424.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            3272..3673
                     /gene="ftsL"
                     /locus_tag="IVK93_000100"
     CDS             3272..3673
                     /gene="ftsL"
                     /locus_tag="IVK93_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP6385425.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            3674..5866
                     /locus_tag="IVK93_000101"
     CDS             3674..5866
                     /locus_tag="IVK93_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6385426.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5892..6854
                     /locus_tag="IVK93_000102"
     CDS             5892..6854
                     /locus_tag="IVK93_000102"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP6385427.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6868..8229
                     /locus_tag="IVK93_000103"
     CDS             6868..8229
                     /locus_tag="IVK93_000103"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP6385428.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            8255..9343
                     /gene="murG"
                     /locus_tag="IVK93_000104"
     CDS             8255..9343
                     /gene="murG"
                     /locus_tag="IVK93_000104"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP6385429.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9358..10578
                     /locus_tag="IVK93_000105"
     CDS             9358..10578
                     /locus_tag="IVK93_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP6385430.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10734..12059
                     /gene="ftsA"
                     /locus_tag="IVK93_000106"
     CDS             10734..12059
                     /gene="ftsA"
                     /locus_tag="IVK93_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP6385431.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            12082..13323
                     /gene="ftsZ"
                     /locus_tag="IVK93_000107"
     CDS             12082..13323
                     /gene="ftsZ"
                     /locus_tag="IVK93_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP6385432.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            13340..14017
                     /locus_tag="IVK93_000108"
     CDS             13340..14017
                     /locus_tag="IVK93_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP6385433.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            14031..14630
                     /locus_tag="IVK93_000109"
     CDS             14031..14630
                     /locus_tag="IVK93_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP6385434.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14645..14932
                     /locus_tag="IVK93_000110"
     CDS             14645..14932
                     /locus_tag="IVK93_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP6385435.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14981..15763
                     /locus_tag="IVK93_000111"
     CDS             14981..15763
                     /locus_tag="IVK93_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP6385436.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15784..16491
                     /locus_tag="IVK93_000112"
     CDS             15784..16491
                     /locus_tag="IVK93_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP6385437.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16753..19551
                     /gene="ileS"
                     /locus_tag="IVK93_000113"
     CDS             16753..19551
                     /gene="ileS"
                     /locus_tag="IVK93_000113"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP6385438.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19600..21126)
                     /locus_tag="IVK93_000114"
     CDS             complement(19600..21126)
                     /locus_tag="IVK93_000114"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP6385439.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(21141..21788)
                     /locus_tag="IVK93_000115"
     CDS             complement(21141..21788)
                     /locus_tag="IVK93_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP6385440.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21972..22301)
                     /locus_tag="IVK93_000116"
     CDS             complement(21972..22301)
                     /locus_tag="IVK93_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP6385441.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22478..23206
                     /gene="sfsA"
                     /locus_tag="IVK93_000117"
     CDS             22478..23206
                     /gene="sfsA"
                     /locus_tag="IVK93_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP6385442.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            23222..24235
                     /locus_tag="IVK93_000118"
     CDS             23222..24235
                     /locus_tag="IVK93_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6385443.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            24235..25512
                     /locus_tag="IVK93_000119"
     CDS             24235..25512
                     /locus_tag="IVK93_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6385444.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25575..28277
                     /locus_tag="IVK93_000120"
     CDS             25575..28277
                     /locus_tag="IVK93_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6385445.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28429..28746
                     /locus_tag="IVK93_000121"
     CDS             28429..28746
                     /locus_tag="IVK93_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6385446.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28776..29096
                     /locus_tag="IVK93_000122"
     CDS             28776..29096
                     /locus_tag="IVK93_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6385447.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            29204..30664
                     /locus_tag="IVK93_000123"
     CDS             29204..30664
                     /locus_tag="IVK93_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6385448.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30732..30953
                     /locus_tag="IVK93_000124"
     CDS             30732..30953
                     /locus_tag="IVK93_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385449.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30984..31166
                     /locus_tag="IVK93_000125"
     CDS             30984..31166
                     /locus_tag="IVK93_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP6385450.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            31166..31579
                     /locus_tag="IVK93_000126"
     CDS             31166..31579
                     /locus_tag="IVK93_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP6385451.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31702..32883
                     /locus_tag="IVK93_000127"
     CDS             31702..32883
                     /locus_tag="IVK93_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6385452.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            33272..33345
                     /locus_tag="IVK93_000128"
     tRNA            33272..33345
                     /locus_tag="IVK93_000128"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33306..33308,aa:Arg,seq:cct)
     gene            complement(33414..34553)
                     /locus_tag="IVK93_000129"
     CDS             complement(33414..34553)
                     /locus_tag="IVK93_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6385453.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(34852..35487)
                     /locus_tag="IVK93_000130"
     CDS             complement(34852..35487)
                     /locus_tag="IVK93_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385454.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(35600..36235)
                     /locus_tag="IVK93_000131"
     CDS             complement(35600..36235)
                     /locus_tag="IVK93_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP6385455.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(36269..36730)
                     /locus_tag="IVK93_000132"
     CDS             complement(36269..36730)
                     /locus_tag="IVK93_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP6385456.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(36860..37291)
                     /locus_tag="IVK93_000133"
     CDS             complement(36860..37291)
                     /locus_tag="IVK93_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6385457.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(37309..37629)
                     /locus_tag="IVK93_000134"
     CDS             complement(37309..37629)
                     /locus_tag="IVK93_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6385458.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            37928..38704
                     /locus_tag="IVK93_000135"
     CDS             37928..38704
                     /locus_tag="IVK93_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP6385459.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            38719..38922
                     /locus_tag="IVK93_000136"
     CDS             38719..38922
                     /locus_tag="IVK93_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6385460.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            38938..39276
                     /locus_tag="IVK93_000137"
     CDS             38938..39276
                     /locus_tag="IVK93_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP6385461.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            39263..39442
                     /locus_tag="IVK93_000138"
     CDS             39263..39442
                     /locus_tag="IVK93_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385462.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(39485..39955)
                     /locus_tag="IVK93_000139"
     CDS             complement(39485..39955)
                     /locus_tag="IVK93_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385463.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            40042..40740
                     /locus_tag="IVK93_000140"
     CDS             40042..40740
                     /locus_tag="IVK93_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP6385464.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            40918..41259
                     /locus_tag="IVK93_000141"
     CDS             40918..41259
                     /locus_tag="IVK93_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385465.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            41252..41923
                     /locus_tag="IVK93_000142"
     CDS             41252..41923
                     /locus_tag="IVK93_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP6385466.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            41929..42615
                     /locus_tag="IVK93_000143"
     CDS             41929..42615
                     /locus_tag="IVK93_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385467.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            42618..43367
                     /locus_tag="IVK93_000144"
     CDS             42618..43367
                     /locus_tag="IVK93_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6385468.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            43379..43648
                     /locus_tag="IVK93_000145"
     CDS             43379..43648
                     /locus_tag="IVK93_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385469.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            43810..44112
                     /locus_tag="IVK93_000146"
     CDS             43810..44112
                     /locus_tag="IVK93_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385470.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            44109..44270
                     /locus_tag="IVK93_000147"
     CDS             44109..44270
                     /locus_tag="IVK93_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6385471.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            44267..44572
                     /locus_tag="IVK93_000148"
     CDS             44267..44572
                     /locus_tag="IVK93_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385472.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            44572..44928
                     /locus_tag="IVK93_000149"
     CDS             44572..44928
                     /locus_tag="IVK93_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP6385473.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            44888..45133
                     /locus_tag="IVK93_000150"
     CDS             44888..45133
                     /locus_tag="IVK93_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385474.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            45130..45549
                     /locus_tag="IVK93_000151"
     CDS             45130..45549
                     /locus_tag="IVK93_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP6385475.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            45546..46103
                     /locus_tag="IVK93_000152"
     CDS             45546..46103
                     /locus_tag="IVK93_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP6385476.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            46100..46396
                     /locus_tag="IVK93_000153"
     CDS             46100..46396
                     /locus_tag="IVK93_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385477.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            46473..46886
                     /locus_tag="IVK93_000154"
     CDS             46473..46886
                     /locus_tag="IVK93_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP6385478.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(47344..47619)
                     /locus_tag="IVK93_000155"
     CDS             complement(47344..47619)
                     /locus_tag="IVK93_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385479.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            48073..48279
                     /locus_tag="IVK93_000156"
     CDS             48073..48279
                     /locus_tag="IVK93_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385480.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            48475..48642
                     /locus_tag="IVK93_000157"
     CDS             48475..48642
                     /locus_tag="IVK93_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6385481.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            48668..49012
                     /locus_tag="IVK93_000158"
     CDS             48668..49012
                     /locus_tag="IVK93_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6385482.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            49017..49298
                     /locus_tag="IVK93_000159"
     CDS             49017..49298
                     /locus_tag="IVK93_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385483.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            49401..49715
                     /locus_tag="IVK93_000160"
     CDS             49401..49715
                     /locus_tag="IVK93_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP6385484.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            49693..51387
                     /locus_tag="IVK93_000161"
     CDS             49693..51387
                     /locus_tag="IVK93_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP6385485.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            51407..52585
                     /locus_tag="IVK93_000162"
     CDS             51407..52585
                     /locus_tag="IVK93_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6385486.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            52548..53234
                     /locus_tag="IVK93_000163"
     CDS             52548..53234
                     /locus_tag="IVK93_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP6385487.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            53234..54394
                     /locus_tag="IVK93_000164"
     CDS             53234..54394
                     /locus_tag="IVK93_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6385488.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            54404..55279
                     /locus_tag="IVK93_000165"
     CDS             54404..55279
                     /locus_tag="IVK93_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385489.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            55276..55587
                     /locus_tag="IVK93_000166"
     CDS             55276..55587
                     /locus_tag="IVK93_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385490.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            55577..55930
                     /locus_tag="IVK93_000167"
     CDS             55577..55930
                     /locus_tag="IVK93_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP6385491.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            55920..56321
                     /locus_tag="IVK93_000168"
     CDS             55920..56321
                     /locus_tag="IVK93_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385492.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            56314..56718
                     /locus_tag="IVK93_000169"
     CDS             56314..56718
                     /locus_tag="IVK93_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385493.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            56730..57338
                     /locus_tag="IVK93_000170"
     CDS             56730..57338
                     /locus_tag="IVK93_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6385494.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            57358..57720
                     /locus_tag="IVK93_000171"
     CDS             57358..57720
                     /locus_tag="IVK93_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385495.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            57723..57905
                     /locus_tag="IVK93_000172"
     CDS             57723..57905
                     /locus_tag="IVK93_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385496.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            57922..61353
                     /locus_tag="IVK93_000173"
     CDS             57922..61353
                     /locus_tag="IVK93_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP6385497.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            61404..62141
                     /locus_tag="IVK93_000174"
     CDS             61404..62141
                     /locus_tag="IVK93_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6385498.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            62151..64442
                     /locus_tag="IVK93_000175"
     CDS             62151..64442
                     /locus_tag="IVK93_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385499.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            64466..66592
                     /locus_tag="IVK93_000176"
     CDS             64466..66592
                     /locus_tag="IVK93_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385500.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            66755..67201
                     /locus_tag="IVK93_000177"
     CDS             66755..67201
                     /locus_tag="IVK93_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385501.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            67203..67340
                     /locus_tag="IVK93_000178"
     CDS             67203..67340
                     /locus_tag="IVK93_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP6385502.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            67378..67671
                     /locus_tag="IVK93_000179"
     CDS             67378..67671
                     /locus_tag="IVK93_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385503.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            67668..67892
                     /locus_tag="IVK93_000180"
     CDS             67668..67892
                     /locus_tag="IVK93_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP6385504.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            67889..68914
                     /locus_tag="IVK93_000181"
     CDS             67889..68914
                     /locus_tag="IVK93_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6385505.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        23654 a        11191 c        14276 g        19971 t
ORIGIN      
        1 tttacatgac actcattaca gcagtcaata caacagatcc aatcgttaaa atcagcattg
       61 tccaaatgac aaattttgtt actttagcaa aagtacttgt ttgttttttt tcacgcattg
      121 ttttcaacct cgtcattaat tagttgattc aagtgtacaa tcttttttcc atttccacaa
      181 gagggctttt ttataaaaac ttcccttttt ttaaataata ggtagttacg atacaaataa
      241 ataatgttaa tagaactagc catataaagg catcatccct ttcagcaata ggtaacttaa
      301 tgttcattcc atagatactg ccgacgattg tcggtatagt taaaataatc gttaaagaag
      361 ttaaaatttt cataatatta ttcagattat tcgatacgat tgctgaaaaa gtctgattca
      421 tttgttctac tagttttaat tgaatgcgag aagtagtcat tgcctgctct gtttccacca
      481 atacatcacg taaatgattt ttcaaagcat gattttcttg gaaaagttta ttttgcgcta
      541 gttctttcaa tgcatctaaa ttagcagaag tcgcttctct gaaataaacg aggctttttt
      601 ggatatccat gatttgataa agttgtttgt tttcagtgga tacttgaagt ttggcttcca
      661 aagtatcgca ttgagattga agcgtagcta aataactgtt gtaagagagt gtgacctgcc
      721 aaatgaattg aaagaagata ttgagattag gcgaaatatc actttttgga aaggtctggc
      781 taaagacttt ctttaaaaac agtggttcat gattcgtaat tgtgataagc ttctttttgg
      841 gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa ataaccgcta ggacttgttt
      901 tagcaaaagg atattgtaac aataataacg ctggctttgt caactttgat tgattgaatt
      961 cttctgtacg ggaattttct gcatcgtcca atatggaagt gatgtaatct gacggaattt
     1021 caaattgatg tgtcagctga tgaatctctt cttgattagg acgttctaca caataccaga
     1081 tcgtttggtc agtttccttt ccggtattac gcagtttctc attatctaat gtaaaatatt
     1141 tgatcatgat tttcctcctg ttgcctgttt cttttattat aacggatagg gaattggaat
     1201 acatggaatg tgtttgtaag atatagaata taactgcata aatttaaata aaatgaaaca
     1261 gttgtgtaaa gtatatacag tatacttgaa gcaacgataa aaaaggagtt tacgttcatg
     1321 ggcacattca caccacttat cttgttttgg tatatttacc cggtaatcgt tttgtttggt
     1381 tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt ttaaaataaa agcccctgat
     1441 ttggcgattc cttttttgtg gggcggtatg catgcattgt ctagaaatac agggacaatt
     1501 tctatcttac cgtttttctt tatttctgtt ttgctaatgg gaatttgtat tgctgttttc
     1561 caagcatatt attatgaaga aattatttat ccgcgttatt tcaagatgac ttggcgtttg
     1621 gtttttctgt tgacaatcgt cttgtacgct gtcttgatca ttgtgaacat actttcttac
     1681 ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa ggctttttgc agcttgattt
     1741 ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat ttcccagaaa aggtggtaga
     1801 aagtggggga ttgtggtaga ctaattttag ttagtggagg aatggggggc tttcaatgtt
     1861 gatgggtgaa ttccaacata atattgatgc aaaaggacgt ttaatcgtgc cctcaaaact
     1921 tcgtgaagaa cttggcgaaa agtttgttct gacacgcgga ttggatggct gtcttttcgg
     1981 ctatccgatg agcgaatggg aaaatctgga agctaaactg aacgaaatgc cgttggctaa
     2041 aaaagacgca cgtacttttg tgcgattctt ctattcggct gccactgaat gcgaacttga
     2101 taaacaagga cgaatcaata tacccagcac gttaagaaat tatgcagctt tgacaaaagg
     2161 ttgcgtagtc atcggtgtat ctaaccgaat tgaaatttgg gatgagacac gatggcaaga
     2221 tttttctgca gcagcagaag agaactttga cgaaattgct gaaagtatga ttgatttcgg
     2281 actataagaa taggaagagg ctgactaatg actgaaacat ttcaacacta tacagtcatg
     2341 ttgaaagaaa cggtagatgg attgaacatc aaaccggatg gcgtttatgt agactgtaca
     2401 ctcggcggtg ccggacatag tgaatattta ttaagccaac taagttctaa agggcatctg
     2461 tatgcgtttg accaagatca aaaagcaatc gatcacgcca agctgcgttt agcttcttat
     2521 gtcgaacaag gtatggtcac atttatcaaa gcgaacttca gagaattaga agaacgcttg
     2581 agtgagcatg tagataaggt agacggcatt ttgtatgatc taggggtttc ctctcctcag
     2641 ctagatgaag cagaacgtgg atttagttat catcaagatg caccgttgga tatgcgcatg
     2701 gatcagtcag ctcctttatc cgcttatcac gtaattaatg aatattccta ccatgaatta
     2761 gtaaagattt tcttccgcta tggcgaggag aaattttcca aacaaattgc tcgtgaaatc
     2821 gaacgagtac gaaaaacaaa accaatccaa acaactggtg agcttgtcga aatcattaaa
     2881 caagtcatcc cagctcccgc aagaagaaaa ggcgggcatc ctgcaaaaag aatttttcaa
     2941 gctgtgcgga ttgcagtaaa tgacgaactc ggtgccgaag aggcctcttt agaacaggca
     3001 atccggttat tgaaagtcaa tggaagaatc agcgtaatca cattccattc attggaagac
     3061 agaattgtca aaagtatgtt taaagaatat agtacggtac aagatctacc accgggactt
     3121 cctgtcatac ctgacgaatt ccaaccagaa ctgaaagtca ttactcgtaa accaatcctt
     3181 ccttcagagg aagagctaac agaaaacaat cgatcacgca gtgcaaagtt acgtattgca
     3241 gaaaaaataa gaatgaaaga ggagtagaat gatggcagaa ttaaagaaaa tgcaagatta
     3301 cgaatatgat ttacctgaat tggtggatca gccggaacaa caaatgcctg attcttcttc
     3361 gaatcaagaa atcattcttc cacaatcacc ggcaaaacga ttaaagcaga tttctgcttt
     3421 ggaaaaaatt gctgtggcaa gcgtggcgat tgctattgtt gctttatgtg ttttaacagt
     3481 tatgctgcgc acaaatatca gcggagctga aaaagaaatc acgtcaatac aaacagaaat
     3541 caatgataaa aagcaagaaa aaacgagttt agaacaagaa aaaaacgaac tttctcgtac
     3601 tgaacggatt aaacaaatcg cagaagaaaa aggattgtct attaatgacg acaatttaag
     3661 gaaagtgaag taaatgagtc ttaaaaataa aattcggcgg tttatggaaa agaaaaatct
     3721 aaatccgatg aacaatcgca aaaaagtagg tatcatttta tttgctacga gtattggatt
     3781 gttcttttta tttgccgtcc gtttctcgta tatcgttatt ggaggtcatg ttgctggtac
     3841 ctcattagct gaaaaaacaa aacagttgta ccaaggaagt gaagtagtaa aagcaaaaag
     3901 aggaacgatt tatgatcgta acggtgtggc gctcgcagaa gatgcctcct cttattcaat
     3961 taaagcgata ctctcaaaaa catatacctc tggggataaa aaactgtatg ttgaagaaaa
     4021 gaattttgat aaaatagcag aaatcctgca taagaatctt tctatagaca aaaaagatgc
     4081 actgaatatt ttagaggacg gcgcaaaaaa agagctgtat caagttgaat ttggtagcta
     4141 tgggaaaaat atctcccaag aaaccaagca aaacatcgaa gcggacatga aaaaagaagg
     4201 tgtcgctggt ctttattttg tcgaccatca ggctcggatg tatccaaatg gcgtcttttc
     4261 ttctcatttt attggatacg cagttccaga caaagatgag aatggcttag ttggtaaact
     4321 aggtttggag tctgcttaca atgatatctt gagcggtaaa gatggtaaga ttatttatca
     4381 aaaagataat tttcaaaatc ctctgccagg tacagtggca gaagaagaaa aagctgtcga
     4441 cggacaagat atctacacga ctttagacag cagactacaa agttatttag aaacactgat
     4501 ggatcaagta aatgaagaat atcagccaga agaattgaca gcagtcctga tgaaagcaaa
     4561 aaccggagaa atcttggcaa tgggacaacg acctactttc aatcctgaaa caatggaagg
     4621 attgactgga aaagatgcta tctggcgtaa tttcttggta caagatagtt atgaaccagg
     4681 atcaaccatg aaagttttca ctactgcagc tgctatcgaa gaaggagaat tcaacgagaa
     4741 tgaaaccttc caatctggaa aaatccaagt agaagatgct acgatcaatg accatgactt
     4801 tggtgaaaaa ggtgtgctga ccatgcgtca agctctttca tggtcaagta acgtcgggat
     4861 ggtcatactg gaacagcgat taggcggacg atggtataac tatttacaaa aactgggatt
     4921 tggacaaagt actcattctg gattagatga tgaagtaaac ggtgctttac ctacattaaa
     4981 tattgtcgac cgagcaatga gtgcttatgg acaagcggta ggtgtgacga atttccaaat
     5041 gatgaaagct ttcacttcta ttgcgaataa cggtacaatg attcaacctc gatatatcag
     5101 caaagtggtg gatccccaaa ctggagaaga gcgaacaact caaacagaag ttctagggca
     5161 acctttttca aaagaaacaa cagaaaaagt ccgtgaatat atgagagatg ttgtcgaaag
     5221 cgaaaactac ggtagtgctt atggtgtata tagtgtacct ggatacaatg tatcggcaaa
     5281 aaacggaaca gcccaaatcg cttctgatac agggggatat caaacaggag atacagccta
     5341 cctgtattcg attgtggaga tggttccttc tgaagatcca gactatgtct tgtatctaac
     5401 aatgaaacac cctaagacat atgatcgaat ggcattggct aaaatcgcaa atccattgat
     5461 gaaacgggca atggatttta aagaaactga agaagattca gatacagaaa caaaaacaga
     5521 aaaagtatcc gtagccgatt atcgtaatct tgaagcggac gttgcagcag ctgatgctca
     5581 aaaaagtgga ctgcaacctg tagtgatcgg taatggtaaa aaagtacaaa aacaatctac
     5641 tgccaacggt gatcagttga tctctggaga aaaactgatc ctatatactg gtggtgataa
     5701 gctgatgcct gacgtaacag gatggtcaaa agcagatatc atgaagctgg ggaaaatcct
     5761 cggtattgaa gttagttttg atggagatgg ttattgtgtc aaacaagaac ttgctccata
     5821 tgaaaaaatc actgaagaca aacttagttt tactttagaa gaataagaag agaaatagga
     5881 gagaaaagag catggaatgg acacaagcat tgattcccat tgtcagcagt tgtgcaatga
     5941 caattgccgc aatgccgcta tttattggtt attttcaaat gaaaaagcaa ggtcaggcga
     6001 ttcgtgagga gggaccaaag tggcacaata gcaaagccgg aactcctaca atgggcggct
     6061 tagtgtttct gatcggctct attttgacag gaatttgggt tggagcttgg caaaaacagc
     6121 taactcctac tcttttcatc ttgctttttg tattagcgtt atacggagta atcggctttc
     6181 ttgatgattt tataaaaata ttcaagaaaa gaaacatggg actgaactcg aagcagaaat
     6241 tgattggaca gattctaggg ggaatcatct tttatcttgt ttaccgttct gaaggttatc
     6301 caggtgtatt gaatttcttc ggactagatc tacctttagg aatcatttat ggtattttcg
     6361 ctctcttctg gcttgttgga ttttcaaatg cagtaaatct gacagatggc atcgatggat
     6421 tagttgctgg cttaggaagt atctcttttg cagcttacgg tctaattgct tggcatcagc
     6481 atcagtatga tgtattaatc atttgcttaa gtgttttagg aggtttactg ggtttcttcg
     6541 tctacaacag aaaacctgcc aagattttta tgggagatgt cggttcatta gcgttgggcg
     6601 gacttttagc ggcaatttct ataatgctaa accaagaatg gacattgctt ttgattggtt
     6661 tgatctatgt tatggaaaca gcaagtgtga tgcttcaagt tacttccttt aagcttacag
     6721 gaaaacgaat ttttaaaatg tctccaattc atcatcactt tgaaatgtgc ggatggtcgg
     6781 agtggaagat tgatacagtt ttttggttga tcagcattgt gacttcgttg atcacattat
     6841 ggatcgtttg gtaggagatg acgggaaatg aaaaaaatat caacatatga aaataagaaa
     6901 gtgcttgtct taggactcgc aaaaagcgga gtaagtgcgg ccaaattatt acatgaattg
     6961 ggcgcacttg tcaccgttaa tgatggaaaa ccttttgacg aaaatccgga agcacaagag
     7021 ctgctttctt tagggataaa agtaataaca ggtagtcatc caatcgaatt gttggatgaa
     7081 gaattttcat tgatggtcaa aaatccaggg attccctaca gtcatccttt agtagcaaaa
     7141 gcccaagaaa tgggtattcc ggtaattacg gaagttgagc tagcttacga agtagcggaa
     7201 tgcccaatta ttgggattac aggaacgaac ggaaaaacaa cgacaacaac gatgacagga
     7261 ctactactga atgcaggagc agatcaagga attgcacgtt tagcgggtaa tattgggtat
     7321 ccagcaagtg gtgtagcgca agaagcaaaa tcagaagata aaattgtgat ggaattgtct
     7381 agcttccaac tcatgggaat cacagatttc cgccctcata tagcggtgat taccaatatc
     7441 tatgaagcac atattgatta tcatggtaca agaaaagaat atgtcaaagc taaatggaac
     7501 cttcaaaaaa acatgacaga aaaagattat ctgatcctta actggaatca gtcagaacta
     7561 caagaattag cgcaaagaac aaaagcacgc gtactgcctt tctcgacaaa agaagtgttg
     7621 gaagacggtg tgtatgctga cgattactca atatactata aaaaagaaaa aataatggaa
     7681 atatctgaac ttggcgtacc aggtaagcat aatgtagaaa atgcactagc tgctatttct
     7741 gtagcaaaat tgtatggtat atcaaatgaa gctatacgag aaacgttgca ttttttccat
     7801 ggcgtaccac atcgtacgca atatgtagga gaaatccaag gcagaaaatt ttataatgat
     7861 tcaaaagcaa ccaatatttt agcgactaaa atggctctaa gtggatttga aacgtctaag
     7921 gtcgtgttat tggctggcgg attagacaga ggaaatacat ttgatgaatt aattccttca
     7981 ttaaaaggaa tcaaagcaat ggtcgtattt ggccaaacaa aagaaaagtt gatggatgct
     8041 ggaaaaaaag caggcatcga gacaatagta acagccgatt ctgtagaaca agcagttcct
     8101 ttagcattag aaaacagtac agatggtgat gtagttttat tatcaccagc gaatgccagc
     8161 tgggatcaat acccaaattt tgaaactaga ggaaacagat tcatggaagc tgtgaaccga
     8221 ttaaaataga aaaagtagaa gcgagcgata aaaaatgaaa attttggtta caggcggagg
     8281 aacaggggga catatttatc cagctcttgc ctttgtcaat tatgtaaaga ctcaagaacc
     8341 gaatgctgaa ttcatgtatg tgggagcaaa acgaggtcta gaaaacaaaa tcgtccctga
     8401 tacaggaatg cctttccata ccttggagat tcaaggattt aaacgaaaaa tttccatgca
     8461 taacatcaag acggtccgat tgtttcttaa gagtatccgt caagcaaaaa aaatcctcag
     8521 tgattttcaa ccagatatcg ttatcggtac tggaggatat gtttccggag cggtagtgta
     8581 cgctgcagcc aaaatgggta ttccaacagt tatccatgaa caaaacagtg taccaggtgt
     8641 taccaataaa tttttaagcc gatatgtgga tcggatcgct ctttcattta aagatgcggc
     8701 ttcctttttt cctgaaaaca aagcagttct tgtaggtaat cctcgagcac aagaagtagc
     8761 agatactaaa aaatcagata ttttgaaaac atttgatttg gatccagaaa agaaaacagt
     8821 cttgattttt ggcggtagtc aaggagcact taaaatcaat caagcagtat cagttttttt
     8881 aagtgaatat attccttctt cttaccaagt tttgtatgct tcaggagata gatattatga
     8941 agagatccta aataaaataa atcctgaaat acaaaatatt agtgtccagc cttatatcaa
     9001 aaatatggca gaggtcatgg cttccagtga tctgctggtg gggcgtgctg gtgcaacttc
     9061 gatcgcagaa ttcacagctc tggggttacc ggcagtgttg gttcccagcc catatgttac
     9121 gaatgatcat cagactaaaa atgcaatgag tcttgttcac gcaggagcag caaaaatgat
     9181 tgcagataat gaactaacag gtgaatcact aagtcagaca gtcaacgaaa tcatgggtga
     9241 tgaagaattg caaaagcaaa tgtgccgagc ttctaaagaa caaggaatcc ctgatgcttc
     9301 aaaacgtctt tatgacttag taaaacaaat aatcaaaaaa taaaggcggt ggtcttagtg
     9361 agtaagaaaa aagaaccaaa aaaattgtct catccgtctt ctgatgaaaa caatttgact
     9421 ccttggcaaa aggctaaccg aaaatatctg gcagaacaag agatgaaaaa ccaagagcca
     9481 aaaaaagaag acataacatt agatacaccc ctttcagatt ctgaagaaga aaaaacggtt
     9541 caagaggaac agcaagaaga aagccagtcc gagaaaaaaa tcgaattttt tgaagagatc
     9601 aagcaaaaga aaaacggtgg accatataac ggttcttttt tgaaccggct gcccaattta
     9661 aaaagtcaac ggcacaaagt actatttcgt cggttagctt tgatcatcac tgtattgggt
     9721 ataccgctga tttttttgat ctattatgtt tctccttata gcaaattaca agcagttgca
     9781 gtatcaggca ataagacagt caacagccaa gaaatcatct ctgatacaaa attgtcatta
     9841 ggagaaaatg tttggggaca gtattttcat cgatctactt atatcgatcg ccttaaaaaa
     9901 gcacagccaa gaatcgaaac agcaaatata catttcaaag ggatgaatga attcgatttg
     9961 gatgtaacag aatataaaga gattgctttg atcgcaaaaa acaatcaata ctacccagtg
    10021 attgaaaatg gaacagtatt agatgaaaaa gtagcaaatc ctacaaaaaa tctccctatc
    10081 ttggaagagt tcaaagactc tgcaaaaatc aaagaattaa caaagcaata caatcagtta
    10141 tcatcagaat tacaaaaagc aatttctgaa atcaagtaca cgccaagagc aagcaataaa
    10201 aatctgatcc aattgaatat gaatgacggc aatcaagtta ttgtaaatat caataatctt
    10261 gcgaaccaaa tgaaatatta ttcgcaagta gccaaagata tggatgaaaa aggtgtgatc
    10321 gacatggaag ttggtatctt ctcgcatcct tacggtagca ctcaagaaac ggaagaatcg
    10381 ggccaatcgg aagaaacaac tgaaagtgaa gaaaatgcgg atcaaacaaa cgaatcaagt
    10441 gaagaggcag aaacgcaaaa agcacaaaca cctggatcgt ctagcacaaa cggatcatct
    10501 gattcaaatg attcaagtga aacaagtcaa atagaagata atccaagtac aactccgtca
    10561 agtttaaatg aaaattgata aaacaacttt aaaaagtaag aatgtacttt ctaaaaacca
    10621 gtaatttatg gtagaattgg aagcagtgaa tatatctatt aataagtata ttttaaaacg
    10681 gacgctagtt gttcgcttgt atatggaata gtaagtagga gggaatccca ttcatggcaa
    10741 aaacaggaat gtatgtaggc cttgatattg gaacgacatc tgttaaagtt gtcgtggctg
    10801 aatatatcga cagccaaatg aatattattg gagtaggaaa cgcaaaatca gaagggatta
    10861 accgaggcat tatcgttgat atcgataaaa cggtccaagc gatacaacga gcagtacgac
    10921 aagcagaaga aaaagcaggt atccagatca aaggcgtaag tgtcggtttg ccagcaaaca
    10981 tgcttgaagt agaaaattgc cagggaatga ttgcagttaa cggagattca aaggaaatca
    11041 ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt tcgttcaata cctcctgaac
    11101 gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga tgggttcgag ggaatcaaag
    11161 atcctcgtgg aatgatcggc gtaagattag aaatgtacgg tctgttgttt acaggaccta
    11221 aaacgatcat ccataacata cgtaaatgcg tggaaaatgc cggattaatc gtcaacgaaa
    11281 tggtgattac tcctttagcg ctgactgaat caattctttc tgacggagaa aaagattttg
    11341 gtacgatcgt cattgatatg ggtggcggcc aaacaacaac ggctgttatg catgataagc
    11401 aattgaagtt taccaatctt gaccaagaag gtggcgaatt tgtcactaaa gatatctcta
    11461 ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa aataaactac ggggatgcat
    11521 acccagaacg gacatcacca gatgaagaat ttccagtaga tgtgattggt cagtcagaac
    11581 cagtgaaagt cgatgaacgc tacttgtcag aaattatttc agctcgaatg gaacagattt
    11641 tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt agagttgcca ggtggcgtcg
    11701 tattgacagg cggagctgct agtcttcctg gtgttgtgga cttggcacaa gaaatcttcg
    11761 gcgtaaatgt aaaattgtat gttccaaatc acatggggct gagaaacccg gtatttacaa
    11821 atgtaataag tatcgtggat tattcagcaa acttgagtga agtgtatcaa ttggcaaaaa
    11881 ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt agttgaacaa gaaacaacaa
    11941 acacatatga gagctatgaa gcacctgaag aaacaatcta cgatgagccg gaaccaaaag
    12001 agtccggaga aaatgtaaaa aataaaatca agggtttctt tacaaatatt tttgattaat
    12061 tcatcctggg aggaataaga tatggaattc tcaattgata ataatatcaa tgacggcgca
    12121 gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg ctgtaaaccg aatgattgaa
    12181 gaaaacgtta aaggtgttga attcattaca gccaacacag acgtgcaagc attaaaaaat
    12241 tcaaaagctg aaacagttat ccaattaggc cccaaatata ctcgtggttt aggtgctggt
    12301 tctcagccag aagttggaca aaaagcggca gaagaaagtg aacaatcctt acgcgaagct
    12361 ttagacggtg cagatatgat tttcatcact gcaggaatgg gtggaggaac cggtacaggt
    12421 gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg cattgactgt tggtgtcgtg
    12481 acacgtccat tcacttttga aggaccgaaa cgtggccgtt ttgctgcaga aggtatcgct
    12541 cgtttgaaag aaaatgtgga tacattatta attatctcaa ataaccgttt attagaagtt
    12601 gtagataaaa agacaccgat gcttgaagct ttccgtgaag cagataatgt tttacgacaa
    12661 ggtgtacaag gtatctctga tttgatcaca gcgccaggtt acgttaacct tgactttgct
    12721 gacgtgaaaa cagtgatgga aaaccaaggt acagcattga tggggatcgg tgtggctagt
    12781 ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct cctctccatt gcttgaaaca
    12841 tctattgatg gagctgaaca agtacttctt aacatcacag gcggtttgga tatgacattg
    12901 ttcgaagcac aagatgcttc agatatcgta gcaaatgcag caactggcga tgtaaatatc
    12961 attctaggta catctatcaa tgaagaaatg ggcgatgaaa tccgtgtgac ggtcatcgca
    13021 acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc gaccagctag acaagcacaa
    13081 atgcaatcac ctgctcaaaa gactgtatta gatatggatc aagctaaacc aacttcttca
    13141 gaagaagaaa acagttttgg cgattgggat atcagacgtg aacaaaatgt tcgtcctaga
    13201 gttgatgatt ccaattttga caatatcgaa aaaaaagaat ttgatacatt caaccgcgaa
    13261 gaaacaaaat ctcatggcga tgatgaatta aatacgccac cgtttttccg tcgtaaaaaa
    13321 taaaggagag gaagagcaca tgattgctga caacttgcag aaaatcaagc aagaaattca
    13381 ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt acattagtag ctgtaacaaa
    13441 atctgttgaa tccgctcaag ctgaagaatt ggttagagct ggcgttatga attgtgcaga
    13501 aaaccgagta gataaattat tagaaaagaa acaagattta tttgattttc ctgaaataaa
    13561 gtggcattta attggtaatt tacagcgcag aaaggtaaaa ttgattgtaa atgaaattga
    13621 ttactttcat gcgttggaca gtttaagatt agcagaagaa atccagaagc ggttagagaa
    13681 agaattatcc tgttttattg aagtaaatgt ttctggtgag gaatcaaaac atggaatttc
    13741 accagatgag cttttaccgt ttgtggaatc tttagctgcc tgcacaaaaa tcaaggttgt
    13801 tgggttgatg actatggcgc ctaaagaagc ttctgatgag caaatccata aagtgtttgg
    13861 tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt tatgcccatg caccatgtag
    13921 tgaattaagt atggggatga gtcaagactt tcaaattgcg attgaagaag gtgcaacatt
    13981 tatccgtgta ggaacagcgc tattcaaaga cgaataaagg aggaacaaaa atgccgcttt
    14041 taaacagagg agcaattgct aattttttcg gattagctga tgaggaagag tacgaatacc
    14101 aagattatcc tgaaacaaca aaaaaggctg tacagccaac taaaaaaaat acaacacaga
    14161 atcaatcaac tcattctgta tctgctgcga atactcatga aaaacaaaaa acggcgactt
    14221 cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa gaaaaaagta attgaactac
    14281 atcaatcacc tacaaaaact gtagcgaaag aaagaacagc ttcaagaacc tctacacaac
    14341 ctaaagcaaa tcttgctgga aaaatcactg taatggaacc aagagcttat tctgaagcta
    14401 tggcgattgc taaacgaatc attgctggag aagcaacctt agtgaatttt catttagtag
    14461 aagaaaaaca agcgagaaga atcgttgatt ttctaacagg aacagtttat gcactagatg
    14521 gagatatcaa acgtgttgga gatgagattt ttctttgtac accgactggg atggagattg
    14581 acagttcaac cgctcaatct tttgcagatg cgaatttgtt tgatctgtaa gaggaggaac
    14641 tacgatttat actattgcac tattacttca tttacttcaa cgggcagcct atttctatag
    14701 cattatcttg atggtatatg cattgctctc ttggtttcca ggcgggtatc aatcagggct
    14761 aggacgatta ttagctaaga tctgcgaacc gtatatcagt ttgttcgatc gtctgccttt
    14821 gaggattgga cccttggatt tcacaatttt aggggcaatc ctgatcttga acttagccgt
    14881 acaaggttta gcacagatcg ttatatggat aactgtccat ttagtttatt gattgaagca
    14941 acaaaatctt tattctcaga atagatagga ggggaaaatt ttggatgcaa atgtatacca
    15001 gcactttcgg gcagatgaac gtacatttat cgattcggta ggcgattgga ttgaacaggt
    15061 tcaaggacaa tatgcaccct atctgaccga atttcttgac ccaagacaat cctatatttt
    15121 agaaacactg attcgacaaa gcagtgattt gcgtttcatg ttttatggcg gatatgaaca
    15181 ggcagaaaga aaaagatgtt tgattttccc cgattattac gaacctgtag aggaagattt
    15241 tgaaacagca ttgtatgaaa tccattatcc tagcaaattt gccagcctaa gtcatgggaa
    15301 aatccttgga actttagtgg gaacggggat caaacgctct tactttggcg atatcatctc
    15361 ggacggtgat cgctggcaag tatttattgc aaatgaggtg gatcattttg ttgctgcaca
    15421 agtaacaaag attggaaagg ttgctgtacg cttggaacca atcaaataca cagaaatgat
    15481 tatgccgaaa gatagctgga cacaagaaag aggaacagtt agttccatgc gactagacag
    15541 tgtgatctct gagatttata atatttccag acagcggtcc aagcagctga tcgaagctgg
    15601 taaagtaaaa gtgaactgga cagaaaatac aagacctgat tttgtacttg aactattaga
    15661 tattatttca gtcagaggtt atggcagagt ccagatccaa gaacttgagg ggaagacgaa
    15721 aaaagaaaag tacagggtac tgttcggtgt attacgtaaa taaaataaaa aggtggttta
    15781 ctcatggctt taactccatt agatatccaa aataagacat tcccgacaaa aatgcgggga
    15841 tataatcaag acgaagtaga tgatttctta gatttagttg ttcgtgacta tgaggaattg
    15901 actcaaagaa atcgagaatt agaaaaagca gtcaaacatt ctgaagaaaa acttgagtac
    15961 ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg tagctcaaga tacggctgat
    16021 aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg ttacttctgc acaaaacaaa
    16081 gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc aattaacaac tgatgcagaa
    16141 gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag caagacagct tgcgacagaa
    16201 actgaagatc ttaagaaaaa aacacgagta ttccatcaac gtatcagctt gatgcttgag
    16261 tctcaattag aacaagtgaa aagtccggaa tgggatgaaa ttttacaacc attctccagc
    16321 tacgtgactg acagccatga agtgatcaaa gaagttttag caaaacagct tgacaatgaa
    16381 aatgatactg atgtaaactc agacacagga gtcatagaag cacctatgac ggcatttgat
    16441 acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg aaaacgagta gaacagaaaa
    16501 gcactttgtt attcttaaag cgagccgatg agagtggaag ttcggtaaga cactgcatgg
    16561 tgaaagatcc tctacttgag acctgctttt ccgaaattag agtaagaagc agcggctgac
    16621 ctcgttattg gtcacagaga gtttgcacta gatagatgca actaaatttg ggtggtacca
    16681 cggcatgttc gtcccttgcg gctgaacatg cttttttgtg tccggaaaac aaataaagga
    16741 gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag gaaaaacaaa attccctatg
    16801 cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag aatgggaaga aaatcgaatc
    16861 taccaaaaaa gacaagagtt gaacgaagga aaacctagct ttgttcttca tgatggacct
    16921 ccatatgcca atggtaatat ccatttagga cattcattga ataaaatcag taaagacatt
    16981 atcattcgtt caaaatcaat gtctggctat cgtgcaccat atgttccagg atgggataca
    17041 catggattgc cgattgaaca agtattggct aataaaggcg tgaaacgtaa agaaatgtcc
    17101 atggcagaat acttggaaaa atgtcgtgaa tatgcacttt cccaagtaga taagcaacga
    17161 gcagatttca aacgcctagg agttgcagga gattgggaac atccttatgt tacattagat
    17221 ccttcttatg aagctgcaca agtacgcgta tttggtaaaa tggctgaaaa aggctatatt
    17281 tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg agtcttcttt agcggaagct
    17341 gaaattgaat ataaagatgt aaaatctcca tctatttttg tagctttcaa tgtcaaagac
    17401 ggcaaagata ttcttgatga agatacagct tttgtcattt ggacgacaac accatggaca
    17461 ttgccagcaa accaaggtat tgctgtaaat ccaaaattta cgtatgtatt ggtggaagcc
    17521 gatggccgta agtttgtcgt agcgaaagat ttgctagaaa cagtccaatc agaaatcggt
    17581 tgggaaaatg taacagttct aaaagaattt gctggacaag aaatggaata tatgactgca
    17641 agtcatccat tctatgaacg tgaatcactt gtgattttag gcgatcatgt tactttagac
    17701 gctggtactg gattagtcca tactgctcca ggacatgggg aagatgacta tatcgctggt
    17761 agcaaatata aactatctgt agtgtcacct gtagactcaa aaggattgtt tacagaagaa
    17821 gcacctggat ttgaaggtat cttttatgac aaagcaaatc ctatgatcac tgatctatta
    17881 aaagaaaaag gagctttgct gaagctagat ttctttactc atagttatcc tcacgattgg
    17941 cggacgaaaa agcctgtgat ctatcgggca actccacaat ggtttgcatc tatcgataaa
    18001 ttccgtcaga atattttgga tgaagtagaa aaagtagagt gggttatccc atggggtaaa
    18061 acacgtttgt ataatatgat ccgtgaccgc ggcgattggg taatttcccg tcagcgtgca
    18121 tggggagtac cacttccgat tttctatgct gaaaatggag aagcaatcat cacaccagaa
    18181 acaatcgaac atgtggcaaa attatttgct gaacatggat caattatctg gtttgaaaaa
    18241 gaagcgaaag aacttcttcc tgaaggattt acccacccag gttctcctaa tggcgaattt
    18301 acaaaagaaa aagatatcat ggatgtctgg tttgattctg gttcttctca tgaagctgtt
    18361 ttacgtcagc gaccagaatt atcattccca gcagatatgt atttagaagg atcagaccaa
    18421 tatcgtggct ggttcaactc aagtatcacg actagtgtgg ctatcaatgg tgtagcacca
    18481 tacaaatcag ttatttctca aggatttgct ttagatggtg aaggccgtaa gatgagtaaa
    18541 tctttaggaa acatcattgc accagaaaaa gtgatcaaac aatttggtgc agatatcctt
    18601 cgcttatggg tatctagtgt agattatgaa gcagatgttc gtgtttctat ggatatctta
    18661 agtcaagtgg cagaagtata tcgtaaaatc agaaatacga tgcgtttctt attagcgaat
    18721 acagaagatt tcgatcctaa aaaagatact gtttcgtaca atgatttacg ttctgtagac
    18781 aaatatatga ccgttcgatt gaaccaagta atcaaagaaa tccgagaaga agggtacgac
    18841 aaatataact tcatgcatat ttaccgtaca gttatgaact tcttgacggt tgatctatcg
    18901 tcattctacc tggattttgc taaagatgta gtttatattg aagcagaaga tgattatcaa
    18961 cgtcgttgca tgcaaactgt tttctatcaa acagctgtag ctttgacaaa attattgact
    19021 cctatcattc cgcatacagc agaagaaatc tggtcattcc tgaaagaaga agaggagtac
    19081 gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa accaagaaga attgatggat
    19141 acctggactg ctttcatgga cttccgtgat aatgtcttaa aagcattgga agaagcaaga
    19201 aacagcaaat tgattggtaa atctttggaa gcaaaactaa ctgtttatcc aaaacaacaa
    19261 gttcgtgaaa tgttaaaagc tttggatgca gatattgcac aattattgat cgtttcacca
    19321 gactactttg aagttatggc agcagatgaa caagtgccag ataacgctat ggtatttgat
    19381 gatgttgcta ttacagtgga aaaagcagac ggggaaactt gcgatcgctg tcgccaagtt
    19441 cgtaaagatg taggagtaga tgaaaaactt cctcatctat gcggtcgttg tgcaaaaatc
    19501 gttgaagact tctatcctga agctgtagct gaaggattcg aagaaaagta aaagggaaaa
    19561 gggggtgtga catgttcttt gtcacacccc cttttgttat caatcgttta attttccacg
    19621 ttcacgatac cagttatctg gttgccagat ccattcgaat ccgtcttttt caagtagttc
    19681 gaaagctgct ttaggtccca ttgatccagc tttgtagttg ggaaaatccg gttgtgtttg
    19741 gtcccaagct tttcgaatga catccacgat atgccaagat tgagcgactt cttcccaatg
    19801 agagaagttc gttccatcac cattcaacgc atctaatagt aatttttcgt aagcttctgg
    19861 gctattttca accatttctg cactatttct aaattccaat ttgattggtt ccgtttcaaa
    19921 tccttgccct acttctttcc cattcaagga taaagaaaat ccttcagttg gctgaatata
    19981 gatcgttaga acattaggtg gaagtgtcgt atcgtcacat ggttcatcga cagatgtttt
    20041 gaatacatta acaggaactt gtttgaatac gatgttgatc cgagtccctt tttcagtcaa
    20101 acgtttgcct gtacgtacat agaaaggaac acctgaccaa cggaagttat cgatagtgaa
    20161 tttgcctgcg acgaatgttt cagtctctga ttgagcatcc actttgtctt cttcgcgata
    20221 gcccttgaat tgtacaccat ccaattcacc ttgagcatat tgcccgcgaa cgaagttttc
    20281 taacacttct tcttcgttat aaatgcggat agctttcaat gctttgatct tttctgtacg
    20341 gatttctttt tcggaaaatg ctactggcgg ttccattgca agtaatgcta caacctgtaa
    20401 aatatgattt tgcaccatgt ctttaagtgc gccactatga tcgtagtagc ccccgcgatc
    20461 ttctacaccg aggttttcgc caaaagtaat ttggatattg tcgatatagc gattgttcca
    20521 ttgagcctca aaaatattat tggcaaaacg gatagctgag atgttttgga tcatttcttt
    20581 tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg aatacttctc tgatttgctg
    20641 gttcaattca aaagcagact ggtaatcaga accgaacggt ttttctatga tcaaacgatt
    20701 gaagccattt tttgtcataa tattttgtga tttcaaatga gaaacgatcg ttccaaaaaa
    20761 ttgaggtgcc atagccaaat aatagacacg gttaccgttt aattgatagt gtccatctaa
    20821 tcggtcagac aattctttta atgtctgata atgcgttgaa tcatttacat catgggattg
    20881 atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct gaaggattca atgcacggat
    20941 tgtttctttg acaacttcac ggtagtgctc atcgctccag ggacggcgag cggttccgat
    21001 tacagcaaaa tgctcgccta aatttccttt tctgtaaagg cggaacaaag acgggtacaa
    21061 tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta aacaatacat ttttttcatt
    21121 agacatgtgg atacactcct ttatatttta tcgacaaaaa tcgtgctggc cgccttaaag
    21181 ctgacgggga ttttctttct atcattttgg atagtgatcg gtccttcata agctgcgatg
    21241 tcaacgatct catattcttc gttgatattc aggtcaatag tgactaagta atccaacaat
    21301 tctttttcat caagcactcg tgcgatacgc acttttgtac cgacaggata ggactccaat
    21361 gtttgtcggc gttcttcatg gacaacttcc tcttttttag gaatcgatcc tccatgagga
    21421 caatgttcag gatgattcaa atagttttct aaatgatctg caagatgttc cgaagtcaca
    21481 tgttccaata cttcagcatc atcatggact tcattccagc tatagtctaa atgttcaact
    21541 aaaaaaactt cccataagcg atgtttgcga atcagactgc tagctttttg cagtccgctt
    21601 gcagtcaatt gtacaccttg ataaggggaa tgttctacaa gctcttcttt cacaagttta
    21661 gaaatcattt cactgacaga tgcagctgag acatctagac tagatacgat ttgtttattg
    21721 ttgactttcg ttgtatctcc acctagttct aaaatgattt tcaaataatc ttctcgattt
    21781 ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc attagtaagt gtaacaaaaa
    21841 aaacgttaat tgactatttt tctaaataaa aaacaaagga tttttagacg tttttacaac
    21901 aaaaaacgaa agatgataga gaagactcat tctttcgttt ataaaggata tatatataca
    21961 ttgggaggga tctatttttc agataacaac ttatatgaag tgtctgcaga ttcgtcataa
    22021 agagtttgtt cgggataaga aaagataaga taaaaaccat actgatcacg tacgactaaa
    22081 cgatattcat ctatttctac cacagatgtg ccaagagact gaccgttgat caaaaacgtg
    22141 taatcagatg taatctgtaa ttgttgtttt tgctggctat cactaaagaa ccatgtacct
    22201 tgatactttg agagtccttc tgacttttcc gcttgttttt tctttttcaa gtaaataaaa
    22261 cttgagagaa cggtaatgag tgaaccaaga aaatagatca ttttttttat caccaaatca
    22321 ctcctttatg agaaaataat cgcatagaca tattctttct tctttatttc attgtaccga
    22381 ataaaagcta aaatttctct aagactttcg actggttttt ttactgtttt tctacaaatt
    22441 atgataggat acatagtagg aagaaaggtg acataatttg aagtatcagc aagtggaaat
    22501 agcttttttt attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa aagacggaga
    22561 aataattatt gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga taccaggcgc
    22621 tgaagtggct attgtatttg ctcctggacc taaacggaaa accgcctatg atctgattgc
    22681 tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat
    22741 tgatggtatt ttagacggca cgatcctttt accaggatta actggtccat ttacggtata
    22801 caagcgtgaa gtaacttatc ttcactccaa atttgatatt tatttagaga cggtggtcgg
    22861 acaaaaagca tttgttgaag tcaaaggtat gacacttgaa aataaagcga ttggtgcttt
    22921 tcctgatgcg cccacactca gaggattgaa acatatagga gaattaacgt atgccgcaca
    22981 agatggttat gccgcttatg tcttgtttat tgctcagttc gaacatcttc atctggcaac
    23041 gatccatgaa gaaatgcaac cagcactagc tgatatggtt cgacatgcgc agcaaagcgg
    23101 agttcaaatc ctcgcctata attgtcaagt atcggcagat gaggtaactg tcaagcaggc
    23161 cattccattt gatgtagata tcccttttga agatcctata caatagaaag aaggtaaaat
    23221 aatgatcaac ttaggtatta tcggaacaaa ttggattact catcaatttg tccaagcagc
    23281 tttaagcaca aaaaaatatg aattgacagc ggtatattct agacatttgg aaaaagcaca
    23341 ggaatttggt tctcattatg aaggggatat cgaattcgca acagatttga agactttttt
    23401 caatattact catatggaca ccatttatat tgcttctccg aatagtcttc actttagtca
    23461 agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac
    23521 gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt atttttttga
    23581 agcagcaaga aatatccatg aatcaggttt taaaaagatt gccgattact taccattaaa
    23641 agacgagata atcggtgcaa atttcagcta tatgaaatat tcttcgcgct atgaccaagt
    23701 attagatgga gaagaaccga atatcttctc gactcatttc tcaggtggtg cattgatgga
    23761 tttaggtgtt taccttgtat atgcagctgt tgcttggttt ggaatgccaa aagaagtcca
    23821 ttatttccca agaaagattt ctactggtgt agatggtctt ggatggggga tcttacgtta
    23881 tgagaatttt gatgtatcga tccagccagg taaaatcggt gattcttatt taccgtcaga
    23941 gatttatttt gactcaggaa cgctagtgat gaatggagtc aatgcaattg aacaagcaca
    24001 atttcatgat cgcgcgcata aagaaatcat tgatttggcg gtaacagctg cagaaaatcc
    24061 aatggcagat gaagcagagg attttgccaa tgtcatggct catccgaaag atccagcttg
    24121 gggattgttg tatgaagagt ggatcgagct agctagaaat gtgaaccaag tgatttatga
    24181 tttaagaaaa aatggcggca tcaaattcga tgcagataat gaggaagatt tttaatgaac
    24241 gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat atgaggataa agggtttatc
    24301 tcaccttctc ttattcaacg agcagttttt cagccactaa gtaataaaca aagtatagta
    24361 gcggtttctc ctacaggttc aggtaaaaca ctggcttatc tttggcctct actactaaac
    24421 gtggaaccag gtgaagcaag tgcgttggtc atttttgctt cttctcaaga actagctatt
    24481 caagtagcag atgttgcacg tgaatgggga aaagacaaag agttgaaagt ccaatctttg
    24541 gtgggcggag ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc agaaatcttg
    24601 attggtacac caggaagaat attggaattg atgaaagcaa aaaaaataaa agcacatcaa
    24661 gtgaaaacaa tggtatttga tgaagcagac cagctatttg acgctgggaa cagccaaatc
    24721 attgatcaaa ttcttcatca agctccaaca gagtatcagc tagctttttt ttcagcgaca
    24781 gcagatcgtt cccttgaaag cattgaaaaa atcactggga aaacgtttga gacaattgac
    24841 gtgacagcag aggacgattc tcgaaaagga ctgagacatt actttttacg tgtaccgata
    24901 cgaaagaaag atgactactt gagacgtctc acgcatatag aagattttca tggtctgatt
    24961 ttctttaatc aactgactga attaggcgta atggaagaaa aattgctgta tagaggtgta
    25021 ccggtaggaa gtctggcctc tgatcagaac aagctactcc gaaaagcagc gctagaacaa
    25081 ttcaaaaaga aaatggtcag agcacttttg accacagatg tggcggctag aggtttagat
    25141 attacaggtc ttccttatgt agtgaatgca gaagttccgt taagtgaaga agcttatttg
    25201 catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa tagtaattac actcgtacaa
    25261 gataataatc taagagattt aaaacggctt ttacgtccat caaatataac acttgaagag
    25321 atttttttat acggaggtaa gctgcaaact gagcagccaa ccaaagaaga tgcagctcct
    25381 gcagtaaata aaaagtatgc gaaaactttt tctggaaagg agcagaaccc agaatataac
    25441 aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa taaaggagca
    25501 aggcgcaaat aagcgccttt ttatgctatg attatgaaaa cgggaacaaa attggttgcc
    25561 agaggagggg ttatgtgaaa gaacgtcaag aagagatctt gcagctattg acagaaaacc
    25621 aacaaggatt gacagcaagt gatgtagctg agcgcttaac tatcgatcga agcaatgcaa
    25681 gccgatattt aagtgaacta tacaaggccc atcatattgt aaaaaccgct ggccgaccag
    25741 tcgtttactc tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc gactcttcta
    25801 cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc agcatacagc
    25861 aggcaaaagc tgcgattctt tatccaccgc gggggttgca tacgattatt tttggagaaa
    25921 ctggtacagg gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc gattcagaga
    25981 tgctttctgc agatgcgcca tttgtttctt tcaattgtgc ggattatgct caaaatccgc
    26041 aactgctctt tggacacatc tttgggatca aaaaaggggc ttacacagga gcagctcaag
    26101 atagtcccgg tctaatagcc aaagcagacg gcggtatatt gtttttagat gagatccacc
    26161 gtttaccgcc ggaagggcag gaaatgctgt ttactttcat tgataaaggt atttaccgtc
    26221 cgcttggaga aagtgcacaa gtacatgaag catctgttca gatcattggg gcgacgactg
    26281 aatcttcaga aagtttctta tctacgttca atcgacggat tcctatggca atcaccttgc
    26341 cgaatcttgc ttcacgttca ctggatgagc gttatgaaat catctctctt tttatcaaac
    26401 aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt cttgccttca
    26461 tgctttatga tgcggaagga aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg
    26521 caaaatcttt tttacattac cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag
    26581 aactttccct tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg
    26641 accgattcat ggacagcaaa agtcagtatt tgacatttga accaggattt gctgatgttg
    26701 tctggtcaca agatccagaa cgaaacatgc aggtctataa cgatattgag gaaaaagtca
    26761 cttctctttc cgaaaccggt gtggagaaca ttgatctaga gaccctcatt tcaaaagatg
    26821 tggatgctta tttccagaca tatgtcaaag aattgactaa aagttcgatt cccaaagatc
    26881 ttttgccaga tgaaatctgg cggttaacca atcaattgta tgatgtggct gaaaaacggt
    26941 tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg caaagtacat
    27001 tggatcgtgt gagagaaggt catatgatcg tccacccaga tttgaataac gtacggaaaa
    27061 attttaagag ggaattccag gtagcaatcg atctttcaac gatcattgaa gaagaagaac
    27121 aagttgagat tccttttgat gaaatcggct ttattagtat gtttctttct atcaatctag
    27181 gagaaagtga gcctgttaaa gaaaacaaag tagatgttct catattgatg cacggcagac
    27241 agactgcttc aagcatgttg gagatggcac aagaactatt aggaacaaaa gtagggaaag
    27301 cttttaatat gccactggtt atggaagttc aaacaatgta tgaagaagtg ttacaatatg
    27361 tcaaaaacta tcaagagcat ttgacgaatg gcctactctt gttaacagat atgggatcat
    27421 taaacacctt cagtgatttg atttatgaag agactggcat tcggacgaaa gctatcacga
    27481 tgacaagtac catgattgtg atagaagcga tacggatggc aagtgttgga agaagcttag
    27541 aagatattta tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat cagttcaaag
    27601 cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg
    27661 aaggagtagc tgccaaatta tatcagcgta ttatgccagt gattaatcaa aataaggtag
    27721 aactgatcca gatgcagttt ttagaacgtg aatcattcaa aaaacacatc gatgccttat
    27781 tggaagaata tgagatcaaa gcaattgcgg gaacagttga tgttgattat caaaatatcc
    27841 catttttttc agcctatgac gtttttgatg atgaaaaact aaatatttta aagcgtatag
    27901 ctgatgatga gatcccgaca gctaaaattg tcgattcact tgctggatca ttaagcgatg
    27961 tcttttctat tgatgaactg atccgttcat tgcaaaagac agttcagcag atccagaccg
    28021 atttgtcgat tatcgtggaa cctggagtag atactggaat cgttatccat ttagccttca
    28081 tgttagacgg attgatcaaa caagaaagcc atcggcaatt tgagaaactg tctattttct
    28141 caaaaattta tcgtttggaa atggatatcg ttcgggcaaa cttgatggca ctagaacgaa
    28201 aatatcaggt aaaaattcca gaagatgata tcgcatttct tacacaaatg tttcttgaaa
    28261 ataaaataaa aaaatgaaca cactaatcat actgtttaca cactaaacat ctgtgtgcgc
    28321 actataaaaa agagctaaaa agcatgaaaa cgctatttca gctctttttt ttattggcat
    28381 gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa
    28441 acaatcatgt tagtttgttc agcagggatg agtacgagtt tattggtcac aaaaatgcaa
    28501 aaagcagcag aagaaaaagg gatggaagcc gatatttttg cggtctctgc ctcagatgca
    28561 gataataatt tagaatctaa gaatgtcgat gtattattat taggtcctca agtacgtttc
    28621 atgaaagctc aatttgaaca aaaacttgcg cctaaaggta ttccattaga tgtcatcaat
    28681 atgcaagact atggcatgat gaacggagaa aaagttcttg cacaagcaga aaatctaatg
    28741 aaataaatca agtatgcagt tgggggtata acaacgtgga agatcaacaa aatctagaag
    28801 ccatcatggg gttaatcatg tttggcggta atgcaaagag cgatgcaatg gaagcgattg
    28861 cggctgctaa aatcggtgat tttgagttag cggataggaa aatcgctgat gcagaagaat
    28921 cacttataca agcgcatcat gcacaaacag acatgttgac acaagaagca caaggaaatc
    28981 atatgcaagt gacattattg actgttcaca gtcaagatca cttaatgaca tctattgcgt
    29041 ttactgattt agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc gaataaatac
    29101 aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg attttttatt ttgttataag
    29161 gagaactctc tttatataaa aaacttatag gaggaagtaa aaaatgaacg gactatcaag
    29221 ttggatggag aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt
    29281 tgctttgcgt gatgctttta tcggcacact gcccgcaaca atggcgggat cagtcgctgt
    29341 aatgatcaat gcaattattc gtgatttacc gcaacagttt aacagtacct atgctgacag
    29401 tttagcagca gacaaaggaa tctttgccgt attgaatgtc atcatcggaa tcaatggttt
    29461 tgtctggaac ggtacactag caatcgccgg attgatcttt gccttttctt gggggtataa
    29521 tcttgcccga gcttatggag taaatgatct agctgggggg atcgttggtt tagctacctt
    29581 gattcaaggt atttcctttg gtccagcatt atcagcagca ttagatgtga atgttccttc
    29641 aaatgtcgca gatgcaatca atagttctga gattggtgca acgattgcag atggcacact
    29701 gtcagttgga ttatggggtt ggctaaaact agatcatttg aatggaaacg cttattttac
    29761 agttatgatc atgggagcta tttcagtcat tattttttgt aaattgatgc ttgccaacat
    29821 tacgatcaaa atgcctgatt cagtaccacc agcagtatct aaagcatttg cagccatttt
    29881 accagcgacg atcgctttat acgtcattgc tattatcaat tatatcgtag gtcgtgtagc
    29941 tgatgggcaa ctgatcattg atttggtgca aaaatatatt gcagaaccat tcctaggatt
    30001 atctcaaggg ttgggagcag tattgatcgt cacagtattt gttcaagtat tctggttctt
    30061 tggtattcat ggaccaaatg tattggcacc agtgctagag ggaatctggg gacaagcaca
    30121 actaatcaat attgatattt tccaaaaagg ttataacgga aaaactggta caccagccgt
    30181 gttagatgca atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt
    30241 tgcatggttt ggcggatcag gcggaacgat cgtcttactg atagctatct tgattttctc
    30301 taaacgtgca gattatctaa cagtagctaa attatccatt gggcctggta tcttcaatat
    30361 caacgaacca gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt ttatcccatt
    30421 cttgattgct ccagtagtag caacagcaat tggatggaca gctacttatc ttggcctagt
    30481 cgctccggta tcgcagcaag tcacatgggt agtaccgccg ttcttattgt cattcttagc
    30541 aacaggagcg gattggcggg caccgatcgt aacattagta tgtatggtcg tgactttctt
    30601 gatttgggca ccatttgtca tctctgcaaa caaaatggac cctgccgcag gcgaacaaca
    30661 ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt cctcataatg aattttcgag
    30721 gaggaactga aatgattaca attgccagtg caatgcaaca agatgaaatc aaagaattgt
    30781 tagaaggata caatgaagaa ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc
    30841 tgttctttga aacaagtgca gaagatccgg aagttgcggc taaaaaagca aaggaactaa
    30901 tcaaagcaca gccttgggga acagtccttt attttcaatc aacagctgtt tagaaaatgg
    30961 tttaaaaaga taggatggtt gagatgataa aaaacggttt atttttatgt agtattggtt
    31021 ctttgatcgt gttgtatagt ttgaatccac attcgcaaag gtatgatttg tatagtatgg
    31081 tcacaggtct ttttctaaca ggattaggaa tctatttctt catgaagggg aaaaaaagag
    31141 aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa aaattaaata ttccagcatc
    31201 gtttttttac gataaagtta tggactctgt ccttttcgat gtacgtaaag cgaccggcaa
    31261 atcagtaact agaaaacagc tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa
    31321 ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag tcttataagt ttcgtacatc
    31381 gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa ccattggacg attcgacctg
    31441 tgaggttcat tacacagaaa aaatggagtc ttatggcatg cttcaaaagt tgaatgatat
    31501 gcttttagga acaatcttga tgtattttaa acggagacgt ttcaaaaaaa tgctagatat
    31561 gatcgaagca acgtattaaa tcaatgaaac atggtactat ttagttattg aaaaatgaag
    31621 taagctagaa ggaatttttc ataggaagat agatctttcc tgcgaatcct cactagcttt
    31681 tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag agctaggtgt atcttcgatt
    31741 gaggaaatgt ttgatttagc cacttatgca tttaatgggg cagatacacc tgaaaggcga
    31801 gaacgttttc gtacacttgc ggagaattct tggaattacg gattttttga tgaggaggat
    31861 cggctaacaa gtcaagtcat ggcgacccct tttccagtga atttttacgg acaagagtac
    31921 ctaatggccg gcattggcta tgttgcttct tatccagaag caaggggaca aggcgggatc
    31981 aatcagatca tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag cttatcttat
    32041 ctggcgccat tctcctatcc cttctatcgg cgctatggct atgaacaatc atttgataaa
    32101 atcagttatc aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata
    32161 aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa gaatttatga acagatgccg
    32221 gaaaaccaac gagctggtct gaagcgcgaa gactggtggt atacgtataa attcaagcaa
    32281 aaaggcaatt accagtttgc tttgcatttt gatgataatg aggaagcaac gggatatatc
    32341 gtttatcagc ttgcaacgtc ttcctttatt attgcggagt ggggattttt ggatcatgat
    32401 gctttttgca gccttgttcg cttcgtaagt tctcacaatg gtgcttttga aacatttgaa
    32461 tattcatgcg gctacggcgg taatgatcaa aattatctat tagaaaatcc atttgcttct
    32521 gtaatgatca caccttatat gatggcgagg atcgtagata tttctttgtt tttggaaaat
    32581 tatccattta aaaagagaca agctgcattt gccctacaaa tagaagaaga tcattatgcg
    32641 aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa
    32701 gtagaacaaa caaaactccc aatcgttcga ggaagtatcc aacatatcac gcagctatta
    32761 ttagggtatc gtaaagtgga agagttggta ttccaagaaa gaatacaggt gacaaaagaa
    32821 tatgaagaaa cgttaagctc aatcttgcca cagcaacagc caattcttaa tgactatttt
    32881 tgataaaaaa cgggtgatga caatattttt gtcaccaccc ttttgttttt catttggcat
    32941 ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt
    33001 ttgttttagg gttttctttt ttgtggtctt ggattgcttc tttcgtaatc ttttcccgat
    33061 tttttttaga tgaatctaag cgttctgcgt atactttttc ttttggctta tcattcattg
    33121 gaaaggacct ccctacatta tttcattcta cctctagttt atcatctgtt aaggactagt
    33181 ggaaacgttt tgcctaactt ataagctgat ttttttagca gataagcttg caagtaaaaa
    33241 acaaacaagc tacaattgat ttattgaaat tgttcctgta gctcagtagg atagagcaat
    33301 cgcctcctaa gcgataggtc gctggttcaa ttccagtcag gaacgtataa ataacccctg
    33361 tatcctttgc ggtacagggg tttgttttgt atttgtgcca aaaatgtgcc aaactagtta
    33421 tcatttttat ataaactatc gaacacagta ttgatttttt ctttatcctg atcttcaagc
    33481 tctcgaacaa tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt
    33541 ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg tgtatgcctt
    33601 agtccatgca aagtaaattg cttttcaatc cctaattctt ttaatttttt gcgcaaatat
    33661 ttgctgacgg cgtttgatga aactaatcca ttttttaagt tgaaaaaaac aaaattatga
    33721 gggtttttga tttcaaaatt ctcgaataat tctttttgat tgattttaaa cttttttaag
    33781 agatcaatcg tatgtttatc aatggaaatt ttcctatttg aagtttcgtt ttttgttttt
    33841 ccccattcat ttaatttata attccatgtt ttattaatag tgatgatttg atcttcgaaa
    33901 tcgatatctt cccatgttaa tcctagaagt tcagcaaatc gcattccagt ggcaccagca
    33961 actaacacga gcataggaga agagtactgc gcagaaaggt tttcttctac gagtttcatt
    34021 aaagatttaa attgatcata atctaaatat ttatcttctt ttggcttcaa tgaatctttt
    34081 cctttgatta ctgcttttct agttggatca aacggaatta atccttcttc tacggcatct
    34141 ttcaacgacg ctctaatatg gttattaaac ttaataacgg tagattttac atggtcttta
    34201 gcatacttat ttaaaaagcg ttgatatccc actctatcta aatcagaaat caataccgct
    34261 ggcatatatt tctttatgtt cattaacgta tcttcgtatt ttctataagt gataggagat
    34321 actgttcctt ctttataaag ttgcatccaa tcctcaaagt aatctgataa taatagattt
    34381 ttgcgatccc ctttaaggcc tttgttcaag ttatattcta attcgttggc ggcatctttt
    34441 gcttctcctt ttgtcttaaa tcctgatttt cttatcttcg tgtattttcc gtcatctttt
    34501 ttgtatgaaa tttcgtattg ccaactattt ccacgtttga ctaatcttgc cataattgaa
    34561 tcagctctct ttctctgata caataggaac tataaagaag cccattgttt gggttttatt
    34621 attttttgca catccttttt gcttgccggc gcaaggatgt gctatttgtc gttttaatgg
    34681 tataattgga tgtgtattag atcatccaat cagaggtgtt tattatgatt aaagtgattt
    34741 ttaaaagaat aactgaactt ccagagttta gattatttgt tgattattgg tatgtctggt
    34801 tttttattgt tgcattgctg ataggtctag atgtcatatt agaaagaaaa cttaagtaag
    34861 agttgagtta ataaattgaa ttcctaaatg agatattttg tagcccatat tttgtttgcc
    34921 gacatcacct aatactatgg agctaatcgt tttttctaac agaccaaaat cagataatct
    34981 atttaagcta ccatctatta gctcttgtga atagccagac atgctgatat ttccagtgaa
    35041 attttcgttt ccacgactca ttaagcatgc taacaattga atctcaatcg atgttaattg
    35101 attcagtaca agaagataat actcctcgtt atcccatgaa gcgttgttaa ttcgaagtaa
    35161 acaattctta taaagatttt tatacatatc tattttattt tgtgcctttg atttttctac
    35221 ctcgtcattt atcccttcga atattccaat gagttggtcc ttgttctcta agttgaaaac
    35281 atcttctgga atttgatcgc gaatgttttc taatgattcg tttaaatttt tgtagaattt
    35341 ctcgatgcgt ttgaaacgct tctcgttttt agacccaaaa taaagtgact gcacaacgcc
    35401 accaatataa ggaattgctc caataccgcc ttcaactatt aattcaagat attctttttt
    35461 ggttagtttt tcattgctat tatccatagt tacctcctat tttgtcattt cctttacaag
    35521 cattgtgact cctgatggca ctcttttgta taggttttat tattgtgcac aatctaactc
    35581 ttagtggggc gtggttcatt tattttttac ttactttact attaatcata tcccattgag
    35641 cataggattt ttctatgttc gatcctcttg aactaatttc tcctgtaaaa gttactttat
    35701 cgccctttgt aaaggtatca gcattgttta agtccttcgc aaaaattaca tatgaaactt
    35761 taggtgttgc agaaatatca ttccaacttt tatcatcgta ctctttgtct gctataatgg
    35821 ctattctagt agtcattgat tcgataacag ttccttcaaa agtatatgtt ttaccactaa
    35881 tatatttagt aaaagctgta ttttgctcag tagctggagt aatagaataa taactatcta
    35941 agaaagcacc ccagtcatga ttatttgtct ctaaaaattc ttcaaattga gttaggcttt
    36001 tattgctgct gttagatgta gtagtttgag tatcagatga tgagctttcc tgtttgttat
    36061 tgtcatttgc ctcgtttgat gaggaaagcg agaaacctac ggtagtaaaa ataatcccga
    36121 taataaggag aatcataggt gttttcttat ttgttttttt gataaatctt acaattaaca
    36181 tgattaaacc tataataaat aaaacaatac cagtaaaacc aaatataccg tccattaata
    36241 gatcctcttt ctcttttgat attgaatatt ataaaccaag caattctttt ttcttaaaat
    36301 cgaattcttc ttgattaata atcccttcat ctaacaactc tttatacttt ttaatttcat
    36361 ctgcacttga aatttgtgtt tctttggaat tattatctaa tatagattgt tgcttctcta
    36421 tatatttttt taaattttcc atttcattcc aatatttttt tgtaaacata acggtgtttt
    36481 catctttcgt ggccgctaat atacctccgc gactttcgtt tccgcctagc aaggtaaatt
    36541 gtatataacc attactcata cctggttttt ttaattgtac ggctgatata tttttaaagg
    36601 ggatagtttt ttctccttta ataccttgat taactaaatt tataaatcct tttctaataa
    36661 ttgaaataga atcatcagtt actttaatca aggtttttcc tggagatttt attaaaatct
    36721 ctttttccat tttttcttcc tcatttctgt gatatgattt tttttgtaga agctcatata
    36781 tgaggaaaga gcccgtgttg cagcacgggc ttttttaatg ttttgctgaa atcggttttt
    36841 ttaataattc ttgatgtatt catacatatt gccttgcgta agaatatttt ttcttaaaat
    36901 agcattggca gacagcataa caagtgtatc tgagcttatt ctgaatagaa taatactcca
    36961 taaatttttc gagattaaac tgagattcat cagtcagttc gttctcaata tagatattta
    37021 ataaaattag aatagctatt ttatctgctt ctgtttcaaa ttttgagtga aaagttgtag
    37081 aagtatcgta caaaactgaa aattcaaaat gagaggcgct gaaatgtgca agttcatgag
    37141 ataaatgaaa ggcttctgca gtttcaccgt atagattttc attcaaaaaa atgattctgg
    37201 gttttggata gtagaaacca ggctctttca tctccatata aactactttc aaattgtatt
    37261 cgctcaacat ttctttcaat ttcaaataca tacaaaccat cactccaact attcattttc
    37321 ctctaaagct ttagcaattg caatcgcttt acgcattgtc tccttagata tttcttttcc
    37381 atcaaaagaa aaaacagtat cgtcttctga taaatccaca tgtttagggg tttctctttc
    37441 ttctctacct agaaggtagt ctacagagac atcgaaatag tcagcaatcc gacttaattc
    37501 atctgaatta ggagtattat ttttccactt tgctagataa ccgttagaat atccaaaatt
    37561 aatttctaac tgccgtatag atatcttttt cctcttcgcc aagtctttta ttatttcata
    37621 ggtattcatc gataaaacaa cctttctaaa tgtttacaaa aaaagtttag aaaaatacgc
    37681 agaaataatt tgactaattc tgagtaatga gctatactat atcttgtaaa cagatttaac
    37741 aactaaaaag acaacaaaaa acactattga tatgtaaatg cagaccgcca agaaagcttt
    37801 aaaatcaatg tttttatgtc ttatttaatt atgctcttag tatagagtat tactcagata
    37861 ctgtcaattg aatttagaaa aaagttgtta aatttgttta cgaatataaa agaaaggaat
    37921 gagaaatatg aacacaccgc aaatttttaa tttcgaacaa aacgaagttc ggacaatttt
    37981 agtaaatgat gaaccatatt ttgtaggcaa agacgttgcg agtgttttgg gttattcaaa
    38041 cactaaagat gctttgtcgc ggcatgtaga tttggaagat aagatggggt cgcgaatcac
    38101 gacctcaggt caatcaagag agatgacaat catcaacgaa tctggtttgt acagtttaat
    38161 cttaaaatca aaacttccct ctgccaaaaa atttaaacgt tgggtaacaa gtgaagtgtt
    38221 gccagcaatt agaaaacatg gaggttatct aactccagaa aaagtagaag aagctttgct
    38281 taatccagat acaatcattc aattagcaac tcaactaaaa gaagaaagaa ctggaagatt
    38341 aatcgcagaa caaaagattg cagagtacga acctaaaatc tcctatttag atagcatatt
    38401 atcttctaca gattcagtaa caattagtca gattgcagca gattatggga tgtctccaca
    38461 acagatgaat aaattacttc ataaactagg tattcagaaa aaagtcggta accaatggtt
    38521 attgtgcaaa aaacacatga accaaggata cacaaaatct catacaactg agatcccgaa
    38581 agccgatggt ggcactaaaa ttgtaatgaa taccaaatgg acacagaaag ggcgtctatt
    38641 tatctacgaa ttactaaaaa aagaaggata ttaccctcaa atggatttag aggaaattgg
    38701 ttagaaagga gttttagtat gactgacatt gcagaaatca ctcaacgaga tagagaaaaa
    38761 atcaaagaat atgtcgaaag ttcaaagttc ttaacttaca ccatgcttgc tgaaagattt
    38821 ggaattagca aaagctactt atctttaatt ttaaacggta aaaagacttc tgcagaagca
    38881 aacagaatta tagattcgat tatcactatg tacgaattgt aaagggagga acagctaatg
    38941 caatatctag aagcaaaaat cccaattcca gaaggctatg taattatctc ccaagtggat
    39001 tatgaggagt taaaaaaagc tgatgatact ggtagatgga tgacgttgcc agaagtgcta
    39061 gaacggatta acagaaaata tgattggttt acttctagag ttttaaagaa cccaagatat
    39121 agaaatatta tcgatatcga gaaaaacaaa aatggatttg tctattatcc agttgaagga
    39181 agagacacat atctattttt aagaagtaaa acacttgaat ttttagaaac aaatttttcg
    39241 gaaatcttaa ggaggcaagc ggatgggaaa atttaataga gcgctagtgt tcagcgcacc
    39301 gctaatcatc tacgctttag gactttgggg aagcaggcaa gcgttgatag gaacgattgt
    39361 ttacatggtt tggattttta tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa
    39421 gccaaccgag ggggctgact aaaaatgaat agaaaagaga aactagaatg tatattacta
    39481 ttactcagtt taattctttc actaatttct ctattgggaa gtttgtattt ttgatatcaa
    39541 caattttgtg gatttgggtt ctatttagag taaatatcat ttttgtttgg tgattaagga
    39601 gtttattaaa attaattttc cctacttcaa atgcaaggta acaagatata gcacttttag
    39661 gaggaatatt tagcggtaca gcttcagagt atattctatt atcatcgact gatcctcctt
    39721 tagcaattaa tttcttaaat ggagtagctg accatctgcg gtttaagtta gcggatgaaa
    39781 gttctaaatt aactaacata gctggttctg ttgaatagtt agaaatgatt acttttgtat
    39841 aaatcatatc tgcagcgaag taagaagcat taagttcaac ttctagttga gggcgcattc
    39901 cttttatttt tatccctgtt aaaaacgtac tcaatataaa acttattatt gacatccatt
    39961 gaaaaattgt taagtgaaaa aaattcaaaa taaacaccac cagtttttaa ctaaattata
    40021 ccaaaaagga gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa agaagctgtg
    40081 actacgagct tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat
    40141 attgatgcac taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa
    40201 ccagattcat atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg
    40261 ctggctatgg ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca
    40321 ttcaacgaaa tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaagatatg
    40381 atgatctatc aattagaaga aatgaaagat gttaaaaaag atgtttccat gcttaaagat
    40441 actatgcgaa ttagcggaca acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt
    40501 atgggagttc tagggggaaa agaaagccga gcttatgaag aaatcagcaa aaaagtattc
    40561 tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc caagatatgg cgagttacct
    40621 cgtaagagat tcgatgatgc tgtttcattt attgaaatgt ggttgccaga aactgcgatc
    40681 cgtatggaaa ttgatcaact gaacagacaa cagagacttt ttggtgatga caatgaatag
    40741 agctgaagcg ctaagaatag ggacggcaat tgctaattgc tggtggaaat actataaacc
    40801 aatcatccta agccaacaac atattgacaa gcaaaaagca tggcaacaaa taaaaaagtg
    40861 actccgccgg caagcaaaga gtcacaaaga aaacacatca taaggagatt ttagcatatg
    40921 gaaaaagaac tttccactct agatcaatat ttgattgatc ctgattgggg caagccgaaa
    40981 attgaggaaa caagtggtcg aaaaatcaga cgaaatcttt tgacgaatga agaactagct
    41041 tgggatcaag atgatttagg caaccatgta actatttggg atcatgttta tcttatccat
    41101 ctatcgaagc attcgaataa acctgaatat atttacgtca tcgaagatgg cttgattgat
    41161 gcgctagaag agtacgacag agataacttg attgatatct cttattacgg accaggtaag
    41221 aaatacattg ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga caacgaactt
    41281 tgaaaaactt tttagtcgta agttaaataa aattctcaag aaaaaaggaa attttgatta
    41341 tttatcttgg gctcacgcgt gggagattat gaaaaagaat gatccacagg caacggtaac
    41401 tattaatgag tacaaacact acagggttgt ttctggaact catcaagact ttcttgttga
    41461 ggaatataaa ccttttctta tggatgaaac tgggacttat gtatctgtct cagtaacggt
    41521 taaaggacac acggaaaccg aattatttcc tgttttagat tatcgaaacc aaccagttgt
    41581 taaaccaaat gcaatgcaaa tcaataactc attgaagcga tgctttgtga aagcattggc
    41641 tctacacgga ctgggattat atgtatttca aggggaagat attccaacac cacctagaat
    41701 cgatacaaag aaattaaaca tgctagagac gattctagaa gctttcaatg agcagatggg
    41761 taaagatatg accaaaacct taatcgaata tgttaatgag cagacagata aattagggct
    41821 cttagctgat aacgttgaaa ctattgaaca gttaagctat gagcaatgtg ccttgatgga
    41881 gcgagcaata gcagctaaga gaaaagaatt agataagaag tgatatgagt gtttaaacca
    41941 ttaatcgatt catattcagc ggttctgaaa aagttcaaag gaaaagacat aagcgcaacc
    42001 atcaatgagg aagtgaacat tgatcgacta aagacgatgt atgacggcta cgatggtgat
    42061 cgagtcattg aaattcgttt tattgatcct agacgtttca ccgtacagca acgaaacttc
    42121 atctatgcgc tgataggcga tatttttatc gatacaggca tgccaacgga cttctggaag
    42181 gaattcttct actttcgttt tgaaggtgtc acagggcgca aaataagcct gaaagacgaa
    42241 tcgaatacaa ctgtgagtga tgccaatgtc ttagcaaata tcatcttaga tttcatcttt
    42301 gaacatcata ttcctttcaa agaaggctat gagattttac ctgcgaatca agaatattac
    42361 ttctacaaat gcatcacaaa aagagtctgc tgcatctgtg gcaaaacagg agctgacatc
    42421 gatcactttg acaaagcgct aggaagacga aagcgcaaag aagttgatca tgcagagtac
    42481 acatttgcag cactctgcag aatccatcac acagagaagc acaaaatagg tgtgattaat
    42541 ttcaaaaata aatatcaaat caaagggatc aaattaaacc agaaaacaat caaaaagtta
    42601 aatattggag ggtaaaaatg acagaacatc gaagttatta tgcgattata ccagccaacg
    42661 taaggtacga caaaagactt aaaccaaata ctaagttgtt atacggagag ataacggcct
    42721 tgtgtaatga aagaggcttt tgttgggcag gcaatgagta ctttgcagat ttatatggtg
    42781 tgaataaaga gaccatatcg cgatgggtaa gtgatttgat taagtttgga tacttgaatc
    42841 gggaaatcat ttacaaagag ggtaccaatc aaataatcaa taggtaccta cgaattaatc
    42901 aataccctat tgacgaaaaa cgcaataccc ctattgacga aaaagtcaaa gataataata
    42961 catctattaa taatacattt aataatacaa aagaatatat aagagagtta ccgccttcga
    43021 aaaaatcgaa ggctaagccc gtccgtcata aatacggaga gtataaaaat gttcttttgt
    43081 cagatgagca gatggagaaa ctcaaaacag aattccctaa tgattaccaa gagcgaatcg
    43141 aacgactgtc agagtattgt gaatcatctg gtaagactta taaaaactat ttggcaacta
    43201 ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa cgcaagcagt ggatacaagc
    43261 gcacaggaag acgagagaag cttcctgaat gggcaatcga ccaagaagcc tatctcaaga
    43321 aaaaagcgct agaacgagct aatagacaat caaaagcacc attctaagag gtggaaaaat
    43381 gaagatcgat tatctagaac taattaatga aatagcgaat tataaaaagg gcgaggaatt
    43441 agacgtcctg agagacgtat atgatcaact cgaagaagct ggaattgaag gaattaagaa
    43501 tgatcgttcg agttggagta agctcagata ctatttcgca ctttatatcg atacaacaca
    43561 attaagaaat ttagcatata ccaaattact atttgttgat tgtgttaaag gcttgcaaaa
    43621 acatcttaat gaacttgagc aggtgtaatc agatggacct aaagacattt acagcacaga
    43681 ttgaactaat gcatcaagaa gctttaagac aaagtgtatc gtacgaagac aagtggctca
    43741 acacgttcca tggcggacgt gagagcgcac ttgatcaagt actcaaatta ctgaaaggag
    43801 aatgtcggga tggataagaa agcggcaatg cagcgaatta tcgaattgac ttattcagaa
    43861 gattggcaaa atgacaaaga agctgcttca gaagtgatga ggcttggaag agcgatgtgg
    43921 gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa tttggcacgg tgacaaactt
    43981 ctagtgatag ggacagctga acagttagca agtctcacag gcttgcacga gaaaatcgtg
    44041 aggaaaagag caaggtgtgg ctacacagac gctaagaaga gaacgtttag atacgtggag
    44101 gaatcatcat gacaacagaa gaagtgattc aaatgcgtat tcgaagcctt cagcgtgaga
    44161 ttgacgatct ggaacgaaca aaggcagtga tggtcaatga aacggcgaga aaggcaatcg
    44221 atttgcacat agagaattta agaagggaaa tccatcgatt ggaggaatga gcgtggataa
    44281 gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca gaatcttggc aagaagacaa
    44341 agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg tggactgaaa agcccaaacg
    44401 gagaacgccg agaaaaattg caatctggca tgatgaccga attttagtaa caggtactgc
    44461 tgaacagtta tctgaaatta ctggattaag caaaaacatt atctgggata gagctaggag
    44521 cttatggatt gattcaaaag gacgacagtt taggtatgtg gaggagagat aatggatctc
    44581 attacacaat acagtgacat catcctcaag aaaatcatga tgaagattca gaaagataaa
    44641 aaatcaaaag aacgagctga attagttaag ttagaaatgg ctgaaacagg agcaggagtg
    44701 cgaagtagca ggcattggaa agcagcagca aacattgaat tttattacaa cgaaattcaa
    44761 aaagggttcg atcagatgcg tgagctggat cggcaaacaa attggagcaa gaaacttcat
    44821 caagatcgtt tcaaatttgt agagaagtat aaagaaatat tagaagagta tttgaggagg
    44881 acagcaaatg ataaaaaaac tcgttcaatt cagcatggat ttatatgata tcgaatcagg
    44941 agcaacacta tctgtggaat cggaccatct aatcataaat tttggtggaa agcgccagat
    45001 tattttgtgg gtagttgatg atgtactgtt tccagaaatt gttcatgatt tcgaagaatc
    45061 aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa ttgattgaaa aatacgagga
    45121 ggacagcgaa tgataccgaa gtttagagcg tgggataaac gaaagaacgt aatgagagat
    45181 gtagccgtct tgcattttac taaaaacggc aaaacaaact ttattgaata ttggataaat
    45241 cctaccgaat tgaaatcata tcacgtgcga aacatcgacc tcatgcaatc cacaggaatg
    45301 aaagataaga atggtgtgga tatatttgaa ggggatatag tattagtcag cgtgcgaaat
    45361 ggcttcgatt acttagataa taaagtctgt attgtcaaaa attcaataga ttattccgga
    45421 ttagtttgtg ccactgttga tgaagactta gagtatcaaa tttttaacac agagctgttt
    45481 gaagaataca cgtacgaagt caccggaaat atatacgaga atagcgagtt attggaggaa
    45541 cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag tattaaagcg ataggaaatg
    45601 atgcaatagc tgcttgctat aacgagagta taaattcagg tattacgtta atgaaaaaaa
    45661 tagacgaacc gcagaaacca gttattccac aactcgtggc cggttggctt gagaaatcta
    45721 cggacccttt tacaaaagct gaaaaaatag cgtatttaat caaatctaaa gatggtgatt
    45781 catattattt ctgtgattgg tttgtacgag atggcatagt gacgcaagag caaggagaag
    45841 aattactcgc ttgggcaaca agacaatcat atgaaacact attgagccta tacaacggct
    45901 acgaggttga gaaagagccg ttatgggcaa taaagaatgc cgatggaaac tatcttacta
    45961 aatgtgcttt atggggaaaa gatggagtaa attatagttt tgaatgcaat ccatctcatc
    46021 gattgctttt cactgataaa gcaacagcgg atgctgcagc attgttggtg aatggaacag
    46081 tggaagaggt ggtagaaaga tgaaactaaa agacggattt tacgctagca gtcacggtat
    46141 cggcggttta atgctagata tgccgacaaa gaaccctaaa acacgcaaga aatcaaaatt
    46201 caaagtcggt gacatggtcc gctgtgaagc agaagagttc gtttatccgt tcagaggata
    46261 cgttaaaaag atactgtcaa actcagcaat cattcgtatt gaaaatacaa tgaagtgtga
    46321 tcgttcgact gcaaaacaca agcattattt agctgtagcg agattagttg acatggaagt
    46381 aatcaagagc aaataaaaaa agccggatcg ctccgactga ttcaataaat ccaacacatt
    46441 tattatatca cataaaggag cggtttgact tgatgcaatt gttacgagag gtagatttca
    46501 aacagacaag atgtaatgcg agagatgtgc tgaagaactt tcggcgtttg gagcggatgg
    46561 caggtcgctc tttgatagat attaagtcgc cgattataac ggatatgccg aaggcaccga
    46621 agcatggcaa taaggcagaa gacgcgatta ttcagatgat ggatatagaa acggagagag
    46681 atgcgattct agcggctttg atggctctta gtctgattag tcgtcagata ctctactaca
    46741 gcttctgtgt gccagatagc ttctcaaact acagaattag ccgtgaagtg ggttattcag
    46801 aaagaagtat acaacggatg aagtcggaag ctctaataga gtttgcagaa gcatataaac
    46861 acggaaaaat aattgcttat aaataatttg gcggtttttt ggcggaatga tggcggtttt
    46921 tagacattta ccagtgttat tatggtagtg tcgaaagatt aggaaacagg atcgacaaaa
    46981 taaacgaagg gaggcaatct ccctcatcgt tttaaacttc ttcttagata gacagcaact
    47041 aattatgaag aaaaggatgt gaattcaact cctcctaaat tcttcatcgg ttgctgttta
    47101 ttttgattag acagcagcac tcacaaaact aaacatgttc ttgtcgtctt tcaactgctg
    47161 ctgtctatta atttatgtat tggagggaag aaaaatgaaa ttatcagtag aaggcactcc
    47221 agaagaaata caagaattgc tccaagctat tggtagtagc aaggagcaaa aggtaagtgt
    47281 ttctaattca gatattgacg gtatttgcaa ttgtttaaac agagaaaaaa tatctaatcc
    47341 ttactaagag aaaatatgtt cgttaatgaa tttccaattt tcctctgtag ctctaccttg
    47401 ataatttttt tgcagttcac agatgaataa ggaatcgtta ttatcaagta ctgtatttaa
    47461 tttttcacac atttgatttg gattcaaatt agttttaact aaccaaaaag atttttgtag
    47521 ctttatatat gccccaaatt cttttattat ttcaaatact ttatcatact tttgccctgg
    47581 gttattaaga tcataactaa tcatgtaggg tttatacatg ttgtcttgca catccttgtt
    47641 ttatatttca gcggaccact cgctgataaa taaaattata cgcttagtat ttattttcac
    47701 aatattaatt tatcgtaata acttttgagt aaatagaaca aaaaaactgc actagtttcc
    47761 gctagtacag gcagtgacta tatcggttga taatctagca tatattaaaa ataattgcaa
    47821 gaaaagaaat ttatagtggt ttataccaaa ttatcaaagc aatgttattt tgttgctgtc
    47881 tattgttttt taattattca cacgataact aaaggtgggt gaagagaaat gattccatta
    47941 ataatttcaa tttttgcgct ctgtcttaat gtctatatga ttggatttaa aaatgggcaa
    48001 aataaaaaat agtagcagcc aagaataatt ttatagtgtc actgtggcgg aaagggtaga
    48061 cgctaagcat gtgtgctagg tcaatgcttc ggcaaccatg caaggttcga ttccttgcca
    48121 gcgacattaa atgcctatga cggttacgac taccgaaaaa agatcgttaa gaagctatac
    48181 ggtgctacgt acggcaatgt agtaagtgtg ctatctgtac accaccaagc ttcggtcact
    48241 gtggcggaag tagaagacgc agcggtaaat ggcgagtagc ctcgtgagag cctggtaagt
    48301 tctcgtgagt ggtgcaatcc cactccagcg actttaagca accgaggcat cggcggttta
    48361 aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat cgtgcaagtt actattacat
    48421 attagatcac tctttgagtg gtctttttat ttttgcacaa aggaggaaac aacaatgtat
    48481 agaccacaat acttagaaca gaagtatgaa gtaatcactg ttcataaagg taatggcgaa
    48541 aaagtatatg agtatagaag accaataaag agcgatacat ataaacgaaa ggaaaacaat
    48601 gaagttattc cattgtatgg caaaagaata gctaagcatt aaataagatt gcgaaaggag
    48661 acggaacatg accgaggaat tctatagatg gctattacag ttgacaagag aagatcgttt
    48721 ggttaagttc tatcagtctc ctaaatggcg caggcttaga gagaaagcga tgaaacgaga
    48781 tcactatgaa tgccaagagt gtagaagact aggtaagtat catagagtag agaacgttca
    48841 tcatataaag gaagtcaagg atagacctga cttagcttta gatttagata atcttatttg
    48901 tttatgtgtt gaacatcata atgaagttca tggcagatat cttacagcat tagataaaca
    48961 agagaagaag atagaaagct ttgctaactt cgatgcaagt gaaaggtggt aagtgcatga
    49021 tcatcaatga taatggcaga gagtatgata cagaaaagat tgaagagtat tcatcttata
    49081 ctcagggatt aattaaacgt ttgatatacg ttcgctatgt aggtattagg gatctgttat
    49141 cagataactg ttgtagtaaa tacaaagtga atcaagtaag agaagcgttg aataaagata
    49201 ataacgtcga aagaataaaa aatgtttttg gatatagtat tgaagagatt aattattaca
    49261 ttgacttcgc tgaagctttc attccgatgg tgagataacc cccccttaaa ataaatcgca
    49321 aattttttgg gggtgatgaa acggaggggg ctgtcaggaa aagagatttt ttcgaacttt
    49381 atcatgaaag gagggctaaa atgtttaaaa acgaattgtc tcaaaatcgc tacagagaaa
    49441 aattacgccg ctctttaata agccaattgg aaagtcagaa aacaaatatt gagccattct
    49501 tagataatgt tgatcgttat atcagtttat gggaaacggc gatatcactg gaagaagata
    49561 tatccgagaa cggcattaga ctggagaatg gtaaaaagaa tgaatcagta gcgttgcttg
    49621 tttctgtcaa caaacaaatg ggattgatgt tggataaact tgccattact cctgaattgg
    49681 taggtgaagc aaatgaatca attcctgagt tataagcata ttgaaaattg gttcaaagct
    49741 atagaagaag gcactatcaa ggtatgcaaa gagcaattat tgctaaaaaa ttatctagaa
    49801 gaaagagtct ttactagaga agatatttac ttcgataagc agatggtaga ggattcaatc
    49861 aatataccag cacaatactt tccattcgaa ttaattccgt gggaaaaatt tctacaatgt
    49921 tttatttatg gtgttcgatg gaaaaaagat aaaacactag tgttcaatag atatctttca
    49981 ttaatgggac gtggtaatgg taaaactggt tttgcttctt ggaacaactt ctttctacta
    50041 accgctaaac acggtattaa aaattatgat attgatatct atgccaataa tgaaagccaa
    50101 gcaaagacta gttttgatga tgtatttaaa gtaattaaag atcatcctga tttagataaa
    50161 aaagtattta aagctacgaa ggaagttatt caaaatatcg ctacaaacag caaacttcgt
    50221 tataacacgg caaatgctag aacaaaagat gggaagcgac caggtgcaaa ccgctttgat
    50281 gaaattcacg aaaatgaaga ttattcaatg ataaatgtgg ctacttctgg tggtggtaaa
    50341 attcgagatt atagagaatt ttatgataca actaatggtc atgttcgtgg tggtccgctt
    50401 gatgacatta tagaagaatc aaaaatgatt ctttctggag aacttggaat tgacaaggat
    50461 ggagcagaat tttctagttt gtttccattt atttgtcgct tggataacga taatgaagtt
    50521 gatgatcccg acatgtggga aaaagcttgt ccaactatta attacaatgc agatttaaaa
    50581 cggaaaatgt ttcaagaata ctctcaaatg caacgtaatg ctggtttaag acttacgttc
    50641 atgaccaaac gaatgaacag acctatggaa gatacacgat ttgctgttgc ttcatatgat
    50701 gatgttctgc atacgaaaga aaaagaattt cctgaaaaaa tggatgaagt gataggaaca
    50761 gtcgattttg ctgatagacg agattttgcc agcgttgggt tgctaggaaa atacgataaa
    50821 gatgtttatt ttacacaaca tacttttatc cacgaatcag cccttcgatt acaaaacatc
    50881 aaacgagagg ttatagatat ttctatagat caaggaaaat cacagatcgt tcatggaaaa
    50941 aatatagaag ctgattatat tgtaggttgg tttcttgaaa tgagtaataa atattatatt
    51001 aaaaaaatcg ctatggatat gtaccgtgca aaaatattga agcccgcttt agaagaagca
    51061 ggttttactg tggaaattgt tcgaagcgga tctgttacac atggtatgtt aaaagatctg
    51121 gttgatgacc tttttattaa tcaacgttta ttttttggtg acgatgcgat tatgcgttgg
    51181 tattgcatga atgtatatga agagcatatt tctaatggaa atatacgcta tgaaaaaata
    51241 gaacctgaaa ctagaaaaac ggatggcttt ttttcattcc ttcatggttt gaatttttta
    51301 gatgatattt atgattctgc tcctgtaaca gtcacaaata gctcagtaga aaatacagga
    51361 actggattta ctcctctagt attctaactt gaaaggaggt gagaaagtgg ggatttttca
    51421 aaaggcggta ggatacttca caaaaaaagc aacggttcct ttagaagaat acttttgtaa
    51481 attgcaagtt gattttgtgt atcgaaaatt tgcgattgaa acttgtattg atttgattgc
    51541 aaatgcgatg agtaaagcgg aattcaagtc atatgaagat ggaaaaaata aaaagaatga
    51601 tctttactat aggctgaatg tagctcctaa taagaaaaat aatgcaacag aatttagaaa
    51661 aaaactgatc aggagattaa tattctacaa tgaagtattg atcgtttctc cgtctaataa
    51721 ttctagcgaa atatttattg cggatagttg ggatgtcaca gaatatgcat tgaaagatga
    51781 tgtgttttct caagtgcaaa ttaacaacat agtccttgat agagaatttc tagaaagtga
    51841 tgttatctat ataaaatacg cagatcaaca aattaggcaa ctagtcgatg cgtattatca
    51901 agcgtatggg aaactcattt ctagtgccat gaatgtttac aagcgctcta acgctcgtag
    51961 atacgtactg aaagggaatt tattccgacc gcaagacaat acaacacaag atcaaatcaa
    52021 taaaatgatg acatcacaat ttaaggcttt tatggaagct gataatgcag gtgcggtatt
    52081 tcaattacaa aatgagtaca cattagaaga tttcagcgga aacttccaaa gcaattcaag
    52141 agatataaaa aacttaatag acgacatctt tgagatgaca gcagcagcgt ttcacgttcc
    52201 gaaaaaccta ctaaagggag acatgagtgg gttatcggat caagtggacg cttttttaat
    52261 gttcgaaatc ataccgattg ctgaacttat tcaggatgcg tttaacgcta gtctctatga
    52321 agtagaagaa tacttgtcag ggaattttgt acgtgtggat acaactatga tcaagattac
    52381 tagcttcaaa gatttggttg acgctattga tgtaggcatt agaaatggag tatttacaat
    52441 caacgaagga agagaacgcg ttggaaatga tcgctctgat aaggcgatgg cagatgaaat
    52501 atttataact aaaaacaatc aacaagtatc gaaaggaggt gaggcgaatg acgacaatga
    52561 aaacatttct agcagtaaag aatgaaggcg cagtaccgca aatttttatt cagggattta
    52621 ttggttctag ttggttcttt gaagggaata ctgacaaggg aatcaaaaat attttggata
    52681 gtctaggtga tcaagaagaa attgaagtag taattaattc aaacggtgga gacgtatttc
    52741 aagggattgc tattgggaac ttacttaagt caaataaagc aaaagttaac gttgtgatta
    52801 acggcttagc cgctagtgct gcttcaatta tcgcaatggc tggcgatact ataaaaatct
    52861 acaacaatgc acaattgatg attcaccgcg cttccacata cggagaagga aatgtcgatg
    52921 acttccgtac gattgctgac caactggaat caattgataa atcggtaaag gcttcatata
    52981 aaacacgatt caatggcaca gatgaagcat tgcaagaact tcttgaaaaa gaatcgttta
    53041 tggatgcaga aacagctttg agttatggat tggtcgatga aattatcgat gcagaaaata
    53101 gctcaggtac tgaagctaaa aaagaacaaa gcgttgaaga aattttgaat gacgttaaag
    53161 aaaaaagagc agaaaaaatt gctgcattta cagcagcatt aaataaaaca tttggacaag
    53221 gagatgtaaa ataatgacag ttaaaaattt aaaaggtgta acagctgcaa gcgaccaatt
    53281 gatgaaagct tttaaagatg gtaacgaaga atcttttagc gcagctatgg taagcttatc
    53341 taaggaaatt caggataaaa ttttagaaga agcaacagca aaaaatcaag atcaattagt
    53401 attaatgaac cgtggtcagc gtgtattaac tacacaagaa acaaaattct ataacgaagt
    53461 agtgaaaaac gaaggttttg caggggtcga agaattagtg ccagctactg tatttgaacg
    53521 tgtatttgaa gatttagaac aatctcatcc actattgcaa aaaattactt ttgttaacac
    53581 aactggtgta acagaatgga ttgtgtcacg tggagtcaat ccagcatggt ggggtaaact
    53641 ttgcgaagct gttaaaaaag ttttagataa tggctttgat gtaattaaca tgaagcagtt
    53701 caagctatca ggttatattc ctgtatgtaa ggcaatgctt gatttaggtc cagtatggtt
    53761 agatcgttat gtccgtactg ttttagtaga atcattgaga attgcattag aacaagcaat
    53821 tgttgatggt actggtaaag atatgccagt cggaatgatg cgtgacatga gcaaacaaac
    53881 tagcggagaa tatgctgaaa aaaaagcaga acctattaca gctttagatg ctgtaactat
    53941 gggcggtttg atggcgcgac tatcaaaatt caatatcgaa ggtgtgaatg atccgattta
    54001 tcgtaatgtg aatccttctg atgtggtcct aattgtgaat ccaactgatt actggtctaa
    54061 agtattccca gctaagactg tactaactgc taatggagaa tacgtacaag tattgccagt
    54121 accagtttca gatttgcagt caacggctgt gccagaagga aaagcagtta ttggggtagc
    54181 ctcagattac ttcatgggtg taggatctac actaaaaatt gaagcttcag atgaatacca
    54241 ttttgttgaa gacgaacgca tttatctagc taaacaatat gcaaacggtc aacctaaacg
    54301 taacgatagt ttcattgtat tagatattag cgctttggga actactacta caactacaaa
    54361 accaacaacc acaacaacta caacacaagc gtaggtgatc agaatgaagt atattctttg
    54421 tcagccggca atcaatcggt ttaaatggga gcttgaagtt tgtttaacta atctgaagaa
    54481 actaggaatc aaagatatcg tattgctttt cagcagacac gatgatcaga ttcctatttt
    54541 ttttgagaag gaatatggtg ttgaagttca tgtgtacgat gatctgcggg acgacaaaga
    54601 gtatattcct tcgattaaac catatttatg gtggaaatat ttagaagaag atcattcgcg
    54661 tgaggacgac cgatatttct atatcgattc ggatgtcatt ttcaataaaa gaattaattt
    54721 gcgcaaattg ccttctaaag atgatgtttg gtattgtagc gactgctgta gttatctaag
    54781 tcttgattat attagaagct gtgaaaacgg agaaaatatt ctaaaagata tggcaaacat
    54841 tgtaaatgtt acagtagaat ctttggaaac tataaacact aattcaggag gcgcacagtg
    54901 ggttattaac cgtcctaaag cgaattattg gaaaaaggtt tatctggatt ctaatcggct
    54961 atatcgctac cttagagggc aaaaaacaaa tatacaaatc tggacagccg agatgtgggc
    55021 acagctttgg aacatgatgt atttcaatat tggtcctaaa gttcacgagg aattagactt
    55081 ttgttttgct actgatccaa tagaaaaagt taaagaagta aaaatcttac acaatgctgg
    55141 agtaacaaca aacgatgaag atttattttt caaagggaga tacgtgactt ctacgccttt
    55201 tgatgaagat ttatcatttg taaacaagaa aaaatgctct tacgcatatg ttaaagcaat
    55261 taaggcggtg gttagatgac gcctgaacaa gtgactgaag aattgctaat agctgtgaag
    55321 gataatattt acgttacctg gaacgaagaa gatgagtcaa ttaaaaagat gatagctaaa
    55381 aatgctgttt atcttcaaag taaagtgagt acaacacttt ctttttctcc tgaaagctta
    55441 gaatacggat tgctaatcga aagatgtaga tacgactgga atcgtgcttt agatgagttt
    55501 gaacaaaatt tcgctagtga gttattaggt ttcattcaac attatgcgct acaagaatat
    55561 attgcaggtg atgtgaatgg cgaataatcg tagactcgaa gaaacattca acgatggttg
    55621 gttaaagatt ttgacgcaaa ctaccaaaag aaatgaacta ggaaaaaaga ttggtgtaga
    55681 agatacagaa atcacttctt taaaatttag aaatctttcc atgagagata gtgatataac
    55741 agctatggat gcgatgggat cgaaattaac taagaaagta aagactccat ttcatccaat
    55801 cgccaagaaa tttaataaag atcaatattt tatcgtaatc gatagtatgc gttacaacgt
    55861 tatctatgcc gattacgata atttttatat ctatttttat cttgaaagtg tgggtgaata
    55921 tggtgattga taattctaaa gaaaaagaac gtttaaataa gcaaatttct gctatcaaaa
    55981 cttccttaga agagcatttt ggcctcaaac tctttcaaga ctccgtaggc gaggatgagc
    56041 tacctgatga ttttaattac ttcattctcg aaacaggaga aatagaaatg atcactgagc
    56101 caaaatatag cgtgggtcaa aatctatatc taactttcta ttcagaaaat agagaagatt
    56161 taacaggaga ctcactagat attatttcat tgattcaaaa tcgttcgatt cgttttcaga
    56221 gaatggatcc caatcattta aaactagaga accaagatcg ctatatcgat caattggtat
    56281 ttacgtttag acgattattg aagagtgatt gtcatggcta aaaatagttg ggagctaaaa
    56341 ataaatggac atgatgaact tcttgtgcgg atggaacgct attcaagcga gagcgaacga
    56401 ctgattaacg aagcattgaa atcaaaaggt tcggctattg cagtggatag gattacagaa
    56461 aaaattcctg tttctgaagc agatttaaga agagggcacc aacacgcaaa aaatagtcgt
    56521 ccacttaaga ctcaatacat taatttgggt ttcatcatta gacctacaag aaaatttgag
    56581 tatttaaaat atcctgattt ggggataggt acttctaaaa gaaatcagcc ggacgaattt
    56641 atgagaagag gattaggtct tgcacttgat ccaattacag aacttctgat tcgtcaattc
    56701 gataaattaa ataaataggg ggaacaacaa tggctaaaac aaaaactgta gtaacaacgt
    56761 tcgataacgt aagtatcaaa cgaattgctt ttaattttaa gaacgcagga aatgcaatcg
    56821 caacgaattg taatggacaa ttagatggcg aaacagaaat gcaaacggtg gttaaaaaat
    56881 gtggagcgac agaagtaaaa tcaaaatcta aaccaatcaa tatgacggta acaattactg
    56941 cacatgtacc gatggaagtt tatcgacgtt tcaatgggtt gaaacaagat ggacgtatta
    57001 aaccaggcat ttactcttac ggtcctgatt ccgtaggcga agatttctca cttgctgcag
    57061 agatcgtgga tgacttcgaa gaaaatagaa agttagttgg tatgttagca tgcacttcga
    57121 atacaggatt aacattctct attgaaaatg gtgcggatga agtagctgcg ttagaactag
    57181 aaacaaaagt tatgcaagat gaatttggta aattttatca tgaagcaatt gttgcagaac
    57241 ttgaagaaga cttaacagat caatggatga cgaatctatc tgctgatgtg attaaaaaaa
    57301 cttcagttgt gacaactacg gccactcaat cacagtaaaa aaaacggagg tagcgaaatg
    57361 aacgaagatt actcaaaaat tgaactaaac gatggaacaa ttttgaattt agaacctaaa
    57421 ctgaatatca agaaattatt gatgatcaat agagatttta acacagacga gtttgcaaaa
    57481 atgactgtgg gaaaaggatc catggatatt tctgttattc aaggtgcaaa ggctgtgtat
    57541 attgcttacc gccaagcgaa catgactgat tatatttcat tcgatgaatt tatcgataaa
    57601 tgggattttg atatggctac tgccagctat atttatcaat tgatgatgtt caaacaagca
    57661 cgcgatgctt atcaaaaaga atttgaaaaa gcaaataagg aaaaaaagct tcaaaagtaa
    57721 aaattccaaa gctcttagtt gaaacgtggg tcgatgtcta ttcgatgttg accgacgttt
    57781 tttctatgcc ttcagatttg gttttaagcg atatctgttt agatgacatt ttgcaaatgg
    57841 cttacaacaa gagtgcttat gaaggatgga aaaactatgc aataaaccaa tcccagaaaa
    57901 actaaagaaa ggaggtaaaa aatggctaaa aagagaacag aagcagaagt aactttcata
    57961 gctaacgatg acggattgaa atctacgtta aaagaaatca gtgctgaatt aactaaaaat
    58021 agagcagaat taaaactaga acaagctcaa ttacaacaga ctggttctga atcagacaag
    58081 ttaggaagta aattatcttc tttagaaaag cagtatgaat tacaaagtca aaaagttgaa
    58141 gtaactagcc aacgtttagc caatgcgaaa aaatattatg gagaaaattc caccgaagtt
    58201 cagaaacttg aaagagaact gattaatcaa caaacagcgc aacaacgttt gtcaaacgaa
    58261 attgataaaa cgagtaatgc actagctcaa gcaaaaggcg aaatacagac gtacgagtct
    58321 acaatgcaac agttggatag cgaacaaaaa aatgttcaag ctagtgcttc tctgattgaa
    58381 tccgaataca aaaaatggca agcaactgct ggtcaatcag cttctgaagc cgagaaatta
    58441 gcgaaagccc aagaatatgt ttctcaacaa tctgaaaatg cggagaaaac gatagatatc
    58501 ctaagacgac agttagaagc tacacagtct gaatttggcg ctacatccac agaagcaatg
    58561 cagatggaag cgaagcttaa tgatgctgaa cgtgaatttg aagagttagg acaagctgct
    58621 aaaaatgtag atacaactaa cttggacgat atcggaagca aaatagatat gaataattta
    58681 atggaagctt ctgacgtttt aagcgacatt ggcgataagc ttacagaatt agggaaacaa
    58741 gcagtggact ctgctaacag tgtaggtagt tcccagagta agatacaagc taattttggt
    58801 ttgtctaaac aagaggctga agaattaacg aatgtagcca gagacattta ttataaaggt
    58861 tttggagaat cgttagatca gtccacagat gcattgattt tggtaaagcg taatttagga
    58921 gatttaaata atcaagattt acaaaatatc acggaacaag ctatggtcct agaaaacacc
    58981 atgggcgctg atatggatga aacgttacgt ggtgtaaatg gcttaatggt caatttcggc
    59041 ttgagtgctc aagatgcaat ggatttaatg gtttcgggta ctcaaaacgg tttagataaa
    59101 acgcacgaat taggcgacaa tatggcagaa tatagccaat tatggagtca aatgggatat
    59161 tcagctgatg aaacgttcgg aatgcttcaa aatggtttag atgcgggtgc ttataacctt
    59221 gataaagtca atgacttagt taaggaaatg ggaatatcgt taacagatgg tcgatttgag
    59281 caaaacatgg atatgtttag tgaaagtact agaaaagctt ttgaagagtg gaaaaatggc
    59341 ggaggaacac aaaaagacgt tattaattcc atgattcaag attttagcaa tatggatggt
    59401 caatacgacc aattaaataa agcttcgaca atttggtctg cgcttggcga agataatgcg
    59461 atgaaagttg tccaatcttt aactgatgtt aaccatacat ttgatgatgt tagtggatct
    59521 gcacaaaaaa tgaatgaaga ttctactact ccgttgcaag agttgaacgg gaaaatagct
    59581 gaattaaagg attcattagc tcctataggc aacacaatca tagatgcact cgaaccagta
    59641 attgattttc taggaaagat ggctgatgcg tttaataatc ttccacaacc agtacaggat
    59701 ttcattgttg ctataggtgg tcttacagca gcatttggca tactgttacc ggcaatacta
    59761 gccgtgtcat atttatttgg tccgatgatg cttataattg gaggaattat agctgttata
    59821 gcaggcgtta ttgtggcaat taagaactgg ggtgcaatta ctgactggtt tagtggttta
    59881 tggaaaaaat ttactgattg gttgggtgat acttgggaaa gtataaaaga cggagcctca
    59941 tcagtttggg atggggttaa agaaacctgg tctggatttg tagattgggt tcaagatatt
    60001 tggcaaggag tttctgattg gtttggagag ctatggagcg gattagttga aggagcttcc
    60061 aacatctggc aaggagtcca agaaacttgg caaacattcg ttgattgggt ttcaaatatt
    60121 tggaacggag tcaaagaagt atggtcgatt atttgggcag acattgtagg aattgttcaa
    60181 ataccatgga cattaataac gtcattgatt caagctggta ttaatattat cgtgggtatt
    60241 tttgatgtag ctggacagtt attgggcgca gcttggcaag ctgtttggac acctatttct
    60301 gatttcctta aaaatacttg ggatactatg acacaatgga taagtatcgc ttggaatggg
    60361 attgtaacta cattccatac tatatttgat ccagtagtgg catggtggaa tggtatatgg
    60421 acagctatta gtactacggc ttcaaatatt tggaattcaa ttagtgcaac agcttctagt
    60481 atttggaaca gtatcaagaa tacaatcact agcttggtac aagcagctgc tacagtaatt
    60541 caaaatattt ggtcaactgt atctagttgg ttaggtggaa tttggaattc aatcagctct
    60601 acagcatcaa atatctggaa tagtgtgact agtagtataa gcaatgctat aaacgcagct
    60661 aaaagtgcca ttcaaagtgt ttggaatagt atatcttcgt ggattagcgg aatttggaac
    60721 ggtatcaaaa atactgcttt gaatctttgg aatggaatta caagcactat tagctctaaa
    60781 gtaaacgatg gaaaaaatgc aatttcaagc ggttggtcca atctaacagg tattgtttcc
    60841 gacatattca ataatgttaa aagtacaatt gctaacatat gggaaggcat caaaaagact
    60901 gttagcgctc caattgattg gattagagac aaaatcagtg gcatttttga taatttgaat
    60961 atttctatac cacatattcc gttaccggaa tttatcatgg aaggcagctt taacccgcta
    61021 aaaggtcaaa tcccccattt gcgtgtaaaa tggcatgcta aaggaggtat ctttactaaa
    61081 ccaactttac taggtggaat gaacggtgtc ggtgaagcag gacctgaagc agttttacct
    61141 ttgaaaagat ctgttttgca agaaattggt gatcgtatct tgagtagcac atcagtttca
    61201 tctagggcac aaacgattca acctgtgaat aactacgaat tcaatttcac aattgatggt
    61261 aacgcagatg aggttactat gaagcaaaca actcaacaaa tcattgatag cattacaaaa
    61321 attcaaaatg ataatgcttc ggcatggcgt taaacaggag agtatttctc ctgttttttt
    61381 agtattaaaa aggatgtgaa aaaatgactg attgtataca ttctataatc gatggatttc
    61441 ctgattattt gcataaattg gctttagctg aaagaccaac cataccttct ccaaaaagac
    61501 agagagttga aacttctgtt ttaggtaggt taggtggctt agtacaagat tactcgtttg
    61561 aagatatgtc gtttacattg cactataact atttagagga tgtggaagac catcaagcgt
    61621 tcaagcaatc gttttatatc atgcgtcatt ggttaaacta tgcaaagaaa ttagaattct
    61681 ctgacgatcc caacgtctat tacgttatcc agactatcga tattggggat gcagaaaacg
    61741 atattgttga atggggagag ttcgatgtaa atattactgc gaaaccattc gcaagagttc
    61801 aagaagatgt acctataacc gtagataaac cacagtcatt taacttgctg aataatagtt
    61861 tagaagaaag ttttccaaag attatcatca ctccttcagc tacttcatgc cagttcatct
    61921 taaatgatta tgtgtttagt ttcgaaggct tagtagcagg aactgatgta gtcattgata
    61981 gtgatttgat gctttgctac gaagagcaat cggacggaga tattttagat cggtccaaca
    62041 aaatgaagac catgcaatat ccgacattgc aagtggatat caatcatttt aattgcactg
    62101 gtttgagcaa aatacaaatt tatcgtaacg ggttaaggta ggtgaaatag atgatcgata
    62161 atttaataac tatttacgat aaaaatgacg cgaataattt agctgaacat ttatatgata
    62221 cgcaaggttt aggcgctttg tcagactggt taacagctac tgttagcaat aaactaaacg
    62281 gagccgagat atttcagggt acttattcaa taaacggaac taatgcagat ttgattgtag
    62341 acggacgtat tattcagtgt tatgtagatg aaaatcgagc aaaacagcgt ctacggattt
    62401 attatgcaaa aacttctgta ataggcaata cgatagaagt aaaagctgaa cctattttca
    62461 atgatataag aaaatcggtg ttgaataaat atgacagcgg aacagaaaag atcactgcta
    62521 ctcaggcatg gcaaaacgca aaagttttag cgaaaccagc tatcccttcg cagttttctt
    62581 tctcgtcatt agtagatacg cttgctaatg tgaagataga aaaggcgaat tttttagaat
    62641 tctttggtgg aaaagaggga tctattctag atcgatttca tggtgagttt ctaaaagata
    62701 ataacacatt acgtcatgaa aaaagtctag gtacggatca taaaatcaaa gcgatttata
    62761 ctaaaaactt aactggtctt gacttagaga ttgatgctca aagtgtttta gttggagttt
    62821 atccattcat tagcagctct tcagaaggag aagacgagat cactctacca gaagaagtta
    62881 ttttcacgga ttacgtggat gattatcctg ctggatatgt ttcttttgtt gattttaaag
    62941 acaaagcgac tgatgtagcc acattaaggg aagctgctaa agactggttg aaaacaaaca
    63001 tagataaaca aaaaccacaa gtgagtggtt cgattgaatt agtaccattg aggcatcaaa
    63061 gaggctatga aaaatttgtt gatctagaaa aagtttcgat gggtgacgga gtagatgtgt
    63121 atcatccaca gttaaaagtg aatatgtcag cgagaattgt ggaatatacg tttaatgttc
    63181 taaccaattc atacgataaa ttagttgtag gaaacgtcaa aacaaacttc ttagaaaaca
    63241 cagagaataa tgtcagcaat ttgattaatg atgccattga tcaattgaaa aacggtggcg
    63301 aaatcagtga tttactcaat gatattgtag atcatcaaac tgatatgatt actggtcaaa
    63361 atggtggtta tgttttatta gatcctaaag aagcgcctag tcgtattttg attatggaca
    63421 caccagataa gaataccgca aggaatgttt tacaaatcaa caatgctggt attggttttt
    63481 ctaaaactgg cattaatgga acatatgaaa cggcatggac gttagatggc ggattcaatg
    63541 cctcgtttat tacggctggt gaaatagtag gaattactat tagaggtact acattaatta
    63601 gtgatggtgc tgattataga acaagtattg ctaatggcaa aatgacttgg tactcaaaaa
    63661 aagttaacaa agatattatg gagctagaag cacgtgatta tgtaagtgct gatgccggta
    63721 ttgtatcata caccatgaaa actggtggtg gtttcatgat tagaaatcca cagggtaact
    63781 tggtttttag tacgtgggat aatggtaata acagaccgtt tctatctttt ggtgcgccca
    63841 atttcaggta tagcaatgct agttatgtaa cttctggcga cggtagttct ttaagcatta
    63901 atggtagtgc gggtaactca tgggaattta aggtagctgg taggactatg aaatttacta
    63961 gtgatggtat gctaacgtta ccaggttgtt tttttggttc atgggaagat gggaaacttg
    64021 ctaggtttga acaatcaacg gtacaagtat ataaagattt tactgttaga ggtactaaaa
    64081 actcaactgt accaacagaa cattatggac aacgactatt gaacgcttat gaaactccag
    64141 aatattattt cgctgattat ggggaagccg ttacaggtga caatggtaaa gttcgtgttg
    64201 atattgaccc catgtttgct gaaacagtaa atctaagtcg gtatatgaca catgtgacac
    64261 ctacagaact agttttgtgt gctgttactc atgaagatat tgaccatttc atcattgaaa
    64321 ctagtaagcc aaacgtatta gttagatgga atttagtggc acaccgtcta gggtatgaag
    64381 atattagatt aaaagaggat acagcatatg atagcacagt gcttgaccaa aaacgttttt
    64441 aaaacgaaga caaggaggta tataaatggc tagcagttta tataatttgg ctttagattt
    64501 cagcaaagaa ttaaactaca ccaaagctat tatggctcgt caaggtgata aagggattac
    64561 ggtgacggtt aaaccgtttc taaatggctt gcagatggat acgagtggcg gaacatttac
    64621 tttaaaagga acaacaccat ctaaccgtta cgtagataat gttgcaacta gtgtaactag
    64681 tgaagaagtc acgttttctc ttgatggcac atttatgagt gaagcaggat attataaaca
    64741 ctgctacgta gaatatagaa aagacaatca aattttaaca acgcaagata tcattttttt
    64801 ctcactagga gtgtctgaca tttcgcaagg ccaagccgat gaatatgttt cgcaattaga
    64861 agagttgatt cgaaagtaca acgaaacttt tgatgctttt atggctgaaa tcaaaggtag
    64921 agtggatagc ttaaatcaac agattactga tttaactggt caagctaaaa cgctacaaga
    64981 caagttagat gctctgaaag aagaaatttc taagttaggt aacttacaag tgatgtacag
    65041 taacagcatc gacttcgggg actatgatta ttcggagaac ccgaatttaa tgccatatat
    65101 tacagaacct tgggttggac ctttgttagg taatggacat acagttaaag atagtgttaa
    65161 aagggttata actcatacta aaactagaac agctaattcc ggcgacatat tatcgcttgg
    65221 cttaggtatt ccgtgtacag cggaagctaa taataggtat cttataacca ctttacgacc
    65281 atctacgaca tacacattaa gcgttactat gagtgtcggt tcagattgga ctggagagac
    65341 caatactata ggggttaggc ttagatattt aaatgaacaa ggtggtatag aactgcccat
    65401 taatgcatta ataccagcaa atgtagaacg cgataaaatg gtcactcata catttactgg
    65461 tataacaaaa gataatgtaa ctagcataac aaactgttat gttgaaatat tctcacttaa
    65521 tagtgaatat aaaggaacgg ttagtgtcag ctatgacgtt aagttaaaag cacactaccc
    65581 taacttacta gatggccctt actggttagg taaagttcct ttaggcgaga atattgctga
    65641 ccctacagtt gtctttccac acaaaactag tgaatatatg gtttatggta gacgcaatac
    65701 agaaaactat atcgctgacc aaacatatac catatcaatg aaagcaacta aactaacagt
    65761 tcaatcgttt gccgtgtata ttgctgctgg ccgcgttaaa gtaggagaca tgaagcctac
    65821 tgaggggtta gctaatacat gggagttaac ttttactgta actaagcaac atatagatag
    65881 tggggtaact aattatctgg aaatatacca atacccgtca gctacaaaag gagctgttca
    65941 aattgagtgg ctcaaactag aaaaaggcaa tactcgaacc ccgaatatta gtgagtataa
    66001 atatcgtggt actggtatgc gtgattcaaa caatccaaaa gattatgttt gggatctagc
    66061 accagaatat gtcgaagata atttggccac agatattaaa atttctgaaa ttactggtaa
    66121 agcaaacaat tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat
    66181 taatgaagta gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc
    66241 tatagatgaa ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt
    66301 aacagttaca aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc
    66361 tatatatttt gtggctttaa acggaaaaag cagtggtgga aatgattcaa cgatactaac
    66421 aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg
    66481 ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag
    66541 aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt
    66601 gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg
    66661 tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg attccaaatt
    66721 ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg
    66781 tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac
    66841 agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca
    66901 aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga
    66961 tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca
    67021 gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa
    67081 agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa
    67141 tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta
    67201 gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttc actaacgaac
    67261 aagtaatggt tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa atcattagca
    67321 aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg
    67381 attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt
    67441 ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa
    67501 gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg
    67561 atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatgc agaaaaattg
    67621 gtagaacaca cgcaacaaat taaaacttta tttagggaga gaagaaaatg aataataaaa
    67681 cgttcgaagt actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg
    67741 ggttagttgg taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg
    67801 gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct
    67861 cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa attactatta ctgcgatgag
    67921 cctgttaacg gctctttttt tattacccat taatacgttt gcttatacta ttaatgacga
    67981 gtataattta gcgccgaatc aaggagactc cagattagca attcctaaca aaattatttt
    68041 gcacgaaact ggaatagatg caccagcaag aaacgtagcc gccaacatga aaaataatta
    68101 taacggaagc aattcttata ctacagatgt tattggtgac ggtgggattg tttaccgtgt
    68161 gggtgagcaa ggatatgttt cgtggggagc tggtaacgct aatccttatg cgcctgtaca
    68221 gattgaatta cagcgcacat atgataaagc attgtttgaa aaaaactatc gagcttatat
    68281 tgaatataca agagatagtg caaaaaaata tggaattcca ttgactcttg atcaaggaac
    68341 ttctttattt acaaaaggaa tcatttctca tttgtgggtg acaaattatg tttgggggaa
    68401 ccacacagat ccatatggtt acttatcgca aatgggagtt agcaaagaaa agcttgctta
    68461 tgatttagct catggattta ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat
    68521 tgatccaaca cgagctggtg cagctaatcc tacgctgaca gatggaacaa atcacgccca
    68581 cattgatcag tttggggaaa tcgaaaacgc aaacttgcat gtggctggat ggcacattgc
    68641 taactataaa tacgagtata ttttcattat ggactacaat actgggaaag aattagctcg
    68701 agtaagagct gatggaattt atagaccaga tgtaaaccaa gcttataata cttctggaaa
    68761 tgttggttat catgtatctt tcaatatgcg taattttcct agtaagaaag tctatgtaat
    68821 gatgcgggca acgaatgatc cagagggaaa cactaaaggc ggtgcgcaag atttccatga
    68881 caaacgttgg tatttaaata ttcctaaacg ataaaaatag ctcctcgttg aggagcagta
    68941 cataactata ttgacaacta taaaaattat tcgataaaat agtgatgtta tcgcatatct
    69001 tcactatcac ccataaatag tcacactcca agctatgcga taacaggttt gttgccacac
    69061 attctactgg ttgattgttt atggctttat gt
//
LOCUS       DACCCR010000003        67606 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCCR010000003 DACCCR010000000
VERSION     DACCCR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.003"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            212..604
                     /locus_tag="IVK93_000182"
     CDS             212..604
                     /locus_tag="IVK93_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6385506.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IVK93_000183"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IVK93_000183"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP6385507.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IVK93_000184"
     CDS             3165..4775
                     /locus_tag="IVK93_000184"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP6385508.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IVK93_000185"
     CDS             5067..5936
                     /locus_tag="IVK93_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP6385509.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IVK93_000186"
     CDS             6101..7372
                     /locus_tag="IVK93_000186"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6385510.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IVK93_000187"
     CDS             7369..8664
                     /locus_tag="IVK93_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP6385511.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IVK93_000188"
     CDS             8763..9026
                     /locus_tag="IVK93_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP6385512.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IVK93_000189"
     CDS             9471..9920
                     /locus_tag="IVK93_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6385513.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IVK93_000190"
     CDS             9977..11395
                     /locus_tag="IVK93_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6385514.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IVK93_000191"
     CDS             11395..12255
                     /locus_tag="IVK93_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP6385515.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IVK93_000192"
     CDS             12429..13202
                     /locus_tag="IVK93_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6385516.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IVK93_000193"
     CDS             13349..13714
                     /locus_tag="IVK93_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP6385517.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IVK93_000194"
     CDS             complement(13862..15070)
                     /locus_tag="IVK93_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6385518.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IVK93_000195"
     CDS             complement(15089..15367)
                     /locus_tag="IVK93_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385519.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IVK93_000196"
     CDS             15560..16216
                     /locus_tag="IVK93_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6385520.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IVK93_000197"
     CDS             complement(16658..17455)
                     /locus_tag="IVK93_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP6385521.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IVK93_000198"
     CDS             complement(17452..18636)
                     /locus_tag="IVK93_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6385522.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IVK93_000199"
     CDS             complement(18661..19908)
                     /locus_tag="IVK93_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP6385523.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IVK93_000200"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IVK93_000200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP6385524.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IVK93_000201"
     CDS             21888..22202
                     /locus_tag="IVK93_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP6385525.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IVK93_000202"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IVK93_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP6385526.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IVK93_000203"
     CDS             22887..23531
                     /locus_tag="IVK93_000203"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP6385527.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IVK93_000204"
     CDS             23546..23875
                     /locus_tag="IVK93_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385528.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IVK93_000205"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IVK93_000205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP6385529.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IVK93_000206"
     CDS             24863..25687
                     /locus_tag="IVK93_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP6385530.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IVK93_000207"
     CDS             25680..26027
                     /locus_tag="IVK93_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP6385531.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IVK93_000208"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IVK93_000208"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP6385532.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IVK93_000209"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IVK93_000209"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP6385533.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IVK93_000210"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IVK93_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP6385534.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IVK93_000211"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IVK93_000211"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP6385535.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IVK93_000212"
     CDS             31684..32451
                     /locus_tag="IVK93_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6385536.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IVK93_000213"
     CDS             32441..34483
                     /locus_tag="IVK93_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6385537.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IVK93_000214"
     CDS             34764..34913
                     /locus_tag="IVK93_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP6385538.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IVK93_000215"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IVK93_000215"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP6385539.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IVK93_000216"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IVK93_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP6385540.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IVK93_000217"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IVK93_000217"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP6385541.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IVK93_000218"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IVK93_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP6385542.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IVK93_000219"
     CDS             39330..40412
                     /locus_tag="IVK93_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP6385543.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IVK93_000220"
     CDS             40412..41215
                     /locus_tag="IVK93_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6385544.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IVK93_000221"
     CDS             41285..42025
                     /locus_tag="IVK93_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6385545.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IVK93_000222"
     CDS             42036..43040
                     /locus_tag="IVK93_000222"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP6385546.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="IVK93_000223"
     CDS             complement(43085..44929)
                     /locus_tag="IVK93_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385547.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="IVK93_000224"
     CDS             45141..45659
                     /locus_tag="IVK93_000224"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6385548.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="IVK93_000225"
     CDS             45787..46470
                     /locus_tag="IVK93_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP6385549.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="IVK93_000226"
     tRNA            46779..46852
                     /locus_tag="IVK93_000226"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="IVK93_000227"
     CDS             47011..47370
                     /locus_tag="IVK93_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP6385550.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="IVK93_000228"
     CDS             47493..49124
                     /locus_tag="IVK93_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP6385551.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="IVK93_000229"
     CDS             complement(49186..49827)
                     /locus_tag="IVK93_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6385552.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="IVK93_000230"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="IVK93_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP6385553.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="IVK93_000231"
     CDS             50444..50788
                     /locus_tag="IVK93_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP6385554.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="IVK93_000232"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="IVK93_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP6385555.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51501..54335
                     /locus_tag="IVK93_000233"
     CDS             51501..54335
                     /locus_tag="IVK93_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6385556.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="IVK93_000234"
     CDS             54526..55017
                     /locus_tag="IVK93_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP6385557.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /gene="manX"
                     /locus_tag="IVK93_000235"
     CDS             55120..56109
                     /gene="manX"
                     /locus_tag="IVK93_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP6385558.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="IVK93_000236"
     CDS             56142..56945
                     /locus_tag="IVK93_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP6385559.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="IVK93_000237"
     CDS             56966..57880
                     /locus_tag="IVK93_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP6385560.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="IVK93_000238"
     CDS             57963..58337
                     /locus_tag="IVK93_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP6385561.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="IVK93_000239"
     CDS             58476..58721
                     /locus_tag="IVK93_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6385562.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="IVK93_000240"
     CDS             58893..59957
                     /locus_tag="IVK93_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6385563.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="IVK93_000241"
     CDS             60203..60628
                     /locus_tag="IVK93_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6385564.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..61070
                     /gene="nusB"
                     /locus_tag="IVK93_000242"
     CDS             60621..61070
                     /gene="nusB"
                     /locus_tag="IVK93_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP6385565.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61167..62021
                     /locus_tag="IVK93_000243"
     CDS             61167..62021
                     /locus_tag="IVK93_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP6385566.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62022..63359
                     /locus_tag="IVK93_000244"
     CDS             62022..63359
                     /locus_tag="IVK93_000244"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP6385567.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63364..63588
                     /locus_tag="IVK93_000245"
     CDS             63364..63588
                     /locus_tag="IVK93_000245"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP6385568.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63590..64480
                     /locus_tag="IVK93_000246"
     CDS             63590..64480
                     /locus_tag="IVK93_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6385569.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64489..65307
                     /locus_tag="IVK93_000247"
     CDS             64489..65307
                     /locus_tag="IVK93_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP6385570.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65414..65863
                     /locus_tag="IVK93_000248"
     CDS             65414..65863
                     /locus_tag="IVK93_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6385571.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65881..67563
                     /gene="recN"
                     /locus_tag="IVK93_000249"
     CDS             65881..67563
                     /gene="recN"
                     /locus_tag="IVK93_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP6385572.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
BASE COUNT        21847 a        11592 c        14366 g        19801 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    61081 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    61141 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    61201 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    61261 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    61321 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    61381 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    61441 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    61501 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    61561 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    61621 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    61681 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    61741 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    61801 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    61861 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    61921 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    61981 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    62041 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    62101 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    62161 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    62221 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    62281 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    62341 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    62401 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    62461 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    62521 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    62581 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    62641 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    62701 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    62761 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    62821 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    62881 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    62941 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    63001 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    63061 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    63121 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    63181 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    63241 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    63301 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    63361 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    63421 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    63481 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    63541 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    63601 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    63661 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    63721 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    63781 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    63841 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    63901 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    63961 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    64021 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    64081 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    64141 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    64201 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    64261 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    64321 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    64381 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    64441 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    64501 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    64561 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    64621 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    64681 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    64741 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    64801 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    64861 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    64921 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    64981 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    65041 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    65101 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    65161 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    65221 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    65281 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    65341 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    65401 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    65461 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    65521 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    65581 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    65641 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    65701 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    65761 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    65821 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    65881 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    65941 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    66001 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    66061 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    66121 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    66181 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    66241 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    66301 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    66361 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    66421 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    66481 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    66541 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    66601 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    66661 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    66721 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    66781 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    66841 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    66901 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    66961 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    67021 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    67081 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    67141 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    67201 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    67261 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    67321 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    67381 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    67441 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    67501 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    67561 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagct
//
LOCUS       DACCCR010000004        66048 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCCR010000004 DACCCR010000000
VERSION     DACCCR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.004"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(27..725)
                     /locus_tag="IVK93_000250"
     CDS             complement(27..725)
                     /locus_tag="IVK93_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP6385573.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IVK93_000251"
     CDS             complement(718..2070)
                     /locus_tag="IVK93_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP6385574.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IVK93_000252"
     CDS             complement(2293..3693)
                     /locus_tag="IVK93_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP6385575.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IVK93_000253"
     CDS             3854..4591
                     /locus_tag="IVK93_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP6385576.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IVK93_000254"
     CDS             4716..5195
                     /locus_tag="IVK93_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP6385577.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IVK93_000255"
     CDS             5192..5959
                     /locus_tag="IVK93_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385578.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IVK93_000256"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IVK93_000256"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6385579.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IVK93_000257"
     CDS             complement(6862..7464)
                     /locus_tag="IVK93_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6385580.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IVK93_000258"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IVK93_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IVK93_000259"
     CDS             8145..8729
                     /locus_tag="IVK93_000259"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP6385581.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IVK93_000260"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IVK93_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP6385582.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IVK93_000261"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IVK93_000261"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP6385583.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IVK93_000262"
     CDS             10630..10809
                     /locus_tag="IVK93_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385584.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IVK93_000263"
     CDS             10831..11856
                     /locus_tag="IVK93_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP6385585.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IVK93_000264"
     CDS             12031..13275
                     /locus_tag="IVK93_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP6385586.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IVK93_000265"
     CDS             13428..14375
                     /locus_tag="IVK93_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6385587.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IVK93_000266"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IVK93_000266"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP6385588.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IVK93_000267"
     CDS             complement(15277..15519)
                     /locus_tag="IVK93_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385589.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IVK93_000268"
     tRNA            15640..15712
                     /locus_tag="IVK93_000268"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IVK93_000269"
     CDS             complement(15831..16469)
                     /locus_tag="IVK93_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6385590.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="IVK93_000270"
     CDS             complement(16592..17224)
                     /locus_tag="IVK93_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6385591.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="IVK93_000271"
     CDS             17575..17874
                     /locus_tag="IVK93_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6385592.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18369..20534
                     /gene="acm"
                     /locus_tag="IVK93_000272"
     CDS             18369..20534
                     /gene="acm"
                     /locus_tag="IVK93_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP6385593.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20806..21291)
                     /locus_tag="IVK93_000273"
     CDS             complement(20806..21291)
                     /locus_tag="IVK93_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP6385594.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21771..22412
                     /locus_tag="IVK93_000274"
     CDS             21771..22412
                     /locus_tag="IVK93_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6385595.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22431..23066
                     /locus_tag="IVK93_000275"
     CDS             22431..23066
                     /locus_tag="IVK93_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6385596.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23084..23899
                     /locus_tag="IVK93_000276"
     CDS             23084..23899
                     /locus_tag="IVK93_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6385597.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24218..25654
                     /locus_tag="IVK93_000277"
     CDS             24218..25654
                     /locus_tag="IVK93_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6385598.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25843..28290
                     /locus_tag="IVK93_000278"
     CDS             25843..28290
                     /locus_tag="IVK93_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP6385599.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28520..29587
                     /gene="rlmN"
                     /locus_tag="IVK93_000279"
     CDS             28520..29587
                     /gene="rlmN"
                     /locus_tag="IVK93_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP6385600.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29780..30883)
                     /locus_tag="IVK93_000280"
     CDS             complement(29780..30883)
                     /locus_tag="IVK93_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6385601.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31436..31984
                     /gene="aac(6')"
                     /locus_tag="IVK93_000281"
     CDS             31436..31984
                     /gene="aac(6')"
                     /locus_tag="IVK93_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6385602.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            32530..34191
                     /locus_tag="IVK93_000282"
     CDS             32530..34191
                     /locus_tag="IVK93_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6385603.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(34261..34608)
                     /locus_tag="IVK93_000283"
     CDS             complement(34261..34608)
                     /locus_tag="IVK93_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP6385604.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            34692..35633
                     /locus_tag="IVK93_000284"
     CDS             34692..35633
                     /locus_tag="IVK93_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6385605.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            35634..36677
                     /locus_tag="IVK93_000285"
     CDS             35634..36677
                     /locus_tag="IVK93_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6385606.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            36683..37753
                     /locus_tag="IVK93_000286"
     CDS             36683..37753
                     /locus_tag="IVK93_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6385607.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            37743..38696
                     /locus_tag="IVK93_000287"
     CDS             37743..38696
                     /locus_tag="IVK93_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6385608.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            38708..39718
                     /locus_tag="IVK93_000288"
     CDS             38708..39718
                     /locus_tag="IVK93_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6385609.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(40003..40824)
                     /locus_tag="IVK93_000289"
     CDS             complement(40003..40824)
                     /locus_tag="IVK93_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6385610.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(40921..41199)
                     /locus_tag="IVK93_000290"
     CDS             complement(40921..41199)
                     /locus_tag="IVK93_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385611.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            41467..43476
                     /gene="metG"
                     /locus_tag="IVK93_000291"
     CDS             41467..43476
                     /gene="metG"
                     /locus_tag="IVK93_000291"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP6385612.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(43955..44542)
                     /locus_tag="IVK93_000292"
     CDS             complement(43955..44542)
                     /locus_tag="IVK93_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385613.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            44717..46039
                     /locus_tag="IVK93_000293"
     CDS             44717..46039
                     /locus_tag="IVK93_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6385614.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            46194..46970
                     /locus_tag="IVK93_000294"
     CDS             46194..46970
                     /locus_tag="IVK93_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6385615.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            46963..47532
                     /gene="rnmV"
                     /locus_tag="IVK93_000295"
     CDS             46963..47532
                     /gene="rnmV"
                     /locus_tag="IVK93_000295"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP6385616.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            47525..48409
                     /gene="rsmA"
                     /locus_tag="IVK93_000296"
     CDS             47525..48409
                     /gene="rsmA"
                     /locus_tag="IVK93_000296"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP6385617.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            48860..49489
                     /locus_tag="IVK93_000297"
     CDS             48860..49489
                     /locus_tag="IVK93_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385618.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(49613..50722)
                     /gene="ugpC"
                     /locus_tag="IVK93_000298"
     CDS             complement(49613..50722)
                     /gene="ugpC"
                     /locus_tag="IVK93_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP6385619.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            50931..51353
                     /gene="mgsA"
                     /locus_tag="IVK93_000299"
     CDS             50931..51353
                     /gene="mgsA"
                     /locus_tag="IVK93_000299"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP6385620.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            51676..52212
                     /locus_tag="IVK93_000300"
     CDS             51676..52212
                     /locus_tag="IVK93_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6385621.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(52252..54015)
                     /locus_tag="IVK93_000301"
     CDS             complement(52252..54015)
                     /locus_tag="IVK93_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6385622.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(54018..55733)
                     /locus_tag="IVK93_000302"
     CDS             complement(54018..55733)
                     /locus_tag="IVK93_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6385623.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(56512..56736)
                     /locus_tag="IVK93_000303"
     CDS             complement(56512..56736)
                     /locus_tag="IVK93_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385624.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            56936..59704
                     /locus_tag="IVK93_000304"
     CDS             56936..59704
                     /locus_tag="IVK93_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6385625.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            59785..60204
                     /locus_tag="IVK93_000305"
     CDS             59785..60204
                     /locus_tag="IVK93_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP6385626.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            60216..60686
                     /locus_tag="IVK93_000306"
     CDS             60216..60686
                     /locus_tag="IVK93_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6385627.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            60703..61482
                     /locus_tag="IVK93_000307"
     CDS             60703..61482
                     /locus_tag="IVK93_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6385628.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            61469..62290
                     /locus_tag="IVK93_000308"
     CDS             61469..62290
                     /locus_tag="IVK93_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6385629.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            62307..63356
                     /locus_tag="IVK93_000309"
     CDS             62307..63356
                     /locus_tag="IVK93_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6385630.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            63369..64373
                     /locus_tag="IVK93_000310"
     CDS             63369..64373
                     /locus_tag="IVK93_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6385631.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            65006..66025
                     /locus_tag="IVK93_000311"
     CDS             65006..66025
                     /locus_tag="IVK93_000311"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6385632.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        22103 a        11476 c        13457 g        19012 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat
    18181 aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat
    18241 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
    18301 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
    18361 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
    18421 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
    18481 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
    18541 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
    18601 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
    18661 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
    18721 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
    18781 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
    18841 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
    18901 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
    18961 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
    19021 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
    19081 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
    19141 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
    19201 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
    19261 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
    19321 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
    19381 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
    19441 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
    19501 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
    19561 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
    19621 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
    19681 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
    19741 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
    19801 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
    19861 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
    19921 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
    19981 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
    20041 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
    20101 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
    20161 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
    20221 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
    20281 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
    20341 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
    20401 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
    20461 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
    20521 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
    20581 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
    20641 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
    20701 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
    20761 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
    20821 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
    20881 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
    20941 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
    21001 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
    21061 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
    21121 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
    21181 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
    21241 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
    21301 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
    21361 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
    21421 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
    21481 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
    21541 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
    21601 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
    21661 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
    21721 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
    21781 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
    21841 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
    21901 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
    21961 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
    22021 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
    22081 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
    22141 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
    22201 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
    22261 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
    22321 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
    22381 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
    22441 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
    22501 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
    22561 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
    22621 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
    22681 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
    22741 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
    22801 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
    22861 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
    22921 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
    22981 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
    23041 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
    23101 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
    23161 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
    23221 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
    23281 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
    23341 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
    23401 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
    23461 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
    23521 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
    23581 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
    23641 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
    23701 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
    23761 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
    23821 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
    23881 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
    23941 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
    24001 acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc
    24061 aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt
    24121 tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa
    24181 gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc
    24241 atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg
    24301 gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac
    24361 atttaaagga acgaacggtg atttggcagt agaccactat catcgttata aagaagatat
    24421 tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat
    24481 ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat
    24541 cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc
    24601 acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac
    24661 tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt
    24721 gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt
    24781 gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt
    24841 catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac
    24901 gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga
    24961 cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat
    25021 gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct
    25081 agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt
    25141 caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc
    25201 agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac
    25261 aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag
    25321 ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac
    25381 agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc
    25441 catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac
    25501 agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca
    25561 agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca
    25621 aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac
    25681 ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa
    25741 aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac
    25801 ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg
    25861 cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa
    25921 atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa
    25981 ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac
    26041 caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag
    26101 ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta
    26161 ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg
    26221 acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt
    26281 ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga
    26341 agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa
    26401 ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa
    26461 ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc
    26521 gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca
    26581 gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag
    26641 gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa
    26701 aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca
    26761 gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt
    26821 gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata
    26881 ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa
    26941 caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc
    27001 gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta
    27061 acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca
    27121 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
    27181 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
    27241 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
    27301 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
    27361 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
    27421 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
    27481 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
    27541 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
    27601 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
    27661 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
    27721 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
    27781 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
    27841 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
    27901 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
    27961 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
    28021 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
    28081 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
    28141 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
    28201 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
    28261 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
    28321 taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt
    28381 ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat
    28441 ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga
    28501 gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt
    28561 aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg
    28621 gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat
    28681 cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc
    28741 ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt
    28801 actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat
    28861 cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga
    28921 aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg
    28981 cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat
    29041 ggactttttg catatcatca atgatgccaa aggattggca attggtgctc ggcacattac
    29101 agtttccact agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt
    29161 caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat
    29221 caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac
    29281 aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca
    29341 tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat
    29401 tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt
    29461 gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg
    29521 tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc
    29581 caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta
    29641 aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt
    29701 tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga
    29761 tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag
    29821 catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag
    29881 gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg
    29941 tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat
    30001 agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac
    30061 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
    30121 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
    30181 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
    30241 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
    30301 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
    30361 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
    30421 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
    30481 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
    30541 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
    30601 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
    30661 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
    30721 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
    30781 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
    30841 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
    30901 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
    30961 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
    31021 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt
    31081 ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct
    31141 aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt
    31201 taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg
    31261 ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag
    31321 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
    31381 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
    31441 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
    31501 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
    31561 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
    31621 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
    31681 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
    31741 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
    31801 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
    31861 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
    31921 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
    31981 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
    32041 aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa
    32101 caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt
    32161 gaaaatggct ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
    32221 acaccgttta ttcagataag ggactgtgac aagacttttg tcacagtttc tttttgttta
    32281 ttctttttta tctttgtatg gcaataatag acaatataac gaaaattatt taatggcttt
    32341 tggaagacca tggaaaacct tgtacaaagc tattttttct gaaaaattcc ttgaaacctc
    32401 acgaaaaaat cattatttta tgaaaaagac aaaaaaaagt gttgattttt taataattat
    32461 tcattaaact cttaaacaac ttcagaaaat attaattatt ctgaaaattc caataaaggg
    32521 gtacgtaaaa tgaaaaagaa atttacattt ggtttggttg cggtatgcgg ccttgtactt
    32581 gctggatgtt atggcggcag ttcagccaca agcagtagtt cagcaaaaag cggttcagca
    32641 gatggaggag gcgtatttaa cttagtcgtg cctcaagaaa tgcctacagc tgacttatca
    32701 gttgcaacag atacaatcag ttttacagca ttgaataacg tttatgaagg aatttaccgt
    32761 cttgatgaag acagcaaacc acagccggca ggagctagcg agttggcaga agtcagtgaa
    32821 gacggattaa cttacaaaat caaattacga gaagacgcaa aatggtcaaa tggtgatcct
    32881 gttacagcag cggattacgt ttatggctgg caacgaacag tagacccagc tacagcttct
    32941 gaatatgctt acctgtatgc gccagttgaa aatgcagaag acattacagc tggaaagaaa
    33001 gataaatcag aactagggat caaagcagtt ggtgactatg aattagaaat caaattgacg
    33061 aaacaaacac catatttcca atatctgctt gctttcccat catttttccc acaaaaacaa
    33121 tccgtagtgg aagaaaatgg ggatgcctat gcatctgcaa gtgataaagc tgtttataat
    33181 ggaccattca cattagaaga ttttgatgga ccaggaacag atacagaatg gacttataag
    33241 aaaaatgatc attactggga caaagataca gtaaaacttt ctgaaatcaa agtcagtgta
    33301 gtaaaagaat cttctactgc attgaactta ttcaaagatg gccaagcaga tgacgtgatc
    33361 ttgtctggtg agttagcaca gcaaaacgca aacgatccag cttatacttc tgttaaagaa
    33421 gcgcggacaa gctatatcga attcaatcaa cgtgaaaaga attcaccatt caacaatgtg
    33481 aacctaagaa aagctatttc ttattctatc gatcgtgaag ctttagtaaa acaagtacta
    33541 ggtgacggat cagttgtctc tacaggattg atcccatcag atatggtaaa aagtccaaca
    33601 acgaacgaag actttgcaaa agaagctgga aaattagttt cttatgacaa agataaagca
    33661 aaagaatatt gggaaaaagc gaaaaaagaa ttgggcgttg attctttaga atttgatttg
    33721 atggcttctg atgatgattc atcgaaaaaa gtaatcgaat atgttcaaaa ctcaatccaa
    33781 gaaaacttgg atggcgtaac agtcaaacca acacctgttc cattctctgt acgtcttgac
    33841 cgctcaactt cgggtgattt tgacgcagta ctaggcggat gggctgctga ctatgcagac
    33901 ccaagcagct ttactgattt gtttgtcaca ggtaattcat acaaccgtgg tcgctggagc
    33961 aatgcagaat atgacgaagc agttaaagca gcttcatcaa cagacgctgg cgatgaacaa
    34021 gcccgttggg atgatttcca aaaagcaatc aagatcatct ctgatgatat gggtgtcgtt
    34081 ccggtgtacc aaaaagcaga aggtcaccta gtgaatgaaa aaatcaaagg tatcgttcac
    34141 catgcggcag gcgcttcatg ggattacaaa tggacatacg ttgaagaata atcgttaata
    34201 ccgtgagcat gagaaaaaac gaggctgcgg caaatgtcgc aacctcgttc tcatattcac
    34261 ttatcctaac aaagaaaaaa gcatgaaaat agcagaaaga gcaattagta ttccggcaat
    34321 gatcagccaa aagctgtaca taccgtagct ggcagacgag agcgaggaaa attctggttt
    34381 tttctttcgg ttccttgttc gtgcaagatg gaccgatcgc tgaaaatgat cgaagaaacc
    34441 cgaagaaaag acccataaaa aaccgccgat aatcagaaac ggcaatgacc ataagaatag
    34501 atcattagat aattgaatga gtgtaaagga atggttgatg agatttttta ttaaaataac
    34561 cagaatcagt agaaaagcta ctgcgtaggg gatagatttt tttttcattt gattgctcct
    34621 ttatttttgg ttcattatct aataatgtag cgaaacatct gtcagaagtc aaaagctagg
    34681 gggaaacatt agtgaatagt tatatcaaat atgtattaaa acgtgtcttt ttcatgatca
    34741 tcacgctttg gttaattgca acaatcacct ttttcttgat gcaattgctg ccgggcacgc
    34801 catatacgaa tcaggaacgt ttgagtcctg aaacaatcga gatgctaaat aaacaagtcg
    34861 gtttggataa accagttatc gtacaatatg ggatttatct ttctaatttg cttcaaggag
    34921 attttggaat ttccttccaa tttaaaaacc agccagtcgc acatttgtta gctggaagaa
    34981 tcggaccatc cttgcagtta gggttacagg caattatctt tggaacagtc ctagggacga
    35041 ttttaggaac gatctctgcc atgaagcaaa atacatgggc agatacatct tctacactcg
    35101 tagcgatatt aggtcgttcc attccaaact ttgtatttgc tgttttatta caatatatct
    35161 ttgcaatcaa attacaagtt ttgccaatcg caaaatggga tggctttgtg tacacaatct
    35221 taccaacaat tgcgttggcc atgtcgccgc ttgctgactc tgctcggttt atccgtacgg
    35281 aaatggtgga agtactgcat agtgattatg tggaattagc aaaagcaaaa ggattgagcc
    35341 gttgggaaat cgcctttaaa catggtcttc gaaatagttt gattccattg atgactttat
    35401 taggaccgtt agctgtagca ttgatgaccg gatcactggt ggtagaaaat atctttgcta
    35461 ttcctggtat cggggaacaa ttcgttaagt caatcacaac aaatgattat cctacgatca
    35521 tggctgtaac gatcctttac tcatttatgc tgatttttgt tattttgatc gtggatctat
    35581 tgtacggtct agttgatcca agaattcgtg tttcagaagg gagccgagga taaatggaaa
    35641 tgataccaaa gcgttatgaa acagttgcag atatccctgc ggatgaattt cttccattac
    35701 aaaaaaatac cgaagaagaa cgtgaacaga ttactgcacc ttctttgaat tttatccaag
    35761 attcatggcg ccgtttaaag aaaaacaaag ctgccgttat ttcaatggtt ttactattgg
    35821 tcatcatttt gatctctgtg atcacaatct ttgtttctcc tcatgatcca actgcccaaa
    35881 acgtggctta tatcaatttg cctcctcgta ttccgggaat caatatcgat ggattgaatg
    35941 gaaaagcaat ggttggtggt gaacttgttg ataaatatgc tcaagcaaac gtcccttcaa
    36001 acgtaaattt ctttttagga acagacggtt tgggacgtga tgtattaagc cgcttattta
    36061 tgggaatgag aatctcgttg ctgatcgcat ttattgctgc attactagat gtaacgattg
    36121 gtgtagctta cgggttggtt tctggtttgt taggcggacg tgtcgataac gtgatgcaac
    36181 gttttttgga aatcttatca ggaattccaa acctcgttgt catgattttg atgttggtcg
    36241 tttttgaacc aggaattttt tcaatcgtgg ctgctatggc catcacaaac tggataccaa
    36301 tggcacgtat cgttcgtgca caaacaatga aattaaaaga tcaagaatat gttttagctg
    36361 gattgacatt aggtgaatca agaatgaaaa ttgcattcaa acatattttg ccgaatatct
    36421 ctagtgtcat tattattcaa atgatgttta gtattccatc tgctattttc ttcgaagcct
    36481 ttttaagctt tatcggttta ggtcttacgc ctccgtcagc ttcattagga acgatgttga
    36541 gcgaagggta caagacattc ctgtatctgc cttatctatt atggattccg gcagtaactt
    36601 tatcggttat catgattggc tttaacttac ttgctgatgg attgcgtgat gcctttgatc
    36661 ctaaaatgaa agagtgaaga cgatgacgaa aattttagaa gtgaaagact tagaaatctc
    36721 ttttgatacg tatgctggaa aggtacgagc gattcgcggt gtcaattttc atttgaataa
    36781 aggagaaaca ttggcgatcg ttggggaatc tggaagtgga aaatcagtga cgaccagaag
    36841 catcatgcgc ttgctgtcaa gcaacgccaa tatagatgct ggtcaaatcc tctttaaagg
    36901 taacgatatc gttggaaaat cagaaagaga gatgcaaaaa atccgtggta aagaaatagc
    36961 gatgattttc caagatccaa tgacctcttt agatccgacg atgcccattg ggaagcaagt
    37021 cgctgagtcc cttagaaagc ataataaagt ttcaaaaaaa gagggacaaa aagctgcatt
    37081 agaattgctt aaattagttg gaatacccga agctgagaaa cgtatcaaca gttatccgca
    37141 tcagttctca ggaggtcagc gtcaacggat cgtgatcgcg attgcactgg tatgttatcc
    37201 ggaaatcctg attgctgatg aacctacaac agcgcttgat gtgactattc aagcacagat
    37261 tttggagctg ctgaaagaaa ttcagacaaa aatcgataca tcaattatct ttatcactca
    37321 tgaccttggt gtagtagcga atgtcgctga tcgtgtcgct gtcatgtatg gaggaagaat
    37381 cgttgaagtt ggaacatcgg aagaaatttt ctacaacccg caacatccct atacttgggg
    37441 actgcttggt tcaatgccga cattagatag tgcgaacgat cgattgtatg ctattcctgg
    37501 ttctccaccg gatttattga acccgccaaa aggagacgca ttctatccaa gaaatgaatt
    37561 cgcgatgaaa atcgatgcag aacaagaacc accatttttt gaactatcaa aaacacacaa
    37621 ggcagcaact tggctacttg ctccgcaggc acccaaagtg acaccgccag aagaaatcca
    37681 acgtcgctgg gctatcttta aagagaaaca aaaacatact tacggtcaag gaggaattgc
    37741 tgatgccaaa taaactactt gaagttaaag gattgaaaca gtatttcaat gtaggaaaaa
    37801 agaatgaagt acatgctgta aatgacatca gctttcacat ttatgaagga gaaacattcg
    37861 gtttagttgg agaatctgga agcggaaaat ccactactgg ccgtacgatc attcgcttga
    37921 atgaacctac tgatggtgag atattattcg acggtcaaga tataacgaag atcaaagata
    37981 aaaaaggttt gacgaaattc cggcatgatg ttcagatgat ctttcaagat ccatacgctt
    38041 cgttgaatcc tcgtatgaaa gtcagagata tcattgccga aggaattgat gttaatggac
    38101 tagctaaatc atctgaagag cgtgcaaaaa aagtcgatga tctattgaga actgttggat
    38161 tgaatccaag ccacggtact cgttatcctc atgaattttc aggtgggcaa cgccagcgta
    38221 ttggtattgc acgagcattg gcagtaaaac cgcgttttat catttgtgat gaaccaattt
    38281 ctgcactaga tgtgtcgatc caagcccaag tggtcaattt gttgcaagat ctccaaaaag
    38341 aacatcaatt gacctatcta tttattgcac atgatttgtc gatggttaaa catattagtg
    38401 accgaatcgg tgtaatgcat aacggcttgt tattagagat gggaacaagt gatgaaatct
    38461 ataatcacgg tgttcatcct tatacagaaa gtttgttatc tgctattcct ctaccagatc
    38521 cagaccatga gagaaaacgc agaagaatca aatatcaacc tgaaccagat gacggacaag
    38581 tacgtcaact tcgtgaaatc gcaccagaac attttgtcta tgcaactgag caggaagtcc
    38641 catattacgc aaaaaaacta aaacgacaaa aggaatctct tctggttgct aactaggagg
    38701 gacatagatg ctaaaagctt ttaaattccg tatgtatccg acagaagaac aaaaacagca
    38761 attgattcgt acttttggat gtgctcgatt tacctataac catttgctga aaaagcggca
    38821 gaaaagttgg caacaaacag gagtagccaa tttttcgctc acacccgcta cattaaaaaa
    38881 agaataccca ttcttaaaag aagtagacag tcttgcttta gcaaatgcac agcttaattt
    38941 ggatcgggct tttcgcaatt atttcaaggg acgtgccagc ttcccaaagc ttaaaacgaa
    39001 aaaaagtatg tggcaatctt atacgaccaa caaccaaacc cgtacgatct atctggaaaa
    39061 tggctactta aagttaccaa aacaaaaaga actaatcaag ataaatcagc atcggccagt
    39121 tgaaggatcg attcgctccg ctacgatttc tgctcgatat aatgaagaat tttatgtcgc
    39181 actactttgt gatgtttcgc cagtaaaaaa agaatcttta gctaaatgga tcgggatcgc
    39241 ctatcatccg aagacattga tccaaacatc tcgacctcta gaagtaactt taccaaaatt
    39301 tcaccagaca gaagaaaagc tgcaacatgc acagcggaaa ttgaatgtga aagtccgctc
    39361 cgctcaccat agaaagatca ggttagatca ggcaagcaat tatcagaaac aaaagagaaa
    39421 agtaatggat ctttatctga aacaaaagaa ccagcgagaa gattatcttg aacaattatc
    39481 tgggaaattg gtcaaacaat atgactactt atttgtagaa tcttttccaa aagaagaggc
    39541 acatgctgat ttttctattc acgactggca taaattaata acaaaattgc gttataagtc
    39601 ccaatggtat aataaaaaat ttcttttgat caataccgac ggagcagaag aaagcaattc
    39661 cgtaagaaag agccaagtag tagaaaaaat gggcagacat tcggttatca agggataaaa
    39721 aatgaatgaa actgatagga gcgttagggt tagccttgtt aataaaaagt cttaatgact
    39781 tttgtttcta ggaatttttc ttttgaagaa aagctcacta attatgacaa accaataaaa
    39841 caatagcaga aaaagttcgt acgagctttt tctgctattg ttaatttttt attcagaaag
    39901 tttacgaata catcaaaatt ctgaaacaaa aaacgtaact cacaaagagt tgtttttgcg
    39961 ttttttcttg aatgattagg tctgaatctt ttgctcacaa ccttattcat ggtgccccgg
    40021 cttgagctga tagatttttg aaggacggcc gattcctaca ggacgttcac cgatctcatc
    40081 gaaatattgg agcatagctt tcttaaaatt ggaatgatcg atcgttttat aatcgatccc
    40141 taagaattta gcaaatactt ttcgagcttc cgtaatcgtg aaatctttac ctaaaacttg
    40201 tagaacttgc ggctcgtgtt ccattttatt gactacacgg ttgaatgctt tcaagatgat
    40261 ttgactatgg tcaaaagcca aagtatcttt gccagaagac tctccagtga ctaaatcaag
    40321 cgagatagcc acttctccgc tagataaatg gatcttgtta ctatgacgtt ctagtgtgaa
    40381 ccagcgtact tccttggcat catctccggc gattagcggt tcttctccaa taaaggctaa
    40441 gtagctgact gtgacgaccc agcctcgcgg atctcgatta ggcgtactga atgtatgcag
    40501 ttgctcaata ttttcttttg aaataaccac accagtttct tcctttgttt ctcttaagac
    40561 gctttctcca gttgactcat ccttttggac aaagccacca ggcaatgccc atgaatttcg
    40621 ataaggatgc ccttttcttt ggatcaacaa aactttcagc tgatcttctt ctttgttata
    40681 gcacatcagt acgatatcga cagtcaaaga gggcttgtca tattctggac gttcctgttt
    40741 ttgataccag ttaagaaatt cttcttcact tgctttatgt tcataatata atttttcttc
    40801 ttctttagaa gcaaaacggt tcatctgacc acctccattg acagtaaaag tactttttac
    40861 cttattgtag cagactctga aaaaaaacca aggatttact tgaaaaatct aggttatcat
    40921 ttaaatatga aaaataaatg gaagtatgag attgaataaa aagaaaagca gataaaacgg
    40981 caatacttta taccgctctt cacgaatggc aaagaagcca caaataacga tatatacgtc
    41041 aatgataaaa cgcaaaatcc aagctgctgt cataaaactg ttcaaatgcc catggaggtt
    41101 aaaataacca tacatacgaa tataaaaata gctgaatatt aaatgtgcta atgaaagaaa
    41161 agcaatacta agtgtaaaaa aaagatgttt cttctgcatg attgttccag tcccttacta
    41221 agatagatag tctttattat accacgtgtg aggggagaaa taaaaagccg ttttttagta
    41281 gatatgaaac aaaacaaaaa cccgcattta ttcaacctat tcatcctttt aaaggataga
    41341 ttgaacgtag atagaagtcg tttatccagt ctttttcatt ttgaaaaaaa gctaacagca
    41401 tgcttttttt atggtatact agcaaggaaa cgaattcgca tcatgcgata aaggaggaag
    41461 agccccgtgg cagaaaagga aactttttat atcactacgc caatctatta tccaagcggc
    41521 aaacttcata tcggcaactc gtatactacg attgcatgtg acgcaattgc tcgttataaa
    41581 cggttgatgg gctttgatgt gttttatctc actggagtgg atgaacatgg tcaaaaaatc
    41641 gaaacaaaag cagaagaact aggtgtaaaa ccgcaagaat atgtagataa aatggcggct
    41701 gatgtcaaga aattatggaa aacgcttgat atcagttatg ataaattcat tcgcacgaca
    41761 gatgattacc ataaagcagc tgttcaaaaa atatttgatc gtttattaga acagggagat
    41821 atttacttag gtgaatatga aggctggtac tctgtttctg acgaagaatt ctttacagaa
    41881 acacaacttg cagaagtgta caaagatgac gaaggtaacg tgatcggtgg taaagcgcca
    41941 agtggacacg aagtagaatt ggtaaaagaa gaatcatact tcttccgcat gagcaaatac
    42001 gcagaccgtc tattggaata ctatgaagaa catcccgaat tcatccagcc agaatctcgt
    42061 aaaaacgaaa tgatcaacaa ctttatcaaa ccaggattag aggatcttgc agtttcacga
    42121 acaactttta cttggggagt accagtcaag agtgatccga aacatgtggt gtatgtatgg
    42181 atcgatgcat tatccaatta tattactgca ctaggatatg gctccgaaga tgaaagtcta
    42241 ttcgaaaaat actggcctgc agatgttcag atggtcggta aagaaatcgt tcgctttcat
    42301 acgatctatt ggccaatcat gctgatggcc cttgatttgc ctctacctaa aaaagtattt
    42361 gggcatggct ggctattgat gaaagacgga aaaatgtcta aatcaaaagg aaatgtcgtt
    42421 tatcctgaaa tgcttgtgga acgttatggt ttagatgcac tccgttatta tttgcttcgt
    42481 gctgttccgt ttgggtcaga tggtgtcttt acgccagaag attttgtttc ccgtgtcaat
    42541 tatgatctgg caaatgatct agggaactta ttgaatcgaa cgattgctat gatcaacaaa
    42601 tattgtgatg gactcgttcc tgactatgcc tcacttgtca ctccatttga tagtgaactg
    42661 tctacgacgg ctgctaacgt tgtcggacgt taccatgatg caatggaaaa aatggaattc
    42721 aatacagcat tggctgaaat ctgggtgctg atttctcgtg caaacaaata cattgatgaa
    42781 acagaaccat gggtattagc aaaagacgaa gaaaagaaag ctgaattgaa tagtgtcatg
    42841 atccatttag ctgaatcttt gcgtatcgca gcgatattac ttcagccaat catgacagaa
    42901 acgccagaga agatcttcaa tcaactagga ttggactctg aaacaatgaa tcttgaagga
    42961 ttacactttg gcgaatttcc ttcaggaaca aaagtagttg caaaaggtac accaatcttc
    43021 ccacgtttag atatggaaga agaagttgct tttattcaag aaaaaatgtc tgaaggtact
    43081 caaacaaacg aagatacagt caaatgggat ccagaagaaa cagaacttgt ttcaacgaaa
    43141 gaaaaacaaa tcaaatttga tgtatttgaa aaagtagaac taaaagtagc agaagtcatc
    43201 aactgtcaaa aagtagaagg ggcagataag ttgctgcaat tccgactaga tgctggagac
    43261 agccaagacc gtcaaatctt gtcaggaatc gctgaatttt atcctgatcc aagcgaattg
    43321 atcgggaaaa aagtcgtcat tgtagcaaat ctgaaaccac gaaagatgcg tggacaaatc
    43381 agtcaaggaa tgatcctttc tgcagaagca ccagatggct ctttacaagt aatcgaagca
    43441 ccaaaatcca tgccaaatgg ctcagaaatc gcatagaaat tcaaaggggg tatgacacta
    43501 gttgtgtcat accccctttt tccgaaattt gagaagcaat tttcgtaaat tccttcttat
    43561 tacttgaagc caatcatata atacagaaac agagaactat ccaaaataaa tcctagaaaa
    43621 agaagtacga gcttcgctga tttttgttaa tttagaataa tactaatagg aatcgtaaaa
    43681 acagaaaaaa agcataatga taaagggtcg ggcagatttt aattgattca tctggtactc
    43741 ttttttattg tccttacatt cataaagttt gaatgcaatg actttattac atctatattt
    43801 atatctaaca gtagcgttga tgtgttaatt ataagttcta tcactttatg ttcaattagt
    43861 ttgtgctctt atatgtgtga ttgaaaatat agataaaaaa acaatcatcg gctttgactt
    43921 cgatgattgt tttctttatt ttgtaaaaat cctatcactt gattttatgg taacctttta
    43981 tttttgtctt caaacggctt ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga
    44041 cataccagaa aatcgtacgt ccgataccat aatgttgata gagctttgtc aacatcacgt
    44101 gtaacaagat caaccctaaa agcaaggagc cagctataaa ccaatgatta tcccaatgca
    44161 tgacttcact aagtgccatc actccaaaag gatagaaata aaaatagatc ccgactaagg
    44221 tcatccgcca gttctttgta aatgttccga tattcaataa aaagaaaatc gctgaaagca
    44281 aaataccgat aggcggaaaa agcccaacca aaaaggaata caacatcccc cacaagatca
    44341 tcacaggacc tttgatcaat gcaacgattg cgatgaacaa agtcagcaat attagttttt
    44401 gtgataacgt tgcaacagtc attaatccaa ataaaattaa tgtgaaccaa tgccaatggg
    44461 actgctgttg ttctttacgg gaaaaatcga cccggttgga taaataatct gctaatttcg
    44521 gataagaacg cttaaaatcc ataaactgtt cctccttcgt ggaaaaggta ggtttagtat
    44581 acgaaaaata aaaaagaaat acatccgtct taagagggag tttttcactg aaagtggaga
    44641 aaaaagaatg gtaaattaaa atattctcag ttatactgaa aattatacca aatttcaaag
    44701 aagaaagagg atgggaatga aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt
    44761 agctaccgtt atgggaaccg tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac
    44821 tgctagtact cagtctacaa gtttgacatt gctagcttgg cttttgggtg gtttcttaac
    44881 gatctgtgct ggattgacag tggctgaatt agctacagca atcccggaaa ctggtggagc
    44941 tgtaaaatat attgaagctg cgtatggaaa attgcccagc tttttattag gatgggcgca
    45001 aagcttgatt tactttcctg ctaatattgc tgcattatcg attatctttg cgacacaatt
    45061 gacgaactta ttacaattat ctaccgatta tcttttgttg attgcagtga tcaccgctgt
    45121 ttctgtgaca gggttgaatt tgttagggac aaaagtcggt gcttctgtac aatcagtgac
    45181 gttgattgtt aaattaatac caattgctgt gattgtcata tggggattat tgaccccagg
    45241 acaaggaact gtccagttat ttccaatcga agctggaaaa gatgttactt ttgtcgaagg
    45301 attgagcagt gcgttgttag caacgttgtt tgcttatgac ggctggttag gtgtcggtgc
    45361 aatggcaggg gaaatgaaac ggccggaaaa agatttgcct aaagcaatta ttcttggttt
    45421 gagttttgta acagtcgttt atctattgat caattttgtt tttttgaaaa ctttgccgat
    45481 tgatcatctt gccggtaatt tgaatgcagc ttctgaagca tcagacgtca tttttggagg
    45541 aatcggtgga aagctggtca ctattgggat cttgatctcg gtatacggtg cattgaacgg
    45601 ttatacacta actgggatcc gcgtgcctta tgcgatggct ttagaagacg agctaccttt
    45661 cagtaaacag ctgacaaagc tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt
    45721 ccagttagca gtagcttgta ttatgatgag ccttggttct ttcgactttt taacagacat
    45781 gttgattttt gtgatgtggc tattcagttt actgattttt atcggtgtct ttattttaag
    45841 aaaaaaagcg ccggaattgc cacgtccata taaagtacca ctttatccaa tcgttccgat
    45901 tattgcgatt ctcggtgcta ttttcatttt aggtatgacg atgatgacgc aaacgaaatt
    45961 agcattgatc ggtataggtg ttaccttgat aggtatccca gtctattatc aaaaaaagaa
    46021 aaaacgctca gaagagtaaa atatttcaac aacagacaga aaaagtacga actaggtcct
    46081 ctagatcttc gtgctttttc tatttcaata aaacaagttc ttctattttg atttttgcta
    46141 cttctgcaag tagtgcttat gtgctaaagt aagaatgaaa aggagagata aacatgatat
    46201 ttgattctca tacccattta aatgctgaac agttcaatga agatattcca gaaacgattc
    46261 agcgagcaca agaaatgggg gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga
    46321 ttgaaaagtc tttgcaatta agccatgact atccaaatat atacagtatt atcggctggc
    46381 atccgacaga ggctggcagt tatacgaaag aaatcgaaaa taaattacaa gaacaattga
    46441 ctttaccaaa ggttgtagca ttaggggaaa ttggcttgga ttatcattgg atggaagacc
    46501 cgaaagaagt ccaagataaa gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc
    46561 taccaatcag tattcatact agagaagcac ttgaagacac gtatcaaatc ctaaaagaag
    46621 aagatgttcg agatatcggt ggaatcatgc atagtttcag tggtgattat gaatgggcaa
    46681 aacgatttct tgacttaggg atgcatattt catttagcgg tgtcgtcact ttcaaaaaag
    46741 cattagacgt ccaagaggct gcagcaaatg taccaatcga acgattactt gtggaaacag
    46801 atgcccctta tctggcacca gttcctttta gaggaaagcg aaatgaacca ggctatactc
    46861 gttatacagt agaaaagatt gctgaacttc ggaatcttcc gacagaagaa gtggctgccc
    46921 aaacatggac aaatgcgcac cgattatttg gtctaagcga acatggataa gatgaagatc
    46981 caagagatta tagtcgttga aggaaaagac gatacaagaa ggctgcggga agtagttgat
    47041 gcagatacga tagaaacaat agggtctgca atcaatgaag agatcctgat gcaaattgaa
    47101 catgcccaag agactcgtgg agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa
    47161 ataagaaaaa tcattatgga agctgttcct caggcaaaac atgctttttt gccgcgcaca
    47221 caagctaaac caaagaaaaa gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt
    47281 ttagaagcat tgaaaaaggt cgtgacgcca gacatttccc aaacggctgt ttctgaaatc
    47341 actagacaag accttttaga ctatggttta atagctgggg cacaagcaaa agaaaaacga
    47401 gaaaaactag gtgacgagtt gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt
    47461 ttgaagatgt ttcgtattac gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa
    47521 caacatgagt gaatatcgtg aaattgcgac accctctaga acaaaagaga ttttattgaa
    47581 acatggtttt tcatttaaaa aaagtttagg acaaaatttt ctgactgaac caaatatttt
    47641 aaggaaaatc gtagaaactg cgcaaataga tgaacatacg aacgtgatcg aagttggacc
    47701 aggaatcggt gcattgacgg aacaattagc caagcatgca aaacaagtcg tagcatttga
    47761 gatcgatgat cgtttgatcc ctgtattggc tgatacgatg caaccctatg acaatgtgac
    47821 tattgtacat caagatattt taaagaccga tttgtcaaca gctgttagag aaacatttca
    47881 tgaggagctt ccgttaaaag tcgtagcgaa tttaccttac tatattacga caccgatcat
    47941 gatgcatttt cttgaatcag atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga
    48001 agtagctgat cgtatctctg ctgaaccagg aacgaaagct tatggttcat tatcgattgc
    48061 tgttcaatat tatatggaag ccagtttagc attcatcgta ccaaaaacag tatttgtccc
    48121 tcagccaaat gtagattcag cgattttgaa actgacaaga agagatatac cggcagtcga
    48181 agtgacagac gaaaaagcat ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa
    48241 aacgctttgg aataacttgc agcattcgta tggcaaagat gaccagacaa aagcatggtt
    48301 agcaaaaagc ttggaaacag ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt
    48361 acaagaattc gccgcattaa gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt
    48421 gtgacagaag ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
    48481 agttcctcat gtttttcgtt tgtttgggct tattaccgca gatgcaagct tttgaacacc
    48541 gtttaagaaa gaggttgtgg gcctgccgtt taactctaag tcacaaggaa caatttcaaa
    48601 aaacagcttc tcatgttttt gagttagttg gtcttattac ttgatgcaag cttttgacca
    48661 ctattatgcc gaacaaggag atgtgacaca aattttgtca catctcctat ttttgtttct
    48721 tgatcaaaaa attcttatac ttgatactca cactcatttt gtatggattt agaaaagaat
    48781 atgtgtcttg acagttatta gtaagtgagt tatactaagt cgacatttat gtgtcaagac
    48841 atattaggag agagaaaaaa tgagaaaaaa ttcagttcga aaactaacga tttctgcttt
    48901 attgattggg atgggggtca ttatcccaat ggtcatgcct aaaatcatga ttccaccagc
    48961 ttcctttact ttggcgtctc atgtgccatt atttattgcg atgtttttta caccaggagt
    49021 tgcagtggct gttgcgctag gtacgacatt tggtttcttt ttaacaacgc cggttatcat
    49081 cgcattgcga gcattgtcac atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa
    49141 gcatccgagt atcgttttaa aaaatggaca gtttactttt gctaattggc gtttccaagg
    49201 gttcaatttt gtgatcggtg tgattcattc tttagcagaa atgctagtag tcagcatctt
    49261 ttactttatt ggtaatatgc ctgaaacata ttattctcaa ggatattttt atacagtatt
    49321 catcttgatg gggattggtg ggcttatcca cagtttagtt gattataata ttgcttattt
    49381 tatagcagga acactcagca aatattttga tatccccgta tttacggcag caaaaaaaga
    49441 aaatcggaaa gagtcgattc aagggtacgc gaagaaaacg acatattaga catgatctct
    49501 caaaaaaaaa actctcatct agtaatggat aataaatcct ctaagatgag agttttttgt
    49561 cctaaaaaga gctgcaccaa catcttctgg cacagctcta acagggggca atttatggaa
    49621 gtttgatgac ttgttctgtt tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa
    49681 ggtcgactat ttcgcctggt ttatggaaat ctcttgcatc tacttttgag atgaactcag
    49741 tagaaccagt ttttgtgtaa agcatcgttt cagctccaag taattcggaa acgacaactt
    49801 ctgatttcac tacggcttca ggtgaagctt caagggctac ttgttcactg tggatgtctt
    49861 ctggacgaat accgaagata acttcttttc cttcataacc tttttcaact aatactttgt
    49921 tttttccttc aggaatacga agcttaagcc catgtccatc tgtgataaca ccatttttca
    49981 atgtcacatt aaagaagttc atggcaggag atccaataaa gccggcaacg aacatattga
    50041 cgggtgtatc ataaacttct ttcggtgtac cgatttgttg gataaatccg tctttcatga
    50101 taacgattcg gtcagccata gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg
    50161 ttgtttcaag acgctggtga agtttggcaa tctcagcacg catggctaca cgtaatttag
    50221 catctaaatt tgataatggc tcatccatta agaagacttt tgcatcccga acgattgccc
    50281 gtcccaatgc cacacgttgg cgttgtccac cagataaagc agctggtttt cgtttcaagt
    50341 attctgtcag acctaagatc tctgctgcat tgtccacacg ttttttgatg tctgctttat
    50401 catatttacg taatttcaag ccaaaagcca tattatcaaa aacagtcata tgaggataca
    50461 atgcgtagtt ttggaaaacc atggcaatat cacggtcttt aggtgccacg tcattcatga
    50521 cttttccgcc gatatttagt tctccttcag agatatcttc cagtcccgcg atcattcgca
    50581 aagtagttga ctttccacaa ccggaaggac caacaaaaac gataaattca cggtctttga
    50641 ttgatagatt aaaatcagtt acagaataat tttctgcatt gtcatatttt ttatatacgt
    50701 ttttcagagc catttctacc ataatacgtt ccacactttc ttttttagtt ttattaacta
    50761 tttaaagtat aaatgaaagc gttaactaac ttcaatgtca tgttgaacaa gaattttttc
    50821 ttttttttgt caatgtgcta gttttaagct ttaataaata tccataagaa ataaaaactg
    50881 atacactgga gtaaataagt atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg
    50941 ctttgatcgc tcatgaccga aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa
    51001 caatattgga aaaacatgaa ctttttgcta ccgggacgac tgggcagagg atcacggaag
    51061 caaccggctt gccgatccat cggtttaaat ccggaccgtt aggcggggat cagcaaattg
    51121 gagccatgat ttcggaggat cagttagatt tggtgatttt tcttcgagac ccgctggctg
    51181 cacagcccca tgaaccagat gtaacagcac ttatccgttt atctgatgtc tatgagattc
    51241 cgttagctac gaatatcgga acagctgagg tgctactaag aggactggaa gccggcttcc
    51301 tagattttcg aagaatcgtc catgaaatag acaagcatcc actttcattt tgatggataa
    51361 gcgtatggaa aaataacgtt taatgttcgc ctatatgtag ctataaaagc taaacaaact
    51421 gataatatgc gatataatac gaacgtattg gataatataa acaataatat tagtttttcg
    51481 tatttgcagt aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc caaaaataat
    51541 acagagtaga aaataaagcg ttaagtgcta agaggatggg atgttgcctc ttggacgaag
    51601 gacgtaatgt tcgcggtttt attttcgcat tcgctgcatg atacgatgta gcagctctta
    51661 aaggagatgc taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc tattatgggc
    51721 ttattgattg cattgatggt cactttgtcc cgactggtag caatcgagac cccatttatt
    51781 aaaatcagtg tcacatttat tccacaagtg atcatgggca ttctttttgg tcctttttgg
    51841 tcagggatag gcgcagtttt agctgattta gtaggaatgg ccctcttctc gaaatctgca
    51901 ttctttattg gatttacatt gaatgcattt attgaaggag caatctacgg attctttttc
    51961 tatcgaaaag agatcacttg gaaaaatgcc attctggcta cgcttagtgt cacgctgatc
    52021 atcaacttat ttttgacacc attatggctt gcgttgatgt accatgtgcc attgttcagc
    52081 tgggtcgttt gggcgccccg cctgttaaaa acagtaatat ggttaccgat tcaatcaatt
    52141 gccatttatt acgtaggacg tagtattcct tacaaaaaaa ttttaagaag tttagcgatc
    52201 cacgctaaat aaaaaacaag cagtcttccc atcccagaag actgcttgtt tttattgtgc
    52261 tgtgaattgt gcttgataga gccgtcggta ataaccatct ttttgaagca gctcatcatg
    52321 ggtgcccatt tcgacaatag ctccctgatc catcactaat atctggtccg catttttgat
    52381 tgtggacagg cgatgggcaa tcacaaaact ggtttttcct tccatcattg ctaagaaggc
    52441 ttgttggatt tttttctctg tcaatgtatc gactgagctg gtagcttcat ctaagatcaa
    52501 cataggggga ttactgatca tcgttcgagc aatcgtcaac agttggcgtt gtccatctga
    52561 tatctttaac ccttgaccgc caatcttagt atctaatttt tccggcaaac gttctacaaa
    52621 ttcatacata taagaggctt ttagggcatc attgatttct gcgtcacttg cttccggatt
    52681 cccaaaacga agattttctc gcaatgtact gtcaaatagc caagtatctt gtaacaccat
    52741 tccaaaactt ttacgtaatg tatcacgttg gatagagcgg atgtcatggt catcgactgt
    52801 aattgttcct tttgttgtgt cataaaaacg catcagcaaa ttaacaagtg tggattttcc
    52861 ggcccctgtt tttcctacta tagcaatggt ttgacctgga taagcagtaa aattgaaatc
    52921 ttcgatcaaa cgctgcttgg ggtcataaga aaaagaaaca tgatcaaaag agacttttcc
    52981 tttgatattt ttgggttcca aggcataggc atctggcaaa tcaggctttt cttctggctg
    53041 atcgatcaaa tcaaatgctc gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat
    53101 tccagaaagt tcgataaatg gtttagaaaa ctgacttgag tagatcgtaa agctggaaat
    53161 cacaccaaca gtgattgacg aaccactctt caatgccaat aggccgccaa ctaaaccaat
    53221 cgataaataa gcaaggtgat ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa
    53281 ttgtgcccgt tgtccttttt catatagatc agcatttagc agttcaaatt gctgttgact
    53341 agcttcttct tgctgaaaag ctttcacgat tttctggtta ccgattcgtt cggtcacaaa
    53401 gcccgagata tccccaataa tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc
    53461 aacgatccaa ctcacaagga aaataattgg tgtagaacaa agaacgacca atgtcagaag
    53521 tggacttaga cgcatcataa aaataaacga aagaatgatg attgccactc cagaaaataa
    53581 ttgattgaag gccgcagaaa ttgcaatgga aatattgtcc atatcatttg taaatcgact
    53641 gacgatattt ccgtgggagt tttgatcata ataacgaagt ggcaatcgat tcagatgagc
    53701 aaatgcatct tttcttaatt ttgcaacgga taagtaagct aaccgattgc ttaacacttg
    53761 gatgagccat tgactgacta cgttgattaa aacgatacca ccgaatagaa taagtaaatg
    53821 gaacagtcga ttgaagcgga cctgtccttc tccaatcatt tgatcgatac tttgaccaat
    53881 ttgtaacgtc atcacaactg ttgcaatccc attgatgatt cctagtatca cagctagcca
    53941 aatgtctttt gcataggcag taaggtaagg aatgaatcgg cggagcgtct gcaaggataa
    54001 tttttttact ttcataacta ttcttcctcc tgtgatgcga cgatttcctg atattctgct
    54061 gattgagcaa gtaattcttc atgggtaccc tgagcagcaa tcttaccttg atccatcact
    54121 aagatatgat gtgcttcttg aatagagcgg acacgttggg agataataat cactgtactg
    54181 ccaagataag tctgtagcgc cttacgcaaa tccaagtctg tttgataatc caacgcactt
    54241 aaagagtcat ctaaaatcag taatgctggc ttggcaatca aagctcttgc aatcgtcagt
    54301 cgctgttttt gtccgccaga gaaatttttc ccattttcca tgacttttgt atctaaacca
    54361 tttggaagct gacggacaaa ttcttctgcc tgagcgattt tcagagcttc ccaacattct
    54421 ttttctgtgg cgtctttctt accccattga aggttttcac gaatagtccc ggtaaatagt
    54481 acagaagttt ggggaacaag agcaatttgc tgacggagat gatccagctg ccaagaacga
    54541 acatccattc cgtccattat aactgtaccg gaactgacat catagaaacg cggaatcaat
    54601 tgtgtcaaag tcgttttccc gcttccggtt ggaccaatga ttccaaggac tgtatttcgc
    54661 ttcagctgaa atgtaatatt ttggagagaa agaccagaat caggagtata acggaagtca
    54721 acatggtcaa aagcgataat tgcttcagaa gaaaaatcgg gagtagtact gacctcagct
    54781 tcaagaatcg aattttctgt gtctagcacc tctttcaccc gatttcctga agcttcggca
    54841 cgtgtgaaga tcacgacgag gtttgaaaca acgatcagtg ccaaaagcat ttgattcata
    54901 taattgatca aagcaagaac ttgtccttgt tgcaagctac caatatttac tttgatcccg
    54961 ccaactgtca gcaaacagat gatcccgaca ttcatgatca aggttgtagc aggtgataat
    55021 agggcagaga tattggaaac acgtaaataa ttgttcgcta gatcatttgt gcttttatcg
    55081 aacttctctg tttctttttt tgtctgcgca aatgcgcgga tgacacgaac accgctaaga
    55141 ttctgactaa cgtgttcatt gagctgatct agtttttctt gcactttttg gtagagagga
    55201 acggtttttt tgatgataaa aaataaaatg attgagaaaa taggcaataa tgctaagaaa
    55261 aacagaccta cttcccagtc gatcacaaaa gccatgacca ctgacccgat acttaaaaac
    55321 ggtgctctga tcaataaacg aatgaccatt gctaaagcta gctgaagctg attgatgtcg
    55381 tttgtcattc gggtgatcaa ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa
    55441 gtattgattt ttttgattag ctgattacgt aactctgtac caaatccttg tgaagctact
    55501 gaagcatagt attggcaaat gatagcacaa atcagaccaa tcacagacat agcgaccatc
    55561 cataagccca tttcaatgat ttttcctcgg tcattcattt ttagtccatt atcgatcaag
    55621 gaagccatca ttaaaggcag gacaagttcg aagacagctt caagaaattt gaagaacggg
    55681 cccaaaatga tttgcttacg atagtctttt gcataacgta ataactgaat catatgattc
    55741 ctcctattat ttatttgtcc acaattgaaa aaacaagtac taccacattg tagcttagga
    55801 aatttagaat gaaaagagaa caagcctttt cataaatgaa agttttatta tgaagaagct
    55861 ttagaaattg aatagactat tttttttaaa aaaaagaaaa tgagaaaaca tgccagtctc
    55921 ttcattcata ggaacgtcta ttttacttaa tttttctata aatgaattct gcataaaaaa
    55981 tatatcaagt aaatgtttat agatgaaact ctatttactt cttttttaat ttgttcttgc
    56041 aatctttttt gctacgtaat ttttgtaaaa aagtgggttg agagaaatct aattgatttt
    56101 caaagaagga gtatgaaaat gagaaaaata tttattcgtt tattgtgggt actatcttag
    56161 aggaataaat aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga
    56221 acgaagacaa cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa
    56281 aaaagaaaac aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa
    56341 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
    56401 ctgaaagcat cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata
    56461 taaggatttg taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt
    56521 atggaaggat ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc
    56581 tatatattgc gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa
    56641 aggcaaaaat acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga
    56701 agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc
    56761 tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa
    56821 aattataagg gataagtagg ctgctgtcta taataagata tatttttcaa ttctttatta
    56881 aagattttat tgacaaaatg aatccgtttt ctatgatgga ggaggggtga ataagttgtt
    56941 gaagaaggaa attttatgtt acttagagaa ccagacagcc tttttcgatt tagaacatat
    57001 gaacgaggtc tttacagcac aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt
    57061 cagtcattat ttgaaccagt taaatgatga gcaactacta gtcaaaatca attctcgacc
    57121 agtgatttat ttccacaaag aagcttttga aaaacaattt tttaagttac gcacgaattt
    57181 ttatttttct atcgatgatc tcaaagagga acagcctttt ttcgcgcaac caaaagactt
    57241 attctctttt atgatcggat acgacgccag tttgaaagaa agtatcgaac aaatcaaaac
    57301 agctctttac tatccagatg gcggattacc acttttgatt acaggcgaaa gtggcacagg
    57361 taaaagttat ttagttaatt tggtctatca gtattgtctg cttcatgatc tgctagaaga
    57421 ttctgcacca ttcatcactt ttaattgtgc acaatatgca gataatcctg aacttttgac
    57481 tagcaatttg tttggacatg ttaagggagc gtacactggt gcagaagaaa ataaaaaagg
    57541 ggcttttgaa gcagcagatg gtgggatctt gtttttagac gaggtacatc gcttaacacc
    57601 agaaggtcag gaaaaactgt tcacctatct tgatcaaggt gtgatttatc gtatgggaga
    57661 acctaatttg tcacgccgga tcaaaacacg tctctttttt gctacgactg aagagattac
    57721 tagtcatttc ttaacaactt tcattcgaag aatacctatc cgaatcgaac tacctgcact
    57781 taatcagcgt agtcgtaatg aacggctaga actgatttac tctttttttc tcgaagaaca
    57841 aagaaaaatg gggagccctt tgtcagtcag tggaccagct ttgtcacttt taacaagtca
    57901 gaaattatcc ggaaacatag gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc
    57961 atatgctgaa cagcgtgaac agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga
    58021 actacttgcg cagcctatta ctaaaaatac agcacagcaa ggaatctata ttacgcaaga
    58081 ccagacctta gagcaattaa tggagaagca acagcccgaa cagcagcgaa tcgttaaaag
    58141 ttttgagcaa atcctaattg atttcaagaa aaataactgt ctgctatatt cttgcgaagg
    58201 aaaattaaag caagaagtga ttcaattatt tgattttttg ctgtttgaga ccagtagaca
    58261 agaaaaacac gagctgctgg tttattggac acaatctatc cgagagacct ttcgacaaat
    58321 ggaaactgcc tatcaaatca aattcgatgg aaacagtgtc tatgccttga gttactatct
    58381 ctatcagcga agcactatca aatggttacc ggaagaacat gatattatcc aactaatcaa
    58441 agaattagaa caacaagttt ctcagtctta tccatctagt tatcactatg tccaacgaat
    58501 tctggaatta agtaagatca aattagatat tgaagacaca agtatggatc ggattttact
    58561 gacgatctat ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat
    58621 tgctgctcac ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc gtttattaca
    58681 agaggatcta tttgaatcat ttgatatgcc actagatgtc acaccacagc agattgcttc
    58741 agaaatcctt gattattgcg aaaacagcga tgtttcaaat ggattgatca tattagtaga
    58801 catgggatct ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg taccgctttt
    58861 gattttgaat aatgtgacga cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa
    58921 tgtatcatta gaagagatag cagaagagac tgttcgacag atccagccag aatggcgact
    58981 gctctatcca gaggaaaaca aacccaaagc actcattact acttgcttca ctggaatcgg
    59041 tacggcggtc cacttaagtg aactgctaga aaaaagctta cctacggctt gtcagttgaa
    59101 aatcatccca tatgaatatc agcaattaaa agataagaaa aacagtgacc cattattttc
    59161 catatacgaa gtcgtaggaa tgattggcac cacagatcct gatatcacca atatcgacta
    59221 tctttcatta gaagacctca tctcggggga aaagatgtct gtactagctg aatggttgga
    59281 cactactatg aatgcttcag agaaggaaat attcaatcag cgcatcattc gtaatttttc
    59341 attggaaaaa gtactggatt ccgtgactat tctagataca gagaaggtga tgggagaaat
    59401 cgatcatttt atgagagaat tagaggtgca gttgaatcga agcatcagca atcccaaaaa
    59461 attagcgttg tatgtccatg tgagctgtct cattgaacga ttgatccgac aaatgccaat
    59521 cgaaacctat caaggattag agaaatggaa gcagtgtcaa aaagaagggt tatctgctat
    59581 taaatctgcg tttagtgtca ttgaagagca ttatagtgtc atgatcccca attctgagat
    59641 tgcctatatc tatgatattt tgagtgaaga tacagagttt ctctcgatag aagaagaatt
    59701 ttgaacataa gcaagttgtt gccactaatt ttggcatgca acttgctttt ttagtatgtg
    59761 taagtgaaaa gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa
    59821 tttgcttcag ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact
    59881 gtattcgatt gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt
    59941 atgaggtaca aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac
    60001 aacgaattct tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg
    60061 ccactattga ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc
    60121 cagcaggtgc taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag
    60181 aaaatagaag aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc
    60241 atcggttatt acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta
    60301 ttttgattgc taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag
    60361 ccaagccaca aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga
    60421 aaagcggggt gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat
    60481 ataagctggc agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa
    60541 aacctggtac tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct
    60601 tgaaacgatt aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa
    60661 aagtggatgt aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc
    60721 ttattaggaa ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt
    60781 tctcgtccga ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg
    60841 atcattatgg gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg
    60901 attccacctg atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga
    60961 gccggtacag aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg
    61021 aaaaatattt acttagggct tttgattcca attatgaacc ataaagcaga caattatgca
    61081 gaagaaggaa attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc
    61141 ttgatgcttg gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat
    61201 ctattaaata tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt
    61261 ccagctcttg gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat
    61321 tttttcttag gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt
    61381 tttggtgcga ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt
    61441 caaacaactg caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat
    61501 aacgaaaaaa gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa
    61561 ttatgaacgt cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt
    61621 atatcagtcg aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac
    61681 gaccccacat atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa
    61741 ggatgatcct gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc
    61801 tcttgcaggg ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt
    61861 gggtacctct ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt
    61921 caatattccg cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac
    61981 gggttttttg aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc
    62041 tattattgga ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc
    62101 tttaaaaatc ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt
    62161 tccaaatatc ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt
    62221 gaaaccatta acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg
    62281 gattttttaa aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa
    62341 aggaagtact aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa
    62401 aaacagtgaa tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga
    62461 agccagcatt ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt
    62521 tcaattatgg aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga
    62581 aaagtgcttc tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg
    62641 ctatcacaaa tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag
    62701 gtgccggggt agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt
    62761 tactactctt agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac
    62821 aagactacat gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa
    62881 cggaagaatt ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca
    62941 ttggatatgg tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa
    63001 tccgcgttcc gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg
    63061 cagatgcttc gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc
    63121 aaaagttgca aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa
    63181 aagcaagaaa cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat
    63241 tagcgcttgt cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg
    63301 acttgaacaa aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg
    63361 tggaaatcat gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag
    63421 ctttacgtcc gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg
    63481 tctattatct aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga
    63541 atgggaaaag caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa
    63601 acaaaagatt aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag
    63661 aagtagtgaa agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg
    63721 ataaagcgaa cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg
    63781 gaacagaaca aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat
    63841 tcgaagctta ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg
    63901 aagcagagaa aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata
    63961 gtatgcatta ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt
    64021 gttactggga agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc
    64081 acgggacgtt agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg
    64141 aacagagagt gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata
    64201 cattgataga cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg
    64261 ttttgtcttt cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt
    64321 tgaactgcca tccattagag atacgccgct actatcgtca gtttgattat taggatagac
    64381 tggataaaaa gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta
    64441 aaatattggc atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta
    64501 taatctgttt aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt
    64561 agttgttgca tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag
    64621 cgttttgtat ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac
    64681 aatctgtccg ttgcaggacc tgacagattt agaaatgttc aatctgtctc ttatgtattt
    64741 taaatcaata ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca
    64801 ttgtcaaaac tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt
    64861 agactattgt aacatatcat tgattatttc aagtaaaaac gataaaagaa gtaaacaaac
    64921 acttaaaaac aatggaaatt agtcccaact ttaagaaatg tatactataa tggaaattat
    64981 caattattta tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc
    65041 ttggggcact gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg
    65101 aaatgtacct gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc
    65161 tgatttgacg atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa
    65221 cgcagatgct attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact
    65281 tattggtaaa atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga
    65341 tacgcataaa cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc
    65401 aattgttgta ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac
    65461 aattacagct gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa
    65521 tgattatttc aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact
    65581 gaaaaatatc attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc
    65641 aaaagcagca atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg
    65701 tgccaatcct ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac
    65761 aagtgttcat tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga
    65821 agaagttcta gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt
    65881 agagctagct caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt
    65941 gtataatgga gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat
    66001 ggagaatgaa ttttctcttc gttaaataga tgatcaatag ttaatcgt
//
LOCUS       DACCCR010000005        64270 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCCR010000005 DACCCR010000000
VERSION     DACCCR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.005"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(212..1603)
                     /locus_tag="IVK93_000312"
     CDS             complement(212..1603)
                     /locus_tag="IVK93_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP6385633.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1816..2202)
                     /locus_tag="IVK93_000313"
     CDS             complement(1816..2202)
                     /locus_tag="IVK93_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP6385634.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2258..2881)
                     /locus_tag="IVK93_000314"
     CDS             complement(2258..2881)
                     /locus_tag="IVK93_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP6385635.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2945..3613)
                     /locus_tag="IVK93_000315"
     CDS             complement(2945..3613)
                     /locus_tag="IVK93_000315"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6385636.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3625..4065)
                     /locus_tag="IVK93_000316"
     CDS             complement(3625..4065)
                     /locus_tag="IVK93_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385637.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4124..4795)
                     /locus_tag="IVK93_000317"
     CDS             complement(4124..4795)
                     /locus_tag="IVK93_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6385638.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4980..5378)
                     /locus_tag="IVK93_000318"
     CDS             complement(4980..5378)
                     /locus_tag="IVK93_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP6385639.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5375..6148)
                     /locus_tag="IVK93_000319"
     CDS             complement(5375..6148)
                     /locus_tag="IVK93_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6385640.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6145..6828)
                     /locus_tag="IVK93_000320"
     CDS             complement(6145..6828)
                     /locus_tag="IVK93_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6385641.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6995..7882)
                     /locus_tag="IVK93_000321"
     CDS             complement(6995..7882)
                     /locus_tag="IVK93_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6385642.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7879..8748)
                     /locus_tag="IVK93_000322"
     CDS             complement(7879..8748)
                     /locus_tag="IVK93_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6385643.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8885..10171)
                     /locus_tag="IVK93_000323"
     CDS             complement(8885..10171)
                     /locus_tag="IVK93_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6385644.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10444..11022
                     /locus_tag="IVK93_000324"
     CDS             10444..11022
                     /locus_tag="IVK93_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6385645.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11022..11951
                     /locus_tag="IVK93_000325"
     CDS             11022..11951
                     /locus_tag="IVK93_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6385646.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11948..13684
                     /locus_tag="IVK93_000326"
     CDS             11948..13684
                     /locus_tag="IVK93_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6385647.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13747..14583)
                     /gene="pheA"
                     /locus_tag="IVK93_000327"
     CDS             complement(13747..14583)
                     /gene="pheA"
                     /locus_tag="IVK93_000327"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP6385648.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14580..15089)
                     /locus_tag="IVK93_000328"
     CDS             complement(14580..15089)
                     /locus_tag="IVK93_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP6385649.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15082..16377)
                     /gene="aroA"
                     /locus_tag="IVK93_000329"
     CDS             complement(15082..16377)
                     /gene="aroA"
                     /locus_tag="IVK93_000329"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP6385650.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16390..17481)
                     /locus_tag="IVK93_000330"
     CDS             complement(16390..17481)
                     /locus_tag="IVK93_000330"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP6385651.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17498..18664)
                     /gene="aroC"
                     /locus_tag="IVK93_000331"
     CDS             complement(17498..18664)
                     /gene="aroC"
                     /locus_tag="IVK93_000331"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP6385652.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18666..19730)
                     /gene="aroB"
                     /locus_tag="IVK93_000332"
     CDS             complement(18666..19730)
                     /gene="aroB"
                     /locus_tag="IVK93_000332"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP6385653.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19763..20785)
                     /gene="aroF"
                     /locus_tag="IVK93_000333"
     CDS             complement(19763..20785)
                     /gene="aroF"
                     /locus_tag="IVK93_000333"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP6385654.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21444..22418)
                     /locus_tag="IVK93_000334"
     CDS             complement(21444..22418)
                     /locus_tag="IVK93_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP6385655.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22399..24282)
                     /locus_tag="IVK93_000335"
     CDS             complement(22399..24282)
                     /locus_tag="IVK93_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP6385656.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24300..26525)
                     /locus_tag="IVK93_000336"
     CDS             complement(24300..26525)
                     /locus_tag="IVK93_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP6385657.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26640..27674
                     /locus_tag="IVK93_000337"
     CDS             26640..27674
                     /locus_tag="IVK93_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6385658.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27741..28754)
                     /locus_tag="IVK93_000338"
     CDS             complement(27741..28754)
                     /locus_tag="IVK93_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6385659.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28832..29020)
                     /locus_tag="IVK93_000339"
     CDS             complement(28832..29020)
                     /locus_tag="IVK93_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP6385660.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29460..29588)
                     /locus_tag="IVK93_000340"
     CDS             complement(29460..29588)
                     /locus_tag="IVK93_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6385661.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29591..30964)
                     /gene="rlmD"
                     /locus_tag="IVK93_000341"
     CDS             complement(29591..30964)
                     /gene="rlmD"
                     /locus_tag="IVK93_000341"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6385662.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31075..32130)
                     /locus_tag="IVK93_000342"
     CDS             complement(31075..32130)
                     /locus_tag="IVK93_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6385663.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32140..33570)
                     /gene="gatB"
                     /locus_tag="IVK93_000343"
     CDS             complement(32140..33570)
                     /gene="gatB"
                     /locus_tag="IVK93_000343"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP6385664.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33567..35039)
                     /gene="gatA"
                     /locus_tag="IVK93_000344"
     CDS             complement(33567..35039)
                     /gene="gatA"
                     /locus_tag="IVK93_000344"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP6385665.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35039..35344)
                     /gene="gatC"
                     /locus_tag="IVK93_000345"
     CDS             complement(35039..35344)
                     /gene="gatC"
                     /locus_tag="IVK93_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP6385666.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35450..37486)
                     /gene="ligA"
                     /locus_tag="IVK93_000346"
     CDS             complement(35450..37486)
                     /gene="ligA"
                     /locus_tag="IVK93_000346"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP6385667.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37503..39737)
                     /gene="pcrA"
                     /locus_tag="IVK93_000347"
     CDS             complement(37503..39737)
                     /gene="pcrA"
                     /locus_tag="IVK93_000347"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP6385668.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39892..41451
                     /locus_tag="IVK93_000348"
     CDS             39892..41451
                     /locus_tag="IVK93_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP6385669.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41562..43493)
                     /locus_tag="IVK93_000349"
     CDS             complement(41562..43493)
                     /locus_tag="IVK93_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6385670.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43511..44425)
                     /gene="pfkB"
                     /locus_tag="IVK93_000350"
     CDS             complement(43511..44425)
                     /gene="pfkB"
                     /locus_tag="IVK93_000350"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP6385671.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44422..45174)
                     /locus_tag="IVK93_000351"
     CDS             complement(44422..45174)
                     /locus_tag="IVK93_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6385672.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45333..45542)
                     /locus_tag="IVK93_000352"
     CDS             complement(45333..45542)
                     /locus_tag="IVK93_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385673.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45553..46881)
                     /locus_tag="IVK93_000353"
     CDS             complement(45553..46881)
                     /locus_tag="IVK93_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385674.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47246..47833)
                     /locus_tag="IVK93_000354"
     CDS             complement(47246..47833)
                     /locus_tag="IVK93_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP6385675.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47916..49166)
                     /gene="clpX"
                     /locus_tag="IVK93_000355"
     CDS             complement(47916..49166)
                     /gene="clpX"
                     /locus_tag="IVK93_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP6385676.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49413..50702)
                     /locus_tag="IVK93_000356"
     CDS             complement(49413..50702)
                     /locus_tag="IVK93_000356"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP6385677.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50856..51788)
                     /locus_tag="IVK93_000357"
     CDS             complement(50856..51788)
                     /locus_tag="IVK93_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385678.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51945..52604)
                     /locus_tag="IVK93_000358"
     CDS             complement(51945..52604)
                     /locus_tag="IVK93_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP6385679.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52607..53632)
                     /gene="sppA"
                     /locus_tag="IVK93_000359"
     CDS             complement(52607..53632)
                     /gene="sppA"
                     /locus_tag="IVK93_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP6385680.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53645..53935)
                     /locus_tag="IVK93_000360"
     CDS             complement(53645..53935)
                     /locus_tag="IVK93_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385681.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54344..56149
                     /gene="glmS"
                     /locus_tag="IVK93_000361"
     CDS             54344..56149
                     /gene="glmS"
                     /locus_tag="IVK93_000361"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP6385682.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56221..57576)
                     /locus_tag="IVK93_000362"
     CDS             complement(56221..57576)
                     /locus_tag="IVK93_000362"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP6385683.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57600..58769)
                     /locus_tag="IVK93_000363"
     CDS             complement(57600..58769)
                     /locus_tag="IVK93_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385684.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(58766..59653)
                     /locus_tag="IVK93_000364"
     CDS             complement(58766..59653)
                     /locus_tag="IVK93_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP6385685.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(59842..63534)
                     /gene="nifJ"
                     /locus_tag="IVK93_000365"
     CDS             complement(59842..63534)
                     /gene="nifJ"
                     /locus_tag="IVK93_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP6385686.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            63733..64254
                     /locus_tag="IVK93_000366"
     CDS             63733..64254
                     /locus_tag="IVK93_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6385687.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        17854 a        13935 c        11478 g        21003 t
ORIGIN      
        1 ctcaaccaaa cgtaaaatat gaggaactat ttttgtttgg ctgttcttat tacttgatgc
       61 agggcagctt tttcacaacc tcttattcac ggtgtttcag aagtagcttc tcggaaataa
      121 gccgaaattt cacaaaaatt tgagaaacta tttttaggaa acttcttctt atttcttaaa
      181 gctgaccact tctatcacaa cctctttttg actatgcatt cacatcgatc actttagaat
      241 catcattgat gacatcgcga tctaaatctt tgatgcgtgc acttgtcacg ataccagcta
      301 ccatgctacc gctgacgttt actgctgtac gtgccatatc gattaatggt tcaacagaga
      361 taactaaccc aacgattgct actggcaagt tcattgcccc taaaacgatc agtgaagcaa
      421 atgttgcgcc cccaccgact ccagctacac caaaagaact gattgttaca attgccacaa
      481 gcatcaaaat aaattccaaa ctaaagacat tcactccagc tgctggtgcg acgatcgttg
      541 ccaacatagc tggataaacg ccagcacagc cgttttgtcc aatagacaaa ccaaaacttg
      601 ctgagaaatt agctgttgca tcatctactc ctaaagcttt tgtctgagtc tcgatattca
      661 atggcaaagc tccagcactt gaacgtgaag taaacgcaaa gctcaatacc ggaaaggctt
      721 ttttgaaata gttgattgga ttgacttttg caccaatcaa aatcagtgag tggacaatca
      781 aaacagcaag caatgctacg taagaagcaa cgacaaattt acctaaattg acgatagctg
      841 caaaatcact tgttgccaaa acgtttgtca tcaaagcaaa tactccataa ggtgtcaaac
      901 gcaaaacaag cgtcacgatc cgcataatga ttttatacag actgtcgact aaatctgaaa
      961 agaatgctgc ttcttttggc gcttttcttt tcacgcctag ataagcgatt ccgacaaatg
     1021 ccgcgaaaat aacgactcca atcgtacttg ttgcacgact acctgctaaa tcagcaaaaa
     1081 cgtttttagg gataaaggat aaaatctgct gtggaatcga taagttttca actgtttcct
     1141 gacgtgttgc cagttcagag attcgtgctg tttctgcagc tccttgtgtg aatttagctc
     1201 cttggagtcc gaacagcatt gttgaaccaa ttccgatcaa tgctgcaata gctgttgttc
     1261 caagtaaaga agtcaataca gtcgcactaa ttttccctag cttttcagaa actttcattt
     1321 ttgtgaatgc accaatgatc gaaataaaca ctaaaggcat aactagcatt tgcaagaatg
     1381 atacatatcc ttgacctaca atcccgatcc attccattga ttgagtaacg actttgtcgt
     1441 ctacaccaaa gatcaattga agcagaccgc caaagacgat accaactcct aatgctgtaa
     1501 agacgcgtgt tgaaaatttt gtatggcgct tttgcatacg atagaacaca tataataatg
     1561 ccgcaaaaac tagaaccact aaaactgtga tgagtgttgt catttttctc ctcctcatat
     1621 tattaatgac aagtccatcg aaggaagtaa aggttcccga gaatgattct tcaaccatcc
     1681 tcctgaaaaa atttttttca gtgactttta tattttagac gatttttatc gaaatgacat
     1741 tctttttgct tacaattagt aataaaaaaa catacctgtg atagctttaa tctatcgcag
     1801 gtatgttttt actgtctatt cttgtttttc tgattgaaaa ttcaatttta ccccaggctg
     1861 atctaaggcg atctctgtca cctcataaga cattcggcgt acgatatcga ataacggtgc
     1921 tactagctcg tccacttctt cttgtgtgac atcattttga ccttcaattt tccgattgag
     1981 gatatgattg atctgactca cacttccaga taaaacatat gtatcaaaca ctaaacggaa
     2041 ctctaagcgt gctcctacga tgctatgttc tttcggatag ttttcgatcg tttcttttaa
     2101 aggcgagaag cctactttca aatctgtttc tacttcttgg ttcggcaatc tcatatcata
     2161 atgaaatgct tctacaactt ctttttgtct tctgatttcc atataatttt atcactcctt
     2221 tatgaaaatt ataacatatc tcctttgaaa ggggatacta ctttttaatg atgctctgca
     2281 aggtaaggat ttcattcaga tttcctacca cataatctgc cgggatctca cccagaaaat
     2341 gatcgatgtc gtaaagacaa gtagcgactc ctgcattttt ccctgcttcg atatctaaac
     2401 gacgatcgcc gatcatcatc gtgtctgttc tttctaaatg aaaagtatcg atcaaataat
     2461 ttaaagatgc tggatcaggt ttacgaggaa agtcctgatc gatccctact acttcctcaa
     2521 tcagatgtgc caatcgatgt tcttttaaca ggccccatgt tgattctgtc aaacggtggg
     2581 tcaggatgaa atgacgaccg ccgttgtctt ttagcatttc cagtacttgt ttcgtttctg
     2641 gaaatggacg ggaaacttcc gtactttctt tttcatatcg gtgaaataat tcattaaact
     2701 ctctggcatt caagtttgat tcattgatca aataactagt agaatacttt ttcattttga
     2761 agtagatttc ttttttgtcc atactgatac caaaatcttt tagtgcttgc catgcaccat
     2821 ctaccattgc gggatacgta tcaaacaatg taccatcaaa gtcccatata taatttttca
     2881 tgttcctttt gttccattcc tttgcttctt ttttctatct ctcactttta gataaaggtg
     2941 ttctttaaag tatttttttg tccagaattg tcaaatcatc tgctaatgta tactggattg
     3001 gttgtgcatt tgggatatcg atcgtatcca tttgatcttc aggaacatct tctaaatatt
     3061 ttgtcagtgc tcggatgcta ttaccatgac ccacaactag taaatcttgc cgatctttta
     3121 gttttggtgc tagatgatct tgccataagg gaatgatccg attcactgtc atttccagac
     3181 tttctccgta tgggatcaaa tgcggatcca agtgatcata tctgcggtca aatctatttt
     3241 ctgcggttaa cggcggaact tccgtatatc ctctgcgcca tgtttttact tgatcttttc
     3301 cgtatatttc tttcatctta tctttgtttt tacccacaag atccccataa tgccgttcat
     3361 ttagacgcca tgtttttatt ataggaagcc ataactgacc catttcttct agtgcgactt
     3421 gtccggtaag aatcgcacgt ttcaaaacag aggtataaac ggcatcgaat tgaagtccgg
     3481 cttcaagcat tttttgaccg gcaactgctg cttgttcttt tcctttttct gttaaggcga
     3541 catctagcca tcctgtccag taattttcaa aatttgcttc gctttcgcca tgacgtaata
     3601 atactaattt cattcttttc tcccttaaac attcgtttcg aattttatac cacatgtttt
     3661 gcatgtcaca cgtattcgtc cttttccttt aggtgcacgc tgcttttgat gacaattcgg
     3721 gcactcaaaa aaagtataac tttttcgatc cattttgact tgctttttgt tttttatctg
     3781 atgatatttt tctttgactc tatcaagata tttcaaataa cgttgattct catttagtct
     3841 ggggtagatt ttttttgata ggaaacgaaa tatcatccat ccgaaaaaga cgatgaacaa
     3901 caattgtccg attctatagg gcaaccaacg acttagcaaa ctaaagacaa gcgcagcgat
     3961 caatagaccg cgattcaact gatcaaattt cgcatatctt ccctgcataa gtttgtgata
     4021 gttctgctga aatcgtatta gcctttctaa ccatgtttgc atcatattat ctcctccatc
     4081 atctaatatt tattatgaca aaaaaatcca aaaagaaaaa gccttaattg gccttttcca
     4141 ttgcaacttg gtgaaggaca ttccgatttc tggtaaagtc aatcacaatc actttctttt
     4201 tatcttctct gttgactact cgaaatgtgt ccgtcaatgg aagaaacagc ttcttagatg
     4261 gtagctgttg catacgtata aaacgatgga tcatcgtaat caattgagga aattgaatag
     4321 acgtgtgaag cagacctgga tattttttca acagctgtgt cgttttgatc agattccgtt
     4381 ttttactgat cacttttaaa aagcttttta gaatcttttg ctgacgtcta aaaatagaaa
     4441 aagaattttc gtcatctatt tgataacgca tttgtttttg cagttctgct gcagtcagat
     4501 ggatcttccc atcgtttgta tcgatcatta aaccttctgg aaacagttca accactgctc
     4561 gatcccaaga ttcaagagaa agctcgatat aatcttttac tggtaattcc aattctacct
     4621 taaaaaagtg caaaacagcc gacagccctt ctgcttgata gatttctcct aatgttagcc
     4681 cctctagatg gaactctggt gaaaaatgca gcaatgtttt attttcctgc aattgaacat
     4741 atagaaatgt agatcgctca atcagagtat cttttaaaac aactaaaaaa ttcatttttc
     4801 tccccctttt ttcatcattt ttaaaatagc atattttgaa aagaaagtct gatgcttaga
     4861 gtagaatttc ataaaaattt actatttcat aagtattcac gagaaaaata caaacaaaaa
     4921 agcggaaata acgctcatcc gccttcttgt ttgtgtttat ttcctcttca aacgcttgtc
     4981 tatgattcag acttttctct atcgttattt tcatctaaaa gttcttgcat tccgataatt
     5041 tctcgatatt gatgaatctt agtgatacca atgactttgt ctacatcaat actaaaacaa
     5101 ataccattac ctggaatatc gatgttgatg ccttgaccaa tcgcatccat tactttattc
     5161 gtcaaactat cgggtactaa tgtcaacaca atatcttttt ctggcgaaat gtccaaacct
     5221 aaaaatttcc ctgaatcatg tacaccagaa cctcttccat gtaagatcgt tccgcctgct
     5281 gcacctgctt cttttgaaaa gtcgataaca tcttcgccca ttcctccgtc aacgatcgtc
     5341 acaatcatgt gaagattcaa tgctacaaga tctttcattt gctttccccc aatttttgca
     5401 atctttcttt ttcgattctg tctttttcat cttttcgttt ataaatatat cctagtacta
     5461 aagtggataa tattggtgtc aatgcaatca ttgcaaccac accaaacccg tcaatgatcg
     5521 gatctcttcc ttcaatttgt gcagcgattg ccacgctcat tgataaaata aatgtcgaac
     5581 acatcgggcc cgttgctact ccaccgttat caaaagccat tgctaaaaag atcttgtcca
     5641 cttttcgtcc taaaataaat gctagcccgt atcctgggat caagaaataa taaagagaga
     5701 atccgacgag caaacgccac atagacaatc ctactgctgc gccgatccca attgaaatta
     5761 ccatcagcat gattttcgat cggattctcc cctcgctcaa ttcttcaacc tgtttgatca
     5821 ttacatgaat cgccggctca gcaaacccta ctaagaaccc cattaaaaag ccaaggggaa
     5881 ccagtatata attgtaaggc aatcccgcaa tagcactgcc taaatgctgt cctaccggta
     5941 cataagccat attgactccg tgcaggaaaa ggatcaaccc aatagtcgtt atcaagaaac
     6001 ctttcataat gtcaaagaaa cgctttttgc gcatacgaaa actaatgata ttcattaaaa
     6061 caaataggat tacgataggt gccactgcca aaatgacttc catggtaacg atttcgattc
     6121 catctatcac ttgctgccag aaactcattg gaagatcacc cccaaaatca tcacggcaag
     6181 aatcggccct aacgaagcaa taccgaccat tccaaaactg tcattttcat ttttcttttg
     6241 tttgatcata ctagtcatcc ctccggcaag ggccaagata aatggaacag ttactggacc
     6301 agtagtcact ccccccgaat caaaagcaat cggcataaat tcggaactgg tgaaaaatga
     6361 ggcgatcaaa acgcctatgt agcctatagc catcaattga taataggata gcttgaatac
     6421 gacccgaagc agcgcaaagg ctagaaatac tccagtgccg acgctgacga tcccaatcag
     6481 caacactctg ccaatctctc cgtcagatat atcatatact tgttgtccca aaacttgcac
     6541 ggaaggttca gcaatcgtga tcacgatccc gatagcaaat cccaaactga ccaaaacggt
     6601 caaacttttc tttttgatca tatatttccc tactagatct ccgacttcca tcatagaata
     6661 atctgctcca aacaaaaaga gcgtcattcc gatcatcatg attgccgcac caaccagaaa
     6721 tgaagcgagg tcttctcctt ctaacggtgc aaaaacaaac gttaaaatga caatcaggac
     6781 agtcatcggc aatatagcag caatcacttc tttaaaattt tttagcatgt atcctcctcc
     6841 tttactcttt aagtttacct taaaatgaaa gagttttcga gcacaaactt aaaacgttat
     6901 catttaattt ttctcaacat aaaataacac acgtcccaaa agtatctttt tacttggaaa
     6961 aatgactttg aaggaacgcg cttattgtgt ttttttattt tacagagccg atcattcctt
     7021 gaacgaattg cttttgcaaa acaaaaaata cgatggcagt tggtaaagta gcgatgacta
     7081 atccagccat gatcattcca tagtctggtg tataggaagc acccatagca gataaaacaa
     7141 gcggaaccgt ccgtttatca ggagattgca aagctactaa gggccataaa taattgttcc
     7201 agctactcat gaatgtgatg attgcagccg cggcataggt atttttagcc gtcggcatat
     7261 acactctaaa aaaaatactt agttctttta gaccgtctaa tcgagcggct tctactagat
     7321 cttttggaaa agatttaaca ttttgtcgaa agaagaagat caaaaaagca gtgctaacag
     7381 atggcaggat gacaaccaag aaactgttga ttcccaaaac cgttccgttc aattgtgaaa
     7441 acattcgata taaagggatc atcaatgccg caaaaggaac catcatcgat agcagtagaa
     7501 tattgaaaat taggtctttt cgtttacttt gataaatctc aaatccatat cctgccattg
     7561 acgaaatcag caatgcaaac acagtggtca ccaaagcgat catcgctgaa ttccaaaggg
     7621 aacgagtaaa attcagctca ttggaaaaca aattttgaaa attgatgatc aagttactgc
     7681 caatctttat ttttcccgat gtgatatcca cgggtgtatt cgtcattccc aaaaccatcc
     7741 agacaaaagg gaaaatggaa ataaatgata ggatgcttaa tgctgtatac tttaatactg
     7801 acccaattgt cgaacgaagt tctaatgctt gttttttgac gttggggcgt tcgattgttt
     7861 ctttttctac catatttttc atcgactttc tcctcctact ttcgtttgga ttatcgacaa
     7921 tatcacaata aaggaaacaa taacaaagga aactgctgcc gcatatccaa aattaggcgt
     7981 gaatttataa ctcaaattat agatgtattg agatagggtg gtcgttgcat ttgctggtcc
     8041 cccgccagta atattttgta cttcatcaaa aagctgcaat gtcccgatcg tcgaagtaat
     8101 ggaagtaaac aaaatgattg gttttaaatt tgggatcgtc actttgataa actgctgcca
     8161 tttagaagca ccgtcaatag atgctgcttc ataaatctcc gaatcaatat tttgcatgcc
     8221 tgaaaggaaa aaaatcatat tgtatccagt ccaacgccaa gtgatcgaaa gaatgatcag
     8281 aactctcgcc caaattggat gagtcaacca gccaatcggt gtgtcaatca taccaatgcc
     8341 caacaacaag ttgtttacaa ggccagattg agaaaataga cttttcatga tcagagaata
     8401 actgaccatc gaagtgatac atggaagaaa gatagcggtt cgaaacaacc ctttatattt
     8461 caacttttta tcatttaaaa ggttggaaat aaaaagtgct agaaacaaca tgatcggtac
     8521 ttgtacaagt aaatacagga atgtattaaa aaaagctttt ttaaaagttg catctgtcaa
     8581 catccgctga taattaccta acccgttaaa tgtcatattg acagggggac cactttgaaa
     8641 agacgtgatc aaagccaaaa ccatcggaat aaaaaccatg attgatatca aaatgactgg
     8701 caagattaaa aaagcatgcc cgtttttgaa taaatctttc tgcttcattc ttttccctcc
     8761 aattctatca ttgattatct acatgaaaag acaagaaaga caatatctgt aaggcagacg
     8821 tgatcttttt tgtcttttcg tttgtctgat gaaaaagcga agaaattcac ttttttacca
     8881 ccgatcaatt agccaattgt gcttctactt gtttttgtgt atctgctagt accttgtctg
     8941 tctcttctcc attgatgatt cgatgaagag cttcagctac gactgcttca attgcatagg
     9001 tctcatgtcc gtaattcact tctggtattt cagatgtcca tgttgaaaaa tcgtcaaaga
     9061 tcttttgacc actgtaaaat tcagaagctt cttgataagc ttcttgttca ttcgcgctaa
     9121 gcatcgtaga tactagtccg atttctttcg caagagtgcc catcagttct tcattcgaag
     9181 caaacgttgc ttctaggaaa tctttcgctg aatcttctcc agctactcct ttgatcacat
     9241 accagccagc gccgccaagg ttagaagctt gcgctttttg cagattttct ggaagtgcag
     9301 gtattggtgc aatcttccat tttccagatt ggttttctgc tccttggatc gttgaagaat
     9361 accaagcgcc agttgggcta gaagctactg ctcctgattg tatcgcagtt actccagcat
     9421 tccagtcaga ggtttgttcg actagatctt ctttcaaaag agtggcaaac aattcaagac
     9481 catactttaa agatgcattg tcttggatcg tgactgtctc cccatcttca gatgtatacc
     9541 attctcctgc ctgttgcata atcatcctta ctcgaccaag gtcagaagga ttgacttcag
     9601 cgattttctt tcccgtcttt tctttgacat ctcgcgctac ttgaatatac tcgtcccact
     9661 tcagattctc catatcctct tctgtataac ctgcttctgc catcagatcc gtacgataga
     9721 agtttgctgt tacgcccgaa tcgaaaggaa caccgtatat tttatctcct actttgttta
     9781 ccgcaaattt atacggtgca aaatgatctt cctttacaat agcagacaaa ttagcgaatg
     9841 catctggata agaagtcaaa tagccttgta tcctataatc ttcaatcaag acgatattgg
     9901 gcaatccttc tgtattcccg ctcgctaaag cggtgttcag tttctgaacg atatcatcct
     9961 gagacatcgt aacaacttct atctctgttt cttcgttctc ataaactttt tttgcttcat
    10021 tcactgcttt gatattgaat gtctcatccc acgcccagac agtgactttt tcttctttgt
    10081 ctgctgtact actagcactt ccgcatcctg ttaagaaacc tgatgctgcc gctaaacaaa
    10141 cgactccagc aattaacttt ctgatgttca tttttatccg ctccttttcc atccattagt
    10201 tgtttacgtt tgctacattc atcataccaa aatttgaaaa cggtttcttt attattcaga
    10261 tacttcaaaa atttctccta aaataataaa aaaatacaaa tcaactatga caaaaggaat
    10321 aatcttagca tctagaccaa agatcgaaat ggtgaggttt ctttaaaata aaaatatcct
    10381 tgcattttct cctagaaata agtattttta aattacaaga ctattcttaa ggcgggtaaa
    10441 aaaatgccaa tacgcgtaat tcttatcgat gatgaacaga cgattttaga tggaatgaaa
    10501 cagttgatca attggtctga gcacgggttt caattaatag ggactttttc cagtccgctg
    10561 aaagcgcttg atttttcttt ggtacatgca atcgatatcg tggtcacaga tgtaatgatg
    10621 cctgaattaa atggaatcga attatcgcgg ctattaaaac agcaacaacc tgaaacacag
    10681 atccttatcc tctctagcta tgatgaattt gatatggtta aagattcttt taaagaaggc
    10741 gtcgctgatt atcttttaaa gcctcgattg agtcctgatt tttttctaaa ttccttaact
    10801 gccgtttcac aaaaaatcaa aaaaaagccc aacaaaagcc caatcaccaa tcgttatgag
    10861 cagatctgcg agaatctttc acagcagatt gctggacaaa ctcgtccatt gcttgaccct
    10921 cttctttttg aacacacctt gtttttctgt tatataccga aaaacggcca ttcagttcaa
    10981 aacaaggctc taaacaaaaa cagtcgatca cccattcatt gatggatagc gacgattcgt
    11041 ttactattta tcctgtttct accttttcgg aagaatccgg ctatgtaatc aatactgatt
    11101 ccaaagatac acttaggaag tttgttcaaa agtttcgtca agaagttttt ccaggtgatt
    11161 tttttgttgt ttctgattct tttgcaatcc aagatatcaa tgaaatattc caagagatca
    11221 gaaattgcac caaaggacaa cgtttctatc ataagggaaa gaaccttgct tatcagcatg
    11281 aacttcttcc gttattaacc aaccacgagc agcaagcaag tagttattta acaaaaatcg
    11341 ttcataaaga ctacgcttta gctgtcaaag aacttgagaa tttcttcacg acgatattgg
    11401 aacagcctat tgatccagct tatttgaaac atgaggcaat caatcgtttc tatgcattac
    11461 tcaacgcttt ggctgacgag catgttggga atgaagaact ttctttgctc aagatcgccc
    11521 ttccttcttt attagccgat gctgagtatt tcgaagattt ttctattctt gtcaaagaaa
    11581 cacttgagaa actcacccac ttttctgcaa atgcaaaaaa agacaatttc gatatcttga
    11641 ctttgatcta cgagttcgtc caggaaaatt atgagaaaga aatcaattta caacttttat
    11701 ccgaaaaata tcatttttca tatagctatc tctcagccat ctttactgaa aaatttggta
    11761 tcaacttttc aaaatatcta aaaaaagtcc gtatcagtaa agccaagctc tttttgacgg
    11821 aaacacggct tactttgacg gaaatctgtg agaaagtcgg ctataccgaa ctgggttatt
    11881 tctccagagt ttttaaagaa gagacaggaa tgacaccttc tcaataccgt aaaggaactt
    11941 ttgtaaaatg agaaaaaaaa tcaaagagca tgaactcttt gccaaacttc tgttcatttt
    12001 gttcatcggt ctttttctgc aagccatcgt catttctttg tttatttatc atcgatcaag
    12061 ggatgcctat atccagctgt tcaatcaatc gaatgatgtg gtactaaaaa agattcaatc
    12121 tgaatttgaa agtttgaatg atacaatcga gaatacatta gccgcttttg attctaatcc
    12181 agctgtcaaa agctattttt ccaatgatcc agcccaacat atggaacagc ttcagcaact
    12241 aagaacaatc caaaaaatga atgactctct ttcaaaaatc catccaatga tcgattacga
    12301 tgtattgatc tttggagaaa atggaagaac ttttgtcgga aacgacatgc tcactgccgt
    12361 atctgccgat tctttttttc aatctgcgat tgctcagaga gtgaatgaac gtgctgctga
    12421 tacacaaatg ctttttgcac accacggatt gactcttcgc gataagaaag cacccagcgt
    12481 cttctttgtc aaaaaactga aaaatacatt gaatcacgtt tacggatatg ctgttttatc
    12541 gatctcatcc aagcaactag cgaatctttt tcaatctgta gtaaatcctg agatatcgaa
    12601 gatctccttc atgaacgaag aacagacgat catcgcatca aatgaagaaa atacaattgg
    12661 gagccgatca atgattttca aaaaactaac agtaggtgaa acaacgattt caaatggaca
    12721 gcagttgacc cgtcttcctc tgtaccgcca ggattgtaca ttactcagtc aagtcgatct
    12781 tcattcttta tctaaccaaa tgggcgtgat ttttccgatt attttgtata atatcgtgac
    12841 acttgctttt gctggctcat tggtatttat gtatttggat cgacacacaa aatcgatcta
    12901 ccgcttgatt gattcgctaa aacggctaga ggaagatggc ttaaaagcta ctgttcctgt
    12961 acaaggaagc catgaagtcc aattgctagg gaatacgatc aatcaattgc taagtaatgc
    13021 tcagcatcat tatgaacaga cgatccaaga cgaaaagaag aaacgttcgc ttgaaatcca
    13081 aactatgcaa gcacagatac agccccattt tatttataat acgctgacta gcttgaaatt
    13141 tttgatttgg cagaaaaaaa cagatgaagc agttcaagga atcgacagtt tcattacttt
    13201 actaagaagt acgattggga aaaaagaaga agtgattcca gtcaaggaag aaatcaaaag
    13261 cgtacaaagt tacatcgatc ttttgagtat gcgatatggc gaaagaatct cggctaaaat
    13321 aatgattcct gatgaactgc agtcattatc tatgccgaat atgatccttc aaccaattat
    13381 cgaaaacgcc tatcttcatg cttttcacaa taaaacgcaa ggattcatca cggtgtacgg
    13441 caaaacaaaa aatcagcttc ttttattcga agtgattgat aacggcgatg gcttcgatac
    13501 tgaaaatacc aaacaaaaaa atgatcattt ttcaggaatc ggcatcagcc atgtagatga
    13561 gcggatacgc ctaatgtatg gcgatgcata tggcttaacg atccaatcag tgattgggac
    13621 aggaacgact gtaacgattt gtctaccgct tcttcctaaa aatcaatcag aatatcaaaa
    13681 ataaagaata gccaagcgct gattttttta ttttaagtta aataaatcaa tcagtattct
    13741 tgactttcat ctacgcgtct cttctactgt cagcactgga taacagccca atatttgact
    13801 ttctcctccg agcatcttga tttcttggag cgcattctct actaatgtca ttggacggtc
    13861 caacaacaaa tcgatcacga aaaaatattc tcccaagcta gttttcaatg gtcgggattc
    13921 aatcttactt aagttgattt cccgccagcc aaaagctgcc agcattttat gaagcatccc
    13981 tggccgatta gcaggtagtg tcaagatgac actcattttt actggagctg actgctggct
    14041 ggtcatcttt cggtcaccga cgatccaaaa acgtgtctgg ttcaactcat tatcttggat
    14101 attctcagct aagatctcca atccaacatg ctgtgctgtt tctaatgaag caatcgctgc
    14161 tgcgtcttct ttcggatgtt ctgctacata cattgctgca gcagttgttg attctgttgc
    14221 tactaaagga acattaggat aatgcgtttc taaaaactgt tgtgattgtg ctagtgcttg
    14281 tggatgtgaa aggatttttg taatctttgt tgctggattg cctaaaagct gctgcttgat
    14341 aggaagaacg atttcacttt taacttcgac ttctggatgt ttggaaagaa gatcgattgt
    14401 gtgatgaaca gctccttcca atgaattctc tacaggaacg acagccagat cgacttgttt
    14461 gcgaaacaaa gcgcgcaaac aaacagggat ggaagcatac gcagtcaatt gttctgtagg
    14521 aaatagctga caagccgcct gataagtgaa tgaactttct ggccctagat agctgacgtt
    14581 catatgcttt tgatcctttc tataatggta tcgatgatct tttctggtgt ttgattggtc
    14641 gtgttgattc gatgtgtcgc actttcttca taccaagaaa gtcgttgtgc caataatgct
    14701 tttatttctt ctcttgtttt tccaagagct aaaggtcgaa tagacgtttc atcttgttgg
    14761 atacgatcaa ctagacagtc cgcttctgcc tctaagtaaa gaacaatcgg ctgtgttttt
    14821 aaaaaacgcc gattttctgc actttggaca atccctccac cagtcgcgat aattttgtct
    14881 tcatttgaca gctttcttaa aatatctgac tccacttttc gaaagctttt ttcaccgtaa
    14941 tagtgaaaat attcggcaat cgacaattgg atatgttcct caatctttgt atctaaatct
    15001 acatactctt tttgtaaaca ttgagctaac cctttaccga ttgttgtttt tccagcgccc
    15061 atgaaaccaa tcagcaaaat gctagccatg agcttctcct cctgacacaa gttctgctaa
    15121 atcgtcaaaa aatgccggat aagagattga aacagcttca gctccttcta gttcgcaagg
    15181 tccatcagct atcaaagcag ctacttgcag catcataccg attcgatgat cgcctctgct
    15241 ggacactttt gcgccatgca gaggtgtcgg cccatggatg atcagaccgt ctgctgtcgc
    15301 ttttatcttt gctcccattt tctgtaattc ttctgccacc gcatcaattc tgttggtttc
    15361 ttttactttt aattcttctg cgtctttgat gatcgtgatt ccttctgctt gagtagccac
    15421 taaagctaga atgggcaatt catcaatcag acgcggaatg atttctccac caatcgttgc
    15481 tttcttcaaa tgactcgtct tgacacttaa atcagccgat tggttctgtt cattgtgatt
    15541 cgtctgggta atcgctgcgc ccatctcctc caacacatct aagataccag ttcttgttgg
    15601 attgaccccg acatttttta acaacagttg gctttccgga acaagaagtc ctgctgctag
    15661 aaagaaagca gctgatgaaa tatcacctgg aacagttatc tgttgaccag ttaatttttg
    15721 cggtccggtc actatgatcg ttttatcttc cactgtgatc cttccgccaa attgacgaat
    15781 catttcttcc gtatggttac gggaacgttc tttttcaacg attacggatg tgccttctgc
    15841 ttgtaatgcc gcaaacagca aagcagattt gacttgcgca ctggctacag gcatattgta
    15901 tcggataggt gataaagagc gggttcctgt cacagtgatt ggtagatact ggtcattttc
    15961 ttctccttgc aagtcagcgc ccatctcgcg taaaggttcc atcactcgcc ccattggacg
    16021 tttagacaac gaagcatccc cgaacagagt agtcgtaaaa ggctgtccag ccaaaatacc
    16081 catcaataaa cgtatggtcg taccggagtt tcccatgtca attgattcgg ttggttggac
    16141 aaatgagtgg ctaccgcgac catgaatctt gatcacctct tcctcttctt cgatctcgac
    16201 acctaattgt tggaaggcat gtaaagtact cagacaatct tctgcgcgca aaaaattttg
    16261 tatagtcgtc gttccttcag caattgctcc aaacatgatg ctgcgatggg aaattgattt
    16321 atctgctggt atcctaacag tccctcttaa tccatgtatt tgttgtaata attgcattta
    16381 tccagcctcc tatttgatcc agcaatgata actggtatta gacataatga ttctttttgc
    16441 ttgtaacaaa tcctgttggt ttttaaaact gatttgcagt attcctaaaa tatcttccct
    16501 ggtctcttgg atcttcaaat taatcaaaga aactcccgct tttccgatca gtccagttac
    16561 ttctgcaata acgccaggtt catctggcac atcgataaat aaatcaaaaa aggcaggaat
    16621 cgcgccttgc ttatggacag gcaactggtc tcgactttct tttgcccgat tgaaaaaaga
    16681 aaagatttcc gattggttct cagtcatgat ccattctgct atttgtgtca tttcttgttg
    16741 ccaatctgcc aatcgttcca ataatatctg cttattgctc aacaaaatat ctgtccacat
    16801 gacaggatct gaggaagcga tcctagtgat atctcgaaag cctcctgctg ccagttgttt
    16861 ggctcgcgga tactcttgat tgaattgttc tgcctgattg actaagccag ctgccagaat
    16921 atgtggaaaa tgacttagca ttcctgttat acggtcatgt tctcttgctg taagagttac
    16981 atattttgct ttggttccgc aaaatagttc ttgcagtttt tcaacatctt ttttcgtctt
    17041 ttccaaaggt gtaaagatgt aataggcatt ttcaaatagt ttagcatctg ctgcctgtat
    17101 acctgattta tgagaaccag ccattggatg tccaccgaca aaccgaagat ttttctgctc
    17161 agcggatgtc acgacttgct gcttcgtact gctgacatcc gtaatgatga cgtgatcaga
    17221 caagttgatt ctttcaagga catcaagata agctaaggtt gtacggacgg gaacggctaa
    17281 taagataacg tctgcttgtt ctgcacctgt ttcaaaatca cttgcaacgt aatcaacgat
    17341 ctgctgttct actgcttgct cacgggtttt tgactgacta tcccaaccaa ctactatcag
    17401 ttctggattg gtctgacgaa tacaaagtgc catagaagca ccaatcagtc caagccccac
    17461 gataaaaaca tgttgtgcca tcttatttgc ccccttttta gtaatgtttc aagtagtcac
    17521 ggtactcctt cactgcagct ttcaactcaa caagcgaatc acttgggaat tttgacaata
    17581 gctctgtcgc taccacagtg gctacaacat tttccgcaac gacgctagct gcaggaactg
    17641 ctgtactatc cgaacgttct acacttgctt tgtaaggttc ttttgtatcg atatcaacac
    17701 tttgaagtgg cttgtacaat gtaggaatgg gtttcatgac cccgcgaatg acaagtggtt
    17761 cgccattggt cattccgcct tcgaagccgc ctagattatt ggtacgtcgg aaatagcctc
    17821 tttcttcttc ccataggatc tcatccatca cttctgagcc tggtaatgaa ccagaagtga
    17881 atccgacccc aaattctacg cctttaaagg cattgatact aaccacagct tgtgcgattt
    17941 tggcatctag cttcgtgtcc cattgtacgt agcttcctaa tccagctgga acgcctccaa
    18001 cgagcacttc tacaattcct ccgatcgtat ctccattttt ctttgtctga tcgatcaaat
    18061 cttttattct ttgctcaact gctggatcaa caactcgtac ttctgacgct tcagaaattt
    18121 ctttgatttc ttgaatcgag atatttccag gtatttctgc ttggattcca ccaagacaaa
    18181 tcacgtgtcc tgctacgatg atgtcaagtt cttccagaat tttcttggca attgcgccga
    18241 ttgctacacg catggtcgtc tctcttgcag aggaccgttc aagcacattc cgaagatctc
    18301 gatgatggta tttcataccg cctaccaaat ccgcgtgtcc tggacgcgga cgtgcaactc
    18361 gccgcagtga ttggtctttt tcaggcactt ctgctacaga catgacagtt gtccaatttt
    18421 tccaatcttt attttctacc actaaagtga caggtgaacc aattgttttg ccgtgacgga
    18481 tgccagacgt gatccgaaca cgatcttttt cgatcttcat ccttcctcct ctgccgtaac
    18541 cagcttgtct gcgagcgagt tcgtgattga tttcttcttc tgacagaggt aatcctgctg
    18601 gtagtccttc aataatggct gttaattctg gtccatggga ttctcccgca gtcatataac
    18661 gcatcttaca gtcctcctaa gtaatctttg aatgtctctg tgggaacagg aacgattttt
    18721 gcttgcccaa tcttttctag caagatgata ttcaattggc ttcctcttgc tttcttatca
    18781 tgggttatcg cgtggaataa ttcatctgga tgacttagat cgtacgtaat cggcagatgg
    18841 aatttttgaa gtatcctttc taaagcagct gtcgatccag ttggagacaa gccgattttt
    18901 tctgcatgag aagtgatggc taccattcca atagctactc cttctccatg actgactacc
    18961 ccatatcctg ctgtattttc aattgcatgg ccaattgtat gaccaaaatt taaaataagt
    19021 cgatttcctt ggtcaaagac atcttcttcc actacttttc gtttgatttc caacgtagca
    19081 gtaatgattg gaacagcatg ttccagaaga tctttttcgt ctttcagctc gttcagctgt
    19141 ttccaaagcg ttgcatctgc tatagctgct gatttgatga tttcagcgat tccttctcgt
    19201 aaacgacgta ccggcaaagt ttttagaacc tcaggatcaa tcaatacacc agctggctgg
    19261 ctaaatgttc cgacaaggtt tttcgctttg tttgtattga ctgctgtctt cccgccaata
    19321 ctagaatcta cttgtgcaag tagcgtagtc ggaatttgaa cgaaatggat cccacgcatg
    19381 aacgtcgagg ccacgaatgc tgctagatca cccaccattc ctccaccaag tgcaagtatc
    19441 ccatcgctgc gggtaaaatc attatctgct aaaaaatcgt ataactcagc tgcttgttct
    19501 aaagatttac tggtctcacc cgccggtacg attgaaagga ctggttcaaa attagcttgt
    19561 ctcaattgga acatgatttt ttctgcatat agtggagcaa cgtttgtatc agtgatcaca
    19621 gctacttttt gaggtctcca aatcgtttga agccatgatc cagcctgttc tagtgcgcct
    19681 ttttcaatat gaatatcgta tttttggtta tcaacatgta cagtaagcat tcgtttcact
    19741 tctttctttt aaaaagctgg aattaatcga gtgtctgaat cttttccatt gcttctttca
    19801 tgatcgcaac ttctttcatc atgcgtgagt aagtcttttc attcaacgat tggggaccgt
    19861 ctgaccaagc atttgcagga tcaggatgga tctctacgat catgccatct gctccgcttg
    19921 ccacacccgc acgagccatt ggtggtacaa gttcccaaat tcctgtacca tgacttggat
    19981 caacaatcac agggaaatga gttaattttt tcatcaatgg gacagcactt aaatcaaacg
    20041 tattacgtgt cgctgtttcg taagtccgaa tcccacgttc gataaaaatg acaggagatt
    20101 tgtcttcgac tgcaatgtat tctgcagcat taagccattc attgatcgta ccggaaattc
    20161 cacgtttcaa tccaacaggt ttacctgttt taccgacagc agatagcagt ttaaaatttt
    20221 gcatattacg tgcgccaatc tgtaggatat cgctgtactc agccaccata tcgatatgcc
    20281 cttcatccat cacttcagta atcactttca ttccaaattc atcagccgct tgacggatgt
    20341 atttcaatcc ttcttcttct aaaccttgga aagcatatgg agaagtacgt ggtttgaagg
    20401 caccacctcg cagaattttt gcgcccccgg ctttggccat tcgcgcacat tctctgattt
    20461 ggtctaatcc ttcgatagaa caaggaccag ccatcgtcac gaaactgccg tcaccaattt
    20521 ttactccgtc tacatccacg actgtattgc caggatggaa ttctcgagaa gtcaatttgt
    20581 atgtgtttaa aatacgcaca gcattttcta ctccttcata gcgtgtaaat gctccttctt
    20641 gtaaacttcg cgtatcacct tttagtccaa tcactactcg ttctgttcct tgatcgattg
    20701 agactttgaa tccttctttt tcgatatgat cgacgacttt ttttatttgt tcttccgttg
    20761 cttctttttt cataattact atcattgttt ttcccgcttt cttttgttta cttatctatc
    20821 ttggctgatt ttctctactt tttcaaggtg gttttacatt aattcctact aaatgaccgt
    20881 gtcctgttgt tctcattttc gctccttctt tcctacttat gacccagata atcaacaaaa
    20941 aaagatgtcc ctgtaggaaa ttgcctctac agtgaacatc ttcaacgagc cttaaaaagc
    21001 gctgcaaaaa tataaaatag ccactataga tgaatttctc tacgtggctg taagtcgtgt
    21061 ttaaaaatct ttagccatca tagatacaaa cctggtttgt ttgtacccac gaagtcatga
    21121 atacaaacct atctaaaata gctaaagtaa tacgtattca gttttgtaag ttttctagat
    21181 ttcataaaaa atcgctccaa actgatgtaa gaattgtacc tagtatagac ataaaaaaat
    21241 cgactgtcaa tcatttttat gaaacttttt ttaattttct gatagttaac tttgtttttc
    21301 agaaaaataa caattggttt tattgaagaa aatagtcatg aacaaaagta tctgagaact
    21361 tttttactct cttgttcatg acatatagaa taaagcagct ttgtcttgta aactccgttt
    21421 ttcaaataat aatctaaaga ttgctactga ataataaagc cgatttcatg gtcctcttcg
    21481 ctgctgattc ggtaaggtga ctcaacatcc gctccccagc tattgatacc cccgactcct
    21541 cttacttttg caaaaatcgt aagaaccgtt cgtctttctg gcggtagttc ttctatatgt
    21601 gttgcctgtt ccagttcttc tgctttgaat ggtaaagcgg aaaaagcaaa tggcgtatcc
    21661 tgctgttcaa atcgcaacga aaaatcttct tgatctttat ccgtattatc caacgtttga
    21721 tttcttgtta gttctaccca tttcgtttcc atatgcatgc cgcattcttg gggaacaagg
    21781 tatggggaaa cggggagtcc tgccacttta taaatacctt cgacaccacc agccatccga
    21841 tccggatatg tttcccctga taggccttca tactcaaaaa attttgcttt cgttggcata
    21901 atgaacctta aacccaatgc gggtaattcc ggtaatcctt tttgtccata ataatgcata
    21961 ttgacaagca ttttaccctc tccagcgatt tggtaggaaa ttgtcacttg ggtttttggt
    22021 actgtgttga tttccagtgt aaaaatgatt tcaaccgttt cagcgtattc attacttgaa
    22081 tattgattat ttactggagc aatcggcaaa tcgatctttt tattgtccac cagtacttct
    22141 atttctttga cttttggata caatccggct cctaaccaca taccggattt aaatccaaat
    22201 ccgctacctc tgtcattatc ggtcgttgct cgccaaaaaa ctggaagtgt ttcccggtat
    22261 agccattctt ttccgttttt attgagcgat tccagtccgc ctcttgtata agaaaaaata
    22321 taatgcgtat tccccgattt gacacctaat gtacagtcgc cgaagacgat tgcaactttt
    22381 tccgtatcca tatgactttt atttactgta tttttcataa taatatctta cctcctgcat
    22441 ggcgggtttt tcttgtccat ctgcaaaaac gatcccattc cctgagaatt catagtcact
    22501 tggtttatcg tcaaagtcac cgccatagcg catcacttct ctgcctgtga ttggatcagt
    22561 cacttggatc gcctgatctt taaaatccca tatatagcct ccttggtaca tttcataacg
    22621 atcaagcaac gtcatgtaag aatccattcc acctaacgaa ttccccatgt catgcatata
    22681 ttcacataag atgaacggtt tttccgcttt attttcaagg tagtcaatga tctcttctgg
    22741 tggcgcatac atgcagcttt ttacatctga aatcgaatct tcgaatgctg gatttggaaa
    22801 attcccttca taatggacaa gtcgatcggg gtctactttt ttgaagtaat cgttcatttg
    22861 tcgaatgttt tcttccgcat aagattcatt tccaagagac caaaaaagaa tacttggatg
    22921 gttttttaat gtttcgaaat tcgtttttgc tcgatcaaga acagccgcct gccactgagg
    22981 tactgcacct ggtacgttgt aagaaggctc aaatgcgccc attttttgcc atgagccatg
    23041 ggattcgagg ttcgtttccg ccatcacatg gattcctgct tggtcgcata aataatacca
    23101 agtgatctgg ttggggtaat gagatgtccg tacgccgttg atctttgcat tttttatgat
    23161 agccaaatct ttttccatat cttctatagt gatggcgcgt ccttttttcg gatgccactc
    23221 atgtcgattt accccgttga taatcatccg ttttccattg aagtagatga ttttatcttt
    23281 gatttcgatt ttacgaaaac cgaaggaata gtcaatatac tccgttactc ttttttcttc
    23341 gtctatcgtc tccaattcaa gcttatataa attgggctga aaatgccccc acagtgccac
    23401 attttcaaat ccatcgatgc gaatcgtcat ttgctctttt aacggatatt gctgttgata
    23461 gatttccttc ccatcaggat ctagtatcgc tacattcagc tgactgtttt tggctacttg
    23521 ctcaaaagac aaatctactt caagtttgcc attttgacat ttgtcatcta agcgaggttt
    23581 gatccaaaga tcttctaaat ggttttgtcc tcgagcaact aagaagacat ctctgaagat
    23641 cccaaaaaat cggaaaaagt cctgatcttc caagaatgca gctgtacttc ttttgtaaac
    23701 ctccacagct agaatatttt cttcttccaa ataaggtgtt aaatcaaatt cagacggagt
    23761 gaacgaatct tcggcatacc cgataaaatg accattcaac caaataaaca ttgcttgttc
    23821 tacaccagca aaatgaatcg agatttgttt tccctttaga ccttcttcta atcgaaaagt
    23881 cgtcaaatac gaaccaaccg gattgtcttt agcagctgaa aagatccctt ccgcctcttt
    23941 ctcagggctt gtcgtgaaag ggggacggcg aaatacatgt ccctcccacg gatacatcgt
    24001 attgatgtaa tggatcttgt cataatttgc taattcaata tgactaggaa cagtgatttc
    24061 atcaaaagaa caggcatcaa aatctttttt ataaaatgtt tttggtcgta ccgaaggatt
    24121 tggcgaataa caaaaacgcc atgatccatt taaagactgt ttaagggagg atgtggtttc
    24181 tacttcttcc cttgttgcat aacaaacatg atcgctatgt gcatttgatt ggttcactct
    24241 aaaaacttgt ggatcatcta accattttat atttgctttc atactttttt ccttctctct
    24301 cataactttt tgaaatgaag cagttctgtt tggaaatcac cttgaaaatc ggggaagtaa
    24361 agtccaactg tcatcaattc ggcccccgaa aatatccgct tcgaggtctg ttcctgataa
    24421 agtgcctcat catctaaccc catcagatat acttcatgga acgctggttg agctgaagct
    24481 agtatcctcc ctaaaaacac aatagcttca tcttgtttag gagacacaaa catccatgcg
    24541 gcaatatttt cttcaaaagg actcgccaaa cgataataat ggccatactg gatcaccgga
    24601 cgaatcgttt gataggatat aatttggtct tttacttgct tttcttcttc aggagacagc
    24661 tttgtcaaat ccagttcata accaaaaaca gcgctcatag ctgtttctcc tcttgttttc
    24721 aacgaagtag atctgtcggt ctgatgattt ggtacagctg atacatgagc tgtgatggac
    24781 gaaatcggat aagcaaggct tgttccatat tggattttca tacgctccac tgcatccgtg
    24841 ttatcacttg tccatgactg cggcatataa taaataaacc cggcatcaaa ccgtccacct
    24901 ccaccagaac agccttccca taagatttcc ggataatcag ttgtcaaatg ttcaagtagt
    24961 tcataaagcc cgagcatata acgatgcgct acttctcctt gtctctgtgg gtcaagggta
    25021 atcgaataga catctgaaag cgaacggttc atatcccatt tgatgtagtc aagaggaatc
    25081 gtatctaaaa tctttctcat ttgtttctca atcgtctgcc gaacatctaa ccttgtaaaa
    25141 tccagtacat gttgtgatct ggaagcagaa ggcattctac ctggctcctg cataagaaaa
    25201 tcaggatgtg ttcgatacag ttcagaatca accgagatca tttcaggttc aaaccataaa
    25261 ccaaatttca atcctttgct atgagcataa tctgctattt cacgtaatcc atttgtcaac
    25321 ttgccttcat actcgaacca gtctcctaat gaagaattgt ccgcatctct ttttccaaac
    25381 cagccatcat ctaatacgaa catttcgatg cctagctcct ttgcttcatc aatgatcgcc
    25441 ttgatttttt cactattgaa gtcaaagtaa gtggcttccc agttattgac cagaatcggc
    25501 cgttcagcaa attgatgacg acctcttgcg attctttcac gtagcaactc atggtggatc
    25561 tgactcattt tgttcaatcc ctgacttgag taagaaagga tcatttcagg tgtttgaaaa
    25621 gactggcctg cattcaattc ccacgaaaaa cgatggctgt tgatcccacc gacgactctc
    25681 aattgattga tctgatcttt ttcaagttcg aaagaatggt tgccggaata aacaaattga
    25741 agtccgatcg cttctccagt attttctgtc gtatgatgat gaactaaagc aataaagtta
    25801 ttcatatggt gactgctcgt tcctcgacgg ctttcaaata cttttgttcc agataaaaca
    25861 gattgccgtg aaatctgtct ttcattgaca tgtgctcctg gcaaagcaat gacttcatca
    25921 aatcgcctag tatgggcaaa gtctagctgg aaactagccg ctttttctag tttgattgtt
    25981 tctccggttt catttcttat ctttacagat cgtgtgatca ctgctctatc agtaaatatg
    26041 gtatagaaca aatcgaaata aatcgctgtt tctctatcct taagtgaaat caccaacgtt
    26101 tgtgcctctg acttgtccat cacataagtt tgaggcaagc cttcgatctc tggcttccca
    26161 tctgtaatat aatggctgtc atatctcaga tccagtaagt ctgatccatt cttgcgctga
    26221 atgatcgatg cgggcgttcg aaagtccatg ctgccatgag aagagtattc ttgcggtagt
    26281 gtatctttcg acagagaccg gtcaggattt aagggaacat ttcctgaaaa tccacgatct
    26341 cttcggggat aatttttatt gtctttgtac tgcttgactg gtttgccaaa ataaatatgc
    26401 gccaatacat tgctttcttc tatctttatc agataagaaa gaaatgtatt tcgtaaatgg
    26461 aagactcggc tcttttcatc aaataagatg aaatttttat ctgtttttct atgcgtaata
    26521 tccatcattt cactcccaac aaaacggaaa ccgtttactt ttttttataa ttcctgtata
    26581 ctgtttacta aacaattttg tcaataaaaa ttttttattt ttttgaggag tgtgtaaaaa
    26641 tggttggaat tagagatgtc gcacgaaagg caaatgtctc tcctgctaca gtttcccgtg
    26701 tgttgaataa cgatcagacg atccatgtgg ctcagactac tcggcaacgg atagtagagg
    26761 cagctgctgc gttggattat gacatcagta ctcgtaaata tatcaaaaag agaatgcctt
    26821 cgattggtgt gatctctacg atcggcaaag cgagcgaaga acaggatatc tattacaaac
    26881 atttgcgagt agggatggaa gaagaagctc gacgtctgca tttagggatg aatcgaatct
    26941 ataatctatc tgataatcct aaacaatggg aagatctaga tcagttaggt gcggtgatcg
    27001 ttgttgggac tgttacgaaa gaatcgatta ccgccttgtt aaaacaaaac atgcaaatag
    27061 tcgtcgtgga taaccctgac atccagcaag aggtcgatat ggtgtatgca gatcttgaac
    27121 ggatgactaa aaccgtactg gaattgttct tagaaaatgg tcataaaaga atagcctata
    27181 tcgggggata taaagtcgat atggatgaat tcggtgggaa gcagatttct ataaatgaaa
    27241 agaggatgcg tgcctatcag cattttatga tcgatcatca cttagaaaaa tatatgtctt
    27301 atcatctagg tggctggaca gaaaaacaag gtgaggtact tgcgactaat ctattagaaa
    27361 atgaatcccc tcttcctact gccattcttg tcgggagcga tccaatggca attggtgtct
    27421 atcgggcatt gaaacaaaaa aatatccaaa ttggagaaga gattgttgta gcaagctttg
    27481 ataatatcga gacaatcgca aacctctccc ctggcttgac cagtgttcaa atcaatgcga
    27541 aatcaattgg gcaagcagct gttcgaatgg cattggagcg aattgacggt atacgggacg
    27601 aaccaatcat tttgacttat cctgcacgat tgattgttag ggaaagtttt atcccaagaa
    27661 caaaaaggta ttgaaagaga ggttgtgaaa agccgcttcg tttcttcata ctcaaaacga
    27721 aacggctttt tcacaacctc ttacttatac ttattcacaa aatccaatgt ctgctggtaa
    27781 caattttgcg attcttttag cgtgtaatca aattggtatt catgggtaat ctcttttttt
    27841 gtatctgtat aaaacaagga ttgcacgggt acctttaatt ctgtcaaccg attttctaat
    27901 gcttgacctt gctcctggaa tgagtatgca tttccgtcag ttatatacgt tggcggaaat
    27961 tccttagaaa catggtcagc cacactagct tctttcaatt gctcactcgt tttccaatct
    28021 tttgttccaa tcaacgacca cgcaacggtt ttgacgaaaa acttcataaa tcgatcatcc
    28081 gaatgctgct gtgctgttag cttcaaatca acaggtccgc aataagaaat ggttcctttc
    28141 agactatctt ttggaagtag atgtttgatt ttcatttgtt tagcatacgc atcattcgtt
    28201 tgtatagctg catattgcaa tgcgatttgt gaacctgcac tatctccacc aaaaaataat
    28261 tgttccatgt ctagcaattt gtcgtctttt tcttcagcta ataaatcttt gatcagttca
    28321 ttcacttgaa gaacttgatt agggtattct gaatcaggcg ctaattcata attcatcgca
    28381 accactgcga tgttggcgtc tgaaactaat tttgtcgcaa attctttgac acccgattta
    28441 tctccgccga cgaatcctcc accatgtacc caaaatagaa caggaacagg ccccttgctg
    28501 ttttttggat agtagatgtc ataagtattc tctttatact tggactcata cgtcaaatct
    28561 gtttttttcg ccacgttttt ttctgcttga tcgaacgttt ttttatcagt gatttgaaca
    28621 gtatttgaaa acatatggtt gatcaagaat gctccaggtc tgggggaaag ctggaaggca
    28681 agaaagattg caaacgcgat cccaagaatg attccgaaga accatccgat aaatttaaga
    28741 attttctttt tcataaggct ccctcagtag ttagtattaa gcattctgat tataccatag
    28801 tgttatttcc tatcaagctc tcctagatga tttaatgatt ttccccaaac ttcttctgca
    28861 atgcttttga caaaagaaat tttttccatt gttcttcaaa caaaacattt tcttcttctg
    28921 ttgaagcaaa tccacattga ggacttaagc acaaacgatc caaaggcaga tatcgtgtcg
    28981 cttcgtagat ccggtcaatc acttgctggt gctcttccat ttcgccattt ttcgttacag
    29041 tcaaaatcta agacaactaa tttatcttca gagactttct ctaaaagaac aaaactccca
    29101 gcacgttcac tatcgtattc taaataatag gcacggacat tttcttgagt aaaataatgg
    29161 gtctgctacg tcgtcatccc ccctgatgcc gccaaaatcg aataataatt tccgcgtcaa
    29221 acatgtatat ttagtgttga atcatttgag aatatgaaaa taaaaatctt tttgatcaat
    29281 cagttaatca aattgaaata acgaaaaatc tgagacagaa tgacgttcat tctgacccag
    29341 atttgatttt gcacaactca acagtcctta ttggcaaatc gccaaaataa tgtagactaa
    29401 ctaaaaagac taagaaacaa taacaaaagt taacacttct tagtctttct ttagcacgtc
    29461 tatttattaa agattttagg gccttttctc atgtttttac gatcattctt aggtgtttcc
    29521 atttgtttgg atcggcctcc accttgtttc tttttgatca attctttcat tttttctttt
    29581 tggtccattt ttattctcct ttaacgaatg aagcgactaa ttcgatatga tgggtttgcg
    29641 ggaaaagatc tacaggctga actttcttca cggcataacc tgcttctgca aataacttag
    29701 catctcttgc aaaagttgcc ggattacatg agatatagac gattcgttct ggtgacattt
    29761 cgattgctga ttcaataaaa tgatgatcta atccttttcg tggagggtca actacgatca
    29821 cagatggttt gatccctttt tcttgccatt ttttcataac tttttctgct tttccgactt
    29881 cataatgaac attttcgatt ttattattta aagcattgaa ctgtgcatct tcaattgctt
    29941 ctggaataac ttccatccca tacaccttat ccactctgtc tgctaaagac aagccaatcg
    30001 tacctattcc tgaataagcg tcgatcacaa tgtctttttt atctaactcg gcaaactcga
    30061 tggctgtttg gtacagatac tctgcttgtt gagtatttac ttgataaaat gactttgctg
    30121 agattcgata ggtttttcct aatagctgat cttcgatata gtctctgcca tataaaactt
    30181 tttctttgtc tcctaaaatc acatttgttt tttcttcatt gatattttgg atgaccgaaa
    30241 ccacttctgg caattcttca tgaatagctt cagcaatttc cttggattta aagaattttt
    30301 ctttacgagt aactaaaacg accatcattt catgggaata atgaccacga cggataatga
    30361 tgtgacgaag ctgtccttca tttttttctt cattatatcc tcgtacttgg aaccgttgca
    30421 agatatctct gactactttg attgcttgat cgatgacagg atcttggata taaaaatctt
    30481 cgatagggac tagatcatgg ctattctttt tgtaaaaacc ggtggctaac tgtccatcga
    30541 tacggcgaac agggatctgt gctttattac ggtagccgaa tggttctgcc attccgaccg
    30601 ttggaagtac aggaacttct ggcatcttag caatcttcgc cattacatgc tctacttgtt
    30661 tttgtttgaa gacaagctgt tgttcatact tcaaatgggc taaaggtgcg atacctatgc
    30721 gaagtaatgc attgttttct acaggctgac gatcaggtga ttgtgtaata aatgtttcga
    30781 cttttccata tccaaactta ttgcctgctt ttactactag tatttctact tcttctccag
    30841 ggagtgcatt ttcgataaat aatggataac catcgatttt tgctaccccc attccttcat
    30901 aactcaagtc gacgatttcg actgtatatc gttcgttttt ctttactatt tgctgttctg
    30961 tcatattttg ctcctttaag cctatttttc aatcctactg aactctacta taaatcagct
    31021 aattacacaa ctaaaaccgg cactgacctt actacgttca gcaccggctt ttgattaatt
    31081 cttttgatta ggtgtttcgt ctgctatttt tccgtcaccg tcgatatctt catccgttaa
    31141 acgttcgact tcttttacga attctttgct gacaacctct aattcttctt catctgtacc
    31201 tgtgattgct tcatcaggaa tctgatcggt attggcaaag aactcaatgt gttgatgaag
    31261 attttggaaa tgcatcggtg cttccccgcc gtattcacca tctaagttga tcattactgg
    31321 tcgattgata tcatcttctg tttcaacgaa caaactgctt gttttggtat agattattct
    31381 tggatcatcg acatgtcgac cgccattcag catgagtgct gcaatatgca agatctcaaa
    31441 gatatttgct gtcttcacaa tgattaaaga aaacttccca tcatccaatt tggcatctgg
    31501 cgcgatctgt tcgaatcctc caacagagtt cgtcagccct aggaaaaaca tcgaagcatt
    31561 tccgatatat tccccttcat catacttcaa gcgcattttg attggcttta ctcgagggag
    31621 catctctgct cctttcgcaa gataagcaag ataaccaaaa atactcttta gttctgatgg
    31681 aacttcataa gtcaattctg tcaaatagcc acctgcagcg atattgatga agtaactgtt
    31741 atcagaaagg ccgatgtcca tttttacggt ttggtgtttt tttatcactt ctgctgctgc
    31801 tttaatatta tctctaggaa tttttaatgc tcgagcatag tcgttcgttg taccagctgg
    31861 gatgatggcc attttaggac ggtgttcaag cggagcgatt ccattaacca cttcgttgat
    31921 tgtgccatcg ccaccagctg ccactactag atcaaaacct agttctgcga ctctacatgc
    31981 ttcattcttg gctgaatctg gttctggtgt agttgcatag gcgctggctt caaatcctgc
    32041 ttcttctaat gcctgtagga tatccgccaa attttttttc aataattctt ttcccgaggt
    32101 ggggttataa attacgcgtg cttttttcat tttcagcctc tatcgtttcg ctaattcatc
    32161 tttaagtagc ttattcacga caccaggatt tgcttgtcct ttagttgctt tcatgatttg
    32221 accgactagg aagccaaccg cacgatcttt tccatttttg aaatcatcga cagattgttg
    32281 gttgttatcc aaaatttcat taatgattgg cagtagtttg ttcggatcag ataattgcac
    32341 ccaaccattt ttttcaacta cttcttgggc ttctccgcca tttgtgatca attcacggaa
    32401 gacttttttg gcgatttttg agctgatcgt tccatcggcg atcaaacgaa tcattcctgc
    32461 taagttctca ggtgtcaatt ttgtctcagc taattccagt ttctcactgt tcaagtaagc
    32521 agaaacttca cccatcaacc agttagatgc ttgcttggca tctgcaccat tttccaaaac
    32581 agcttcaaaa aaatcagaca tttcttttgt aagtgtcaat accattgcgt catactctgg
    32641 aagtccaaac tcacggatat agcgttcgcg acgagaagct ggcatctcag gcaaactagc
    32701 ttttactttt tgaatccatt cgtcatcgat cacgaatttc gggatatcag gctctgggaa
    32761 gtaacgataa tcactagacc cttctttgac acgcatcaaa actgttttat tcgttgtttc
    32821 gtcaaaacgg cgtgtttctt gttggatttc tcctcctgaa agaagaactt ttgcttgacg
    32881 tttttcttca aaagctaaac cttttttcac gaaagataaa gagttcaagt ttttcaattc
    32941 tgctttcgta ccgaactctt cttgtccata agggcgaaga gaaatattgg catcacagcg
    33001 catagagcct tcttccattt tcacatcgct gacttctgta aattggataa ttgatcgcaa
    33061 tgcttctaga taagcgtatg cttcttctgg tgaacgcata tcagcttcag aaacgatttc
    33121 gatcaaaggc gtgccttgtc ggttcaaatc aacgtaagaa taaccgccaa taccatgcat
    33181 gttttttcca gcatcttctt ctaaatggac acgttcgatc cggatttttt tctttttacc
    33241 ttctacttcg atttcgatcc atccgtcatg accgattggt tgatcaaatt gggaaatttg
    33301 atatgctttt ggattatctg ggtaaaagta atttttccgg tcaaaatgag tatctttaga
    33361 gatttcacag tttaatgcca aagctgcttt catcccgaat tccagcgcag ctttattcat
    33421 tactggcagc acgcctggat aaccccagtc gatcacgttt gtattgctgt ttggttctgc
    33481 accaaaatga gctggcgctg gagagaaaat ttttgagttg gtttttaact cgacgtggac
    33541 ttctagtcca atgactgttt caaaattcat tcgtctttcc ctcctaaaat agctggttgt
    33601 tgtttatgga attctgttgc ttgttcaaac gcatatgccg cttgatacat cgttttttca
    33661 tcaaaataat taccgatgat ctgcaatcca actggtaatc cttctgataa acctgccggt
    33721 acagacatac ctggaagtcc agccaagttg acagggatcg ttaagatgtc actcatgtac
    33781 attgtgattg gatcgttaat gttctcaccg attccgaatg caactgttgg tgaagatggc
    33841 ccgatgatca agtcataatc tgcaaagact ttttcaaaat ctcgtttgat caatgtacgg
    33901 acttgtcctg cttttttgaa gtaagcgtca taataacccg cgcttaagga gaatgtacct
    33961 aacatgatac ggcgttttac ttcttcaccg aatccttctg aacgtgagtt gacatataca
    34021 tcatctaatg atttcacatt ttcagagcga taaccgtagc ggataccatc gaatcgttgc
    34081 aaattggaac ttgcttctga tgaggcaatg atataataaa cagccacacc gtattttgaa
    34141 tgaggcaagc tgacttcttc cacaacagca cctaatgaac ggaatgtttc tgctgctttc
    34201 aatacagctt cttttacgcc aggggccaca ccttcgccta agtattcttt aggtaaagca
    34261 atcttcattc ctttgatgtc gcctgtcagt ccctctgcaa aatcaggaac tgaaacgcct
    34321 gaagaagtac cgtctttttc gtcataaccg ctgatagctg tcaatgctaa tgcattatct
    34381 ttcaccgtac gggtcattgg tccgatttgg tcaagagatg aagcaaaggc aatcaaacca
    34441 aaacgtgaga cacggccata agtaggtttc atccctacga tgccgttaaa agctgctggt
    34501 tggcggatac ttcccccagt atcactacct aaagaaactg gaatttgtcc agctgcaact
    34561 gctgctgcag aaccaccaga agagccacca ggtacttttg ttgggtccca agcatttttt
    34621 gttttcttga aataagaagt ttctgtactt ccacccatag caaattcatc catgtttagc
    34681 ttaccaacag ggatcatatc tgcttgatag attttatcca tgaccgttgc atcataaatt
    34741 ggttcaaaat tgtaaaggat cttagatgca gcggttgtca agatatcttt tgttacgata
    34801 ttatccttga taccaattgg aataccagca aggacgttgc tttcatcaat ccctttttca
    34861 tctaaagctt ttgcctgttc caatgctttt tcttcgttca atgtaatgaa tgcctcgacc
    34921 tcaggctctg tttctttgat tcgttcaaat gttgtttttg tcaaatcttg tacagtgatt
    34981 tctttcgata caagcatatg atgcagttct tctagagaac gattgtgtaa ttctgtcatt
    35041 atgcgccagc ctccccatta tcgatgattg cgggtacttt gataaagccg tcttctgttt
    35101 ccggcacatt tttcaataat tcatcgcggt tccatccagc ttgcgcccga tcttgtcgca
    35161 tcacgtttac tgtttcaacc acattcgacg taaatggaac accttccgta tctacttctt
    35221 ccagcatttc aaccatatcg atgattttac ctaattggtc ggtaaaactt gcaagttctt
    35281 catctgcgaa cgcaagtttt gctaatttgg caacgtgttt cacttgttct tcactaatcg
    35341 ccatactacc ccttctttct aaacgagtta aaaactttgc tctctatttt aacacaagag
    35401 tgccgtgtct gaaattgttt attgaaattt gtctgaaaaa actcattttt cattcttcta
    35461 ctgagcgact tttagccaat gcatctgcca tttgatcttc tgtccaaact tcgattccta
    35521 attcttgtgc tttggtcagc ttgctgccag catcttctcc tgctaccacg atatccgttt
    35581 ttttcgatac gctaccagtc actttcccgc caagattttc gatcgtctct tttgcttctt
    35641 cacgagtaaa tcgtgttagt ttaccagtaa ggactactgt tttttcttta aaaggagact
    35701 cgacttcttg caattgggca tttcgaagcc ctttgtattc gaaattgacg cctgcttgtt
    35761 ttaattcatt catcaattca tggacttctt cgttactgaa ataagtgact acactgtcgg
    35821 caatgatatc accgatcgta tcaagcgcaa taatcgactc atagtcactt ttactcaatg
    35881 tttctagatc accgaaatgt tctgctaaaa tttttgctgc tttcgctccg acatgtcgga
    35941 ttcctaaccc gaaaatcaac cgctcgaccg aattatcttt gctgttgtcg attgctgtca
    36001 aaatattatt tgctgatttt tcttttattt tgtccaacgt caataactct tcttctgtca
    36061 atttgtaaag atcagcaaca tctgctacta gttttttatc atacatctgt tctagtacgc
    36121 gcggccccaa cccatcgata ttcatcgcat ttcttgaaac aaagtggttc aaaccttctt
    36181 tcatttgagc agggcattta ggattgatgc accgtaaggc tacttcttca tccaaatgga
    36241 caagttcact tccacaaact ggacaatgtg taggcaattg gtattcttcg ctgtctttag
    36301 gtcgtttatc taacacgact tgcgaaactt ctgggatgat gtctcccgct ttataaatca
    36361 gaactgtatc tttcaagcgg atatccttca acttaatata atcaccgtta tgcaggctgg
    36421 cacggcttac tgtcgttcct gctactcgga ctggtgtcat gatagctgtt ggcgttacta
    36481 cccctgttcg accgactgtc cattcgatgt tttcgattag cgtttcaact tcttctggcg
    36541 gaaacttgta agctgctgcc catctcggtg ctttgatagt aaaaccaagt tgatcttgta
    36601 gcgagaattc attcactttg atgacgattc catcgatttc atagggcaaa tccacacgtt
    36661 tgtcatgata ttcttcaatg tatgaccaca cttcatcaat cgaatgacat agacgttttt
    36721 cccgattcgt gtggaatcca attttttcta gctcttctag cgcatcatac tgggtttttg
    36781 cttgcatagg accgaaatca gctactgtgt ataaaaaagt gtccagattt cttttcgctg
    36841 tgattttaga atctaactga cgcaaagaac cagctgcggc gttccgagga ttcgcaaaga
    36901 tatcttttcc ctctgcttct ctgtcttggt tcaattggac aaatgaccgt tttggcatga
    36961 aacattcgcc tcttacttcg atgttcattg gttcttttaa tttgattggg accgaccgga
    37021 cagtttttag gttttcagtg atgttttctc ctacagttcc atccccgcga gtcgcacctc
    37081 tcacgaaatt tccatcttca taacgcaaag aaacggaaag tccatctatt tttagttcac
    37141 agcaatagtc aactacacgt cccactgctt tttgtacacg ttgatcaaaa gctatcagct
    37201 cttctttgct gaaaacgtcg ttcaaactgt aaagcggaat atcatgagtg actttttcaa
    37261 aaccttctaa tacttttccg cctactcgtt gagtgggaga atcagacgtg atcaaatcag
    37321 gatattgttc ctcgattgct actaattctg cgtattcttt atcataaaca tagtcttcca
    37381 ctgttggttt atcttctacg tagtattcgc gagaccattg gttcagtctt gtacgaagtt
    37441 cttctgctcg tttagcagct tcgtcaatcg tcatttcatg ttccatcatt taccctccac
    37501 tactaagctt tttcaattgg cgcaaaggca gccaaaagac gttttactcc ttgacttggg
    37561 aaagccacat ctagttctag atccttcgac gtcccgctta ctcggacgac tgttcctaga
    37621 ccccattttt tgtgtttgac tttatctcca ggtttccagc tgttattttc ccctccagta
    37681 gcacttttag agggaacaac cgctgtctct ggctgtttat agctatatgc tgttttttta
    37741 gcgaatacag gcgttttttt aggcgtagga cgcatgcctt cgatttccag taactcctga
    37801 tcgatttctt ctacaaaacg agaaggtcgg ttatattgcg tccgtccata taatgttctg
    37861 gagaaagcat ttgttaaata caacgcttct tctgctcgcg tgatccccac atatgcgaga
    37921 cggcgttctt cctccagttc gctttcttcc atcaatgctc gagataaagg aaaaaccccc
    37981 tcttctagac caatcaggaa tacaactgga aattctaatc ctttggctgc atgcaaagtc
    38041 atcaatgtca cttgggaagc atcttcttct aaattgtcaa tgtcggaaac aagtgccaga
    38101 tcatttaaga agaccgttag tttttcttct ggtgcatcag cgtcttcttc attttgctgt
    38161 tcgaattgtt tgtcgaattc ttgtgtgacc gttaaaaatt cctccaagtt ttcgatgcgt
    38221 gcttgtgctt ctaatgtatt ttggattttt aagtcttcta gatatccgct acgatccaaa
    38281 acctctttcg tcaattctgt cactgtcaag tatgggatca tctgagtgac ttcttggatc
    38341 atctctccaa atgcacctaa ttgctgtcct gcttttccac cgatatttgc cagatccaca
    38401 ttttgcgctg cttcaagcag tggccattca tgaagagaag caaaactgcg tagtttttcg
    38461 atagatcctg gtccgatgcc tcgtttgggc gaattaacga tacgctcaaa actgattgaa
    38521 tcttgaggat ttaccagcac attcaaatac gctaaaatat ctttgatttc tttacgatcg
    38581 tagaacttgt gccctccaac cattttataa ggaatatttg ctttcaacag agtttcttcc
    38641 attacacgag attgtgcgtt cgtccggtac aagatggcaa agtcgccgta attccgatga
    38701 ttgcttcgaa tctcttcttg catccggtct acgataaaac gggtttcgtc tcgttcatta
    38761 tctgcacggt agtaagtgat cttgtttcct tctttgtttt ctgtccacaa gttttttggt
    38821 tttcgattgc tattgttttc aatgacttgg tttgctgcgc taaggatgtt tttcgtggag
    38881 cgatagtttt gttctagcaa aattactgca gcatctggat aatctttttc aaaatccaag
    38941 atgttctgca tatctgcacc gcgccagccg taaatacttt gatctgcatc tcccacgacg
    39001 cacaggttcc taaatctacc tgccaataaa ttgactagcg tatactgtgc gtgatttgta
    39061 tcttggtact catctacatg aatataatgg aatttgtttt gataatacgt gagtgaatca
    39121 ggatgttctt caaataatcg aatcgtattc atgatcaggt cgtcaaagtc catacattga
    39181 ttatattgaa gctctttttg gtaagcagca taacattttg ccgcaatctc ttcgtaataa
    39241 ctgccttgca tttttgcaaa atcctgaggg gtctgcaggc tgttttttgc ttgagagatt
    39301 gttcctaata tacttcgagg atcatatttt ttcggatcaa tgttcagttc ttttagtatc
    39361 cgtttcatca aggtcagctg ttcagaagaa tcgatgattg taaagttccg gttatacccg
    39421 ataaaatcaa catctcttct taaaatacgc acacacatcg agtgaaaagt cgatacccag
    39481 acatcttcgc ctccagatgc gagaatcgca ttgacacgtt ctttcatttc ttttgcagcc
    39541 ttattggtaa aagtaatcgc taggatgttc caaggattga cttctttttc ctcaatcaaa
    39601 taggcaatgc ggtgtgtcag cactctcgtc tttccacttc ctgcacctgc cattagcagt
    39661 aatggtccgt cagtgtgaag aacagcttct ttctgacggg ggttcatccc gtttaataat
    39721 tcagctttgg atgacattaa caacatcctt tcttttatca atcttgttaa ttatagcatt
    39781 ttcttggctt tcattaaact agctagttca aaatcatttg tttcttttta tggatttttc
    39841 cttgtcaatt cgttgatttc gcgctaaatt aaaagaaagc gaggcgtttg aatggaaaaa
    39901 aaaggaaaaa tcaagaaatt agataagact cagatatatt ttattctaaa aggaatattt
    39961 gttggactgc tgacaggaat cgtcgttagc ctgtttcgat tatctatcga aaagctaagc
    40021 gatttagttc gaagtatcta tgaaatgagt cgggaacagc cgatttactt aattggccta
    40081 gctatttgct gcatccttgc agcatttttc gtgggttatc tggtcaaaaa tgaaccggat
    40141 atcaagggaa gcgggattcc acaagtagtg ggacagcttc gcggggaatt atcaatgaac
    40201 tggttctctg ttttatggaa aaagttcatt ggcggtgtac tttcagttgg tgcgggcctt
    40261 tttctaggta gagaaggacc ttctatacag cttggcgcat ccattggaca aggagctgga
    40321 caattgtttc ggagtccatc ttcagaagaa aagattttaa tttctagcgg tgcaagtagc
    40381 ggacttgcag cagcattcaa tgctcctatc gcaggattac tctttgtttt agaagaaatc
    40441 catcactcct tttctccact tgtttggctg acatcatttg cttcagcaat cactgctaac
    40501 ttcgtctctc tttacttttt cggcttgaga cctgttttat atctaggtga ccttccttct
    40561 ttgccgttga aatattatgg ttccttagtg atcttaggag tacttctagg catcttaggt
    40621 tttatttatc agaatgtctt gctggctttg ccaaaatttt acaaccgatt gcctttacca
    40681 gcttattttc acgggttcgt tcctttcttg ctgattttgc caattggtta tttatggcct
    40741 agtactctag gcggaggcaa tcaaatcgtt ttagcttttg gacatgcttc cttgccaatc
    40801 attatgatta tcggtttgtt tgttcttcga ttcgtttttt ccatgatttc ttacggtggg
    40861 ggtcttccag gggggatttt ccttccgata ttgacattgg gggctttaat cggtagcttt
    40921 tatggaaaca ttatggttga tttcgggatg gatccaatct acatcaagga ttttgtcgtt
    40981 attgccatgg ctggttattt cacagcgatt ggaaaagcac cgctgacagc aattatttta
    41041 gtgacagaaa tggttggctc tcttaatcat ctgatgcctt taggattggc tgctcttgtt
    41101 gcttatatcg tcaatgacct tctcggcggt aatccaattt acgaatcatt attggaacgt
    41161 ctacttggaa atactcagac cgcctcacta aaaggtgcaa ggacaacttt tgattttcca
    41221 gtaacggcag agagttcttt agacggatta atggtacgag acttcaattg gcctaaaaat
    41281 atgctgctaa tctctatacg cagaggaaat caggaaatct tgactcacgg tgatacgatc
    41341 atgaatgtag gagatatttt ggttatcttg actgatgaag caaacttgcc aagaatcaag
    41401 caggaaatcg aggctaaatc ttctatcaca aatttgaagc aatcatttta acgaacatgg
    41461 aaaaactatt ttacaacaac tattcatcta cactacttga ttctatatga ttctataaaa
    41521 aaagaagagg ctgggaaaac ccagcctctt cttttttatt tctatgcttc tgctactgca
    41581 ggagcttttc tcaataaacc ataaatcaca ccagatacga ctgctccgat agcaataaac
    41641 accaaataca tgaccgggtg actcagtaag aatacaacga aaatcccgcc gtgaggagcc
    41701 agtaatttga ttccgaaagc tccgactaat ccgccagtta aagcagaacc aataataaaa
    41761 cttggaatgg cacgaatcgg gtcagctgct gcaaatggaa tcgatccttc cgtgacaaac
    41821 gatagtccca tcacgatgtt cgtcaaacca gcatcttgat ctgttttgct gaatttattc
    41881 ttaaacaaac gtgtagcaac gaaaattgcc aatggaggta ccattcctcc agccataaca
    41941 gaagccatca cgacacttcc gccagttgca acagtcgtag ctaaagttgc tgtaccaaac
    42001 acataagcgg ctttgttgat tggaccgcct aaatctgccg ccatcattcc agctaataat
    42061 gcaccaagta aagcagcatt cgaaccactc atacttgtca agaaaccgtt caatgcatca
    42121 ttgattgctt tcatagggac attgaccagc aacatcaaga aaccagtaat cagtaagcca
    42181 aacactggat aaaagaggat cgttttaatt ccatccaatg attttggtaa gttcttgaat
    42241 aatttacgta aaaatacgat gacataacca gctaagaatc caccaatcag tgcacctaag
    42301 aatcctgcac cgcctgtatt agctaatgca ccagcagcaa atccaacgat caaaccaggg
    42361 cggtcaccga tacttgaagc aataaatcct gctaataccg gcagcatgaa accaaatgca
    42421 gcttgtccga tttggttgaa ccagctagca gcttgattgt aattccctag atttgataat
    42481 tgatcatgtg gcacgccaat aaattggtcg atcatgaaag aaagcgcaat agcgattcct
    42541 ccaccaataa cgaaaggtaa catgtgtgat acgccgttca tcaaatcttt gtaaattctt
    42601 tgtccgatag taccatccgc atttccttct tctgaactgc tttcttttcc atcaccatgg
    42661 aatactggtg cagtaccact aagggctaga ttgatcaact cttctggttt acggatacca
    42721 tcactgacag gacgattgat cagttctttt ccgtcaaagc gattcatctc tacttttttg
    42781 tctgctgcaa cgattacacc ttctgcacgg gcgatgtctt cagcagtcaa tcggtttttg
    42841 attccttctg aaccgttcgt ttcgactttg atctccacac ccatttcttt cgcttttttc
    42901 ttcaaagcat cttctgccat gtaagtgtga gcgatccctg ttggacaagc tgtaacggca
    42961 acaacgaatt ttctttctgg atgagcagaa gcttctgcag cttgtgtttc ttgttctgct
    43021 ttttcttctg cttctttttg ctcttcagct gtttggaaca agtcttggac ttcttctggt
    43081 gtatttgctt cttttaattt tcctacgaaa tctgcatcaa tcagcaatcg agatagtgct
    43141 gctaatgctt gcaaatgtgt gtcgtttgcg ccttctggag ctgcgatcat gaaaaacaga
    43201 taagttggct gtccgtctaa ggcttcataa tctactcctt tttggctttt tgcgaataaa
    43261 actgttgctt ctttaactgc actgtttttc gcgtgaggca tggcgattcc atcaccaaga
    43321 cctgtagaag tttgtgcttc gcgggctaga attccttctt tatatgtttc gatatcggaa
    43381 atccggccgg catcaaacat tttttggacc atttcatcga ttgcgccttt tttatcagtc
    43441 gcttgcaaat ccatgatcat cgcttctttg accattaaat ccttgatatt catgattatc
    43501 ctccttgaaa ctaatcaatt tttgtgattt caacttctgg cagcaactca tcaatcaatt
    43561 cgcgagctgc caagtcatca gaaaaagctg ttgcgcttcc gcaagcaact ccccatttaa
    43621 aggcttcgat tggatctttc gtacggctgt aagccccaat aaagccagca atcattgaat
    43681 cccctgcacc tactgaattt ttcaatgggc gaactaatac attcgtttta taagttcctt
    43741 gttccgtgaa gagcaaagca ccgtctccag ccattgagat cagcacattc tgtgcccctt
    43801 cagctagtaa ttttttccca tacggaatga tttcttctac agattggaag gttgtgttgt
    43861 aaagctcagc taattcgtga ttgtttggtt tgatcaacaa cggtttctta acaagtgcat
    43921 tttttaggtc atcacctgtt gtatcaatca cgaaatctgc acctttttct cgaatgatat
    43981 caatcagttc ttgataaaat ccttttcgca ggctagctgg cgcgcttcct gacaatacga
    44041 cgatatctcc ttgtcctact gttcctaatg cttgcttcaa ctcatcgatt tcttcatctg
    44101 aaatagatgg gcctaagcca ttgatctcag tttcagtgtc tgattttagt ttgatattga
    44161 ttcttgtatc ttcttttacg gttgtgaaca aagactggat ttgttctttt tcaagccact
    44221 tcgaaataaa ttcgccagtg aaccctccta aaaaccctag tgctgtagat tgagaatcga
    44281 tccgtttcaa aatacgggaa acattgatcc cttttcctcc aggtaacttg aagtcttcct
    44341 tcattcggtt caaatcgcct agttttaacc catctacgcg tacgatataa tcaattgatg
    44401 gattaagtgt tactgtgtaa atcattgtat tgcctcctta attgttgtct tttcacggaa
    44461 ttcattaacg aattctaatg gacaacgctc ggttatgatc gtggctgctt ccaaaggtgc
    44521 aacagaggtg aaggacaact ggttgaattt ggtatgatca atcaatacaa atgtctcttc
    44581 ggaatttttc atggctaaac gtttaacagc tgcctcttct ggatccggag tcgtgaatcc
    44641 ttgttcaaga tgggcaccgt tcattcccat aaatgcttta ttaaaatgaa aattttttag
    44701 ttgttctatc gcattagatc ctaaaacagc attagttgcc agctttatcg ttccacctaa
    44761 aataatggta gcaagctcca aatcaactaa atttgccgca tgttgaacgg aatttgtcac
    44821 tactgtgatc tgttttcctt gcagaaaagg aatcatttct aacgtagtag agccagcgtc
    44881 taaataaatg acatcttcgt cattaatggt ttcagctgcc gatttggcga tttgctgttt
    44941 ttcgtgaacg tttttgagtg atttttcact catattctgc tcaaaattca ataattgctc
    45001 tttcttagct ccgccatgaa ctctttctag aaacccacta ttctccagct cttgtaaatc
    45061 acgtcttatc gttgattcag aagcattcaa aaggtttaca agatcttgtg atttcacgat
    45121 tttctgctgt tcaagcaagt ctaggatttt ttgtctgcgt tcctctgtaa gcattttcac
    45181 gatcacctca atacaaataa tagcatattt tttttctact agcaatataa atcagtcaaa
    45241 aacgttcata aaaattataa atcagtcaaa aaaaaataaa aaaccagtca gaaagactgg
    45301 ttttttattt ttttgtctat catcaagccg ttttatcgtt ttttattttc gcttcttgtt
    45361 tttttcatat aaacagaagc aggttttctt tccttatgaa gaagataata ataaagtccc
    45421 atcccgccaa acatgaccaa aaaaccgatg actgatcgaa taggaaaatt aggaacaaaa
    45481 aagagacaga cggataataa tgatgccgga accattattc gataatagag ttgtttatcc
    45541 attttcttca cctcatattt tttgtaaagt aatcaattgt tctaattctc ggtaatctaa
    45601 aaattgcaca cgcagatgct gctcaccttt cttgacatcg aattggatac ttcgccgctt
    45661 tttctttatt cgtttgaagg taacatcaga gagtctatgt ttgatttcct ttaacggtaa
    45721 ttctgacagg gattctgaag gaacatagat tcttaaggat aaaagatact cttgatattt
    45781 ttcatctgtt acctctactt ctagatattc cctaatataa taacaataag cttcgattcc
    45841 attatccaaa gcaacagtcg gatcgataac tttatagcac atcatgcgtg atcgattctc
    45901 gtcaacccaa ggaaaatacg cctgagcatt tagtgttccg cgaacacgcc tttctttata
    45961 atctccttgt tggatatcat aactttttgt tacaaaaaat gaagcattct ttttaggaac
    46021 aggttgcatt tgtgcattct gatactggac agattggatc gtatggtcag tgtaaatgta
    46081 ttcaacgaat ggcggtttca ataaaccgac accattgtcc caagcaagat gatcaaaccc
    46141 aagctggtcc ctaaaatagg attgaaacat ttgattgttt ggcttttgcc agttaaaggg
    46201 gtctcgataa agctggatac atatgacttc gttttctaat tccttcactt tttctacttg
    46261 ttggatgtcg agaaaaatcg gtttagggat gatttgatga taatagcgac tataatacat
    46321 ttcccaacac gaagtgaaac ataaaccttg gtaaaaaaga tcgtatcctg gaaattggtt
    46381 gcaatcgact actacttccc cgttttgttg tttcatttta ggtaaatgtt cgatttcatc
    46441 ctttttctca aacaatagcc gttgagagat ctgttttgta ttgtgataga taaactcgct
    46501 ataagctcgc atataggcga acaccccatg agcaatcatt tttttctgaa tgaagtctcg
    46561 aatcaaaaat tgcttttctt tgaaaacaga gatagacaat acatttttta tcaataattg
    46621 attatcttcc aagcgaaaac tacattcgtt ctcgtcattt cctacaacta accaactttc
    46681 gtctcttacc gatgctagca ttattttttc ttgaagcttt cgaagatcat acggatccgc
    46741 ttgttgtgtg aaagagcgga taaatgttgg ctggatcaac agacgtgtca aaatagccca
    46801 atcgcttatt tcctgttttt tttcttcttc atttaagtct gtgagctgga tagtcatcaa
    46861 taatttatct tcgccaatca ttttctcacc tctttcaaat ctttttataa tttaattata
    46921 accaatgata aaggaaaact aaaactttat gtcttagaaa ccaatttgga cgattacatg
    46981 atagactaag taaaaagaaa caaagaggtg tttacaatgg ggttgaaatt tgaacgtaca
    47041 gataatttag ataaactttt cgaatcattt gctgtcgatc cggaaaaaac agttgcacca
    47101 aaagaagaga tcgatcgatt tctaaaacca cagccacaaa atgaaaagaa aaaagacaag
    47161 aaacaaaagt aagttaagct caaaaaaaga cttaccagcc aaacttttat gaaagagttt
    47221 gactggtaag tcttttttat tctttttatg attcaattgc ttgttcgatt gctgcccacg
    47281 cttcttcttt tccctgtttt gtaacggaag aaaagagaat aaacgtgtca ttgcgatcaa
    47341 aatccaacgc ttttttgatt tcgctctcat gcttgttcca tttaccacga gggatcttat
    47401 ctgccttagt ggcaacaaca attacaggga tatcataata cttcaaaaat tgatacatct
    47461 ggatatcttc ttgagaaggc ttatgacgca aatctactaa agaaacgacc gcacgtagct
    47521 ggtctctagt cgttaaatat gtctcgatca tctttcccca ttttgctcgc tcagttttgg
    47581 atacttttgc atacccatat cccggaacat cgacaaaatg taatgcgttc tcgatcaaat
    47641 aaaaattcaa tgtctgggtc tttcctggct taccggaagt acgggctaaa ttctttcgat
    47701 caatcaatgt gttgataaaa gaagatttcc ctacatttga tcgtcctgcc agagctattt
    47761 ctggcaattg tgtatctgga tattgttttg gtgccactgc acttatgata atttcagctt
    47821 gatggacttt catggtctct ctccttaaca aggtgatgaa taaagcaagc ggctgtcgca
    47881 aaagccaaaa tgacttttac ggcagcctct tttcatcaac ccgctttttt ctctgaatct
    47941 ttatagatta ctgttggctc accagacaat tcagctgctt cttttgtgat aatcactttt
    48001 tcgatgtttt catttgaagg gatatcaaac atcacatcca tcatgatttc ttcaatgatg
    48061 gaacgaagtc ctcgagcacc ggtattgcgt tcgattgctt ttttcgcaat ggctctcaac
    48121 gcttcaggtt cgaattcaag ttcggtatta tctagagaaa gaagtttttg atattgtttg
    48181 actagcgcat ttttcggctc agtcaagatc cgtaccagat catctgttgt caatttctct
    48241 aaagctgcca taacaggtaa tcgtccgata aactcaggga tcaatccgaa ttttagtaaa
    48301 tcttcaggaa tgatatgctg catgacactt tcgtcttcag aaagtttttg gttttgtgta
    48361 ccaaatccaa tcgtcttttc gcccatacga ttcttcacaa tcgtttcgat gccatcaaat
    48421 gcaccaccta cgatgaacaa tatgttcgtc gtatcaatct gaataaattc ttgatgtgga
    48481 tgtttacgtc cgccctgagg tggcacactt gcaacagttc cttctagaat tttcagcaag
    48541 gcttgttgga caccttcgcc ggaaacgtca cgagtaatag atacattttc gcttttacgc
    48601 gcaattttat cgatctcatc aatatagatg atcccttttt ctgcaagttc cacattgtaa
    48661 tcagcagatt gaagcaattt aagcaaaatg ttttctacat cttcccccac ataaccagct
    48721 tctgtcaaac ttgtcgcatc tgcgattgca aaaggtacat tcaatgtttt tgctaatgtt
    48781 tgtgctaaaa atgttttacc agaccctgta ggtccgatca agcagatatt gcttttttgc
    48841 agttcaacat catctgtgtt ttcttgtgct tgctgattga cacgtttata gtgattgtat
    48901 acggcaacag ctaacgagcg ttttgcgcga tcttgtccaa tcacgtagtt attcaatact
    48961 tcaagaattt ctagtggttt agggacttca gtgaattcgc gtacagcttc ttcataaaat
    49021 tcttcatcaa tgatttcttt acataggtcg atacattcat tacagatgta gacccctgga
    49081 ccagctacga tctttttcac ttcttcttgg gttttcccgc aaaaagaaca gcgtacggtc
    49141 tcattgctac ttggattgtc atacatggtc ttcacccctt aacttgcaaa aagtatgcaa
    49201 gaaactatca aagactttac agtcttttac tatcataaca tagtttagta aaaagaaaaa
    49261 gtgtttaaca aaggatcaac tgacctatac ctttgcattt tttactctta acaaaaaata
    49321 aggaactgtg acaaaatcgt gtcacagtcc cttatttcga ataaacaatt ttctagaagt
    49381 agcttcttca gaaatgattt attacttgat ttctattctt tcgcttctgt cgctgtacct
    49441 gtgatcaatt cgattgcttt tttcattgtc acatcatgtt ccagcatatc ttctgtcaac
    49501 acgcgtttga tttgttcgat tggcatgttg tatgtttctg ataattcttg gatttctgca
    49561 ttgatttctt cttctgttgc ttgaagattt tcagcagctg caattgcttc gatcacaaga
    49621 tttgttcttg tgcgcatttc tgcttcacct tcgaattgtt tatgcaagtc ttcttctgta
    49681 gaaccagtta attgatagta catttcagga gagatacctt gacgttgcat gttgttcaag
    49741 aattcgtcca ttgaacgatg tacttcatca tgaaccatca cgtgtggcaa ttcaacgatt
    49801 tctgcgtttt caacagccat acggatcgct gcttcatctt ttgcatcgtc cgctgctttt
    49861 tcttttgttt ctactaattc tttgcgatat ttttctttca attcgtctaa tgttgatact
    49921 tcgtcatcaa catcttttgc gaattcgtca tctaattcag gcacttcttt tgcttttact
    49981 tcatggattg tcactttgaa gactgcttct tttccagcaa gatcttctgc ttgataatct
    50041 tctgggaaag ttacatttac atctagactt tcacctgatt tatgaccaac taattgatct
    50101 tcaaaacctg gaatgaatga gcctgaacct aattcaagtg agtaattttc gccttttccg
    50161 ccatcaaacg cttcgccatc tacaaagcct tcgaagtcga ttactactgt atcgccattt
    50221 tcagctgcag catcttcttt gataactaat tcagcttgtg cttcttgttc acgtttgata
    50281 cgtgcatcca cgtcagcatc tgttacttca cggtcttgtt tttcaacaac tagatcttta
    50341 tattgtccta attttacttc tggtttcact gttacttcag cagtgattac ccagtcttga
    50401 cctttttcca tgctttcaac atcgattttt ggttgagaaa caggatcgat gccagcttct
    50461 ttgacagcag cttcataagc ttctggtaat acagcattca atgcatcttc atataaagct
    50521 tcttcaccgt acatacggtt gaatacttga cgagaaactt tccctttacg gaatccaggt
    50581 acgttcaagc tttttttcac tttattaaac gcagttgtta atcctttttg gatttcgtct
    50641 tgcgcaatcg taaattttaa aacaccatca ttcgtgcctt ttttttcaaa tgttgcagtc
    50701 atttaattcc ctccgagttt agaaattcat tttatacatc aaaaacgttt catgcatacc
    50761 tttaaatagt acactaaccc tttcaaattg taaacatatt gaatggatag aatccttgtt
    50821 tttcataaca tttttatctt tttcactgat aaaatctaat ggaagaagat catttgctgc
    50881 aaaatttttt ctcttttttt taaaaaatct tgtttttcat cttctgtacc ttcatacatc
    50941 gtttttcctg tacacgcctc tacagtcagt tgaaaccagc gcttcgggtc ttcgatgtat
    51001 gtatcataga caggataaac aattgcagat tgcaacgtga attcttcttt taacacggaa
    51061 ctaagcagcg catcttcatt cccgtaacga tctgaaaata cacataattg ctggtaactt
    51121 gcctgctgct gcggctttgg cagatctttt ggtatgactt tgactaactt ttcttcaatc
    51181 gtcaaataga aaacaggatg gcattcgttc aattgcctaa gtgtctccag taattttgcc
    51241 cttgctaaaa tatggacctc tggcgataca ataagtttac tcgctgtttg gataaactca
    51301 tgataaggaa gctgttcgat ctcatcaata gacgcaagct gacgtgcagg ttccaacgaa
    51361 ggaagttcct ccgatttatt gtggagtgcc ttgatcttct gcctctccat ttgtccggat
    51421 aattcttctg caaattggat tttttcaatg acccgctgct cgaaagcttc ataaaaatct
    51481 gtacttgcta tcaattttct agcatataaa aaatcttgtg tactgattaa caagtcaaaa
    51541 taaaattcag caaactcctc attttgaaga tactgttttt catgcaagag tgcttgcttc
    51601 aatgcttctt ttttctcgtc caactcgtaa agacaaaaaa caagcaggct attagcctca
    51661 aagctttctt tcaactcgta agctcgttga aataattctt ttgcctgctg atattgctgg
    51721 tttgctagtg cttgttgtgc cactcgcatt aaattctcat atttttctgg aaaatcaatc
    51781 ggcttcacgc agtgtcctcc tcttgctcat attcagctca tgttcaatag tagcaaaaca
    51841 aaaaaagcat gacaattaaa aataactgaa gcaccctcta aaagctagat tttagatctg
    51901 acttttagaa ggtgctttca actgttactg ctttttattg ctcatcagct gttgacttcc
    51961 tggttgaacg ctttgatcag attgatcgta acaacacttg tattagtgaa aaaatcgccc
    52021 agatgttgct ttttctgatt aaagactgct ggaagatacc ctaacatcag caaaggaaat
    52081 tttagaatga accggcaagc accttctcta actaaaacgg ttgaccagct aagttcttct
    52141 tcatcaaagc tgatgactcg aataccaaag atcattttcc caatcgtttg gccatgattg
    52201 aatttcgtca ataaaataaa atacgctaga taaataagta aggctgtgaa tccgtagaga
    52261 ctcagataag aatctgaaaa attaagccaa cctagatttt tggcaatacc aagtgttaaa
    52321 cgcgtaatag ctccgataca caaaagatcg accaagaagg caaacattcg gataaaaaaa
    52381 cctgcataga aatacttagg aaaatcattt gcttttggcg gttcatttct tgtgtccgta
    52441 tactttttcc atttacgttg tttctttttg atttcttcgg gcgtcaaagg ctctctttca
    52501 aaagagctca gtaccttttg ggaaaagttg gagtcaggtc ctacagcagg ctgctgttgc
    52561 ttatcttttt cttgttcctt attttcattt gaaagctctg acatgcttac tcacctccgt
    52621 aataatacat tgcttttggt gattccgctg tccctagatt ctcaagaacg gataagatct
    52681 gactagtctc agaaggcttc aatccctgga attctgctag tttacttcct aaccatgtac
    52741 tagcaaaccc agatggcgtg gaagaatatt ctactagttc cgcatcttca agctgttgtt
    52801 cctttcgtat agcttttaaa gcatcttcag gaaatcccaa ttcatcgact aaacctactt
    52861 cttttgcttg gacaccatca taaatccttc catcagcaat ttttttgact tcttcttcag
    52921 atttattccg tccctcgctg acgattgcta caaaacgact atatgcgctg tcgatatatg
    52981 cttgaagcac cgcttcttct tcttttgtct ctggtcgcgt tcctgaaccc atatctttca
    53041 acgctccgct tttaactgtt gtatcttcca cacccagttt ttctagaagt cctgaataat
    53101 tcaatcctga catgatcaca ccaattgagc cggtcactgt ttcttctgtt gcaaagattt
    53161 tatctgcttg agcagaaata tagtagccac cgctagctgc catgtttttc atgctgacat
    53221 acattgggat atcatgttct ttacgaatcg tatccaattt cttagcgatt tccgcacttt
    53281 cgtagacacc gccaccagga gaatttactt ctaaaaagat acctttgact gttggatctt
    53341 cttcaatcgc cttcagttgt tccataaaat ttttatgatt gtaaccttca gaagcaaata
    53401 aacctgaaga ccctcccgca gcaatcgttc catctactga taattttacg attttttgcg
    53461 tactgtctcc ctcttccaac acatttggag ctagttcttc atccccatat aaccaactgt
    53521 tcaatccctc aatttgatcg ttttcctgag gcttagtcaa actagctgaa actaacgaaa
    53581 ccacaaataa tcctgcagca atcagaactg ctgcccaacg tcttttattc attctttctc
    53641 ctttctaata caagtttttt atgacgatca cagtttctga ctgctcagaa gtgtcgaacc
    53701 gctctgcttt ttcttcccgc gcatacccgg aaaacgactc ttttgttaat tcatcttcta
    53761 ccgtaaactc acgagcttga gcaacttcca aggcatcagg tgcgatttct tggatgctat
    53821 cccagccagt atctaaaaat attttcgtat catctgatac accagctgat gcttcgaatt
    53881 tggacaattg tttttttaac attcccagtg tcatgatctc aatttttgaa tccattttta
    53941 ctcctcctct atccgattac tttctattgt accgaactcc tgaaaaagat gatagataaa
    54001 aacccattat tttttgacat aaaaaggaca tttgacaaag aatttgatat ataccaattt
    54061 attttttatt ctttttttgt tgacaagaac attcgcaaaa ggttatgatt ttaaatgttt
    54121 ccagataaaa aacaaaaagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    54181 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    54241 ctacagaaaa tccacaaaaa atgtctgcaa agataaaaca aaaggatttt cctgcagctg
    54301 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    54361 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    54421 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    54481 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    54541 aggtattggt catacacgat gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    54601 acatacttcc caaagtggac gcttcgtgct cgttcacaac ggcgtaatcg aaaactttga
    54661 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    54721 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    54781 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    54841 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    54901 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    54961 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    55021 catcgtagaa cgttcttcat tcgaagcaca agtagatgct tcagatatcg agaaaggtac
    55081 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    55141 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    55201 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    55261 aggtaaacag actttggaaa aattgacaca gattcctgtt gaggttcatt tagccagtga
    55321 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    55381 tggcgaaaca gcagacagcc gccaagtgct ggtaaaaatc aaccgtctcg gctacccttc
    55441 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    55501 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    55561 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    55621 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    55681 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    55741 cattggacgt ggtgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    55801 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    55861 gatcgaagaa ggaacaccag tgatcggaat catcagtgaa gaagtgactg gtgcgcatac
    55921 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    55981 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    56041 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    56101 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    56161 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    56221 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    56281 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    56341 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    56401 tgccggaaca tccatcgcac cgtttttatc acttacgcgg atattcacta atttttgcgg
    56461 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    56521 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    56581 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    56641 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    56701 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    56761 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    56821 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    56881 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccgtcat tgatatttaa
    56941 cccgtctgga tttgtgccca ttgtataaaa atctgtttca agatcggcaa acaaacgatt
    57001 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    57061 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    57121 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    57181 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    57241 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    57301 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    57361 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    57421 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    57481 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    57541 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    57601 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    57661 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    57721 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    57781 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    57841 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    57901 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    57961 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    58021 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    58081 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    58141 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    58201 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    58261 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    58321 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    58381 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    58441 agcggaactt catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    58501 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    58561 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatc
    58621 ggcacccccg taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    58681 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    58741 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    58801 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatt agttctctac
    58861 gcaagattgc caaatattct tgctgggata agccagccat cagttcatta tctaacgtga
    58921 tgctgacgtc cccggtttct tcagagacaa cgatggtaat cgcgtcactg acttcgctta
    58981 taccgacagc tgcccggtgt cgagtcccga attctttagg gatcaacata ctttctgata
    59041 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    59101 gcagcggtgt attaggaata aagatattga tcagcagctc cccagtgata tctgcatcta
    59161 aagcaatccc tgtctcaata tactcatcta atccagtatg acgttcgata gtgatcaacg
    59221 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    59281 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    59341 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    59401 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    59461 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    59521 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    59581 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    59641 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    59701 gttttaattt tagaggctga tatgctcgta tttttcttga gaaaaacgca taattttcaa
    59761 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    59821 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    59881 tttacgtcgt tctgctctgg cagctcttct agcagctctt ctttcttctg cttcaaggtc
    59941 cgtcgttttt tcttttctag tcttaacggg tacatcgata gattcctctg gttcaagtcg
    60001 tttcttgatt ttttctaatt ctcccgtcag tgcagcatag ttgtaaaaac gtgttttagc
    60061 gtcgtggaca gtcttttcaa ataaagcttt tgcaagattt ggatttgcgt tatctagcga
    60121 ggaaaagcga acttgtttcc tcatgaaatc aggcatagca gagaaatcag gttttttgta
    60181 gtccaaagtc attgggttct tgtttttttc gaccattgta gggttatatc gatataatga
    60241 ccaataacca gaagaaacag cttcttttgc ttctttaagc gattctttca ttccgccaag
    60301 caagccatgt gtgatacaag gcgtataggc aataatcaga gaaggaccag gatacttttc
    60361 tgcttcttca aatgccttga tcgtttgcat ttgattggca cctgaagcaa tttgcgccac
    60421 ataaacattt ccatatgtca tcgccatcat tcctagatct ttctttgaag catactttcc
    60481 actagccgaa aatttagcga ttgccgaagc tggtgttgct tttgaggttt gaccgccagt
    60541 attggaatag acttcgttat ccaaaacaag gatattcacg tctgctccgc tggcaagtac
    60601 atgatcgatc ccgccaaaac caatgtcgta agcccatccg tctccaccaa tcatccattg
    60661 gctgtttttt acgaatagat cctttctctt gtatatatcc tccagttcag ggaaaattga
    60721 tcgttcgctt tcgatcgcag cttgcagttt tgctgctctt tgctgcgttc cttcactctc
    60781 aaacatgtgt actaaccagt cttccatcaa ttctcgtagt tcttctgata cttttggcaa
    60841 tacttctttg attgaacttg ccaaagcctc gcgacgagtc tgtgccgcta gaagcatacc
    60901 ataaccaaat tcagcattat cttccaataa agaatttgac catgcaggcc cttgaccaca
    60961 ttcattggtc gtataaggag aagcaggaga agcaccgccc caaatagaag aacagcctgt
    61021 cgcattcgcg atcatcattc gatcaccgaa catttgtgtc aataatttta cataaggtgt
    61081 ttcgccacag cctgagcaag cgcccgagaa ttcaagaagc ggttgattga attgtgtaga
    61141 caagacagta ttctttcctt tgactggatt tgctttttgt cttaatgtca ttgcaaaagc
    61201 ccaattcaca gcttgttctt tctgttcttc ataaggcttc atcactaaag ctttttcttt
    61261 tgcgggacaa gcctctacac acagaccaca acctgtacag tcttccactg aaacttggat
    61321 acggtatttt acgccgtctg cgcctcgcat ttctctcacg ataaatcctt ctggcgcttc
    61381 ttccatttcc tcttcatctg ctaaaaatgg acggatggca gcatgaggac aaacaaaggc
    61441 acattcatta cacatcgtac agcgatcact aatccactca ggtacttcta aagcgacgcc
    61501 ccgcttttcc atagaagccg ttcctaaagg catttcgcca gttgtcattc catttgctac
    61561 aagtgtacca acagagagtt gatttccttc ctgtgcattc actggttcaa gtatttcttg
    61621 tatataacca gtgttccttt tattgatttt ttcaggcatc tccaacgttc tccaatggtc
    61681 tgggatcgcg acttgatgaa gcagttcggt cgtccgatcg attgcttgta tgtttttttc
    61741 tacgatagcc attgatttat gtccatagct tttcagggct tcttctttta gcagcggaag
    61801 cacctcatca aaaggaatga tgtcagctaa tttaaaaaaa gccgtttcca ttgccgtatt
    61861 gatccggcgt cccaaaccga cttcttgggc caatctcatg gcattgatcg tatagaatct
    61921 aatgttattt tcagccaagt aacgttttaa ttttaagggc agatgcgttt ctaattgttc
    61981 gtcgctccaa agagtattca ataaaaaaat gccacctggc ttcaatcctt tgaccaagtc
    62041 atagctatgc aggtaagctg gtgtgtgaca ggcaacaagg tccgcatgct cgacaagata
    62101 cgctgaacgt atcggcgtat ctccaaaacg aagatgagag acagtcagac cgccagattt
    62161 tttcgaatca taataaaaat agccttgcgc atatttatcg gtgtgatctc caataatctt
    62221 gatagctgat ttatttgctc caactgttcc gtcagatcca aatccccaaa acttggcctg
    62281 gaatgtttgc ggttctgtca aatccagact ttcctttggt tccaaagatt gataagtcac
    62341 atcatccacg atccctatgg taaaccgttt tttgcgtatg cttgggtctt ttttcaattc
    62401 atcaaaaact gctgagattt ggtcgggagt cacatcttta gaccccaatc cgtagcgacc
    62461 accaataaca gccgggcgaa gttcagaatc atataacgca ctttgaacgt ctaataagag
    62521 aggttctcca cctgcccctg gttctttact tcgatcaaga acagcaattg tttttactgt
    62581 atgaggcaac ttttgcacga atgtttccac tggaaaagga cgataaagat ggatattcaa
    62641 aaaacctgtt ttccgtccgt ttgcattcaa gtaatcaatc gtttgttcaa tcgtctgtgc
    62701 aactgatccc atagaaacaa tgacttcttc cgcatcgtct gctccgtaat aagtcaccaa
    62761 gtcataagaa gttcctcgta atcggttgat ctctttcata tatttttgaa cgatttcagg
    62821 taatttatca taataggggt tgatcgtttc ccgttgttga aaatggatgt ctggattctg
    62881 gttcgtacca ctaacagtcg gatgattggg attcatccct cgtttacgaa atgaagctaa
    62941 tttctcctga ttgatcagag gagctaattc ttcatattcc aaaacttcga ttttttgaat
    63001 ctcgtgactc gtacggaatc catcaaagaa attcaaaaaa gggacttctc cttcaataga
    63061 agcaagatgg gcaacaggtg ctaaatccat cacttcttgt acactgcttt cagctaacat
    63121 agcaaatcct gtttgtcttg cagccatcac gtctccgtga tctccgaaaa tattcaacgc
    63181 atttgtcgtc actgcccgac tagcaacgtg gaaaacactt ggcagtagtt ccccagcaat
    63241 cttgtacata ttcggtatca tcaaaagcag cccttgtgaa gcggtatatg tagttgtcag
    63301 tgcaccagtt ttcaatgatc catggaccac accagctgct cctgcttcgg attgcatttc
    63361 tactactttt actggttgac cgaatacgtt ttcttttcct gctgctgacc attgatctac
    63421 taattcagcc atggtcgaac tgggtgttat tggatatatt gcagcaatct cggtaaatgc
    63481 ataagaaata tatgcggctg ccgtattgcc atccattgtt ttcatttttc gcatgattca
    63541 tcatccttgt tcatattctc tcttattatc tgttccatta taacgtaaaa catgccattt
    63601 ttttctttta tttataaaaa tattttacat gtatctattt gaattggtca tctaattttt
    63661 agacaaacag tctaaatcgt tgcctttttt tgtcagacat gtataaacta taagcgaaag
    63721 aggtgtttta acatggatat atcagtgatt gatgcaacga aaacaaatac acaaaaagga
    63781 atcctttatg gttcatcgaa tgctcctaaa aaaatggtcg aattcatcaa cttagcttgt
    63841 ccgtattgcc gacaatggtt tgaagaatct tatgagctat tagaagaagc agtccaaagc
    63901 gggcaattgc agcgagtgat caaattgttc gataaagaaa aagaaagctt acagcgaggg
    63961 aacgtgatgc accggtatct cacaatcagc gatggccaaa aagcgatcaa agagatcaaa
    64021 caaatattcg atactcaaga cgagtggaaa catctttctc tccaagaagt agcggatttt
    64081 gcagtagata aactaaagct gacagaacaa aaagacgaac agctgagcca agctgtcatc
    64141 aacgaagctg agcaagcaca tattcgattt gttccaactg tcatcttggg taaagaaatc
    64201 tttgatgaat ctatttcaat agaggaatta aaagaattga tccaaacaaa ataaaaaaat
    64261 aaggagcgtg
//
LOCUS       DACCCR010000006        62794 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCCR010000006 DACCCR010000000
VERSION     DACCCR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.006"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(160..444)
                     /locus_tag="IVK93_000367"
     CDS             complement(160..444)
                     /locus_tag="IVK93_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385688.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="IVK93_000368"
     CDS             complement(462..1352)
                     /locus_tag="IVK93_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP6385689.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="IVK93_000369"
     CDS             complement(1372..1590)
                     /locus_tag="IVK93_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385690.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="IVK93_000370"
     CDS             complement(1560..3260)
                     /locus_tag="IVK93_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP6385691.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="IVK93_000371"
     CDS             3527..4300
                     /locus_tag="IVK93_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385692.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="IVK93_000372"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="IVK93_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP6385693.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="IVK93_000373"
     CDS             complement(5124..6095)
                     /locus_tag="IVK93_000373"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6385694.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="IVK93_000374"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="IVK93_000374"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP6385695.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="IVK93_000375"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="IVK93_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP6385696.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="IVK93_000376"
     CDS             complement(9050..9874)
                     /locus_tag="IVK93_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6385697.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="IVK93_000377"
     CDS             complement(9847..10527)
                     /locus_tag="IVK93_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6385698.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="IVK93_000378"
     CDS             complement(10550..11488)
                     /locus_tag="IVK93_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP6385699.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12584)
                     /locus_tag="IVK93_000379"
     CDS             complement(11733..12584)
                     /locus_tag="IVK93_000379"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP6385700.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="IVK93_000380"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="IVK93_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6385701.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..16258
                     /gene="proC"
                     /locus_tag="IVK93_000381"
     CDS             15452..16258
                     /gene="proC"
                     /locus_tag="IVK93_000381"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP6385702.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16337..17893)
                     /gene="rny"
                     /locus_tag="IVK93_000382"
     CDS             complement(16337..17893)
                     /gene="rny"
                     /locus_tag="IVK93_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP6385703.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18304..19353)
                     /gene="recA"
                     /locus_tag="IVK93_000383"
     CDS             complement(18304..19353)
                     /gene="recA"
                     /locus_tag="IVK93_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP6385704.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19462..20703)
                     /locus_tag="IVK93_000384"
     CDS             complement(19462..20703)
                     /locus_tag="IVK93_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP6385705.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="IVK93_000385"
     CDS             complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="IVK93_000385"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP6385706.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21730..22614)
                     /locus_tag="IVK93_000386"
     CDS             complement(21730..22614)
                     /locus_tag="IVK93_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6385707.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="IVK93_000387"
     CDS             complement(23392..24678)
                     /locus_tag="IVK93_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6385708.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="IVK93_000388"
     CDS             complement(24671..25939)
                     /locus_tag="IVK93_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6385709.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="IVK93_000389"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="IVK93_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP6385710.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="IVK93_000390"
     CDS             complement(26711..27157)
                     /locus_tag="IVK93_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6385711.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27250..27495)
                     /locus_tag="IVK93_000391"
     CDS             complement(27250..27495)
                     /locus_tag="IVK93_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385712.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27610..28188)
                     /locus_tag="IVK93_000392"
     CDS             complement(27610..28188)
                     /locus_tag="IVK93_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6385713.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28265..28795)
                     /locus_tag="IVK93_000393"
     CDS             complement(28265..28795)
                     /locus_tag="IVK93_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP6385714.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="IVK93_000394"
     CDS             complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="IVK93_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP6385715.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29652..30047)
                     /locus_tag="IVK93_000395"
     CDS             complement(29652..30047)
                     /locus_tag="IVK93_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6385716.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30044..31456)
                     /locus_tag="IVK93_000396"
     CDS             complement(30044..31456)
                     /locus_tag="IVK93_000396"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP6385717.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPKVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="IVK93_000397"
     CDS             complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="IVK93_000397"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP6385718.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(32311..33771)
                     /locus_tag="IVK93_000398"
     CDS             complement(32311..33771)
                     /locus_tag="IVK93_000398"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP6385719.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33941..35098)
                     /locus_tag="IVK93_000399"
     CDS             complement(33941..35098)
                     /locus_tag="IVK93_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP6385720.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35132..36502)
                     /gene="radA"
                     /locus_tag="IVK93_000400"
     CDS             complement(35132..36502)
                     /gene="radA"
                     /locus_tag="IVK93_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP6385721.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36565..37092)
                     /locus_tag="IVK93_000401"
     CDS             complement(36565..37092)
                     /locus_tag="IVK93_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP6385722.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            37322..38128
                     /gene="proB"
                     /locus_tag="IVK93_000402"
     CDS             37322..38128
                     /gene="proB"
                     /locus_tag="IVK93_000402"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP6385723.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            38125..39372
                     /locus_tag="IVK93_000403"
     CDS             38125..39372
                     /locus_tag="IVK93_000403"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP6385724.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            39448..39792
                     /locus_tag="IVK93_000404"
     CDS             39448..39792
                     /locus_tag="IVK93_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385725.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(39909..40235)
                     /locus_tag="IVK93_000405"
     CDS             complement(39909..40235)
                     /locus_tag="IVK93_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385726.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(40608..41809)
                     /locus_tag="IVK93_000406"
                     /pseudo
     CDS             complement(40608..41809)
                     /locus_tag="IVK93_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            41926..42441
                     /locus_tag="IVK93_000407"
     CDS             41926..42441
                     /locus_tag="IVK93_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6385727.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(42640..43086)
                     /locus_tag="IVK93_000408"
     CDS             complement(42640..43086)
                     /locus_tag="IVK93_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6385728.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(43133..44611)
                     /gene="msr"
                     /locus_tag="IVK93_000409"
     CDS             complement(43133..44611)
                     /gene="msr"
                     /locus_tag="IVK93_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6385729.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(44965..45594)
                     /locus_tag="IVK93_000410"
     CDS             complement(44965..45594)
                     /locus_tag="IVK93_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6385730.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(45578..46042)
                     /locus_tag="IVK93_000411"
     CDS             complement(45578..46042)
                     /locus_tag="IVK93_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6385731.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(46529..47797)
                     /locus_tag="IVK93_000412"
     CDS             complement(46529..47797)
                     /locus_tag="IVK93_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP6385732.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(48028..48765)
                     /locus_tag="IVK93_000413"
     CDS             complement(48028..48765)
                     /locus_tag="IVK93_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6385733.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(48832..49401)
                     /locus_tag="IVK93_000414"
     CDS             complement(48832..49401)
                     /locus_tag="IVK93_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP6385734.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            49529..50401
                     /locus_tag="IVK93_000415"
     CDS             49529..50401
                     /locus_tag="IVK93_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6385735.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            50743..51615
                     /locus_tag="IVK93_000416"
     CDS             50743..51615
                     /locus_tag="IVK93_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6385736.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            51612..53570
                     /locus_tag="IVK93_000417"
     CDS             51612..53570
                     /locus_tag="IVK93_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6385737.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(53821..54672)
                     /locus_tag="IVK93_000418"
     CDS             complement(53821..54672)
                     /locus_tag="IVK93_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6385738.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            54795..55262
                     /locus_tag="IVK93_000419"
     CDS             54795..55262
                     /locus_tag="IVK93_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP6385739.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(55525..56007)
                     /locus_tag="IVK93_000420"
     CDS             complement(55525..56007)
                     /locus_tag="IVK93_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6385740.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(56107..56970)
                     /locus_tag="IVK93_000421"
     CDS             complement(56107..56970)
                     /locus_tag="IVK93_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6385741.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            57568..58416
                     /locus_tag="IVK93_000422"
     CDS             57568..58416
                     /locus_tag="IVK93_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385742.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(58587..58928)
                     /locus_tag="IVK93_000423"
     CDS             complement(58587..58928)
                     /locus_tag="IVK93_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP6385743.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            59014..59721
                     /locus_tag="IVK93_000424"
     CDS             59014..59721
                     /locus_tag="IVK93_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP6385744.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            59708..60031
                     /locus_tag="IVK93_000425"
     CDS             59708..60031
                     /locus_tag="IVK93_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6385745.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(60120..61157)
                     /locus_tag="IVK93_000426"
     CDS             complement(60120..61157)
                     /locus_tag="IVK93_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6385746.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(61604..62587)
                     /locus_tag="IVK93_000427"
     CDS             complement(61604..62587)
                     /locus_tag="IVK93_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP6385747.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT        17930 a        13384 c        11152 g        20328 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     4981 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
    16261 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
    16321 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
    16381 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
    16441 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
    16501 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
    16561 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
    16621 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
    16681 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
    16741 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
    16801 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
    16861 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
    16921 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
    16981 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
    17041 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
    17101 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
    17161 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
    17221 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
    17281 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
    17341 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
    17401 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
    17461 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
    17521 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
    17581 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
    17641 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
    17701 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
    17761 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
    17821 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
    17881 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
    17941 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
    18001 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
    18061 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
    18121 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
    18181 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
    18241 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
    18301 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
    18361 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
    18421 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
    18481 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
    18541 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
    18601 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
    18661 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
    18721 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
    18781 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
    18841 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
    18901 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
    18961 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    19021 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    19081 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    19141 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    19201 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    19261 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    19321 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    19381 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    19441 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    19501 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    19561 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    19621 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    19681 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    19741 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    19801 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    19861 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    19921 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    19981 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    20041 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    20101 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    20161 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    20221 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    20281 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    20341 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    20401 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    20461 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    20521 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    20581 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    20641 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    20701 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    20761 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    20821 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    20881 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    20941 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    21001 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    21061 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    21121 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    21181 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    21241 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    21301 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    21361 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    21421 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    21481 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    21541 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    21601 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    21661 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    21721 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    21781 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    21841 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    21901 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    21961 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    22021 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    22081 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    22141 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    22201 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    22261 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    22321 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    22381 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    22441 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    22501 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    22561 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    22621 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    22681 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    22741 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    22801 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    22861 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    22921 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
    22981 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt
    23041 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg
    23101 gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa
    23161 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt
    23221 acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt
    23281 tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc
    23341 ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
    27241 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
    27301 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
    27361 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
    27421 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
    27481 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
    27541 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    27601 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    27661 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    27721 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    27781 gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt
    27841 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    27901 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    27961 tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    28021 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    28081 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    28141 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    28201 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    28261 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    28321 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    28381 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    28441 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    28501 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    28561 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    28621 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    28681 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    28741 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    28801 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    28861 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    28921 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    28981 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    29041 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    29101 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    29161 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    29221 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    29281 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    29341 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    29401 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    29461 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    29521 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    29581 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    29641 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    29701 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    29761 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    29821 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    29881 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    29941 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    30001 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    30061 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    30121 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    30181 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    30241 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    30301 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    30361 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    30421 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    30481 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    30541 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttttgga tcaacgtttt
    30601 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    30661 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    30721 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    30781 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    30841 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    30901 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    30961 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    31021 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    31081 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct
    31141 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    31201 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    31261 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    31321 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    31381 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    31441 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    31501 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    31561 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    31621 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    31681 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    31741 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    31801 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    31861 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    31921 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    31981 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    32041 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    32101 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    32161 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    32221 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    32281 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    32341 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    32401 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    32461 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    32521 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    32581 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    32641 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    32701 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    32761 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    32821 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    32881 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    32941 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    33001 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    33061 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    33121 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    33181 attataagtt ggcataccat cgcgtttttg tatgacaaag tcgccaccga tattatcgga
    33241 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    33301 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    33361 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    33421 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    33481 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    33541 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    33601 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    33661 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    33721 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    33781 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    33841 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    33901 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    33961 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    34021 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    34081 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    34141 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    34201 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    34261 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    34321 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    34381 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    34441 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    34501 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    34561 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    34621 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    34681 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    34741 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    34801 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    34861 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    34921 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    34981 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    35041 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    35101 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    35161 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    35221 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    35281 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    35341 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    35401 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    35461 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    35521 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    35581 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    35641 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    35701 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    35761 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    35821 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    35881 catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    35941 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    36001 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    36061 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    36121 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    36181 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    36241 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    36301 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    36361 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    36421 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    36481 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    36541 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    36601 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    36661 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    36721 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    36781 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    36841 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    36901 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    36961 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    37021 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    37081 tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca tatccgaggc
    37141 tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag taaaccatta
    37201 attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt acatgacttt
    37261 actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca
    37321 catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa caagtacatt
    37381 gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc
    37441 tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg taggcgttgg
    37501 tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc aagctgcggc
    37561 ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg tatataatca
    37621 acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa
    37681 tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg tgaacgaaaa
    37741 cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata atgatcagct
    37801 gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt ctgatatcga
    37861 cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc gctatatcga
    37921 taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat ttggaacagg
    37981 cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca gcgcaatgat
    38041 tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa aagaagtagg
    38101 tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca acaggcaaaa
    38161 gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct acttgttcaa
    38221 atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca taaagactta
    38281 gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca gctgacacct
    38341 gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga cgatcctatt
    38401 ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa aaagcgcgtg
    38461 ccactaggcg tgaccggcat catttacgaa tctcgtccaa atgtcacgac agatgcagct
    38521 agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga agcattcttc
    38581 tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg cgaatctcct
    38641 tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt gatgcgattg
    38701 acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca acgagtcaaa
    38761 gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt atatatcgat
    38821 aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca
    38881 tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc ttttttcttg
    38941 ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg
    39001 ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac tgaatatcta
    39061 gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc acatatcaat
    39121 cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc tagtcaacgt
    39181 ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg ttttacagac
    39241 ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt
    39301 ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg cgatggacaa
    39361 atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt ttctgtaaag
    39421 gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt ttttatttac
    39481 cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa
    39541 ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc
    39601 gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta
    39661 gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg
    39721 attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg
    39781 actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact
    39841 gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg
    39901 gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg
    39961 tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt
    40021 tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca
    40081 gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct
    40141 ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt
    40201 gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt
    40261 aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg
    40321 gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa
    40381 ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc
    40441 cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg ttcaaaagct
    40501 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt ttgtttggct
    40561 gttcttatga cttgatgcag gacagctttt tcacaacctc tgatttctta ttcttcttca
    40621 tttgtagcaa aataattacg gtgataaatg atacaacata cactgataag aacaatggca
    40681 cctgcaaaat aaaacgcata gctttcacca atatgttcac caatcacacc tgcaataaat
    40741 ggtgctaaag ctccaccgaa aaagcggaca aaactatagc tggacgaagc aacgtttcgt
    40801 tgcagacctg gtatttccat agcaatcgtc gtcaacagtg tattgataat tccttggaaa
    40861 aatcctgcta acacaatgct gatcggtaca aggactggca tagaagttcc agctcctaat
    40921 aacagcaaac aaataatgaa taagacaaat gctgctaaaa ttgtccgata agtcgtaaac
    40981 ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag ccagaagcac accccagcca
    41041 aaaaatacaa aaccgacttc taattcagaa tagccaatca ataaaaatgg cgcatacgca
    41101 agtaaagtaa agaatccgaa attatataac aatgcgatta gtccaactga acgaagttta
    41161 tgattgctta acgctttgac accttcccaa aggcctactt ttctctttgg tttctcagtt
    41221 tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa
    41281 aatggataac gccatgaacc actgcccaag accccaccga ctagagggcc aacagccatt
    41341 ccgcagccaa gtgctgcttc atacatcatg attgcttttt ctgtgttacc aactaaaata
    41401 ctgacgatcg ttgctaaagc agtagagaca aataaagcat tcccgacacc ccagccggca
    41461 cgtaacccaa tcagcattcc gatattccca gataatccgc caagaccagc gaatacaacg
    41521 atgataaaca atcctgtcat caatgttttt tcaaaccgat ccgactagat aagaaacctg
    41581 taaatagcat caccacacca gtgactaaca tgtaactagt aaataacagg gtcgtttcag
    41641 ctggcgtagc gtttaactgt gcagcaatcg tttttaagat tgggtctact agacccactc
    41701 ccataaaagc gatcatacag gtagctgcta caactagcgc tgaacttttc ccctttgttt
    41761 cttcatttgt ttgttcttgt atatctattt gattctccaa tatttccatc cctttctctc
    41821 tattagcatc atacatgatt tattttacat gtaaatatgc acatgtaaaa cgtggaaact
    41881 ttactgtgat ataataaaga aaataagacg agaggtagga tttttatgaa tactttagcc
    41941 tatatattat tatgcgtgct tttatccaaa ccacgttctg ggtatgaatt gaagcagctg
    42001 atcacaattt tttgggaagc ccatcacagt cagatctata caacgcttgc caagttagac
    42061 cagcagggtt atgtgaccgt tttggacaaa gacgagcact ctcaaaaaaa gatctaccac
    42121 ttgactgatg aaggacgtgc tcttgtagag gaatggatca aagaggaaac tcccgctcct
    42181 acgcaaaaag atgaatttct agcaaaaatc tatgcttttt cgacgctcga taaaacaaca
    42241 gctactggac tgctattcac tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa
    42301 aaaaaattga atgacctctc gctagacaag aaacaagaat ttggacgtta tgttgtgatc
    42361 cagcgcagaa ttatgctttg tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa
    42421 atgcagcagt tttttgagta agggataaac agaacctttc tagaaatatc tattgaagag
    42481 caaattatct tcaataaaag agcaatacag ttggaaaaga aaaagcgatc cgaacgacag
    42541 gccaaaacct atcgttcgga tcactatttt tctagaactg cattcaagag actatgacaa
    42601 aaagcatgcc aaggcctttt ttcaaataac agtgttccat cattcattac tttgaattgc
    42661 ttttagcaca cttcttgaaa gcgggccaac gatgatcaac tgcaacggta aagcggcaat
    42721 cacattcatt ttccaagcat tcatataagc agagaataat tgatcaggta ttccttgttc
    42781 catgatcaca ccgaataaag acatacatgt caccattcct aaaaccatca agcatgaaat
    42841 agttaagatc atttggacct tgctttctgg atttactggt agtttaaagg caattttttt
    42901 cgccagcaca ccgatgatga agacatccaa aatgaaagcc accacgaatc caggcactag
    42961 accaatcaac aaacttgata aatgaagatc ttgatgcagc cataaattat acgcgctcat
    43021 ccccaagacc atcaaaaaac acatcattgt tgtaaaaatt attccttctt ttttatttgt
    43081 aggcaatctg taccactcct ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat
    43141 ttctcgtaag tacttttttt attttctgcc cttttatttc ataaacttca tcagccgtac
    43201 gctcgacaaa gtaagaatca tgagaagtaa acaaaatcgt tcccggatat atttgcatta
    43261 gcttctctaa agcttcgatc gttgccatat caataaaatt agtcggttca tctaacagca
    43321 acacattact tggcttagta aaaagcaaag caatcgtcag acgggtcgct tctcccccac
    43381 ttaatgtaca taacggtttc gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac
    43441 tacggacaag cgattctgaa aactctgttt gtctcatgac aaaacgaatg atggattctt
    43501 cagatgtcat ttgataaccc ttttgctgat atacttgaaa gcttactttc ggagagagaa
    43561 ggattccttc tccttgtttg cggatatgtt ctaataaggt tgtcttacct gaaccatttt
    43621 caccgacaat cgcgattttt ttacctaaag aaaactgaaa atcaccattt accagtaatg
    43681 ttctcccact tctttccaat tggacatttt gtcccatgat gggatagcgg ctgtggagtt
    43741 caagagattt tggtactgga aaacggattt gtttcatctg ctgcggcttg gttgtttctc
    43801 ctattttttg gagtcttctc tctaatgttt tcgcattctt ttggatggct ttttgtacac
    43861 tgtctttttg tttagaggaa gacaaccgat ccggtctgat ttgttgcttt ttctttttac
    43921 tggacacttt ttccaacttt tccgcttgtt ttttcttttc ttggatcgat ttttccaatc
    43981 gttttttctc tttcatgatc gactctgctt ctcgtagctg agtctttttc tccaattcct
    44041 tttgagtgag ataggcacta taattccccg aaaactctcg gatttctccg tcttttactt
    44101 cccagatttt cgatgctagc tcatctagaa aatagcgatc atgactaaca aaaaccagcg
    44161 taccataata ataacgaagc tcttcaatca gtcgatcaat actttttcta tctagatggg
    44221 ttgttggctc gtctagaagc atcccttgtt cataagaaga aagaagacgt atcaactcga
    44281 tctttcgttc ttcccctccg ctccatttcc gctctgaagt ttcttccagt cttaactgga
    44341 aatgactggc taattccaaa tttaggtcct ttgcttcagc gagataggtt aattgaggca
    44401 ggtagttgaa ggtgatctct gtttggattt tcccatgatc gggaaaaagt tcaccggcta
    44461 ttagtttgag caaagtcgat ttaccgacac cattttctcc tatgatccct atacgatctt
    44521 gctgatagac acgaagagaa tcaatagata gttctagctg gttgccaaag ctgacttgta
    44581 gatttgttat atttactgct aaattttcca tatgttttac actcctattt catgtaaaac
    44641 aacaattatc gatactaaaa aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat
    44701 cttttcctac tctgaacgaa ataactatga ctaaatcacc aaatctgtca tagctttcac
    44761 ctattctact gtcgttcgaa aaaatggaca tactccatcc atgaaattgt tgtttataca
    44821 tgtcttttga atttatttag ttgttattca aaagttcgaa acgtaatttc atcgatgcag
    44881 tcatttttcc gacctccttt tttattaggt taccacacac gattcaaaaa aagcaatagc
    44941 tactgaatag taactgaaaa ccttttattc gaacgtagtg atcttttctg gtgctaaacg
    45001 aacagactcg taaccttctt ctaccgcttt tcctattgat aaaataataa ctggaacata
    45061 acggtcttta tccaaaccaa atgcctctgc caactgatct gcttcgaatc caccaattgg
    45121 atttgtttca tagccatgat cacgcgcaac taacatgaat tgcatagccg ctaagctaga
    45181 atcgactttg actacatcgt tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat
    45241 cgcagctaat tgttgatctc tgacttcttt tggcatcttc cctgattcgt aagcttggtt
    45301 atagatttct tcgccatatt cgtaacattg catatcgcca aaaatcatga ccattgcaga
    45361 agaagtatca ttttgtctcg tattgaaacg gatcaaagga cgcaagattt cttttgcttc
    45421 ctcgctttca gcgaccacaa agcgccatgg ttgcatattg acagaagaag gtgcagtcgt
    45481 cgcttctttg atcatttcca gcatttcttc gtgtgggatt ttgtatgttt catcatatac
    45541 gcgaacggat cttctaccag tcataattgt tgaaaaatca tttttttgtt ccattattat
    45601 taacctcttt cgtttctgtt gttatattgc aaagttgggt attcaattta tctaaaagtg
    45661 ttagaaattg tttcatttct acatcgctga aagccgggga tagcacggaa cctgtatctt
    45721 tatggtccat ttcacaatgc tcgattttct tctgggcttt ttctgttagc gctacataca
    45781 cttcccggtt gttctgagga tttctctgac gcgaaacata gccatttgct tccaatacct
    45841 tcagatgacg agtaactgct ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta
    45901 gcaatggatt ttcttctttc agtctcatca tgatctcgta tctagtaaga cttataccga
    45961 tattttcttc aaagatctgg acgatttttt gatcggctaa tttgatttga tataaaaggt
    46021 gactaatctc ttctaaagac ataatttcct cctttctaat aattgactcg tcaacgattg
    46081 acttgtcaat aatagaataa aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa
    46141 aacgctctca atagaaaagt atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat
    46201 gataaagaaa gcacaacgct aaaaccaaga ttattgattc ttatcaaact atagaagata
    46261 gctcttctgc agtagaaaat tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga
    46321 atcccgtcga atattagaaa ttggatcagt atgcttaaac agtaataaaa aagttatgac
    46381 aagatgttgt cacaaccttt tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat
    46441 aagttcaacc aaacaaaaaa catgaaaatc tgtttttgtt tggctgttct tactacttga
    46501 tgcagggcag ctttttcact acctcttttt aataaccata aaatcctatt ccttcatatc
    46561 tccagcccat cggtgttagc caatctcttt ccttagcact caaggtatag tgatgggtac
    46621 cagttttttc atttggatta aatagtcgat aaattggctt ggaaccacca gaataaaaac
    46681 ttagaccttc ataacgccaa ccgatttttg ttaaagagtt gatctcagat tgtgccattg
    46741 tgtaatgatg gtctccactg taaggattgt ataaacggta gacaggttgt ccgctgtttg
    46801 gtgcttgcca agcaatccct tcatattccc agcctacagt tggcagataa tttttttctc
    46861 cggcattcaa tgtatataaa tgttctcctg tatgacggtt atacagacga tacatcggag
    46921 aagttgaagt tggtgctgta tcatactgtg tcagattata ggtttcgatt acattgttca
    46981 atgtagaacc ataattaggc gcagtggcat aacgacctgt tagccaagcc gtagcatctt
    47041 tgtaagagtt cgtattgctt ttccatgcac cggaatagta gtaaacgcct ggctgaaaag
    47101 aagtcgtctt caataccgtt acgttatctt gcaatgattc tttgtacgaa ggatattttc
    47161 taaaagcggc attcaccgta atccattgcc cattcacata ttccgaagta ggcatcgtaa
    47221 cagattggcc attatagctt cctttaatcc caaatagatt gtaattaggg gcagaagcca
    47281 aagtactgtt cccccagcca ctttcaacga tggcttgagc aatcatgact gatgcatata
    47341 agtcattggc acttgccaaa ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt
    47401 gagaattagc agatgagcga gcataaaaag catagccagt atagttttcg aattcagaca
    47461 aatctgcttg atgggtatgt gtcaatgttt gcccatctgt caattggttc gtgatttctt
    47521 ctgcattctc tggattgttc tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt
    47581 ctgttgtttc agttgttggt tcttcggttg aaattgtatt ttctgagaca gtagctgact
    47641 gcaaagtact ctctgtatcg ttttcaattc ctgtttctga agaactcgat gtctgagttg
    47701 cgacagtttc tgtcgattct tcagctagca ctgttgtgtt ggcaactgct agatttccta
    47761 aaagaataac gctaaataat aaattctttg ctttcatctt ttttctcctt tgaattatga
    47821 aacttttcct aaataacagt gtaactaaaa aagtaaaagc aagtcaatgt ccatatttaa
    47881 ataacatttg tttctttttg ataaatttgc tttttctaca taaaaagacg taaatgaaga
    47941 atattcttct ccattcacgc cttaaattga tggtcgtttg aataactatt taacaagtaa
    48001 ttctctttgg atattatccg ttatggatta tacttcctgt gtcgtcggac ggataatcat
    48061 gttactgaca gacatccggt ctggcgtttc tatcgcaaat gctacagcag aagctatatc
    48121 ttcggaggtc aatccccatt ctttttgcgc ttcatgaagt tcaagagcag cctttttgtc
    48181 gctgatggtt tgatataatt cagtttgaac agctcctgga gagatgatcg tagatttgat
    48241 gttattttgc cgttgctctt gacgtaatcc ttccatgatt gcacggacag caaattttgt
    48301 tccgcaataa acggcggaat caggataaac aacatgtccc gccacagaat cagttgaaat
    48361 gatatgccca gatttttgtt tttccatgat tggaagtacg gcggaaattc catttaacac
    48421 acccatgatg ttgatatcaa gcatattttg ccattcatca cggtgacctt caactaatgg
    48481 agcggttggc atgattcctg cattattgta caatacatcg attcgaccga acttttccat
    48541 cgctaaatcg ataactgctt ggacttgtgc aaaatctctt acatctgctg tttgaatata
    48601 tagctcagct tctggtaaag attcttttat tgcttttagg cgatcttctc ggcgtgcagc
    48661 tataactagt ttagcccctt tttctgctaa tagcttcgtt gttgcttcac cgattccgct
    48721 tgaagcgccc atgatgacaa tcactttttc tgataaatca ttcattctat tctcctcctg
    48781 ttttgaaaaa tctcttgctt atactcacta tctcttattt catcttactg ttcaaataat
    48841 ccgttcgaca ggtttgacga ttttggcagg gattcctgca acgatggtat tgtctgggac
    48901 atctttcgta acagttgcat tcgctgcaac aatggcattc tcgccaatcg ttacgcctgg
    48961 cagaatagtt gctcctgctc cgatccaagc atttttcttg atgtggattg gctttactga
    49021 caagcctctt cgttcttttg gatcggttag atggttcacc gtgatgaggt tgacacgagg
    49081 accaatcaat actgaatctt ctaaacagat gccacctaag tcaacgaaca tcacttgacg
    49141 attgataaag acatttttcc ctatactaat atgtttgccg aaatctgtat aaaaaggcag
    49201 cagaacagtc accgtatcat ctactttgtc actaatgata ttttctagcc gtttgcggat
    49261 atctgctggt tcatggtacc cagtattcag cgctgctaaa ggctcttcgt tttcagcttg
    49321 gatctgatgg atcacttcaa agagcggatc cgttactcga atctctttat tgacgatttg
    49381 tttttcgatg aggcttgtca ttttgctccc tccttttatc cttactttat aacggacggc
    49441 ttatcatgtc taatacttat aaaacatact taactattcc ttttaactat acttagtggt
    49501 atgctttttt aaaaacagga gggaaaaaat ggatattcgt atactgacgt attttttagc
    49561 agtagctaga gaaaaaaata tcagccgagc agcaaaatct ctgcatatca cgcaacctac
    49621 tctctcaaaa caactgaaag atttagaaga agaacttggt gtgacacttt tcactcgggg
    49681 ctcccgagag atacagttga cggattctgg tcgatatctt tattcaaaag gaaaagaaat
    49741 cttgtcgttg gtagaaaaaa cggcgaacaa tttaacaaat gacgcaatca tctctggaga
    49801 aatctacatt ggcggaggcg aaactaaagc aatctctttc atttctgata gccttcattc
    49861 gttgatcaag gcgcatcctg atatccagtt tcatttatat agcgggaatg cagatgatat
    49921 catagacaaa ctggatagcg gattacttga ttttggactc gtaatcgaac cgacaaacaa
    49981 acaaaaatac cagtcatttc gactgccagc ccaagatcgt tggggacttt tgatacatcg
    50041 aaatcatcct ttagcaaagc aaaccgtcat ccatccgcag gaactgaaaa ctgttccttt
    50101 gatcatttcc cggcaaacgt cagtggacag tcaactagct gaatggctag gtttcagcat
    50161 agatcagtta tcgatcgtcg gaacatataa cttattgtac aatgcttctt tgatggtcga
    50221 taacgggact tttggtgcat tagctctaga tgggatcatc aatacttatg ggacggaaaa
    50281 aatattcatc ccactcgatc cgccattaca agcttcactg aatctcattt ggaaaaaaga
    50341 ccagcctctt tctcctgctg ctcgtaaatt tttagatgtc ctaacggaga aatacagtta
    50401 agtagagttt ttttccttcg ctttcagctt tttaacaacg aataagataa gcaaaagatc
    50461 caacgattaa ataacataag tttcttaatt tatttcttcc ttttatttct gataattcca
    50521 gtaaattact ataattataa aaaaagaatt gacaaaacat aactgtctat ataaaattga
    50581 ttacgttaac aataattcgt ttcatgtgac tagatcatac taggcatgaa aaatctctga
    50641 ggaacatggt ttccctagga ggtttttcgt gtctattttt gtttggccgc aatcaactgg
    50701 tctagcaaac gtgaaaacca aatcttcaaa aagggtgcgc gcatgaaaat caaaaagatc
    50761 tttaatcaaa atgcagtatt ggtagatgac ggtggacagg aaaaagtagc tattggaaaa
    50821 gggattggat ttgacagaaa aagaaatgat ttgatttttg atcgtgatgt cgaacggata
    50881 tttgtcatgg aaccagaagg tcaaatcaag ttgcaaaatt tacttaatca gattgatgaa
    50941 cgctttttat ttgcagcaga aacgatcatt gatcatgcgg aaactgtttt gatggaaaag
    51001 ttgaatgaac atgtattgat cgctttgacc gaccacattg ctttttctgc ggagaacatt
    51061 aataacggta tcgtcatccg caacaaactt ttaagagaaa tcgaagtgct ttacagtgat
    51121 gaattcagta ttgctcaatg ggcagtagag tatctgacaa aagagcttgg gattccatat
    51181 acttatgacg aagcaggata tatcgcaatc catattcaca gcggacgaag cggtcggaac
    51241 aataaccatc gcagtatccg tgaagtgacg attgtatctg acatcatcca tttagtggaa
    51301 tcagaattgg aaacagatat ccattcagaa ccattttcac tgaattattc aagactggtg
    51361 aatcatttac gactgcttct acaacgaacc catgcgcaac aatatgctgt tttagacacg
    51421 gaaatcgtag aaatggtcaa acgaaaatac ccagaaagct ataagatttc taaaaaaatc
    51481 cgtgtgttgc tgaccaaaga ataccagctc gcgatcacga aagaagaact gggttacttg
    51541 gccatccata tcgagcgttt aagaagcgca atcgtacaaa caaatgttaa caatcatgag
    51601 gaggaaaaaa attgaaagca tatctacaac gaatgggacg ttcccttcaa cttccagttg
    51661 cggtcttacc tgctgctgct ttactagtag ggatcggaaa ctggtgggca tcttatagta
    51721 acgacattgt cgctcatttt ttacaagctg gcggtaacgc agttttaggt caattacctt
    51781 tgctgtttgc cgttgggtta gcactaggga tgtcagtgga caaatcaggt gctgctgcct
    51841 tggcaggttt ggtcgcattt gaagttccag taaatgtttt aaaaactgat tcagtagcta
    51901 ctttattgaa tatcaaacca gaatcagttg atccatcttt tgctcagatc agtaacgtat
    51961 ttattggtat tattgccgga ttgattgcgg cagctcttta taatcgtttc cacgaagcaa
    52021 aattaccgat ggctttatct ttctttagcg gcaagcgtct agtgccaatc ttggctgcct
    52081 ttgtcatgtt gctgatcagt gcagtgctac tgctcgtatg gcctccggtt tactctgctt
    52141 tagtttcttt tgctaaattc attttgagtt taggctgggg tggtgcaggt ctttatggct
    52201 tcttcaaccg tttactgatc cctacaggat tgcatcaagc aatgaactcc gtgttctggt
    52261 ttgacgtagc aggaatcaat gatattggta aatttttggc ttctgaagga acaaaaggcg
    52321 taacaggaat gtatcaagct ggatttttcc caatcatgat gttcgggctt ccagcaggag
    52381 catatgctat ttacagaaat gcacgtccag aaaacaaagc aaaaacagct tccttgatgc
    52441 tagcagcagc ttttgcttca ttctttactg gtgtgacaga accacttgaa ttttcattta
    52501 tgttcgttgc ttggccatta tatgtcttgc atgcaatttt cactgggatt tctttagcag
    52561 tgagtgcatt cttccattgg acagcaggat ttgcctttag tgccggattt gtcgattact
    52621 tcctatcttt gaaaaatccg attgcgaatc agccattgat gttgatcgtc caaggattag
    52681 taacagcagt gatttactac tttggcttta actttgctat caaaaaattc catctgatga
    52741 ctcctggccg cgaggaagca gatctttcag atgacgatac tgcaacgact aattcagaca
    52801 ataaatatgc ggcacaagca agcaaaatct acgctgcatt aggcggagcc gacaatgtaa
    52861 cttcgatcga taactgtact acacgccttc gtttgcaagt caaagataca ggcctgatcg
    52921 atcaaaataa aatcaaagcc actggcgtac ctggtatgaa gatcatcgac ggaaaaaatg
    52981 cgcaagtcat cgtaggtaca gaagtacaat tcgttgcaga tgagatggca aaactacatg
    53041 gtggtgctgc agcacgacct gcacaaacaa acaccgtggt taaaacagaa acatttgccg
    53101 gtgaaacgat cgaacaagat atttacgcta tcgcaaatgg gaaactgatt ccaatcactg
    53161 aagtttcaga tgatgtattc gcggaaaaaa tgatgggtga cggctatgct gtattaccag
    53221 aaaatggaga aatcttctct ccaatcgctg gtacgatcac aaacattttc ccaacgaaac
    53281 atgctgtcgg aatccaaaca gatgctggta tcgaagtctt gcttcatatg ggcatcaaca
    53341 ctgttgactt aaaaggcgaa ccattcactt tatatgtaga agaaggtcaa aaagtcgcaa
    53401 gaggtcaatt gattgcttta gttgatcttg ccgctattca atctgcaggc aaaaacacgg
    53461 atatggtcgt tgtattcaca aatggcgaca aagtccaaag cttagaaatc gaacccgcac
    53521 gtgatgtaaa agcaaatgat aaaatcggct ctgtttccaa caaagcctga gccatcgtct
    53581 tccccagact tttttatcga taaacaaaca ggagctgaga caaaaatctt gtctcagctc
    53641 ctttttccga ataaaaacgg cgttctcctg ccaactccgc ggtaataagt ccagctaaac
    53701 gaaacatatg aagagctatt ttttatttga ctcttcttat tacttgatgc agaacagctt
    53761 tttcacaaaa tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca
    53821 ttattcctga tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa
    53881 tactttttct aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat
    53941 cgctaaaaac atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa
    54001 cgctgcatca cttgcttcaa taatttctaa ctctttacct gtcgcacgct ctaatgcctt
    54061 ggctaattct tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact
    54121 ttctactagt acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt
    54181 tccatttcct gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa
    54241 ttcattctct acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat
    54301 cattttttca gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc
    54361 aaaacttgta taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt
    54421 atgttcggct tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt
    54481 caacgcttct ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc
    54541 ttctttcagt gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg
    54601 aaccatcttt tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac
    54661 taaataatgc atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca
    54721 tttataaatt acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga
    54781 aaggatgaca actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct
    54841 atattgaaat gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg
    54901 aaacaaatgc agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct
    54961 ttacaaaaac cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac
    55021 tagatgtcta tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa
    55081 atccaaattg cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc
    55141 tgcagaaaaa ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa
    55201 tagccgtttt ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat
    55261 aattctctct actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt
    55321 ctccggggga caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg
    55381 ctgaagtcat gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg
    55441 aaaaatgcaa aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc
    55501 gctactttca cgacctctat attatcatgg tttattgact accggtatcc agatttctcc
    55561 catatatttc ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc
    55621 gtaatcttct acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa
    55681 ttcttcaggc gattctgcta aagcgcttac aacaagatac gttccttttg ggaatgggat
    55741 gattcgcgta gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt
    55801 cccgttatag ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc
    55861 atctattgtt ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc
    55921 gacaaaatcc gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg
    55981 tttttcttcg atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc
    56041 ttacataaaa ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt
    56101 ttttaactat ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc
    56161 ctctccccat tttgtaaaag agatctcggg agcttcgact aacccataat ctgaagaagg
    56221 aagccattct gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt
    56281 attaggaaaa atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg
    56341 attttctttt gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg
    56401 gtctccatca aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt
    56461 atgcatctct tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt
    56521 gactccttca aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt
    56581 cttttcgata cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg
    56641 tggaaaagat ttttgtatgc cgtatctact gatttctgaa ggcagatatc ctgaccaatc
    56701 acgaaaagct cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt
    56761 cactcttgat ccatctagaa gatcgcgatt agccaaagcc aatctacggt ttttgatata
    56821 gtccattagc gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat
    56881 tcctgtattt tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta
    56941 atcgatcaat tggttcagat ctttaagcat atttatcttc ctccttgatt gcctttttat
    57001 catattatca aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga
    57061 aaatgttgta tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat
    57121 tatctgccta tctatgtttt acctttagaa aaataaattt tggggaagac agattctaag
    57181 taaacaaagc ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta
    57241 caaagcaaat tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata
    57301 catcatacct cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt
    57361 tgtcagataa ataaaaaaca aaaaaagttc catctttaaa acacgctatt cttttacttt
    57421 ttctcacatt ctctaagaaa agaaaaggag aatgaatttt tgacaattcg agcaaaagtg
    57481 attatcaaat atgtaaaatc agctactttt ttcttaaacg tttctttata ctaaatataa
    57541 ataagtgttt catgaaagga gaactagatg aataattggc ttgagtattt tcctgaaaac
    57601 gttttggaac gcggttacag ctatcatctg cacggttttg tccgtcattt gaactataca
    57661 agcaagtact tgtccgcaac agtttctggc acggaagatt ataaggtagt gatcacttgg
    57721 gatgaaaaga ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa
    57781 cacatggctg ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc caactactca
    57841 ctttccgaac tctcgtccct tgtttcctcc gctagttcct ctcttgtacg ggagctttta
    57901 acagagattc ttatagaaca tccgcacttt atcgaacgct tcaaagttaa gatgccattt
    57961 catgcaataa attattcaga taaattaaca actatcatcc ataaatacga tcatattatc
    58021 aagaaaaaca aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa
    58081 gaagcagttg aatcgcttat tcagcaaaat gcctatcttc ccgcattcga attgatcaat
    58141 gaagtgattg ctactctcga aacattctat tgggagcctg aggatgaacg aacccttctt
    58201 ctgatagagg actgttatta tttatggaaa gaattattag cagaagcacc tcatgcagaa
    58261 aaaaggcaaa tgttctcttg gttcgtctgc caagtcgatc atactgacgc ctcttattca
    58321 aaacgctact ctataaaaat cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa
    58381 aagaaaaaaa tagataaaaa actaaagaaa cggtaaagaa agacgatttc aatgaaaaat
    58441 agcaagtaaa cgataacaac catcctaaat ccacaagaaa tacagcagac cttgctttaa
    58501 aaacacgaat catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca atattagtca
    58561 tttgactatt aggtacgcgt tttgttttat aagtcatgac aaatttcctt cgttgctgga
    58621 tagattttcc ggcattgttg atagatttta tgatactttt ctacattttg taagagaggt
    58681 tccgctgcat ctgtatattg gataaaattc tgacaacaac tttcaggtgt gtcgaaccac
    58741 cctaacccta gtgcagcaat cattgttgcg cctaatgcag gtccttcctc catacttaaa
    58801 caatagaccg ttgcatcgaa aatatcagct tggatttgta gccatgcaag atttttcgcg
    58861 cctcctccca ccgaaacaat acgtgtaaat ctgcgacccg ctagttcttt acgtttcaat
    58921 cccaccattg acaaatgctc attaaaaatt tagaatattc acaagtttaa cgacaattat
    58981 gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact
    59041 gcgatcaaag acacgttacc taccgttttc ggttatatcg gtattggact tgcatttggt
    59101 atcgttggga aagctgccgg atttcatcca ctagtcgtca cgttgatgtc cctgctggtc
    59161 tatgctggtt ctgcccaatt tatcacagtc agcatgcttg ctagtcacag cccattgctt
    59221 tccatcgttt tctcgacctt tctagtcaat tcccgaatga ttctgatgag tatgacgatt
    59281 gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac attgctgaca
    59341 gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa gttaaacttt
    59401 cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc ttctcttgtt
    59461 ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga tttcgccatc
    59521 gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa cacctctaaa
    59581 cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg actgattttt
    59641 atcccaagca acttagtgat tgtagttgta acactgattg ggtgcggatt gggggtgtgg
    59701 atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta ctttagcaac
    59761 ttggctttct agggtcctac catttgtctt actgaaaaaa ttcgatttac cgcaaccgtt
    59821 gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt tcagcagtct
    59881 atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt tggcttcagt
    59941 tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct tggcaggaat
    60001 tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta tggcaaatta
    60061 ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg tattcttgct
    60121 tatccgttga acggaccact aacaacgact tttcctaaca tatgtcctgt ttctacgtct
    60181 ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt gctcaatgta
    60241 ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt
    60301 tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa gctgatagat
    60361 tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt tcccacatgt
    60421 ccaaatggtc ggacaaggtg cttgatttga tcaaagtagc gggtaatatc gaataataca
    60481 gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa gtcgtgatga
    60541 taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt cggacttgca
    60601 gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga accgacgccc
    60661 ccggatccgt tgatcactaa gatttttttc ccgcgattgg cattttcttc tggagttaga
    60721 ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa ggctgcagct
    60781 tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac tagttggtat
    60841 tcttgattgc ttcctgctct tgttgttgta cctgcgtaaa aaacgcgatc gccgacagaa
    60901 aatttttgca cttcgtcacc tacagcaaca actttcccga ctccatcaaa acctaagata
    60961 cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg attaacagat
    61021 actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg ttctttttca
    61081 tcaataaagc tttttggatc atcgatcggc aacccttcat aaaaaccgac taccttcatt
    61141 aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc atccatacag
    61201 tgttcatttc aatatatata gtatgctatt tgttccacct tttttattca tcataattat
    61261 tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac
    61321 ttttgtcaca acctcttata aacggtgttc tcctaccaac tcttcggtca caagtcaaaa
    61381 tattcatcaa acatgagaag ctgttttttg aaattgctcc ttgtgactca gagttaaacg
    61441 gcaggctcac aacctctgat ataaacggtg ttcaatcaac tgaccctcgg tattagctca
    61501 aaaaacgaca aaatatgaag agctattttt gtttggctgt tcttattact tgatacagga
    61561 cagctttttc acgacctcta attcacggtg ttcaagcaag aaatcattgt tcactgaaaa
    61621 gcgttggatg ttctttttca aaggtctcta agatctctcg tacttcatca gtagatttcg
    61681 tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa tcctttgata tagattttaa
    61741 agaaacgatg tagtcctgtg atggaacgcg ggatgatttc tgaataatga tcttgcagat
    61801 ctagttgcag acgaagtagt ccgatcagtt ctttttctgt atgttccctc gggtcttttt
    61861 caaatgcata aggattcttg aaaatccctc ggccaatcat gatgccatca acgccatatt
    61921 tctcggccaa ttcttctcct gtctggcgat cggggatatc tccattgatc gtgattagtg
    61981 tttgtggagc gatttggtca cgtaaagcaa tgattttagg aatgacttcc caatgtgcat
    62041 ctactttact catctcttta cgcgtacgca aatggacaga aagattggct atatcttgat
    62101 gaagtaggtg actgatccat tcttccatct cagacatttc cgtatagccg atacgcgtct
    62161 tgacgctgac aggcaaaccg ccagctttag ctgcttcaat caattgtgcc gctacttctg
    62221 gacgaaggat caagccactg ccttttccac gttctgctac attagggact gggcagccca
    62281 tgttgatatc gattccttgg aagcccattt ctgccatagc aatgctcatt tctctaaaaa
    62341 attctggctt gtctccccaa atatgggcaa ccatcggctg ttcatcttct gtaaatgtta
    62401 atcttcctcg tacactgtct tttccatctg gatgacagaa actgtctgag tttgtaaact
    62461 cagtaaagaa gacatccggt gctgctgcat gctttacgac atggcgaaat acaacatctg
    62521 ttacatcttc cattggtgct aaaacaaaaa aaggctttgg cagctctttc caaaaattag
    62581 tagtcattta tttgttactc tccattctat caagcagtga agtcgcaatc tttctcaaaa
    62641 acattgtcat tatactagga aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt
    62701 ctgattgagc aaaaaaaaga ctgtgacaaa aatcttatca cagtccattt tccggaataa
    62761 atggtgttca aaagcttgca tttgcggtaa taag
//
LOCUS       DACCCR010000007        62713 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCCR010000007 DACCCR010000000
VERSION     DACCCR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.007"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(105..629)
                     /locus_tag="IVK93_000428"
     CDS             complement(105..629)
                     /locus_tag="IVK93_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6385748.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IVK93_000429"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IVK93_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP6385749.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IVK93_000430"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IVK93_000430"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP6385750.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IVK93_000431"
     CDS             complement(2351..3031)
                     /locus_tag="IVK93_000431"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6385751.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IVK93_000432"
     CDS             3157..4026
                     /locus_tag="IVK93_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6385752.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IVK93_000433"
     CDS             4052..4567
                     /locus_tag="IVK93_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385753.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IVK93_000434"
     CDS             complement(4614..5177)
                     /locus_tag="IVK93_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6385754.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IVK93_000435"
     CDS             complement(5232..5411)
                     /locus_tag="IVK93_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385755.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IVK93_000436"
     CDS             complement(5488..5997)
                     /locus_tag="IVK93_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385756.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IVK93_000437"
     CDS             complement(6093..6473)
                     /locus_tag="IVK93_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6385757.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IVK93_000438"
     CDS             6803..7552
                     /locus_tag="IVK93_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP6385758.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IVK93_000439"
     CDS             complement(7736..9013)
                     /locus_tag="IVK93_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385759.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IVK93_000440"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IVK93_000440"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP6385760.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IVK93_000441"
     CDS             complement(9935..10879)
                     /locus_tag="IVK93_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP6385761.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IVK93_000442"
     CDS             complement(10881..11567)
                     /locus_tag="IVK93_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP6385762.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IVK93_000443"
     CDS             12041..12361
                     /locus_tag="IVK93_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP6385763.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IVK93_000444"
     CDS             12515..13420
                     /locus_tag="IVK93_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6385764.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IVK93_000445"
     CDS             13417..14190
                     /locus_tag="IVK93_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6385765.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IVK93_000446"
     CDS             complement(14371..15795)
                     /locus_tag="IVK93_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6385766.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IVK93_000447"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IVK93_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6385767.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IVK93_000448"
     CDS             complement(16517..18499)
                     /locus_tag="IVK93_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6385768.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IVK93_000449"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IVK93_000449"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP6385769.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IVK93_000450"
     CDS             complement(19412..20473)
                     /locus_tag="IVK93_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6385770.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="IVK93_000451"
     CDS             complement(20684..21211)
                     /locus_tag="IVK93_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6385771.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /gene="lsa"
                     /locus_tag="IVK93_000452"
     CDS             complement(21278..22780)
                     /gene="lsa"
                     /locus_tag="IVK93_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6385772.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="IVK93_000453"
     CDS             complement(23038..24159)
                     /locus_tag="IVK93_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6385773.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24284..25225
                     /locus_tag="IVK93_000454"
     CDS             24284..25225
                     /locus_tag="IVK93_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6385774.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYNPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25375..26271)
                     /locus_tag="IVK93_000455"
     CDS             complement(25375..26271)
                     /locus_tag="IVK93_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP6385775.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26540..27880
                     /locus_tag="IVK93_000456"
     CDS             26540..27880
                     /locus_tag="IVK93_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6385776.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVIVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28098..28541
                     /locus_tag="IVK93_000457"
     CDS             28098..28541
                     /locus_tag="IVK93_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP6385777.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(28806..30224)
                     /gene="nhaC"
                     /locus_tag="IVK93_000458"
     CDS             complement(28806..30224)
                     /gene="nhaC"
                     /locus_tag="IVK93_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP6385778.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30627..31850)
                     /locus_tag="IVK93_000459"
     CDS             complement(30627..31850)
                     /locus_tag="IVK93_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP6385779.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(31881..32720)
                     /locus_tag="IVK93_000460"
     CDS             complement(31881..32720)
                     /locus_tag="IVK93_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP6385780.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32737..33417)
                     /locus_tag="IVK93_000461"
     CDS             complement(32737..33417)
                     /locus_tag="IVK93_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6385781.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33419..34492)
                     /locus_tag="IVK93_000462"
     CDS             complement(33419..34492)
                     /locus_tag="IVK93_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6385782.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     gene            complement(35138..35809)
                     /locus_tag="IVK93_000463"
     CDS             complement(35138..35809)
                     /locus_tag="IVK93_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6385783.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(35896..38574)
                     /gene="mgtA"
                     /locus_tag="IVK93_000464"
     CDS             complement(35896..38574)
                     /gene="mgtA"
                     /locus_tag="IVK93_000464"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP6385784.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVDGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            39233..39670
                     /gene="ndk"
                     /locus_tag="IVK93_000465"
     CDS             39233..39670
                     /gene="ndk"
                     /locus_tag="IVK93_000465"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP6385785.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39692..40558)
                     /locus_tag="IVK93_000466"
     CDS             complement(39692..40558)
                     /locus_tag="IVK93_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6385786.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40718..42811)
                     /locus_tag="IVK93_000467"
     CDS             complement(40718..42811)
                     /locus_tag="IVK93_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6385787.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
     gene            complement(43287..43910)
                     /locus_tag="IVK93_000468"
     CDS             complement(43287..43910)
                     /locus_tag="IVK93_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385788.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44069..45466)
                     /locus_tag="IVK93_000469"
     CDS             complement(44069..45466)
                     /locus_tag="IVK93_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6385789.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(45498..46469)
                     /locus_tag="IVK93_000470"
     CDS             complement(45498..46469)
                     /locus_tag="IVK93_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6385790.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            46843..47268
                     /locus_tag="IVK93_000471"
     CDS             46843..47268
                     /locus_tag="IVK93_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385791.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            complement(47756..48457)
                     /locus_tag="IVK93_000472"
     CDS             complement(47756..48457)
                     /locus_tag="IVK93_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6385792.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48473..48913)
                     /locus_tag="IVK93_000473"
     CDS             complement(48473..48913)
                     /locus_tag="IVK93_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6385793.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(48913..49488)
                     /locus_tag="IVK93_000474"
     CDS             complement(48913..49488)
                     /locus_tag="IVK93_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP6385794.1"
                     /translation="MKFTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(50335..50814)
                     /locus_tag="IVK93_000475"
                     /pseudo
     CDS             complement(50335..50814)
                     /locus_tag="IVK93_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(50973..51674)
                     /locus_tag="IVK93_000476"
     CDS             complement(50973..51674)
                     /locus_tag="IVK93_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6385795.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(51782..53224)
                     /locus_tag="IVK93_000477"
     CDS             complement(51782..53224)
                     /locus_tag="IVK93_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6385796.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(53238..55103)
                     /locus_tag="IVK93_000478"
                     /pseudo
     CDS             complement(53238..55103)
                     /locus_tag="IVK93_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(55224..56076)
                     /locus_tag="IVK93_000479"
                     /pseudo
     CDS             complement(55224..56076)
                     /locus_tag="IVK93_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(56386..57060)
                     /locus_tag="IVK93_000480"
     CDS             complement(56386..57060)
                     /locus_tag="IVK93_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6385797.1"
                     /translation="MLLFLLLLVGLALIFNEQIKDYLVRETGDKYAIANVTKEDLKKN
                     NDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINLPIFKGLDNTSLLY
                     GAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLTDLENVYTYKTFFK
                     EKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTPIKDATDDMRSAFN
                     LETKTF"
     gene            complement(57166..57492)
                     /locus_tag="IVK93_000481"
     CDS             complement(57166..57492)
                     /locus_tag="IVK93_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6385798.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            complement(57614..57862)
                     /locus_tag="IVK93_000482"
                     /pseudo
     CDS             complement(57614..57862)
                     /locus_tag="IVK93_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(58163..59416)
                     /locus_tag="IVK93_000483"
     CDS             complement(58163..59416)
                     /locus_tag="IVK93_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6385799.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            59477..60217
                     /locus_tag="IVK93_000484"
     CDS             59477..60217
                     /locus_tag="IVK93_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6385800.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(60303..61061)
                     /locus_tag="IVK93_000485"
     CDS             complement(60303..61061)
                     /locus_tag="IVK93_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6385801.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(61058..62086)
                     /locus_tag="IVK93_000486"
     CDS             complement(61058..62086)
                     /locus_tag="IVK93_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP6385802.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            complement(62106..>62713)
                     /locus_tag="IVK93_000487"
     CDS             complement(62106..>62713)
                     /locus_tag="IVK93_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385803.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
BASE COUNT        18053 a        13120 c        10819 g        20721 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcagtaa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacgatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc ttttaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gtgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tatcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgcac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctgc gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacat
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatgac aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcgtacata actaatgctc agatgttttg
    21661 gctgggattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaga gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tgtggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttgataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtatt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata aaagatccat cccatcaatg gtacgccatg ttcccccgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgataaa ggagagaaaa aacatgaaaa tcgcaatagc
    24301 tggagccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg gaaatgaggt
    24361 cctcttgatc gatcgttggc ctgcacacat tgaagccatt cgtacaaatg ggttgattgc
    24421 tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag aagaaatcat
    24481 cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagcgaatc aattagatga
    24541 tatgtttcgt tcgatccaat ccattattac tgacaatact tatgttttgt gtttactaaa
    24601 tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata tcttatttgg
    24661 gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt taggtgatgg
    24721 agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa aagttgtgga
    24781 agtctttcaa gaagcaaaat taaatccgat ctatagccat aatgttcgtt attccatttg
    24841 gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg attgtaatat
    24901 tgctgaacta ggcgcgcaaa aaacggcaga gagcatggtt cgaacaatcg tatctgaatt
    24961 tgcttctatc gcagcaaaag aaggaatcat attagatcaa gaagaagtgt accaacatat
    25021 tgcttctact tataatcctg acaacatcgg gttgcattac ccatctatgt accaagacct
    25081 gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccgttt ggcgaaaagg
    25141 acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag tccatgcaaa
    25201 agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg aatcagcagc
    25261 tggttctctt tttttaagcc caatgaaaga tcaaaaagaa aacacaactc tcatgatgcc
    25321 ataagaattg tgcttgtttg tttcggtaca taatctcttc aaaatgtagg ttttttattt
    25381 attttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat cgttagtgat
    25441 aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgatg ggattggctt
    25501 aaaaggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa gcaaaaaaat
    25561 attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca ctagtttctt
    25621 ttgaaaatac atagattcaa atgtccgcaa agtcactcct tgctgaccct cccgcaacag
    25681 ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag caagatatcc
    25741 aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat ctctttttat
    25801 aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg caccaaaaaa
    25861 tagatccgtc tttggttcgc tagaagaaaa atccatatgt ttcgaataaa atgaactatt
    25921 atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct catcgattgc
    25981 cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt gattccaaaa
    26041 aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct ccatattaaa
    26101 tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag ctgatttaat
    26161 gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga tggaaggcaa
    26221 aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca tcttttccgc
    26281 ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa agccagtaaa
    26341 acgcttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg aaataaatca
    26401 ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct gaaaatttgt
    26461 tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg attgtgaatt
    26521 taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta gctccagaca
    26581 tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag gaagtggcga
    26641 agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggcg ggctgatttt
    26701 atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca cgcatcacgg
    26761 attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta ctgattgggt
    26821 ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg cttccttttt
    26881 gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg ctgttttaac
    26941 tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt ggctggcttt
    27001 tgctaaaatc agtgtcatta tcttactaat catttttgct acttatcttg tcggtcagca
    27061 aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg gtgggtttac
    27121 tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt cttacggcgg
    27181 ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg cgattcctaa
    27241 agccatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt tgtttttgct
    27301 tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt ttgtgattgt
    27361 atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtta ttgtccttgc
    27421 tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt tcttccgttt
    27481 gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc aagtaccgca
    27541 acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt cttattttgt
    27601 cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat tattgatctg
    27661 gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat tatgggataa
    27721 aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta tcctgttcac
    27781 gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt atttcagtta
    27841 tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga aaattttatt
    27901 ttatttatgt ggaatagatg gaactattga agaaaggagt tgtgacaaaa gtcttgttgc
    27961 aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt caaaaaccct
    28021 ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa ggaatgaaaa
    28081 aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc ggtgtttcaa
    28141 cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat atcttcttca
    28201 gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc atcatgctgt
    28261 tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta gccgctagtc
    28321 ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc aacgacacag
    28381 cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctgacaagt aaacgagctg
    28441 atttaaaaga tcctgaagta aaaaaattag cggaagaaat catccaggca caagaagaag
    28501 agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc gtccaaataa
    28561 aaaaagagga tgttccagaa gtgatcagct tcaagaaata agaagaagct acttctggaa
    28621 caccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga acagccaaac
    28681 aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg caagcttttg
    28741 aacaccgttt attcgtaaaa gggattgtga caaaaaaagt cttgtcacaa tcccttttta
    28801 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    28861 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    28921 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    28981 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29041 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29101 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29161 gttgtaaaaa tcaattttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29221 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29281 acagtaggca tcatactttc gattcctccg cgactaagca gtgaatcgat ttgttggttt
    29341 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29401 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgtcaaaatt
    29461 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29521 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29581 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccggaaca
    29641 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29701 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    29761 gctccaacaa ccagcgctgg atttaggcct agtgttgtcc caataccgaa aaatgcaatc
    29821 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    29881 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    29941 gttggaatga tccctgcttg tatccataca gcaatcaatg aacctactaa aataaaaata
    30001 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30061 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30121 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30181 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30241 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30301 gctattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30361 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30421 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30481 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtatgaagtt taccttccat
    30541 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30601 actttcacac acaagtttat tttttactag aaagtagcac cctcttgttt ttttcttgct
    30661 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30721 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    30781 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    30841 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    30901 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    30961 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31021 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31081 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31141 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31201 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31261 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31321 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31381 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31441 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31501 tcaataaatt gctccactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31561 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtgggt
    31621 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31681 tctacccaaa acccttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31741 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    31801 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    31861 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    31921 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    31981 ttttgatacg ttttattttt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32041 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32101 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32161 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32221 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32281 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32341 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32401 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32461 aaagaattca aatcaatcga tccatctgct aaagccgcat ttggttgatt gtaatctgta
    32521 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32581 acatctgtat cttctccaat aattcctagt tttacggtag tttctttttt gtcagttgta
    32641 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32701 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    32761 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    32821 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    32881 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    32941 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaataa taccgattgg
    33001 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33061 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33121 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33181 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33241 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccgat
    33301 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33361 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33421 atcttcctcc ttcgatcact ttgatacgac tatctaattc gtttttttgt aaaatattta
    33481 tttttacacc agatttgatc aaataatcca atgctttttg ccgctgtttg gtttctccag
    33541 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33601 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33661 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33721 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    33781 tggtcaattc ttcttcagga ttgctgaaga tctggatgct actacctttt tcaatgattc
    33841 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    33901 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    33961 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34021 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34081 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34141 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34201 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34261 gcaattcttt gggagagagc tgactcaaat tcgtctgatt gacaatgact tctcctgctg
    34321 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34381 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34441 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34501 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34561 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34621 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34681 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34741 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    34801 tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta ccaccttttt
    34861 tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg ttcgttttaa
    34921 cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag gccattttca
    34981 gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt ccaaacttac
    35041 tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga ggaaaactaa
    35101 aaagtcaagc acgaaattca aattattttt tctatattta tttttctgtt tttttatcta
    35161 acatttcaat aacagttcga agatcttttc cttcagcaag aagtttcaat ttttcctttt
    35221 cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg cctaatcctg
    35281 ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct gaactagctt
    35341 ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg ttctccgaca
    35401 cttcgtctcc gaatgtaaac gtgtcaaagt aatcagctaa tttaaagtga tccaagatat
    35461 ctgttgctct tttcgtaaca gtggtcgtag caatgcctag tttcatatga cgttctttta
    35521 agtctgccaa tatttctttt gcatatggtt ttaagtggat ggctccgctt gtcagctgtt
    35581 gataaaaata ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg ctacctgctg
    35641 ctttcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg tcaacgattt
    35701 cttttgggat atggatatca aattgctgaa atccccaaag ccagccatca cgatacgttt
    35761 tttctgtgtc aactaacaaa ccatctaaat caaatattgc acctttcatc tatacattaa
    35821 tcctttccaa aacaatacac atagtttagc acgactggtc caaaaataac agaagcaaag
    35881 aaccagcctt ctcgtttata agagttcttg gaatcgatgg atatagattt tcttcacagc
    35941 tgtcacaagc gccagataag cgatgactgt agcaaataac cataaccagt aattgccagg
    36001 aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag aaccgacaat
    36061 aattcctaat gtagtgataa caaacatggc aaatgttacg ttgctttgga taaatggtac
    36121 tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt cgacaaacca
    36181 tcctgcatgg aataatgcga tgaaaatcac ttgctgtgaa ctatccaatg tatggaaact
    36241 tccgtcaact acagcaggac aaataacaaa atacatcaac aagtaagtag taatatcaaa
    36301 tatcgaactt gttgggccaa agtaaaccat gaatttccca atactagatg cattccattt
    36361 ttttggctcg tccaaatatt ctttatccat ccgatcccat ggcaaggaaa tgcaggaaac
    36421 atcatagatt agattcaaaa acagaagttg taaaggcagc attggtaaaa atggcaggaa
    36481 catacttgca atcagcacgg aaaacatatt gccaaaatta gaacttgctg tagccttgat
    36541 atatttcatg atattcccaa aagtttctcg tccagaaaga aggcctcttt ccaaaatcgt
    36601 caaatctttt tctaaaagga tcacgtctgc agattctttc gcaatatcca cagctgtatc
    36661 tacagaaata ccgatatcag ccactttcat tgcaggagcg tcattgatac catctcctaa
    36721 aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt tttgctgagg
    36781 attcaatttt acgaatactt catggtcttc agctacttgt gctaattcat gatcactcat
    36841 ttccgagatt ttctcaccgg taaccaattc attgatctct agacctactt gacggcacac
    36901 tgaacgtgta actaattcgt tgtccccagt cagaactttt acagctacac catgttcttt
    36961 cagagctttc aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag taagataacc
    37021 aatcaaaacc atgtctgact catctttgac tgaaaattct ccaacgacgc ttggattggt
    37081 cttttgtgcg acaccaatca ctcgtaagcc atcttcatta agtgactgta cttttgcaag
    37141 tacggcttct tttcgttctt ttgttaatgg tgtaacagta ccgccaagat caataaattt
    37201 agaaatgttc agcatttctt caatcgcgcc ttttgtgatc atttgtgttt taccagctgg
    37261 atcagcaacc acaacactca tacgacgacg ttcaaaatca aaaggaattt catccacttt
    37321 ttgataattg atcttatctg tatctaaagt ttctgttgct tcatcaatga tagccacatc
    37381 taacaaattt ttcagccctg tttgatagta gctatttaga tacgcatggc gcaaaacacg
    37441 atcatcttca tttccttcac aatccatatg atattcaaga atgattttat cttgtgtcaa
    37501 tgtccccgtt ttatccgtgc ataacacatc gattgcacca aagttttgga tggcattcaa
    37561 atttttgatc actgttcctt ttttggccat cacacttgct cctttgacca aattcgtcgt
    37621 aacgatcatt ggaagcattt caggtgttag accaactgct acagatagtc caaaaagaaa
    37681 agcttctagc caatcccctt ttgtaagccc attgatgacg ataactgctg gagccatcaa
    37741 tgacataaat ttgatcagta gcatggatgt tttatggatg cctaattcaa agcttgattg
    37801 aattggtttt tcagatagcg tttctgctac atgaccaaat agtgtttcat ttccaacaga
    37861 aacaatgatt ccttccgcac ttccacttat gacattactt cccataaaga caagattttc
    37921 gtaacttgtt tcgctttgct gatcgtcttc acatactttc gcatgctttt ctactggata
    37981 gctttcccct gtcaatgctg cttgggaaat gaataaatcc tttgcggaca ataaacgcat
    38041 atccgctggt atcatatccc ctgctgaaag ccgaacaaga tctccacaga cgatttcttc
    38101 cataggaatc tctgtgtact ttccattacg ttttacagct gccgttactt tcacaagtga
    38161 tttcagtttt tcagccgctt gattggaacg tacggactga atcaacgtca ttgtcccgct
    38221 gacgatgacc atagtgaaga taatggctac accgaaaaga tctttatctc caggagctgc
    38281 taatacatat tccgtgatga atgaaataat tcctaatgcg atcaacacta atgtaaatgg
    38341 cgtgatgtat gctttcaata tttctacgat gaatggtgtt tttttgccat atgaaatcgc
    38401 attttcaccg taattttctc ttgaacgttc gatttgttct tctgtcaatc catttgctga
    38461 tgcgtggaat agttcaaata attcttgttt cgtttttcca gcaaaagctg catagatttt
    38521 ttgtgtatct ttcttttttt ccatctcaat ctctcctttg tgtccaaaag acagctgttc
    38581 tcgctgcttt tggtctttag ctattcatcg gatcgataga gaaaaaactg aaaggggctg
    38641 agaacagata gataatagcc ttggcctttc ctctagatcc ttgtttttcg aatgactgtc
    38701 atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc aatcattttt
    38761 cgaatagtta ttttcccaat tagaccacct cctttttatt tttcaccgat ggggatttgc
    38821 ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc atattaaaaa
    38881 aagcttctca gatctcatcg ttgagttttg acactgtaca acgtaaaaag cagatacctg
    38941 cttttagcta ccttatgaaa agccttatct cgacaattcc tgttctaccc attggcgtct
    39001 ctcgacgttt ttgggcagta gcctatgttt gtataggagc ctcacctaac aaatcttatt
    39061 cggttttcct tcgttaatat aacaactaaa aaagaggatg tcaaatcaat tttccatgtt
    39121 tttcatactt tttttaaaat acacacgtta tattcctgtc tcacgcggtt ttatttgtct
    39181 cattttccac ctcccgctat aatagaaata tagtaggaaa cgaggtgctg ttatggaaag
    39241 aacacttgtt attatcaagc cagacggcgt gagacgccat ttagtcggaa gcatcattca
    39301 acgttttgaa gcaaagggac tggccattgc tgaaatgaag tttgaaacta tgactcctga
    39361 attagcaaaa gaacattatc agcacttgac tgagcgcagt ttttttgatg aactgatcga
    39421 ttacatgact tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag tgatcgatat
    39481 cgtccgtaag atggttgggg caacaaaagc tgtcgatgct gttccaggta cgatccgagg
    39541 agactacgcg ctgccgggga ctgaaaacat cattcacgct tctgattccc gtgatgctgc
    39601 ggtaaaagag atcgcccgct ttttcccgcc attcgatata aacaaaccgt ctgaaaaagc
    39661 ggcgaagtga tacaaattct aaactgctgg tctagttgct gggttcagca atggccagca
    39721 gttttttctc taaggacctc cgttgattct ttcctaacat atattcgagc actgtgaaga
    39781 attcttggta gtctttcgtc tttttttccc ctaaagcagc agaaatcaat ctttgataat
    39841 aatctgccat caatgtttca tagatcactg tacttctttc tttatgtgct aagctccgtt
    39901 tcatatcttc attcacacta cgtgcctttt tcaaccgacc attttctata taaaacgaag
    39961 tcaggttcat taaaaaagta aagagatact gctcatgatt gttcaactgt gctaatcgtc
    40021 gcattctttt tttgattttc aaataattgt tctcaatgaa ccgatcactg aatatccaag
    40081 gaaaatcagc tagaatagat agttcaaatt gaccatagtc tggtatctgc atcaagtatt
    40141 cttcaatgat tcggcattca taacgtaaaa attcaaattc tgtctcagat acttctgttt
    40201 tttcgaccat gtgaagactc aaaaccaaat aatacagatc ttctgttttc ttaaaatgcc
    40261 tacggttatt gctgatctct ttataaggat caaagctgtc ttcttgtacc cgatctagca
    40321 ttcgtctttt tttctttaac tttattggtt catccagctg gtttcgcatc cattcatatt
    40381 ctgcaagtgt catgttcatt cgctctaaaa atgatctcaa tgttacaaat gacatttttt
    40441 tcactgaatt tgtctcaatc ccgactagag aagtcttaga acagattcct tccgctaatt
    40501 gggactgtgt caacttcttg ttgactcgaa gtttcttgat caattcacca tcatacataa
    40561 tagttctcct ttttaattta aatttttcca cattcaataa atagatacgt taaaaaggaa
    40621 aaatacacga aaaaaataaa aaaatgtttt tttcataaaa aataccaacc ttcaagcatt
    40681 agaatgttgt ctaactactg aaggttggtt tgattgttta ttgattatct ttttgctgtt
    40741 caacgtctgt tcttgttact ggatataagt tggttgttgt tttattgcca ttttttgaaa
    40801 agacacttgt cgaatgttcg cctttttgct tttcgatggc tatgttacgt tctgcttcat
    40861 ttttatagtc atattttttc ggatcgatcg tttcaaatcc gtttggcaca taaaaacgca
    40921 agagattttc ttgattcagc ttatctgaaa gacttaaagc agatttgact ttttcctgat
    40981 ctttggaaat ttcctcgtcg attcctgctg ctttcgtatc gatgatttct cctgtttggt
    41041 tattgtaagc ttttcctcct aagatggtgt atttgggtgt cacgaagttc ccatttcgaa
    41101 aagccactac ttgatcatgc tgaggagata ataaatccgt accaaaatgg agatagtctt
    41161 tatcatcgat tcccaagaga tgaagtaatg tcggcaaaac atcgatttct ccaccgtatt
    41221 cgtgattgac tgacccttta gtataaccag gtatatgaat cattaatggc acacgctgca
    41281 tttgtgcgtt gtcaaattca ttccatgtat ctgggtcttt tccaagagca cttgctaagt
    41341 ctttattatc tgtatttgag atgccaaaat gatccccata aatcacaaag atcgaatttt
    41401 ggtaaatagc ggattttttc aggtacgtaa aaaattgttc caatgactga tccaaataat
    41461 gtgccgttcg aacatagtta tctactgtgc tatttccagt gttcaaagat ggaaaatcga
    41521 aattcttatc atccgtataa taaggcgtgt gattggttac ggaaaggaat ttcgtgtaga
    41581 acggctgttg tagatgttca agatattggg cagattcact gaataaatct ttatctaata
    41641 ttccataacc tagcgtttca tctgattcat caaaataaga tcgatcaaag aaattgtcat
    41701 aacctaagtt tttatataca tgatctctat tccaaaaact agctacattc ccatggaaaa
    41761 cagcgcttgt atagccttgc ttttgtccaa ggatcgctgg tgcggcttga aagacattat
    41821 ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca
    41881 tattttcagc atcacttgtc tttccctgtc ctacttcatg aaaaaaatta tcaaagctga
    41941 tcgttgcttg attctggaaa atactattta aaaacggtgt gacttcttgt ccgtctacct
    42001 tcatattaat caaaaactgc tggaaacttt ctaaatggag aacgatcaca tttttccctt
    42061 tagcgatccc aaatgtttcg ggatttggct ctgcataatg tttctttgta taatctaaaa
    42121 cttcatcaat cccattactg cttgcatgcg cgcgaacact gctagtcatt cctgttttga
    42181 ttccatcata aatagtatac gcatcgattc ctagatattt gacaatatat gaacggtcaa
    42241 atgttcgctg cagcaattgt ggacggttgg cttcgctcag cgctagattt cctgaaaaaa
    42301 taagccctga aagacaggtc acagcgatag ccatcggttt gctgacaggt tgacttttga
    42361 taggtatttt cttgatcgtc aaccaaacca gtatcccaat gaacgtagca atatccagcc
    42421 agtagaggac atcatggggt ttcatcaaag aaaaggagct tcctgataac ccttgcgaca
    42481 ctttcgaaaa tcccaataca gatttgactg taatgaaatc tgtgaattcc cgataaaaaa
    42541 taatgttcaa gtacaacaaa agcgtgttta aaatatcaat gatcaaaaga atggctaatg
    42601 ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta gttgcaatcg
    42661 ggttgatcca caagataaag tactggatcg taccttccac acctaacgaa aaatctgcat
    42721 agtaagcaat aatcgtcttc atccagaaaa agaaaacagc tgtcaaaacg attgctaaac
    42781 gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat tcacattttt
    42841 ccttattttg ctcctgaaaa atgaaaattc cctaaaaaag ttgttatctt aagatttttt
    42901 tattctttct aaagattatt ccgtgcactt ttcacagcat aaaaccgctg tgatataagc
    42961 gattgaaaca gtaaggtgat aaaagtcttg tcgcagctcc tgtttagttg atgcaggaca
    43021 gctttttcac aacttcttct ttaaacggtg ttctcctgcc aactcctcgg ccacaagtca
    43081 caatatttat aaaacatgag aagctgtttt ttgaaattgt tccttgtggc tcagagttaa
    43141 acggcaagct caagacctct tgatacacgg tgttcaatca gctgaccttc ggtattagct
    43201 caaaaaacgg caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa
    43261 aacgctgatt tcacaacctc tgtttttcat tgtccatagg attgatattg tccttgtgat
    43321 gactcattcg taataattga ggcttgattt tcttcacttc ccgttatcgt ttgtccactg
    43381 ccagaatctg aaggacttgt tcctgtcgac tcgttttttt gagaagagac agttgttttc
    43441 ttgacttgtt catttgtctt tttccattcc gctaaacgat cggcgaccac ttttttgtct
    43501 actttttcag aataagcggt gatcactttt ccatttttca aacgggctac tttgtcgcca
    43561 tcatgagtca aaagcaaaac ggtcaaatgc ttgtctttaa attgatattg tctgagagaa
    43621 atcgcactaa gtacgtcatc gatattaata aattgatttt cttcttttcc ttttggcaaa
    43681 tagatgtcca attcgccttt ttcgtaattg gcagttattt tatactccgc ccaccaataa
    43741 gttgagaatt cttttttgaa attatctact gattctttgc ttgctacgat tttttctgtt
    43801 tctttctgag caagatctgc tgtcattttt cctgaaaatg ctgcggataa aataagcagt
    43861 aagaaaaaac cgaataacca aaagagaaga aacggtcttt ttttcctcat gctttcctcc
    43921 actcctattt aattttgact attctattat atacaaaaaa tgaaatagaa agacaaaaaa
    43981 gttgaaaaag atcgtaaata attgaacttc tttttcacaa aatggtaaga atggagaatt
    44041 tcacgcattt gctctcaact attctttctt aagcctcaaa tccgttttta tctgatacgt
    44101 ctttgaacca gtgacccgat tttttgattg ttctttttcc atcttgtgct aagtctactg
    44161 aaataaagcc atatcgattc ttatatgcat tggtccatga ccagttatcc atacatgtcc
    44221 acatatgata gcctaaacaa ttggcacctt cttgaattgc ttgatggaca tatttcagat
    44281 gagattggac aaattcgatc cgataatcat cgtggatcat gccatcttct ttgataaatc
    44341 gtgcttcgtt ttctacaccc ataccgtttt cagaaataaa gcagtcgata tttccataat
    44401 gatctcgtac attgatcaaa atatcataga ttccttgttc atagatctcc catccgcgat
    44461 aaggattcat tttacgacca ggcatttcat aattgtcgaa ataatcctca ggcattggac
    44521 cgtttttagt atcgattggt gtttcttttg cttttacccg gcgtggctgg taataattga
    44581 tacctaaaag atctactgta ttttctttga tgaccgtcaa atcctctgga tggatcacag
    44641 gcatgagatc aagttctttt actatctcaa tcaattcact tggaaactcg ccttttacag
    44701 ctggatcaag gaatgaacga ttgaagaacg catctgctat ttgcgctgct ttaacatctt
    44761 cagggtttga ttcgtctctt ggatagcttg gtgtcagatt caagatgata ccaataacac
    44821 caccttgctc catttcatgg taagatttga tggctaacgc tgaggccaac gcttcatgat
    44881 agccaacttg gatcgctttt ttcatatctg actcttctgg ataatgccat cgatataaat
    44941 aacctgcttc gactggaacg attggttcat tatgagtaaa ccattttttt actttcgaac
    45001 cgaacaattc aaaacaactt ttggcatatt tttcataggc ttcaaccgtt tctcggttca
    45061 accatccgcc tttttcttgc agatacatcg gcatatcgaa atgatacaga ttcacaaagg
    45121 gttcgatacc gttttcaatc aactcatcaa aataacgatg atagaaacga acggcttctt
    45181 cgttgattgt tccagattca tcaggtatca agcggctcca ttggatagat gttcgaaatg
    45241 aattatgccc agtctgtttc atcaactgta catcctcttg atacttcgta taaacttgtg
    45301 aagttttttc aggtcctact tggtcaaaga acttttgcgg ttcttttttg taccaataat
    45361 caaaaatgtt ctctcctttt ccatcacctg aaatccttcc ttctgtttgt gggccacttg
    45421 ctgcagaacc ccaccaaaaa tttgctggaa attgatattt tttcatcaaa gcacctcttt
    45481 ttctttttta gttcgtttta gttgattcca tgtatgatat tttccatcac aattactttt
    45541 tcattttctt cctcttcttt tttgattcgt ttcaacagct gttctgcggc ttgttccccg
    45601 attttatctg ttggtggtgt aatcgtgatt ttcggaagga tgctcaaatc gtttttatcc
    45661 aaataatcat aatgactaaa cgcaatatcc ttcccgattc tgataccttc ttgattgaaa
    45721 cattcgatag caccgatcga catattataa ttgctgacaa aaatcgctgt tactcctttt
    45781 tctaataatt cttttgcccc ataatagcca ctttttctag aatagtctcc tgtaaagacc
    45841 tctaattgga tcgatggatc attttgtacc gcttcttttg ctcctgctaa acgttctctt
    45901 gctacgtagt ctgtttgagt ggcagcaata aatccgattt ttttatgtcc ttgttcacca
    45961 tgatgagcga tcacttttgc tacactttct cgatcattca ctaatattgt atcaatacct
    46021 gctggtcctt ctgggcaatt caatgcgata actggaatac tcatttttgt gagcatttca
    46081 aatccagacc actcttcttc ggataagaag acaatcagcc catcgatcac atgcttcatc
    46141 aggtattcaa ttttttgttc aattttttct ggctgatcgg caaacccgct taataataag
    46201 ctatacccat tttcttcagc tactctttcg atccctgaga caacttccga cccaaaacgg
    46261 ctggaaatcc cgtttatcaa aagtccaata gagaagcttc gattattctt caacccttta
    46321 gcaatgatgt tttctttata gtccaacgct gaaatcgctt ctgctatttt tttcatgttc
    46381 ttttccttca gttgctgacc attcaagtaa cgggaaactg ttcccactga aacaccagcc
    46441 atcttagcca cgtcacggat cgatgccata cattctcact cctgccagtt acatttgatt
    46501 tctctttctt gttgttcgat tatgatgacc gctttctttg catagtgttg cttgtcatca
    46561 atatccatat aactaaagtc ttcagtagag caacttcctc tcactattaa ttatacttaa
    46621 acaagtattt gaaccggttc atttttaata tagcattttt ttaaaagatt gcaacacttt
    46681 tttaaaacgc tttcctaaaa tggcgaattt tttgcctttt taaaatttta ttctaaaatt
    46741 tactctattt gtgggtgttt tgtgcgtttt tctcttccat ccgtttgaag ttttactttc
    46801 caatggtata atttctttat cctatcaaat gggggaagta ttttgaagaa attaatcgta
    46861 ctatttattt tgtctgcggg acttttgaca ttgactggct gtaatttgtt tggcggctct
    46921 gaccaatcct cggatgaaac aaccgattct tctgtcttaa aagcggaaaa agcagatatc
    46981 actaacagtg tatatacgct aaaagtacgt aaagatatgg atttatccgc gggacagttg
    47041 acatttacag ataaaaaaat ggaatggaca cgcacgtatc agacacaaga aactgattct
    47101 tctgaatcat ctgaagaaaa aacagtgctg acagacatca agattactac caaagatgac
    47161 gaatatatta tttcaggaaa agaaaataaa aaagaagcaa ccatcacatt taccaaaatc
    47221 ggtaattacc gtttgaagga tgaggatggc aacatctaca gtctatagtg ccgtctgttg
    47281 gatcttcttt tgtttctccc cttcttttct gctatagtaa agaagcaaga tgattgtaaa
    47341 ggagacgaaa tggatgatcg atagtcctga ttattgtaca tgtaataaca acaaaaatat
    47401 ccatacagaa tttaatgacc aaggaagctt gagcatatgc gacaactgtc acaaactagt
    47461 caaagattca tttaaaacat ttgaatctta tcctgttcaa aattatgtaa aaagtagaag
    47521 aagataaaca aatccaagca aaactcacta tggctgattt ttatgaactt caaactcagc
    47581 ttcttccttt gatcaagctt catcagtaaa acaaacagtg gatcatttca gtttacaaat
    47641 caaagagtaa agatttattt atttcaaata tttgaatata aaataaccaa acgatagaaa
    47701 ggcttattct acatcacgta gtaatttttc tttcccgtcg tttggttttt tatttttagc
    47761 ctaattcttg accttttgca tgctggttat ccgtaaaata ttttccagca ggctttcctt
    47821 ccaattcagc tagtttttct tggatctgat ggatcacctt ttttgcatct tctggatttt
    47881 ctacaaaatc gaatttatcc ccatccactt gtattttagg acatacatca aaatcttcat
    47941 accattgatt atagcgggtg ttcaacgttt cataataggt atataacgtc tcatcgcttt
    48001 ctagctgttc atagtcacgc ccacgttttt tgatacgttc tagcatcgtg tccaaagaaa
    48061 ctttgatatg gaccatcaaa tcaggccgtt tttttggagc aacatcatct aactcgttta
    48121 gcatcgtatc taataagcta tcgtactgct gtacttcgat atccgttacc cgacctaaat
    48181 cagcgtttag atggaacaac aggctatctt catagatgga tcggtctagc acattgtcat
    48241 cgttcactaa agcatttttc attgctaaaa atcgcttgtt caaaaagaag atttgcaata
    48301 aaaaagcgta ctgctcagga tttgaataaa agagcggcaa tacttcgtta tcttcgacat
    48361 tttcataaaa tggtttagaa tcaatctctt ttgcaagcat atccgtcaaa ctgctcttac
    48421 ctgcaccaat cgttcctgaa atcaaaatca ctgacataca acccttcctc ttctataagt
    48481 actcgcctac gtaagtttca acaggcaatg tttcaaaatc atattcacgt acagcttggt
    48541 ctgattttaa atacgtatgg agactttgtg taatcatcaa gttcgtaggt accgtctctt
    48601 cttgtacaat gatcatcggt tttttcaaca tagttgccac acctaattcg taagccgttc
    48661 ctggatcgat tgtttgatgc tcaaagtcaa ggatagcaac aacaaattct gcatttgtca
    48721 gttcttccat atctttttcg tatacttcct gcgcccatcc agcgctgaat agttcataat
    48781 tgctttcttg gtgatgacgt ggagagtaga aacttgtgac tgttttgttt tcctccaacg
    48841 ctttttcgat acgacttact cgatcgattt gctcctctga aaaaaatggt cctgctaaat
    48901 atatttgact cattattttg cctccttcaa taataactga tttttaatag atactgctcg
    48961 taattcattg accagttcca atggattgtt ggaaaagatt acttgatcca accatttttt
    49021 atccataaaa tcctcttttg caaaagtttc taataattgt tgcaagccgc tgtaataacc
    49081 gccaatattc aatattccga ttggcttgtc aggcgtcaga ccgattgcta tccaagaaag
    49141 aagttgtgag aattcctcca atgtacctag acctccaggg aaaacgagga cagcatctcc
    49201 cttttcaaat aataatacct ttctttcatc gatcgtttct gtaggaataa atgtagtaac
    49261 atcttttcca ggcaattcag tttcaaaaag cccattcgga tagatacctg tgacttttcc
    49321 attattttcc agtactgttt tactaatcac tcccataatc ccggagtatg aaccaccgta
    49381 aaccagttcg atttcttctt gtgccatgta ttttcctaac gtttgcgcaa taaatttgta
    49441 actttcttta ttaccaaaac gggaaccgca aaaaacagta aatttcataa aaaaacactc
    49501 cctaactcaa aagtctatct tcattctaca cgtgtttttt taaaataaaa gactagattt
    49561 tgtagttttt tagtggtata taggatagac accactatat atagtattta gaaaaaataa
    49621 tttttcagct attttataga taaaacaaaa ataaataacg tgttttcatc gatatgaaat
    49681 gacttactta tagaagatac tgggatttcg tcctatatgc caataacgtg tattatttta
    49741 aacaacaaat acttgtttct tttattaacg aaaaaatcat tttgttctcg tgttatgttt
    49801 cactgataaa tgatgtttct ctaaataaaa aagccgacga actaaacgat caaaatttga
    49861 tcatttttgt tcatcagctt atcctaaaaa tctttttgtt aacgataaat agctggattt
    49921 gtaaaaatat cacttgaatc gtcactgctg gaagaaaatt cagtgataac cgcacctgtt
    49981 gcgcctgcct ggaactagtg tttgatattg ggctcgaacg tgtactgatc tctagcatga
    50041 agaataattt ctttttttac tgtataataa ctttctttcc tttttggaat agtacaggaa
    50101 agtttttttg tttcctttcc ttcgacataa ggtataccag accttttcga caacgaaagg
    50161 ttctcatttt tccttcatcg tcatttcaag gagggtgccg atgctctgga cagtttcgat
    50221 cgctcctaaa ccaaaatctg tatattcgat tctttctaac tctgattctt tcataccatt
    50281 cctgcctcaa ggtacatcta ttgtactttt ttcttgtaat ccattctgcc tctcctaact
    50341 aattgtcaaa tcatcccaag atatgtcttc ttcctctttt tcttcttgga tgatttctgc
    50401 attcggatct actggttttt tcaataataa aatggttatt cctgtgacaa aagaaccagc
    50461 taacaatgca ccaatgaatg cccatggtct ggtcatcgta gcgactgcaa acattcccga
    50521 tgccggaata cggctatctg cgccccaaaa catgcttaat ccgccaccta ctgccccacc
    50581 gactcccgtt gctatgacat tgcgtgccaa gtcgttcaat acgataggga aacatccctc
    50641 tgtgatctga cagatcccca taggaaaagc ggtcttcaat gtatcgattt cttgacgatt
    50701 gaatatattt ttacgaatga ttttccctaa gaaataagcg atcgtcaagc cgaatggggt
    50761 taccatcgaa gctaaaatca atacagtgat tggttcatag attccttctg aaaatcgtgc
    50821 cacaccccct tatccagaat aaatggtaat ccagaaacag ccgtgtatct gcggtaataa
    50881 gcctaaccaa acgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg
    50941 cagggcagct ttttcacaac ctcttttttt atttatgttt cataatcaat atggtaagga
    51001 tgcagctccg tatggtagac cgcctccgaa tattcaatca tttccccaat ttgcgtagac
    51061 gatttccgta tccgtttgat gccgatctgt gcgtctgttt tcagaattcc ttgttcttct
    51121 tctgttaaaa atactgccga gaaatcatca gaaaatgagt caatagtata tccttttttc
    51181 gtcagcaaac gatacaaaga ttctttgcca aattcttctc tgctgaattg catcatttct
    51241 tgtggaagat aatgaacaaa ataaatataa ggctgattgt tcaaataata taagcgctta
    51301 aaacaaacag cttgctcacc gaaaaaggaa aacaaaggat cttccttctt tatatggacc
    51361 aattgcaaat ccagcgtttc tttccggact tcatatcggg agttctccaa gatttgcgta
    51421 aagcttgccg ctttggataa tcgattgtaa ggacgattgc ttagaaccgt cgtcccacga
    51481 ccacttttct ttgcgacata ttcatctgat gccagcagtt cgattgcctt tcggacagtg
    51541 attttgctga cttgaaacag ttcttctaat tcactttctg tcggtaacat cgaaccgact
    51601 ggatattttc ctgagaaaat atcttcttta attttatctg cgacttccaa gtatagtaca
    51661 cgtgtccttt tcataacgtc accctttgct ttactattac taaaagaata gcacataaaa
    51721 aataaaaaaa gaacacaaat cacatgtgag aagtgtgttc tttttcattc agctgcttta
    51781 attacaagtt tacgccattt gaggcaatta cttctttata ccaatcaaaa gattttttct
    51841 ttgatcgatt cagtgtccca tttccttcat cgtctagatc cacatagata aagccgtaac
    51901 gtttactcat ttctcctgtt gatgcactga caagatccat acatccccaa ggcgtatagc
    51961 ccatcaaatc tacaccgtct gctactgcat tagccatcgc ttcgatatga cgacgtaaat
    52021 aatcgatccg ataatcatca ataatcgtgc catcctcttc catatgatca atggctccaa
    52081 gaccgttttc aacaatgaat aatggtttct ggtaacgatt gtataattca ttcaatgcga
    52141 ttctcagccc ttctggatcg atctgccagc cccattcact tgcctcaaga aacggatttt
    52201 ttactcctcc cagcaagttc ccttctgaag ttgctgcttg tggatcagtc tcattgattg
    52261 ccgtagacat ataataacta aatccgatgt agtccactgg atattctttt agcaactgga
    52321 agtctttttc cgtttgctct aatttttccg gatcgactcc atatttttta tgggtacgcg
    52381 ctgtgtatgc agggtattct ccacgtactt ggacatccgt gcagaaaaaa ttgaattctt
    52441 ggttttggat cattgttgct gcctgattga ctggattggc atcgatgctg taagttgttg
    52501 catagataat catgcagccg atttggatat ccgaacgcaa ttcgtggccg atttttacag
    52561 ctttgctact tgcaacgaat tgattatgcc atgcttggaa aatattttgg tactctcctg
    52621 ctccattgct ttttactaat ccttgactta aagctggaaa gtgaaaggcc gaattgattt
    52681 cattgaaagt catccaatat ttgactttac tgctgtatct ctctaatact gtctgagcaa
    52741 atcgttcata gaagtcgatc agttttcgat ttttccagcc accgtattct tttgccaaat
    52801 gaagaggcat ttcgtaatga gaaatcgtga tcacaggttc aatatcatat tttaagcact
    52861 catcaatcag ctgatcataa aattctaatc ccgcttcgtt aggagtacct tcgtctccat
    52921 ttggaaaaat tcgtgtccag gcaatcgaaa aacgataaca tttgaatccc atttccgcaa
    52981 acaaggcaag atcttctttg aaacgatcgt aatgatcgat tcctcgatga ttcgggtaaa
    53041 tatatttttc ttgatcaatc gtccagtcaa attcttcact gccgataatc gcaaagcgct
    53101 gcttacctcc aggcatagca tctgcaacgg atagcccttt tccgtctcga tcataagccc
    53161 cttctaattg attggcagca gttgctccac cccataaaaa tttttctgaa aaacgtgatt
    53221 gcatcctgat ttcctcctta ttttacaatt gccagaacat cttcgtttgc aataatctct
    53281 ttttggtcta attcaagtat atcggcaaat gcatcagtat tcgtaatgac aactggagtg
    53341 attacgctat agccagctgc tctaattgct tcgatgtcaa aacgaatcaa taaatctcct
    53401 gcttggacga gatcccctta tttgactaag atctcaaagc cattcccatt taattctact
    53461 gtatccatcc cgatatgaat caaaatatcc gctccgtcag tcgttgtgat ccctacagca
    53521 tgtccagttg gaaaaatagt cgtgatctct ccgtctgcag gtgcaactag ttctcctact
    53581 tctggatcaa tagcgatacc ttttcccatc acgccagaag aaaaaacttg gtctggtact
    53641 tcactcaatg gcagtacttt acccgttaaa ggacttttta aggtatgccg tgctgaagta
    53701 atcggttctc ctgcattcgc atgtttgttt tctaattgat tgtcttgtgt ttgttcgtca
    53761 aatcctaaaa tcaatgtacc tacgaatgca agaacaaaag caattcctgt agctattact
    53821 gctactgtca catttgactc tacaccatcc accgtactaa tgaaagtcgg aatcgtcaat
    53881 aagctgacca aactgctgga gaaagctttt actccggaaa atccgataat agctccacca
    53941 atacctccag aaatacaagc ggcaatgaat ggtttcttca aaggtaatgt cacaccatac
    54001 acagttggtt cagtgattcc aaataacgaa gtcactgtag aagaaatccc taatgttcga
    54061 agtttcgtat cttttgttcg caatgctaca gctaaagcag ccccaccttg tgccaagatg
    54121 gctggaagaa gcattggcat caatacatca aacccatatt gttcgatatt caaaaacatg
    54181 atcggtacga atccccaatg catgccgaac atcacgaata cttgccataa acttcccatg
    54241 attagacctg ctacgattgg actaaatcca taaatcgagt tgaagagacc gcctaaagca
    54301 ttaccggcaa ccgtcccgat tggtcctaaa gcaagaaatg ttaatggaac catcactact
    54361 aaaacgacaa gcggaactaa aatcatttgc agaaattgcg gaatcacttt ttttacgaat
    54421 ggctctaatt tactttgaac ccatacggct agaatgattg gtatgacaga agaagtataa
    54481 ccagatggtg aaagaatcac tgggatccca aagaaactga tagcttctcc cgcaccagca
    54541 actgctgtga tagctggata cacaagtgcc atagcaattg ctactgctaa aaactggttg
    54601 gctttgaact ttttagctgc tgtaaaagca agcatgacag gtaagaaggt aaagacacca
    54661 tctgctgcag caaacaatat ttgataagta ccgctatcag ctgtcagcca gcccatcacc
    54721 gttgccaatg ataagaatcc tttcaatacc ccggcggctg ccatcgcccc tagaaaaggt
    54781 gtaaatatac tagagataat atcgatcaga cgattcaaaa tatttttgga ctcttctttt
    54841 ccatcctccg aaactgcaag accttcctca tccacgatcg attgataaac atctgctaca
    54901 ttactgccaa tcactacttg atactgaccg ccactttgta cgacttggat cacgtccggg
    54961 tcttctttta acttttgcgt atctgctaaa gattcatctt ttaatctgaa tcgcaaccgc
    55021 gtagcacaat gtactaaact tttgacattc ttttgtcctc ctaccgcttc ccatacacga
    55081 cgaccaactg cttgattatc cattgttttt tccttctttc ttttttattt aaaaaataaa
    55141 aacccagatt gaaaaaaaca gtcacaaaca ttcgtgaacc tattttttca atccaggttt
    55201 tgcctgccat acagtaacaa tccttatgat tggatgcttt ttttctcact tactactctt
    55261 tgaatatgaa tcgttaaata aatctgctct gcgatcccca tttcctgttt ttgcgtttgt
    55321 cgaagatact cattgatccg ttcgacacaa tgaaacgctt ttggatactt tccttgaact
    55381 aatgcgtaga gaaattcatc ttgctcgtca gatccttccc ctttgagata acgctgggca
    55441 aaaaactgca gatgagtaat gatacgctga taattcaaag attcttcatc gaacacgatg
    55501 ccgaagaatt tactgatgat actcagtata tctcttacca ttgtggtagc ggtcatcgtc
    55561 acttgcatgt cttcgtttcc ttgttggcta ttcacaaaat gaaaggcaat aaaaccggct
    55621 tcatcctcag aaagttccac acccgtcttt tgtttcacga acaaaagagc ctctttcgct
    55681 gcttgatatt ctttcggata aaattttttt gtttcaaata ataatggatt aggcagttgg
    55741 atattttctt ttgctcgtgt gatcgcataa tgaagatgat cggtcaatgt cagataaata
    55801 ttaggcgaaa attcaacttt tagtttatct tcagccagtt cgactacttg tttgacccat
    55861 tcgatctcct ctgccggaat atcagccatc agctgctgga attggttttt agcaaaagaa
    55921 tctcttaaaa taaattcttt ttggaccgct tctggcgaaa tctcgtcccc aatctttttt
    55981 ttgaaaagct agtccttttc ccatacaaac gacttcttcc ccgaatccat ttttcgaaat
    56041 gacgacattg ttattcaaaa ttttatcaat tttcattttg cttcgccctc tcaagaaaaa
    56101 taaaaaaacc tagaattcgc ctactgtttc ttagctgaat tctggttttg cctgccgtac
    56161 agtaacaatc catttcttat tcaaaatcat cataccatcg aatttttatt tatgcaagcc
    56221 ttttcatata aaaaataaaa aaactggaac acaagtctat ctagaaaaca accaaatcag
    56281 agatgcacct ctttgcgttc ctcttttttt cagcaaattg gtttttcctt atcgattcat
    56341 atcctttggc tgtttttaat ctgtaacgac ttatgtccca gtatcctaaa atgtttttgt
    56401 ctctagattg aaagcagatc tcatatcatc tgtcgcatct tttatcggtg taacactttc
    56461 cagttctcct tgtacaacta gtcgcgtgac ggcgtccatg tcgccacaag taattagtgt
    56521 gacaatttct ttttcctcga cttcattcag taattgggta tctgtaggag caactttttc
    56581 tttaaaaaat gttttgtatg tatacacatt ttctaaatct gtcaaataga tcttggcacc
    56641 catctctaaa ttttccagcg gtgtaaacaa caattcaggg ttcgttgcac gatggcttgc
    56701 taaggcataa tttccttttc ccatttcttg atctggagat aatgttcctg ctccgtaaag
    56761 tagtgacgta ttatccaagc ctttgaaaat cggtaaattg attgaaactg aagggactgc
    56821 gatactagca atcactggta gatctgttcc tctcatctgc gacctcatga ccccttctgt
    56881 cgtcattggt tctaccgcat caaagtcaaa actgacatct ttatcgttat tctttttcag
    56941 atcttctttt gttacattcg ctatagcata tttatctcct gtctctctga caagataatc
    57001 cttgatctgt tcattgaaaa tcaaagctag tcctactaat aacaagagga acaagaggat
    57061 attgactagc catctttttg cttttccttt tttttcattt tctctccctc cagactcatt
    57121 ttactgtttt tcttttgctc tttccaacag ccgaatgaca catttctaaa actctgtgta
    57181 tttttttcgt acacctgcgc ttttttctac aggatatttc aaattatttt tgatcaattt
    57241 ttcttcaatg atctcatcta gatccagctg aaggttgtct gccatcatgt atgaatagat
    57301 caatacatcc gccagttctt ctttgattcg ttctaattgt gtttctttta cttcttccgg
    57361 cgttttccat tgaaaaagtt ctaacaattc agatgcttcc aaagaaatag aaatagccaa
    57421 atctttttcg ttatgaaatt gtcgccaatt acgttcatct ctaaactggt taactttttc
    57481 catgctattc atttgcttgc cttcttccaa tcatttctta cttgattaat cataccattt
    57541 ttttacataa aaaatagatg atgcttttaa aaaaactcct gttttctttt atttaaaata
    57601 aatgtcatag aaatcagtaa tttaggtagc caccatgtgt atgcagtaat gtgacttctt
    57661 cccctgaggc tttttgtttt tcgatacgtt cttctcccca aacagacatc gctactagaa
    57721 tctctcttaa tgattttcct tcttcggtga gcgagtactc tactttaggc gggacttggt
    57781 tatagacttt tcgatggaca atgccatctt gttctaactc tcttaactgc tgggtcagca
    57841 ttttttgtgt gatcgtaggg attttcttgc ttctccacgc ctaaaataag cgatattgct
    57901 cttcccttta tctggtgtaa gcaaagcagt tagcattttg acgatcgtgg gttccgcttt
    57961 tcacttctta tctacttagt atcatgaagt atactttcgt actaatacta agtagcggaa
    58021 gtgtcaacta ctttgtttaa ctacatagca aaagaaacag aagggtgaca acggtcatca
    58081 cgcttctgtt tcttttgaat atgctgtgtt gttgtctcta tctccgtgta gactttcctc
    58141 aagcttatcc tttgacagta agtcagtagg ctgatttgct ctttctagat gccagataca
    58201 tttctacaaa aaaatagtga ttctgttttg aaaattcgat ttttagttct gcattttcgt
    58261 aaacatccaa catttttttc gcactgtata gacctaaccc gccatttttt ttagaggttt
    58321 ttcctctttt gaccatctcc gatagcgtca aattcgtcgg atttgccttg ttcgctatac
    58381 taaaacgtaa tccctctttt tcttccagca gattgatcgt aatcggactc tgatcggcta
    58441 cacgatgatc gaatgcgtta gaaataaaaa tagataaaca gcgaataaaa acaaccagat
    58501 caatcgggat tgttttcact tcgacaggaa taactacttg gatcgggatt ttctccgatt
    58561 ctgctttttc tagaaatttt aaaaagacac ccctgacagc tggggataca atatttgtta
    58621 gctgttgtat ttgcggtttt tcaaaaatag ctgcagaatg atcttctaag atttctaaaa
    58681 attccaacgt ttctttttta ttttcctgaa gagtaatttt taatgaaaac agtagacttt
    58741 tataatcatg tttgaattcc tctagatgat ggtaatattc ttcatttatt tgatacagtt
    58801 gaagcgattc ctgatgttgt ctttctttta cttggaaact gatcaacata atggaaaaaa
    58861 acaaagaaaa aaaactgatg actatcgaca aaagagatag aagcagataa ttagatgagc
    58921 ggaagtgaac acttcccaat accaagtaaa tcacgcccca gcttaataaa atacaaatac
    58981 ctccaaaata gtgttcagtt ggataagtaa aaagcaagtt gagaaatctc gtctgtttgt
    59041 ctatactttt tactaataga tagaaaataa atgcgataag tagtgcaaaa aaacttgctg
    59101 ttccaagatg tctctttcct aaataatcca tcagatcaaa aaccagaaaa aagctgagtc
    59161 cagtgatagc aaaaaaaaga ttactgatca aacagctgat aaacaggttt ttcgtagtaa
    59221 aataaaaaag aaacatcaat aataagccaa ctaggtaagg aaagctcact ttagggatag
    59281 gaaataaaag tgtcaccacc atcaaagcta taaaaccaaa gctgtttttt actagcagtt
    59341 gtttcgaaaa gaataaatag atacctaaat ctaaaatcaa agtaaaggtc aaataaagtg
    59401 tcactgttgc gaccatctta ttcctccatt gttttgtttc aaaatgtaca catatttttt
    59461 taataggagc ctgcatatga aaaagtattt tatcatagaa gataaccagt ttcacagcga
    59521 atggatcatc aaagaattgg tcaatcatca tatcgagaat atcgtgcagc tcacttctat
    59581 ctctcaaatc aatcagttga tccagttggg tactattgat aatcacgatg ttttctttat
    59641 cgatatcgac ttgaagcaat cactcacagg tattgatgtc gccgaacaaa tacgtaaaaa
    59701 aaatgcacat agtaatattg tttttttcac cgcttatacg aattttgcag tagagatcat
    59761 caatcggaaa atccttccga ttggatatat cagaaaagac aggaatttat ctgaacaaat
    59821 tcgtcaagtg ctagaacaga tcaattggaa agaacagcaa atcgtaaatg tcgataaaat
    59881 ctttattgat caaaaaggtg gaagtctcgt cctgtatcct cgggaaattt tatatattga
    59941 aagtatcaaa ggtttgaaaa accaagttat ggtcaaaaca actacagaag ataaattagt
    60001 gaatacttct ttaaaacaat tgaagacaga gttggaaaag tatgcttact ttttactatt
    60061 aaaatcttac tgtatcaact tatcgcaatt gcagaaaatt gattctgctc attatgtgtt
    60121 agaatttttc aatggtgact ccttactcgt tggtcgaaag atctttgaaa agacaaagga
    60181 acgtttccat gacttccaaa aaactgcttc gagttgatga ataagagctg agacgtaagc
    60241 atagcagatt tcagggagtt ggccttgaat cggctccact ccctgaagtc attgatttga
    60301 ttttacgctt tcccttctgt aggagcatct gttaacgacc atgtgaatgt tgaaacgtag
    60361 ttgtctacgg cataattgga tacaggaaca aataattcca ctgattgtct cccttgttcc
    60421 acattctctc ctaatgaata aatccatgtg ccaatccctt catttgctcc tgcagttact
    60481 agatctactg cctgatctgc tgggatctca attccttcag cgccagtacc agaaatagta
    60541 gatggcgttc cactgctatt agttgcgcta tttcgaatca cggcgttttt gaatttcacc
    60601 tttgaaccag ttagtttatt cgtatcagat gttgtaccaa taaattctgt tcgtttgact
    60661 gatagtttcc aaccggcata aattccgctt ttatccgtta cttgtacgaa attctgtact
    60721 tgtgttgctg tccctgtact gtccgttccg ttttgtaatg cagcatatac tgtttttgct
    60781 gtacctaact tgattgttcc aaaatcaaga tctggagctt ggtcaatcgt cagtaatccg
    60841 gatgttccct gatttggttc gttaggtgta aacggcgtat cgggatcatt tggatcaaca
    60901 ggaggaactg gaacaacgtc ttcaggattt gcggcatctg gttttgttac agaaatcgtt
    60961 ccttctgtag tggctgagct cttattctca gcaaaaacac tttgtgtaca taataataga
    61021 cttccagcta gaacggtact taatgcaatt gtttttttca tttctttttc cttctttctt
    61081 ttaaaataaa taatccagca aataagccaa taagaatgac tccaacaagt atcagccatc
    61141 gccagtcaaa tgtttctggc tgatttttta aatcaatatc cttttcattc aattggtccg
    61201 ctgcatcatc agttatttca aaatcggtat ggagattcca gcgtttttct ttccaagcac
    61261 ccttcacaac cgcttgataa tgtcccggag ctaatttttt tccttctaat gggacaagta
    61321 aatcaaaatt tgtattgggt gctacatcta ttttttcttt ttttattgaa agataaggct
    61381 cttttgtttc ttttttgtag atttcaaccg atgtagtcaa gccgaacata taagaagcat
    61441 ttggattttg aagattcgcc ttaatcacat ttcttccatc gctttgatcc gcataagttt
    61501 tttttaattt caaatttggg gaagactctt tttcgctatt tcgaatcaag attgctttca
    61561 cttgagaaaa ctgattgacg acaccaactg tacttttgtt agaaacgttc tctttttttt
    61621 cttgaaacgt gattcctcct gcgaccacac cagccagctc tttttctgga gctttcagtg
    61681 aaagtgtctc agtgaccgca ctttgaggag ggacagttat ttctcccact gtttttgcta
    61741 agtttttcat atcataactt aatgtttcat caaatttact ttttgattct ccatattcga
    61801 tcacaccgtt tgaattcgtc gtagcggagt ggatcaatgt ctcaactgtt actgatttat
    61861 ctgttcggtt aaagaccgtg taggggattt cctgtgtttt cccaggctcc atccttacat
    61921 ttatatatgt tttgttctta tcaatctgat tagccggtaa ttcaaaatct accgcaaaat
    61981 tgaattcgga ggcatggaca ggtgtctgcc aaaggatgaa tccaaggaga aggatcagaa
    62041 gtaaagagca gttgtttcga tgacttttat tccatattcg attcatcaat cattctcctt
    62101 gatctttatt ctgtcggcgc atctgctaat gtccatgtca tctctgctga gtaattacca
    62161 gctatctgat tttctttggt taagacaagt ttaaacgccc gaccattatt taaatattct
    62221 gaataatctg tttgcacatt cgttgaattt tgtcctctga caatttccag ttcttcttca
    62281 ttcactaatt gatactcctc agctttcttg aaatatagga catcctgcat agaaaatcct
    62341 gcatcggttg tgaaaggctg agataatttt atcgttaaga tccatggact ttttgtttct
    62401 cgctgatcat aaatccataa tttttcctgt atatccattt ggccaaatgc ttcttgcgtc
    62461 aacccagttg ctttgatctg accacaatcg agtttggaag gggcaagaaa tgagagacca
    62521 ccttctacta caatcttcgt agcaggtgca aaaggcgggg catcatgata aacaactggc
    62581 gtatcacttg gattgtgatt cccaatttca tcgttggtac ttggcttgtt ctgcacaccc
    62641 ttagcagcag cttctcctgc ttggtcgttt aatacaattt ctaatacatc tcctgcaaaa
    62701 agttgatttt ctg
//
LOCUS       DACCCR010000008        54254 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCCR010000008 DACCCR010000000
VERSION     DACCCR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.008"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IVK93_000488"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IVK93_000488"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6385804.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IVK93_000489"
     CDS             complement(937..1518)
                     /locus_tag="IVK93_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6385805.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IVK93_000490"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IVK93_000490"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP6385806.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IVK93_000491"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IVK93_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP6385807.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IVK93_000492"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IVK93_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP6385808.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IVK93_000493"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IVK93_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP6385809.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IVK93_000494"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IVK93_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6385810.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IVK93_000495"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IVK93_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP6385811.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IVK93_000496"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IVK93_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP6385812.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IVK93_000497"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IVK93_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP6385813.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IVK93_000498"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IVK93_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP6385814.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IVK93_000499"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IVK93_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP6385815.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IVK93_000500"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IVK93_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP6385816.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IVK93_000501"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IVK93_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP6385817.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IVK93_000502"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IVK93_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP6385818.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IVK93_000503"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IVK93_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP6385819.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IVK93_000504"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IVK93_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP6385820.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IVK93_000505"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IVK93_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP6385821.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IVK93_000506"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IVK93_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP6385822.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IVK93_000507"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IVK93_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP6385823.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IVK93_000508"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IVK93_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP6385824.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IVK93_000509"
     CDS             13972..14157
                     /locus_tag="IVK93_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP6385825.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IVK93_000510"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IVK93_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP6385826.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IVK93_000511"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IVK93_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP6385827.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IVK93_000512"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IVK93_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP6385828.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IVK93_000513"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IVK93_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP6385829.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IVK93_000514"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IVK93_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP6385830.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IVK93_000515"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IVK93_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP6385831.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IVK93_000516"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IVK93_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP6385832.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IVK93_000517"
     CDS             18232..18879
                     /locus_tag="IVK93_000517"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP6385833.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IVK93_000518"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IVK93_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP6385834.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IVK93_000519"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IVK93_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP6385835.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IVK93_000520"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IVK93_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP6385836.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IVK93_000521"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IVK93_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP6385837.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IVK93_000522"
     CDS             20317..21255
                     /locus_tag="IVK93_000522"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP6385838.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IVK93_000523"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IVK93_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP6385839.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IVK93_000524"
     CDS             21935..22774
                     /locus_tag="IVK93_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6385840.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IVK93_000525"
     CDS             22750..23619
                     /locus_tag="IVK93_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6385841.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IVK93_000526"
     CDS             23609..24406
                     /locus_tag="IVK93_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP6385842.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IVK93_000527"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IVK93_000527"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6385843.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="IVK93_000528"
     CDS             complement(25688..28357)
                     /locus_tag="IVK93_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP6385844.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="IVK93_000529"
     CDS             complement(28595..28783)
                     /locus_tag="IVK93_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP6385845.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="IVK93_000530"
     CDS             29053..29415
                     /locus_tag="IVK93_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6385846.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="IVK93_000531"
     CDS             29467..31149
                     /locus_tag="IVK93_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP6385847.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="IVK93_000532"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="IVK93_000532"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP6385848.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="IVK93_000533"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="IVK93_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP6385849.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="IVK93_000534"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="IVK93_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6385850.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="IVK93_000535"
     CDS             34984..35988
                     /locus_tag="IVK93_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6385851.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="IVK93_000536"
     CDS             35989..36933
                     /locus_tag="IVK93_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6385852.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /locus_tag="IVK93_000537"
     CDS             36933..37895
                     /locus_tag="IVK93_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6385853.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="IVK93_000538"
     CDS             37922..38836
                     /locus_tag="IVK93_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6385854.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="IVK93_000539"
     CDS             38862..40643
                     /locus_tag="IVK93_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6385855.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /locus_tag="IVK93_000540"
     CDS             40991..41677
                     /locus_tag="IVK93_000540"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6385856.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="IVK93_000541"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="IVK93_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP6385857.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="IVK93_000542"
     CDS             45287..46096
                     /locus_tag="IVK93_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6385858.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="IVK93_000543"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="IVK93_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP6385859.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="IVK93_000544"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="IVK93_000544"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP6385860.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="IVK93_000545"
     CDS             complement(49648..50100)
                     /locus_tag="IVK93_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP6385861.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="IVK93_000546"
     CDS             50250..51200
                     /locus_tag="IVK93_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6385862.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="IVK93_000547"
     CDS             51193..52152
                     /locus_tag="IVK93_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6385863.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="IVK93_000548"
     CDS             52149..52904
                     /locus_tag="IVK93_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6385864.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="IVK93_000549"
     CDS             52927..53880
                     /locus_tag="IVK93_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6385865.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        17802 a         9832 c        11686 g        14934 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcggaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    54241 ttctcggcaa taag
//
LOCUS       DACCCR010000009        53370 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCCR010000009 DACCCR010000000
VERSION     DACCCR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.009"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(437..610)
                     /locus_tag="IVK93_000550"
     CDS             complement(437..610)
                     /locus_tag="IVK93_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAP6385866.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(953..1642)
                     /locus_tag="IVK93_000551"
     CDS             complement(953..1642)
                     /locus_tag="IVK93_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6385867.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1705..2469)
                     /locus_tag="IVK93_000552"
     CDS             complement(1705..2469)
                     /locus_tag="IVK93_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP6385868.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(2497..3195)
                     /locus_tag="IVK93_000553"
     CDS             complement(2497..3195)
                     /locus_tag="IVK93_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP6385869.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(3207..3986)
                     /locus_tag="IVK93_000554"
     CDS             complement(3207..3986)
                     /locus_tag="IVK93_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP6385870.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4002..4946)
                     /locus_tag="IVK93_000555"
     CDS             complement(4002..4946)
                     /locus_tag="IVK93_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6385871.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4992..5771)
                     /locus_tag="IVK93_000556"
     CDS             complement(4992..5771)
                     /locus_tag="IVK93_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385872.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
     gene            complement(6156..8231)
                     /locus_tag="IVK93_000557"
     CDS             complement(6156..8231)
                     /locus_tag="IVK93_000557"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP6385873.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(8233..9150)
                     /gene="glyQ"
                     /locus_tag="IVK93_000558"
     CDS             complement(8233..9150)
                     /gene="glyQ"
                     /locus_tag="IVK93_000558"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP6385874.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(9536..10348)
                     /gene="recO"
                     /locus_tag="IVK93_000559"
     CDS             complement(9536..10348)
                     /gene="recO"
                     /locus_tag="IVK93_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP6385875.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(10491..11390)
                     /locus_tag="IVK93_000560"
     CDS             complement(10491..11390)
                     /locus_tag="IVK93_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP6385876.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(11405..11806)
                     /locus_tag="IVK93_000561"
     CDS             complement(11405..11806)
                     /locus_tag="IVK93_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP6385877.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(11784..12260)
                     /gene="ybeY"
                     /locus_tag="IVK93_000562"
     CDS             complement(11784..12260)
                     /gene="ybeY"
                     /locus_tag="IVK93_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP6385878.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(12289..14481)
                     /locus_tag="IVK93_000563"
     CDS             complement(12289..14481)
                     /locus_tag="IVK93_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP6385879.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(14505..15476)
                     /locus_tag="IVK93_000564"
     CDS             complement(14505..15476)
                     /locus_tag="IVK93_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP6385880.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            16182..17561
                     /gene="brnQ"
                     /locus_tag="IVK93_000565"
     CDS             16182..17561
                     /gene="brnQ"
                     /locus_tag="IVK93_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6385881.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(17717..18163)
                     /locus_tag="IVK93_000566"
     CDS             complement(17717..18163)
                     /locus_tag="IVK93_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP6385882.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(18190..18366)
                     /locus_tag="IVK93_000567"
     CDS             complement(18190..18366)
                     /locus_tag="IVK93_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP6385883.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(18528..18956)
                     /locus_tag="IVK93_000568"
     CDS             complement(18528..18956)
                     /locus_tag="IVK93_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6385884.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            19048..19908
                     /locus_tag="IVK93_000569"
     CDS             19048..19908
                     /locus_tag="IVK93_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP6385885.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(20003..20296)
                     /locus_tag="IVK93_000570"
     CDS             complement(20003..20296)
                     /locus_tag="IVK93_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP6385886.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(20369..22096)
                     /locus_tag="IVK93_000571"
     CDS             complement(20369..22096)
                     /locus_tag="IVK93_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP6385887.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(22310..23236)
                     /gene="trxB"
                     /locus_tag="IVK93_000572"
     CDS             complement(22310..23236)
                     /gene="trxB"
                     /locus_tag="IVK93_000572"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP6385888.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(23381..24814)
                     /gene="ascB"
                     /locus_tag="IVK93_000573"
     CDS             complement(23381..24814)
                     /gene="ascB"
                     /locus_tag="IVK93_000573"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6385889.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(24814..26217)
                     /locus_tag="IVK93_000574"
     CDS             complement(24814..26217)
                     /locus_tag="IVK93_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6385890.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            26447..27349
                     /locus_tag="IVK93_000575"
     CDS             26447..27349
                     /locus_tag="IVK93_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6385891.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(27390..29276)
                     /locus_tag="IVK93_000576"
     CDS             complement(27390..29276)
                     /locus_tag="IVK93_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6385892.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(29372..30223)
                     /locus_tag="IVK93_000577"
     CDS             complement(29372..30223)
                     /locus_tag="IVK93_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6385893.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(30680..32464)
                     /locus_tag="IVK93_000578"
     CDS             complement(30680..32464)
                     /locus_tag="IVK93_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6385894.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(32464..34215)
                     /locus_tag="IVK93_000579"
     CDS             complement(32464..34215)
                     /locus_tag="IVK93_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6385895.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(34357..34581)
                     /locus_tag="IVK93_000580"
     CDS             complement(34357..34581)
                     /locus_tag="IVK93_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP6385896.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            34832..36418
                     /locus_tag="IVK93_000581"
     CDS             34832..36418
                     /locus_tag="IVK93_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP6385897.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            complement(36728..38503)
                     /locus_tag="IVK93_000582"
     CDS             complement(36728..38503)
                     /locus_tag="IVK93_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP6385898.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(38714..39616)
                     /locus_tag="IVK93_000583"
     CDS             complement(38714..39616)
                     /locus_tag="IVK93_000583"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP6385899.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            39895..42240
                     /locus_tag="IVK93_000584"
     CDS             39895..42240
                     /locus_tag="IVK93_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6385900.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            complement(42403..42669)
                     /locus_tag="IVK93_000585"
     CDS             complement(42403..42669)
                     /locus_tag="IVK93_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385901.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(42662..42985)
                     /locus_tag="IVK93_000586"
     CDS             complement(42662..42985)
                     /locus_tag="IVK93_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385902.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(42997..43203)
                     /locus_tag="IVK93_000587"
     CDS             complement(42997..43203)
                     /locus_tag="IVK93_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385903.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            complement(43200..44255)
                     /locus_tag="IVK93_000588"
     CDS             complement(43200..44255)
                     /locus_tag="IVK93_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP6385904.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            complement(44255..44650)
                     /locus_tag="IVK93_000589"
     CDS             complement(44255..44650)
                     /locus_tag="IVK93_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385905.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            complement(44693..44947)
                     /locus_tag="IVK93_000590"
     CDS             complement(44693..44947)
                     /locus_tag="IVK93_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385906.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            complement(44944..45339)
                     /locus_tag="IVK93_000591"
     CDS             complement(44944..45339)
                     /locus_tag="IVK93_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385907.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            complement(45693..46694)
                     /gene="ccpA"
                     /locus_tag="IVK93_000592"
     CDS             complement(45693..46694)
                     /gene="ccpA"
                     /locus_tag="IVK93_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP6385908.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            46904..48007
                     /locus_tag="IVK93_000593"
     CDS             46904..48007
                     /locus_tag="IVK93_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6385909.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(48069..48671)
                     /locus_tag="IVK93_000594"
     CDS             complement(48069..48671)
                     /locus_tag="IVK93_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6385910.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(48674..49114)
                     /locus_tag="IVK93_000595"
     CDS             complement(48674..49114)
                     /locus_tag="IVK93_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP6385911.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(49240..50178)
                     /gene="galU"
                     /locus_tag="IVK93_000596"
     CDS             complement(49240..50178)
                     /gene="galU"
                     /locus_tag="IVK93_000596"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6385912.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(50193..51218)
                     /locus_tag="IVK93_000597"
     CDS             complement(50193..51218)
                     /locus_tag="IVK93_000597"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6385913.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(51262..52092)
                     /locus_tag="IVK93_000598"
     CDS             complement(51262..52092)
                     /locus_tag="IVK93_000598"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP6385914.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(52107..53045)
                     /locus_tag="IVK93_000599"
     CDS             complement(52107..53045)
                     /locus_tag="IVK93_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP6385915.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            complement(53182..>53370)
                     /locus_tag="IVK93_000600"
     CDS             complement(53182..>53370)
                     /locus_tag="IVK93_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6385916.1"
                     /translation="NSNGILRRNGLPKSMDFREVNQTFISSVSNQRNYIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT        14870 a        11317 c         9454 g        17729 t
ORIGIN      
        1 atcttaatta tctatccatt tcatcctacg cattcttgcg taaatacaat tcactctgtt
       61 gagctgatat aaatcattgg tggtacaaag ataaaatgat aactgtttct atacctccaa
      121 aaattccagt aaatatatct atagatagtt tcaaaaaaaa ttgtcttagt ttcataatat
      181 ttaagatagt ggtacatact ttataacaac gtacttttcc aaatattatt ttccgttatg
      241 aacatccaat atattacaaa gtttaaaagt ttagatgaac tataatcttc atttctgcac
      301 agtggataat aaattcttat agataaaaaa tagcctcttc taaagtcaca aataccttac
      361 tcccacattt taagtaaaca ctctgcagca ttctctttgt tataaaatga tagatattcc
      421 tcaattttat ctcttattat gttattatca gttgatacca ttccattaat gtttcagaat
      481 aattacaata ttttgggacc ggataactcc cttttgctcg taaccactca tatccgttag
      541 gattcactaa aagtttaccg tttatttttc gattcttttt ttgaaccgtc ccatgaacat
      601 accgattcaa caagctaaat tataaaaatt ggtgtgctgt cattattttt tggaaaattt
      661 attaatggct atcaatatac ttttgtatca tactaaatag ctcgtcagtt cctatatcgg
      721 atataccaat attaaagcat ttagatcatt atatttaaga gtggtatcat cccaaaattt
      781 cgaatatttt cttttatata agaataatga tactgaattg ccccattcac ctttcttttt
      841 ttagattttc tacaagtgat tcaaaccctc cataattaca gttattactt ttgaaacgat
      901 aatatgaaca tgtgtaatct tttttattaa caatccatca ttctatccct aactaatatg
      961 ctccattggg cttgacaata acataaaacg tttttaaaat caacctcaag tcgtacatta
     1021 aacttcgatg accgatatac tctaaatcca actcaaccat tttatggaaa ctaacattat
     1081 ttctatcact aacttgccac aaacctgaac aaccaggttt tacatacaag cgttgaagat
     1141 catatctcgt gtactcagct acttccctct ccaaaggtgg ccgtggacca attagactca
     1201 tatcgccttt taaaacattc catagctgtg gcaattcatc aatgcttttg gcacggataa
     1261 ttttacctat tttagtgaca cgcggatctt ctttcatctt gaacattgca ccttctattt
     1321 cattttgatc aagtaattct tccaattttt cttcagcatc taaacacatc gaacggaatt
     1381 tatgcatttt gaatgttttt ccatttttac caacacgtgt atggctaaaa aatattggac
     1441 ttcctggctc ttcttttctg attcgataag ctacatagaa catcaaagga gaagctaaaa
     1501 tcaaaccaat aatgcttcct acaatatcaa tcattctttt aaaaaataaa tagatatatc
     1561 gtttttcaac tttttcttta tccaaccaaa cttgattagg atgtacttca ctaatacgag
     1621 ataataaatc ttcactttcc atactttcag tccctttcac tatcttcact ctctcctcat
     1681 ttgtttttat ctacgaataa agaactaaaa taaaccaaac ttcttctttt ttacctcttc
     1741 atagattgga taactgatct catccccgtt gatcagatcc tttgcgactt gtttcatctg
     1801 tactactttt tccttaccaa agtccttgac aatctgctca tagcactctt ttaagtaaaa
     1861 ggtgcgctta gagaccccgt gtgcatcaga agcagccatc tgcacgagat tatgtttgac
     1921 cattgttttg gctgttttct ggatgtcttt gccaaataca ccaacataac ttggcgcagt
     1981 aatttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt cttctcgaaa
     2041 atgcgcgttg cgttctggat ggacgatcac tggtgtcttt cctagttcta acaactcaaa
     2101 aaaaagccat tctgcatata acggcacttc cagtgtcgga aattcgatca gaagatatgt
     2161 atcatctaaa tctgtaaata aaatttcatc tcggtggatg tcttcgatga gcgttccggt
     2221 aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct ctaattcggc
     2281 ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt ttccattatt
     2341 gtggtgtggt gtgcataaaa tatgcgtgat gccttgctga atcgcttttt cagccattgc
     2401 aatacttgct tctaagtttt ctgccccgtc atcgattcct ggtaggatat ggcaatgtag
     2461 atcgatcatt tctcttgtct cctctcttct tattccttaa tttctgtagt aataaccagc
     2521 atctttgcta tgttctgctc cgttgtatac aacacctaag actcgtgctt gtaccatctc
     2581 tagcagttct tttgctttag tcaaggattc tttacgtgat gtattttcac gtacgaccaa
     2641 taaagtgcca tccgtttttg aagacatgat ctgtgcatct gttacggcta ctactggtgg
     2701 catgtcgaag atcactacat caaatagttg tcttgcttct gctaaaatct gatccattct
     2761 tggcgagctt aataactctg atggatttgg tggtttaggt ccacttggca gaatcgacaa
     2821 attctcgact ggtgttcgtt ggatctcatc tgctacactt cctgatgaac taagtgctgt
     2881 acttaatcca ctggcattgt tcagctggaa tgtctcatag acgacgggtt tacgtaaatc
     2941 tgcatcgact agtagaactc gttgaccgga tttagcaaaa acgactgcaa tgtttgctgc
     3001 aatggtggat tttccttctc caggacctga tgaagtcacg acaatcgttt tgatttgctg
     3061 accagctgcg gaggcaaact ggatattcgt ccggatcgta cgatattgtt cggcgattgg
     3121 tgaagatggg tttgttaatg tgatcaaact gactacatga gtttgtgctg ctttttgttt
     3181 ctgtgttcgt gccatttttc ttcctcctag actcttgagc ggcttctgcg tgatggagct
     3241 gttttatcgt ttgtagcttt tggtcgtgca ggagctgatc cttttttacg gataccagcg
     3301 ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc taatgtatcc
     3361 gtcacatatt tttcatcttt cactgtccga tcaagtaatt cgaataagaa tgccaaacct
     3421 acgcctacca tcattcctaa tactaagcca atcactagat tcaatttatt atttggcgaa
     3481 acaggtgtcg tattggcaac tgcacctgag atgatcgaga ttttatcgac attcatcacg
     3541 tcttttgcgt tctcttggaa aacttgtgaa gttgtattag cgatctgttg agcagtcact
     3601 gcattcgtgc ttattgcctg gatcgagaac atttgtgagt tttctgactg attgacggag
     3661 atcgcatctt tgatttgtcc agcggtcatt ttcacattgt cttctgtttc tagacgatct
     3721 ttgacttcat ttaatactag atcactaacg atcatatctt tatacgtatt gatcatttgc
     3781 aggttcgtat tgacatcatt gacattcgct gtgtctgatt gcggcagtgt cacgatcaat
     3841 tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc
     3901 aagccagcaa acattgagat gatgattgtt gccaagtgtt tgcgtaatgt tccaaaaatt
     3961 tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc
     4021 atttgatctt ttaattcttt tttcacacga tcaagttctt gatcgctgac tttttgatag
     4081 gagatattat cttgcatgaa gccttctcct tgcagctgat cttgcttgat cgtaccaaat
     4141 gcctcccgat aatctaaagc gatttttttc atttcatcaa agctcatgtc tgttttcata
     4201 ttcgattcta gagcattcaa aacggattga tagttgctga caccgtttag actcaatact
     4261 tttttcgcta caccttctac gatctttctt tgacgctctt gtctaccata gtcaccattt
     4321 ggatcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa tgtgatttta
     4381 ccgattgtaa agtcatagcc atcttgtgag aaagtgaggt cattattgac ttcgatacca
     4441 ccaactgcat caacaagttc ttttagccct ttcatattga tagagacata gtgatcgatt
     4501 gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa
     4561 gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt atctcgagga
     4621 atgctgacga tcgttgtctg tttgtcttca ggactgactg tggcaaccat catcgtatct
     4681 gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa tagaacagag
     4741 aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt tcgttccact
     4801 gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc tcctactcct
     4861 acaattgcta atactaatac gataagaact gctaaaataa tcagaaatat ttttttccct
     4921 cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg cttgaatcat
     4981 cgctttaact gtcaatagtt tatattgcta tagcccattt gctgagcaac agtttgtgct
     5041 tttgttgcgt atgctacgct tattttttca ttccggatct gctctatttg actgagcgcc
     5101 gtcaaatatt gatcgtaagt agcggtggca gtcacttgac catcctttaa catcgtatcg
     5161 aataactgat ctagttgcgt gacttgcgcg atctgggtat cagcgaatcc tagatattgc
     5221 ataacaaccg ttttccaagg actatcttta aaaaagctca tattttcacg tacacgagcg
     5281 acatctgcac ttgtcaccct ttctttgatg atgacatcgt ctacagcttg ctgccagttt
     5341 gatacatttt tagtaaataa atcgttgaca gctgtctgga tcttcagttt tgattctgca
     5401 ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc ttctttaggc
     5461 aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacagagga tagcttattt
     5521 gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt ttcttttgta
     5581 ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg attggtgatg
     5641 gcagattctg cataattgat ccgttgctga tgttgccgct gattattgat atggtatcca
     5701 aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga cttgacgaat
     5761 ggcttttcca tagattcgct cctaagtatt gatgatagat aaattaagat ataattcact
     5821 tacataaaaa tgacagtttt ttatttaaaa aaacataatt aaactaatat attcacagaa
     5881 atatttgtaa acaagttttc cctatataat cataccacaa actgctttct tgactaggga
     5941 aatttacgat ttaaaagtaa ataaaacatt aataagataa caattaatcg attttacttt
     6001 tatttcctta attttcatag gtgtttttaa aagttacaca aaaaataaaa taacaaaaaa
     6061 aattaacatt gaattaataa tagttattta taacaaataa accagagaaa tagcctattt
     6121 tgtgcctgtt ctctggttta tttacatatt agaaattatt ttgtgatgat ttgatctaaa
     6181 cttgctagtg acaaagccat ggcagcaatt tgacttaatt gcgttaatcg gttcttacgt
     6241 actgctggtt cttctgccat gaccatcgtc tgatcaaaat aattttcgat cagtgggcga
     6301 aggcttacaa gctgatcata attttcgttg atcgtttggt cagcaaagct tgttttcatt
     6361 gcctctactg ctttgtgcag ttctttctcc gcttcgtttt cgaacagttc agggtctact
     6421 tctgattgtt tttcaagctc cgcttttttc gctaagttca cgacacgtgt caaagcttcc
     6481 atcgatggtt tgaagtcttc atctgcaaga tgctgcttga acaattgtgc tgctgcaaat
     6541 acttttgtca gatctttttg ttctgcattc aaaacagctt cgatgacatc atgacgaata
     6601 ttcttcgttg tcaataattg gcgcagacgg cctttgataa agtcgatcac ctgttgttgt
     6661 cccgctgaca agccaatgcc aaaacgatcc gcatctttac tgataacttc actaatcgtt
     6721 tcttgcagaa cagataaagg aaaggcccat tttttcgatt cgatgattcg aacgacacca
     6781 tatgcttgac ggcgcaaagc atatggatcg tttgagccac ttgggatcat cccaactgca
     6841 aagaaagaaa agatactatc taatttatca gcaatcgcta aaacagaacc tactgtagtc
     6901 tcaggtagat ctccttcact ggatataggc atgtaatgtt cacggattgc tgttgctacg
     6961 gctggtttct cgcctttaag cagagcatat ttttctccca tgatcccttg tagttcaggg
     7021 aattctccta ccatgttggt tactagatca aatttataga tttcagcagc gcgttgcaga
     7081 tccatcaatt cttcctcaga tagtcctact tgctcgccta tgatgcgggc gatgacttgt
     7141 acccgttgca ttttctcgta gatgctaccg attttttcat ggtaagtaac agtttttaat
     7201 ttttcaacgt attggtcgat tgaagctttc ttgtcttcat tataaaagaa ttccgcatct
     7261 tctaaacgag cagtcaatac tttttgattg cctcgaacca cattttccaa ttttacccga
     7321 tcaccattac gagcagaaat aaaatgagga agcagcatac cttgatcatt ccgtacctcg
     7381 aaataacgtt gatgttcttt catagaagtg actagtactt cttcaggaac agaaagatat
     7441 ttttcttcgt agtcgccaga aaagacagtt ggatattcga ctaaattgtt gacttcttct
     7501 agtaaatctt cgtctagatc aataacccag tgattttttt ctgctaattc gtttgcttgt
     7561 gtaacgatca tttcttgtct tttttgcgga gacgcaatga cgaattgttc attcaatttt
     7621 tcttcatatt cttttgcatg cgcaaatgtc acatcttcgc ctaagaaacg atgacctcga
     7681 gaggctctgc ctgttttgac atccaagatt tcgaatggga ttacttcatc atctaacaat
     7741 gccacgatcc agtgaatagg gcggatatat tcaaaatcgt attctgccca atgcatcgtt
     7801 acagggaaag tcaggctttt aatgacatct gctaaacctg tcaatacttc tttggattcc
     7861 ttaccatgag tgtatttcgt tacatagaca tattccacac cttttaattc tctaaaagtg
     7921 atagcgtctg tcgtcagacc ttgtccgcgg acaaatcctt ccgctgcttt cgtccagttt
     7981 ccatctgcgt ccaaagcaat ttttttggct ggtcctttga cttcttcttc actgtccgct
     8041 tgtcgttctg gaatatttgt tactttgatt gctaaacggc gcggtgtgga aaatgtttcg
     8101 atcgtatcat aagtcaattg gttttcatct aaaaattttg ctgttttttc ttctaattgt
     8161 ttcatgcttg gtgtgacaac atgggcaggc atttcttcca gcccgatttc aagtaataag
     8221 tcttttgcca tcttattcag cctccttcac ggtttctgga tttgctgtgt ttttattcaa
     8281 caaagggaat cctaattttt cgcgttctgc cacaaagatt ttggctacag cacgagccat
     8341 gttgcggata cgggaaagat aaccggcacg ctctgttacg gatactgcac cgcgagcatc
     8401 taataaattg aatgtatggc tgcatttcaa aatataatca tacgctggat gaacaagatt
     8461 ttcatcgata cagcgtttag cttctttttc aaaacgatca aagttgctta gtagcaagtc
     8521 ttgatcgctt acttcaaaag aatatttgga atgttcatat tctggttgtt tgaagatctc
     8581 gccatatttc acgccatcag cccattctaa atcatagaca ctttcaactt cttggatata
     8641 agaagcaaga cgttctagtc cataagtgat ctcagaagtg actggtttac aaggcagtcc
     8701 accaacttgc tggaagtacg tgaattgtgt gatttccatt ccgtcaagcc atacttccca
     8761 acccacaccg gcacagccca tggatgggtt ctcccagttg tcttcaacaa aacggatgtc
     8821 gtgttctaat ggatcgattc ctaaaaggcg caagctttct aaataaagct cttggatatt
     8881 ctcaggtgaa ggtttcatca cgacttggaa ttgatgatgc tgatacaaac ggtttgggtt
     8941 ttcaccataa cgtccatcag ctggtcggcg agatggttct acgtaagcag cattccatgg
     9001 ttctggtccg attgcccgta aaaaggtata tgggctcatc gtacctgctc ctttttctgt
     9061 gtcatacgct tgcatcagca tgcagccgtt tgatgaccaa aatttttgta atgttaaaat
     9121 catatcttgg acggtcaatt ttttactcat ctgttttgcc cctttcaatt ttaaagcgtt
     9181 gctgtcaaat aagaatacag gaaagaaaac gacaaaaaat ccctatgtct gattacagac
     9241 atagggacga ataattcgcg gttccaccct acttccgaat aaagattcgg cagcttcatt
     9301 tatcgtactc gcaagtgcct cttcaaaaat cgtttgggtc cggctcccac tctcccgaac
     9361 tcgctaaacc acgtgttttt tactgatctt ggtcaatgta cgtattcatt ttgcagtttt
     9421 cttgtttcta taatagatcc tctcactgaa cgatctattt gatctacggt taataataga
     9481 cggaactccc tgtgtttgtc aaatggtttt gattttttgt tgtatgtttc atttactatt
     9541 cttctttttt aggtagatct ttttcagaca tgtctttctc tggcgtcttt aatacgtgtg
     9601 cccaagactt catttgatcg atgaactttt tgcttttcaa atgaataccg acatattcat
     9661 catacagctg gtcgatgagt tcacggattg cttgtttggt ttcttctttc agatggattc
     9721 cactgatttt atcataagaa atagctgaaa acatccgaac gaaataaaca gctcgaggat
     9781 ctgcatgata acggtgatcg tccatcgacc aatgtctttc gcatatcaca ccaccatatt
     9841 tggaagaata gtcaaatttt ccttttgttt ccccacatac gacgcagtgt gtccagattg
     9901 gtgcaattcc gaagcgttgc agtatttgaa cttcaaagat attcgtgatg atttctgcat
     9961 catctccttg atccatccgc tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat
    10021 attgatgatc ttcgattgct gcatctacta gatttagcaa ataggtacca taaccgctca
    10081 aaaagatatc ttcttggatg ttccgaaacg catggacgtc tttgacactg cttaaaaacg
    10141 acaaaccttc acttcgaaaa ttaccgatat agactgcttc ggtaaagggg agcaaagccg
    10201 gagctaatgg ctgattttgc cgatggattc ctttgatata aaacatcatt ttgccagaag
    10261 attccgtaaa gattttgact aatttatctt tttctttgta attacggcta gataaaatca
    10321 agcctttcga ttctgcaagc tggctcatat tctccctcct aaaaaataaa tctggaaaag
    10381 cagattctgc cgatccagat tatttatgtt tcgatcttcc gccgtttttc tcaacgtgcg
    10441 gatttttatc cagtttattt ctatacttaa cctataagaa gttcagaata tcagtattca
    10501 tcttttcggt agccgaagtc ttgcagatag acttttttgt ctcgccagtc tttttggact
    10561 ttgacccaaa gttctagaaa tactttgtct cctaaaagat tttcgatatc ctttcttgct
    10621 ttcgttccta cttgcttcag catttttccg cctttgccga taatgattcc tttttggcta
    10681 tctctttcga cgatgatcgt ggcctggata tggattttat cattctcgtt tcgcttcatc
    10741 gaatcaacaa cgactgcgac agaatgaggc acttcgtctc gtgttaaaaa caagactttc
    10801 tcacggatca attcagaaac gatgaagtat tcaggatgat cagtcacttg atcatctgga
    10861 aaatattgtg ggccctctgg catttcactg actaatacat ccatcaaacg ttcgacgttg
    10921 tttccttctg tagcagaaat tggaatcact tgtgcaaagg gcatctgcgt tgaataatct
    10981 tcgatgattc ccaacaactc gtctggatgg attttatcga ttttattgat aatcagataa
    11041 acaggagaat gcacgttttt tagacgttca ataataaaat cgtctccccg gccgcgtttt
    11101 tgatcagcgc tgatcataaa taagaccgcg tccacttctt tcaacgcact gtaagctgct
    11161 tctaccataa aatctcctaa acgatgcttt ggtttatgga ttcctggtgt gtcaataaaa
    11221 atcaactgag cttctggtgt agtatatact ccttggattt tatttctagt tgtctgcgcc
    11281 ttatcactca tgatggcaat tttttgaccc acgattcgat tcaataatgt ggattttcct
    11341 acattcgggc ggccgacgat tgcgacaaac cctgatttat gttctggcat atacgttctc
    11401 ctttttaatt aaacaacaaa tgatatattt tagggacaaa taaaatcaac ccaacgatga
    11461 cggcaaaaca agcggtcact aaaactgctc cagctgccat atctttgatt tttttcccaa
    11521 tcggatgaaa atgaaaatcc gtaaacatgt ctacgacatt ttcaaaaaca gtattgatga
    11581 tctctactat ccatacgaga aaaacagcta aaagcagcca taaccattct atccgatcaa
    11641 gttggaaaat cacgcccata aacaaagcga ggattccgaa cgcaacatgt ttcctcatgt
    11701 tgcgttcttc ctcaaaaaca gttttgactc cttggacggc aaattccacg gacatcagga
    11761 aatgtttgtt tttctctacg tttttatctt ttaagtccat aggcatctaa gatctctttc
    11821 tgtaaaccaa acatttcttt ctcatcctct ggtgtcatat ggtcataccc gttgatatgc
    11881 aagaatccat gaaccgctaa aaagccaagt tcccgatcat acgaatggcc gtaagcttct
    11941 gcttgctctg cagcacgttc agtagagatc atgatgtctc ccagtacacg aggcaatgga
    12001 ttctctccat cttcaaaaat cactgggatt tcgtcttcac cctcttcttc caaggcaaaa
    12061 ctgatgacgt ctgtcggtgc atcttttcca cggtaatcac gattgattac ttggatcgct
    12121 gcgttgtcca tgaaagtaac agacatttct gtttcttctg gcaactctaa atatgatgct
    12181 gcaaattgca acaagtcgtc gatttctttc attttctctt ctgagacttg gtttgtttca
    12241 tccatgaagg taatatccat tatacatcgc ttctttctat tcttcttgct attcttcttg
    12301 ttcttctttt atcttctcaa cttccgattt cattttagga tatttgatcc gagaatggaa
    12361 ggtactgctt aaaccgctga tcaatgattt ttctacttta cggatttctt ttaacgtcaa
    12421 tccactgtca tccaattgtc catcggaaat acgctcttct ataagattat ggacaaaact
    12481 ttcgattttt tcgcttgttg gatggtccat tgcccgtact gccgcttcac aactgtcagc
    12541 tatattgacg attcctgctt cacggctttg aggttttggt ccaggataac ggaattgctc
    12601 ttccgtgact tccgggttgc gttcctttgc cttaacgtag aaataacgca tcagcgtggt
    12661 tccatgatgc tgtctgcaaa tatcgatcac catttgcggc atattataat cttccaatat
    12721 tttggcacca tctgtcacat gtccaaagat aatatgttta ctgtcttctg gcaataagaa
    12781 attgtgcgga ttttccgctc ctgaaggcaa gttctcaacg aaaaagctgg cgtgtttgat
    12841 cttgccgata tcatgataat agcaagctac tcgagttagt aaagaacgtc caccgatttc
    12901 tgctacagca ttagcactta gattggctac catcatgcta tgatgatacg tacctggtgc
    12961 ttcttctaac aactgtttca ataaagggtg attaggattg ctgagttcat ttaatacgat
    13021 cacactatca tcattgacca tcaattcaat ataggggtgc agacctatcg tcagcaagaa
    13081 tgagaacaat gcacctgtga atccgcagac tagcgttaac caagtcgttg catccccaaa
    13141 gctcattcct tggtagataa tcaaaatgac atccatcatc acagggaaaa taatgaccca
    13201 catagcagcg aataaccctt gttctgagat ccgtttgcgt ctgacgatcg ttgccatgag
    13261 accagaaaat acataggaca tcaaaataac tgtcagaaag ttcgtgccaa tcgaatcata
    13321 aaaaatgaaa agtgcggata ctgcttggaa aataccagcc atgatccctg ctcttcgatt
    13381 caagaaaaag cttaaaacaa gtggagcaaa agccgcgggg taaaacagcg gaatataagc
    13441 agcttgttct gtttggaaga gttggaaaaa tttcattagt actacgctga ctgacattgc
    13501 tgtcacgtag aaaagtacgt attttgttct ttctcctgca ttttgaaatt gaagtgaatt
    13561 ataaatcagt acaccgattt gaagcaaaac agctaaaacc atggcaatga gtgggaaaat
    13621 cgaagtcgtt tgattcgtca atcctagtaa ttccaatttt ttcatcgctg aagcatcaat
    13681 ttgatttcct tcgcgaacaa tgatttctcc ttggaagatc atcactggtt gtacagcttc
    13741 tcgtgcagat tgttttaatt cttctgtctt tttctcattc aatacatcat tgactacaat
    13801 gccttgatcc actaagtagc ggatcatttg ttgctgctct gttgtgacat tcaaatactg
    13861 aatctgatct tctgcttctt gtttaaaagc ttccaattcg ctttcacgaa cttgtttact
    13921 catctgttcg tcaatgagct tcaaactttc atccctaacc gtgtgaattt ccgtttcagt
    13981 catttcaaat atcgtcgaat aaaaattgtt cggcagtttt tggtaaaacg caacgttttc
    14041 tgcattcacg ttttcaaaat ttttcttcag tgtagcaatc cgctcttctg tagtaggttt
    14101 cgggatattt tcatcttcct tcgccttatc taccttgtca ttataagatt tgttgatggt
    14161 atcattcgtc ttatcgatca attcgaataa ttgcttgatc cgattgttct ggtcatctgc
    14221 cagatctttt tgataagtat attctggagt cactgcttct gctgctagtt ttcttctttg
    14281 ttcggtttct tccgtgttct cgatcgtttt attcgcacgg atactctctt ctgctacttg
    14341 gccttctcta aaatcattgg ccttttgttt gacactacta aatgtaacgc cgcaaacaat
    14401 gataaaaaac aagaacagaa ggaaaggcaa tgctgccttt ccgagttttt cttgtagttt
    14461 ctgcagtgtt ttattcaaca tgtgcttatc ctcctaagca aagcttattc ttgatgaaga
    14521 tccgcctttt cataagcttt gatgatttcg gcaactacag ggtgccgaac aacgtcactt
    14581 gcttcaaaat taacaaaatc gatttttttg atttgcttta gtgttcgttc tgcatgaaca
    14641 agaccacttg tcgtccctcg aggcaagtct atctgacttg tatccccatt gacaatcatt
    14701 tttgattgat aaccgagacg ggtcaagaac attttcatct gagcgactgt cgtgttttgt
    14761 gcttcgtcca agataacgaa tgcatcatcc aatgtccgcc cccgcatgta agctaatggt
    14821 gcgatttcga ttacgcctcg ttccatcaat cggttcgtat gatccaatcc aaaaatttgg
    14881 tataaagcat cataaacagg ccgcagataa ggatccactt tttctttcag atcccctggt
    14941 agaaagccta ggttttcccc tgcctctaca gcgggccgcg tcaaaatgat tttttgcact
    15001 tcgccttttt ttaaggcagc aattgctaat actacggcaa gaaacgtttt ccctgttccg
    15061 gctggcccta caccaaatac gatgtcatgt ttggctaccg attcaacata tttcttttga
    15121 ccgctgtttt ttacccgtat aggttttcca ttacggtctt tgacgatctc ttcctcatac
    15181 atctcaacaa aataatctaa tgtacctttt tgccccattt tcaatgccgt cacaacatcg
    15241 ggcgtactta cgtggattcc tcgtttgatc aattcctgta atgtccgaat gactgaggca
    15301 gccaaggaaa tctgggattg ttcaccaatg atctgaatga tttctccacg tgtatggatg
    15361 gttgtttccg ttgtttcctc gatcagtttt atatgcttgt cgtgtgcacc taaaagcatg
    15421 ctgacatcat cagcagctgt caatctaatc tctaaagaac tagactcttc cgtcaaaaat
    15481 agacatcccc tttttttcga ttcattcttt cctattttac catactcaga agaaactata
    15541 tagcaaataa gaaaaggttg taaaagagtc gtttaaccat acgtaccaag aaacgttaaa
    15601 aaatagccgt ttccattcgc taaaggtctc gttttgataa aaagaatctg agaatgtaag
    15661 ttatatgaaa gtggagaaca aaatattccg tttcattcag caggtattct ctaggtattt
    15721 ttcttgtttc aaaaaaagaa aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca
    15781 ggtgtattat tacgtcaaca aataaacata cagcaaagcg tcgaggaaaa cagtaggcta
    15841 ttcaacgatt tattcccagt gagtcatgta agtgagaaat gacaataaat cgaatagacg
    15901 aacatcattt ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc cattatcagg
    15961 ctagcggata actttatcaa aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa
    16021 ggtggtaccg ttctagaatc tagagccctt ttcccaagtg gaaaagggct ttttttatat
    16081 tttaaggtgg tggttttcat ggaatctgat attaatggca ttactaattg ctgcaacgtt
    16141 tatatcgtca atcaaaatga taattattgg aggaaaaatt tatggaagaa acaaaaactt
    16201 attcatggaa acaactattg atggtcagtt cacttatctt cgggatgttc ttcgggtcag
    16261 gtaatctaat ctttcctgtc cacttaggtc aaatggctgg cacaaactgg gtcagtgctg
    16321 gcgtaggatt tgccatctca ggcgctcttt tcccattact agctatctta gctgtagtag
    16381 tgacaaaaag tgatggtctg tatgacttag ctaaacctgt cggaaaatgg tatgctgcct
    16441 tgttcttaat ccttgttcat ttgacgatcg gaccattttt cggtacacca cggacagctg
    16501 ctactgcttt tgaaatggct gctaaaccat ttttgcctga aaaatataat tcattaggta
    16561 tgtttttatt ctctgctctt ttctttttac ttgcttattt ccttactgtc catcccacac
    16621 ggttggtcaa atacgtcgga aagtatctaa actccttgtt tcttgcatta ttagcaatca
    16681 tttttgtcgt ttctttattc cacccaatgg gaaatctaaa tcaatctgct gcagatactt
    16741 accaaacaaa cgctttaaca aatggtgtcc tagaaggtta taacacggtt gatgcagtag
    16801 ctttgcttgc cttaagtgtc acttttgtcc atgcagtccg tgaccttggt ttcaaagata
    16861 aaaaagtcac agaattaaca gcaaaagctg gtagcctgag cgttgcgcta gaagtcatga
    16921 tctattttgg acttgttctt ctcggtgcga tgagtttgaa taagatgtct ttatcagaaa
    16981 atggcggaac tgcactttct cagattacta atttttattt aggtaacttc ggcgctagct
    17041 tcttaggagt gatggtcaca ttaggtgtct tcactaccgc tatgggactc gttgtttctt
    17101 ttgcacaaga tttccataaa ctgttcccta aagtcagtta tatgacatgg ctgcggttaa
    17161 cgactttcgt ctcttttgtc gttgcaaatg ctggtttgga caatatcatc caatggtctt
    17221 taccagtatt gatgcttttg tacccattgt cgcttgcgtt gatcttgctt tccttaacag
    17281 caaaattttt ccaaaagaca ccatttgtct atcaagtaac gatgttgttt gctgctgttc
    17341 ccgccgtatt ggatatgctg gcaaattcgc ctgctttagt gagtcaacaa agagttgtag
    17401 caagcatgct cgaattttat catcatcacg ttccttttgc tgcattagga cttggctgga
    17461 tggtcccaac gttacttggt tacgctggca gcttactctt ctattacgcc tatcgcctat
    17521 cagggtacaa acaagaagca aatgaactac cagaagaata accagatcaa acacaattta
    17581 acataagaaa tattaaacag gaaaaaacca gctagatgaa agaatcctat tctttcgtct
    17641 agctattttt tagatttctt ttttacgagc acaaaaaaac tgcggacaat gattgtccgc
    17701 agtttaaatg ataaaattaa gattgtaata gttctttcac aatggcattt acttggtttc
    17761 catctgcttt tcctttgact ttaggcatga tcgcacccat gactttgcca aattctttca
    17821 ttgatgaagc accagtttgt gtgattgctt cttgaacaag ttgtcgaatt tcttcgtcgg
    17881 aaagttgttc cggcatgtat ttttcgacta tcacaatttc actttttact ttttcagcta
    17941 aatcgtcacg tcctgcttct tcaaactcat gcaaagaatc acgtcgctgt ttcatttcgc
    18001 gagacaacac cgttaattct tcttcttctg tcaagtcgtg accttcttta atctgctcgt
    18061 tttgtataga tgacttgatc attcggatga cttgtaaaga ttccttatct ttcgctttca
    18121 tcgctgtttt catgtcatcg tttaatctac ttagtagtga cacactacca actccaattc
    18181 atcacagaac tagaattttt tgcgttttct agctgcttca gattttttct tgcgtcttac
    18241 acttggtttt tcgtagaatt cacgtttacg agattcttgt aaagtacccg cttttgacac
    18301 ggaacgtttg aagcgacgaa gagcatcgtc aagtgattcg tttttgcgaa cgactgtttt
    18361 tgacatgtta attccctccc tccgagctta aaaagtaacc gtaattgtta ttgaaatata
    18421 tctcaaaaaa caagactgtc ctgtaaacat tatatctaat gctgttttga ccgtcaagga
    18481 aatcttgctt ttttttaaaa ttatcaaatg ttattttatt taaatcctta ttgacaattt
    18541 tcacatcttc cgaaaatctc gaatctatgc ccttcgatcg tacatccacc gagctgttct
    18601 tcgaaataat tcattggaca catatggatt tctttcgttg ttccgcacac cgtacagata
    18661 aagtgatggt gatggccgat ttccccttga cagcaatgaa aacgaaactt catctctcca
    18721 tttaaatcgg tttcttccag taaatcgatt tctgcaaaat catggagatt tcgataaatc
    18781 gtatcgtaac ttactccttt gaattcctga ttcatatagt caaaaacttc tttagcggat
    18841 acgtagcgat tgcgcttcac taaataagtt aacaatgctt ctcttttttt agtatattta
    18901 aaaccgttat ttttcaatgt ttctagtgat tgttcgatca atgtctgttt tttcatgact
    18961 tattcctcca aatcaaaatg attacgaagt acacattcta gagtatggta taataaacat
    19021 aaaaaaaatg caagtgaggt tttatccatg tcttccgatt ctacagaaat cgttactttt
    19081 tttgtcatct ccgactctgc tggagaaact gcgacaaaat tagcgcaagc cactatggcc
    19141 caatatccta gcgttgagtt caatttattc cgcagaacat ttgtgacaga tgagaaaaca
    19201 ttgatgcagg cactgcaaga tgcactagaa gaaaaagctc ttgtcttaca cacattgatc
    19261 aatcaagaat tgatcgagat gacgcgttct ttttgtgaag aacatcaatt atttagtttt
    19321 gatgtgatga ctccgccgat atcagaaatc gagcgcttaa ctggtatcaa gcccattcgg
    19381 caacctggcg cccttcattt attgaatgag aactatttca aacggattaa agcaatggaa
    19441 tttgctgtaa aatacgatga cgggaaagat cctcgtgggt ttctagaagc agatgtcgtt
    19501 ttgctaggcg tatcccgaac ttccaaaacg cctttgagct tgtttttagc taacaaaaat
    19561 ttaaaagttg ccaatcttcc tttgatccca caagcacata ttcccaaaca gctttgggag
    19621 attgatccta aaaaaatcgt gggattgacg aataacccag atattttaaa caatatccgt
    19681 aaagaacgga tgcgctctta cggattaaat cctgatactg cttattctga tatcgaaaaa
    19741 atacgagctg aactcgattt tgcaaatgat ctttatgaaa aactgggctg cgtagtaatc
    19801 aatgtcgctt ctttatctat tgaagaaaca gcttcgctta ttttgaatgc tttagaccta
    19861 gaagatcata gttattatgg aactgaaacc accgaagaaa cagactaaca gtttttcttg
    19921 tctttttctt aaaaaaaact ggactgtgtc agcgaagaag attcgttgac acagtccagt
    19981 ttgtctctat ttgcacctat cgttattgat gttccacatg tttgagtgtc tggttcaaaa
    20041 tatccagtac gtgagaatca tcaagactat acaaaatcgc ttttccctca cgatgggact
    20101 tgacgacatg gttttctctt aataaacgca gttgatgcga tacagcagat tgttccattt
    20161 ccactgcttg ggtgattgct gtcacatttt gttccccgtc tttcagatac agcagaattt
    20221 tgagacgagt ggggtcactc aacattttga atagttggct gactttctct acttttgttt
    20281 tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa agactgactt ctaaagttta
    20341 ccactttttc catcagtctc aagttttttt attcttctgt catcttttgg ataatcgctt
    20401 cataagcggc tactttttca gctgcgtctt ctgaagagtt tccttttaca ccgatgtaaa
    20461 atttgatttt cggctcagtt cctgaaggac ggatagcgat ccagccattt tcttccagcg
    20521 tatacttcaa tacatcagat ggcggtgtcg ttagtttttc tacgtttcct tcacggtctg
    20581 ttctcgtcaa ctctttgaag tcttctgttt ggatgacttt tgctccggca aattctgttg
    20641 gtgcttcttc acggaatttt tccatcaaag cagagatttt cgcagaacct tcttgtccgc
    20701 tcattgtcac agaaatcgtt ttttctgcgt agtagccata ggtttcaaaa atcgtttgca
    20761 agccgtcata caacgttttg ccttgttttt tatagtaagc tgctacttct gctaataaga
    20821 gcaatgcttg gatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa ccgtaacttt
    20881 cttcgaatcc gaacataaaa gtatgggaat gatcttcttc aaattgttga attttttcag
    20941 caatgaattt gaatcctgtc aatacattga acatggtagc cccatagtgt tccgcaattg
    21001 ctgtaggtaa ttcgctagaa acgatcgatt tcaataccgc tgcgttttca ggtaaagtgc
    21061 ctcgcgattg gtgtgcttcc aaaatatatt gagcaaacag tgctccgatt tggtttccgg
    21121 tcaatacttc ataggagcca tttggcaaac gaacggctgc acctaaacgg tctgcatcag
    21181 gatctgttgc aatcagcaaa tcagcgcctt ctttctcgcc aagtcgtatc gcatactcaa
    21241 aagcagagtg ttcttctgga tttggtgatt tgactgtagt gaaatctggg tctgcgacag
    21301 cttgttctgg aactagtacg aattgttcga atccagcttg tttcaacgca cgttcgccca
    21361 acatttttcc tgttccgtga agtggtgtgt acacaagttt caaatctttc cccattgttt
    21421 ccaccaattc atgatcaatc gttactgctt tgatttcttc tagataagcg gcatccactt
    21481 cttctccgat gatcgtgatc aatccgctat gttcagcttc ttcgtcagat aatacttcaa
    21541 cttttaacgg attgctgact tcacggacat atttcgtcaa tgcatctgcg tctgcaggag
    21601 gcatttgtcc cccatcttcg ccgtagactt tatatccgtt gtaagctgct ggattgtggg
    21661 atgcagttac catgattccc gcaaacgtct tcaaatgacg gactgcaaaa gaaagctctg
    21721 gtgttggacg tagactttcg aatacataag atgggatatt gtgatgtgct aatgttttgg
    21781 ctgcttccat ggcaaattct ggtgattgat ggcgtgagtc ataagcgata gcgacacctc
    21841 ggcgttttgt ttcttcgcct tgtgtatcca tgaaacgtgc aagtccttct gttgcttgtc
    21901 ttaccgtata gatgttcatt cgattgatcc caggccctaa gattccccgc atgcctgcag
    21961 taccgaattc caacggtgca taaaaagcat cttcaagttt ttcaggatct tcacccagat
    22021 catttaattg tttttttaag ttttcttcta aattttcttc atgtacccat tgttcataca
    22081 cttgttccca agacatgaaa ataacacctc tttctgaaat atggttctta ataagtatag
    22141 cattcactta ttagatataa aagtatgagg accaaaaaac gcttaaatca agaaaggatt
    22201 cttttatcaa gcacggttgc aagtattcgc ataaatcgga ttcagtagat ttcacgcgaa
    22261 aaaaaagagt aaaagaacaa ttgaaaccgt tcttccactc tttttaaact tatgcagttg
    22321 tttcttctaa agattccaag tatttataca cttgttgtcc agcgatccca ccatcaccaa
    22381 cagcagtagt gatttgtcgc aaatcttttt cacgaacatc tccgatggca aaaacgcctg
    22441 gtatattcgt cttcatttcg ttgtctgttg gaatccagcc agcttcgttc gtcaaccctg
    22501 cttctctaaa tggtgcagtt aatggatcca gtccaacata gatgaatact ccgtctgctg
    22561 ccaaatcaaa ctcattgccg gttttgacat tacgtgcttt cacacctgtc actaccattt
    22621 cattcccaac gatttcgtca acgactgtat cccacataaa ggaaattttt tcattcgcaa
    22681 atgcacggtc ttggatgatc ttttgtgcac gtaattcatc tctacggtgg acgatgacta
    22741 cttcagaagc aaattgtgtc aaatagatgg cttcttctac tgctgagtct cctcctccga
    22801 tcactactag acgtttgtta cggaaaaacg caccatcaca aactgcacag taagaaacgc
    22861 cacgtccagc aaattcttcc tcgccaggta cgcctaattt acggtggata cagcctgttg
    22921 cgataatgac tgtttttcct tcgtaagttt tatcttcaca aataacttct ttgtggctgc
    22981 cgtgatcctt gatatcttgc acgattccat aagcattttc tgtcccaaat cttgaaacac
    23041 cttcatacat cttataagct agatcaggac ccatgattga atcgaaacca ggatagtttt
    23101 cgatttctgc cgtattgttc atttggccgc caggggcgcc ttgttcgatc atcaatacgg
    23161 ataagtttga acgtgaagca tataaagcag cagtcatccc agcaggacct gcgccaataa
    23221 cgattacatc atacatatgt agattctcct ttacttaatc aagtaagcat actttacagc
    23281 caatgcgaaa aaaagtctat tgttctgctt gcgtttctcg aatagcttta ttatactcga
    23341 aaaatccgct gaaaaacaat agacttttat aaatcattga tcaatcattc gaaagatttt
    23401 ctccatttga atcaattact tttttatacc agttgaatga tttcttcttc gaacgggcta
    23461 atgtgccatt tccctcgttg tcacgatcga cataaatgaa tccgtacctt ttcttcatct
    23521 cacctgtacc agctgatact aaatcaatac atccccaagt cgtatagcct aatagatcga
    23581 ctccatctaa ttctactgca tctttcattg cttggatatg tgctgccaga tagtcgattc
    23641 gataatcatc ttccacataa ccattctcat cgggcgtatc gactgcacct aagccgtttt
    23701 ctacaataaa tagtggtttt tgataacgat cataaatatc attcatcgtt attctcaagc
    23761 ctaatggatc aatttgccaa ccccattcac ttgcttccag atatggattt ttgacagaag
    23821 caaagatatt tcctgcagtc tgttccaaga gttcgttgtc agtagtcgaa actcgagaag
    23881 aataataaga aaaggagata aaatcaacag tatgttcttt cagcaatgcg gcatccccag
    23941 gttccatttc gatctgaatg ccatttcgtt ccatttcttt caatgcataa gcgggatatt
    24001 ctcctcgtga ctggacatca atgaagaaat aattttctcg atctgatttt ctagaagccc
    24061 aaacatcttc aggattgcaa gtgtatggat aatatgctcc tgctgccagc atacagccta
    24121 ctttgttctc cggatctact tcatgagcaa tcttcgttgc gatggcactt gcaaccaact
    24181 catgatgtgc cgcttgatat ttgatttgtt cttcattttc tccttcttca aaatacagcc
    24241 ctgcgcccat gaatggtgca tgaaggatca tattgatctc attgaatgtc aaccaatatt
    24301 tgacaagtcc tttatagcga ttgaagatga ctcggcacag attttcataa aaaccaacga
    24361 gttctctgga acgccacgca ccatattctt tgatcaaatg catcggacaa tcaaaatgtg
    24421 taatcgtcac aagcggctcg atcccgtact tttgacattc tttaaagaca tcctcataaa
    24481 atttcaaccc agattcattt ggctcttttt catcgcccat tggaaaaata cgactccaag
    24541 caatggacat ccgataagtc ttgaacccca tttctgcaaa taaagcaata tcttccttat
    24601 aatgatgata catatcgatc gcaatttttg ctggataaaa atgattttca tcaaattcaa
    24661 acattttctt ttttccagta atgacaggaa aacggtcttc tccgattggc acaacgtcta
    24721 cgttcgctaa cccacgtccg ccttcatcat aaccgccctc gcattgattg gctgctgtag
    24781 ctccgcccca taaaaaatct tttctgaatc ccatcatact tcctccatta catgtgtatt
    24841 gcttgataat tctcttgcca gttcatcttg gacttcgatt ccttctgctt ttaaccggct
    24901 gattgtttcc ttgaattgcg gtaaatactg ctgatgtgca ataaacaact catccattat
    24961 ccgttttgct gtctttcctg atcgtataag cggattgatc gtaaacgctt gcaaagctgt
    25021 tccataatca ccagtcacgg ctgcttcgat cgtcagcaat tccatattct tcattacttg
    25081 caaccagcct ctttcagcag caggaaggga accaaacgct acatttcgtg cgccttgtgc
    25141 gccaacatag gcagtgactt ctactacaca atctgccggc agatcaggaa cagctccatc
    25201 attctttgta gataccacga tctgtgtttc tttatttcca tagatcgaag caatcgtttc
    25261 acaggcagca tctgaataat aagcaccacc tcgtttttgc aattgttcag gtttatgatc
    25321 caattctgga tctttgtata attcaaacag ctctgcttct gtttctttca cttgctgcgc
    25381 tcttgtacca atcgtttgat attcttctaa agcatgttcc agcatttctt cttcacggta
    25441 gtaatagcgg tggtagccac aaggaatcat gttcatttgt tttaattgtt ctttgaaaaa
    25501 cgggatatca aagatatttt tcggcaatcc attgtcttca tcgtataaac gatcaatcaa
    25561 ttctgccgtg cggtcatttc ctttattatc tgctactttg tgccaatgga agtgatttag
    25621 accggcaaac ttgtagatca gctcttcttt tttcttgcca agcattgttg gttctgtcat
    25681 catcgccatt actggcacat tgcataatcc gatcacttta tcccatttgc catatctgat
    25741 aactgcttct gtcaccatcc cgcttgggtt cgcgaaattg atcagccaag catttggaca
    25801 taaacgcttc atgtcttcaa cgattgccaa aaccactgga acggtacgaa aagctttgaa
    25861 tatcccgcca gcaccatttg tttcttgccc aatcatccca taataactag ggatccgctc
    25921 atctttgatt cgtgcatcta attgaccgac acgaaactga gtcgtaacaa aatccgcatc
    25981 tttcaatgct tcttcccgat ccagagttgc atagattttg acttcatacg gagaagcatc
    26041 ccacatacgc tgagacatct gactgacgat ctcaagcttt tcttttcctt cttctacatc
    26101 tacgagccag atttctcgta tcggtaattc ctcatatctt ttgataaatc cttccatcaa
    26161 ctcgggggtg taactgctgc ctccaccaat cgtagcgatt ttgattcctt ttgccatgtt
    26221 ctcactcctt tactttttac acttttatta tcttttttgt aaacaaaaca aacaatagaa
    26281 attcgtttct ttccagtaac atttacaaaa gttagtgttt tcttctggat ttttgtaaat
    26341 atcttgtcaa aaaatgtctt tattaagcta tataacataa atccgtttac aaatcatttt
    26401 caaaaaagtt ataatttacg taaaaaaaca agaagaaggt atctacatgt tattacgtga
    26461 aaaaatcaaa gaaatctcct tttctccttc agaaaatgaa gtgattcagt ttctcttaag
    26521 cgattctcca gcacttggcg aaatgaccat ccaagagatt gctgagcgat gttatgtcca
    26581 cccgtccaca ctcatccgtg tagcaaaaaa aatgggtttt gatggctggg tcagttttcg
    26641 taatgctttt ttagctgaaa cagcctatct tgaagggaat tttcaagacg tagatgcgaa
    26701 tcttcctttt tccaccacag acggtattat gacgatcgca aataaaatgg cttctttaga
    26761 acagatgacg attcaagata cactttctct cattcatcat gaccaactgc aaaaagccaa
    26821 acaactaatg cttcaagcaa aaaaaatcgt gattttcgcc agtaactcga atactttgat
    26881 ttctcaagat ttcatgctga aaatgaagcg catcaaaatg gatgtcacga ttgtcaccac
    26941 ttttggtgaa tcgttctatg aagcgtacaa ttgcacgagt gatatgtgcg ggattctgat
    27001 ttcttatacg ggtgaaaaca atgtgatcct tcgtaccgca gaaatattga aacaaaatca
    27061 tgtgccaatt ttagctttga ccagtatcgg tgattcaacg ctatcttcaa tgagtacagc
    27121 cgctttgcga cttacgacac gcgaacgact gtattcgaaa attggtaatt ttacgatcaa
    27181 tacatcgatt tgttatcttc tagacgtgtt atatagttgt gtttttgcag aaaattatca
    27241 acagaattta gtccatctaa tttctgtagg acaaaaattt gataaacgcc ctatcagctc
    27301 agccatcatg aaagagcctt tcaattttga aaataaagaa gtggactaaa aagaataatt
    27361 aagcctagtc cattctttca cctaaccgtt caggtcagtg cggtgataag tgcatcgtct
    27421 ggcccgaccg tctccccttg attttccaaa atatccaaat aatccgtgta attggtgacg
    27481 atcacaggtg tttctaaact gtatccttcc tcttcgattt tcttcttgtc gaagcggagc
    27541 aacagatcgc cttgtttcac tctttctcct tgctgcacca agcttttaaa atattttcct
    27601 tctaactgaa cagtatccaa tccgacatga atcagcaatt ccagacctgc ttcagaaacc
    27661 attccgattg catgtttcgt cgggaacagg gtcatgactg ttccgtcaaa aggggcacgt
    27721 acttctcctt tttcagggta gatcaagatt cctcgaccta atgttccttg tgcaaaagct
    27781 gcatcctttg ctgcattcag tggagcgatt tgtccttgta tcggtgtttt tacaatttct
    27841 ttacctgatg ttttctgtct atcatctgtt tcatctgctt cctccttcca aaagaaaaaa
    27901 gtcagcgcaa aggataccac catcgcaatg gcgatcccaa tcaatacatt aatcacatct
    27961 gttaaattac catctggtct aatatagttg ggaattgtaa agatgcctag tccaccttga
    28021 ttccaagaag ttaaattcat taacccacta tacgcgcccc ctaacgcacc accgacacag
    28081 gtaaaaataa atattttttt acgtggcaat gtaaacccat aaatagctgg ctctgtaatc
    28141 cccacaatac cagaaacaat ggctggtatt gcaattgctt tgtcttttgg atttctaagc
    28201 ttaaagtaga gtgcagtaag tgctgcagtt tgtccaaaag acactactgc aacaggtgtc
    28261 atgatatttt gccagccatt cacagaaaat tgcatcaatc ccattggtac gattgcccaa
    28321 tggagaccaa acatcattag aatttgccag aagaagccta cgattgctcc ataaataact
    28381 ggcgaaagtg ctaaaagtgt ttcaaatcca gccattaaaa tatcagttgc gaatgtgaat
    28441 gctggtccaa cgagtaaaaa acctaagggc attgtaataa gaacggtgaa gaatggaacg
    28501 aagaaattcg cgatcatctc aggtactaac tttttaattg gtttctgaat ttggctagct
    28561 aacaacacaa taaagataat gggaatgaca gaagaagcgt agttacgcgc aatccaggga
    28621 atgccgaata ctgtttgata aataggtgct tcaaaaatag tgccagaaaa tatggtcgtc
    28681 agtggtgctt ctgctgcacc actcaaagct tccagcgaca agctaggatt cattaatgct
    28741 gccccaagca acatccctac gaattcattt actttaaatt tacgggcagc tgttaaaccg
    28801 ataataacag gcataaattg gaatacagaa tcaccgatat tatcaaacat aacaaatgtg
    28861 ccagaaccgc ggtcaaatgc gcctaagaag acaagtaatg ctgtcagtcc acgaatcatc
    28921 ccaccagcag ctagaatagc aaggaatggc tggaaacagc ccgatattcc atcaataagc
    28981 atatcaaacg gtctcatttt ttctgttgaa acagcctcat caaaagtaag gtttcctgct
    29041 cttataacct cttcaaacac gactggcaca tgattcccga tgacaacttg gtactgtcct
    29101 cccgctcgca ttaccgtcac aacaccgtcc atatttttca agatctcatc atttgctttt
    29161 tcttcgtttt ttaatttgaa acgaagcctt gtgatacaat gtgtcaaacc attgatattt
    29221 tccttcccgc cgacattttc tacaattttg ctcgctaatt cttgatattt tcccatttct
    29281 attccctcca atttattagt ggaaggttta gccaactgaa tgtcactacc cttatttcat
    29341 gcgttcttgt taatttatcc tcctttttta tttatttaca acgattctca tgcactaaac
    29401 gcgcagtatg gatcgttaaa tacatttttt cgtccgtgga catctggtag ttgaatatct
    29461 cggctaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat tttttttgaa
    29521 taacttggta caattcctca gaaacttctc ccacttctga tttttctcca tctaggacgc
    29581 gtgtcagaaa aaaacgtagg tgtgtgacga atcgaaaaaa atggactgaa tcttcatcaa
    29641 atgtaatctt atagtaatag cggacgatat ttagaatatc ctgtattgtt gtagtaaaca
    29701 ggtaggtatt gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc aatgcgataa
    29761 atccggcttc atcttctgat aacttgatgt ggtatttatc cgagattttc ttcactgttt
    29821 tttccccgat ggcatactct ttaggaaata gttgtttgat atcatacagc aatacatttg
    29881 gtacaaatat tcccattttg tgacgttcga tagccgtata caaatgatcc gtcaatgcaa
    29941 tatacactga atcattcaac tggacttgta gagcctcttt tgtatattca atgatttcac
    30001 aagaaatttc taagtactca agaggaatcg cttgtgccaa ttcttggaac tgttctgaaa
    30061 gtccgtcatt tgtcaactga aattttttgt cgatgcatga gtcaaaaatc gggtcaccca
    30121 ctttcttttt gaatgccaaa ccttttccca tcacgatcat ttcctgattt ctttcatctc
    30181 tggttattac gacgttgtta ttcagtactt tctcaatcag catgttttac tcctttccat
    30241 gatatatagg gctgtatcca aaacaaaaag aaaaacccaa aaagccgtcc atggaaccat
    30301 gggagactca tcaggtttag cttgcatcag gcaatcacta tccaagttgt atcgttttct
    30361 ttatattatc gaaagcaagc gcttttgtca acgctttctt agtggctatt aggcacttct
    30421 gactagctgt cgaatctttt ttaaatctgg tgactacgac actaccgctc acagatgaag
    30481 aatctgggaa gctctgtttt attttcactc tagcgcttag gtcaaaatgc tactatgcta
    30541 ctgtcccaac ttctaatata cggtgttcca gtatcagctt atcggtgata agccaaattt
    30601 atgcaaaaat ctggaaagcc ttttttacaa tttttttctt atcacctaaa gctgaacgga
    30661 gccctgtcac aaccactttt tacacaccac tgctttgcag tttgtacata tctgcataca
    30721 aaccattttg cgctagaagt gtttcatgat tcccgcgttc tactatatga cctttgtcta
    30781 aaactaaaat caattctgca tcccgaatcg tggacagccg atgggcaatc gcaatcgttg
    30841 tccgtccttg tcgcattttt gtcaatcctt cttggatcaa tccttctgtt tcagtatcga
    30901 tatttgctgt cgcttcatcc aatactagta ttttaggatc tgtcacgatt gttcgcgcaa
    30961 aagtgatcag ctgtcgttgt ccgctagaat agctggcacc gtgttcgatc acttttgagt
    31021 gatatccttt tggtaattca tcgatgaact tgtttgcttg aacaaactca gctgcatggc
    31081 gaatctgttc gtctgtgata gttggattca tcatccggat atttcctgcg atatctccat
    31141 aaaacaagaa ggcatcttgc aagaccaatc ccattttctt acgtaactct tccattggat
    31201 attctcgaat gtcatgatca tcaatcaaga tctggccttc ataaaattcg taaaaccgca
    31261 tcaatacgtt gatgatcgag ctttttccgc ttccggtgtg ccccactaat gcaacggtct
    31321 ctcccggatt agcgacaaaa gagatattgt tcaatacatt gtgctctcca tcataagaaa
    31381 agcttacatt gcgaaactcg atcttacctg aagtaatcgt ttgatctgct ccaggatttt
    31441 gttgcggtgc atattcttct gtatccatga ttttcaaaat acgactaccg gcaaccattc
    31501 catctgtaaa ggtgctcaaa aagtccatca tttgagacat tggattaaaa aatccctgga
    31561 cataggtcgt aaatgcatag atcagtccaa cttctacggg agaatgcagc gcatcgatcc
    31621 caaacaaagt caacgccaac gcaatcgcca atgcatatag aaaattgatg atcggactta
    31681 acagaatcga attcgttttg atcatcgcat agcgcgtgtc taaatactcg ttgttcactt
    31741 gttcaaattc tttagctaaa cgagcttctt ggcgaaactg ctggataata cgcatgccgg
    31801 aaatcgattc attcaatttc gtgttcagct gacttaattt ttcccgcata ttccggtaaa
    31861 tacgagaact gaatttctgg taataccaga tgacgattcc taaaatcggc aagaacacca
    31921 aagtccacaa ggcaatttct ccattgattt gaaacatggc aatgaaggaa gaaacgactg
    31981 caaagatccc agtcaatacc atcaaaaaga cataccaaaa ttcaaatagc gtttctgtgt
    32041 catttgtcac tcgagaaacg gtcgatcctg ccggtgtctg atcaaaataa cgcataccta
    32101 gtgtatgcag cttttcaaat aatcttaccc gaacatactg ataggtcttc aatgaagcca
    32161 tggaatacaa ataccattgg aaaaaccaaa tgatactttt gactatgact ccaaacaagt
    32221 acataccagc aaaaaacaaa ataacttgtg tcgtagccga ctgtttagcc agatgatcat
    32281 ccataaaagt ttggatgatc cttggcaata ggacattgat cacagacaga gcaaacgcaa
    32341 ataagatagc aacgatgaac gttcttctaa aaggtttggc gaacttcatc agtcgtttga
    32401 tgatcttctt ctgttcttta agtggaatcg attttgtcca ttccgattga taattttcat
    32461 ccatcaggcc tcgcctcctt ctatcttcgc ttctaattgc tgtttctccc acattcgttc
    32521 ataccagccg cctaatgcaa gcagctcatg atgggttcct cgttcaatga ttttcccttg
    32581 atcgacaaca atgatttctt ttgcatgcat cacgctgctt aaacggtgag ctgcaatgat
    32641 ggttgttttc tcttttctta tttcttttaa attatgcaag atagcttctt ctgttttggc
    32701 atctactgca gaaagtgcat catctaagat cagcaattca ggatttttga tcaatgctcg
    32761 agcaatcgaa atcctttgtt tctgtccgcc agataaagag actccacgtt ctccaactaa
    32821 ggtatcataa ccttttggca attccttgat ttcattgttg ataaatgcta aagaagccgc
    32881 atgctctact tcttcttctg aaagttttgg attagaaaaa cggatattat ctcgaatggt
    32941 catcgaaaac aagaagtgat cttgaggcac atagccgatt gatcctaata aagcattcaa
    33001 tgaatattct ttgatcgaac gtccgccaaa ttggattttt ccttgataat catcgtattc
    33061 acgcatcagt aatttgatga tcgtcgtttt tcccgcacca gttttcccaa caatacctaa
    33121 tgtttctcct tcttggatca caaatttgat ttgttctaat gctggtgatt cttctttcgg
    33181 atagttaaaa gaggaaatag ctacattcag ctcgcctttt gccggggtct gcactgcacc
    33241 cagttgttcc acgatatgcg tcttttcatg caaaagttca ttgacgcgat cataagaagc
    33301 atttcctctt tccaatacgt tgaacaatcg accaatcgca aacatcggcc aaacaagcat
    33361 accgatatag ctaataaatg agaccatctg cccgatcgag atcgtgcctt ccaagacata
    33421 ctttccacct aaaataattg ttgcaacata agacagaccg ataatcaagg taatgaatgg
    33481 atcaaataag gagtctaaaa aattgactcg tttgttttta ataattgcat cttcaatctt
    33541 ttcttcaaaa tccgcgagat cttctttttc ttgtccaaat gttttaatga ctttgattcc
    33601 agtaatactt tcttgtgtct tatcattgat actagaaaaa gcttcttgcg aatcacgaaa
    33661 agcatcgtgg agttttgacc ccagcactcg tgaagtaacg gctagtaaag gcagaggaat
    33721 cagcgcaatc agtgttaagc gccaatcaat aaacaaaatc atcgcaatga tcgttgttcc
    33781 gcccgtgatc actgaatctg caaaggtcaa gataccagct cctgctacat tttggattgc
    33841 attcaaatca ttcgttgcat gagccatcaa atctccagtc cgatattttt ggtaaaagag
    33901 attgtccatt tctgtaaaat gtttgaatag ctgctgccga agttctttct ccaaccgagc
    33961 tgcacttccc caaatattcg tccgccagat ataacgaaaa agatattgta agagagctgc
    34021 aaggatcaat aataagatcc accaggaaat gatctttatt gataaatttt tatcggcaat
    34081 ctcatcaatg atgatcccaa tcacctttgg aggaactaac tgtaccaagg ccactgcgaa
    34141 tagggagaaa atcccaatta aataatgttt cttttcctgt cgaaaaaacc aacctaattt
    34201 tctaaaaatc gacattttac tcctcctttg tctttatcaa gagcatagga ttgtctgtgc
    34261 ttgtcttttt tgctgaatag tctgaatagt ctaacaaatt acgaaataaa aaacggactg
    34321 aaaaaatcag cccgcaaaaa gtctttgggt cagaacctat tttttccctt ggttcttcat
    34381 ttgctgcatc atctggcgaa ttttcttctc agatggtttt tgccccattg acatcatcat
    34441 tgatcgcaac atgtcctcat taacgggagg atttttttct aaatagtctt tcatatattt
    34501 acgcgctagg aaaaagccac ctactgcgcc gattagcaaa gcaataatag caataagtac
    34561 gacaatacct gttgatacca tataaatctc tcctttctat ttccgaattg ctctcaatga
    34621 tttattttac taaagtttat tgaaaaaaga aaggcgtatt ctttagtttc atgcaaaaaa
    34681 tagtggtttt tttagagaag aacaaaattt ctttcattaa ccagcaagac acgccacgaa
    34741 aaaaaattag gcataccgaa tatttttctt taattttttc tttctgaggt gtagaatgta
    34801 atcgtagata aataggaaag gtggtttttt cttgaataat caatccaaca aacaatttca
    34861 taagcatcga cttattgaac atacaaatgg tttatcttta gctgaagtaa atggaacagt
    34921 ggatgtaccc aagggaaaaa gtttttttaa aacacttctt gcttactctg gaccaggtgc
    34981 attagtagca gtgggttata tggatccagg taactggtcc acttcgatca ctggtggaca
    35041 aaatttccaa tatttattga tgtcagttat tttgatgtct agtctgattg ctatgctttt
    35101 acaatatatg gcagcaaaat taggaatcgt gacccaaatg gatttggccc aagcaatccg
    35161 agcacggaca agtaagacat tgggtgtcat tttatggatc ttgactgaat tagccattat
    35221 ggcaacagac atcgcagaag tcattggtgc agcaatcgcc ctctacctgc tttttgatat
    35281 tccattagtg attgccgtat ttatcactgt gttagatgtt ttcgtcctct tattgctgac
    35341 aaaagtcggg tttcgtaaaa tcgaagcttt ggttgtctgc ttgattttcg tcattttatt
    35401 cgttttcgtc tatcaagttg gcttgtctaa ccctgattgg cacagtttgt ttgccggctt
    35461 tatccctaac agtcatacat tctcagactc accggtcatc ggcggtgaaa ctccgctgac
    35521 tggagcttta gggattattg gtgcaacagt catgccgcat aacttatatc ttcattcagc
    35581 agtttctcaa actcggaaga tcgatcacca agatgaagaa gatgtagcac aagcggtacg
    35641 cttttctact tgggattcta atatccagtt gtcaatggct tttctagtca atgcactgct
    35701 attgatcatg ggtgtcgctg tattcaaaac cggttctgtc caagatcctt ccttttttgg
    35761 tctgtatgaa gctttatctg atccatcagc tatgagcaat ggtatcttga ttgctgtcgc
    35821 aaaatctggt atcttgtcca ctttatttgc tgtagcactt cttgcttctg gccagaactc
    35881 tactattact ggtacgttga ccggtcaagt catcatggaa ggcttcatcc atatgcgtat
    35941 cccgatttgg ctaagaagac tagtcacacg attgctttct gtgattcctg tattgatttg
    36001 tgttttgttt acaagtacaa aaggaacgat cgaagaacat accgctttga ataacttgat
    36061 gaatgaatcc caagtttttc tggcttttgc attgccattc tcgatgattc ctttactgat
    36121 gatgaccaat agccaagcgg agatggggag ccgattcaaa aattcatttg ttgtaaaact
    36181 acttggctgg ttctctgtga ttagtctgac ttacttgaac ttacgaggat taccaggtca
    36241 aatcgaaagt ttcttcggcg agcaagccac ctcagctcaa ctagcattgg ctgatcaaat
    36301 cgcttatgta atcattgttc ttgtccttct cctgttggca tggacgattg gtgatttata
    36361 taaaggaaac aaaaaatatg aacagcgttt gcaagaaacg atagaagaaa tcgaatagaa
    36421 actgattctt ttacacaaag gaggttgtga cagaagtggg cagcttcaag aaataagaag
    36481 gaatttaaga aaattgcttt tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt
    36541 tacttctgga acaccgtgta tcagaggttg tgaaaaagct gtcctgcatc aagtaataag
    36601 aacagccaaa caaaaatagt tcctcatatt ttacgtttgg ttgagcttat taccgcagat
    36661 gcaagctttt gaacaccgtt tattcggaaa aaggaactgc gacaagactt ttgtcacagt
    36721 tcccttttta tttagaagat aaagcttttt tcagattcaa tttactgaaa actagcatgc
    36781 ttgttactac gatgaattgt gttaatggca cgcctgcgcc agtgtagaca cttaaaaaat
    36841 aatccttttt ttctggcgca taagaacaaa accacgagac accgaaaaga aaaaccaatt
    36901 gtgtcagtat taatccggtg actacaatca aagaggaact ataataggca cgatcagctt
    36961 ttgcaaatac ggttcctagt atcataagta atatccctgc tattgaagca atcagcacgg
    37021 gaatggccag aacttggtaa atttttatca tgacattcgc gacttttgct gtttttgtca
    37081 actggaaatc aggtgttctc ggatcactgc tatcgatcca attatccatt agtggaagat
    37141 tcaataattc ttgggcctgt tcgacttgtt cttgcgtccc gaaactataa ttccctcctt
    37201 gatggaaatc tttgtatcgc catgcatagg catatcctga tttcagaaaa gcaaatacga
    37261 ttggcagatc attcatcgtc tttccatccc ctgatcccat cagatagaat tcttttttct
    37321 ttttgatagt tcccttttga taggcttctt ctaattcttg attgactttt ttccaaaaat
    37381 cattggtttc ttttgcattg ttttggtaat aaccagattc gcttaccaca tctcgcaatg
    37441 cccaaaaaat gatatctcct ggtaattctt ttacttttcc gccttgggtc cacgcatgag
    37501 tcgtatacaa ttcgtcaata cgagtagaaa cagtatgaaa tgtaggagat gcgtctaatg
    37561 ctttatttaa tgcagcatgc gagacccata tctcactgtt ttctttattt aagttcgttt
    37621 cgtcatctat tcggatcagc tgtttcatca aacgtccaaa ttcaccactc gaacggtcat
    37681 tgatggtggc aataccatat gttgtctcgt ttttatattc gatccactga tagacgatgg
    37741 agaaaccgac tagagggagt aaaaacaatg aaagtttcag ccattcttgt ttcgaaagga
    37801 gaaatggtcg ttcttttaac ttctttgctt ctttgaaaga aaagtcttgt cctagcaaga
    37861 tctgaacgaa actgatgcct aacgcgacta cagtaaaagg aagaagccag atggaatctt
    37921 cgcgaatgaa ccaaaagaaa ggaaaagaaa cagctaaccc taaaaaccac ggcagcaatt
    37981 ttctgcggga actatattta cggtaataaa tagctagtaa acaagcgata atgatctcaa
    38041 cagccgggat cacgagagca ttacgatacg ttcgagtgga atactcatgt gtaaaactaa
    38101 tcggagaaaa caaaaaataa aaatagatac ttaaaagaag atacttgttc ttcacgatcg
    38161 gtttaaacgc tagtgcaacc aatatgctgg caagaatact gaacatgccg aacaaaactg
    38221 cataagaaag atgcagttta tgtgcaagta ctaaaaacaa gctgtaggag atcccttttg
    38281 aaagtgtcgt agtcgtgtac tcccctagcc atttaccatc caaaatattt tgagcatagt
    38341 gaatcagcag ctgatcatca taggttgccg acatatctgt tccatagatt gctttactat
    38401 ataaatacaa ccgaataatt gtaaataata cggctatgcc aagaataaag tatgtatgtt
    38461 tcttttttag ttggttgatc aagggtttta aagtgttcag catcttttcg tcctttcaat
    38521 cacgttttgc tacttgcttg tgaatctttt cgttttttac ttttctatca gtataccctg
    38581 tttgtctgaa tttaaaaaga gggggcgaaa ggagcatggc gaaaacgact gcaaaaagca
    38641 ctcctttgtt tcttaaacaa aaaaggacaa ggcagactgc ccaatccttt tttgttgctg
    38701 tcagatagaa aacttaaagt gtgacttctt ctgctaataa ctcaggaaac acttcgggat
    38761 cgaatgcttg cgctttgaac atggcaattt cttttccata tggtgcgtaa gctttctttt
    38821 tatctttacc gacatatgga gtttctaaga ttttcgaaat gttgtcaaac attggatcat
    38881 ggacgatttt gttcaatgtg tcaaatccaa ttgttccaaa accgatattg gcatgccgat
    38941 ctttatgaga accttgtgga tttttagaat cgttgatatg gagtacttta agccgatcta
    39001 atccgatgac cttatcaaac tcttccatca cacctgagaa atcttctttt atattatatc
    39061 cggcatcatt cgtatgacat gtatcaaaag taacggataa tttttcattc aattttactc
    39121 catcgatgat ttgcgctagt tcctcaaatg ttcgaccgat ttccgtacct ttccctgcca
    39181 tcgtctccaa agcgatctgt gccgtctgtt cttttgtcaa aacttcattc aaaccctcaa
    39241 taattctagc aatcccacct tctgcccctg ctcctacatg agcgcctggg tgaagggtga
    39301 tttgagtagc acctaaagct tccgcacgaa caatctcttc tctcagaaat tgtacagcaa
    39361 atggaaacat ttctggtttg atcgtatttc ccaaattaat gatatagggc gcatgaacga
    39421 cgatatttgt tagtccgttt tctgccataa atgcttttcc ggcatcaata ttcatctctt
    39481 cgatagcttt tcttctagta ttttgaggag cgcctgtata gatcataaag gtcgaagatc
    39541 cgtaactggc tgcttcttct gccgcgccta acagcatttt ctttccgctc atgctgacgt
    39601 gtgagcctaa taacatggat ggattcctcc ttgaaaggca attgtttcga acaattccta
    39661 tatattggta agatgatgta caaagaaaat ggtttgtttt tctttgccct gttctccata
    39721 gtaactgaaa ctttctctaa agaaaagctt aagaactctt tcataaaaaa gatcatttcc
    39781 tcacgtttta tctcattcga aaaagaaatt cctgtcaaaa acagccttgt ctgttttttt
    39841 gtcttattat ttgctataat tagcgaaata aaaccattag ttgaggtgac ccttttgcca
    39901 cgcaaaaata ctcgaaaaaa acagaataga agaaaaaaag aaaaatggtt tgttcctaag
    39961 gttattttta gagtgtttca atcattgact gtttttatta ctgttcttct tttgttgttc
    40021 gcagctctag gtatcggaat aggtgctggc tactttgcct atcttgtcga agacacacaa
    40081 ttgccgacta aaaaagaact gcagacagaa ctaggaaaca ttacggaaac gtcaaagatt
    40141 gtttatgcag ataatacaga gatatcaaaa atccagacag atctgatgcg gacgactatt
    40201 tcatctgata agatttcgcc tttattgaaa accgcgatta tcagtacaga agatgaatac
    40261 ttcgataaac accaaggtta tgtccctaaa gctgttttac gagcattagt ttcagaagct
    40321 actggcatcg gctcgtctgg aggatccact ttgacacagc aattagtcaa acagcagatc
    40381 ctaaccgatg aaacaacatt caaacgtaaa gcaaacgaga tcgttctttc tgcacaagta
    40441 gaaaaatact tttcaaaaga tgagatcatt gctacgtatt tgaatgtctc gccatttggt
    40501 cggaataata aggggcaaaa tattgctgga gtccaggaag ctgcaagagg aatctttggt
    40561 gtagatgcaa aagatgtgac tctgccacaa gctgcttata tcgctggttt gcctcaaagc
    40621 cctatcacgt atagtcccta cacgaataca ggcgcgctaa aagaagatct ttctgccggt
    40681 cttgccagaa aagattttgt tttattcagt atgtaccgtg aaggacaaat tacgaaagaa
    40741 caatacgaag aagcgaaagc ctatgatctt acaaaagact ttctcccaca acaaatcgcg
    40801 gaacagactg acagagaatt cttgtattat accgtgatga acgaagcaac aaggattatt
    40861 gcacaacaat tggcagaaaa agataatgct gatatgtcag attcatcggt cagtgaagct
    40921 tattatcaaa aagcacagca aacgatccaa aacaaaggat atacgatcca ttcgacgatc
    40981 gataaagata tctatgctgc attgcaaacc ggcgtagaaa attacggcta tatgttagat
    41041 gatggtgctg gtagccaagt ggagactgga aatgtattga tggacaatag gactggacgt
    41101 atctatggat tcgtgggtgg ccggaattac agccagaatc aaaataacca cgcctttgat
    41161 acagaacgtc aagctggttc atccatcaaa ccggttcttg tttacggtcc tgcaatcgat
    41221 atgggacttg tcggatcaga atcccgtgta tcagattatg caacgacttg gcaagaagga
    41281 gaaaatgctg gcgagaaaat cgtcaatgcg accaatgaag ggtcgaacac cttccaaacc
    41341 gtccgtgagt cactggaatg gtccaacaat atcccagctt atcacttata ccaagacgta
    41401 ttgaattcgg gtggatcgaa acaatatgct tacgagcact acttggcgaa gatgaactac
    41461 ccagcaaatg ataattgggg cgttgaatct gctcctttag ggacagtaga tgtaacgacg
    41521 cttcaacaga caaatggatt ccaggcctta gctaatggtg gcgtctacga agaaggctat
    41581 atcattgatt ccatcacaga taatgcaggt aatgtgattt ataaacatga aagtaattct
    41641 gtccgtattt attccgaagc aacggcctcg atcatgaatg acatgatgcg cagtgtgatc
    41701 aatgaaaaaa tcactacacc attcaaagat gtcatttcaa gcttaaacgg aaatctagga
    41761 aaagcagatt gggtcggaaa aactggttct accaacgaat acagagattc atggctgatc
    41821 gtctctactc cgtctatcac tatcagtagc tgggcaggac acgatgacaa tactggaatg
    41881 gacagccaag caaggatacg ttcggctaat tatcttgcca atctaatcaa tcaagcttac
    41941 caagcaaacc ctgagatttt cggcactgat gagaaatttg acttatcatc tgatgtgatg
    42001 aagaaaaaag tatctgggtt cacaggacaa acagctggaa aagttactgt caataaaaaa
    42061 tctatccaaa cgccaggaaa aacagtggaa tcactttggg caaaaaatgg tccgaagaaa
    42121 agcgccttta aattcggcgt tggcggtact gatgaaaact atacagatta ctggaacacc
    42181 gcttcagctt atgctagagc gaatccagag accaaagaat cagacgataa agatgattga
    42241 tctaatagat taatcagaat taaagggagc tgcgacaaaa atcttgtcac agctcccttt
    42301 ttccgaataa atggtgttca aaagcttgca tctacgatat taagacaaat ttatgtaaaa
    42361 atgtgaaaag ccattttcac aaaatttttc tatataactt tactaacgaa actgttttaa
    42421 tttcttttct tgtctttgga ataattcggt tagtttttct gcaaattgcc cttttactga
    42481 ttctttcaag tgtacatgtc ggttagaaaa ttcttgagca gcttcttctg cagtcatttg
    42541 gtggttttga tctgtcaatc gtttttctgc ccaaatttgc tgagatatcc ccataccaac
    42601 tgaacgtaca tttgatcgat gtagattttt ctctaattct tttagtaatt gcattaaaaa
    42661 ttcattcata agatgatttc tccatagtta tttgtctgat tttttgacga taggaacgtt
    42721 ctagttcgtc ttgctgctga tagagttttc gtcgttctac ttgtagggct tgttgataat
    42781 gctcctgata attcgaataa cttcgtcttg cttgaaacag aggctcatta tctaaagcta
    42841 agttctttaa tacatgatgc agtcgctgct cctcttgctc catcaatcgt aatccttgat
    42901 tcttaaagtg gatcaattca tcttcttgag cttctaattg tcttcgttca cttcgataat
    42961 ctctttccaa ttcttctagt ttcatctact tctcctttaa ttgaattgct gtgctaattc
    43021 tcgatctcgt gtcacgattt cattgatttt gtcttggatc tccttcacca atgtatcaaa
    43081 ggcctccccc atacgtcttg ccttattgat tcgcagcaga tagacttctt tcgcagaatc
    43141 gactaagttc tcttttgtgg cgacaacact agctaatgct tctatgattt cctgttcagt
    43201 caacgaaggg gcgacaacac gtgcttgccg caagccttct tgccatgcca ttttttctct
    43261 agctcaaaaa ttcctctttg attgatgcgg ataacttctt ccatagcgct tttcattttt
    43321 gctgataact tttccactac taacctagct tgtgtgtcat ctaagtaaat ttgttcatta
    43381 actgtcagcc ggccgccact taatcgtagt tttttacgta tgttgttcag ttcttgcaac
    43441 tgataggaaa acaactgggt cgcttggttt tctttctgtt gttttcgagc tttaacatta
    43501 ttttcatttt caggaataat cagctttcca ttttcatcaa acttccaaga ggttaaactg
    43561 tgggaagcaa gcagattcaa gaatgctgtt tccatactga tccgaacttc agcacctgtg
    43621 ccattaccac caaaattacc gataaaatcg gcgtgatttc ggtaattggt gagcataccg
    43681 ggattttctt ccacccattt ttttgcttct ggtgataaga gatcatacgg gtccggcccg
    43741 tttaagccga catttttcca ttgtttctct gctgctacat ataatgctaa gaatccacct
    43801 aatgaatggc cggtagtaga aatagcagct tctccatatt ccttttttac ttcattagca
    43861 aatgcaatcg ctgaattgat ttgccccgct gttttcaatc cactaacgga taaaatcttg
    43921 tttgttgttg ctttttcaga aaaacttgct cctggtctat tcccatagag attcatgata
    43981 ctccttgcat ccgtttccaa atctttccaa tcacttaagt tcgttcctgc aaaagcaatc
    44041 actacttgag aaagatccac tttaccgttc ttgattggcg ctacacccat cgcctgcatg
    44101 ccgtccaatg agtctttgat ttttaggatt ttaaaattat aactatcaat ttttttaata
    44161 gaattttctt tataagtatg atcatagtct ctgtgtttag gatctagcca gtaaacagcc
    44221 tcactaataa cactgtattg tttgtctgcc atcattaaat atattcctcc ttattattag
    44281 aaaaaattac tctgatttta tttactgtaa tcccctcttt ttgtatactt ttattttcta
    44341 cgccataatc tcctaattca tttcgttctt tccaaaatcc aaacgtaaaa taaacagact
    44401 catcgtgatt atttatcatt gtcacaaaca tacgatatgc tcccgtcatt tcatttcgag
    44461 cagatttttc aatcttcact tccttgatat ccgcaaaagt atttttcaaa actttgactg
    44521 actgtttttc tactgcgagt aaatctttct gtctttgtgc ttctcttttg tccatataga
    44581 ctttccctcc gattcctaat atgacgatta ataaaatggc taagctgagt tttcttattt
    44641 tccgagacaa gaacgtgcct cctttctttt tatgtaagta ttgatacctt cattatttaa
    44701 gtacgcgaaa gtttgtagga atttttttat ttttgaaatt tttctccaat ttgctatgga
    44761 cgattggcgc tgcagccatc gcctgcatgc catccaatga gtctttgatt tttaggattt
    44821 ggaattttgt ttctccaaag tttttaatgg cgccttcttt gtaactttta ctatattgag
    44881 gatgttttgg atctagccaa taaacatgtt cacttatata tctatactct tcgtctttta
    44941 ctgtcacact tcctccgcct ctccattaga ataaataact cttattttag gagtagtaac
    45001 cccttcgttt tgtatttctt cattttctac accataactg ctaatttcat tcatttgttt
    45061 tgaaaaaaaa taatcaaaat aaacagattt cccttcatta tttgtcattg tcacaagtgc
    45121 accataacat ccagtaacag ggtttttttt aaattcttct accttcactt ccctgatatc
    45181 tgcaaaggta tttttcagaa ctttgacgga ttgtttttct actgcgagta aatctttctg
    45241 tctttgtgct tctcttttgt ccatgtaaaa ttttcctccg attcctatag caagaatggt
    45301 caaaatgatg actatggtct ttcgtctttt cttaggcaaa tagaagtctc ctttcgggtt
    45361 atgtatgatg gattgtttct cctttaagat aagtacgcaa tcgagcctgg atttttttgg
    45421 attttttaga gttacttttc tacttttcta ctttcttttt tatcacgttt tcattttaaa
    45481 gtaattttga tagtagtgaa caaaatgtag catctacgca cagattgtgt cgtatacgat
    45541 ggacgtaagg tttgtttccg ccattttctc tttttttaaa gtcacaaaaa aaaggggtta
    45601 tgacaaaatt ttgtcataac cccttttctc gaataaacgt tgttccagaa gttggccact
    45661 tttggaacaa cctctaaatg attgatcttt tattatttag ttgatccttt ttcttcgatt
    45721 ccataaggca agataatcgt tttttcttcg acttcttctt tattcatcat tttcgtcaat
    45781 aggcgcatgg ataccgctcc taaatcgtat aatggttgtg tgatacttgt caaacgagga
    45841 cgagctactt ctgtcagtaa agaattgttg cttgtgatga tttcgaattc ttctggtact
    45901 tttacgccac ggtctaataa accatccaag ataccaatgg ctaattcatc gtctgaaaca
    45961 tagacagctg ttgcaccgct attatggatc cggtcaacta gagacagacc atctttaaag
    46021 ttgtattgtg cctcaaagat taatccttcg ctatatggta gattgttttc gcttaatgct
    46081 tctttatagc cttttagacg gttttgacca ttgataggat caatcagcgc accgctgaca
    46141 aaagcaattt ttttattgcc gctttttgct aatttattta ctgcatcttt tgttgctgat
    46201 acataatcaa tgtttacgct acctacttgt tcatctggat caatggaacc agctagtaca
    46261 acaggtgttt ttgaacgtga aaattctcca cggatttcgt ctgtgatatg gtgacccata
    46321 aagatcacgc cgtccacttg cttagccaat aaattattca atacattgac ttctttttga
    46381 tcgttgccat ctgagtttgc taagatgata ttgtatttgt acattgttgc tacatcgtcg
    46441 ataccacgtg ctaaagaggc aaagaacata ttgctgacat caggaatgat taccccaaca
    46501 gttgttgttt tcttacttgc caagccacgt gcgactgcat ttggacgata atccaagcga
    46561 tcgataactt ccaagacttt ttttcttgtt gctggtttga cgtttggatt gccatttacg
    46621 acacgtgaaa cagtagccat cgaaacattt gcctctctcg ctacatcata gatagtgatt
    46681 gtttgctttt ccatttgtgt tctcctattc atattttata tgttttctga aaatacgtct
    46741 tacatttcta aaataggata tcaaataatc tcaacaattg caaccgtttt atctctgttt
    46801 tcatgaaaat ttttactttt ttcagtttta agaacgttgg tcttttaatc aatcagtggt
    46861 acaattattg aatacaaaat taatttagaa agtaggttgc caaatgaata acgtaaaaat
    46921 caatgaacta cgtcaatgga tggcacaaca ccaagtagaa ttggcttata tcagcgaccc
    46981 tggccacatc gcttactttt ccggatatga aagtgatcct cacgagcgtg tactagctct
    47041 ttttatccct cttgaacaag atcctttcct tttcactcct gctctggaag ttgaagatgc
    47101 tcaaaacagc agctgggcgt acgatgtacg tggctactta gacagcgaaa acccttggga
    47161 gatcatcgga aaagagcttc gcgcacgcta tgggcaacct ggtaatgtag ggattgaaaa
    47221 gaatgctctg actgtcgatc gcttcgatgc tatctctgat atcttgacag gtgcagattc
    47281 ttacactgat ctaacttcta ttatccagcg tctgcaactg atcaagacgg tagaagaaaa
    47341 agacaaactg atcgaagctg gtaaatgggc cgatgttgcc tttgaaattg gcttcaaaac
    47401 agtaaaagaa ggcgtgactg aacaagcaat tatcgcagaa atcgaatacc aattaaaaaa
    47461 acaaggtgtt tcacaaatgt ctttcgacac cttagtcctt gcaggagcaa atgctgcaag
    47521 ccctcatggt acaccaggaa gcacgaaggt ttcaccaaat gaactagtgt tattcgatct
    47581 tggcgttgtt tggaatggct attgcagtga tgctactcgt acagttgctt atcaaaaacc
    47641 aacagaattc caagaaaaaa tctacaacat cactcttgaa gcacagcttg ccgcacaaga
    47701 ggcagttcgc ccaggtgtga cagcaggtga acttgatcaa atcgcacgaa acgtaatcaa
    47761 cagctatggc tacggcgaat atttcaatca ccgtttaggt cacggaattg ggacaaccgt
    47821 ccacgaattc ccttctcttg tagaaggaaa cgacttagtg atcgaagaag ggatgtgctt
    47881 ctctcttgaa ccaggaatct atattcctga aaaagtaggg gtacgtatcg aagattgtgt
    47941 gtatgtgaca agtgatggtt gtgtgccatt cacgactaca ccaaaagaat tacttgtttt
    48001 agagtaatga aaataaaaaa ggtgggagct ggtccgctct caccttttta ttctagaaat
    48061 gagtattctt agtgattggt agtcgaatct gtcattgtat ctttcatttc ttttttagct
    48121 tcttttgcat cttcttttgc cattttcaac atttcttttg tgtcatctgc aactggtttt
    48181 gcttctgaca ttccttcatt gaccgtctct cctgctttat ctgcagcatc ttttacatcg
    48241 atcatgatat cttctgaagt atctttagca atactaccta attcgtctgc tggttttttc
    48301 agatcttctg ctgttttttt gaaacgatta gataaatcag ctgattgttc tttgaggttt
    48361 aacaagatat catctgattc acttcctgct tcctctgctg attcacttac cttttgctta
    48421 acattacctg ctagatcccc tgcttgttga gaaagatctt gcgctttttc ttttgcaatg
    48481 cctgttaact cagctgattt ttcttttgcc atatctgtgt aatctgaagc catgtccatc
    48541 aaatcatctg cttgagcagc caattcatcc cgaagttctt taccagattt gggtgctaac
    48601 aataatgcgg cgattgctgc ggctgtacct ccaactactg cacctaataa aaatccacct
    48661 tttttagcca ttcttatttt cctcctgtcg tttcagtatg ttttttgttt ttaaaaaatt
    48721 tcatcgctgt tccaccgact tttccggcaa cagtcgcttt agctgtcgtt tttccgattc
    48781 cgccgacttt cgtcacgatg tttttactag aatgattaag ttccgataca ctttcactta
    48841 aatcagcaac tgctgtgaac aatggatcga tcgttgcgac ttttccattg atatccaaga
    48901 gcagctcatt acttttgacc agcaagcctt ctacttgtct agataaaaga tctacgtctt
    48961 ttgtgacgat ctcaattgtc gtatttgctt ctgcgacagt ctggtctact ttttctaaag
    49021 ttttggaaac ttgcaacaat acgcgaatga tgaaaacgac aagtatcgca aaggcgattg
    49081 ctgcgatcaa tcctgcaatt tctcctcctg acatgatcaa actcccttct tgtctctctt
    49141 ttctataagg atagcatttt cgtgtttttt aaacaaataa aaacgagctt caactacttt
    49201 ttattcaatt agcagctgaa gctcgttaga aaaaaatgat tattttttct tttcttcttt
    49261 gatttctgtt attccttttg gattatgatc gatcttgctt ccttctgctt ctaatgtttt
    49321 acctaaagac atgatgtatt cacgcaaagg tgcccctact tctggatgtg tcagaccata
    49381 ttcaatactt gttttcataa aaccaaattt gtcaccaacg tcataacgtt tacctgtgaa
    49441 tttacgggca aatacgcgtt gtgttttatt caacgtatcg attgcgtcag ttagttggat
    49501 ctcatttcca gcacctggtt gttgttctga taatacatca aagatttctg gagtcaataa
    49561 gtaacgccca ataatagcta aatcacttgg tgctttgctt ggatctggtt tttcaacaaa
    49621 gtttttgaca ttgtataacc catcttccag tacttcccct gggttgataa ttccgtattt
    49681 tgaagtttca tcgtgaggaa cttgcataac ggcaatcgta gaagcgtgtg tacgttcata
    49741 gtcatcgatc aattgctgtg tcaatggtac ttcgtcttcc atgatatcat cgcctagcat
    49801 aacgacaaac ggttcatttc caataaaggc tcttgcttga agaacggcgt gtcctaaccc
    49861 ctttggatga gattgacgga tgaaatgcaa gttgacatct gtcgtttctt ctactaattt
    49921 caataattcg atttttcctt tttcacttag gtttgtttct aattcgatat ttgaatcaaa
    49981 gtgatcttcg attggtcgtt tagcttttcc tgtaacgatc aagatgtctt cgattcctga
    50041 tttcaacgct tcttcaacga tgaattggat cgttggtttg tccacgattg gcaacatttc
    50101 ctttgccatc gcctttgttg ctggtaaaaa tcttgttcct aatcctgctg ctggaataac
    50161 tgcttttttt actttcatat acattcgctc cttcaaaaaa ttgttaatga gaattcattt
    50221 tcttttttcc catcacgtaa catgatttct tttgcggctg ttttgatttc tttttgttca
    50281 tacagcactt catagattgt ttgagtgatg ggcatttcga tttccatcat ttgggctagt
    50341 tccatcgctg cttgagtagt attcacacct tcaacgacca ttcccatatt ggctagcacc
    50401 tcttctagag gctgtccttg tcctaacaag ttgcctgcgc gccagtttct ggaatgaaca
    50461 cttgtgcaag taacgactag atcgcctact ccgcttaagc caataaaggt cagcgggttg
    50521 gcacccattg ctacacctaa acggctgatc tcagctaatc cgcgcgtcat gattgccgct
    50581 tttgcattat cgccaaaccc taagccgtga atagcaccag ctccaatggc aataatattt
    50641 ttcaaagctg cccccatctc tacaccaacc acatcagggt tagtatagat tcgaaaataa
    50701 tggttcatga acagctcttg tacatatttt gctgcttttg catctgtgct tgccgctgtg
    50761 atcgtcgtaa tgtcatgctt tgctacttct tccgcatggc ttggcccaga aagaacagca
    50821 acagcttttc ttttttctgt cgggatttcc tctaacaaga tttcagagat tcttttatgc
    50881 gttccctgtt ctaatccctt acttgcgtgg atgatcactg gcttcgtatt caatttggca
    50941 actagttctt ttgcaacaga acgtatcgct ttcgtcggga taacgaatag taccgcatct
    51001 gcatctttta ctgcatcttc catatcggtg aatgctcgga tggaatcggg tagatgatga
    51061 tctggtaaat aacgttgatt ggtgtgttgg gtgttgattt cttctacttg tgaagcacga
    51121 tgtccccaaa tccgaacgtc atgtccgttt tcagcgagga cttgtgctaa agcagtcccc
    51181 caagagcctg gtcctaaaac cgctactcta taattcattt taccactcct gaaatttttt
    51241 tcatttttaa cgctctaaaa atcaaataat ttgttgattt tttcctaatg agcggtcata
    51301 atccggtaaa tttccttttt ttctacgcca aataaacaga atcaatgtgc cgacaagtaa
    51361 aacagcagac aataattgag aaatgcggat cacacctaaa aagtaaaggc tgtctgtgcg
    51421 taatccttcg ataaagaaac gtccgaaact ataccacatt agataacata aaaggacttc
    51481 tccttttttc aaaagatgcg gtttcttccg caaaaccaaa agcagaataa atccaatcaa
    51541 actccaaagt gattcataaa gaaaggttgg ttgtcgatag atcccttcga tatacatgtt
    51601 atcaatgatg aattgcggta aatgcagact ttctaagaaa cttcgtgtcg tgtctggacc
    51661 atacgcttcg tggttcatga aatttcccca tcgtccgatt gcctgcgcca gtaatacact
    51721 tggtgcagcg atatctaaaa aggtccacaa agaaataaaa tgatatttgg taaaaaagta
    51781 cagtgtgatt gcacctgcaa tcaatccccc gtaaatcgct aatcctcctg aacggatatt
    51841 gaatatttgg attggatcag ctatatattg gggaagatcg aataaaacat aatacaaacg
    51901 ggcaccaata atggaaatcg gcaatcccca caacataaaa tctgtcacgt catctgcttt
    51961 taatcctacc cgaacagctt ctctgctgct gagccacatg gcaataatga ttcctgatac
    52021 gataatcaat gcataccagt agataggaat tcctacaagc tcaaaagcaa ctcgattgat
    52081 ttgtgctaac atattgtcac tccttgctag tcattgcctg agttttcttc aatcaagcga
    52141 cttaatcttt cttcaaatgt tttcgttgcg tcaaagccca ttgtacgcgc gcggaaattc
    52201 atcgcggcta cttcaatgat gattgcaacg tttcgtcctg ttttgacagg gatttttatt
    52261 tgtggaatgc ccacttccgc gatttcaacg gtcgtgtctt cagaacccaa acggtcgtat
    52321 ttcttatctt tcgcccatgc ttctagataa acagctaact ggacttgcat agagcctcgt
    52381 accgcactcg caccgaaaag attcattaca tctatgatcc cgatccctcg aatctcaatc
    52441 agatgttcca ggattttagg aggttctccg attaccgtca gttcatcttg ctgatagaca
    52501 tctactcgat cgtctgcgat caatcgatgt ccgcgcttaa tcagttccaa cgccgtttca
    52561 cttttcccta tcccactgtc cccttgaatt agtacaccta atccgtagac atcgactaac
    52621 acaccgtgga cacttgttcg aggggctaag cgcccatcta aataactaga taattcacct
    52681 agcagccgag acgttgagat aggcgaacgc agcacagcta tcccattttc attggctgct
    52741 tggatcattt cttccggagc cggcaatcct cttgaaataa taaatgcggg catatcttca
    52801 ctacataatc ttctcatgat gatcaaacgc tcttctggga tcatccgttc cgcaaaactg
    52861 atttctttac tgccaaatag ctgtaaacga ttatgagaat aataattaaa ataaccggtc
    52921 aactctagtc ctgggcgtga gatgtcccca gtcacgatga cgcggtctaa acttttctcg
    52981 tcgcctgtga cgatttccaa agataatttt tttactaatt ggctaacttt tacaacttct
    53041 gacattcatg ctacctcatt tctattagag tgttctctac cattttatca ttgtctagcc
    53101 aaaagtacta ccaattaaaa aaaacgtttt tatttaatta ccaatgtaat agaaaacgca
    53161 ttcttaatta tatatgattt gtcaaattaa gctagaataa aatgcttctt gtacatagct
    53221 caaaaatatt tcgattggtg ttctgtaatt caatgatttt cttggaatat aattacgttg
    53281 attgctgaca ctggaaataa atgtctgatt cacttctcta aaatccattg atttcggcag
    53341 tccattacga cgcagaatcc cgttagaatt
//
LOCUS       DACCCR010000010        51804 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCCR010000010 DACCCR010000000
VERSION     DACCCR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.010"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..100)
                     /locus_tag="IVK93_000601"
     CDS             complement(<1..100)
                     /locus_tag="IVK93_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP6385917.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVF"
     gene            complement(115..1362)
                     /locus_tag="IVK93_000602"
     CDS             complement(115..1362)
                     /locus_tag="IVK93_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6385918.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(1438..1584)
                     /locus_tag="IVK93_000603"
     CDS             complement(1438..1584)
                     /locus_tag="IVK93_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP6385919.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1688..1999)
                     /locus_tag="IVK93_000604"
     CDS             complement(1688..1999)
                     /locus_tag="IVK93_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6385920.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(2001..2198)
                     /locus_tag="IVK93_000605"
     CDS             complement(2001..2198)
                     /locus_tag="IVK93_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP6385921.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(2592..4319)
                     /locus_tag="IVK93_000606"
     CDS             complement(2592..4319)
                     /locus_tag="IVK93_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6385922.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(4312..5505)
                     /locus_tag="IVK93_000607"
     CDS             complement(4312..5505)
                     /locus_tag="IVK93_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6385923.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(5692..6336)
                     /locus_tag="IVK93_000608"
     CDS             complement(5692..6336)
                     /locus_tag="IVK93_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6385924.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(6430..6564)
                     /locus_tag="IVK93_000609"
     CDS             complement(6430..6564)
                     /locus_tag="IVK93_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP6385925.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(6566..8698)
                     /gene="feoB"
                     /locus_tag="IVK93_000610"
     CDS             complement(6566..8698)
                     /gene="feoB"
                     /locus_tag="IVK93_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP6385926.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(8695..9168)
                     /locus_tag="IVK93_000611"
     CDS             complement(8695..9168)
                     /locus_tag="IVK93_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP6385927.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            9569..9793
                     /gene="nrdH"
                     /locus_tag="IVK93_000612"
     CDS             9569..9793
                     /gene="nrdH"
                     /locus_tag="IVK93_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP6385928.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9952..12111
                     /gene="nrdE"
                     /locus_tag="IVK93_000613"
     CDS             9952..12111
                     /gene="nrdE"
                     /locus_tag="IVK93_000613"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP6385929.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            12161..13126
                     /gene="nrdF"
                     /locus_tag="IVK93_000614"
     CDS             12161..13126
                     /gene="nrdF"
                     /locus_tag="IVK93_000614"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP6385930.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(13215..14354)
                     /locus_tag="IVK93_000615"
     CDS             complement(13215..14354)
                     /locus_tag="IVK93_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6385931.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(14663..15376)
                     /locus_tag="IVK93_000616"
     CDS             complement(14663..15376)
                     /locus_tag="IVK93_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP6385932.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(15630..16331)
                     /gene="nagB"
                     /locus_tag="IVK93_000617"
     CDS             complement(15630..16331)
                     /gene="nagB"
                     /locus_tag="IVK93_000617"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP6385933.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            16565..18097
                     /locus_tag="IVK93_000618"
     CDS             16565..18097
                     /locus_tag="IVK93_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6385934.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(18288..19490)
                     /locus_tag="IVK93_000619"
     CDS             complement(18288..19490)
                     /locus_tag="IVK93_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6385935.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(19572..20261)
                     /locus_tag="IVK93_000620"
     CDS             complement(19572..20261)
                     /locus_tag="IVK93_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6385936.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(20367..20735)
                     /gene="rplL"
                     /locus_tag="IVK93_000621"
     CDS             complement(20367..20735)
                     /gene="rplL"
                     /locus_tag="IVK93_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP6385937.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(20788..21291)
                     /locus_tag="IVK93_000622"
     CDS             complement(20788..21291)
                     /locus_tag="IVK93_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP6385938.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(21701..22390)
                     /gene="rplA"
                     /locus_tag="IVK93_000623"
     CDS             complement(21701..22390)
                     /gene="rplA"
                     /locus_tag="IVK93_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP6385939.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(22494..22916)
                     /gene="rplK"
                     /locus_tag="IVK93_000624"
     CDS             complement(22494..22916)
                     /gene="rplK"
                     /locus_tag="IVK93_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP6385940.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            23259..23927
                     /gene="sdaAB"
                     /locus_tag="IVK93_000625"
     CDS             23259..23927
                     /gene="sdaAB"
                     /locus_tag="IVK93_000625"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP6385941.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23940..24812
                     /gene="sdaAA"
                     /locus_tag="IVK93_000626"
     CDS             23940..24812
                     /gene="sdaAA"
                     /locus_tag="IVK93_000626"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP6385942.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24903..25922)
                     /locus_tag="IVK93_000627"
     CDS             complement(24903..25922)
                     /locus_tag="IVK93_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6385943.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(26095..26982)
                     /locus_tag="IVK93_000628"
     CDS             complement(26095..26982)
                     /locus_tag="IVK93_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6385944.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(27059..27604)
                     /gene="nusG"
                     /locus_tag="IVK93_000629"
     CDS             complement(27059..27604)
                     /gene="nusG"
                     /locus_tag="IVK93_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP6385945.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(27705..27875)
                     /gene="secE"
                     /locus_tag="IVK93_000630"
     CDS             complement(27705..27875)
                     /gene="secE"
                     /locus_tag="IVK93_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP6385946.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27899..28051)
                     /gene="rpmG"
                     /locus_tag="IVK93_000631"
     CDS             complement(27899..28051)
                     /gene="rpmG"
                     /locus_tag="IVK93_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6385947.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            28213..28857
                     /locus_tag="IVK93_000632"
     CDS             28213..28857
                     /locus_tag="IVK93_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6385948.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(28913..29416)
                     /locus_tag="IVK93_000633"
     CDS             complement(28913..29416)
                     /locus_tag="IVK93_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6385949.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(29429..29475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            30013..30978
                     /locus_tag="IVK93_000634"
     CDS             30013..30978
                     /locus_tag="IVK93_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP6385950.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(31077..32249)
                     /locus_tag="IVK93_000635"
     CDS             complement(31077..32249)
                     /locus_tag="IVK93_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6385951.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(32236..33381)
                     /locus_tag="IVK93_000636"
     CDS             complement(32236..33381)
                     /locus_tag="IVK93_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6385952.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(33395..34319)
                     /locus_tag="IVK93_000637"
                     /pseudo
     CDS             complement(33395..34319)
                     /locus_tag="IVK93_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            34436..35515
                     /locus_tag="IVK93_000638"
     CDS             34436..35515
                     /locus_tag="IVK93_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6385953.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            35547..36140
                     /locus_tag="IVK93_000639"
     CDS             35547..36140
                     /locus_tag="IVK93_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6385954.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(36250..36477)
                     /locus_tag="IVK93_000640"
     CDS             complement(36250..36477)
                     /locus_tag="IVK93_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6385955.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(36580..39168)
                     /locus_tag="IVK93_000641"
     CDS             complement(36580..39168)
                     /locus_tag="IVK93_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP6385956.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(39188..39847)
                     /locus_tag="IVK93_000642"
     CDS             complement(39188..39847)
                     /locus_tag="IVK93_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6385957.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(39896..40399)
                     /gene="trmL"
                     /locus_tag="IVK93_000643"
     CDS             complement(39896..40399)
                     /gene="trmL"
                     /locus_tag="IVK93_000643"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP6385958.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(40620..41486)
                     /locus_tag="IVK93_000644"
     CDS             complement(40620..41486)
                     /locus_tag="IVK93_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6385959.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            41595..42236
                     /locus_tag="IVK93_000645"
     CDS             41595..42236
                     /locus_tag="IVK93_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP6385960.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(42298..43659)
                     /gene="mgtE"
                     /locus_tag="IVK93_000646"
     CDS             complement(42298..43659)
                     /gene="mgtE"
                     /locus_tag="IVK93_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP6385961.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(43677..44576)
                     /locus_tag="IVK93_000647"
     CDS             complement(43677..44576)
                     /locus_tag="IVK93_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6385962.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(44578..45375)
                     /locus_tag="IVK93_000648"
     CDS             complement(44578..45375)
                     /locus_tag="IVK93_000648"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP6385963.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(45353..46057)
                     /locus_tag="IVK93_000649"
     CDS             complement(45353..46057)
                     /locus_tag="IVK93_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP6385964.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            46188..46763
                     /locus_tag="IVK93_000650"
     CDS             46188..46763
                     /locus_tag="IVK93_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP6385965.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            46881..47558
                     /locus_tag="IVK93_000651"
     CDS             46881..47558
                     /locus_tag="IVK93_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6385966.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(47637..49445)
                     /gene="pepF"
                     /locus_tag="IVK93_000652"
     CDS             complement(47637..49445)
                     /gene="pepF"
                     /locus_tag="IVK93_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP6385967.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(49621..50619)
                     /locus_tag="IVK93_000653"
     CDS             complement(49621..50619)
                     /locus_tag="IVK93_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP6385968.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(50845..51498)
                     /locus_tag="IVK93_000654"
     CDS             complement(50845..51498)
                     /locus_tag="IVK93_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP6385969.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15618 a        10778 c         8970 g        16438 t
ORIGIN      
        1 taaatacttt atcgtgaaaa agaatgaaat tatcaataat tcgttctaac tgatttaatt
       61 gtataatttg atcttcacat aatattattg gataactcat aaatcctcct tccctcaaaa
      121 caacaaaata acttgagtag taaattgata cggatcgttt tgatagttca caaacatatt
      181 gggattcttt ttattgattt cctctatact agataaacct aacccttgat gtcccttttt
      241 tgttgttatt ccttttttca ttagagtatc tatagagagt agtatctctc tacaactatt
      301 cgatatcgaa atttctatct gtttatcatc tcggtaaata gctagagaca gttctctatc
      361 ttgactctct atagatgctt caattgcatt atcgagaata attccaagca ccctaataca
      421 gtcaaatatt ggaacaggaa cttcaataat agtacttgga cattcgaatt tacaacaaat
      481 cttattgtta tttgcttggt atagcttcgc tatcaacaaa cttttcaaat attcattttt
      541 tatatttttt aaataacgat attcaaactt attggttaga taagaattag aatagatctc
      601 taattctttt atttcttttt ccaatatcga atcatggttt tgattagcga tttcttttaa
      661 actcagcaat aaatttttat agtcatgtct aaatctggca aatttttctt gatctttttc
      721 cagtgaatca gtatattttt ttagataaac caattctttt cttttcaatt gctgttcata
      781 ttgttctcgt tgcctaatag ttattctaat aaataaaaat agaacaacaa acatttgaat
      841 gattaaaaat attaagacac caaacacaaa atgatcaaat acttgatatt gatgagcaac
      901 ataagaaaca aataaaccaa ttgtaagtaa ataaatcatg aatatagcag taagtttaga
      961 actggactgt tcaacaatcc tatttatagc taattttcta tataaccaaa taaacatcag
     1021 caaaaacaaa gaatcaagca ttatctggaa tactacataa ccataccctt ttatcccttc
     1081 aaatgaaaac atatacgtca taaccatgga agtaataata gatctgaaat catcaataag
     1141 catacataat agtaatttat ttaaaagcac tatattagtt ttcttctttt cctttaatat
     1201 aaaataactt ccgattatag gtatagctgc ggaaacatca gtaaataatt caacaaaaaa
     1261 agtgagtggt atcaataaac atacaaaaag atattttctt ttgttaagat cagtattagt
     1321 catataaaat aagagaaaaa agaaaaagtt tcccacgaac attcttaata gataagcttt
     1381 tgtatcgata gtaatcaaga gatcccctcc tgattttagg atatcataaa aaaaatttta
     1441 gttacacttt ttaaataatg aaaaaacaca ttccactaaa tttgatgccg gttttccttg
     1501 aggttttgtt ccagcacctc ctttaatttt tttaagctct tgatctgaac attgtttagc
     1561 atttagtcta tttttttctt ccattacgat tccccctaca aactataaat acttttatgt
     1621 cactgattca atatacctat ttttctaatc aaaaaatttt gagacatatt taatattata
     1681 agtatcatta aaattgagat ttatctccat aatctgctcg atataaacca tttattccag
     1741 cagttcttcc aatttcactc aaatttctta ttaattcctc ttgatattca gtgaatctta
     1801 ttttattagt ataagcagta aaatatagat aattcaccaa tctatttatt aaaggaccgg
     1861 gatctaattc atatttttgt ttttctaatt ttaaatatac cttttgtaaa acctcaagaa
     1921 tatcagaata tttaggatct ttacttatag atatatcatt atataattca tgaacaattt
     1981 gcttagcatt ttttttcatt ttagcacttc cctggaattg ctccacctaa aaaaccacct
     2041 atagacattc ctgcaataca agtagttgcc ttggcccaat cgaccgtaca tttattttta
     2101 gtgcaataca ctccatttcc ataatatttt ccactatgag tggtaccccc atagataagt
     2161 tgtgtctctt taatagacaa aatttttaaa tgtttcataa tctaatcacc cctaaattaa
     2221 tgtttttaga ctattattct aacattcatt aaatttcaaa aaacaattcc ttcttgaaac
     2281 gtagcaaaat aattcctgaa taagaattta atcactttta agtatataaa ctatgtatat
     2341 tctttaatat tttttgagaa tgcacttcgt acataataaa ctttaatcac attagtcttt
     2401 catacctacg atcgatctct ttgactcgca cccataaatt cataatagtt tgtcggcgtc
     2461 tttggacgtt tcgaagatga gctacgcatt ctcttttttt atagacaaaa aagatagaga
     2521 cgaacatgga gcttccaaat ttggatattt gatccaattt tggaagctcc atttatttcg
     2581 tgtctgtctt tttatttttt ccagctttcg actaattctt ggttctcttt gatccagtct
     2641 tttgctgctt gctgtggatc ttttccattg ttgatttcca acataacttt ttccatatct
     2701 ttttctgacc aatggaaatt gtctaatact ttgtaagctt ctggctggtc ttctttcaat
     2761 ccttttcgaa ccatcgtatg gatctgctct tcgcttccca ttccgttttc aggatcttct
     2821 aagtatttca gatcatattt ggcaaacatc caatgaggtg tccatcctgt aactacaatt
     2881 ggttcgtgtt ttttgatcgc ttgacctagt gcaacggtca ttgctcctga agaagatgtc
     2941 tctactttcc agtctttcaa gttgtcatat ttttggatcg tgttttcagc agccgtaact
     3001 acaccagctc ctggctcgat tcctatgatt ttcttacctg cctgatcggt taactcatcg
     3061 atcgaattta catccatgta ggctggcact gctaaaccaa cttttgctcc tgtcaaattg
     3121 gcacctaggt cttcgacttg atctttatat tgtgcatatt gtgaaccatg agttttaggt
     3181 agccaagctg aaaccatggc atctgactcg ccatttgaaa cagatttcca catgattgaa
     3241 ttatctaatg gcgtcatcgt tacatcatag cccatttgtt tcaatacttc accgactaca
     3301 tttgttgaag caacttctgt atcccattca acataagata agttgatacg ttttgtttct
     3361 ttggcagatg aaaaactccc tataccaatc gcacctaaga tcactactac tacagcacca
     3421 atgatcagtc cttgacgttt tttacttttc tgtgttgttg ctcctgcagc ttttttattg
     3481 tttggctggt tcaaatgctg tgtaaaacgg tcaatgatga ttgctaagat aactaatgca
     3541 accccgttga caaatccatt cccgacttgt gctctttgca atgctgataa tacgccgcgt
     3601 cctaagccgg gtgcaccgat catagaggca ataacgacca ttgaaagtga cagcattgtt
     3661 gtttgattga tccctgccat gatcgtactt ttagctaaag gcaattctag tttgaatagt
     3721 ttttgacgac ttgtgctccc gaatgaatca gaagcttcta ctaattcttt aggaacttgt
     3781 cgaatcccta aattcgtaaa acgaacggtt ggtggcagag caaagatgac tgaggcaaat
     3841 acccctggaa ccatcccgat accgaaaaat gcaactgctg gaatcaaata aacgaatcct
     3901 ggcattgtct gcataaagtc aaggatcggt ttgataatac tttgggcttt actgcttttt
     3961 gccatcaaga tccctaatgg taccccaatg atgatcgaga tcacactaga taaaagaaca
     4021 agtgtgacag tattcatcaa atcattccat agaccttggt tataaataaa ccatagaccg
     4081 attaatgtga acaacgtaag tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc
     4141 aataacaaga taaacaaagg agctggaatc actaacagta aatttgtaat ccccgacatc
     4201 actgcctgtc caatcgtttg taaaaatgaa aataggccag aaaaagttgt cgtaaaccat
     4261 tctgtgatat tttctaccca gtttgccact ggtaattgat agttcaatag attattcatg
     4321 ttcactcacc tcgcttgttt ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc
     4381 ttttaatttt ccatcctcta caactgcaag tggagtcggt gagtcataaa taatattgaa
     4441 aatatcactt accaccatgt ctttcccaat caccgtcaca ttttcatcta ggaagtcgat
     4501 caatggacgt ttttgcttcc tagcttctaa cgcttgatct gcagtgatga tccctttcag
     4561 gtgtctcttt ctgtcaacag ccatcagcat gctgacttct tcattacgca tccttgtcaa
     4621 tgcaacgttt ggtccatcag attcgatatt cgtcgtcaat gcaggtacca tgatgttttg
     4681 agccgtaagc actttggaac gatcgacttc ttccacaaat tcacgtacat aatcatttgc
     4741 tggattggtc aatatttctt cacctgttcc gatctgcatg atttcgccgt ctttcatcaa
     4801 ggcgattctg tccccaatcc gtaaagcttc attcaaatca tgggtaatga aaataatcgt
     4861 tttttgaaca ttggcttgca ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg
     4921 atcaagagcc gagaacgctt cgtccatcag caagatctca ggatcatttg ctaaagctct
     4981 ggctaggcca acccgttgtt gcatcccacc agataactga ctaggatatt ggtctttaaa
     5041 tgaaagcaaa ctagaatttt ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg
     5101 tacaccacgg acttctaacc cgtattctgt attttccaag atcgttcgat gtggaaacag
     5161 accgaagttt tggaaaacca tattgatttt atgacgacgg acttctcgca attcttcttt
     5221 gcttaacttc gatacatctt ctccatcaat atagatactg cccgaagttg gctcaatcag
     5281 acgattcaat agacggatca atgtggattt cccactcccg gaaagcccca tgatcacgaa
     5341 gatttctcct tcttttacgt caaaattcac gtcatacaca ccaaccgttg caccggtttt
     5401 ctttaaaatc tccgttttcg gctggtgatc attcatcata tctaaagcgg cttgtgtctt
     5461 tttaccaaat atctttgtta aatgttttac ttctactttt accaaacaaa ttctcctttt
     5521 ttatgtaaat tcatttttct ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt
     5581 cactttgtca aaaatgaaag aaatttgctt gtccaaactg aaaaaaactt ggctaaatct
     5641 ttgtaaataa aaggtttagc caagttaata tttttttaat atttcacaca atcattctgc
     5701 aagctgatgc tccgggtaaa aaaagtttgt catacgctgt agtgtatatt catttccaat
     5761 aaaatgaacg atgtcccctg ccgtaaattt agcatagggg ccaggtgaaa caagcagttc
     5821 ttcattatga gaaattgcga taactgtggc gccagtcgcc tgccagatat tcaactcgct
     5881 aattgaacgg tccagatgca ttgctttttc tgataaagat aattcaaacg gaagtaaagg
     5941 attcatctta tgtattcgct tcgtttgatt gaccagctga tccaataatt gagaaaaatg
     6001 attcagctca tcttgttgct tctccacact tgccatgatt tctgcttttg tatcttggat
     6061 ggattgtaca tcttgatatt gcaataggaa atcttttgct ttttccttcg aagcaacaaa
     6121 tgccccactt ccatgccgta cttccatgat tccaagatct accagcacat tgatagcctt
     6181 gcgggtagtc tcaggagaag cattgtacgt actggcaagt gttgagcggg catggatctt
     6241 ctcacccact ttatatcggt tttcaacgat acgttcggct aaatcaacag caatctgctg
     6301 gtagcgcggg cggacgattt ttttctttcc agacattttc tttcctcttt ctttttttac
     6361 tttccctcat cttactatgc tctagcaaaa taagcaaaaa aggggagaat agcctcttgc
     6421 aacgactgat catcgcttga tctgttcctt ttttactggg caagctgttt gacagtcatc
     6481 acaattctta ccggatttta aacgagtata gacaattgcg cctgcacttc caaagattaa
     6541 cacacttaat aagaatgtcg ccatcctatt cctctccttc taatgacaat atagtataaa
     6601 actggttctt ggattttggc tgacggaaca aaacataaac gagtcctatc atcagtaaaa
     6661 tagcaagata cgtccctaac gctacctgtc caccctccaa taagtgtccc aactgataaa
     6721 tcacaaaact aactaaataa gctaaaccgc attgatagcc tatggcgatc caagtccatt
     6781 tcttggcatt catttcccga tggatagccc caattgccgc aaaacaaggc gcacaaagta
     6841 aattgaaggc taacaatgaa taagctgctg cgggagtata tgtgtgttgt agcacttgcc
     6901 agatttctct gccattttcg gatacttccg catgaccaaa caaaatacca aatgtgccaa
     6961 taatattttc tttagcgacc aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc
     7021 aaccaagcgg ctgaaaaaca actgccagga ttcgcccgaa aaaggctaag atactttggt
     7081 cttcagaaac aggttgagca taaaaattat aagaagagct gaaccagatc acgatactag
     7141 aaacaaagat aatcgttcct gcttttttaa caaaagactt gctgcggtca taggtttgtc
     7201 ttaggaccgc tgaccattga ggaaaatgat atgcgggcaa ttccatgata aaaggagctg
     7261 tatctccagc aaacaaacgt gtttttttca agctgattcc agagaaaata attgcagtca
     7321 taccaagaaa ataggcagaa ggagctaccc aagattgatg aggaaaaaag gcccctgcta
     7381 ctaaggcaat aatcggcaac tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca
     7441 aacgacgatc ttgctcgttt tcaatcgtgc gactggccat gacgcctggc actccacatc
     7501 ctgtagcaat cagcatagga atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc
     7561 gatccatgac aaaagcgatc ctcgccatat atccgcagtc ttccagaaag ctcaagcata
     7621 aaaacaatat aatcaattgc ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc
     7681 cgttcaagat cagctcctgc atccatcccg ctacttgcca ttcagctaac aatctgccaa
     7741 catgttctgg taaccatgta ccaaacaatg tgtcattgat ccaatctgtc cccattgttc
     7801 ctactgtttg aatagccaga taatagatca gccacatcac aaatgcgaag atcggcaagg
     7861 ctaaccaacg attcgttgca atctgatcga ttttgtcact catcgataat tggaaagaat
     7921 catttgacac aacacagagt gtacatagtt gggtaataaa ttcataacgt tcattgacca
     7981 gaatcgtctc actatcgtcg tccaatagtt tctctgttaa ctggatcagt tgctcgatct
     8041 ctttttgctg agaaactgct atctcgtaat cctctcttac cttctgatct cgttcaaaat
     8101 atttcattgc ataccagcgt ttcttgccgc tttgttctcc tagtacatct tcaatttcat
     8161 gtaaagctgc ttctaaacgt ggatcatatg tacagggaac aggaatagaa ggccgctttt
     8221 ttgcagactc ctgaatcttt tcgattagtt tttcgattcc ttgctttttc aatacgctga
     8281 ttggtaaaac tggtacacca agaccatagg aaagcttttc tagattgatt ttttttcctt
     8341 cttttttgat gacatccatc atattcagac caatgatcat aggaatgtct aaatccaata
     8401 actgcgtagt taaataaaga tttcgttcta aattggtagc atcaataatg ttgatgatca
     8461 gttcaggctg ttcctgaacc aaataatctc ttgcaatcac ttcttctggt gtataaggag
     8521 acaaagaata gatgccgggt aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc
     8581 cttctttttt ttctactgtt acgcccggcc aattccctac atattgattc gaaccagtca
     8641 agagattgaa caagctagtt ttcccgctgt ttggatttcc gactaacgcg atattcattc
     8701 tttttcctcc ctttgtatca agattagttc ggcctctgac tttcgtaacg tcagttcata
     8761 ccctcgcact gtcagttcga ttggatctcc cagaggggct agttttcgga taactatttt
     8821 tacatttcgc gtcaatccca tatccattaa ccgtcgcttc actgcgcctt gtccgtggat
     8881 tgctacgacg atcccagtct ctcctgcttt taattgatca agagaagtcc agctttcaga
     8941 ggaagcagtc tttttattga caaagatttg acctagtatt tcccgactta ctgccactcg
     9001 atgacttttt accagtaaaa tggcattgtt gcctgataaa ttgacgacga cgattttact
     9061 tccggcaacg atcccgatat tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg
     9121 ttcgacttga taaacgaaat tctgctttgc ctgcgctaag gtctgcatat tgattcctct
     9181 cttgtgaaat aaatcatgta ttaaaatcat tcattatatg ttctaatttt cttacgtgtt
     9241 acgcaaataa atgagaatca ttctcacata gaatactctc ggtaattgag aatgtcaatt
     9301 atacttgtga tattatgagc gaatctttag tattgataga taaaaatgag aaaaaaatat
     9361 ttcttcatag ttttccacaa ttttttctct attaaatttg ctattttccc acacgatact
     9421 tgttttcagc tcttttttca aaaacacaaa acttagtatt ccacagtttc cacaacacaa
     9481 tatgtagtgt ttgttgtttg acttttttac taagagtttt atactatcta ttatagataa
     9541 taagaatgtg ttagaggagg cactcaccat gaacgtaaaa atcttttcta aaaataattg
     9601 tatgcaatgt aagatggtca aacggttttt aacagaaaat caaattgctt ttgaagaagt
     9661 caatatcgat gaacagcctg aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt
     9721 tcctgtcatt acttctgatg cagaaacagt tatcggattc cgtcctgatc agttgcgtaa
     9781 acttgccagc taacatgact aaaagaatca aaacgtgata catggatttg aagggcttga
     9841 gacagcacca gtttttgtcg caaacttcca aatccttttt taaagcttaa aaacaatgca
     9901 atgatttgca atcaagcatc atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt
     9961 aaaaatttac aagatgtcag ttatttcaag ctgaataatg aaatcaatcg gccagtcaat
    10021 ggtcaaatcc ctttgaataa ggataaagaa gctttagttg ccttttttga ggaaaatgtc
    10081 aaaccaaata cgatgacttt tctatctatt atggaaaaaa tcaattactt gatcgatcaa
    10141 gaatatatag aagcagaatt cattcaattg tattcaccat catttattga aaagctatat
    10201 caatttttgg acgagcaaca tttttctttc aaatctttta tggctgctta caaattttat
    10261 tctcaatatg cgttgaaaac caacgacggt tccgcttatc tggaaactta tgaagatcga
    10321 gtagccttca atgcgcttta ctttgcgaac ggggatgaag aattagcact tgctcttgca
    10381 gacgaaatga tccatcaacg atatcagcca gctacacctt ctttcttgaa tgctggacgt
    10441 aaacgtcggg gcgaattggt gtcttgtttc ttgactcaag tcactgacga tatgaactct
    10501 atcgggcgct caatcaactc tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt
    10561 actttatcaa atcttcgtga agccggcgct cctatcaaag gatatgaggg tgctgcaagt
    10621 ggtgtcgttc cagtcatgaa attattcgaa gatagtttca gctattctaa tcaattaggt
    10681 cagcgtcaag gagctggcgt ggtttatttg aacgttttcc atccagacat tattgctttc
    10741 ctttcagcaa aaaaagaaaa tgcagatgaa aagattcgcg taaaaactct ttctcttggc
    10801 gtgatcgtac ctgataaatt ttatgaattg acacgtaaaa acgaagatat gtatttattt
    10861 agtccatata gtgtcgaaaa agaatatggt gtcccattct cttatgtaaa tattacagct
    10921 gagtatgatc atctagttgc aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat
    10981 cttgaaaatg aaatctcaaa attacaacaa gaatctggct atccttacgt aatcaatatt
    11041 gatacagcga accgcgagaa tccaatcgat ggcaagatca tcatgagtaa tttatgttca
    11101 gagattttac aagttcaaac gccttctgtg attaacggca aacaggaata tgaagtatta
    11161 ggaacggata tctcatgtaa cttaggctcg acaaatatcg tgaatttgat gcaaagccct
    11221 aactttggtc aatctgtgcg tacgatgact cgtgcattga catttattac agatgcatct
    11281 gatatcgatg tcgtaccacc gatccaaaac ggaaataagc tgaatcatac gattggatta
    11341 ggtgccatgg gcttacacac ttatcttgca aaagaacaaa tggaatatgg ctcagaggat
    11401 tcattagact ttacaaatat ttacttcatg ctgttgaatt attggacact tgttgaaagc
    11461 aacaatatcg ctcgtgaaag aaaacaagtt ttccataatt ttgaaaaatc agcttatgct
    11521 gacggcactt atttcgaaaa atacgtaact ggagaattcc agccaaaatc agaaaaagtc
    11581 aaagcattgt ttgaaggcat ttttatccca actgctgaag attggaatgc tttgaaacaa
    11641 gcagtaatga aagatggact gtatcatcaa aatcgcttgg cagtagcacc aaatggctca
    11701 atttcatata tcaacgatac gagtgcaagt attcatccta tcactcgcat gatcgaagaa
    11761 cgccaagaga agaaaatcgg taaaatttat tacccagcgc cttatctatc caatgagacg
    11821 attccgtatt acacttctgc ttatgatatg gacatgcgta aagtaatcga tgtttatgca
    11881 atggcacaac aacacgtcga tcaaggcatg agtttgactt tattcatgcg ttctgaaatc
    11941 ccagaaggca tttatgaatg gaagacaaca acgaagcaaa cgacacgtga tttgaatatt
    12001 ttaagacatt acgctttcca caaaggaatc aaatccattt attacgtacg tacctttact
    12061 gatgatgcag aagaaatcgg aagcaatcaa tgtgaaagct gcgtgatcta atatcataga
    12121 tagattcgct tattcatatt ttaattaaag gagcattctc atgtcagaaa catattatga
    12181 agcaataaac tggaatgaaa ttgaagatat cattgataaa tccacttggg agaagctgac
    12241 agagcaattt tggctagata cacgtatccc actttcaaat gacctagatg attggcgtac
    12301 attgtctcaa ttggaaaaag atacagtagg acatgtattt ggcggtttga cactgctaga
    12361 tactgtccaa tctgaaagtg gcatggatca gttgcgtaaa gatgtccgca ctccccatga
    12421 agaagccgtt ttgaacaata ttcaattcat ggaatccgtt catgccaaaa gttattcttc
    12481 tatttttagt acactgaata cgaaaaaaga aatcgaagaa atttttgact ggacaaatac
    12541 gaatccttat ctgcaaaaaa aagctgagcg tatcaatgaa atctacaaac acggtacacc
    12601 gctagaaaag aaaattgcca gcgtcttttt agaaacattc ttgttctatt caggttttta
    12661 tactcctctt tattatttag gaaacaacaa attggctaac gtagcagaga tcatcaaact
    12721 aatcatccga gatgaatctg tacatggaac ctacatcggt tacaaattcc aattaggatt
    12781 caacgagtta cctgaagcag aacaagaaac gttaaaagac tggatgtaca atcttcttta
    12841 tgagctttac gaaaacgaag aacgttatac agaagaatta tacgatccaa tcggctggac
    12901 agaagaagtc aagacattcc tacgctataa tgccaacaaa gcattgatga acctagggat
    12961 ggatccactc tttccagata ctgccaatga tgtcaaccca atcgtaatga acgggatttc
    13021 aacaggcaca agcaaccatg acttcttctc acaagtcgga aacggctatt tgttaggtca
    13081 tgtagaagcg atgaaagacg acgattattt gattggattg gaataaggat ttattttaat
    13141 tcaaaaattt ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa tgtacactga
    13201 actctttttt atgcttattc acttgtttct tccagcactt ttggactgtc taacttttcc
    13261 gaaacatgtt ccattcttct caataaatag attttccgga tatttaatat tatcgtctta
    13321 atcactgcat agacaggtat gcaaatcagc atgcctaata ttcctgcgac acttcctgct
    13381 ccaatcaata ccaagatgat cgtcaaagga tggatattca ttgatttccc aaataacaac
    13441 ggcttgatca aatttccatc taattgttgg atcactaaaa tcgacaacgc catatacaaa
    13501 gcttgaaatg gagagataaa caaaccgaca atgattgctg gtaccgctcc aataaaaggg
    13561 ccgacatacg ggataatatt cgtgattccg caaatgaccg caagcaacaa gctataaggc
    13621 tgatcgaata ttctcattgc caaaaagctc attacaccaa taatcaaagc atctaagatc
    13681 gtactgctga tataggcaga taatgtttcg tttaattctt ttaccgtttg gcgtaactct
    13741 ttacgaatcg ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg cccatccttg
    13801 aacataaata gcaaaataaa tggaacggta aataacaaaa taaaaaattg catcataaat
    13861 gaaatgattt ttgagacact tgcagataaa ctgactaata tgatattgaa caaattacta
    13921 agagataaat ttgctgatgc taattgttct tccaaattga aatttttaaa ctcttcccgc
    13981 tgcgccaatt catttaacca tttgcttgtt tcatctgcat acattggcaa gctctgggtc
    14041 aattgaattg tctgttccat taactgaggc actacattca tgacagttaa aacaactaaa
    14101 atgattacga ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg atgcttttcg
    14161 agaaaaacaa cgattggatc gaacatataa tagataaaac cagcaacaag caatggtaaa
    14221 aaaactgccg aaagcatttt caatattgga ctaaaaatat tcggcatttg taaaataaag
    14281 tacacgccga tgatcgtgag tactagctcc actgtccaga aaaataattt tgattgttta
    14341 aaacgtgatt gcatacatct cctccaaggt cctttgatac ttctattcta tcatttaaca
    14401 aagagttacg cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata aacatatttt
    14461 aatgagtaga cgagtactta aacaaaaaca cggtttggag tagtgacaaa aattatactg
    14521 cagtcccttt ttccaaataa acggtgttca aaagcttgca tcttcgtaat aagcccagcc
    14581 aaacgaaaaa tataaggaac tatttttatt tggctgggct tattacttga tgtaggacat
    14641 ctattttaca acctcagatc atttattgcg caatactgac taaatgcaac tttgtgatat
    14701 ttttgtgtac ggacaactcc tcgatcacat cggcataagt gagccctttt ggaagatcaa
    14761 tcgtatagat atttttgtac actcggtagt catcaacgaa ttttacgtca aaattcacat
    14821 cttcgattac gatatttttc tcttcaaaat aatcggtaag aaaatctttg gtttcttttc
    14881 tatggataaa ttggatttct aactttttag tcgtaggtac atggatcacg cgtttcacta
    14941 ctgtgagtgc aatagcaatc ccgataaaac tgatgatcgc aatggaatag taacccatgc
    15001 cgatggcgat ccctaatcca gcaattgccc ataaagaagc ggcagtcgta agtcccgtca
    15061 ccgattgttt cgtgacgatg atcgttcccg cgcctaagaa accgacacca ctcaccactt
    15121 gagcaatcag ccttgcttca tctgaacgaa tcgttccagc aagctgtgga tactctaaag
    15181 catctcttaa tgcaccacta gcgatctcaa cttggatcaa tgcaatgata catgctccca
    15241 tacaaactaa aatatgcgta cgcatacctg ctggacgatt tttatattgt cgttcaaaac
    15301 cgattgctcc gccaaatagc atagcgatcc ctaaacgtaa aataatctct ggaattgtca
    15361 ataccatatc attcatctaa acaccctcca gtccaatata tcatgtctga aaggagcaga
    15421 caaaaaaagt gcaattttta cctcttacga aaaaaggact gtgacaaaat ttttgtcata
    15481 gtccttttta tgaataaacg gtgttcaatc agctgaccct cggtattact caacaaacgg
    15541 caaaatatgg ggagctattt ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt
    15601 tcacaacctc tttagcctac tattgaaaat tacaacttac ttgctgcttc ttcatcgatg
    15661 atcacaacaa cattgtcgtg attttgcaat acacttgctg gcaaatgatt cgttacaggt
    15721 ccttcaatca tccctttgat tgcatcagct ttgctttcgc caaatgccat caagacgatt
    15781 tctttacttt tcataattga accgattccc attgaaacag cttttgttgg tacatcttct
    15841 gctttatcga agaatcgttt gtttgcattg attgttgatt ctgttaagtc aactactgaa
    15901 gtcgttccgt caaatgaagc accaggttca ttgaaaccga tatgtccatt ttgcccgatt
    15961 cctaagattt ggatatcgat tggatgtgca tcgatgattt gatcatactt tttgcatgct
    16021 gcttccagat cttctgcttt tccatctggt acaaacgttt ctttgaatgg ttttttatca
    16081 aataattgga catccatgaa atgacggtag ctttgttcat ctgaaccgcc tagtccaacg
    16141 tactcatcta agtttacaga agtcatttca ctgaaatcca aatcgcttga agtcatttct
    16201 ttatataaag taactggtgt gcttcctgtt gccaatccta atacttttgc acctttgttc
    16261 ataccttctt tgatgatttc aaatgctttt tttccgcctt cttcagcgtt ttttacacga
    16321 atgatttcca tagtgattgc tcctttatat tttggtatag cccaattata gcattctttt
    16381 tttcttttta caataattag tatagaccaa attgcatttt tttcattttc ttgacaataa
    16441 ataggaatcc gtgttaggtt tcataagatg gttcaatcaa acaatccatg ttgcatgaaa
    16501 accaagcaag tgattcactg tggacgcttt atgtctgcag tttttttatg aaaaaggagt
    16561 tattatgagt atactgacat taaacaacat tacccagcaa tttggcgaca aaatcctcta
    16621 tgaaggtgtc gatctgcaat taaatgctgg cgaacatctt gggctgattg gacaaaacgg
    16681 agccggtaaa agtacattga ttaaaatcat cactggtgag atcttaccag atgacggaca
    16741 gatccattgg caaaaaaata ttcacaaagg ttacctcgat caatatgtgg aagtcgatga
    16801 aacattaaca atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga
    16861 aaaaattgct tttctttacc aaacatatag cgagaccttc gatgattcct tgctggaaaa
    16921 agctgggaaa ctccaaacgg aattagacca aggagtgttt tatcaaatcg acaccttagt
    16981 tgctgagatg agcagcgggt tggggatcga tgtcttaggt ttagattcac tgttgaaaaa
    17041 tctaagtggt ggacaacgtt caaaggtgat tttagcaaaa ctacttttag aaaacccaga
    17101 tgttctgatc ttagatgaac caaccaatca cttagatgat cagcatatcc aatggctgac
    17161 gcagtttctt caggattttg acggtgcgta tatcgtgatt tcccatgacc aagaatttct
    17221 tgatcagatc accacccata tcgcagacat cgagtttgga aagattacta agtatactgg
    17281 ccatctaaaa caagcattga aacaaaagga acaaaatcgc gaaagctatt tgcgccaata
    17341 tcatgctcaa caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat acaaagccgg
    17401 ttcccgatca acgatggcaa aaagccgaca aaaacaatta gataaaatcg aacggctgac
    17461 gcctccagct tcagcaagta aagctgtctt tgattttcca ttctcgccta ttgtcacgac
    17521 attagcagtg gaaacaactg atttagtcat cggatatgat cgaccgctac ttgcacccat
    17581 caatttgacc atccgctttg gtgaaaatat tgccattcga ggcttcaacg ggattggaaa
    17641 atctacttta ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg atttccactt
    17701 cccagataac acaaaaatca attacttttc acaagatctg tactgggaaa atccattaga
    17761 aacaccacta caatatttaa gcagtcaatt tccaaaagcg actatcaaag aattacgtcg
    17821 ccaactagct aaagcagggt tagttaatca attagcaagt gaacctctca ctacattaag
    17881 tggtggggaa caaactaagg tgaaacttgc acagttgaca atgaaccaag gtaatttact
    17941 gattctcgat gaaccgacga accatatcga tcaagaaacg aaagaaagct tacaagacgg
    18001 gatacagaaa tttccaggaa cagtaatcat cgtctctcat gaacaagaat tttatcaaga
    18061 tcttgtgcaa cgagtcattg agattgaacc aaaataaacg tttgattgaa tagttgaaaa
    18121 aaaggagctg tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg gtggccaatt
    18181 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggagctatt tttatttggc
    18241 tattcttatt acttgatgca gaacagcttt tcacaacctc ttttcgtcta ttttttattt
    18301 tcgagatttc tctttccttt aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa
    18361 acaagcccgc taaccgttac acctaaccag ccatactgct gccacattgt cgtccctagc
    18421 aatgaaccag ccgcacctcc aataaagtac ataaacataa atacagtatt atttcggttg
    18481 cttgctgctt cacctagccc ttgtactcgt gtttgattgg cgacttgacc aaattgattc
    18541 ccgacgtcta ataaaataat agctaaaagc aaaatggcca aatggcttcc gccaaccaat
    18601 aaagcaataa agcttattgt ttgcataaca agtcccatat acacgatttt tctttcagaa
    18661 taagaatctg ccagtcgtcc cacgataggt gcagcaactg ctccagacaa accaaaaatc
    18721 gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc
    18781 aacgtagacc aaaagatcga aaatgttcca aacatgaaaa atccactgac tgccgcttct
    18841 cttaatagac gttgagaagc aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa
    18901 acagctgtac tactttgctg gacagaacgc ggcattttcc agtaaagcag aagtgtaaga
    18961 attccgactg cacataaagc aaataaatag atcatccgcc aagaaaaaag actggcaaca
    19021 ataccagata ccgtccgaga aagcaaaatc cccacgagta aaccgctaag caatgtaccc
    19081 atgaccttcc ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat gatgatttgc
    19141 ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc
    19201 gcaaaaaaag cggccaacag agagacggca gataacgctg ccattcgaat gatcaatttt
    19261 ggacgatcca ccacgtcccc taaaggaact aaaaacagta accctaacgc atagcctaat
    19321 tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa cagataaact agctgctact
    19381 tttgttccaa tcggctggat gtaatacata ttggcaacaa caacgccaca agtgattgat
    19441 aatagtaaaa taagcgaagt tgttaaaggt tttttttcat tactcttcat tgatttcccc
    19501 catttcctat cttagtttac aacttatgga aaagattgga aatagaaaaa tacaactatt
    19561 cgccacactt attatttgtg cttttgtttt ccttgttttt tcttgcgatc tttataaatg
    19621 aagacaacta accctactag cacagtaaca cttgcaacca acgtgactgc agttttgtta
    19681 tatccgtgaa acagagcttc tccaccacac gcatacaaca cagacgaagg gatgactcca
    19741 atcacgatcg acaatatgat ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg
    19801 accatagaag atggaataac cggtatcata tagcctaaca ttacacctat ttttggatgt
    19861 ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg ttttcttatc taaaaatttt
    19921 aagcgatgca taaggaaaat tgcagtgatg ttaccaagag cattacccaa aatgttgatg
    19981 atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga ctccaactac tgaagtagga
    20041 atacctggaa cagcacacat caccgctgtc aatacgatca agagtatcga tgcaaaagtc
    20101 ccatgcgaac gaatatgtgc gagtagctct gccttactcg ctctcggatt taaaaataat
    20161 tggatgtctg tctgatattc taaatacaac cgatagccca ctaccagtat cagaatgatt
    20221 cccaacgaga tcaatatcaa tcggattatt ttctttttca tatttcctcc tgaaaaaaat
    20281 ctatattcat taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg
    20341 atctcagtgc ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca
    20401 agtttagctt tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga
    20461 gcgccatcaa ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct
    20521 ttgataactt taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt
    20581 tcttcagcag cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg
    20641 ccaaattctt cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt
    20701 aactcagcaa cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc
    20761 ttttttattt aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac
    20821 tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga
    20881 tagaagtcct tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac
    20941 tttaccttcg atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc
    21001 catgattttc gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt
    21061 gaatacttcg tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt
    21121 tttgataact ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc
    21181 atcaactgtt aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc
    21241 gcttacttca tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt
    21301 tcacctcctg aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag
    21361 ttactttgct caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc
    21421 gctttgctcg aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc
    21481 acttcgttca tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta
    21541 ttgaatatat tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct
    21601 tcggtacaag ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga
    21661 attatttttc agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa
    21721 catggatacc aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag
    21781 ctgctgatgg tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt
    21841 tttcatcgtc aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac
    21901 ggtaagttac tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg
    21961 taccagtttt agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta
    22021 cagtagccat catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga
    22081 ttttttgaac catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg
    22141 ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata
    22201 caacagcacc acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa
    22261 cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg
    22321 caacaggata aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct
    22381 ttttagccat tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc
    22441 tatatctttc aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct
    22501 acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta
    22561 gctgcgttta ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact
    22621 ttcgcaactt tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc
    22681 aatagaacag cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca
    22741 gaaataacaa ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt
    22801 gtgaatccca tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga
    22861 gttgctttac ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga
    22921 catacctcct taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt
    22981 tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat
    23041 agcaaattta tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa
    23101 acgctaaatt tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac
    23161 acttgcgcta gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt
    23221 gtacaaagat tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa
    23281 gtgtctttga tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag
    23341 ctgctagaat cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata
    23401 tttatctata tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag
    23461 ttggcggcct gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg
    23521 catatgaagc cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa
    23581 actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga
    23641 tcggcggtgg caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg
    23701 gtactcctac cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta
    23761 gtatactttc tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag
    23821 gtgaaaatgc cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa
    23881 gactcaaaca gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa
    23941 tgtttttatc gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat
    24001 tgatgattgc agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca
    24061 tggaaaaaaa cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct
    24121 ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag
    24181 actttttaag tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag
    24241 tcaatgcaaa aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc
    24301 ctggtgtgct gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat
    24361 tcttatttac cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg
    24421 cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg
    24481 tttgtgcaaa aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa
    24541 atatgatggg actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac
    24601 gtaatgcttt aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg
    24661 aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc
    24721 cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac
    24781 ttgcggaaga gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga
    24841 gattagttat cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt
    24901 tattaatcca tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc
    24961 atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca
    25021 cctagattca acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc
    25081 agagctttga agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt
    25141 ttgttgacta cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctagt
    25201 tgacgtccac catgattgga tacccagata ccatctgccc ctgcagagat agcgattact
    25261 gcatcttctg gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt
    25321 gttttgatgt catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct
    25381 tttccttcgc cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt
    25441 aatggaaatt ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct
    25501 gccgttaaaa tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg
    25561 aatctgtcgt ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg
    25621 gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca
    25681 gctactcctt tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat
    25741 ggcgcttgga taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct
    25801 gagtcgattc cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt
    25861 tctttcatcg tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc
    25921 atgcgctctt ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct
    25981 tcattacttg tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa
    26041 tcatactaaa cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc
    26101 tttccaaata aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg
    26161 agaggcattt tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa
    26221 cgcatagacc gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc
    26281 tagttgacca gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg
    26341 gtaatcttga tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga
    26401 ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac
    26461 ttgtggcaat gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc
    26521 catggaatgc cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag
    26581 acaagttttg atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac
    26641 ttggaccatc ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc
    26701 tgttcctact gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa
    26761 catcccttta aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt
    26821 ggttttttct atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt
    26881 atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc
    26941 tgcagttgca cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat
    27001 atagaaaaaa gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt
    27061 aatcgatttt atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat
    27121 cgatgttgac tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc
    27181 cagcaaatgc gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt
    27241 gtcgcaggct cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg
    27301 ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga
    27361 caacatacca tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt
    27421 tatgaacgat ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa
    27481 cgacacggaa aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt
    27541 ttactttgtt ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt
    27601 ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt
    27661 ctttcgcttc attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag
    27721 ccaaataagc tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa
    27781 gtttcgatga ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt
    27841 ttgatttcat cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt
    27901 atttcgtttc tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca
    27961 aacgttcgcc gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg
    28021 aacaagcgag tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga
    28081 cttatacttc ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc
    28141 tattgttttg gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt
    28201 ggaggtatag cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt
    28261 acagagacta gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt
    28321 gtgccgatct tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc
    28381 tatcgtcata aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac
    28441 gctacaaagt tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag
    28501 cttccttata ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt
    28561 acgttgctta atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca
    28621 attgcttcca caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac
    28681 agcagcattg ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca
    28741 ttgatcacgc taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta
    28801 gagaaaaaga attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca
    28861 aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac
    28921 gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga
    28981 cgataaactc acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg
    29041 gaactaacat ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta
    29101 aacgcatctt tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca
    29161 atgtttggcc gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga
    29221 cgatgacccc gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca
    29281 aactttctga aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta
    29341 acgctagata aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc
    29401 ttttcgtttt ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg
    29461 atgaaccgcg gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc
    29521 cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg
    29581 tgggatatgg tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag
    29641 taggatctgc aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat
    29701 ttgagcaacc tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc
    29761 cgaaccttgg aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt
    29821 gtttttcggt cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt
    29881 aattttgtaa cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc
    29941 ttgctaatta ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag
    30001 caggaggaaa tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga
    30061 aatggctata aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat
    30121 acacttcatt atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa
    30181 gaaacattga ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg
    30241 atcatcgtag gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa
    30301 gtagaattcc cagttgctta tattccagca ggttctggag atgattttgc tcgaggggca
    30361 ggccttccaa aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa
    30421 aaagtccaca taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat
    30481 aatttcggta taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa
    30541 aagcgattga acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct
    30601 ttattcactc aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt
    30661 tcaaacgcat ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt
    30721 gttccaactg ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat
    30781 ttctttaaaa tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa
    30841 tatttccatc actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt
    30901 caagaagatg gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa
    30961 cagttgtttt ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta
    31021 ttcggaaaaa aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa
    31081 ttctttgccc aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc
    31141 attgaaaaca ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca
    31201 aatgtttgat tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat
    31261 gttggtgtga atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac
    31321 accccaccga taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct
    31381 gagtttttta ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag
    31441 aaagtattta aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga
    31501 accattagaa gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt
    31561 actttgcgat tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct
    31621 ttgcgattaa atgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca
    31681 taagacaaga aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc
    31741 gcacttagct caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca
    31801 tcgattgttt tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc
    31861 tgtgtaacaa ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcggcatc
    31921 tttcctactt cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga
    31981 ttaaaataaa gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt
    32041 tccttgtcat tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc
    32101 aaggcgattt tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag
    32161 aatgtggcaa caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc
    32221 aatgtccaaa tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc
    32281 taacgcaaaa aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa
    32341 atcagtgtag aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg
    32401 accaacaatc ggaatatgaa gatcaatcca atacttcact tgttccgacc ccatcatacc
    32461 agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt
    32521 catttttggt atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag
    32581 agcggtaaca atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg
    32641 aaagacagct aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt
    32701 agcgattgaa actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga
    32761 ttgatttgct tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc
    32821 cattccaatc agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac
    32881 ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc
    32941 cacttctgcc actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt
    33001 tttcatcaac gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc
    33061 aatgagataa aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga
    33121 tagttcctgt acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt
    33181 aaaattttcc gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc
    33241 gagattgcca ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat
    33301 tggaaaggcc aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata
    33361 tttataaaaa tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa
    33421 tctctaaaaa gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct
    33481 tttcttcttg taagattgta agaatcggca acaatactgt atttagttca gtagttgtta
    33541 cttggagctg acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg
    33601 ttttctggga gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt
    33661 agagattctt tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg
    33721 caaagaattt ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga
    33781 ttcagctcct tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc
    33841 ggctcatcta aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga
    33901 cgtttcaagc caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact
    33961 aattgaatca cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg
    34021 caaaaataat cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga
    34081 acgaccccaa tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat
    34141 ttaatggttc ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc
    34201 ccactgccgt ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa
    34261 tgattcaatg ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc
    34321 atgaaaaact ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa
    34381 ctagtgacga ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca
    34441 taaatctcgg ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt
    34501 aagtcaagga ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg
    34561 tgtcagttat ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt
    34621 atgtgccttt ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc
    34681 tgaagaaagc caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg
    34741 taatcatccc cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt
    34801 acgaaaaaaa gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac
    34861 tcattgggaa aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga
    34921 aacacaatta gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga
    34981 caatgtcggt catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa
    35041 tcaagaacca aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat
    35101 ggatcggatt cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc
    35161 cttagagttt ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga
    35221 aggattgaat gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa
    35281 cagtatcaaa catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt
    35341 ttatcgcttg caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat
    35401 tggtttgaaa acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca
    35461 agaaaaccag caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca
    35521 gtagtcatta ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca
    35581 cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg
    35641 aaacccatcg tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg
    35701 atgctgccaa agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat
    35761 tcaaagatga agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca
    35821 aacagaactt gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat
    35881 ttggtaatga aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata
    35941 gtcatgctta tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg
    36001 ggaaaaacaa taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt
    36061 acatcagcca attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt
    36121 tttatcagca tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg
    36181 catcattcag gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga
    36241 ttgaaagaat caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg
    36301 cggaaaaata ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg
    36361 ttctacatca ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa
    36421 gtcattgcca gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa
    36481 gtaacgcccc ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc
    36541 taaccaacga tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat
    36601 aaaatcgtag ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat
    36661 cacttctgct gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc
    36721 ttctccaaca aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt
    36781 ttcattcgtc ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt
    36841 tgtatagttc ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag
    36901 tttggtccgg agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt
    36961 catccggctt gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa
    37021 aatcaacagt tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg
    37081 ctgatattga tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg
    37141 gattgagcaa caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc
    37201 atttgcatca aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat
    37261 gatacccgtg atttctccca tatctccatt gaatacattc aattcgggag tattgactaa
    37321 ttggaggact ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc
    37381 aggtttccct ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg
    37441 tccccgatac attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc
    37501 tttttccaca attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga
    37561 acgatctttt tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc
    37621 aagaggaata atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg
    37681 aatttgttcg atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg
    37741 atctttgtct cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt
    37801 agccagccat gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc
    37861 tttcgtactg actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt
    37921 actggcaggc agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc
    37981 aagtagaata ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa
    38041 ctctgcaaaa agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc
    38101 tgttaaaata aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc
    38161 atactgtatg ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg
    38221 ataatggatt tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc
    38281 agaaaagtac aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc
    38341 aacaagttgg ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt
    38401 ttctaaaata cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt
    38461 ttgaactgca tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc
    38521 tgctgcaata ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat
    38581 gtcttcgact agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc
    38641 ctggtagata ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac
    38701 ttgatccatc ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt
    38761 caaaccggag atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc
    38821 tcccagcaat tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct
    38881 ggaaagatac gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag
    38941 tttaagctgc cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc
    39001 attttcttga atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc
    39061 tgtggtttca ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa
    39121 aatcgcggca actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct
    39181 ccttcttcta tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc
    39241 atactttcag ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac
    39301 tactattgaa caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt
    39361 gaatggctgc agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt
    39421 gggcaaccgc atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc
    39481 cgtgaaaagg agaaggatca tacagatcta aatgattacg caaattcggc agattatccg
    39541 gatatttctg gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc
    39601 ttagcttatc tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg
    39661 ctgtatcacg tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta
    39721 cctttttccc aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat
    39781 tgctcccttg aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata
    39841 gttccatacg cagcaaactc ctgtctttct tttattttac tctataataa actgtctaat
    39901 ccagcttgtc attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa
    39961 gtgcttcata cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat
    40021 cattcatagg gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac
    40081 cggttgtttc ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac
    40141 tatatacttt atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta
    40201 aaaaagcttc taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg
    40261 cccgttttag atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag
    40321 tattcgtggc tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa
    40381 ataaaacgat atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct
    40441 gacaatttga tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg
    40501 ttacgttagt gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa
    40561 tgaaaaaaca acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat
    40621 taaaaactgc tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta
    40681 atcttcttca aaggattctt atataagtct gctttgattc gctcttctac ctgccattct
    40741 aaaggtgaca tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt
    40801 acacgctgac gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg
    40861 gaataactct gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa
    40921 ttttcttgtg gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct
    40981 tggtaatgag aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc
    41041 aaattggtca aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag
    41101 actccttctt ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt
    41161 tcattcaaga agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa
    41221 tgctgcgcaa tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt
    41281 ggaacgaaca ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt
    41341 ccttttttgg aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt
    41401 tttacttctt cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg
    41461 cgtcctcggt caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta
    41521 gatgctcact ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata
    41581 aaggagaaga ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag
    41641 caattgctag aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa
    41701 ccccgagtac tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg
    41761 taatgacgat tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa
    41821 tcatgcatac tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt
    41881 gtcttactcc ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag
    41941 aagggttgca gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg
    42001 aagcaatcga ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg
    42061 ctgacaaaac tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg
    42121 gccgcttaat cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta
    42181 aagaattgaa cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt
    42241 ataaacaaaa aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta
    42301 cataaaatag cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc
    42361 gctcaaagtc gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat
    42421 tagcatcggg attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat
    42481 ccctatgaca aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt
    42541 gatccccgta acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac
    42601 aaatccatga tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg
    42661 ggttccagca ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc
    42721 ttcacttacc aagtcttcat agtgactgat taaagaagcg gtcgacatac ctaaaaacaa
    42781 cagagtgatc agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc
    42841 ttccacattg accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc
    42901 gatgatatca tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag
    42961 agctaagaag tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg
    43021 aactgaaagc actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat
    43081 taggtcgcgc agagaaataa cccctactag ctgaccttct tgattaacga catagatgta
    43141 atagattgtt tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt
    43201 ttgattggct acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc
    43261 gtaatggaga agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt
    43321 agcaatttgc tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc
    43381 agaaagcata tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc
    43441 gttgtcttct tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata
    43501 gatcagctgc ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg
    43561 catctctaaa aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa
    43621 ctgggcaaat ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat
    43681 tcttcaaacc gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc
    43741 aactcttctg taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt
    43801 attcccagat ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg
    43861 gaaaaatgaa ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg
    43921 atttctgtta gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag
    43981 accattcgct tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt
    44041 gccgataaat ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt
    44101 tcacgaagtt gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa
    44161 ccagatgtat cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt
    44221 tttttataat aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga
    44281 atagacgaaa cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata
    44341 tcgatcggtg tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg
    44401 gttaccttat catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg
    44461 atttgaccgc cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt
    44521 aaaaaatatt ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca
    44581 atcctcacta ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc
    44641 aaaatggatc cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac
    44701 attcaattga tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca
    44761 ttcatcatgg gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga
    44821 agcaatctct gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact
    44881 tttattatat gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact
    44941 ttcaaacagc tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat
    45001 cgttgattca ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg
    45061 aacatccagc aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag
    45121 ttcttccagc tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc
    45181 caagaaacgc acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc
    45241 gtctccgccg actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg
    45301 ttctaaaagt gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac
    45361 gatcgctact ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat
    45421 agtagacatc atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt
    45481 cttcacggat actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa
    45541 gtcgttcact catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa
    45601 tcgttgccca gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct
    45661 caccgctgat cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg
    45721 cttttttatt tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga
    45781 tgatctcaac aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa
    45841 tatcagacat ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta
    45901 caatactatc gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt
    45961 gttcacggaa ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt
    46021 catatggtgc taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa
    46081 cattcattgt ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg
    46141 accaaaaaaa gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc
    46201 ttgaaatcga atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact
    46261 atcaattaac agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg
    46321 aacagctgaa gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat
    46381 atacattaaa aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag
    46441 aagaagcaga agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa
    46501 aactttctga tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa
    46561 aacgggctga attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca
    46621 atctacacga ttttgaactg gaactagagg taggggatgc atctgtggga aaaattgctt
    46681 ttataaattt tttaaaacgt ttttctttac cttatcgtcc tgctaagaac aagatccaac
    46741 gtatgcttga agctaaaaat tagaaacctt tcattttcac aaggcacttg agttcatttt
    46801 tttacttttt tctgttaagt tataggatgt gaaagaatct agtcactagt tgtatagaat
    46861 ctattttggg ggagattcat atgatcgaaa tctatttatt tgttaatcct ttaggggaat
    46921 actgtttcga aatggaacaa cagttattac aatttatcga agaagagtac gggacaccat
    46981 caaaagaaaa gatgcaattt cgctttcttc ctttggtcaa tttgcagaca attggggatg
    47041 tcatgcaaca aaaaggaatt tctcagaaca atcttgagga acgtaatcac ttattctcaa
    47101 tgacttattc tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc
    47161 gccgtttttt gatcaaattg caagaagcag tcggctgtaa tgacatacca tattctcaag
    47221 aacttgctga atccatattt gaagaaatcg gcggcgattt gaatatgttt aaagaagatc
    47281 gtcagtctga cttcgttaaa gaagtattct tttctgatca aaaaattgct cgggaaatgg
    47341 gcatcacaaa acacccttct gcagtcgttt acaattatgc ctgcgaccgt gattttggtg
    47401 ttttagtaga agtaaaaggt acaagtagcc tttggagaat cacagagctg tgtaaaacaa
    47461 cagataactc atatcaagtt tttaatggtg aacatttaca tacaaatgat tctcagttcc
    47521 attcaagaac tagccattta cacttgcttt ctaattaaaa aaatacgtcg actcccttac
    47581 aagagagccg acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag aaagccttat
    47641 aaacgatcga tcaattcttc taattcattt agacgttgtt caaacatcga cattgcatct
    47701 tcaatatatg cagcctgtgt catatcaact cctgcttttt tcattacttc cacaggataa
    47761 tcgctgttac ctgcttttag gtaagccaag tagttctcta gtgcctctgg ttcctggttt
    47821 agtattttct tcgccagtgc tgaagcagca gaaaagccag tcgagtattg gaaaacatag
    47881 taattataat aaaaatgcgg tatccgtgac cattcgaatt taatttctgg gtcttcttcg
    47941 actgctggac cataatattt tgcattcaat ttaccataac tatcacttag gtattcacta
    48001 gtcaatggca cacctttttc atcttccgta tgcatgaaat gctcgaattc agcgaattgt
    48061 gtctggcgga aaactgttcc tttaaaccca tccaagtaat ggttgagtac ataagcccgt
    48121 acacgagggt ctttttctgt ttccaataaa tactccgtta ggatattttc attcgttgtc
    48181 gaagcaattt ctgctaaaaa gatggagtag tcgccgtaca cataaggctg gttcgaacga
    48241 gtgaaataac tatgaacact atgtcccatt tcgtggacaa gcgtaaatag ctgatccagc
    48301 gtatcatgcc aattcaataa aatatatgga tttgtgtcat agcttcccga agaataagca
    48361 ccgcttcgtt tccctttatt ttcgacaaca tcgatccaac gttcagagaa tgctttttct
    48421 acgatggcca tgtattcttc acccattggt ttcagtgctt ctaaagcttt ttcttttgct
    48481 tcttcgtacg taaaggtaat tggagcttcc cctaagaccg gtgtataaag atcatacatg
    48541 tgcagttttt ccacttctaa taaacgtttc cgtaattcca tgtatcgatg taacaaaggc
    48601 aaatgcttgt ttaccacgtc taccaaagta tcgtatacac tttcaggaat atgattattg
    48661 ctcaaagacg cttctctggc agagctgtaa ttacggactt tcgctttaaa attatgtcct
    48721 tttatatgtg tgcctaaagt agaagcaaat gtatttctaa attgttcgta aacactgtac
    48781 aatcccttaa atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa ctgaccatac
    48841 acgccatgag ataattggac tatttcacca ttttctcctt caatcgttgg aaaaactaga
    48901 tctgcattat tcaaaacagc aaatgtatca cttgaagcat caaagatttc acctgctcca
    48961 gcaagaagag attcctgctc agcagataag acatgggctc gattatctac tatttgctgg
    49021 atataatggc gatagacttc caattttggt tcttctttga aatattgcca aatctggtca
    49081 tctgacaatt gcaatatttc tggttcaaac caggaaacgg cttcactaac tttcgaaaac
    49141 agactgcttg ctcttgcata aagcgcttgg taatctgtat ttccagtgtc ttgatcgttt
    49201 ttaaggtgcg catatacata aatgacttca gtttggcgat aaaccctcaa tacgaattca
    49261 atcgcattta aaaattgaga agcgccttga tcaagtgttc ctttgtgttt ttcagattgt
    49321 tttaactctt ctgataattc caaatatttc tcatcaaact cttggtcgct tgaaaagatc
    49381 ttggtcaagt cccaagttaa attttcaggc aattcttctc gttttggcaa ctgctttact
    49441 tccatgaaat ccactccttt tcttccctta gctctatcta tcaaaataga tcatagtcta
    49501 acatccacat gtcacagcat ctaacttttc tactcattca tgtgtctgct tccatcaatt
    49561 ttagcataac taaatcttgt tttacataga aaaccgattc ctagcctatt tttatactta
    49621 ctattttttc aaagaagaca agtgtatgca ttcgttaaaa aactgctgat agaccatctc
    49681 ttcatcaatc ataggaaaaa gccattccct tttatgttct tttatttggc aaagccatga
    49741 gttgaaatca ggaactttct ggttttccaa ggcttcttcg tacaacattc gataaagaaa
    49801 aacctgttct tgtaaataaa agaaaaaccg acttttttga tagatccacg atgatagatg
    49861 caaaagatgt ttatgacgca agtagcattg ttcttgtatg gatactgtat tcttttgctt
    49921 gtgaataagt tggcgtgtca gccatttctt cccatcttca ctcacttttt ctattttttt
    49981 catttggttc attttaattt cgttactagt gaatatttct ttaaggtccc tactagaaaa
    50041 agtccagcag ctactgcaaa aggaaatatg ccctgataca gtctctgtaa tatgatgata
    50101 caaataaagc ttttgtttct tgagatcagc tttccagcaa tgcactcctc tttttctatt
    50161 atagtagcag caactttttt ctatcaatga gaattgcgat tgtccaagaa aagaattacc
    50221 aagtatccac catacttggt aactgttatt caaataattt tgggttcttt caataaatct
    50281 ctggtgagac agtctagaac attggatttc tattgctaga ttcgccaaca aaatatccgg
    50341 cctttgccgc aacctcggca aataggcttc caaaaatact ggttctactg actgtcccag
    50401 ccaatccatg aagcattcct ttaactgtaa gtgctctttt gattctcttt cactaaagac
    50461 cgaacagtta tctgattttt gatgggcaaa atgagcacat tttttctttc cctttttcaa
    50521 aatcactctt tttcgacagc cacgacaata atatttcttt cctttttctg catgtttagc
    50581 aaaacagcac ttattatatt cattcattgc aagcagcatt tttctccctc tgaacttatt
    50641 tacgtctaag aatttattta cacgaaaaaa gagggcgtga aaaagctatt ctgcatcaag
    50701 taataagaac agctaaacaa aaatagttcc caatattttt cgtttggttg ggcttgttac
    50761 cgcagatgca agcttttgaa cacatttata cgaaaaaaag actgcgacac aatttttgtc
    50821 acagtctctt actataatgc tgatttatac aaaataaaat ttcgttcgct cgatagcatt
    50881 acgcgccatc agacattgtc catattcatt caatgtttct ggtgtacgat cgctcttttc
    50941 agcaaactcc aaaattcgag caatctgatt ctctacatcc gcttggttga gattttccat
    51001 ccagaagtga acgactaggt aatagcgctc ttcgaataaa tacaaatcag tccaagcaga
    51061 ctctagttcg acatctgttg caagttggat cacttgttca aaatcttcaa aagcaaaagt
    51121 tgcttgtctt tcctgttctt ctagggcgct tccagataat tcttcctccg gatcgctgcc
    51181 aataatctgc tgacgaagaa agtcactcac atcttctgaa tcaacagtag aatcttctga
    51241 ttgagaaatt tcttcatccg gaagattttt actgataaac agttctaatc cgtctccttt
    51301 tggcaaaact tggaaagtga cagcttcgct tcctcggaac tcctcttcga tatcaacttc
    51361 ttccaaaata ctataaaaga aattttcgat ttctttgtga ttgccaagta aatccaaaaa
    51421 tgaaacgccc cgttcctcta aatcttcatg accaattagt acacggatcg tattttcatt
    51481 aatatgttcc atttccattt tgctacacct cactttgcca caatgtttaa tatagattca
    51541 ttgtagcgaa atcagcttaa atgtaaagaa aaaaaatctc gctgttaagc gaatttaata
    51601 aaaccagcgg atttttcaac ctttcaagct atcttattta actataacat ttttggcaga
    51661 cgaatactat atagccgact tttcagctaa ttttccctct ctatttttcg tgcaatcatc
    51721 atcttctact acctttttat acatctaatc aagaatactt tttgtaaaaa caaaaaaact
    51781 tggaaaaact atctttgtaa tagt
//
LOCUS       DACCCR010000011        48702 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCCR010000011 DACCCR010000000
VERSION     DACCCR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.011"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            23..646
                     /locus_tag="IVK93_000655"
     CDS             23..646
                     /locus_tag="IVK93_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385970.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IVK93_000656"
     CDS             1096..2145
                     /locus_tag="IVK93_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6385971.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IVK93_000657"
     CDS             2142..3023
                     /locus_tag="IVK93_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6385972.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IVK93_000658"
     CDS             3026..3796
                     /locus_tag="IVK93_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6385973.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IVK93_000659"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IVK93_000659"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP6385974.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IVK93_000660"
     CDS             5107..6270
                     /locus_tag="IVK93_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6385975.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IVK93_000661"
     CDS             6387..6797
                     /locus_tag="IVK93_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6385976.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IVK93_000662"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IVK93_000662"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6385977.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IVK93_000663"
     CDS             9337..9654
                     /locus_tag="IVK93_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6385978.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IVK93_000664"
     CDS             9814..10050
                     /locus_tag="IVK93_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6385979.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IVK93_000665"
     CDS             10047..10445
                     /locus_tag="IVK93_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP6385980.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="IVK93_000666"
     CDS             complement(11112..12056)
                     /locus_tag="IVK93_000666"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6385981.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="IVK93_000667"
     CDS             complement(12352..13296)
                     /locus_tag="IVK93_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6385982.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="IVK93_000668"
     CDS             13517..14731
                     /locus_tag="IVK93_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6385983.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="IVK93_000669"
     CDS             14753..16768
                     /locus_tag="IVK93_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAP6385984.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="IVK93_000670"
     CDS             complement(16851..18224)
                     /locus_tag="IVK93_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6385985.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="IVK93_000671"
     CDS             complement(18250..18534)
                     /locus_tag="IVK93_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6385986.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="IVK93_000672"
     CDS             complement(18536..20551)
                     /locus_tag="IVK93_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6385987.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="IVK93_000673"
     CDS             complement(20730..21035)
                     /locus_tag="IVK93_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP6385988.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="IVK93_000674"
     CDS             complement(21124..22155)
                     /locus_tag="IVK93_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP6385989.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="IVK93_000675"
     CDS             22362..23237
                     /locus_tag="IVK93_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6385990.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="IVK93_000676"
     CDS             complement(23389..24231)
                     /locus_tag="IVK93_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6385991.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="IVK93_000677"
     CDS             complement(24323..26200)
                     /locus_tag="IVK93_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6385992.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="IVK93_000678"
     CDS             complement(26197..27618)
                     /locus_tag="IVK93_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6385993.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQAATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQTEIAKNGA
                     SSGTFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKAPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGISTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="IVK93_000679"
     CDS             complement(27621..31010)
                     /locus_tag="IVK93_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP6385994.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDCVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNSWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKNLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="IVK93_000680"
     CDS             31267..32658
                     /locus_tag="IVK93_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6385995.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="IVK93_000681"
     CDS             complement(32731..33378)
                     /locus_tag="IVK93_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6385996.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="IVK93_000682"
     CDS             complement(33386..34072)
                     /locus_tag="IVK93_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6385997.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="IVK93_000683"
     CDS             complement(34078..35151)
                     /locus_tag="IVK93_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6385998.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="IVK93_000684"
     CDS             35373..36047
                     /locus_tag="IVK93_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6385999.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQPPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="IVK93_000685"
     CDS             36052..37392
                     /locus_tag="IVK93_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6386000.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="IVK93_000686"
     CDS             complement(37541..38527)
                     /locus_tag="IVK93_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP6386001.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDENLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39251..40282
                     /locus_tag="IVK93_000687"
     CDS             39251..40282
                     /locus_tag="IVK93_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6386002.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40391..41923
                     /locus_tag="IVK93_000688"
     CDS             40391..41923
                     /locus_tag="IVK93_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP6386003.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIISLSPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42062..42874
                     /locus_tag="IVK93_000689"
     CDS             42062..42874
                     /locus_tag="IVK93_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP6386004.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42864..43349
                     /locus_tag="IVK93_000690"
     CDS             42864..43349
                     /locus_tag="IVK93_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6386005.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43372..43917
                     /locus_tag="IVK93_000691"
     CDS             43372..43917
                     /locus_tag="IVK93_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP6386006.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(43990..45633)
                     /locus_tag="IVK93_000692"
     CDS             complement(43990..45633)
                     /locus_tag="IVK93_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6386007.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45762..47990)
                     /locus_tag="IVK93_000693"
     CDS             complement(45762..47990)
                     /locus_tag="IVK93_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6386008.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(48023..48502)
                     /locus_tag="IVK93_000694"
     CDS             complement(48023..48502)
                     /locus_tag="IVK93_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386009.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        14818 a         8979 c         9169 g        15736 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgattta tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcacatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaaac cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca ttcgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttgat ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac aaatttcagc gtaccaccag gagtgagtga aggaatataa ttcgagtcaa
    24961 ctgaaaccgt gaatccgttt gtttgttcag tgacagaata atttgttgga tcgatttctt
    25021 tatccccatc atacaagaca tacgcatacg ttcctgaagt ttcatgatca aatgtcaaag
    25081 cggcgtcatg agtatcaatg agtttgaatg tcgtgtactt gttttgtgtt ccttctttat
    25141 cagcgatacc taaaggaata ttgacagaga tctcatattg gattttttct ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt ggacgtatca tttttgattg
    25261 tcttttctac aggagtttga ctatttgcca agatctcaaa cggcgtgttt gtttgattat
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacctcta
    25381 aatgattgac gatcaactga cctttttcaa tcgttgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatccgtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtcttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgatac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt ttttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tgctttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cgtaccggaa gacgcaccgt tttttgcgat ctctgtttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataacgcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct gcctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcggta tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaagacag caccgatgag attcttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacga atttgtagta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac aatccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga atgaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgtcacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagcc tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc accatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagcactt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 agttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata aaatgtcatt
    38701 tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg tgatggttta
    38761 atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat tctcaatata
    38821 attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac tatttctaat
    38881 acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa taaatggaga
    38941 gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa gagcctcctg
    39001 ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag ctaaaaagag
    39061 gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta aaaatgtatc
    39121 ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta agatcaagac
    39181 aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac actattggag
    39241 ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa agcaacgtac
    39301 acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt ccctttcatt
    39361 aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt tgagaaaagt
    39421 attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag attatcagaa
    39481 tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga accaagaaac
    39541 ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt cattaaatca
    39601 ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc ttttgggtac
    39661 aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac ttttcaaaga
    39721 ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag ctagctttga
    39781 agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt gcggattaaa
    39841 gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc agattcttaa
    39901 tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag cgatgccatt
    39961 tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt ttgtttttcg
    40021 ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga gcaaaattac
    40081 agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt atgcagaact
    40141 atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc tctcatccca
    40201 ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat tagcggcatt
    40261 cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat aaactatgtt
    40321 attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa caaaagataa
    40381 tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta cgaattttca
    40441 agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac tttcagcttt
    40501 cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc
    40561 ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt ataagagttc
    40621 cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat
    40681 gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt tagaagaccc
    40741 tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa
    40801 atcaaaacag attcctattc tttatttaat ggaacaacct gctttgtctg tttctcaatc
    40861 atttttagac aaaaatgaaa tttcttatat gacccatact tctggtacaa ctggtatccc
    40921 taaacttatt tgtcattctg ctcattctat gggatggcgg acgaaatggc aaaaaacagt
    40981 tttcacaaaa atttcggaaa agaaattaat cgcttttcac atctctccag tgcattccag
    41041 attcaatatt ggtatctctt ctttgatgtc catgggattt cctatgatgc ctttagcaaa
    41101 cgcacaaagt tcaaaagttg ttcatatgtt ggaagctcac cggccaattg cactagagac
    41161 ccatccgaat aactttgttc agtggcgttt tactgctaag gagcatccag aagctttttc
    41221 gggtatccga tactatcact ccacttttga tgctataaac aaccagacaa tgaagacatt
    41281 tttgcgaacg tcgatggcac aggatgcggt atttcttcaa gtatatggcc aaagtgaatg
    41341 cggaccaatg attcttaagg ctcatacact agagagtttg aaaacaagtg atgcaagaga
    41401 tatgggcgtt ggactggaag acatgacaag cgctcggatt acagattcag ttgggaatgt
    41461 cctacctgaa aacacagatg gtcatataca attactgtct aaaggacggg ctttgactta
    41521 ttacaaggaa gatgcacggt tccaggaaaa tgtttatggc gattggtggg atagcggaga
    41581 ttacggtttt atggataagc atggccacct atttttgaaa gatcggcaag tggatttgat
    41641 cgaaacgatc aatagtacat tagctattga agacttttta ttagattcgt tagatttttt
    41701 ggcagaagta gtgattgtaa gggataaaaa tcattctcca caaccgatta tttctttatc
    41761 tcctgataaa gaaatggatt ggaatcgatg gtgggagcaa gtccatgaac ttcctcgctt
    41821 gaacgtccct atcatccgtg attttgatgc aatcccgcga acagctacaa tgaaagtgca
    41881 acggcttcaa atcgagaaag aattaaaatc acaaaagtcc taactattta aggaaagaaa
    41941 gatccatttt ggtctttctt ttctttttgt ttttttaaat tggatgagat aaaaatatgc
    42001 caattggctg tttttctttt ttgaaaattt cgttatactc ttattcatgg aggaaaatgt
    42061 tatgaaaaga atcttgacaa ttggcggttc tgatccattt gctggtggag gaatccaaac
    42121 agatttaaaa acttttgaga attttggtct attcggacta agcgccttga cttctatagg
    42181 ggcactagat attaatgagc gatttatgct ggaatctata tctttggaat tgcttaaacg
    42241 acagttagtt tcgattgata aaatgacttc attagatggt atcaaaattg ggttattgaa
    42301 ttcaagtgaa gcaatcatgg ttgttcgaga cttcataaaa acaaaacaaa atatcccaat
    42361 cattttagac cctgttttgg cattcaaaga aactgaagct gtgatcaatc aattgtatat
    42421 ttcgcagctc atcgaagaac tttttccact tgttacgatg attacgccta atttggcaga
    42481 agctgcttta ctttctggtc aagctcttcc ctcttcgtta acagaaatga tccatctcgc
    42541 taaagaactc actgcttcag gaacaaaaaa cattgtgatc aaaggcggca caaggatggc
    42601 tggagatgaa gctgtggatc ttctctattc aattgatcat tatgaggtat tttctcataa
    42661 aaaaatcgac actacaacag taaacggggc cggttgtgcc ttttcttctg caataacagc
    42721 gaatcttgtg ttaggggcag attttaccaa agcagtagca gaaagtaaaa aatttgttgc
    42781 ccattgtatt tctaatggcg tgctgatgaa cgatcatacc ggaagtgtct ggtctggcgg
    42841 tattcaaaaa ggaggaatga gaaatgaaaa ctaagcaatt aacgatctat gctatgctga
    42901 ttgcgttgac agttgccctt tcattaacaa ttttgatccc tgtaccggct acgaatggat
    42961 ttgtgacatt atgcgaggcc gggatctata ccgctgcttt tctttatggt ccaacgggtg
    43021 gattagttgt tggtgcttcc agtggtctat tgatcgattt gatttcagga tatccgcagt
    43081 gggcagtttt ttcattagtg atccacgggt tacaaggata tgttgctggt tctttcagca
    43141 aatcttctac tcgtatgtgg ggattagggc ttttattagg aagcttagtt atgatcatcg
    43201 gctatggtat cgccggttgg attttatatg attggccggc tggagtggct tctataccag
    43261 gaaacatctt ccaaaatctg attggaatgg gacttgcctt tcctataact gcaagtcttc
    43321 aacgtattca agcacggaag caatattaat tgaaaaagaa ggttttagaa aatgaagttg
    43381 tcagaaaaag aattaaaaga ccaattgact gaaatagtta atgacatatt ggctgaggca
    43441 cacttgaaaa aaggtgatct ttttgtttta ggatgcagca caagcgaagt cgttggagga
    43501 catattggaa aaaattccag tgcggaagtt ggtcaatgga tcatccgtac gttaaaagag
    43561 ttgttggatc ctaaagaaat tgcgctagct gtccaaggtt gtgaacattt gaaccgagca
    43621 ttagtagtag aacgaacagt ggcagaagca aaaaatttcg aaattgtctc tgttgttcct
    43681 gcactacatg ctggaggtgc ttgttctgtt gctgcttttg accagttcaa tgatcctgtc
    43741 gaagtggaac acgtagttgg acaagccggt atcgatatcg gtgacacatc aatcggtatg
    43801 catatcaagc atgtccaagt acctgtcaga cctcgtttga aaacccttgg acaagctcac
    43861 gtgacagccc ttcgctctcg tcctaaatat atcggtggtc ctagagcgaa ttattgaaat
    43921 gagtaattga cagagcaatc tgaaaaactt cctcgcaaag tccaactaat gaactttaac
    43981 gaggaagttt tacttgtttg ttttatataa ccgagcttca taaggtgcta gtatgccttt
    44041 agcttcaaat tctccgctag aggaatttga caagagtaat gtccaggact gatctgcttc
    44101 ttttggaagt tcatagtcag caagtgattc tgttaagttt accataataa gatattgttc
    44161 atcttctaaa tatctttcat agacataaag ttgtggatgt tcatgaagat attctttata
    44221 agaaccgtat atcagacctt tattggtttg tctaatgcgg atcatgttct tatagaattg
    44281 caggaccgac tctggttcat attcttgttg ttctacattg atcgttttgg tatttggatt
    44341 agtgatcagc caaggttcat gtgtagaaaa accagcatat tgttccgaag tccattgcat
    44401 gggtgtcctg ctgttgtctc tcgttgtttc acgaatgata tccaatgctt cttctcttgt
    44461 cttaccttcc gcaagcagcc ttttataaag tcttttcgaa tctactgcat caacttgatc
    44521 gatcgattca aagggcatat tcgtcattcc gatttcctgt ccttgataaa taaatggcgt
    44581 accttgcaaa aagaaataca tcatagcgat tgcttttgca gacatagccc aaaaatcggg
    44641 acgagtatct ccaaacactg aaacggcacg aggaacatca tggttttcca tatataaagc
    44701 gttccatccg ataccatcca atgaagtttg ccaagcgctt aatgcatgtt tcaatttaag
    44761 gacatctaat tgcccttctt tttcttgctg ccagatatgg atatgatcaa attcgaatat
    44821 catatcgaaa taaccgtctt tccctaccca ttgtggtcct tcttctgcag tgacaccact
    44881 tgcttctccg acagtcatga tatcgtactc ttcaaaaaca tgtttcaaat cggtcaaatg
    44941 ttcttcgatt cctgagacat tttgaaaggg tgagaaaggg ttctctgtgg cttttactga
    45001 atattcatct ttttttacat gagaaatcgc atctaaacga aatccatcta tccctttatc
    45061 caaccaccat cggatcatat tgaatagttc ttctttcaat ttctcgcttt cccaatttaa
    45121 gtctggttgt tcttttgcaa aaacatgcag ataatactgt ccagtttcat gagaatactc
    45181 ccaagcagaa ccgccgaaaa tcgatgtcca attattcggt tctttgccat ctaccccgtc
    45241 gacccaaatg taataatcgc gatacgggct ttctttagat tttttcgatt cttcaaacca
    45301 ttgatgctga tccgaactat ggttgatgac aagatccata atcactttga tccctaattc
    45361 atgtgcttct tttagcaata aatcaaaatc ttccatcgtt ccgaatctct cttctatatc
    45421 ttgatagtca cttacgtcat aaccattatc gacaaatgga gattgataga tcggattgat
    45481 ccagataaag ccgataccaa gatctttcaa ataattaagt ttttcacgga tcccattgat
    45541 gtctccttga ccatcgccat tgctatcttt gaaactagca ggatagattt gatagcctac
    45601 tgcttctttc caccattttt gttcttgtat catatcgttt cactccttta acggtccctc
    45661 ttgattataa ccgattctat tttagaagta tccttgttac cggtaactag aaacgaaaag
    45721 ctcaacgaat ctgctaagtg cgttcgctga gctttatttt atcatagata gcgggcaatc
    45781 agttgaatca tttgcttggg aaattcattc aagtcagtaa tatctacaaa acgactttca
    45841 ccgtagatcg acttgatttc ttctttgtct tggccaatag ctgcggagac aaataggatt
    45901 ccttgacgtt catattcttt caaaacagac tggatatctt cttttgcttt tttgcctgta
    45961 taatccggca acgcttttgg ctgaccgtca ctaatattaa gcaacagttt ggttgttgcg
    46021 gattgtctgt ttaatttttc tgctgctaaa cgaagtgcag caccatcacg attgttttgc
    46081 cttggtttca tagtgactaa tttttcattt aaccattgaa atccgtcttc aaattcttta
    46141 taagaaaaca aagaagtctt ttctcttgac gaacggtctg ctgtatctcc gtaaatcaac
    46201 aatgggatat cgactttctt agaaaattcg gcaatggcaa tcgctgcttt ttttgccgct
    46261 tcgatccgat catcccgtat cattgaccca gattcatcga tccgtacagc taccgctaaa
    46321 gaaggttgct catgtggcgg gttctttttg tcaaaatttc tcaaatcacc atatgctact
    46381 ttgcttgcat tgaaccgctg accttcgtat tttccttttt ggtacgtctc tgtttgttca
    46441 ttatccagta actctaaaat ttgccggcta agtgattcta ctattggcat tacttgcatg
    46501 tgtagctcac gtgcttccgg cgtttttcct tggaaagctg gtcggtgaac gatgagacct
    46561 tctttttcat gaatcgtttc cttcatgatt tgtcgcgctt ctttattcag ttgcttcttg
    46621 atttccttat catgtgcttc catgtcagaa gcagacattt cttgagaatc atctcctgtt
    46681 tgttgttctt tttgattttt tccttcgata tcgctactgc gttctttttg catttgagga
    46741 ggaatctcgt ttttttctat tgtttcttct tgtttctctt ctccctcatt tggttcttca
    46801 gcagacaacg atttcttttc atctaagcgc agaatttctt cagcattttc agtgatgttt
    46861 cgttcatttg cttcttcggc gctaatggtt ctttcttttg tatgctcaga ttgaggatga
    46921 ccccattttt cttttctcaa tatgtcctga agttccagta gcaatccact gtcagacaaa
    46981 gcttgttcaa gaattgccaa ttcatctgaa tcagtggata atttatacaa aacttttgga
    47041 taaatcgact ggagtaaatc ttgtccttgc ttataggtat ttgcccaata aaaataagaa
    47101 cgcattcctg ctacaccttt gatggcgtta gcttttgcag tttcatccaa caaacggata
    47161 atctctgcca tctttaagag aagttcttgt ttttggatca tagttttcgc catcgcacgt
    47221 tctgccatga cttctaaggc aggaagatcc attttttccg catgttgcat ccgatctctt
    47281 aaagattcat ttaatgggcg atttccttgg taatttcgat ttgtagtgat cacaatgacg
    47341 caatctggat ggcgacgaat gattcctgtc ggtaaattca acatcccatt tggttcgagc
    47401 gctgagttaa gtgccacaag aacagaggca tctcggatga cagtcggctc ttgaatctct
    47461 aataaatagc ctttttcaat cgctcgaacg atttcagaag ggtaatagct gtaatggatt
    47521 gcatctggat aatcagcttc gatcctttga actagctctg ctaatttttc tttagcagaa
    47581 aaaggatcaa tgccgtaatg gttctggatc agtgtcagta cagcttcaag tgtatctgtt
    47641 tggtaaatag cttctagtaa ttcttggtct tgttccttat tagaatctac tacaggtaat
    47701 aaggctccga aaacatctga tttgtccata tctgcaaaac aggtcacttt tgtataggga
    47761 agttgcagat cagcagatag tgctttggct aattgtgtct tccccgaccc ggcatctcct
    47821 tccaaaagta cgttactgat tttcatttca ggatcttgcc aattcccttt gatttcggag
    47881 gcgatccgcc attcttctgc acttgtccga tggcttttcg gtttcttcca gatcatttgc
    47941 ttttctagcc gtgaaaaggt tcggttttct gttagtgaga aattctccat gcttctcttc
    48001 cccttttgat catctctgtc tattaatatc ctagctcatc taaaagacgt tttaaaatac
    48061 ggaatcgttc cccaatcagt tctccgtctt tttgtagtgt atcgtaatac aaaatgatgt
    48121 cttcatcgat ctttggattt ttggtatcta cttctaccgt cattcggttc ccttgtaatc
    48181 caatgatgtc tacgactgga ttttctggat cataaaattt ttcataacga taagcatctt
    48241 ggaagtataa acttccttgc gcagctaaga tcgctaaatc taaaatacgt gtgattggta
    48301 attcttccga ctgtcgtgac catttttctc cagtatgtcg ccataccttg cctgagatat
    48361 cgacttttcc acgatcattc cactgagcaa ggcccagtga aagccctttt gcatccatat
    48421 ttaatgcatt tcttccatca actttatcat agtcttctac aacgattact ggtttatgtt
    48481 ttaaatttgt tggtatttcc attattttaa ctcctcatcc ttttattttt agtaaataag
    48541 taaattagta gtttactcat ttactaaact atatgcgaca ttatagagga ttgaagtgag
    48601 gattgcaaag aaaaagagct tcattgatag aaataaaaac accatctcgt tcttttacac
    48661 gctcattagt aacatgtaga aagtccaaga tgatgttgga at
//
LOCUS       DACCCR010000012        47973 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCCR010000012 DACCCR010000000
VERSION     DACCCR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.012"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..333)
                     /locus_tag="IVK93_000695"
     CDS             complement(<1..333)
                     /locus_tag="IVK93_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386010.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
     gene            complement(508..2643)
                     /locus_tag="IVK93_000696"
     CDS             complement(508..2643)
                     /locus_tag="IVK93_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6386011.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2726..4381)
                     /locus_tag="IVK93_000697"
     CDS             complement(2726..4381)
                     /locus_tag="IVK93_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6386012.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4383..4697)
                     /locus_tag="IVK93_000698"
     CDS             complement(4383..4697)
                     /locus_tag="IVK93_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6386013.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4867..5127
                     /locus_tag="IVK93_000699"
     CDS             4867..5127
                     /locus_tag="IVK93_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP6386014.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5141..5455
                     /locus_tag="IVK93_000700"
     CDS             5141..5455
                     /locus_tag="IVK93_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP6386015.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5940..6494)
                     /locus_tag="IVK93_000701"
     CDS             complement(5940..6494)
                     /locus_tag="IVK93_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP6386016.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6620..8548)
                     /locus_tag="IVK93_000702"
     CDS             complement(6620..8548)
                     /locus_tag="IVK93_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP6386017.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGSILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8854..9570)
                     /locus_tag="IVK93_000703"
     CDS             complement(8854..9570)
                     /locus_tag="IVK93_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6386018.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9571..11394)
                     /gene="glpO"
                     /locus_tag="IVK93_000704"
     CDS             complement(9571..11394)
                     /gene="glpO"
                     /locus_tag="IVK93_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP6386019.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11408..12904)
                     /gene="glpK"
                     /locus_tag="IVK93_000705"
     CDS             complement(11408..12904)
                     /gene="glpK"
                     /locus_tag="IVK93_000705"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP6386020.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13040..14500)
                     /locus_tag="IVK93_000706"
     CDS             complement(13040..14500)
                     /locus_tag="IVK93_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6386021.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14490..15785)
                     /locus_tag="IVK93_000707"
     CDS             complement(14490..15785)
                     /locus_tag="IVK93_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6386022.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16117..16794)
                     /locus_tag="IVK93_000708"
     CDS             complement(16117..16794)
                     /locus_tag="IVK93_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP6386023.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16824..17915)
                     /locus_tag="IVK93_000709"
     CDS             complement(16824..17915)
                     /locus_tag="IVK93_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP6386024.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18016..18450)
                     /locus_tag="IVK93_000710"
     CDS             complement(18016..18450)
                     /locus_tag="IVK93_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6386025.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19034..20491)
                     /locus_tag="IVK93_000711"
     CDS             complement(19034..20491)
                     /locus_tag="IVK93_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6386026.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20488..22383)
                     /locus_tag="IVK93_000712"
     CDS             complement(20488..22383)
                     /locus_tag="IVK93_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6386027.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22441..24216)
                     /locus_tag="IVK93_000713"
     CDS             complement(22441..24216)
                     /locus_tag="IVK93_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6386028.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24266..25762)
                     /locus_tag="IVK93_000714"
     CDS             complement(24266..25762)
                     /locus_tag="IVK93_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6386029.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25956..27578)
                     /locus_tag="IVK93_000715"
     CDS             complement(25956..27578)
                     /locus_tag="IVK93_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6386030.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28028..29341)
                     /locus_tag="IVK93_000716"
     CDS             complement(28028..29341)
                     /locus_tag="IVK93_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6386031.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(29586..29810)
                     /locus_tag="IVK93_000717"
     CDS             complement(29586..29810)
                     /locus_tag="IVK93_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386032.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29830..30198)
                     /locus_tag="IVK93_000718"
     CDS             complement(29830..30198)
                     /locus_tag="IVK93_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6386033.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(30394..31104)
                     /locus_tag="IVK93_000719"
     CDS             complement(30394..31104)
                     /locus_tag="IVK93_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386034.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31122..31618)
                     /locus_tag="IVK93_000720"
                     /pseudo
     CDS             complement(31122..31618)
                     /locus_tag="IVK93_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(31655..32107)
                     /locus_tag="IVK93_000721"
     CDS             complement(31655..32107)
                     /locus_tag="IVK93_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6386035.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32242..32847)
                     /locus_tag="IVK93_000722"
     CDS             complement(32242..32847)
                     /locus_tag="IVK93_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6386036.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33297..33917)
                     /locus_tag="IVK93_000723"
     CDS             complement(33297..33917)
                     /locus_tag="IVK93_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6386037.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33919..34788)
                     /locus_tag="IVK93_000724"
     CDS             complement(33919..34788)
                     /locus_tag="IVK93_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP6386038.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34811..35218)
                     /locus_tag="IVK93_000725"
     CDS             complement(34811..35218)
                     /locus_tag="IVK93_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6386039.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35237..36526)
                     /locus_tag="IVK93_000726"
     CDS             complement(35237..36526)
                     /locus_tag="IVK93_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6386040.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(36553..36855)
                     /locus_tag="IVK93_000727"
     CDS             complement(36553..36855)
                     /locus_tag="IVK93_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6386041.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(36880..38833)
                     /locus_tag="IVK93_000728"
                     /pseudo
     CDS             complement(36880..38833)
                     /locus_tag="IVK93_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
     gene            complement(39322..40050)
                     /locus_tag="IVK93_000729"
     CDS             complement(39322..40050)
                     /locus_tag="IVK93_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP6386042.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(40196..42817)
                     /locus_tag="IVK93_000730"
                     /pseudo
     CDS             complement(40196..42817)
                     /locus_tag="IVK93_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(42931..44262)
                     /locus_tag="IVK93_000731"
     CDS             complement(42931..44262)
                     /locus_tag="IVK93_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6386043.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(44228..45706)
                     /locus_tag="IVK93_000732"
     CDS             complement(44228..45706)
                     /locus_tag="IVK93_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6386044.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(46011..47846)
                     /gene="tdc"
                     /locus_tag="IVK93_000733"
     CDS             complement(46011..47846)
                     /gene="tdc"
                     /locus_tag="IVK93_000733"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6386045.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLEKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT        13699 a        10329 c         8012 g        15933 t
ORIGIN      
        1 ttcaaaagat ttatttactt ttttgtatag atttcgattg ttccacctag tcaatacaaa
       61 tgaggtaatc aggaaactga aaaaaatcaa tacaaaaaag ccgattaaga aatacagcat
      121 tttcttttct gataaaaaag cgtacaagga aaaatccttg gctctttctg gtctcgttaa
      181 tgggaaaaag acccatataa aaggaagaac aacgatcatc catcctaaaa gagccactaa
      241 cgtttgccaa atctttaacc agtaattacc ttttgaaaaa tacttatctt tcaccaaaat
      301 tgatgcaact tcttctcttg ttactttttt catatcctca cctcacgttt ttttgttacg
      361 acgtaggagt ttaccataca tttcatttct tttcacataa agtgttccta aatttttcct
      421 aagaaaaaga acaaaacatt tctcatcagc aaatgaaaaa tattttgttc tttttatcag
      481 ttgttctttt tattttttat ctcttaatta tgttgctctt tccaatacga caatgctttt
      541 cattcctcta gatatcgccg tgtacatttg tctgcgtctt ctttgctcat ctccatcgtt
      601 ttcaaatgga tataagatta catgatcaaa ttccagtcct tttgctagac tcaaaggaat
      661 gatttggcag ttttctaatc catatgaatt ttttttaatc gattcttgta gcgtttgggt
      721 ttcctcttct gtttgggtga taactgccgc tttttctcct tttagagaaa ccaaaatctc
      781 agctagtatt ttcaacaatt cttcagagga tcgaaaagag cggtattcgg gttcttttcc
      841 ttgcggtcgg atcgatacga tgtcgatctt ctcttgatcc acagcatagg ttttgaaaag
      901 ctctgtgatt gctccgctag aacggtaact atttctaaga tcataccgat ggatcggcag
      961 atggtagtca tcgaaacaac gcataatatc agcaaaagta gtggaagaat taaagattgc
     1021 ctgattctcg tcacctacta aagtaaagtg agtttttgga aataattcga tcaatagacc
     1081 taattgtgcc agggtataat cttgtacttc atcaatcaat agatagcgaa atgctggtac
     1141 atttaccttt tcaacaagta aatgacgaat agctaatata atgactgctt catcaacagt
     1201 acgtgtgggt tgccaagcat aagctgagcc atatcgtttt tcataaattc tttcaaacaa
     1261 ctgatgctca tccacccatg ccatttcttc gatttgtctg gttatttttc gatatttttt
     1321 ttgtaaaagt ttccttgcat aagctacaat actttgttcc gaatcatcag aaatcaattt
     1381 accgaataat tccaactgcc tatcttctgt caacgagagt acctgattct gcacagcagg
     1441 actttttgct tgtttcaaaa gatgattctt ccattcgctc atcagtgcct ttttcgttgc
     1501 ttgaagccta tcgatcaatt taggatgagc aggaactttc tcatatattt tttcgatcgt
     1561 ttttttagaa aggactgttt ttccattttt agtaagtcca cggataaaat tcggatgatc
     1621 taaaaacaaa ggatctgatt gttttagatt atcgataaac tttttgctac gcaaacgttc
     1681 tgtctgctcc gatacatttt ctcccgtgat acgtttgaaa taagcctctt caccttctat
     1741 ttcctctgct gaaagctgtg aaaccagctg tatgatggtt agattcagtg gatttctttc
     1801 acctaatgat ggtaggacat tcgaaatata gtcgataaac cgatgattag gtgatagaat
     1861 cagcaagtca tcggatgtca tcgtatctcg atataaatag agcagatacg caatacgctg
     1921 catgatagca gaggttttac cacttcctgc tacgccattt accaagatat tttgactttt
     1981 cacatcccga atgattgtgt tttgttccga ttggatcgta gcagtgatag ccttcatttc
     2041 attggtctca tcttggccta agacttctaa taatacgtca tcttgaatag ctacagacgt
     2101 atcaaaaaac ttgaccaaac gatctctttc caaaagaaat tgtcgtcttt ctttaataac
     2161 cgtatcgatt ttattttgac gaaccgtata agaagatgga ccaatcatgt tattgtaaaa
     2221 aagttcggca attggtgaac gccaatcata aacaagattt tcatctgcat catctgtgaa
     2281 atttgctgta ccgatataaa aggattcttt tggctcatta tccagaaagt ctacaacgac
     2341 ttttccaaag tagggacttt tcaataaacg ttcgatcttt gctagctgat tttctgcgtt
     2401 tttctgtttg atattcatct gatcgatttc tctatttttc atttcaatca tcgaaaaagt
     2461 atccaggtta tctaagtaac tgtcaaaatt caatcgaagc tcgccagcca tttctttcaa
     2521 cgactggttt ccttctattt ttgccgtatc catcgattcc cgcagttcgt tttttttatc
     2581 aattagcttt tgatacacgt tcttcagata tgcttcttct aactgttttt gtgtgttgac
     2641 catacacatt ctcctctttt tttaaagtgg tggaaccgat tagtttacct cttttaagag
     2701 tcgaatgcaa gctttttaac gagtatcatt tgttaggata tatcagttct tcaggtcgga
     2761 cagtttgata taaagaaaat gcaccatcaa ggttttttac gtggtagcca gactgcttca
     2821 agattctttc cgctagatag ctacgtaatc cgctatgaca gctgacgata tattcttgtt
     2881 ggctgtctaa ttcatttaat cggtcccgaa gttcattcaa agggatatgt ttggcttcct
     2941 tgaatttgcc cttttccaat tcttctgctg ttcgcacatc taataaaatt tttccttttg
     3001 ctagttctgt cggcaattca tgccattgta cggtttcact aagcccttct gcaatgttca
     3061 ttgctgcata tcctagcatg tttactggat ctttggctga gccaaatggc ggtgcatacg
     3121 tcaattctaa ttccggtaaa tcgaagatcg tcaaatgccc tttgattgct gttgctaaaa
     3181 tatcgatccg tttatccaca ccttttgccc caacaccttg tgcaccataa atctcgccag
     3241 tctctggatg gaagatcaac ttcagtaaaa tatctgttgc ttctggataa tagcttgcat
     3301 gatcttttcc tgatacatga atgacttcaa aagacagttc gttttctttg gctgttcttt
     3361 cacttaaacc ggtggatgcg gcagtcattc caaacgttcg tataattgct gttccaatac
     3421 cgccttgatt tcttcgagaa attccggaaa tcgtgtccgc aacttgtcta ccttgacgat
     3481 tggctggtga agcaagagaa ataagtgcat cttttccagt gatttgctgt tttacaacga
     3541 tagcatcccc caccgcataa atatcaggaa tattcgtttg ataacgttcg tctactagga
     3601 tacccccgcg tatgcctaat tcgattccag cggtcactgc tagctggtta tcaggacgaa
     3661 caccaatgga taaaataact aaatcagcag ggatggtacg cccatcatcc agtcgcaatc
     3721 gtttcccgtg gtcttcaaat ccagcgacag catgagaagt aatcacttct actccttgat
     3781 gagacaattc tttttctata aaagctgcca tttcttcatc cagtgttgga agaatatgtg
     3841 gtgcttgttc gatcaccatg acttctaaac ctcttctttt taggttttct gccatttcca
     3901 atccgatgaa tcctgcacca acgatcacgg cacgctttgg ctgtttttct aatgcatgca
     3961 tcactttatc gatatctggt acatttctaa tagaaaaaac attgtcggct tctgttatcc
     4021 ctggaatcga tggaacaact ggttttgcac caggagataa aaccaatgca tcgtactgtt
     4081 caatgtgttt ttgaccctca tgtacgactt ctaccgtttt gttttcggga aagatggctg
     4141 tcacttcatg atttggacgc acatctaaac aaaaacgagc ggataatgat tctggcgttt
     4201 ggacaagaag attttcccgt tcactgattt ctcccgaaag ataataaggc aagccgcaat
     4261 ttgcaaaaga aacatacggt cctttttcaa aaacaacgat ttctgcatct tccattaatc
     4321 ggcgaagccg tgtagcagca gacattccac ctgctacacc tccgacaata acgattttca
     4381 tcttatttcc ctcctactgt tcgatcaaac cattgattca tgccaccacg tacattcact
     4441 acatcataac cagatttctt taattcttta gctgcttgtt tactcctcat ccctgattga
     4501 caaatcacat agacggtctt ttctttgtct ccatcatatc tgtttatact ttgtaatgga
     4561 acatttttag ctccttttat atgcccgccc cggtattctg caggtgttct aacatcaagt
     4621 aacgctggat tttccaaaag cttttccttt aattctggga ctgttatcga ttttattgat
     4681 tgaaatagca aattcatttt ctgcacctca tttacatttt tataaatacc tcatggggta
     4741 tattaaacag gagctttatt aatatgtcaa ccagaatgac tcttcatttt tttcactact
     4801 ttttatattt gcataaaata ccgataaggg gtattatcta attataaaaa tagaggagaa
     4861 tgtgttatgg agattgataa taaaaatatc ctgaaccgtt tgaaacgtac agagggacaa
     4921 attcgtggta tccaaaaaat gatcgaagac gaaaaagaat gtatggatgt catcactcaa
     4981 ttaagcgctg ttcgttctag tattgatcgc gtaatgggca tgatcgttgc agataatctg
     5041 aaaaattgct ttgagaaacc tgatagtaat ccagaagaac aagcggctaa actagaacaa
     5101 gcgatcaaga tgattattaa aaagtaaagg aatgatttca atgaaagtcg ttttccatat
     5161 tgatgaatta gaaaaatggt cagaaacagg gaaaaacgta aaaaacctga taaaagcttc
     5221 tccagaaacg acgattgtcg tatctgtaaa tggcatcgcg attaccggtt atctagactc
     5281 tgccaatgca gaattcttag atttgcaagg agtcgttttt cacgcttgcg ccaatgctat
     5341 gcgtgctaat catatttcag aaagttcttt acctgaacaa gtaatcgttg taccagcagg
     5401 tgtccttgat ttagttgaac tgcaatccca aggttatgct tatatcaagc cttagtcagt
     5461 agaggttgcg acagaagtga tcagcttcaa gaaatttcgg cttatttttc gaagaagcta
     5521 cttctggaaa accgtgaatc aaaggttgtg aaaacgctgt cctgcatcaa gtaataagaa
     5581 cagccaaaca aaaatagctt ctcatatttt tcgtttggtt gggcttatta ccgcagatgc
     5641 aagcttttga acaccgttta aggaagaggt tgtgaaatca gcggtttgac ctaagtatta
     5701 gagaaaaaaa cgggaaaata gctcctcata ttttgccgtt ttttgagcta ataccgaagg
     5761 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct accatttaac tccgaatcac
     5821 aagaaataat ttcaaaaaca acttctcctg tttttggttt ggttggctta ttatcgagaa
     5881 gtagctactg ggagtctatt tatcaaaaaa aggaatgcga cataggtcac accccttttt
     5941 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
     6001 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
     6061 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
     6121 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
     6181 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
     6241 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
     6301 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
     6361 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
     6421 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
     6481 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
     6541 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacagc cactgatatt
     6601 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
     6661 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
     6721 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
     6781 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
     6841 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
     6901 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctggcgttct tttttattct
     6961 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
     7021 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
     7081 caatccacaa agcttggctt tctcctgttt ctccaacccg caactgatat ttaggacgaa
     7141 gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt
     7201 tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt
     7261 ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat
     7321 ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat
     7381 gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa
     7441 tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg
     7501 ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc
     7561 cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct
     7621 caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag
     7681 gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg
     7741 tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg
     7801 ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat
     7861 tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga
     7921 taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc
     7981 ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt
     8041 tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca
     8101 aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt
     8161 ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt
     8221 gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga
     8281 tcaaatcggt tggctgcaga atcgaccctt ttttgacttg ttctgtcagt gcatcgatcc
     8341 tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg
     8401 tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc
     8461 gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt
     8521 gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc
     8581 caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg
     8641 tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac
     8701 accaaataaa ctttgtttat tggctattcg acttactgat tagataccag ctacactcac
     8761 aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag caagacacat
     8821 tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat agacacctac
     8881 cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga
     8941 agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc
     9001 agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc
     9061 acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc
     9121 aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat
     9181 agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg
     9241 tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat
     9301 gatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac
     9361 tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc
     9421 gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa
     9481 attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc
     9541 ccccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc
     9601 tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca
     9661 ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg
     9721 ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc
     9781 ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc
     9841 catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata
     9901 agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt
     9961 ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat
    10021 ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc
    10081 ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg
    10141 ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt
    10201 ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc
    10261 acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc
    10321 attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc
    10381 gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata
    10441 atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt
    10501 cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt
    10561 tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg
    10621 gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac
    10681 tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc
    10741 atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc
    10801 aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc
    10861 aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc
    10921 ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc
    10981 atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga
    11041 gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg
    11101 ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac
    11161 tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg
    11221 agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc
    11281 tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa
    11341 ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat
    11401 ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt
    11461 tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc
    11521 ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta
    11581 gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga
    11641 attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga
    11701 ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta
    11761 aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc
    11821 gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct
    11881 catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt
    11941 gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag
    12001 catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata
    12061 attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga
    12121 ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc
    12181 ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg
    12241 agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct
    12301 gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag
    12361 taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca
    12421 tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta
    12481 tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt
    12541 gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa
    12601 ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt
    12661 tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg
    12721 caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt
    12781 gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa
    12841 agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg
    12901 tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta tcatactact
    12961 gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta
    13021 ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt cttcttttca
    13081 atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt
    13141 ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct
    13201 caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa
    13261 tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca
    13321 tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt
    13381 tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat
    13441 gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc
    13501 caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt
    13561 ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat
    13621 ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt
    13681 cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca
    13741 gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc
    13801 ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca
    13861 atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa
    13921 acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt
    13981 gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc
    14041 gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta
    14101 agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg
    14161 tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact
    14221 tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca
    14281 caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa
    14341 tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc
    14401 tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct
    14461 gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt
    14521 gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac
    14581 tgttcaaacg tccatccttc ttttactgca attgtcagtt ggtcaagaag gtggaacatc
    14641 tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg
    14701 agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt
    14761 ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca
    14821 aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt
    14881 gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt
    14941 tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga
    15001 gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct
    15061 tcccacatct gattgtcggg gcgagaatga ttagccagca aaacaagatc atagttctcc
    15121 tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct
    15181 tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca
    15241 ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt
    15301 cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt
    15361 ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg
    15421 tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga
    15481 atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat
    15541 cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc
    15601 gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg
    15661 ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc
    15721 aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg
    15781 ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca
    15841 cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc
    15901 tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga
    15961 gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct
    16021 tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt
    16081 gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac
    16141 tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat
    16201 ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc
    16261 atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact
    16321 gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa
    16381 caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga
    16441 agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc
    16501 ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa
    16561 ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa
    16621 aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat
    16681 cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg
    16741 acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc
    16801 atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg
    16861 cttggcaaat aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca
    16921 cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct
    16981 gcaagttctg catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca
    17041 ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg
    17101 ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct
    17161 agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt
    17221 gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt
    17281 tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag
    17341 ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa
    17401 tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg
    17461 gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt
    17521 tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa
    17581 gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct
    17641 ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg
    17701 agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca
    17761 ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg
    17821 aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct
    17881 gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct
    17941 gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa
    18001 tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc
    18061 taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca
    18121 gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa
    18181 gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc
    18241 aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt
    18301 ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc
    18361 ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc
    18421 atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata
    18481 gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt
    18541 tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct
    18601 ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt
    18661 ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt
    18721 ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca
    18781 gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct
    18841 gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag
    18901 caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg
    18961 gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc
    19021 ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa
    19081 atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga
    19141 tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc
    19201 acgacgtata accaaataaa tctactccat cttcgatagc ttccttcatc tgctcaatat
    19261 gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt
    19321 ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt
    19381 gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa
    19441 gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc aaattttctg
    19501 gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg
    19561 ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca
    19621 ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag
    19681 ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa
    19741 gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg
    19801 tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat
    19861 ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga
    19921 tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag
    19981 cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca
    20041 aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac
    20101 attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac
    20161 catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag
    20221 caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat
    20281 aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc
    20341 attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag
    20401 ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc
    20461 cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct
    20521 tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac
    20581 aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat
    20641 caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc
    20701 atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac
    20761 taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta caggactgta
    20821 gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt
    20881 tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat
    20941 atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca
    21001 ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata
    21061 aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc
    21121 agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt
    21181 aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga
    21241 taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc
    21301 tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga
    21361 aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg
    21421 ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa cagctaaata
    21481 tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta ataaagtaat
    21541 tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa
    21601 gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat
    21661 aaccgtactt gtatatcttg ctagaatcac gggcatccct agaaatcgca aggttatgct
    21721 atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat
    21781 gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata
    21841 gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa
    21901 attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc
    21961 taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt
    22021 ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac
    22081 gtcatacact tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac
    22141 tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt
    22201 tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc
    22261 gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt
    22321 aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc
    22381 cattcaatta gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac
    22441 ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc
    22501 aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag
    22561 ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc
    22621 ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc
    22681 tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt
    22741 tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctccgaaga
    22801 ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc
    22861 ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag
    22921 atacactggt tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt
    22981 ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt
    23041 tcgaaaagga ttcaacgctt cggcaagata caaaaccaaa taagcgcttt cagctaaatt
    23101 gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata
    23161 acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt
    23221 tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg
    23281 aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt
    23341 gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg
    23401 tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc
    23461 taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag
    23521 aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt
    23581 atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat
    23641 tgtactgatt tgttgaggca accttgcgtg ttggatcacc gtttctgttc caacaagaga
    23701 taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac
    23761 ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga
    23821 tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt
    23881 ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt
    23941 atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa
    24001 ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt
    24061 tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct
    24121 tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa
    24181 gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca
    24241 atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac
    24301 cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc
    24361 ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc
    24421 ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac
    24481 catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg
    24541 gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta
    24601 tgaataatgg taattcataa cggtctgtca attcattcat gacataacga aaccctagag
    24661 gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac
    24721 catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat
    24781 aataactaaa tgcaatataa tctaccgtgt tagcttgcaa gtactgttcg tctccatcta
    24841 acattactgg tgcacagtct tcttccttcc atatcctgta aatataagct ggataatgcc
    24901 ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata
    24961 cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac
    25021 ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa
    25081 acatatgatg acaggcttga taaatctctt tttttgttag tgtagcaatg ggttcaagtt
    25141 tatctctagc agggaatgta accaatccag cagctgcata aggcatacga aaagcattat
    25201 tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct
    25261 gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta
    25321 ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat
    25381 ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt
    25441 ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact
    25501 ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa
    25561 gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa
    25621 tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt
    25681 cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt
    25741 ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa
    25801 gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa
    25861 attgaatgct actcttcatt tttaatattt atatataaca aatacataag acgatccccg
    25921 aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct
    25981 tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca
    26041 ataaccctta aagcttcacg tggaacagtg aacgtctgct tttcactgga catattacaa
    26101 ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc
    26161 ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc
    26221 aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt
    26281 tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca
    26341 cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcatt gattgattcc
    26401 aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca
    26461 atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct
    26521 tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt
    26581 tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta
    26641 ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt ggataatact
    26701 tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga
    26761 tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa
    26821 taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg
    26881 tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt
    26941 gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccacca
    27001 atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt
    27061 ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt
    27121 tcatccaact gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca
    27181 ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc
    27241 tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg
    27301 atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct
    27361 gccatgattg cttgataatt actgatatca tacccattgt catcattcgg agactcgtat
    27421 actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata
    27481 atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt
    27541 tgataaacaa ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat
    27601 gtttctattt tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt
    27661 caaatagcta ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa
    27721 ttccctcaac tgttttttga aaggattgat ttattcaggg aagaatgctg atttcacaac
    27781 ttctttataa acggtgttca ctcagctgtc cctcggtatt agctcaaaaa acggcaaaat
    27841 ataagaaact atttttttcg tttttttctc taatactcag gccagaacac tgatttcaca
    27901 acctctttat aaacggtgtt cactcagctg tcccttggta ttagctcaaa aaaaggcaaa
    27961 atatgaggaa ctattttttc gtttttttct ctaatactca ggccagaaca ctgatttcac
    28021 aacctcttta tttcacattc aatatccaca tgaatcccat gaatacaaag aacagaccat
    28081 acatgatggg agatacttct tttcctcttt tagctgctaa catggtaatt ggatagaaga
    28141 taaaacctaa tgcaatccca tctgaaatac tataagtcaa aggcatgccg atcaagatca
    28201 agaatgaagg gatcgcaatc tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca
    28261 tcaatactcc taccataatc aatgctggtg cagttacttg tgaagtaaca actgatagta
    28321 atggagaaaa gaacaagcct aacaagaaga aaatccctgt agtaatagct gtgatacctg
    28381 aacgtcctcc aacggcgata ccagctgatg attctacaaa tgcaccaaca ggagaagtac
    28441 ctaataagga acccgcaagc atagctgttg aatcagaagc taaagctttc cctacacgag
    28501 gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa tccaactaaa gttcctgctg
    28561 tatcaaagaa agtcacaagc aagaatgtca gcactacgac ccacatttga agagaattga
    28621 tgtcccctac atgttttaat gcaactaaaa atgttggttt caaacttggt gctgcggcaa
    28681 caatttcaga aggcattgga atcaaacctg tgaccagacc taaaatcgtt gtagctgcca
    28741 tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa gatagcagtt acgactaatc
    28801 caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc taatcctact aatgtagatt
    28861 cattcgaaac gatgatccca ccttcgctca gaccaaggaa agcaataaat agaccgatac
    28921 cgccagaaat cgcatatttc aaatctgctg ggatcgcatc aatgatcatt tcccgcagct
    28981 tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa tacgccggct aatgctgttt
    29041 cccatggaat ccccattcct aaacaaacag aataagcaaa aaaagcattg atcccaagag
    29101 caggtgccgt tccaattgga taacgggcta atacccccat taaaatacag ccgatcgcac
    29161 tggctaatgc agtcgcagta aacactgctc cttcatccat tccagaagca cctaatacag
    29221 ttggatttac aaataagata taagccattg agataaatgt tgtaaatcct gccagtacct
    29281 cacgttttac cgtggtgttc aatttttcta tttcgaagta ggaaagtatt ttttccttca
    29341 tcttccatcc ccctaaatcc gaacattata aataaagtcc gttaaaattt cgatttatta
    29401 ataaaacaac gtgttttatc atatactaaa acaaaacata agtaaacaaa aaattcggat
    29461 tttaagcaaa atccgaacgt tccatctgat taaatccctt tatctcaaag ttcatgaaca
    29521 ttcactttgg attacaagaa tttctttatt gtgttaataa agtttcttca ttctagtctc
    29581 aaaaatcaat agtgtgattt gattttacca tttcgtttct gttcttcttt gatgaattct
    29641 tttgtatcga caggattcag attatttagt ccgccaaaac ctgtatcgac catttttccc
    29701 cagccttttg tcttcttttt tcctgatttg agattctttg ctcgaagctt ctcactatgt
    29761 tttgctcgcc gatacttcaa ataaccttct aatcttccca ttagatccac tgtttctcct
    29821 cctttttatt caatcagacg actcagcttt tcctataaca aaataattgc cttcagggtc
    29881 cgaaaaattg aaagtcaaga cgccattgtt gtctgtgatt tctcctggtt cttccatgtt
    29941 ctcgtagata gtttcaaatt catccgtaaa aaatactaaa gaaggcggtg gtcctaatac
    30001 ctctggtgaa aacttctgca caaatacttt tgggaatacg cctaattcta attcttttga
    30061 gatgctgagt acaatactct taaattcttc tggcaattcg attgtttcag aaatttctgc
    30121 accaattttt tctacaaaga atcggcaaat catctctacg tcattgacat aaagcatcac
    30181 acgtgttttt tgtatcatcg tttgaccctt ctttctattt gattttcttt cctgtctata
    30241 aactgattcc attatatcac gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa
    30301 ataaagcaat agtttctagg attgctttcc tccacaaact ttttttacaa aaaaaaaacg
    30361 gcaatgaatg ccgctcttct tatttcgatt cttttataat tctgcttctg attcatcttt
    30421 ggttagtgtg tgtctaacac tagttacgcc aaatgtttga tcgatttttt ccttcaacgc
    30481 ttcgattgcc tgtgcgccac gtgccgcttg ttcagcattg atggattcaa tgaccagcac
    30541 cgctgcttgt gtttcttccg tcagcatcgt tctttgggct tcacgccagt tgagacagcg
    30601 acagatcgct ccttgttcat catagtagat gatttcttcc ggaagtgccg gagcatcgga
    30661 atctgcacct aaaggcagga atgattcgtc accttgggct tttcctaaat gcagatttcc
    30721 tgcgatcttt tcgatatctt ctccgccgca aggtacgcca aaagacaagg aaatactatt
    30781 atagatatct acaagcggat tgattgggaa aaattcccgt tcttgatttg cacgtttcag
    30841 taaagcttca atagaagaac gtgcaccttt tttagtcttg aatgtggtaa atgcctgacg
    30901 ccactgagca atgacttcgt tttggctgaa tgtttcttct gtaaggtaat ttcttgcttc
    30961 ttttgctgcc tcattcaata attgatgata cgtttcttca ctttcttttg ttacgtggtt
    31021 atcgatacca tttacggata agatttcgat ggttgcttca ggaaatactt cccagaaatt
    31081 ggtatccact attacttttt tcatctgttc acacctcttg attagaataa ttgcttgatg
    31141 aatagatagt ctgtttgggg atcatctccc atataaaaaa catgttcgct gtatttgata
    31201 aatccttttt tacgataaaa agcgagcgca acatcgttgt gttcccaaac accgagccaa
    31261 atggctgttt tttgcttttc ttttgctttt tcaatagctg tatctagtaa aaatgtaccg
    31321 ataccttgat gtttaaattt ttttcgtaca tagattcgct cgatttctaa aaaatgatca
    31381 gccatttcct cagattgact cgttcctgta tttagtttca ggtaacccgc aggctcttga
    31441 tcgatactca ctaggaaaaa atctgtttct ttatctttta gctcttctgt aagtttttct
    31501 agtgcataag cttgtgtcaa gtaatcagct aaatgctccg gtaattatct tttccaaatg
    31561 tatctgtaaa tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt
    31621 ttcgaatttc gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt
    31681 tgacaaaatt ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt
    31741 gttttgcttc ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc
    31801 gaatgatgaa aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt
    31861 tattttcttt atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc
    31921 gggcagtcgt tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag
    31981 gattttcgca aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga
    32041 attcgatatt gctaatagag tctagcgctc gggcaatcat tccgatgtct cgaaggattt
    32101 cagccatttc atcccacctt tattttgttt gtaagactag tttacctctt tttgttgcaa
    32161 atgcaactat ttttaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct
    32221 tttcttcttt tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag
    32281 taactgtttc ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa
    32341 tcccatgttg gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca
    32401 agcaaaaaca agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc
    32461 aaaaactgct cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt
    32521 acgcacattt tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc
    32581 tttcgtccgt ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac
    32641 atctactttt ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgaa
    32701 agtttctttt cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg
    32761 ctctttatca aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct
    32821 tttgtattga gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact
    32881 atgtaatcaa gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa
    32941 taaataaaag aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg
    33001 aataaatggc gttcaaaagc ttgcatctgc ggtaataagc cccatcaaac gaaaaatatg
    33061 acgagctatt cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct
    33121 ccccactaaa cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac
    33181 atgagaagct gtttctttga aattgctcct tgtgactcgg agctgaacga caggctcacg
    33241 acctctcggt aaacggtgtt caaaagccaa tttttcgata ttaagccatt tctttgttac
    33301 ttattgtcat tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta
    33361 tagtctgtta catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg
    33421 gcgatgatct ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc
    33481 aatacgtcac catagtgata atgatccatg atatagctca aatgacgatc tgattcacga
    33541 tcacgtttgt accgacgttg ccataacact tcaaagtctg ctgttaggcg aatcgtgatc
    33601 acctgatatc cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa
    33661 ggatactcgg acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa
    33721 tagaaaggat aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg
    33781 atggattctg catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat
    33841 cgttcccgta gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga
    33901 atgaaatact ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa
    33961 cagtccaatc ttatgcttga caatttcttt tactgcttta tttgctggaa tcacattatg
    34021 gcgaagagac ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt
    34081 gactaaaagc tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata
    34141 atcttcttct ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc
    34201 gatttctttc agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt
    34261 accgatagca atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc
    34321 cggtcttgta tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat
    34381 tgttccaagt tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt
    34441 tgtcttcaag atattttctt tgaaaggtag atgggacccg tcatacatga cagaagtata
    34501 tccgctatcg attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgtaagac
    34561 tgcatcaacc atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc
    34621 gatatattta gcggtatcga cacttgtttg aataatgatt ggcgaaccca tttcttttgc
    34681 tgcgcctagc atggcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa
    34741 attcaactct tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca
    34801 ccttatcttt ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat
    34861 ccattttgcc catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac
    34921 gcttctggtt atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt
    34981 ccaagctctt ttctaaatat tggatggctt gatcgttctc atgcaattca gaaaatttaa
    35041 gtccaacttg ttcataaagt tccgctaatt tatgcctatc ttgggtagtg gaaatctctt
    35101 gtttcaattg tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt
    35161 cttcttttgc cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca
    35221 ttttccctac tttcaattaa aacgctttca aaactgggct taagatccag gcatataaga
    35281 gtgctgcggc tactgtatct acatcggttg ctgtagcatt gatgaatccc atcgaattaa
    35341 agatcgttac tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa
    35401 tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa
    35461 agaaattggt tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca
    35521 tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa aatgacggca tttgggctat
    35581 aaggaaagaa aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta
    35641 tccctcgaaa tgccggcacg atttcaccta acagtagacg tacaccagct agcaatatat
    35701 acacacccac tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc
    35761 ctgataattc agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga
    35821 ccatcacgac catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact
    35881 tgatatcttc gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg
    35941 cttcaaataa gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga
    36001 ttttacgaat gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa
    36061 aaccaccgac caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg
    36121 tcatcgttgc catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga
    36181 aacgagccaa aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac
    36241 ctaaacccaa atcattcatt gcttgatcat tgatagattc aatagaaggg atgatccctt
    36301 gcatatgaaa tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc
    36361 tcgaaccggc actcaatact tgaaatccta ggagtgtttt taacgtgcca gagatgactt
    36421 gtcctgctga tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg
    36481 atgcctgagt caaaatattt tcgatgataa aattgataac gctcatgttt ttaccctctt
    36541 ttctttttgt atttagataa ctcccttatc ttgcagaact ggggtgattt tttcttcaat
    36601 ttcatttttt gaaacaatat ttttcaaata gatcatcgcc gttttaggat caactgcgaa
    36661 cttttcgaac tgcgaacgaa aattttctgc tgtgatgatc acatctggtt tcatggaaac
    36721 tgcggaagaa atatcacaat ggtccagttt ggcttctact cctaatttgt tcaaaacatc
    36781 ctcagcagac atctgtgctg caaaactact tcctaaacct gcgccgcata cgaataaaat
    36841 gttcaatttg ttcatctttg ttctcccccg tatcatttat tatttctcaa ctactgaaaa
    36901 aagcagttgt ttgaaatctt cgatatttgt gcattcaatc aatcgatcca acttctcttc
    36961 gttgttgatc aattcaatta gttctttcat gatgttcaga tgggagtagg agtctactgc
    37021 tgccaaacaa aaaataatct ttactggatc catttcagga ttaccgaaat cgattggttg
    37081 ctcaatcgta gctacgctga ctcctaattt attgacccca tcttctggtc gggcatgggc
    37141 tagtgccatg tgtttgccaa tcacgatata cggtccgtaa tcattcactg cacgaatcat
    37201 cgcttcgaca tagcgctctt cgacaatgtt ttctttaacc aatggtctag cagctagttc
    37261 aatcgattcc tcccaagtag tggctttttc tttgatcatg atattgctgt ccgtcaaaat
    37321 atcttttaac ataggctgga tctccctttt gttgatttct aaattgtttg tttcgaataa
    37381 tttttctaac ttgccataga tgattcgatc tactcggcca ccactttctt tcatgacatc
    37441 tattatttga gtgaataact ccgtcgattc atgactgtta ggctctaacc gctgtagttc
    37501 ttcatttctt ttcaaaaagt cttctataac caagatattc gtctcagtta aaatcggatc
    37561 gacaactaaa agcggcttat ttcgataatt caattgaaag gtgctgaaaa ccagattgac
    37621 atccaattta tctactaatt cgatttcttt tgaacttaaa actgcagtaa tctcgatctg
    37681 aggaaacttt tctttcagat tctctgctaa cagatttcca gtagccatcc catggttaca
    37741 gacaacgact gatttataaa cataattcaa gtctcgcttg attacactgg cgatcgtcga
    37801 aaagtggatg acaagaaaag ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc
    37861 gatcgtagga acaaaatgag tgactgcgcg gtagattggg aaataatttg ttcggatgtt
    37921 ttctttcaat ggattcatga cttggatatg attcttgatt cttacaatca gtgcagccat
    37981 gtgcttatag agattttcga ataaggcttc ttcctttaga tggaaaggta cgtgtgtctc
    38041 gttctctaca aattggatca attggatcgt tagtaattga gcttgtaccc actcgattga
    38101 attggagata tctctagcat tgaacgtctc aatagtgaaa cgaagataat ttctttctac
    38161 ttgcgggaca ttgtccaatt gaaattcctc gatcttcttg tttcaacaac ttattggaat
    38221 agatctcctg tatttttttg atttcctcca aaggaaaata atggagaaag attttttgag
    38281 ccggtgttaa ttgtgcatct tgattcataa agaagtcggt cattcccaga ttctgattga
    38341 taaaatcata gatcatcgta cggatagatc gttctgaacc tttataaacc agtccttgct
    38401 ttccataact gacgatctct atcccataac tgaccagtgt tgctcgtaac cgtctcatat
    38461 cttcatctaa tgtactttta gagatttgaa attcttcttc ttttttgttt agatggatcg
    38521 ggattcggct gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc cgattcaaaa
    38581 agccgtcttt cacatctttt atttcagttt ctaatcgagc ttgcttttcc ttttccagct
    38641 tcaacaaaaa acctttgttg cgaattgtac ggatctccgg aagcttttta ctgctcaacc
    38701 agatattcgc ttcaagaact tcctgacgaa tcgtccgctg actgacattg aactcacttg
    38761 ctagatgttt tgcttttact ggatattggg ctgtcatgat tctgtgaagt atttcaattg
    38821 ttcttgtttt catagaaatg atctccttct gatttcaagt atatggttcc gcttacataa
    38881 taaaaagtag atatttctgc cacttgaagt ggaaagaaag taagcaaagc aaacaaaaaa
    38941 taagccatat gctttttcat ctcctcaacg aaaaagacat acgcctatta ttacaaaaat
    39001 tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
    39061 caactaaact aaaatagtgc ttcctatttt ttgtttggtt gttcttatta tcgcagatgc
    39121 aagcttttaa acaccattta gtggagaagc tgtgacagag atgttcagcc ttgagcgata
    39181 agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta ttgccgatag
    39241 gctatctctg gaacaccgtt tattcagaaa aaggagctgc gacaaggctt ttgtcacaac
    39301 tccgttcata aatatttcct gtcaatccag cataatcggt tgaataaaat cggcttgcca
    39361 tcttttttct ttctcagaaa aatcaaaata ccatatctga gaaaaatact gtcgttgtgt
    39421 cttacttcct gagatcaatc gcccatcttt tcgatcgact gaataatcac agcatctgaa
    39481 atcaattcgg atggcaaaac tattttcgtg gatctgtcga taatttccta cttcctgaat
    39541 agttattttt ttagtatgat ttcttacacc gtctgctttg tttttttgca gaactttctg
    39601 atgttcggca aacagcttgt ctgtataaca gcttctggct ttttcaggag tttcttcttc
    39661 ccaagcggat tggatcttca tgaatatctt ttggatttcc aaaaactgtg ctttcttttc
    39721 tttagccgtt cctgacatgc gggacaatat ctccgatttc agccttttct tttgtctttt
    39781 attcttgacg atagaaaaag cagaaacccc tcctactgac aaaacagcta atgcaagaaa
    39841 ccccgtttgc tcataactag aaggacctgt acttggacca taatacccat aagaatctct
    39901 gccgtaagaa cgactgccaa aataactgcc cccatatgaa cgaccgccaa atgaacctct
    39961 gcccgaactt cgaccgctag atgtacctcc accatgacca cccgcgacag cgaatccttg
    40021 ggtcggcaga gcaaacaaac ataggcccac taacaaaatg attgctaaat aattgatttt
    40081 ttcattgtct tcctcgttat cttttattac aaaaaactat ttttatcata gccttgctaa
    40141 aaggaagagt caataaccag atgcataaac tgttttttta gcaattttgt cttatttagt
    40201 cattgagtaa catcggacag atgtatgtgt cggcagtcca taattcctct atcttcagca
    40261 tgaacaagct tgcagacaag agatctgcgc ttcctccagg agagatttgg ttttcctgac
    40321 aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc actttgacta gcagcatgag
    40381 caagtttttg catttccact aacgttttct gatcactgcg atgcaatacg catgtgtcat
    40441 ctagcgaact catcaaaact aacaacatat gcatttgttt ctgtacatat gaatcattat
    40501 ctagctcctt agctgcttgg attgcaagcc tgatatgcgg ataccctaat atcgcttctt
    40561 catatgcacc atttacgcca tatttttgct tcaccctatg accatgagtg gtttttttat
    40621 gacgatattt tgtatcagga aaagcagcaa gcttgctgac atcatcaagt attttcgata
    40681 aagaaaaagg ctgttccata ctgatttttt gtgcaatgac actgatcaat agccccattg
    40741 cccaaatcgc tcctttatgg gtattgatgc catttgtagc aatcagcatt gccttttccg
    40801 cttttcgccc aatctcagca attctttctc ttaactcctg atcaatagca taatgccatg
    40861 ctatttgagc catttcttca aaaaaaggag ttagcgtctt agcagactta tccaaaatgc
    40921 gccgaccttc ttttacggct tttgcgatcg tatggaaatt atcatctcct aaaaccatat
    40981 cagctgcctg tttgcttact tcactacctt tgattcccat agcgattcct atatcggctc
    41041 ttttcaaagc aggtgcatca tttacgccgt ctccggtcat tccgacgatt tctccatttt
    41101 tctgcaaagc tttcacgatt cgcagtttgt gttctggtgt cgttcgagca aagacgtcga
    41161 cttttggtac ttgtcgaatc aactcttctt cagacataag atccaaatcg atcccttcta
    41221 aaactttagc agtatgtttt aagccaattt gttccgcgat cgcttttgct gtatctttgt
    41281 gatcccccgt aatcattttc actgagattc ctgcttgttg gcattccttt acagctttga
    41341 ctgcactttc ttttggcgga tcaatcaacc cagcaatccc tgcaaaagtt aattgggtta
    41401 aagactcatg cgtgagttcc tgtttcgaat ctactttttt gtaagcaaat cctaaaacac
    41461 gctgaccttt tttcgcaaac gctgtggctg tttgacccca tgcttcttat tctgaagaag
    41521 aaagagttga tttagccaat aatacttctg gcgcaccttt gacgtaaatc acccctcctt
    41581 tttcctccgc atgcatcgtt gccatatatt tgtattcaga gctgaacgga atttttgctt
    41641 ctattttttt caaaggcaga tcggctgttt cggtgtggcg aatcagtgca agttctgtcg
    41701 gattgccact taaattttct gttttctgtt cttctttcgt ctttaattcc tgacaatttt
    41761 tcataataaa cagtacgcga gtttcttcct ttgctgctac atccacaact gtcatttcat
    41821 tttttgtcaa tgtccctgtc ttatctgaac agatcactgt catagaacct aatgtttcta
    41881 cagaaggcag tcctttgatg atcgcctttt cttttgccat ttcatgtact ccgacagata
    41941 ggatcatggt caacacagct ggcaatcctt cgggtaccat tgacaccacc aaagcgatca
    42001 tagctgaaag aaggatatgc caatccatgc catagcggaa tgttgtaaaa acaactaaga
    42061 acaagatcaa acaaagaatg ccttgaaaaa tttgtttgtt caatcggttg atcttcttga
    42121 tcagaggcgt cgtttgttgt tcgaccgatt gcagtgcttg attgatttta ccgatttctg
    42181 tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc agattgtaca agcgttcctg
    42241 aaaaagccat atttttttgg tcaccgattg attcttctgc tgccaaaacg gaatgatttt
    42301 tttcagatgc ttctgattct cccgtcaaaa tcgattcttc tactagtaga tcatgtactt
    42361 cgatcaaccg aacatctgct ggaacgacgt ctccggcacg taagacaatg acatctcctt
    42421 ttactagttc tttcgcaggg attttttctt gcatcccgtt cgatagtact atcgcttctt
    42481 gtcccatcag ctgcttcaaa ccattcaacg attcttctgc ttttctttct tgccaataac
    42541 taacgagtcc gttgacgatc actacgaata aaataatact tccttcgatg tattcgctcg
    42601 aagcaaattt caaaaaagag gcaatgatca acacgatcat cagcagatca gtaaagtgtt
    42661 ttgctatttt tttccatcgt tttgtctgct gtcttgcttc tatctcatta tatccttgat
    42721 cagtcaggcg tttctgccgt tcttcttgcg acaagccttc tgttgatgtt tgatagtttt
    42781 tcaatacatc ttctaccgtc aatctgtacc agttcatcct atcccctcct gtttaattga
    42841 tactgttatt atacgcaggg aagttttttc aggcgtatcg attatttcag gggggtctga
    42901 aataattcgt tttgcagaat gtatctgggc ctaactgata tttctattgc ttattctttt
    42961 atatacaaaa tttaagaata caaaccagat aaccaataaa aaaggaataa agcgtgtcgt
    43021 gctttcgaat aacaagctga aagcaattga agtaaaaaac accaacaaag caagatcttt
    43081 atatctttgt gtagtgttcc gtttcttttc tttaataaat tgaacatgtg cacacaagat
    43141 cgaagaccac acaaatagaa aacaactcgt tgtcatgcct gccaaaagct gaaaaaacaa
    43201 tcgactatca gctacaaaaa aatgtagaca aagaccgaca aaaaataaca tccctgtaat
    43261 taaaataccg tgtaaaggta cgcctttctt agaaagcgca caaaaccaat ggggcaaact
    43321 tttctctttt gctgtttgat ataaaatcct gctagtactg tagactgcac tatttgctga
    43381 ggacaaagaa gcaaccaaaa tcattagatt gacaattgct gctccgcttt ggcaatggat
    43441 cacttgcagc atagtcacta aaggactttc ttttaaatct aaacaatgcc atggaacaac
    43501 aaataaaatc gacaaaatcg ttcctatata gaaaaagagg atacgccaag gcagacagtt
    43561 gattgcattt ttcaaattct tttgtgggtc actcgtctca ccaactgtta atccgatcat
    43621 ttcaattcca gcaaaactga ataaaaccat aggaaatgaa gcaatcattc cagcaaagcc
    43681 aaacgggact aattcattga gctgtccaaa atttgctttg attccaatag actgggtatg
    43741 aaaatctgag acactcaatc ctatacccaa taagataaaa aggaaaatcc ccaagatctt
    43801 gatgattgag aaaaaagatt ccaattcacc aaaccacttg actgcaacaa ggttgactgc
    43861 cattaagatc attagtgcgc tacctccgct tatcaaagct ggtacatgta caaaccagaa
    43921 atggatatac aatcctattg ctgttaattc cgcaagtcca gctccaagcc agcaaagcca
    43981 ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa taatagtcaa cgaaatcact
    44041 gatagagtta aatgtactat ttgctaaaaa taaatcaccc atgacccgca tcaaaccata
    44101 aatagccaaa ccgcatatca aataaacgag aataacagct ggccctgctg atgcaattgc
    44161 ctttgctgaa cctaaaaata atcctgttcc aattgcgcca cctaatgcga ttaactggat
    44221 atttcgatta gttagagtcc gtttcattgc cggtttttcc atcaagttca cctttttctt
    44281 tttcaagcag catggcgact tcctgttccc aagaaggttt ctttattttt tcaatacaga
    44341 gtggtactac aaacaaaatg acactgatgg ctgtgactgc aattagattg atccatgtcc
    44401 cagctttcat cgtcagtccg acagaaaatc cgattgccgc aaataaaaca ataacgacag
    44461 tccaaactcc aagattgctc tttttctctt tttttccaat tctaaatggt cgattcaact
    44521 ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat gatgacatag acgatacagt
    44581 agataacagt agttgaattt acgagcatca aaaatgcttg attgacgcca ggaatcagca
    44641 aataaaaaag tgcaaagatt gagataatga cagactgagt caaaataacg gatttggata
    44701 cgtcaaattg attcgtcttc caaaaattga attttggtgg atacgcacct tttctagcac
    44761 tttctgtcac tgtctttgct ggtcctgagg cccaagcaga taattggact gctacaccaa
    44821 tgaagacaag taaactaaac agattcacaa taagatgtgg caaaccaaga atttggcaat
    44881 aaatcgaaat agattgcgcg atattattca attccatttg tccttttggt acgacatttg
    44941 ctacaactaa agcattcaaa atattgacga aaaatataaa aattaacgcg gcaaatactc
    45001 cgcgaatata cgttttaact ggatttttca accgagtgat atacacagag gacatttcga
    45061 taccagtaaa aataaacatg atcggcgcaa aataaacaaa tgaggatgca gtcgtagtat
    45121 ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc ggttggtacg atcccaactt
    45181 tgatccacgc agcaaaccct aataagagca tcatcgtcaa aggcacgtac aatcctaacc
    45241 aaacaccgac ttttccgccg atttttgcca tatcaaattt caaattcaaa aaagtgatga
    45301 tccaataaac gactaaaatg acaactaatg taaatttgct gttcaaacca agcggcacat
    45361 tatcgataat attaccaaac aacggagcaa gtgctgaagc gaccatcacc atcccaggaa
    45421 acatttggac ccataacagc catgagacaa cgaaccccca tttttttcct agagcattcg
    45481 atacccaaag ttctggacca cctttttggg gaaacattcc ggcaaattca ccagagatca
    45541 atgagattgg aaaagcgtac aataatactg caaccagcat gtaaaaaatc atcgtccagc
    45601 tcgttgctgc tacatctgga atatttcgta cacttgctac aagggcgcag gtcattccga
    45661 taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc tttcatcttt attccttctt
    45721 tcaatagcga catatgtttt tgttttcttt tatttttgtt caagaacaga aatgacaagg
    45781 tttcatcgat tcagtacaaa acccagtatc accgaaacat ccattgaacg atcttagtga
    45841 actgggtttg atacatgaaa cgaacgatcc aacttataga acgttttata aaaaagaata
    45901 tcttggcgag gaatatatga aaaacagggg gaaatacaat tgttttgatg aagcaaataa
    45961 ttgtctgttc tccatatgtc tcttgttatt ctttcacaca aaaatcagtt ctattggatg
    46021 atttcgttta gtttcttttc cattgctttg gcgatttctt ttgtataaaa attgaaaatt
    46081 cgatcatcat tcaaataagg agtcatgata gccgcacgta gcagagtgat tttctgttct
    46141 tttttccatt cttctttcgt tagacccatt ctttcaacaa aacgaatcgg agaatcaccg
    46201 taatcttctt gtgtaaatgt agtatgagaa gtaataaacc gttcaccata aacatcgcca
    46261 tccatataag aagaccgatc gaacattttt tcatttaatt cattgatagc gttcaaatct
    46321 gtacagcctt gttctttgaa tacccaattg accatattaa aatcaggatg attcaatgga
    46381 tagacttcga ttgtttttcc tttaacagta aaagtcagat gatcgagaaa ttctctgaat
    46441 ctttgagctg cctcgattga agccccaatc aattgaccat atccggtcac attcaatggc
    46501 aatacacgat gtgctgtcca gactgcggcg gcggttgctc cagcttttga tccttccaaa
    46561 atataagctc ctaacatatc tggagccttt actgattttt caaacacata tggcgcgaaa
    46621 taagaaatga tatttctcat atttttatgt ttgattacga ttcctccagc agcataaggg
    46681 acatagccca tcttatgagg atctatcgtg acagaatccg cttctgaaat agctctgaac
    46741 ccttcatata cctctttgtc aatcgttacg cagtggtgga aaacatgatg ttcttcaaag
    46801 aattcagcta aggaagcata cggcacaaat tctcctgctt cgtttagaaa cagtgaacgc
    46861 gcatagccgc catatgcggc atcaacatgt aaatagaaat agatcccttc atcttttaac
    46921 ctttctctta attggacgat tttatctaca ctgtctactt gaccttcttc tgtcgttcct
    46981 acgacagcaa ccacacctag aattgggatt ttttgttcag ctaattcacg aattgttttt
    47041 tccaaagcat taatatccat tcgataatcc tcttgaactg ggatagcaac catctgatct
    47101 aagcctactc cgcaaatatc taacgccttc atccaagaat agtgttttgt ttgaggcacc
    47161 agccatttct ctaatctttg gatatttttt ccgctacggg aagaagctgc tttgacctca
    47221 tccatttctt cgtccgtaaa gctttcggtc atttccaaaa tttcttcaac agataaattc
    47281 aaaagttccc actcagacat ttttttcact tgttcaggca gcacttcctt gacagctaaa
    47341 ggaatcgatt tgatgcagcg ggcgtaccat aatccttcaa gattagcaat agaaccatct
    47401 gctgcaatgt gtccccatcc gtctgtcata ttgaataatg aagcaaaatc ctgtcctact
    47461 tctgcttcca tttgcgaagt tgccatagaa gattccaatg caacattatt tgggttccac
    47521 agcatcgcat agttgtaagc taataaagca ggcatcaatg tttcagcatt catttgtccc
    47581 cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc gtaatcgctg actgacttca
    47641 tccaagacct ctatcatatg gtcgcgggtg gctagataat ctggagtgag cttatctcct
    47701 tcgctaattg caggcatatc tgaaggaata taattttctc tccatccaag atgttcatct
    47761 accatcttgt tcaacaacat tttataaact ggtccatttt cagctttgtc gcctataaag
    47821 acggccttga tatccatatc tttcattttt cttccctcca tattttgttg gtcaatgtct
    47881 cgcagataca tgtgtttatc tgtaacacct cttcactata gcgccaaaaa tgagaaattt
    47941 ttttttataa catcaaatcc acgtagattt tta
//
LOCUS       DACCCR010000013        47724 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCCR010000013 DACCCR010000000
VERSION     DACCCR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.013"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(229..2121)
                     /locus_tag="IVK93_000734"
     CDS             complement(229..2121)
                     /locus_tag="IVK93_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6386046.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2118..3845)
                     /locus_tag="IVK93_000735"
     CDS             complement(2118..3845)
                     /locus_tag="IVK93_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6386047.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3861..4511)
                     /locus_tag="IVK93_000736"
     CDS             complement(3861..4511)
                     /locus_tag="IVK93_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6386048.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4715..5011
                     /locus_tag="IVK93_000737"
     CDS             4715..5011
                     /locus_tag="IVK93_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6386049.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5183..5803
                     /locus_tag="IVK93_000738"
     CDS             5183..5803
                     /locus_tag="IVK93_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP6386050.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5895..7061)
                     /locus_tag="IVK93_000739"
     CDS             complement(5895..7061)
                     /locus_tag="IVK93_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6386051.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7073..7426)
                     /locus_tag="IVK93_000740"
     CDS             complement(7073..7426)
                     /locus_tag="IVK93_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6386052.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7554..8576
                     /locus_tag="IVK93_000741"
     CDS             7554..8576
                     /locus_tag="IVK93_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP6386053.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9015..9449)
                     /locus_tag="IVK93_000742"
     CDS             complement(9015..9449)
                     /locus_tag="IVK93_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386054.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9559..11418)
                     /locus_tag="IVK93_000743"
     CDS             complement(9559..11418)
                     /locus_tag="IVK93_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6386055.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11536..12696)
                     /locus_tag="IVK93_000744"
     CDS             complement(11536..12696)
                     /locus_tag="IVK93_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP6386056.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13003..14427)
                     /gene="araA"
                     /locus_tag="IVK93_000745"
     CDS             complement(13003..14427)
                     /gene="araA"
                     /locus_tag="IVK93_000745"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP6386057.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14443..15141)
                     /locus_tag="IVK93_000746"
     CDS             complement(14443..15141)
                     /locus_tag="IVK93_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6386058.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15098..16759)
                     /locus_tag="IVK93_000747"
     CDS             complement(15098..16759)
                     /locus_tag="IVK93_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP6386059.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16977..18377
                     /locus_tag="IVK93_000748"
     CDS             16977..18377
                     /locus_tag="IVK93_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP6386060.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18629..19708)
                     /locus_tag="IVK93_000749"
     CDS             complement(18629..19708)
                     /locus_tag="IVK93_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6386061.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19794..20753)
                     /locus_tag="IVK93_000750"
     CDS             complement(19794..20753)
                     /locus_tag="IVK93_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP6386062.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20765..22288)
                     /locus_tag="IVK93_000751"
     CDS             complement(20765..22288)
                     /locus_tag="IVK93_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6386063.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22298..22861)
                     /locus_tag="IVK93_000752"
     CDS             complement(22298..22861)
                     /locus_tag="IVK93_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP6386064.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22867..23730)
                     /locus_tag="IVK93_000753"
     CDS             complement(22867..23730)
                     /locus_tag="IVK93_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6386065.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23744..24640)
                     /locus_tag="IVK93_000754"
     CDS             complement(23744..24640)
                     /locus_tag="IVK93_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6386066.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24815..26086)
                     /locus_tag="IVK93_000755"
     CDS             complement(24815..26086)
                     /locus_tag="IVK93_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6386067.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26076..27593)
                     /locus_tag="IVK93_000756"
     CDS             complement(26076..27593)
                     /locus_tag="IVK93_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6386068.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27791..28681
                     /locus_tag="IVK93_000757"
     CDS             27791..28681
                     /locus_tag="IVK93_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP6386069.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28754..29578)
                     /gene="nadE"
                     /locus_tag="IVK93_000758"
     CDS             complement(28754..29578)
                     /gene="nadE"
                     /locus_tag="IVK93_000758"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP6386070.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29590..31062)
                     /locus_tag="IVK93_000759"
     CDS             complement(29590..31062)
                     /locus_tag="IVK93_000759"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP6386071.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31699..32061)
                     /locus_tag="IVK93_000760"
     CDS             complement(31699..32061)
                     /locus_tag="IVK93_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6386072.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32284..33036
                     /locus_tag="IVK93_000761"
     CDS             32284..33036
                     /locus_tag="IVK93_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6386073.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33176..34099)
                     /locus_tag="IVK93_000762"
     CDS             complement(33176..34099)
                     /locus_tag="IVK93_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6386074.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34114..34386)
                     /locus_tag="IVK93_000763"
     CDS             complement(34114..34386)
                     /locus_tag="IVK93_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386075.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(34856..36481)
                     /gene="groL"
                     /locus_tag="IVK93_000764"
     CDS             complement(34856..36481)
                     /gene="groL"
                     /locus_tag="IVK93_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP6386076.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(36532..36816)
                     /locus_tag="IVK93_000765"
     CDS             complement(36532..36816)
                     /locus_tag="IVK93_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP6386077.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            37041..37703
                     /locus_tag="IVK93_000766"
     CDS             37041..37703
                     /locus_tag="IVK93_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6386078.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(37779..39071)
                     /locus_tag="IVK93_000767"
     CDS             complement(37779..39071)
                     /locus_tag="IVK93_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6386079.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            39241..39870
                     /locus_tag="IVK93_000768"
     CDS             39241..39870
                     /locus_tag="IVK93_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP6386080.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(39973..40782)
                     /locus_tag="IVK93_000769"
     CDS             complement(39973..40782)
                     /locus_tag="IVK93_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6386081.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(40837..41706)
                     /locus_tag="IVK93_000770"
     CDS             complement(40837..41706)
                     /locus_tag="IVK93_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386082.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(41707..43023)
                     /locus_tag="IVK93_000771"
     CDS             complement(41707..43023)
                     /locus_tag="IVK93_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386083.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(43020..44855)
                     /gene="walK"
                     /locus_tag="IVK93_000772"
     CDS             complement(43020..44855)
                     /gene="walK"
                     /locus_tag="IVK93_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP6386084.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(44860..45564)
                     /locus_tag="IVK93_000773"
     CDS             complement(44860..45564)
                     /locus_tag="IVK93_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6386085.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(45754..46614)
                     /locus_tag="IVK93_000774"
     CDS             complement(45754..46614)
                     /locus_tag="IVK93_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6386086.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(46601..47170)
                     /locus_tag="IVK93_000775"
     CDS             complement(46601..47170)
                     /locus_tag="IVK93_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP6386087.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            47551..47623
                     /locus_tag="IVK93_000776"
     tRNA            47551..47623
                     /locus_tag="IVK93_000776"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47584..47586,aa:Lys,seq:ctt)
BASE COUNT        13886 a        10031 c         8216 g        15591 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt aaaatcagcg
       61 gtttgacctg agtattagag atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt
      121 ttgagctaat accgaaggac agctgattga acaccgttta ttcggaaaaa gggactgtga
      181 cacgactttt gtcacagccc ctaaaaaaaa taataaattt gtactattct atgaagcttc
      241 ttcttcagaa aactgactgt tataaagatc tgcatagaat ccgtcttttt ccatcaatgt
      301 atcgtggttt cctgtttcta cgatcgaacc atgattcatc acaataatgt tgtctgcatc
      361 gcgtattgta gacaagcggt gagcaacaac gaaacttgtc cggttttcca ataggcggtt
      421 catcgctttt tgaatcagta tttctgttcg tgtatctaca ctagatgttg cttcatcaag
      481 gatcaggaca tctggatttg ctaagaatgc ccgcgcaatt gtgattagct gacgctgccc
      541 ttgcgaaata ttgcttgcat cctcgtttaa tacagtgtta tatccttctg gcaatttacg
      601 aacaaaatcg tctacgtgag cagctttagc agcctcaatg atttgttcgt ctgttgcatc
      661 gtcgttccca tatttgatat tgtcatagat acttcctgta aacagccaag tatcttgcag
      721 taccattgaa aactgtgcgc gcaattcatc acgtgaaaga tcacgggtat ctacaccatc
      781 gtaacgaata ctacctccag aaacatcgta gaaccgttca agaaggttga tcagtgtcgt
      841 cttccctgca ccagtcggac ctacaatagc gaccatctca ccaggtttga catccaagtt
      901 aaaatcagtc attagcaagt tatctggtgt gtagccgaat tgaacatttt caaaacgaac
      961 tttgtatggt gtatcttttt cttctggaag gccagatttt tcattggaca tctcttcttc
     1021 atccaacact tcaaataccc gttcggctga agcaactgtt gattggatag tgttcatcaa
     1081 acttgcaagt tgagtgattg gttgagagaa ctgtgttgta tattgcaaga acgcttgaac
     1141 atcccctaat gtcatgttcc catttgcaac ttttactccg cctagtacag cgacgaacac
     1201 atagcctaga tttttaacga aattcatcaa cggcatgatg attgccgaaa taaattgagc
     1261 tttccaacca gcggcataca gattctgatt ttctttttca aatacttcct ggtctttttc
     1321 agtatgattg aatgttttaa caatggtatg accaccatat gtttcttcta cttggttatt
     1381 caataaacct aaactttttt gctgatctgc gaaaaacact tgtgatttcg gcgctacgat
     1441 catcacgatg atcaagctta aagggatcat caataacgcg actaacgtca attgccagct
     1501 gattgaaagc atcatcacga tgaccccaat caatgtgact gcactagtta caaattgagt
     1561 caaattttgc tgcagcgtgc ttgcgatgtt atccatatca ttgattgctc gagacatgat
     1621 atccccattt gtatgtgtat cataatagga aatcggtaca cgattcattt tggcttccaa
     1681 atcatgacgt aattcataaa ctgttcgttg ggaaacacgt gtcataataa attgctgtaa
     1741 aaagttgaat acagcagaaa tcaaatacat cacgattact gtcgcaatga tctgcgcgat
     1801 tttatcaaag tctatcggaa attttgttac ttgttggcct tgttgcatct gttgagcgcc
     1861 agccattaca cctttgaata tttctgtggt tgctttcccc aagacctttg gtgtttggat
     1921 ttggaagatc acggctgcta ttgctagtac aaatacagcg atgatcgcaa ccatacgttt
     1981 agacatatag ccaaacaatc ggcgtaccgt tccccagaaa tttttaggct tttcaccttt
     2041 tacaccgaga tttctgccag gtccaccatg tcctggaccg cctgctggtc catgagaggg
     2101 acgtgtgttt gttttactca tgctaagtcc tcctctctta gttgtgaatg aacgatttct
     2161 tgataagttt cgtttgttgc taatagttct tcatgtgttc ctttgccaac cagttttcct
     2221 tcatctagca caaggatcat atctgcatcc attactgtac ttactcgttg agcaactaaa
     2281 acggtgatac tttctttcat attcgttttc aatgcttggc gaagattggc atccgttttg
     2341 aaatccaacg ctgagaagga atcatcaaac acatagatat ctgctgtttt gactaatgcc
     2401 cgtgcaattg ccagacgctg tctttgtcca ccagagaagt ttcctcctcc ttgttccaca
     2461 tggctatcta agccatcttc catctctgaa acaaaatctt tagcttgtgc gatttctaat
     2521 gctttccaaa tcatttcatc tgttgcatcc ggcttaccat actgcatatt gtctcggatc
     2581 gttccagaga acagaactgc tttttgagga acaaagccca ttaattctcg caaattatat
     2641 tgagtcatgt cactaatgtc cgttccattt atttggatcg aaccagattc gatgtcatat
     2701 aatcgaggga tcaaattgac taaggtcgtt ttacctgaac cagtcccccc tatgattgcg
     2761 acgatctcgc ccgacttggc ttggaaatca atatcttcca atgcaagttt ttctgctcca
     2821 tgatatcgat aattgacatg attgaaggcc aatgttgcgt tttttccagc aaaagaaact
     2881 gttttaggat tttcagggtc atggataccg attttttcat ccaacacttc attaatacgg
     2941 tcagctgaag cttgtgcacg aggcaccatg ataaaaatag cggaaagcat cataaagctg
     3001 atcaagatct gcatcgcata agtcataaat gcaatcagat taccgacttc caaagtcata
     3061 tctgctatgt agtgtccacc aaaccaagtg attgctacgt ttgtaccgct catgatcaat
     3121 gtcatcattg gcatcatcaa tgcgacgatc gtattaactt tgattgccgt atttgtataa
     3181 tctttattcg ctaaatcaaa acgattttct tcaaagcgtg tcttatcgaa tgctcgaatc
     3241 actctgaccc ctgtcaatcc ttctcgaaat accaggttta aacggtcagt tttcttttgc
     3301 atacttttga ataatggaac agcaaagtac atgattccgc cgatcaaaat aatcattact
     3361 gggatgacaa ctaagaagat ttttgtcaac tgatgatctt tttgataagc aagtatgctc
     3421 gcgccaatca acgtgattgg tgcattgatc atcatacgta aaaacatttg tgtaaccatt
     3481 tggatttgag tcacatcatt ggtcgttctt gtgatcaaag aggccgttcc atatttatca
     3541 aacgcatctt ttgtcagact ctctgatttt ttatagacat ccgtacgcaa ttgcttaccc
     3601 agtttctgtg attcacgagt agcaaaaaat gtatttgcga tagcagctaa gatactgatc
     3661 aaagaaaagc cgatcatcac aaaacctgta tgccaaatat aatcgacatc tccttgcgct
     3721 acccctttat caataatatt ggaggtaagt gtcggcaaat ataaatctgc cagtacttgg
     3781 atcaccatga aaactgcagc tgcaattgct gaagtcaagg agatccgttt tacgattttt
     3841 agcatctagt tcccttcttt ctatttatat actccgtttt tcagccaaga aagtttcaaa
     3901 ttgaattcac tgattgcttg ctctactgag taatcttcag atgttgaaaa taagcgatac
     3961 gaatgcgcaa ttgttgtaaa tacagtattc atcactaatt ggatcagtaa ttccaattca
     4021 tgatcatttt ttactttcag cttcgtccga tcgatcaatt caagcaactc ttttgcatct
     4081 tgtttgtgtt ctttatgtgg atcgtgaggc gttttttggc gactcgtatg aggagcgact
     4141 tttcttgagg cacggtaatc catgttcata aacaagttac gataaaaaga agcattctcg
     4201 ccttccaaaa tctcataaat cattttagaa aaataggatt cgaatccttt gaatagatca
     4261 ccatcggatt ctttcattag ttgtaacata tctttagagc tgtcacgtct cattgtctca
     4321 aagtaataaa aataaagatc ctctttatct tcaaaatact gatagaaact gcctctaggg
     4381 atatccgcta atttgacgat attagcaatc gaagcttctt ttaagggtac tctagaaaac
     4441 tcgattctcg cagcctcgat tagtcttttt tgtttttctt taggcaaatg gaaaaatgtc
     4501 tgtgtcggca tatggatcct cctttctttc aagatgacac attgtcatat gacgctgtgt
     4561 cattataaca cctcctacct gttttgcaag acatttgcat agtttttttc attatcatcc
     4621 gatacattaa aaaagtgata agaggaatcc ttccatttga attaatgaga gttgcccatt
     4681 aaaatagcca catactaaac aaaggatgag ataaatggcc caatcaatta gtgtccaaga
     4741 cttcaataaa ttgcctctcg atgctgacag catcgtttta gatacaagaa ataccttaga
     4801 ttacacaaaa aaccacttaa gccactcgct ttctatcccc gctgctatct tgccaagaca
     4861 attaaaaaat ctagatccaa agaaacttta ttatattcta agctatacag gcaggcgatc
     4921 agaagcatta gccgatcaac ttactgctaa tggttttcgt gctgtttata tcattggtgg
     4981 gatggaagct cttcagtcga gtgttgcttg aatattgctt gttagattca aaagaggctg
     5041 tgacaaaaaa tcgtgtcaca gcctctttta taccctgtct ttttttagtt atttattctt
     5101 caaccttatc aatatgactt gatagccttt tctttttctc atagttataa caatcatagc
     5161 aataaaagga gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac
     5221 tgcagaagaa tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa
     5281 tcatccagat gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga
     5341 tgaagaatta ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc
     5401 agaaagtcaa caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc
     5461 tgataaaatc gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc
     5521 ttggttcgat accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga
     5581 aggattagtg acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg
     5641 acaagacttt gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt
     5701 agatcagcta ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca
     5761 aaatgcatta gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca
     5821 tacaaaagag acagaggaaa aaatcttcaa agcagcattt gtaccttgga tattttccca
     5881 tctgtctctt tttgttattc tttctgcttt ttcaacggat tttcttcata aagagaagag
     5941 attgttgtgt accattcaaa aacatgagta atcactactt tagctgctgc ataaacagga
     6001 atccccaaga tcacaccgat gattccgaac aatttcccag aagtcagcag aactaataaa
     6061 atcgttactg gatggattgc taactgattc cctagtacta atggagagat caagcgtcct
     6121 tctattgtct gctctactgc aaaaacgatc agtactttga tgatcatgaa tggacctgct
     6181 acaatggcta aaaagatagc cggaatcatc gctaaaaatg atcctaagta agggatcagg
     6241 ttcaaaaaac ccgcaataat ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa
     6301 ccaatcataa acatgattgc cacagcaaaa gccacagtta gttgtcctct tatataagaa
     6361 gaaacttgac tattcatttc actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt
     6421 aaaaatttga caaaataagg tgctaattgt ttaccgtctt tcaatagata aaataagata
     6481 aacggcatcg tcagcagagc cactaaaata gtcgctaccg cgccgacaaa acttccaaga
     6541 ctctgaactg tactcttaga tatatcttgg atcatatcgc ctgccgaatt catgaactta
     6601 tcacctgctg tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg
     6661 gttaatttat tatcaatcgt atctacatat tgagggaagt tactgatgaa gctcactgtt
     6721 tgttcctgaa tcttagggat gatcactacg ctcccccatg cgattagtgc aacaaccaaa
     6781 acaaacagcc caatgatact gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact
     6841 accggattca tcaagtaata caagattcct gctaaaatga taggcagacc tacgattgct
     6901 aaaaattgcc agattggtgc aaacaaatat gaaacttttg taaataaaaa gacgatcagc
     6961 aatattaaca atacaacaag taaggctgtg actacttgat tatttaaaaa ccagcgccaa
     7021 aaccacgata ccggtctgtt cttcttattt tttccttcca taattcctcc tgttagttat
     7081 aaaaaatttg gcttcccaaa ccaatagcag gcagtctatg cggatgaaga tagattccat
     7141 ttgcaatttg tccttcttct ggacaagcat caaaaatcaa tgatacgtga gctactgtct
     7201 ctaaattccc attggccatc ggaccgacat aatcgatcgt atatacctga tcatcaaaag
     7261 caattgttcc gcctgctttc aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg
     7321 aatattttcg taaggcagct gttgctgttt tatcgaacaa aatcagcatg ggttcttcct
     7381 tgtttattgc ttctgtccca atttctgtaa tctttgcctg aatcattgca ctctctcctt
     7441 taattgcttt caaaaacagt ataacacatt ctcattcttt agccgtgtgg aataattatg
     7501 tgatagaata aaaacgaaaa ctatttcatt acaattagga ggaatcagct catatgatcg
     7561 aaaagataat tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc
     7621 ttgatacaga aaaagaagaa ttagccaacc tttcattagc tgttgaagaa aatagcccta
     7681 cctatgtagc aaaaagtaaa gctggcaatc tttacacagt tactagtgtg gatggattag
     7741 gtggtgccgg tgcgtacgat aaggattttc attttttgaa tgcggtaaca gagtctggcg
     7801 ctcctttgtg ttatgtagcg gttgatgaag cacgtcaatt actatacggg gcgaactatc
     7861 ataaaggtga agtaaatgtc taccatatat taggagatgg cgaactgaaa gcagcagatt
     7921 caatattcca tcaagaagcg acaggtcctc ataaaaacca agatcatgcg catgtccact
     7981 atacggatct gactcctgac caacgattag tcgtatgtga tcttggtaca gatcgtgtgt
     8041 atacttatga tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc actgcagagc
     8101 caggaacagg ccctcgccat cttgtctttc atccaaatgg acaattcgct tatttatttg
     8161 gtgaattaga cagcacagtc cgcgtgcttt cttataataa agaaacaggt gcttttgaag
     8221 aattacagaa aatatcgacc ttgccagaag aattcgacgg tgaaaatgga ggggcagcta
     8281 tccgcatttc aaatgatggc aagttccttt atgcttctaa ccgaggacat aactcgattg
     8341 ctgtattcgc agtttttgaa gatggttccc acattcaaaa caagcaaatc atctcaacag
     8401 aaggggattt tccccgcgat ttcgcactag atccaagcaa taattttgtc gtgtgtgcca
     8461 atcagaacac cgataatctg acattatatc gccgtcatcc tgaaacagga ttactggaat
     8521 tactgaaaaa agatgtctat gtaccagaat gtgtctgtgt ttattttgat cgttagtaag
     8581 aaaaggagaa tgaaatgaat cagcaagacg tattagacag actacgggaa gaactaaata
     8641 ttcctttctt tgatgggaaa atcgaagata aagagtatag cgaagaggaa tatcagaaac
     8701 tcaaagccga tttacagaac tactttgaac aatatgtaca gaatgtagaa aactaatcag
     8761 ctttccatac tatctgaaaa gaacatcagg acaagacttt tccgtctatc ctgatgttct
     8821 tttctacaaa taaaaaaccg ccgagatggt tagactcgac ggaaaaggag tatttactcg
     8881 attttgagta agtacaaggt tgttgttggt tataggtatg tcattatcat aaaggatttt
     8941 gatcactcta tcataaaggt aatattacat ttatattaga taactctagt ttttattgtt
     9001 atcaaagatt tcttttattt tttcaacaac aaattagtca ttttttggat ggcagctgtc
     9061 aattggtctg tcgcttggtc ttgttttgct tcagatgtat tgaatagatc ttcatctacc
     9121 cctgattcaa gatcgatact ttggatcatt tcttcacagc ctgctacata tgaaagataa
     9181 gctttttcaa aatttttatg aatgcccatt gcttttgctg gtggacgcaa agtactgact
     9241 ttcttaagca ttgcccgata tttttttgtt ccttcttcaa ataacaggat gatctcttca
     9301 agagtcgatt tgtccagttc acttgttttt ccttcatcaa ttgcgtttct gatcacttca
     9361 tatttcggat gcattccttc tccgattgct tctgtttcct tgacgatttg atcgatggac
     9421 tgcacataat agccaatatt cggtaacatg ttcgttttcc tactttctcc attgttgtta
     9481 ttatttattc tacttgtttt tatcctcaaa tgacaagtac ttgaaaaaat ttaatggttt
     9541 ctatacagct cgccattctt attcgtgctc aaagaaacca tctgctaaac tatattgttt
     9601 ccctgcgaca atgtcatatt ggatcaattg atattcgtcg taaatctctt gttgcgagcg
     9661 attgaattgc gcttctttat accactgtcc gttttgatag tacagttctc gttcaaacgc
     9721 cggaagttcc tgttctaatg ataaaagcag ctgataaaag ccatttgttg tctgattcgt
     9781 ctgttccatt agattcgctg caaaataaaa cggactggtg attgcatcgt gagtcgtttg
     9841 cttttctaat tctcctttac tgtcgaacat aagaaattgt gtttcatgca atgtttgttt
     9901 gtcatttttt gcttggatcg tatctgaata gatccctgga agatgatccc cccaaaaaac
     9961 aactaaagtc cttctagaaa gattcttcaa ttcttccgtg aactgcttca gtgccgtgct
    10021 gctataggaa atatcttgca gataattctc taagtcaagt gtcccacttc catctgaagt
    10081 ttttgcagga tagtctagat tatcatactt tccatcgtag ggcatatgtg tctgcatcgt
    10141 gactaaatga atgaactgag gtgttttttc ttccttgaga agtgtcatta cttctttgta
    10201 agctgactca tcagaaatat atggattatt ctctatcgta tccgtatagg tcatcgtacg
    10261 ctcgctgatg aactggtcaa atcctaatgt ctgataaaca tcctctcttt tatacatcga
    10321 agtattgtat ggatggatcg ctgtcgtctg gtatgattgt gcgtttaacg ctgaaacgat
    10381 agaaggaagc tgatccattt ttggtacaag catcgtgtaa ggggtggtga gttgcgcatt
    10441 aaataacgcc atagaaaaac cagtcaatgc ttcaaattcg atatttgctg ttccaccgcc
    10501 atagttttga gaaagcatat tgccactata tgtttgatca gctacttcat aataatctgc
    10561 caatggctca ccagatactt ctacgccgtt taatcgacta ggatctgaga aactttcact
    10621 catcacgaac acgatgttcg gcgactcttc ctctacagtt ttattttttt catcggctag
    10681 cttctggtat ttttcagtga tttctttgat cttttctttc gaatatcctt caggttcatc
    10741 cattggttct accttcagat tgtacaaaaa cccaccgata aaaccagtgt tatagtagtt
    10801 cattttctgg ctgtaaggta tccatagtgc cgttttatta tatgcttttc gcaacaaatt
    10861 atcgggattg ttgaaattac tgatataccc tagcagtcca attgccacaa gcagtgtcgt
    10921 taatcggatc aattggaatc gccttccttt tttcaggctt cgaaacatgt accagcaaaa
    10981 caatcctatc acggttcctg cggcagctaa gatcactgta aacagcatag tgccggtcat
    11041 atctttcagc aatccgattt cagtgatcat cttcaaatcg tctggataaa tcggttcttg
    11101 ccgataactc attttcatat aatttgcaaa acctaataca ccgatggcta cgacatacgt
    11161 aagggaacct aaagggacag atcctgctag tgcaatcaga aaaataagaa taatcagcaa
    11221 gacgagacag gctaagaaga atttttccgt atgccaagaa aaagcaaatt tcaacgcaag
    11281 gtctacggat agatcatttt gacaccactg caaaaataaa ttactaaaaa caacaagaaa
    11341 aaagatactg atgattgctg ctgttccttt taaaatcttt ttctgtttgg tcgaaagtct
    11401 gcttatatat cttttcaaat gcttgctcct tcatttctat caataaaaga atgttgcttc
    11461 tcttatttta gttgtacgaa attcgaaagt aaagaacgga aagaagaatt aatgaaatcc
    11521 aaatattctc tttctttatg gcctttctgg tttctttaat aagcggacct ttcgtactcc
    11581 agaaattttt tctagctgtt gtttcacttg attgagctgt tcaaaatcag tactgtctat
    11641 gtcaatcaat atgtagacat gctcattctt atggttacgc gatatatctg cgatcgctag
    11701 ctcgttctca ttcagcagag aaaatatctc tgctaatata ttggttttct tttgataaaa
    11761 tatagtgaat cgaaacggcg cttggaaaaa cattcgtgcg gatggataat tgaccgcttc
    11821 tctaaccgta ccgaacaaaa gataatcttt taaggcttca actgccaaac gtccgctgtc
    11881 tgccaaagct tcctgagtcg atccccctag atgaggcaac ataagtatac ggtcattttc
    11941 taagaactct tcttctggaa aatcagtcaa gtatttagcc agatgttgtt tttccaatgc
    12001 ttgcataaca gcatatttat caacaatttc acttcttcct acattgataa gtacagcatc
    12061 ttttttcatt ttttctatat tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg
    12121 cagcatgact acgatgtaat ccgaggtaga aagaactgtt tctaaatctg cttgccgaac
    12181 ataatccaac tgggcatcgc ggatagaata acccaagaca tccatcccta agctatagca
    12241 acttagcgct acttttgtgc ctatcgctcc aagaccaaga agaccgatgg ttttgccttg
    12301 caattctcgt cccacataag cacttctttt gttttctgcc tgttctaaaa tattaggacc
    12361 tgttagtgtt tgtaccatac gacttgcttc gattatcgga cgggaagata agagcaaaca
    12421 gcacaaaaca agctctttta ctgcattagc atttacacct ggtgtattca tgacgatcgt
    12481 cccgttttca gaagcttttt caacgttgat cgtgttcacc ccaacaccag ctcttgaaat
    12541 tgctagcaaa tctggtgtaa gccattcatc tttcactttc gtactacgaa taactattcc
    12601 ttcggctggc aaatcatcta ttattttgaa atattcccct tgtaactttt ctatctcttg
    12661 ttctggaata gcatcaatca aatgaacgtg atacataaaa ccccctcctt actaacaagt
    12721 atctcgaaat ttctgcaatt agacaactgt ttaccatttg acttcctaga agcaaaaaga
    12781 gcagttagag cagagtaggc tgagtgataa aaataaggct atgacaagtt actgccataa
    12841 gccttatttt cgagagcaaa atattgaggg tgagatatct tgataaatgg tgttccgtta
    12901 gctgacattg ataataagac taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc
    12961 ttcttatttc ttaaaactaa gtggcttttt cacaacctct ttttatctaa taaccactgt
    13021 ttccaactca actaattttg cccacgtttc aatttgatct gttgttagat ttagcgagac
    13081 aacggtatga tgaccgccac ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg
    13141 tttagttttc cataacacac gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa
    13201 ggcttctact tcattgatca gcagtttata atgcgtaccg aaatctgcca tcgatacgac
    13261 taccccttct cctgctttcc catcaaatac aagacgcgcc ggatcttctc tatctcccat
    13321 tgataaagga gaaacaacga ttcttggttt cgtcgcagca agcgtcggat ctacttccat
    13381 catatgggat tccaagattg cttcttggcc actagctagc tcgtaagtat aatcttccat
    13441 gaatcctgta tctttccctc gagccatgat tttcagcaac cgatcaatcg ctgcagtttt
    13501 ccagtcgcct tccccagcaa aaccgtatcc ttctgccatc aaacgttgta cagctagtcc
    13561 aggtagttgt ttcattccat acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc
    13621 ttcttctaag aaacggcgaa taccgatttc ctgctgagct tgtactttta catgttcctc
    13681 ccatgtcttt tcttcataat caccatagtc gaaggtatac aattccttgt actcttcaaa
    13741 taattgatcg acttcttcgt ctgatacgcg atcgattact tgtacaagat ctccgatacc
    13801 aaaataatca acagtccaac caaattgaat ctgtgcttcc actttgtctc cttctgtgac
    13861 acccacgttt ctcatgttgt caccaaaacg agccactttt attccaaaac tttcattata
    13921 ggctactgca acatccatcc aatcagcaat ctctttttgg atctcaggtc gtttccagta
    13981 gccaacgacg attttatttt gtttatttaa tcgagcattg atgaacccat attcgcggtc
    14041 accatgagcg gattgattta aattcatgaa atccatatcg atcgttttcc aaggaatgct
    14101 ttcgttatat tgcgtagcta aatgaagcaa tggtttttgt agaagctttg tcccgcggat
    14161 ccacattttt gctggtgaga aagtatgcat ccaagtgatc acacctgcta cttcttctcg
    14221 ataattgacc tctttcatga tttttgtgat cgtatcagca gtaactgcta attcttgaag
    14281 gcggattgga taaggcaatt ggccattttc attcagttca tcgaccattt cttgtgcatg
    14341 tttctttact tctcgcagtg cttcttcccc atataaatgc tgtgatccta caacaaacca
    14401 aaattctttt tctccaatat tcaacataat tagccactcc ttttatttgt tttgtccgta
    14461 atacgcattt tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt gaggcagctc
    14521 ttctgaaaat gaattgagct gtctggtaaa taaattcatc ttgcatactt catctagtac
    14581 gactgcgttc attaccgcac tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg
    14641 taaaagtacc cctggaattg ctaagggatc aattttgcgt tctttgaatg tttctatgat
    14701 aacttttcct gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc
    14761 acaaggtacc gatccataaa aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa
    14821 tcctgcttgt gcccagatcg tcgcccatgt cgagtgagta tggacgattc ctccgatttg
    14881 aggaaatgaa cgatataaaa cagcatgagt aggcgtatcc gaagagggat tgagtttacc
    14941 ttcaatgata tgattgtcca agtccacaac gaccatgtcg tctgctgtca tctcttcata
    15001 actgactccg ctaggtttga taacgaaata tcgtgtttca ggatcaaaag cgctcacatt
    15061 tccccatgta tatttgacga gtccgtgttt aggcaagtca agatttgctt ggtatacttc
    15121 ttctttcagt tgttccagca ttttctatca ctcctttttc ttgcaattga tgaatcgctg
    15181 ttttttcaat gtcgatcccc tcacggtaac gtctcataaa tatctgatag cctgctattt
    15241 cttctgctga aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga
    15301 agttttctaa agacttccca tgtcgatcca tcatataggc tgctaataac gcgattcccc
    15361 acgctccacc ttctccggct gtgtccatca ctgtaactgg tgcctccata gcagatgcta
    15421 gaatccgctg tccaacttct ggtgttttaa aaatcccccc atgccccact aaacgatcaa
    15481 tttggacatt ctcagatttc aatatatcca ttccgattct cattgcacca aaagcactgg
    15541 ctaaatggat ccgcatgaaa ttcgctaaat caaaacggct acctggtgta cgtacaaata
    15601 atggccttcc ttcatttact cctgtaatgt tttcaccgga aaaataacca taagaaagta
    15661 atcccccgca atccgtatcg ccttctagcg ctttgttgaa taataccgtg aataatttat
    15721 ctctgcttat ctccataccc aaagaattgg taaattcttc aaaaatactg acccaagcat
    15781 tgatatccga agagcagtta ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag
    15841 tgaccatgtc gatttccgga tggacatttt ctagttcttt ttctaatacg atcatagcga
    15901 aggcggaagt accagccgaa atgttccctg tccgtttggc tacactgttc gtagcgacca
    15961 taccggttcc tgcatcccct tctggcggac aaaccgggat tcctggtctc aaatttcccg
    16021 tagggtcgat cagataagcc cctgattcgc ttaacttgcc agcttcttct cctgctacta
    16081 gaactttagg caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg attaactgac
    16141 caaaaatatt tttcatttct tgatggtagg ttttcgttga aggatcgata ggaaacatgc
    16201 ctgatgcatc tcctacgcct agtactttct caccagtaag ctgccaatgg acataacctg
    16261 caagcgttgt caaaaaatta atttctttca catgttcttc tttattcaaa atagattgat
    16321 acaaatgagc gatactccat ctctgcggaa tattgtattg aaatttttcc gttaatattc
    16381 gttctgcttc ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt aactgttcct
    16441 tgctatcaaa tgctagataa ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg
    16501 taagattctc gccatagatt tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca
    16561 gtcctttcca aatatcttct aatgaatagg tccagatacc attttctagt ttgttttccc
    16621 actcataact accagcggca ataggagtaa aagatgaatc aatcaataca gctttgatcc
    16681 tagttgatcc aaactcaatc cctaaagatg tacgaccatc tatgacatct aacatacttt
    16741 gcgcatttac ttccttcata atgatttaac tccctccttc tcgtcaaacc tgatcaaacc
    16801 gtctttttag tagcgcttac aatgattatt gtatactttt gtacgtacat gtcaagcggt
    16861 tatttacaag aatacaataa aaaacacttg ttttgtacgt acataagtta taaaatgaaa
    16921 ttgcttacaa aatctgatca accgccttct cgaaattcta gaaggagaga atacttatga
    16981 aaaaagaaaa aaaagtttcc tcatcgttta tttatttttt tggcgcattt ggcggcattc
    17041 tatttggata tgatattggt gttatgaccg gcgccttacc ttttttacag catgattggg
    17101 gtttggcagg taaagcttct ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga
    17161 tccttggcgg ctctttatct ggtcttcttt ctgataagct tggacgacga aaaatgattc
    17221 ttctttctgc acttattttt atggctggtt ctgtcctttc tgcatctgcc cctcacaatg
    17281 gttcttattt tctgattgca gcaaggattc ttctaggttt agctgttggc gctgcatctg
    17341 ccctagttcc tgcttatatg tctgaaatgg ctcctgctag actaagggga agattgtcag
    17401 ggatcaatca agtgatgatt gcttctggca tgctcctttc ctatgttgca gattatttgc
    17461 taaaagtgtt gcctgaaacg atggcttggc gcgtgatgtt agggctcgct gctgtccccg
    17521 cgctgatttt atttttcggt gttttagctc taccagagtc accgcgtttt cttatgcaat
    17581 ctggccgctt ggaagaggca aaaagagtat tgaactatat tcggacacca aaagaagctg
    17641 aacaggaatt cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc ggaacttctt
    17701 ggcatacttt gtttttagaa aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag
    17761 tattccagca atttcaagga gctaatgcta ttttttacta tattccgctg atcgtagaaa
    17821 aagcgactgg gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa ggaatcattt
    17881 tagttgctgg ttctttgctc tttttggtta ttgcagacaa gttcaatcgg cgaactttat
    17941 tgaaaatagg cggtagcgtg atgggacttt cattcatcct accagctgtt ttaggaactg
    18001 tactggatgc tcatacaaat tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat
    18061 tttattcttg cacatgggct ccactgacat gggtaatcat aggcgaaata ttcccactag
    18121 ctgtccgcgg gcgagcatct ggcctagcct cttcttttaa ttggattggt tctttcctag
    18181 ttggcttgct ttttcctgtg atgactgctt ctatgtctca agaaatcgtt ttcggcattt
    18241 ttgggatcat ctgcttttta ggggttttat ttattcaaga gatcgtacca gaaactcgtg
    18301 ggaaatcttt agaggaaatc gaacaaagtg catctaaaaa aacttatcct aaacgtattt
    18361 ctatccatca ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga
    18421 accgcttagt ttccagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa
    18481 tattgtgact tgtgaccagt tggtaggaga acaccgttta gtggagaggt ggtgaaagaa
    18541 acgttcagcc ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt aaccgtagga
    18601 gcaataagag gcggtaaaaa cccgtctctt atttttcgtt ttctttcact gtttcccgtt
    18661 caactagtac aggttcatag agaatatttg gctgtgcctg tcctttttca atcgttgtga
    18721 cgatccaccg tgcggcatct tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt
    18781 ttttattccc taaatggctt aaagaagaat cgtctgttcc tacgatcgat aggtcatctg
    18841 gaatatgata gccattttct gtcaacttat ctagcattaa actagctatt tgatcgttat
    18901 agcaaatgat gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga
    18961 ttgtttctcg tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat
    19021 actgttcaca cgcgctgata aaacctttca tcctgtattt tccttgaaga tcgtcgattt
    19081 ttgtgatgaa caacagacgc tgatgtcctt tttttatcaa atattctgct gccataaaac
    19141 cgactttcac atcatctaaa caaatataag aggcatttaa ctcttcataa gcagcattga
    19201 tcatcaccat cggaatgcct tgttctctca aagcaacata aagtgctaag tttgggttat
    19261 actgattact tttcgtaggc tcaacgatca aaccatctac cccataagca atcattttct
    19321 tcaaacactc tttctccaat tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc
    19381 cagattctcg taattcattt tcgattcctc ggataatcga cggaaaaata taatcagaga
    19441 gatatgtggt gatgacaccg atcgtttttg ttctgttcaa tggtgttttt tctttcttat
    19501 agctgtcatc tacatatgtg cccgaccctt tttctttacg cagatagccc tcatttacca
    19561 acacagcgat tgcttgccga atcgtatgtc tgctgacgtc atatatttct tgcagcttga
    19621 attctgaggg aatggttgta tttacttcgt attcctcacg aaggatcttt tgttttatct
    19681 cttctgcaat atcttgatat tttgccattt gccttgcccc tttttatttg tacgtacata
    19741 tatcataacg agtctgcaag gtttattcaa gcaaaaatca aggtttcttg tgctcataag
    19801 gcatacggaa caggttcacc aaattctgga aatccttgct cattccacgt aaagcattgc
    19861 gcgttcgcgt gccgattagg attgtctaac ggatcccctt tttggttctt ctctggtcga
    19921 gcatgataga tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca
    19981 ggaccaaatt gcttattttt ttcagaagaa acgaatactg gctccgataa tttatgccat
    20041 gattctttta ataaaagatt cttgtgctca tcagcccaca gcaggcccat ggcatagttt
    20101 tcatctgttg cactaccaga atacgtaatg aatattttcc cattgcgagt aaggactgct
    20161 ggaccttcat ttactgaaaa accccgtttt tcccattcat actcaggtat agaaaggagg
    20221 atctgcttcc cctttaacgt ccaaggatta tccatttcgg atagataaag attagaattc
    20281 ccagtaattt caggatcttg ttgggcccac acataatata gctttccttg agaagagaaa
    20341 gttgttccgt ctaaactgaa agtttccctt ttcgttttta tttgtccctt ttctatccaa
    20401 tctgtattca ttggatctat atgctgattt tcaagtacga acatgcggtg atgatgagat
    20461 tcatcacgta tagttttatg attgcttgca gcaaagtaaa tataccattt tccattgata
    20521 taatggactt caggtgccca gatcaattca ctcatcggtc cttcttcatg agcctgccat
    20581 acaatcaggc ttgcagcatg ttctaattca ttcagtgact ttgcccttct taattcaatc
    20641 acttggtagc ctggaaccga tcctgtaaaa taataataac catctgtatg tttatatacc
    20701 caaggatctg cccgttgccg aacgatagga ttattataaa gtgtcttcat cataacaaat
    20761 ttcctcacat tttctttatt ctaattacat tccaagagta tttttctatc cctacattta
    20821 attcgtcatg tttttctatt gaaaaatagt ttatttcagg ttttattttt tctggttctt
    20881 cacgtgtatt tttgtccaac agatcgctac tggttaatgt gtagtgctct ccttgatcta
    20941 gcaaattgaa atcatgttca atcgttatct tttgttgttc atttgtgcga ttgatcaaga
    21001 aaatagtcag ctgattgccg ttatctacga tcacactgtc cacataagga acatttttta
    21061 tcttttcaca atcataaaca gggcaaatga tttgttgttt caatacttga cctttagcat
    21121 atttcgataa gtgttgcatc acataataag ttggattcct ccatgcttgt cctttagcat
    21181 ctgtcagtat taaaggtatt gtgtttacta acaatgattg acaactaatc tttacgcgat
    21241 ctgcgtgtcg taaaatagcc agccctaatg aaccaaataa taatgtatct tccatagtaa
    21301 agtaacacca atcgatggga ctaccgactt caaaatccct atgtcttttt tccggttggg
    21361 catgtacatt ccattcatca aacgctagat acattgtttt ctcacttctc aaatatcctt
    21421 ttacataatc acaagtagca acgatttctt caatttgacg atcgaatcga taagcttttg
    21481 caaggtatgt cgctgtatca tcacgctcat ttttaggagg tttagtcaag tcttcttcat
    21541 catacttatc aatatagtta tgcaaagcta aataatctac attctcataa gcttcttcta
    21601 atgcgatacg atcccattca gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga
    21661 ttgtttcaat tgaatcatcc actaacttca tgacttttga ggcttcattt gctaacctac
    21721 catattcata agcactctta gcccccactt gccatggacc gtctagctca ttccctaaac
    21781 accataattt aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga
    21841 aggttccttt tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg
    21901 ttcctaaatt caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt
    21961 cgtggagtcc aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc
    22021 gttgttcttt cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag
    22081 gatagcgaat gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc
    22141 cgtcaccatc agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca
    22201 tatgctcaac aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc
    22261 tgatttttac tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca
    22321 agataataga tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa
    22381 gaaatatgag cagtacaaat cctaataaca atacgcaaca aagaatattt ctcaaaaaat
    22441 caaaaaatac tttccatgca ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt
    22501 cagaaatcac atatatccag gataaaagaa acatggaata aataagtaaa actacatatc
    22561 cactaattga aagtacgatt gtctgttctt ctaataattg attaacaaga aaataaataa
    22621 tgatcgttaa cagactaagg aacaagttct taccaaaatg ttgctttatt ccttgaaaga
    22681 aaaaataaat gatatttcct gatccctcac ctttccattg cgccattaca taaaaaacag
    22741 cacatgtagc aggcacgata gtaataacag gcaaacacac gattacccag agaataccta
    22801 ataagatgta atcagaaacc acaatcaact gtccataaaa tttagtctca actattttca
    22861 tcttcctcac tctttcattc cagacatagc tgttccttga acaaaatatt tctgtgcaaa
    22921 aatataaacc actagtaatg gcagcattga aataaccgtt ccagccatta caggtccata
    22981 aaaagaaatg ttctgccctg taaataaagc taaccctgct ggtaaagttc tcatttttga
    23041 actacttgtc aatatcaatg gataaagcaa gtcattccaa ctattcatca gcgtgaaaat
    23101 ccctaatgat aaaagagtag gtttgctcaa tggcaacatg attttcaaaa aaatattaaa
    23161 ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag taataaaaaa
    23221 ggaacgcatc atgaagatac caaatgcagt tgtcatacga ggaatgatca aacctgcata
    23281 agtatcaagc atgccaagca aattgacttc aataaataga ggaatcatga agacttggaa
    23341 tgggatcatc attgtcagca aaacgaaata gaacaaaact gattttcctt tgaaacgcat
    23401 acgagcaaat gcataccccg caagagaatc aaagaataca gaagtgatca ctgtccctcc
    23461 agcaaaaatc actgtatttt tgatgtaatc taataaaggg atcgttgtcc aaaccttaat
    23521 atagttatct agggtatagc tatctgcaaa tattctaggc ggaaatgaga taatgtcttt
    23581 ttcgtgttta aaagaagaaa taatcagcca caaaaaagga aacgcaataa ccaacaatat
    23641 aactagcata ctaatcgttc gaatgatttt tttaccacta actgcttttc gactattcac
    23701 agtaactgta atcgagcttt ctggtgccat acaggtttcc ctcctatttc atttgttttt
    23761 ctttccgtga catgtacaca ttagtggaga tagctatagt tgcaacaatg ataaacaaaa
    23821 ccgaagacag tgctgatgca tagcccaatt cataaggtgc tgtaaatcct cgactgtaaa
    23881 tatattgaac agctgtttct gtcttaaatt gtgggccacc agcggttgca acatatactt
    23941 ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct aaggagggaa
    24001 cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca tccatttgag
    24061 cagattcata caaagaggga gagattccct gaatagcagt caagagaatc gtcatagtaa
    24121 caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg gttggatcgc
    24181 gaaaaaattg agggatcggc aagccgactt gtgaaaacaa gtaaggaatc gcccctacat
    24241 tcggatcaag caacatagaa aaaataatcg caattgacgt caatgaacaa ataactggaa
    24301 tgtaaaatgt cgaacgacaa aaacgattga atctggtatt tttagataat gctactgctg
    24361 ctaataaacc aacgataatt tgtgttggcg tttcgagtac ggtgaaatat aaagaatgaa
    24421 tcaacgaatt aactgctcgt tcatcactaa atatacgtgt gaaattttca aaacttacaa
    24481 aatttcttga tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat gttcctaaaa
    24541 gcggtatgac agtaaataat aataaaacta aaaaagatgg agttaaaaac aaatacgcag
    24601 cacgatcagg acgattccaa tatttggtga atccgttcat atagtccacc tcatctttca
    24661 ttttgataaa atatacaatc ttggtttaaa cgaatctgaa taagatatcc gaacaatcgc
    24721 tgtgattttt gcaataacac ctatctgcaa atactaggtc gatcattcag atatctgtat
    24781 cttgccaaca gtctatcctg tataaagttc tgtactattt ttgcagtaat tgattgatgg
    24841 catcatctgc atcgttcatg agtgtctcag gatcttctcc tgctaataat ttttcaatca
    24901 gtggattgat gatatcgtta ttgatcgcag agatttgggt aaaacctggt aaatatggct
    24961 ctgcatagtc catttgtttc gataattcag atacaatagg atcatttttt acttcttcat
    25021 cttttgctac tgaatccaag taggcaggaa aaccattttc gatactccat tttttaccaa
    25081 tttcagttgt attccaatat ttcaaaaact catagacagc ttctttttta ttattatctg
    25141 tacttgccgg aatggcatat cctacgccat ctaaaatagc tgtttttttg tctgaatcac
    25201 tcccttgtgg taaggtagtc acattataat caatctcgtt tgctttcaat ccgttattta
    25261 accatggccc attgatttca atggctagtt gaccagcatt cattaaattg tccatttcgg
    25321 cgccagaaat cgattctgga ccagtcttgt ctacatggat cattttttgg atcgctttta
    25381 acgttttaag gttctcagga gacgcaaatt cagacttagt ataatcatcg ttcactaatt
    25441 taccgccatt tgctaacatc cagttatata acggtgcagt tccatcctta ttgaagacaa
    25501 aacctgatac atttttacta ctatcagtta attttggtgc catttctgtc aattcttccc
    25561 aagtagtagg tggttgttca gtatctaatc cagcttgttt aaacaaatca ttattccaat
    25621 acatatacat gccttgtacc tgcatgggga caaaatactg tttaccttct acttcaccta
    25681 attcaatagc cgtttcttta aaatcagatt tatctactcc atcatattcc cagaaatcgt
    25741 ctaatggttg taccgctccg ttttctacat actgtgcaaa atctggataa tttaatgcaa
    25801 taaagtcagg tgctttttta gaagaaatcg ctgtaggaag tttttcattc aaattggccc
    25861 aagtcatgat atccatttca attttgatgt ttttatcatt tgttttatta aattcattaa
    25921 cgatttcttg caatatttca ccatcagatg cagtaaaccc attccaaaaa gtcaaagtca
    25981 cttctttatc agaatcagat tctgctgaac ttccgccacc gcaagctcct aaaatacctg
    26041 ttgcagctac caacaaagag ccaattaacc attttttacc tttcattttt tgttcctcct
    26101 tcatctgata aacgacatct gaccacattc caagatagcg gtttcaataa acaattgatt
    26161 ttcccatctt cagcttttac atttgttgaa tgacttggtt ttacttgttc acttttagga
    26221 agattagttg cttttgtatc aaatccactc atttcagaaa aatcaatcac tccctctaat
    26281 gataattcag acaagtgaac agttagattc atttcttctt ctccacggtt aactgcaaat
    26341 atgacaagtt ctttgtcatt tattacggct atactttcta aatacggcac ttcttcaaaa
    26401 tcttccgtcg catagtttgc tgaacgaatt tgtgggctta acacctctcc ttgtccatac
    26461 acagcagctt gcataaatgg ataatagatc gtctgtctcc aagcttctcc gtttgtttct
    26521 gtcctgattg gtgcaataac gtttaccaat tgagcaagac aagcaatctt tactctatct
    26581 gcatgtttca ataaagtgat taatagacag cctaatagta atgcatcttc aaaattatag
    26641 ttatcttcta gtaaaggagg agcaatctgc caaggcgctg cttgctcatc ttgttccgct
    26701 gaatgatacc aaatattcca ttcatcaaag gacaggttga tctgcttctt actatgtttc
    26761 ttcgccttga ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc catgtctaag
    26821 gaacgtgcaa gataatgaga aagatcattt ttttggttgc cataatattg atgcagagac
    26881 aaataatcta tttgatcgta cgcttgatca agaactgtta actcccaatc accaaatgta
    26941 ggcatttgcc gattcgaact tccgcataaa accacctcaa ttgaatcatc tacgagtttc
    27001 atcgccttag ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt atgtccaatc
    27061 tgccaaggac cgtccatttc attccctaaa caccatgtct taattccaaa agggctctct
    27121 tgaccattcg aacgccttaa atcactccaa taagttcctt taggaaaatt acaatattcg
    27181 actaaattac gagctgcatc tactccacga gtacccagat tgacagccat attgatttcg
    27241 gcccccacat gctgacacca gttataaaat tcatgaatcc caacttcatt cgtttcaatt
    27301 gtccgccaag ctaaatccaa ccttctaggt ctatttgcta tgggcccaat actgtcttcc
    27361 caacgaaatc ctgacacaaa atttccacca ggatatctaa tcatgggaat gttcaattct
    27421 tttaccaagt gaatgacgtc ttggcgaaat ccacttttat tagctgatgg atgatctggc
    27481 tgataaatcc cttcatatac tgctcgtccc atatgttcaa taaatgaact gtacaaccgc
    27541 ggatcaactt tgcctacacg cttgacacta ctaatcaaca ttgtggcttc cataactgac
    27601 ctcctttaat ttatacatga ataatacacc tcgttgaaag cgttgtcaat aatataaatg
    27661 ttaaacttac ttatttttta tcgattttaa ttaaccaaaa tattaataat gattaaaaca
    27721 aataatttaa tataactttt attaacaaat atgttaatat ataagaaaaa ccttttaaga
    27781 ggtgatatat atgcaactac aaataaacga agaatcctta ccggtatatg aagcattagc
    27841 tagtaaaaca aggatcaaga tcatccagct actttccaaa aaaaagatga atgtcaaaga
    27901 tttggcaaaa gaacttggag tgagcagtgc tatcacgacg atgcacgtga aaaagctaga
    27961 ggaagcaaac atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga gccttcgtgt
    28021 cgataaaatc gatattagtt ttccagaaaa aatattcaat gcttttgata cgaaagaaac
    28081 atcgatacct attggtcact atacaaatta tgcaattgaa ccaacatgcg gtttagctac
    28141 gatccatgat tttatcggga aagtggatga accacgctat tttatggatc ctcgaaggat
    28201 ggatgcccgc attctctggt ttaccagcgg atttgtcgag tatcaggcac ctaatttttt
    28261 aaatacagag gatacattgg agatgttgga agtttctgtg gaaatttctt ctgaatttcc
    28321 tttttcaaat gacaattggc cttcagatat cactttcagc ctaaatggtg tggagctagg
    28381 aacatggacg agccctggag attttgctga tatccgcggt aaatatacgc ctgattggta
    28441 tccagataat cttaatcaat acggcttgct gaagacgatc cgaattacca aacacttgac
    28501 gaatatgaat ggagaaccct tgtcaaacat cacgatcaac gacatcccta aagagcaaga
    28561 cacttggcat ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg gctgtacttt
    28621 atttggtaaa ggattcggta attacgatca ggatatcaaa ttaaaagttt actacagctg
    28681 atatttttct tttctatttt ataaaacaaa aacagccaat ccctatgaag ggacggctgt
    28741 ttttgcttta ttgttatttc cagaaatcat ccaaaacgga aattggcaaa tgtcgtttat
    28801 gttgtgtctt gttccaccat gcttcgattt tttcttgtgc ttgagcggga acttcttttc
    28861 cttctagata atcatcaatt tcattatatg tgacacctag tgcgacttca tctgcaatca
    28921 ttggtttccc atcttcaaga tctgcagtcg gaatttttgt gtataatttt tccggtgcat
    28981 tcaatgcttg aagcaattgt ttcccttgtc gtttgtccaa acggaataat ggcaaaatgt
    29041 cggctccacc atcaccaaat ttcgtgaaga atccagtaat gttttctgca gcatgatctg
    29101 tccctaatac ggctcctgct ctttctccag ctacggcata ttgcgtaatc atacgttgac
    29161 gtgctttgat atttcctttg ttaaaatcgc tgattcccac acctgcttcg cttaatactt
    29221 gtacttgtgc atcaacagct ggtttgatat ttactcgcag actaacatcg ggctggataa
    29281 agtcaagagc agcttttgca tcttcttcat ctgcttgttc accataagga agacgaacag
    29341 cgataaattg atagttttca tcttttgttt cttcacgcat ttcttccatc gttagttgcg
    29401 ccaaacggcc tgctaaagta gaatcttggc cgccgctgat acctagtaca aaagttttaa
    29461 gaaacggata ttttttcaaa tatgctttca taaagtcaat acttttacgt atttcttctt
    29521 ttggatcaat tgttggtttg acacctaatt cattgataat ttctgtctgc aggctcattt
    29581 attctcctcc tatgcttctg tatgaagttc tgctacgctt tttttcattc gttccatgat
    29641 agccatttta tggttccagc attcagtaga cagatcgact gggtattttt gcggattcaa
    29701 gtctcgttta tactcttccc atagtgaatc tagattttct tttgtatatt ctttgatttc
    29761 gtttagtgca ggtaattcgt aaactctttt tccttcgata aagatatctt gtaaaactgg
    29821 gcgagctgta aagtctcgaa cagttttatt gataaacgta tgcactggat ggaacatgaa
    29881 gatagcttct tcttctcgag ggtcttcatc ccataatgtc acgtagtctc cttcagattt
    29941 cccatcaaaa tttcgagtga tgcgccacac ttgttttttc cctggagtag ttactttttc
    30001 tgcattgctt gataacttga tcgtatccat catttttcct tcatcatctt cgatcgatac
    30061 tagcttgaat actgcaccaa gggctggctg atcataggct gtgatcaatt ttgtcccaac
    30121 accccataca tcaattttag ctttttgcat ctttaaattc aaaatcgttg cttcatccaa
    30181 gtcattcgaa gcatagattt ttgcttccgt aaatccggct gcatctaatt gttcccgaac
    30241 acgcttagat atataggcca tatctccact gtcgatgcgt acgccttgga agttgatttt
    30301 gtcacctaat tctttcgcca cgcgaatcgc acttggcaca cctgatttca acgtatcata
    30361 agtgtcgact aagaacacgc agtctttatg ggttttagcg taggccataa atgcttcata
    30421 atcatttcca taagattgga ctaacgaatg agcatgggtc ccgctagctg gtatcccaaa
    30481 tatcttccca gcacgtacat tgcttgttgc atctgctccg ccgatatatg ccgctctagt
    30541 tccccagact gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca atagtggatc
    30601 ttctccgatg acagatttga tacgagctgc ttttgtcgcg atcaacgttt ggaaattgac
    30661 catattcaac agtgctgttt ctactaattg gcagtgcgct aaaggccctt ccacttggat
    30721 caatggttca tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg ttgctttaaa
    30781 ttcaaaattt tgtaaatact ccaaaaaacc ttcaggatag acttctaatc cacgcaagta
    30841 agcaatatcg gaatcattga acgtcagatt ctctaaataa ttgactaacc gttccaatcc
    30901 tgcaaaaacc gcgtatccgt ggttaaaagg catttctcga aagtaacatt caaaaacagc
    30961 atgtaaatca gctcgaccta attcccagta tgtctgcatc atattgattt gatatagatc
    31021 cgtatgcagc gtcaaactgt cgtctgggta agtattattc atcttgtatt actcctcttg
    31081 gcaagttttc atttcctacc cattataaca aaaaaaatta ataggaaaag aaatcttcca
    31141 ttttttctga aaaagcacat gttttacgga tatttcttgc taaaaatgaa ctccatctgc
    31201 taaaactatc ctttcttatt ggtatttaac ctaaacgaga aagaatactt attttattca
    31261 atgtatccga gacacgaaga aattagcgtt ttctattttt cttacttgat aaaataagta
    31321 gattttccta ctacaatcgt agccgttaag ataccgatga ttgaacaaat ataaccaaat
    31381 gtctctacaa cataatcaat ggcaaaagtt tttgaagcaa taatacgtct ttcctttttt
    31441 gcttcttatc cccaaagaaa cggatataac ttattatgta ttgaattttt ttggaaaggc
    31501 gaattatgac aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat cacacgtttt
    31561 taaaaagata caaagtaaga aagcagctgt caatagaatg gaaacaaaaa tggcgagaca
    31621 tcaatcagtc tcgccttgtt ttttattgta tacattagat atggagcagg ttaccataaa
    31681 gatgattatt agtcgttatt aaatagaatc cttcgtattt ttttgttcta tcactacaat
    31741 atctttattt ggacgtttga attgtattcc ggcacgttct agctcctgtt tcactcctac
    31801 atggactttg acatcttctc gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc
    31861 ttgccttgtg atttttttct tcgtattgat catcacgact tgctttttat catatgtgtc
    31921 taccaatagc agttgtgttc gatctaacga aaataatttg taataattaa tagccgtatt
    31981 cccaaaaaag aattccacta ctttaggata aaatgtctta atatttaaat tactattcac
    32041 aggtgtttcg attagcttca tctcattcct cttttctata tagtcgattt agatccaatc
    32101 ttaagttatg ttaccatctt tcctagcatt aaggaatcgg ttacaattga acctttaaag
    32161 acaaaaggat ttacctttta tcacttaata gtataacata atttcatgag aaaaaaatac
    32221 aattttttct tgaccatctc gttacgtaaa ggattatgct tacatttgtc aggaggaaaa
    32281 ataatggagt acacaataaa gaaaatgtca gagatatcag gagtcagtcc gagaaccttg
    32341 cgcttttatg atgagattgg actgctaaaa ccagctagga tcaattcttc aggttatcgg
    32401 atttatggca aaaaagaagt cgatcgtctg caacacatct tattttatcg aacaatggca
    32461 ttcaagttgg atgatatcca agaggtctta gacaatccat cttttgacca ccagaaagct
    32521 ttaatcaagc atcagcaaat gttgctggaa aaacgggcac aaatcgatac cctcttgaca
    32581 actgtccaac agacacttga tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa
    32641 tttgaagggt ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg acaagagatt
    32701 cgacaaaaat acggaaaaaa aaaggttaca gcatccaatg aaaaatggat gaatatggat
    32761 aaagaaacgt atgaagaaat gcaaactgtt gaaaagcaga tgttatttga tctcactctt
    32821 tacttaaaag aaccagaaga tgaaagctta gcagaccgta tcttccaagc tcataagaag
    32881 tggttatctt acagctggcc tgaatatcag ccaaaagcgc ataaaggact aggaatgatg
    32941 tacgaagccg atgaacggtt cactgcatat tatgacgagc gaagtggtca tggtgcagca
    33001 aaagctttaa atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa
    33061 gtacacgtaa aatgacctaa taacaaaaca gcagacaccc aagttagatt tttatgctaa
    33121 cttttggtgt ctgctccatt ctttcacaag ttttttcatc ctactttaaa tatattcatc
    33181 gttgaataaa cgtataaggt attttcactt tatccttttt ttctgtaaga aacaattgaa
    33241 atgccatttc tccgcaagct tccagatggt ggtcgatggt tgggaaacca agaagctcgc
    33301 tgatcagcaa attatcgcgt ccaatcacca ctggtgtctt atcttgcaga tagctttgca
    33361 agatgcctgc tgcaaccata tccgaatttg taaagacacc atcaactcca agctctttaa
    33421 aaaagaagcc tgcttgtttt cctttctctg catctataca atcccaaaaa acaagtgatt
    33481 catcaaaaga tggaaaaagt tgatgacata gctgcaaagt gatttttgag ttataactta
    33541 gcctgccact tcgccccaac gtgatgccca attgctttat accttgctgc ttcaaataag
    33601 taagaccttc tgtaattgat tgttctcgat caataaagac acagcttgcc tgttttccct
    33661 tgatttcttc acaaaaaact agaggtccat atttttgata tgccaataaa tcatctagtg
    33721 tattggcacg agacgtaatg atcaatccat cgaaagcttt agctgcaaat tcttcaagat
    33781 accttcgctc tttttctttc tgatagcccg tcggtaaaag tgtcactttg taatcataat
    33841 caaacgctgc ttcaatgatc ccactgatca actgatcaaa atacggatga ttggtataag
    33901 gcagaatcac tccaatgctt ttcgtttttc cataacttaa ctgtctggca tttgcattag
    33961 ggatataatc gacttctttc atgaccgcaa gtatttcttt ccttttttcc tcatctacat
    34021 atggatgtcc attgattaca cgtgaaatag tagaaactga ataacctgtg agctttgcga
    34081 tatctcgaat gtttgccact gctgcttcgc cacctataca aaaagatttt cttgtaaata
    34141 gtctaccaca gctggaccgc attcaaaaaa ctgatacatt tttccttcat gcgaaaaact
    34201 gatccgctgc ccgccccaat atatatcttt tgtcaaattt tcgatttcat tcattggtat
    34261 ttcttttact atattcctat tcaacccttg aaaactgtac aacgttaata cggatccctt
    34321 gaactcgaca aacgcataaa aataattgtt cgttgtgcca tctgttatct ctacataagt
    34381 attcattgca agccctcctt ttgatacttt gagtatagtg tatgaaacgc gtctcatagc
    34441 aaggagtttg ttttgccaaa gaaaagtttt attcctcttt cattattacc aaagtctctt
    34501 attttaaatt gaatacatta taatgatact gtaaatatat ctttattatt agtgaatgga
    34561 tatttacttt taatagataa aatattataa ctattaaaaa taactatttt cattataatt
    34621 acacacaaaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    34681 gtataaacag cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
    34741 gaaaagcaat ttccgtgaat ttcttcttat ttcttggagt taaacgcttc tgttccagcc
    34801 tctttttatc tgtctttcac taatgatcag ttgtagataa gcaagttgct ttttcttaca
    34861 tcataccgcc catcattgat ggatccattg caggagctgc tggcgctgct ggttctggtt
    34921 tatctgcaac gactgcttct gtcgttaata acaaggcaga aacggaagcg gcattttgca
    34981 aggcagaacg tgttacttta gttgggtcta cgatacctgc ttcaaccatg tttacccatt
    35041 ctccagtagc agcattgaaa ccagtgccta gttccacgtt cttcaattta tcaacaatca
    35101 ctgatccttc ataaccagcg ttttcagcga tttgacgaat tggttcttct aaagcacgaa
    35161 caacgatctt gatacctgtt gccacgtctc cttctgcttc tacagcagaa actttgctga
    35221 taacgttcac tagagctgta ccaccacctg agaccattcc ttcttctact gcggcacgtg
    35281 tagcattcaa tgcatcttca atgcgtaatt ttaattcttt caattctgtt tcagtagctg
    35341 caccaacttt aacaacagct acaccacctg ctaatttagc taagcgttct tgtaattttt
    35401 cgcggtcaaa atcagaagtt gtttcagcaa tttggttttt gatcaattgc acacgagctt
    35461 cgattgcttc tttttctcca gaaccttcta cgatcgttgt attgtcttta tctacaacaa
    35521 ctttagaagc atttcctaag ttttcgattg ttgcatcttt caattctaaa cctagatcgt
    35581 cagtaattac tgtaccacct gtcaaaatag cgatatcttc aagcattgct ttacgacgat
    35641 cgccgaaacc aggagctttt acagctacta cgttgaatgt tccacggatt ttgttcaata
    35701 caagagttgg caatgcttca ccatctacat cgtcagcaat aatcaacaat ggacgtgatt
    35761 gttgcaagat ttgttctaac aatggcagaa tatcttggat gttagagatt tttttgtctg
    35821 tgatcaagat atatgggttt tctagaacag cttccatttt gtcgttatct gttaccatat
    35881 attgtgataa gtaaccacga tcaaactgca taccttctac aacatctagt tctgtttcga
    35941 tcccttttga ttcttcgatt gtgatgacac cgtcgtttcc aactttttcc attgcatcag
    36001 caatcaaatg tccaacttta tcagaacccg aagaaacagc tgctacttgt gcaatggctt
    36061 cttttgaatc aacaacagtt gagatattat gcaattcttc gactgctgct tttgttgcca
    36121 attcgatccc gcgacgaatg cctagaggat tagcacctgc agttacgttt tttaaacctt
    36181 cgcgaacgat tgcttgtgtc agaacagttg ctgtcgttgt accgtctcct gcaatatcat
    36241 ttgtttttga tgctacttct gatacaagtt ttgcacccat gttttcaaaa tgatcttcta
    36301 attcgatttc ttttgcgatc gtcacaccat catttgtgat caatggagag ccgtaagatt
    36361 tttctagtac gacatttcgg cctttaggtc ctaatgttac tttcactgta tctgctaatt
    36421 tatctacccc gcgtaacata gctgcgcgtg cgtcttctgc aaatttcaat tcttttgcca
    36481 tgattgattc acctcattga ttatttttga ttattttttt atcttccaaa tttactcaac
    36541 aatcgccatg atatcttttc ctgcgacgat cagatattct tttccttcgt attttacttc
    36601 tgtaccggcg tatttttcga acataacttg gtcgcctgct tttacagcgg caggaacttt
    36661 ttcaccgttt tctaacaaac ggccttcacc gactgcaaca actgtcccag tttggggttt
    36721 ctctttagca gcagaagcta aaacgattcc tccgacagtt ttttcttctt cttttgcgac
    36781 ttcgataatg acgcgatcac ctaatggttt taacacgata aatccctccg ataactcttt
    36841 ttttaatctg ttagcactct taaccttcga gtgctaacct acatttttaa taatactcat
    36901 tctccctctt ttttgcaagc ttttaaatca aagaaaagaa attttattct gacagctgct
    36961 tattgaaatt atgatataat tgaaatcaat cgatataagc ttgccttgca tgcttgttat
    37021 cccttaaaag gaggaattac atgtctttaa aaaaatatag cttcctcagc attttttgct
    37081 atggtctaat ttttatctca cccttgatcc tagcttcgat aggacttgta aaatctacgt
    37141 ctgaattgat cacagcaact gcagtcatgt atatactagg cgccgttgtc ctagctattt
    37201 tttattttaa gcaacgtgaa cccttagcga tcgaatcagc ggtaaaaaaa gcttctgctc
    37261 caaagattgt tatttatgga ttggttggaa tctttgtcgc attgattctg caaagcatcg
    37321 ctgtcatgtt agaatctgta ttgtttggca aagcaacccc ttccgagaat acacaaaata
    37381 tcatccagat gatcatggaa gcacccgctt ttattttggc gacaactgtt gctggaccca
    37441 tcatggaaga gttcgtattc cgccgcagta tactggggat catcagtcga tattcaaatt
    37501 tttgggtcgg agcagtgatc agctctttgc tgtttgcgtt tgctcataat gatggtcatc
    37561 tgctaatcta ctttttccta ggtttcttct tcagcctaga gtacaaagca actggtcgga
    37621 tctggacatc gatgatcaca cacgtaggaa tgaacacatt agttgttctt gttcaattag
    37681 ctgtccaaaa agggctagta taaacatgac tataaatact cctgtcatcc tagattggtg
    37741 attttcccaa tctaaagcga caggagtatt tttgtttttt agtcgttcat cttttctgct
    37801 gctccatcat tttttgtctg tctttccttt ttgcagtttg cagaatctat ttttttcaaa
    37861 aatgggaaat aaatgaaaaa actaattgac aacacgatac cttgtaaaat cgctgcaggc
    37921 actccgccta ctaagaaacc cgagaggatc ggcggtgttg tccaagggac ttggactgct
    37981 gtaaataaag gaacgaatcc aaaatataaa gcggaatagg taatcaatcc tgaaacgacc
    38041 ggtgcgaaaa taaaaggaac agccatcatc gggttcatca cgatcggtgt ggaaaaaata
    38101 attgattcat tgatgttgaa aaaggctggt aaaagagcaa gatttcctaa ttctctgtat
    38161 ttcttcgatt tcgcaaaaac cgtcataaaa aatacagccc ctaacgtcaa tccagcaccc
    38221 gtaaccgtca tgaactgatc gagaaattgc tgagtaacga tatgtccgcc atttgccacc
    38281 gtcaactctt gtcctgaggc taagatttct gcatttgcta atgaattcgc ttgcaagacg
    38341 ggactaacca acccattgac catcgcagat ccatgaagac caaataacca taaaaacgga
    38401 acaaaaaaac ctaataaaag cacaccaaac aacgtgtcta tgaacgattg aatgggaagc
    38461 tggacatatg tccatactcc ttgaacaaca gtcattctga acaaatgttg aaaaaccgct
    38521 gaaccgataa gcgtaaaagt gactataaca atgattggaa tcaatgaagt aaaagactgc
    38581 caaacatttt caggtatctt ctctctttgc ttccaccctt tcgcaagtct tactaaacgt
    38641 gcatagcccc atccgatgat gagcccagtt gtaactgctc ctagtatccc ttcagcacct
    38701 gtccagacag tatctatctg agtatttagt ggctgaataa gcagtagata agcagacaag
    38761 gctgttatgc cgactggtaa gcgatcgatg tcttcttctt ttgcataagc gtatgcaatg
    38821 gcaaaaacga tccaaaaggc tatcacatca aatgtagcct ggtatacttg tgtaaaatac
    38881 atagaaaatc ctgttttgtc tatccactga gcgatgtaat ccacaggaaa atgagcaaat
    38941 aagaaaaaca gcgagccaac aataattatt gggatcgtgt aagtcattcc attacgcaaa
    39001 gcagttaagg gtctaagatt cacaacagat gttatcattg gtaaaaaggc acgatctaac
    39061 cactctttca tttcttatct ccttcaactt ctatttttat caatcagttt tcttaattta
    39121 actataccca atgatcacga accatgaaag gaatgcgcac tgagcttttc aaaaatcgga
    39181 ttctttgacg aatattcagc aactgcttac aatggacagg aataaaaaag gaggtttttt
    39241 atgaaaattg cactcattgg agctactggc catgcaggtt catttatttt agacgaagca
    39301 ttgtccagag atttagacgt gacggcaatc gtgcgtcatc cagaaaaatt gccatcagat
    39361 gttccgttta tacaaaagga tttatttgag ttgacaacta ctgatcttgc atcttttgat
    39421 gttgtcgttg atgctttcaa cgctcccaaa ggaaaagaag aaatgcatca aacttctttg
    39481 tcccatttga ttgagatgct tgaaggaacc gatacacgct taattgtcgt tgggggtgcc
    39541 tcttcgttat tcttagatac agaaaagacg actcgtatga ttgaccaagt acctaaaaat
    39601 gcacccttct atccaacagc tttcaatatg tacgaaggat tgttaaaatt gaaacaatct
    39661 aaaaatctac attggactta tatcagtcca gcttctttat ttgatccaca tggcaaacgt
    39721 acaggtactt atttgttaag tgatgattat ttacaaagaa acgctgctag cgacagtacg
    39781 atcagcatgg cagattacgc gattgcatta gtagatgaga tactggatga gaaacatgaa
    39841 aagcagcaca tcagtgtagt cagtcgataa tacggttatt tttttaatat gctaaaaaaa
    39901 taaaaataga agataaaacc aaccctgatt gattttatct tctgcttttt atatttttat
    39961 tgattgaact gtttaaatcg aaaatagctc agttgcttca tctggatctg tatcatagat
    40021 ttcaaaatca tgtccaacgc caaaatcttt ttctttcaaa acattttcca ttgttaaatg
    40081 agcaagttct ttcatattgt tttccttgct aagatggcca agatagattc gtttggtccg
    40141 attaccgatc acatcagtca tgacaagtgc accgtcttcg ttggaaagat gtcctctatc
    40201 tcctaaaata cgctgtttta aattccacgg ataaggtccc atgcgtaaca tctcaagatc
    40261 atgattactt tccatcaaat aaccatcggc attttcaatg acacctcgca tataatcact
    40321 gcaatatccg gtgtccgtca acatgacgaa tgaccgattt tctttatgga aacgataaaa
    40381 ttgcggagcc gctgcgtcat gggagacgcc gaaactctca atatcgatat cgccgaatgt
    40441 tctgactttc cccatctcaa atagatgttt ttgttccact ggtacatttc caatcagtgg
    40501 ttccatcgct tcccaagtct gctgattggc aaaaacatcc aaatgatact tacgagccaa
    40561 gacacccacg ccatgtatat gatctcgatg ttcatgcgtc acaagtattc catctaattg
    40621 gtccggtgtc cgatcaattt gagccatcaa ggaagtgatt tttttaccgc taagtcctgc
    40681 atcgaccaaa agccgttttt tttcactttc gatataaagt gaattacccg aacttccact
    40741 tgctaggata ctgattttaa aagctggttt ttcttcagcc atttgactcc tacttccctt
    40801 cttactttgt aaactttttt cattatactc gcttttttat tgatcttcca cttttggtac
    40861 agtattgttt gtcacgatcg tattgctgaa agcgttgatg ctctcaattt ggaccacttt
    40921 atcgcttgtt tcgatcgcta catgccaaac aggaacatac acattttttt cccggacttt
    40981 cagaatacag gaataagcta gctgacgcca taaaattttt gatcctcttg gtattttgtt
    41041 attgatgtac aatgtattga ttgcttcctc ttccgtatac agttccgtct tttcgcgcag
    41101 ctgctcgata tcagataaat gcgtctgcat gtatttcgtg atgctataat cattatcgtt
    41161 tttttctaaa gaaagaacga tttttgctgt atcatcattg aatgggatgc cttcatactc
    41221 ttggcttgct tggatttcta gtggttcttc ttctaaatga gaaaagtttt tcgtgtacac
    41281 ataatcatta ccgaataaaa tattgtcttt gttagataaa aaatcttcca aggaactact
    41341 aattttatct ggatcgatca cgttcttttc agcatctgaa ctggataaag atttcgtcaa
    41401 aacatcaccg tctaaaaaca caccacttgc cagcaagccg cttttaccag ccttgcgata
    41461 attcgctaat gaagtgctaa gattggtctg ctccgcgcta agataatagc cttgcttgtt
    41521 ctcagacgaa atcttccccg tgtaagtgat atcgtcgcta gccagacgtt gttggattga
    41581 ttcagtctga ttagagcgaa tgacattgtt ttcttggtat accgcgtcat gataactgct
    41641 gaataaaaac acattcaacc ctaaaaaagc gacgaaaaat atccattcga tttttttgaa
    41701 atccaattaa ttcgcctcca atccaggtaa tttttccaga aggtcgttag ctgaatacca
    41761 gttatcttta tatttcacat accattcagg taagagatca acgacacgat tcgtttcttt
    41821 gacattttgc catgtatagc caacgatcac tgaaccaatc ttgttgctat ctgctccctt
    41881 agcttttaac tgctggatga tttcattcgt acttgcaagt tctacttctt cgtctgaagg
    41941 tatcggcact tgaatcgtat tcaaactggt ttggatattc acttgcacag aaccattcgt
    42001 ttcaccgcca acttccacac caatctgtcc tctgctgtct gtcccaaaaa ccgggaagcc
    42061 ttccacgaat atccgataat cgatcgtatt gtcactgcgg tcaaaatatc tgaggttccc
    42121 taaagaactg cctaatttgc taatataggg gaaactttca gaaaatacac tagcattttc
    42181 ttttttctct ggcagctctc cacgaaaatc aactacttgc gtttccgaat ggatagacat
    42241 tgtttcattc ccaccgtaaa aaatcaattc attgctttca tcattatttt tcacgctatc
    42301 tggtttctgg aaaaaagcat ttctaaaaag tgtatagggt tgctgtgaca ggatataact
    42361 atacttcttc agtttgatcg aatgtttcgt agcatattgc gtgtcgatcg tcgatggttc
    42421 tgttgtcatt tctgtccatt ttgtgtcttt tgctgttaat tctttattga tatctgtcca
    42481 attcaacgaa atatttgctt catatacgag atggttcgaa taattcaaaa atcggatttt
    42541 tttatttcct tgatcgaatt gtattctagt aaaatacata ttttcgcttt cttttgccaa
    42601 gttcgacaca tctaaatcta aatcaaatgc ttccttgtat tctcttagca aaaaaggcgc
    42661 attataggcc aactcaaaac cagagttcag atttttcacc tgttcaaact gtgatttgtc
    42721 tcctgaaact acttcagaaa gctttccaaa tcttgcttta tcgatgatca attgcaatcg
    42781 gtacacgaga ttttcactgt tggtttcttg gatcattccc gaatggtgcc atgtaacatg
    42841 aagcggaaga aagacttcgg aagtttttcg attgctttga gactcgatca tttgagtgtt
    42901 tgtctcatct acgttgattg aactttttcc tgctgggctc agccagattg cataagagaa
    42961 gtatatgctt aacgcaacca tcaaaatgag tcctatacga acgacttttt cagagattct
    43021 cattcccacc attcctcctc ataaggttca tatggcagcg agatataaaa agtcgaccct
    43081 tttccttcct tgctctctgc ccaaatagcg cctcgatgcg ccttaatcac ttctctagaa
    43141 atagctaatc caagtcctgt cccaccttgt gctcttgcac gtgctttatc tactcgatag
    43201 aaccgctcga acactcggtt cagatctttt ttaggaatcc ccaatccttg atcggtgata
    43261 cttaaaatca cgttattatg cgtttctaat aagcgacaag taatcgttcc cccatctggt
    43321 gaatacttga tcgcgttatt catgatatta tcgactactt ggatgatttt atctgtatct
    43381 acttcgaccc aaagatctct ttgcgtaaat tcacgacgga tcttataatt tttttcctga
    43441 tttcccacca tcatatcaaa ccggtcaaga acaaaattca ctaactcgtt gaaattgaca
    43501 tattctaatt gaagctgggt attacctgta tccatgcgag atagatttaa gaggtcattg
    43561 atcattcgaa tcattcggtc tgtttcatct agcgttacct tcaaaaaatt tggtgctact
    43621 tcctcgtctt tccacgcacc ttcgctcaat gcttcgatat aactacgcat acttgtcaac
    43681 ggtgtacgaa gttcatggga aacattagag acgaattcac gacgttcccg ttctgttttt
    43741 tcttgttctg tcacatcatg caagacagct actaatccgc tgataaaacc tgattcgcga
    43801 cggatcatag aaaagtctac tcgcaagatc atctgatcac tttccaatgt actggttgaa
    43861 cggtcaagta ccatttcgtt tggatcttct aataatttcc gcaacgtata ttcttcttcg
    43921 atatctaaaa gtgtcaaaat cgattgtccg attgcttcct cgtttttgat atctagcaag
    43981 gaaacagcca tatcattgat cgtaatgact ttacccctgc gatctgtcgc aatgacgccg
    44041 tctgtcatat gggatagcac gctatccaaa cgattgcgtt cagcttccat tgtatcttga
    44101 gcttcttcaa tacgttcagc taactgattg aaggtatcag ccagctgtcc aagttcatct
    44161 tgtccatgca ctttgacttt ccgactgtag tctcctctag cgattcgaat ggcttgttct
    44221 ctcatttcac cgatcggctg tgtaatcgaa cgagcaacta aaacagatac gatcattgaa
    44281 ataaatgccg caattagtga ggcagtaaag aaaatgactg ctgtgttatt gatttccgaa
    44341 tatttttgtt cgatattact tttgacgtaa agagccccga taacggcatc gccggtcggg
    44401 gacaggatcg gttgaacatt gatattcacc cgtttatcgt cgttatctaa agcaacataa
    44461 cgtttagtcg tgaaatcatt gatatcaaca taatcatttt tctttcccac agcgctttgt
    44521 tcattgacat cggttgttcc tcggatgatc cctttttcgt caacgacccg tatttcgatg
    44581 atatcgctag tcgcactgtt ttcaataaat cttttgatat tcgcatttac ctcttcatca
    44641 tctgcattat cttttcgatt caattccggg ctgattgttg ttgctaatga ttccaccgtt
    44701 tgattcatat ctttaataaa tgtattgatc gtggaacgtt ctaaaccacg tataaagtag
    44761 gcaccaataa tttcgatcga gatcaataaa atcagaataa aggtaatagc gatcttaaaa
    44821 ttgactgatc gaaaaaagcg gacttttctt ttcatccaat tactcctgtt ctgggttacg
    44881 caaatagtaa ccgacaccac gacgtgtcac taaataattt ggatggcttg gattatcttc
    44941 gatcttttca cgcaaacgac gtacagttac atccactgtt cgtacatctc caaaataatc
    45001 ataaccccaa accgtttgta ataaatgttc tcttgtcatc acttgtccaa ggtgcttcgc
    45061 caagtaaaag agtagttcga attcacgatg tgttaactcg attgtttctc ctcgtttcgt
    45121 caccatgtaa gcttctggat ggatcgtcaa atcaccaatc gtcaattccg caggtgctgc
    45181 ctcttctggt tccttcgcag ccgtggcacc gcgacgaaga tttgctttga cacgtgcaac
    45241 taattctcgg ttggaaaatg gcttggtcac gtaatcatcg gcgcctaact ctaagcctag
    45301 gactttatca atttcagaat ctttggctgt taccataatg atcggcatat cataagtttt
    45361 gcgtacctca cgagcaactt ctaagccgtc cattttaggt agcatcaaat caagtaaaat
    45421 caaatcaggt tctacctcag caactttttc caaggcttct tccccatcat atgcagtata
    45481 aacgtcatag ccttctttgg ctaaattgaa tttgacgata tccgaaatcg gcttctcatc
    45541 atcgacaact aaaacttttt tcataaagag ccacctctta tatattattg tcataccttc
    45601 tcattatacc gtatttctgc ttttgtttca tctttgccta taaaacaaaa gagaaaatta
    45661 aaatgaaagt gcaaaatact ttattatttt ttgttctaac ttcaataaaa aaggaaaaca
    45721 ctgattttca cctcagtgtt tccttttttt gatttatcct tctaaaactt gtacaccgac
    45781 acccaataat cccggaccag tgtggactac taatgccgga gaaatctggc caaagtagat
    45841 ttggtttgct tgtgggaact ctcttttaag tctcgcttcc atttcaattg cttcttcttt
    45901 cgcatcccca tgggcaacag ctaagttgaa gacctttgca ttaccgattt tttctttgac
    45961 gtaagcaacc gttttatcca aacttttttt acgtccccta gcttttccta ctgtgtaata
    46021 aatcccttct tcattacatg agatgattgg gttaagcttt aaagcggttc ctacaataga
    46081 agcaactaaa ccgatccgtc cacctttttg aagatattcc aatgtcgcaa cattaaagaa
    46141 aacttttgtt ctttcgacta actcattcag attactgatc agttgatccc attctactcc
    46201 ttcttccagc cattttgctg cttggatcgc agaaaaacct gcaccaatac caatattttt
    46261 cgtatctagt acatatgctt ccaattcttc ttgctgttgt gcaattaaac gaaccacatt
    46321 atatgtcccg ctcaatccac tggaaattgt taccgcaagt accttttcat aaccgtcttt
    46381 tttgatttgc tcaaagattt ctgtgatcga ctcaccatcc ggcaaagatg taccagggat
    46441 ttcctctggc aaacgttgat agatctcttc aggggtaatg tcaattttat ctgtatatac
    46501 ctgatcttta taaataattt tcaatgggat catatacatc tcgtattttt ttatcaattc
    46561 ttctggaaca tccgtacctg aatctactaa aattgcgatt ttattgtttt tcatttgttt
    46621 tctcctcttt taaatattcg gtttctagaa caatcgtttt ttccgcaaga agtttcattg
    46681 caaacgataa agttgccgct cttacagcaa tatattctac aggggttgcc tggtcgaatt
    46741 tttgcggatg agatttacct tgtgctaatt ggctgaccat ccacacagct gcttcttgtt
    46801 catcgcaaaa tagattatag gcttcacgaa ttcctactgt ttttccttgg aacaatatcc
    46861 cttctttgat ttcaggaata gtcaatactt gtttcaacgt agtgattgca agtaaaaatg
    46921 ctacgtgttc tttgttatat ctttttttaa ctggtgctgg tatcaaacct agtttaacgt
    46981 aattgtttac catagaagag gtaagcaatg gatgcttttc tccttgtatc acaggtgaaa
    47041 gataacgatc aactaacgtt ctgacttggt ccatataaag ttctaagtca gggagttctt
    47101 cccagtgagg cagacggaat gtttctagtt ccttccccca ctccattaat tgctgacgca
    47161 ttttttccat cataaccgct ccttttttca tttcataaac atgatatcag acttatgata
    47221 aacattcaat ctagttgctt taactaggtg atttgattag cgggattcca tgaaaacaaa
    47281 aaacaagaag gctaagcctt cttgtctttt atcgttaaga acaggagacc cgccggggag
    47341 acggcgggaa aggagtaaaa aatgaaaaaa gtgttagggt ttgttctctt gcaagaactt
    47401 tgtttgttgg tatgcttata taatagcgag caaatgtgaa taatctatga atgatttcct
    47461 ataaggaagt aaagattttt ctgattttct ttaaatgtag tcttgcattt taaaattctt
    47521 agtgctacta ttattaagta ctttttgtac gggccgttag ctcagttggt agagcagctg
    47581 actcttaatc agcgggtcgc gggttcgagc ccctcacggc ccattgggtg ccaaacccac
    47641 ggaacaggta aatgttggtg cgaaagcatt tcgaaaacat ttacgctgtt ctttttttat
    47701 ttttcaaaat aactagggct tttt
//
LOCUS       DACCCR010000014        46686 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCCR010000014 DACCCR010000000
VERSION     DACCCR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.014"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            101..571
                     /locus_tag="IVK93_000777"
     CDS             101..571
                     /locus_tag="IVK93_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP6386088.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="IVK93_000778"
     CDS             793..1896
                     /locus_tag="IVK93_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6386089.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2117..3076
                     /locus_tag="IVK93_000779"
     CDS             2117..3076
                     /locus_tag="IVK93_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6386090.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3246..3926
                     /locus_tag="IVK93_000780"
     CDS             3246..3926
                     /locus_tag="IVK93_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6386091.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            4016..5050
                     /locus_tag="IVK93_000781"
     CDS             4016..5050
                     /locus_tag="IVK93_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP6386092.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            5064..5399
                     /locus_tag="IVK93_000782"
     CDS             5064..5399
                     /locus_tag="IVK93_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386093.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            5383..7863
                     /locus_tag="IVK93_000783"
     CDS             5383..7863
                     /locus_tag="IVK93_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386094.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            7919..9478
                     /locus_tag="IVK93_000784"
     CDS             7919..9478
                     /locus_tag="IVK93_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6386095.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            9475..10839
                     /locus_tag="IVK93_000785"
     CDS             9475..10839
                     /locus_tag="IVK93_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6386096.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            11010..12080
                     /locus_tag="IVK93_000786"
     CDS             11010..12080
                     /locus_tag="IVK93_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6386097.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            12095..12763
                     /locus_tag="IVK93_000787"
     CDS             12095..12763
                     /locus_tag="IVK93_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6386098.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            12783..13361
                     /locus_tag="IVK93_000788"
     CDS             12783..13361
                     /locus_tag="IVK93_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6386099.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            13762..14673
                     /locus_tag="IVK93_000789"
     CDS             13762..14673
                     /locus_tag="IVK93_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6386100.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(15051..15968)
                     /locus_tag="IVK93_000790"
     CDS             complement(15051..15968)
                     /locus_tag="IVK93_000790"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP6386101.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            16170..16607
                     /gene="zapA"
                     /locus_tag="IVK93_000791"
     CDS             16170..16607
                     /gene="zapA"
                     /locus_tag="IVK93_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP6386102.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            16665..17213
                     /locus_tag="IVK93_000792"
     CDS             16665..17213
                     /locus_tag="IVK93_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP6386103.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            17328..19688
                     /locus_tag="IVK93_000793"
     CDS             17328..19688
                     /locus_tag="IVK93_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP6386104.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            19775..20089
                     /gene="trxA"
                     /locus_tag="IVK93_000794"
     CDS             19775..20089
                     /gene="trxA"
                     /locus_tag="IVK93_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP6386105.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            20271..22049
                     /gene="uvrC"
                     /locus_tag="IVK93_000795"
     CDS             20271..22049
                     /gene="uvrC"
                     /locus_tag="IVK93_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP6386106.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            22194..23093
                     /locus_tag="IVK93_000796"
     CDS             22194..23093
                     /locus_tag="IVK93_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6386107.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            23086..24348
                     /locus_tag="IVK93_000797"
     CDS             23086..24348
                     /locus_tag="IVK93_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6386108.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            24509..26407
                     /locus_tag="IVK93_000798"
     CDS             24509..26407
                     /locus_tag="IVK93_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP6386109.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(26737..27456)
                     /locus_tag="IVK93_000799"
     CDS             complement(26737..27456)
                     /locus_tag="IVK93_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP6386110.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            27793..28125
                     /locus_tag="IVK93_000800"
     CDS             27793..28125
                     /locus_tag="IVK93_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386111.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            28112..30115
                     /locus_tag="IVK93_000801"
     CDS             28112..30115
                     /locus_tag="IVK93_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP6386112.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            30134..30604
                     /locus_tag="IVK93_000802"
     CDS             30134..30604
                     /locus_tag="IVK93_000802"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP6386113.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            30625..31209
                     /locus_tag="IVK93_000803"
     CDS             30625..31209
                     /locus_tag="IVK93_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP6386114.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            31224..32210
                     /locus_tag="IVK93_000804"
     CDS             31224..32210
                     /locus_tag="IVK93_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP6386115.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            32200..32511
                     /locus_tag="IVK93_000805"
     CDS             32200..32511
                     /locus_tag="IVK93_000805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP6386116.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            32533..34314
                     /locus_tag="IVK93_000806"
     CDS             32533..34314
                     /locus_tag="IVK93_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP6386117.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            34307..35683
                     /locus_tag="IVK93_000807"
     CDS             34307..35683
                     /locus_tag="IVK93_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP6386118.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            35690..36325
                     /locus_tag="IVK93_000808"
     CDS             35690..36325
                     /locus_tag="IVK93_000808"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP6386119.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            36355..37710
                     /locus_tag="IVK93_000809"
     CDS             36355..37710
                     /locus_tag="IVK93_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP6386120.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(37902..39296)
                     /locus_tag="IVK93_000810"
     CDS             complement(37902..39296)
                     /locus_tag="IVK93_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6386121.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            39388..40359
                     /locus_tag="IVK93_000811"
     CDS             39388..40359
                     /locus_tag="IVK93_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP6386122.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            40356..41138
                     /locus_tag="IVK93_000812"
     CDS             40356..41138
                     /locus_tag="IVK93_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP6386123.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(41307..41678)
                     /locus_tag="IVK93_000813"
     CDS             complement(41307..41678)
                     /locus_tag="IVK93_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6386124.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            41812..42255
                     /locus_tag="IVK93_000814"
     CDS             41812..42255
                     /locus_tag="IVK93_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6386125.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(42298..43107)
                     /gene="yidA"
                     /locus_tag="IVK93_000815"
     CDS             complement(42298..43107)
                     /gene="yidA"
                     /locus_tag="IVK93_000815"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP6386126.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(43126..44493)
                     /locus_tag="IVK93_000816"
     CDS             complement(43126..44493)
                     /locus_tag="IVK93_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6386127.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(44477..45307)
                     /locus_tag="IVK93_000817"
     CDS             complement(44477..45307)
                     /locus_tag="IVK93_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP6386128.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            45470..45907
                     /locus_tag="IVK93_000818"
     CDS             45470..45907
                     /locus_tag="IVK93_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP6386129.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            45983..46603
                     /locus_tag="IVK93_000819"
     CDS             45983..46603
                     /locus_tag="IVK93_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP6386130.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        15353 a         8012 c         9911 g        13410 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcatac ttcgccgatt gtaaaattaa
     2041 gctagacaac taaaaaaagt catttgtgct acactcaaaa tagttccgac gaaagaatta
     2101 taaggagtga gtacaaatga cctataacca tcttacacca actgaactcg taatgataga
     2161 agcttatttc aatcaatcac aacctgtttc aaaagtagcc aatctgttac aacgttctcg
     2221 acaaactatt tataaggtct atcgttttct taaatctggt ggaagcgcaa tgagctacta
     2281 taaacaatat aagaaaaaca aacgtaactg cggaagacat ccgattgttt tacctgatga
     2341 gcaacaggaa tacattcaaa aaaaggttgt tcaagggtgg gctcctgatg tccttattgg
     2401 tcgagcagag tttcctattg cttgttcggt acgcacactg tatcgaatgt ttaaagataa
     2461 aacctttgac actcacgatt taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga
     2521 aaaaagagga aaacaagggt ttcgccggtc aatccgagac agaaagacag actataccca
     2581 gtttgatcat gaatttggtc acctggaagg tgacactatc gtgggtggaa aacataaaag
     2641 cgcggtcatc acattagttg aacggctatc taaagtcatc attaccttga aacccgaagg
     2701 cagacaggct aaagacattg aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa
     2761 cttatttaaa tccatcacat ttgattgtgg aaaagaattc tcaaattgga aaaagattag
     2821 taataccaac gatatttcaa tttattttgc ggatccagga acaccttctc aacgaggatt
     2881 gaatgaacat tcaaatggct tattacgtaa agatggtttg tataaagcta tggattttaa
     2941 tggtgtatct gaaaccttta tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa
     3001 atcattacac tatcggacac caatggaagt atttttgagt tacgtcacaa aagatgattt
     3061 gtctaactta atttgacaaa cgaaacttaa taaaaaagga agaatattca tgtaaacgat
     3121 tttattgttt ttttctcttt tatacaaaaa gggtcataat ttcaagtaag cggatgaatt
     3181 catgaggatt ttttgcatac tgtctctact atacttaggt cactaagaaa aaggagtgat
     3241 ttattatgaa aaagaacatg attgggacat tattactttc aacacttctt ctaggcggct
     3301 tggcaacacc agcttttgct gagggtcaag cgacttcaaa aggagatatt acctttactg
     3361 aaccaacaaa taccgtggag cctcttaacc ctactgatcc ttcaaaacca gtagagccag
     3421 cagatccgga aaatccagca actggccaga caggtccttt aacactggat gtagttcccg
     3481 aattgccatt tggtacacac gagattgaaa gcggaacaaa aacgtatcaa gtagacgctt
     3541 caaaaaacga tacaccttat ctccaagtat cagatcgtcg aggtgtaggt gcagatggcc
     3601 aagcacaagg gtggaacgtg acagtttcgg tttcagattt tgtaaacggt tcacaagttt
     3661 tgcaaggcgc tgaattagat tttggaacat ccactgttaa aagtacgtca gataacgaat
     3721 ccactggacc aactagtcaa actgtaactg ggttgagtaa agcaagtgca gcgacaccaa
     3781 tatttacagc agcaaaagat caaggtctgg gaacatggct ttctgtttat gatccagcaa
     3841 atatcacact gaaagtgccg aaagcagcag ctggaacatt cacggctgat ttgacttgga
     3901 acctcgtggc tggcccagta gcatagagta tgaaataaat tcaacaaact gcggtgaaaa
     3961 tactataggt gaaggatttt ttaccgcagt tgttgttgaa aggatgtggt ggacaatgaa
     4021 tcaaagtaaa cgactgtttg tttcgttttt ttctatttta agtctatttt tcatttttcc
     4081 gtcaatcagc caagcaaagg attcagcagg agactttgga atcaaaccgg tatttccaga
     4141 gaaccaaata gataaagcaa tcggttattt tgatttatta gtggcaccgg aacaaaatca
     4201 gacactagaa gtaattatca gcaattcttc agatgaagaa cgaacatttg aggtctctgt
     4261 caatccggcg gtaacgagtg atggtggaac gatcgattat agtcaaaaaa acccaacgtt
     4321 agatgagaca ttgccgtttg atgtccgtga cgttttgctc attgcgaaaa aggaaatcaa
     4381 tgtatcagca catgcagaaa caactgtacc tatagaagtg aaaatcccag caaaaagctt
     4441 taagggacgt gtattagcgg gtattcatgt ttcacctaaa gaagaagctg aaacagaaaa
     4501 tgctacagaa ggagcacaaa tcaaaaatcg tattgcttat aatctcgcag tcgttttaca
     4561 agaaagtcaa gagacgatag aaccggatct gaaactttta tcaggagatt tggacgaggt
     4621 caacgcaaag ccaactgttc agcttcgttt ccaaaatcct cagcctagga ttatcagcaa
     4681 tttgatattt acgagcaaga tcttttatga aaatcagctt tatattgaaa atacgtctaa
     4741 tgcattttta gttgcaccaa attcgaattt tcacttgaat cttgatttag ctggtgataa
     4801 agcaaaagcc ggcgattacc gcgcagaaat catagcaaaa tcaggcgaca gtaatgaatg
     4861 gcgatttaca caaaacttta cgatcaaaaa agaaaaagca caaaaagtaa atgaaaattc
     4921 cgtttttgca gtacaagaac aatctttccc atggctttat gcaggattgg ttgctgcagt
     4981 tctagtgtta tggttgattt tgctgttttt gtgggtccac aagaaaaaga aaaaaggaga
     5041 aacaacatga tgaggtgatt tcgttgaaaa catcgatctt attgggaggg attattgcaa
     5101 gtttgctgtt cttcagctgg caagagccag tccaagcaga taccaaaagt tcaggtacga
     5161 ttacatttta tgatctaagt ccaattccgc atccaagtgg ttcagtcaat ggcgcatcaa
     5221 aacagcagac acagctaccg atttataaag aatctaatca acatggcgta ttcccggaga
     5281 cgaatgaaca aaagatccgt tttctttatt tgatcattgg tatagaatta tggcttcttg
     5341 cgtggttgtt gttatggaga agaaaggaaa aggaggatga acatgttgct acaaaataaa
     5401 ttttcatttt ttcgctcctt cttttttact tcgctttttg ttttaagtat atttatcttt
     5461 tcaccttcgg cagaagctgt tggagcaacg cctcctgcca tttcttatga tccatacacg
     5521 tatgaaaata tcgtacctcc aaatggtgga cgtagtgcag tcaatggcgg gaagcttgtc
     5581 caactaactg gggctaacca attgcctaaa ttgactgcta cttatttgaa cgatggttca
     5641 aatgatcctt ggaaaggtgg cacggtccgt tttcattggg aaagccatgt gaaactaaca
     5701 aatgtacttg atttggtgaa tatcactgca caaggatttg catttgtggc gatcatcaat
     5761 ttgccggaac atgtatcaag cagtgatgta ttaaaagcaa ttgactggaa caacgcctac
     5821 ttgtatattg ccggtgcccc catcaaggtt actagcggaa gtttattatc agcaggaagc
     5881 cataccttgc gttttgggat ggggtcgtgg gatacaaata ctctattgac agatattatt
     5941 gctattatta cgcgaggcga attcaacgtg gacgatatcc cgcttcaatt tgatgttaca
     6001 gctaatgtcg cgaagatgac agaaaacgga gataaatatg atacgagtcc aaacaaaatt
     6061 ttgaccaagg gtaaattaca accttctatg tctaaaaagg ccgttttttc tgttgatttt
     6121 tatgattcga ataatattat ccaagatcgt atgttcaatt cgattttagc tccagctgca
     6181 ggtagatttt atcctaaatt aactggcgga aacttgaatc caggagcata tgcattttct
     6241 gcaagtgcag agataaatac gtggaatagt tacatctcac caagtgattc aacaggaaaa
     6301 tataatacat ttgctgatac cacagaatac gtggatggaa ataaccagaa ccttccaggc
     6361 tctaccaata gtcggacatt gacgatgggg ctgactgaag gacgttttga tcagttgcca
     6421 tttagtcgct ttgatcgcgt agtcaactat tttacaggaa aaaacgttac acaaggagca
     6481 atactgactc atactccagc aagcacaagt ggattgaatc agacagccaa tatcgtgtac
     6541 agtgggaaag atgcgcaagg cggattgctt tctcctgttg ctttaaaggt attggagcag
     6601 tacaagctag acggaacggt tttaccaaca aacttaacag ccgatagtca gtggggaaag
     6661 ctcctttctt ctgcatcgta ccaagtcacg gccaattggt cagcaactga actaaaaaag
     6721 ggttcgatcc attacaggct ttattcgaaa ggaacccaaa aattagcgac aggatggaca
     6781 gatcaacttt tccaaacgat ccaatcaaat aacgggggtg taaatacagc tacttctaca
     6841 ctacctgcat tggcttcagg gcaatattat tttgattatc gcttggtgga cgatgtactt
     6901 actaacatgt atccgtcttt tgcgtacaaa tggcagtctg aacaagctgg aatcaccact
     6961 cttcctcaaa taacagtggc taattttcca tctgtgagtg cagatagtca gctaaaaaac
     7021 cttagcagac aacctgagac aggagatccc ctgtctgctc tatcaggaga tacgattcaa
     7081 gaaaatgtga cattcacgtt aacaaaatct ggggatgcga taaccgacaa aaaaatcacg
     7141 atctcacttc ctgaaaatac tacttatatc aatggttcct taaaattaaa tggtacaact
     7201 atcgcagata ccggcattca acaaggagtt tctgtcccag cagatctatt gagcaaaata
     7261 ggagatacca ttcatcttac ttacaactat caattaaata ctgtagatac ttctttacaa
     7321 tccgtcagca tactgacaaa ggcagctgta ctttccagca atattacatt agcagatggt
     7381 gctaagcttc ctaacccagt tgtccaaaca tctgctaaaa caattcttgt ccccaaacaa
     7441 gaattaacat tagttaatgt accagatgat tttacatttg gtaacgatct ccccaaaccg
     7501 ttgaagacaa gttattatga atcaaaaggc gatttttctt ttgatgtgcg ggatactcgg
     7561 cttccaagca cgtctccttg gcaactgaca ggtacattga cttctttatt taagaatgat
     7621 caaggtcaag aattaagcgg aacaaaatta tacttcaatc attctggatc aaagcaattg
     7681 attcagcaag gtcaaaacac gttgatttat gagagtgatg gaacagctaa aggagaggtt
     7741 ctcgttgact ttcccgatac cgatggttta ctcttagagg taaatagcag tacgaatgct
     7801 caacctggcg caacgtatca aggcatggtc acatgggaac tgacagctgg gccaaccagc
     7861 taagtgatta gaatttgtat ggatctgtga taactgtcac agatcctctt tttcaaggat
     7921 gagaaggaaa cttctgctgt taaaatcaga aatgcttgaa ggagtggact tggaactttt
     7981 cagtttgctt gaaaaaaatg aacaacggca aatcaaactt ttccaacttt tattagagga
     8041 aaaaggagag gtttccgtta ataacgtact tacccgctta tcagttgatc gggctacctt
     8101 gaaagaagat ctgttttatt tacaagctaa cttggaaaat atcaaacaat tggaaattgt
     8161 tatccaagaa gggaatattt tattaatcag acatggaaat atagcagcag tcgatgttta
     8221 ttatcattgt tacttgcgct attctacaaa atatcagata cttctttatc tgttggaaaa
     8281 tggctcattt gatcggcaga aacttgtcat ggaacttaat atcagttcgg ctactttgtc
     8341 tcgcagagtg aaggaattaa atgattcttt aaatgagttt catctgaaaa taaaaagcgg
     8401 gcggctgacc ggtccagaaa gtcaaattcg ttatttttat ttccaattat tttggtttgg
     8461 acgtccctat cttgaaaaca aaaaagaata cagtgattta tcaggaaaca cttttttaga
     8521 tctgctgcaa aaagagttta gttttgctat tacagaagaa ggacgattga aatttgaaat
     8581 atggctttcc attatgaaga agcgaatgcg ctgtacgaaa aaatccaaac cagattatag
     8641 caatcttcct gttggattgt ttcaaacgac acaattttta ttggacctcc atacattttt
     8701 gacccgttac ttctttcaat atgcctttcc ttggaatcaa aatgaaacgt tggtttttta
     8761 tcttttcatg atcagtaact ttacattaga tacacataac tcgcatgtcc aggagttttt
     8821 gataagcgaa aaatacaaag acgacgtagt agctcaattg aatcgaacgt ttaaacaatc
     8881 gatagaagaa tatttctctt tttatgtttt tccagaagag atttcgcaaa agattgcgtt
     8941 gacagtgatg caaaatcacg ctcattttgc ctattttaga ggatggatga atatttttgg
     9001 ccaagaagga ttaaaacagc ggttaactga agtagaagat aaacaattcc tggttttatg
     9061 tgaaagattg gtcagacaaa catttgctat tgttgatttt actgaagtag acgaggaaaa
     9121 tgcccgcttt gaattatttg ctcgctatgc atctatttta cggacgctga tcaagcagtt
     9181 agaattacgt ctgcaaattg cttatgatct tccttatgaa aggctaatgg cggatatcgt
     9241 gaaagaaatc atccaggaac aattgctgaa tacgatcaaa tatgatttag tcgaatacga
     9301 gaaagaaaag cagtatgata tcattttgac tagccaattg aaagagtacc cgtcacagaa
     9361 gtccgctaaa gtttttgtat tcactagcaa cgaatataat tatgatttcc cctatttgaa
     9421 ccaattttta aaagaatgtt atttagaaaa attgaattta aggatgaata aaacatgaaa
     9481 atcgtgatca ttggtgcttc ccatgaaggc atcacagctg ccctaaatct aagaaaactt
     9541 caaccgaaag cagaagtttt actgatcgat gaagatcata aagatggact aggctatgtt
     9601 tcaaatggaa tcaaccttta tttaaaagga aagatacgta gtttagctga agttgcccat
     9661 aacatgcgga ctcttcaaga aagtggggca aaactgatca ccgaatggag agtaactgaa
     9721 cttgatccag acaaacatca gctcatcctc gcatccaaag aaggaaaaaa cgagaccata
     9781 acctatgata aattaatcgt agcgacaggt tcctctcctg tcaccttgta taaacaaatt
     9841 gaagctgaaa atgtctacac ctataaaaat ctagtgcaat ctaagcaagt acttgccgct
     9901 ttaaaagaag caaaagaagt agtgatcttc ggtgctggtt acattggatt ggaattagca
     9961 gatgctttga gaaataaagg atacattatt catctagttg attatatgcc gaatgtttta
    10021 tcaagatatt tcgataagga tatgatcaac agttttcaaa atcagctgca aactaagcaa
    10081 ataaattttt atccaaatga attcttgatc gattggaaaa aatcagaaga gaaagtagta
    10141 tccgtacaac ttctgtctca aacaataaag gcagatatgg tcattttttc tgcccaaact
    10201 cgtcctaata cgacattgtt aaaagaaaaa gtggctttgt atgaggatga gactgtgata
    10261 gtcaatgaat atctgcagac aagtgatccg gacatctatg cgattggaga tatcgtccca
    10321 gtcagttttg acaaaaataa acggcatctc tttttacctt tagttactcg agctgtacat
    10381 atggcaagag cagtagcgct aactttgagt ggtcagccaa ccgcttacga cctcagacaa
    10441 aagataacag cagcagtcat tatagactac tttttaggaa ctgtagggct gacagaagat
    10501 gaagcgccat tcttggaaca aaataccggt tcgtgttcag gagaatttga tctatttcca
    10561 caatacgacg aagaaaacaa aacagtaaat gcaaagctga tctaccatcc ggatacatta
    10621 gaaataattg gcggacagct gataagccaa gaattcttat tatcagattt gaacctgctt
    10681 gcggatatcg tgaaacacaa aactacgatt ccacagctag cagttgagga ttttggattt
    10741 ctgtctgaat acacctctcg ttttcactac ctcaatgagt tagcttttaa agtactagct
    10801 aagagagcaa atagcaatag agtagataaa agactgtgag aaatatcacg gtcttttatt
    10861 gtgtggggat ataataaata ttaatcaatc gagaataaaa aattcatata atgaaatagc
    10921 ggaagaattt tttgagcata atattcgcca aaaatttatc aagtgataaa attgttgtat
    10981 aaaatgatta tttcaggagg aggagttaaa tgtttttagc attaaatgaa attatgcatt
    11041 caaagttacg ttatgcgtta gttgcagggg tgatgttttt gatcgcctac ctggtatttt
    11101 ttttgactgg gttagcttat ggcttagctc aagataatcg aacagcagtc gacaagtggg
    11161 aggcagattc gattgtgctt tcgaaagatg caaattcaaa tctagggatg tccatgatta
    11221 ctaagaagat agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc caaacacctg
    11281 gagtcgtgac tagtaaagac agtacagaag aaggaaaaat caatgttagc ttcttcggta
    11341 tcgataaaaa tcaattcatc atgcccaatc ttgttgaagg aaaagctttt gataacgatg
    11401 atgaggcagt aggcgatatt agtttgaaag aagaatatgg tctagccata ggagatacag
    11461 ttaagttatc tggtagtgac aagacgttta aactaactgg ctttacggat catgccaaat
    11521 tcaatgtctc accagttctc tatacaacca tcaatgctta ccaagaaatc cgcttcgaaa
    11581 aagaagatac aagtgaaaat gcacggatta acgcaatagt tgtcagaggt aaaatcagta
    11641 atttacctga agatttagaa cagatcaaaa tttctaaatt catcaatgaa ttacctggat
    11701 ataatgcgca agtattgact tttggattta tgatcggatt tttgatcgtt attgcggcta
    11761 ttgtgatcgg gattttcatt tatgtactga cgatgcaaaa gattaatatc tttggtgtaa
    11821 tgaaagcaca agggatcaca ggaggattta ttgcgcgttc tgtagtggca caaacattca
    11881 ttttatcatt tgttggtatt cttcttggtc tattaggaac agtgggaaca tcccttgttc
    11941 tacctgatgc ggttccattc caaagcaatt ggctgttttt tggcgtcatt agcttgttga
    12001 tgttagtagt agctgtgtta ggcgcactat tttctgttcg gacaatcgta aaaatcgatc
    12061 cgttgaaggc aattggataa gaaggaggaa gaacatgaaa gcaatagaat ttaaagaagt
    12121 agaaaaaagt ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa caaccatgag
    12181 tatcgataaa ggggaatttg ttgcaatcat cggtccttct ggttcaggta aaagtacttt
    12241 tttgactttg gcaggcggat tgcagacgcc tagcagtggc tctgtattta tcaatgatca
    12301 agaattcagt aatcaaaaag aaagcgaacg cgttaaacta cgttttaaag aaattggctt
    12361 tattttgcaa gcttccaatc tggttccttt tctgacagta aaaaagcagt tattattagt
    12421 agataagatt aaacatgaac atcgtcaaaa agaagcaaac gaattatttg agcagcttgg
    12481 cgtggataag ttgatcaaca agtatccaga agaactatct ggtggagaac ggcaaagggt
    12541 cgcaatcgct cgtgcattgt acaatgatcc gtccattatt ctagcagatg aaccaactgc
    12601 tagtttggat tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga
    12661 caaaaataaa gcaacgatca tggttactca cgatacacgg ctgattgaca aatgcgatag
    12721 aatatttatg atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa
    12781 aaatggtcca tcgatacaac aaagatacac agcaaaaaat tttgaaagaa gcgactgact
    12841 tgtttatgtc caagggatat ctcggcactt caacaagaga aatcgcacaa aaagttggga
    12901 tcacccagcc caatctctat cattatttcg gtgataaaga aaaactatat acggcagttt
    12961 tagaaaacca cttaaaagat gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa
    13021 cgggatttca agtaaccttg acaaaaatgg cgcagttttt gattgagaca catctggtcg
    13081 atttgtttct gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca agagatcatc
    13141 ttttctttct atggaagaaa aattatcgtg aaccttttga agaaatcttt tcagcaaacc
    13201 aatctgtgct aagaaatggg atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt
    13261 tagcacctta catcactcgg tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat
    13321 tgatcgattt atatttgaat ggtgtattag gagaagcgta aagctgttat atagggagaa
    13381 aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgctt ctttatatag
    13441 gtagtttttc ataaatacaa atttgttatt ctctcatgat tatcagggta tatttttatt
    13501 tattatgtcg gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta
    13561 gaatgaagga aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc
    13621 aggatatgcc aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat
    13681 cttttacagc cccttttttg cttacttaag ctgattttaa gttttggccc ttagtctgga
    13741 tctacgaggt gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt
    13801 atcattatta tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg
    13861 gtcagcagta cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa
    13921 gagacattaa tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact
    13981 accccacttg caactataaa cggcggagag ctggcggagt ttctttctga aacaaaaaat
    14041 gcttatcaga tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc
    14101 aaagtcacaa agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta
    14161 gatcctgggc atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa
    14221 aaagatataa cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca
    14281 acggtttatt tgacccatac tacagatgaa ttagtacagc ttggagatat atgcgactac
    14341 agtgaagaaa aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg
    14401 aatgaagcaa ctggaatcac gacatattat tactatggac aagaggaaac attagcacag
    14461 acaattgctg actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac
    14521 tatcaagttc ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat
    14581 aatgatagtg atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt
    14641 ttgacacaag cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag
    14701 ctcctcatat ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt taaacaccgt
    14761 ttaaggaaga ggtcgtgagc ctgccgttca actctgagtc acaaggagca atttcaaaaa
    14821 acagcttctc atgtttgatg actattgtga cttgtgaccg aggagttggc atgagaacac
    14881 cgtttaagaa aaaggttgtg aaatcaacgt tttgttctga gtattagaga aacaaacgga
    14941 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgaggatcag atgattgaac
    15001 accgtttatt cggaacaagg gactgtgaca tgacttttgt cacagttcca ttattttttt
    15061 agtagcttca aagctttttc tgtattggca aaatgaagtt tggcatattt ttcaagcata
    15121 ggcatgccgc ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct
    15181 gacggaaggg tgattccagc ttcagctgat gctttgtcta actcttccaa aaagctggca
    15241 cgactgatga tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag
    15301 tacagttttt cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc
    15361 ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc
    15421 aaatggatcg cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg
    15481 atctcattgt atttttctgg agataagaca agcagtttat aggggatggt tttttttaag
    15541 atgtcagcaa gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt
    15601 ttacgtaatt ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggaccg
    15661 aaatagctgc catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt
    15721 ggcaaagaag aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta
    15781 tcagcttcta ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta
    15841 atggtcgtcc cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa
    15901 taagagcgat agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg
    15961 ttgctcataa aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc
    16021 gttttctaag agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt
    16081 atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa
    16141 ctttcatcaa ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcgg
    16201 tgattgccaa ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg
    16261 taacgaagct gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata
    16321 atgaacaagc agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag
    16381 aaagaatatt ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta
    16441 cagagcttga aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat
    16501 tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt
    16561 taaacgagaa acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca
    16621 aaataagaaa aatttaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga
    16681 ttttattttt attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa
    16741 tcatattcgc gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat
    16801 tagcaagtca tttggaatta tacattccat acccagctgt caccccaaca tcaaagttag
    16861 cattttttga tcaggtattt gcctttcacc tagatgaagc tttttatgca ggaactgctt
    16921 ttctgattat tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg
    16981 gattgacgta tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac
    17041 taatcatggc ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag
    17101 atgtggtcca aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc
    17161 catttcttac caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat
    17221 agtgctggaa aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg
    17281 tatgtccggc acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa
    17341 tccttgaaac attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg
    17401 cacaaggaaa agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa
    17461 actggcttca agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta
    17521 tacctaaaat tgaaaatatc cgtccccata tgaaacggat tgagatcgga gcagacttga
    17581 atggagtgga gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt
    17641 tcattgatga tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc
    17701 agttagtcac gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac
    17761 gcgtgactga tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg
    17821 aacaagctgt tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa
    17881 gtgatgcgat catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc
    17941 gcggtgtatt tggcggtgta gtccacgatc aaagtgcttc aggtcagact ttatttattg
    18001 aaccaaaaca agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa
    18061 atgagattca acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa
    18121 tcatccacaa tacttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg
    18181 gtaaagaagt aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac
    18241 aagcaagaca tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa
    18301 aagactatca agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga
    18361 agactttagg tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag
    18421 aaagtcaaat cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg
    18481 agcaaagtct atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag
    18541 tcaatgaaaa aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag
    18601 gtgctgctct agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg
    18661 caacaacgca ttatcctgaa ttgaaagttt atggatataa tcgggcaaat acgattaatg
    18721 ccagcatgga gtttgatgtg gatacattaa gtccaacgta tcgtttgctg attggcgttc
    18781 ctggacgaag caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg
    18841 atgaagcgaa gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt
    18901 tagagaatcg ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg
    18961 aggcacaaga acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg
    19021 aaaaagaaat ggaaaaagca aagaaaaaag caaatgaagt cgtgtcagag gcggaagaaa
    19081 aagcagaaaa aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg
    19141 tgaaagagca tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa
    19201 ctttgaagaa aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag
    19261 acgaagtgtt ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc
    19321 agtggcaagt agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg
    19381 ttgctcctca aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta
    19441 gtcacgtgcc gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg
    19501 tagatcaata tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg
    19561 gaaaaggaac aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg
    19621 tcaaaagctt tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat
    19681 ttaagtaaag cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata
    19741 ctagtcatag attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac
    19801 atttaatgaa gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg
    19861 tccatgtcgt atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga
    19921 attcagaatt gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat
    19981 catgagcatt ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg
    20041 tgttcattca aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc
    20101 aaaaataagc tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt
    20161 taaggttatt caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt
    20221 ttgacagaca tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac
    20281 gaatcaaaaa caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata
    20341 aaaatggaac gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat
    20401 attttactgg tagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg
    20461 aatacatcgt tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga
    20521 aaaatgatcc taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga
    20581 taacgaatga aaaatatccc cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac
    20641 tgtattttgg accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc
    20701 gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc
    20761 atttgggaca atgcctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa
    20821 tcgttgatga agtgaaaaac tttttaaacg gaggacacga aaaaatcgag tcagagatcc
    20881 atcagaaaat ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag
    20941 accagatccg tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac
    21001 tggatcgaga tgtatttggt tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt
    21061 tcgtaagaca aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag
    21121 cagaagaaga tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc
    21181 ctaaagaagt cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta
    21241 cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga
    21301 atgcctcagt tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga
    21361 tcggagcagt tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt
    21421 cttttgataa ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg
    21481 atggaaaacc ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg
    21541 atgattatgc atcaatgaga gaagtcatct atcgcagata ttcgcgagtg attcgcgaag
    21601 ggttgccttt acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa
    21661 aagatgtctt agaaaatcaa ttaggaatcg atattcctat tgctggcatg gtaaaaaacg
    21721 ataaacataa aacaaatgag ttgctattcg gtacagattt ggctgtagta ccgcttgagc
    21781 gaaattcacc agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa
    21841 tcactttcca tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg
    21901 aagggttagg accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata
    21961 tcacagcagc cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa
    22021 acgtatttga taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg
    22081 tcctatcatt ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg
    22141 cttacgtctg gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag
    22201 aagtcaagga tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc
    22261 aaatccccga tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga
    22321 cttttcgttt gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc
    22381 atcatcttgg ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt
    22441 atccgaaagt aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa
    22501 gaaaagagat tgaaccaaaa atcgactttt ggatggaaaa atttcaggta aaagggaaaa
    22561 agacggataa agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca
    22621 cgttaatcca tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg
    22681 taaatgctga attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca
    22741 ttttttctag ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc
    22801 gaaacggaaa aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta
    22861 cgaagttatt cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga
    22921 aaaaagtgat ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg
    22981 ggaaagaaat ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac
    23041 cgccaacttt ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg
    23101 attattacca aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata
    23161 ttagtccctt ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat
    23221 agtgatgtca ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc
    23281 caagtcaaaa cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa
    23341 gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata
    23401 aatgggatgc tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa
    23461 caactgactg gcttgcaatc gatgcttcga gcgagtcaat taggcatttc accagaagaa
    23521 gtagctagct tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa
    23581 ggaaaaatga cagtcggaga agataactcg acagtccaat acgtcgtaag ttatgtagca
    23641 acgattgtct tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct
    23701 tctgaaaaag gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat
    23761 ttttacggaa aattaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt
    23821 attatttttg gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta
    23881 gacggtatct cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg
    23941 atctttggca ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa
    24001 gcagaagata cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatacatg
    24061 cttggattga tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat
    24121 attccattct tatcttcata tattatgccg attcgcttag caaatgagac agtcggtatg
    24181 ggtggagcat tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg
    24241 tctgcaaaca tgtacaaatc gaatgtactc gtttatagcg aaggcggatt atggagttca
    24301 ttgaaacaat cgatttcaat tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa
    24361 atacagaagg aagctggaca tctattgttc ccagcttctt tttgtataga cctattttat
    24421 ttattttact atattggata tatattcatt gaaaatagtg tataacaact ttataatatt
    24481 gagtatacca atttttggag gcgttttaat ggaccgttat ttagatttac tttcagaaaa
    24541 attcccctca acggaggatg tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct
    24601 tccaaaagga acagagttat ttttgagtga tatacatggc gaatttcctg cttttgatca
    24661 tattctgcgt atcggttcag gaaatcttaa agaaaagatc aaagaccttt tttctgaaca
    24721 aatgacggaa acggatatca gccaattcac tatttttacg gcttatccag aatatgcatt
    24781 aacgactgat tggtacaaag aacaagataa atcgcagctg atccatcatt tgatcgatct
    24841 tttacggttt acgacagtca aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga
    24901 atatagctat atcattgagg aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga
    24961 ctatgtaaaa aaaatcattg aacaacttgt tttgatgaag gaagaagaaa aatttttaac
    25021 gaaacttgca cagacgatcc aaaaatctgt catcgatcat ttgcatatcg ttggagatat
    25081 ttttgaccga ggaactcaag cagccaaagt gatggatcga atcatggatt tttcttctgt
    25141 agatatccaa tggggaaatc atgatatttt gtggattggt gcgtattgcg gctctgaggc
    25201 atgtttactg acacttttac ggattgcagc acgctataat tatttatttg aattagagaa
    25261 agaatatggg ttgaatttac gtccattgtt ttcttttgct catcaaactt atcagaagaa
    25321 tccagtattt gcgcctaaaa acagtaaaaa tctatccgaa agagagcaag aagaattaga
    25381 aaaaatccat caggcacttt ctatccttca attcaaatca gaacatcagt tattggatcg
    25441 tcgacctgaa tttaaaatgg acgatcgtaa attgctggaa aaaatcgact atgaagcatc
    25501 aacgattgat ttagaaggtc agctctatga gttgaaagga acttgttttc aaacgatcca
    25561 gccagatgat ccaaaaaaat tcttggaaga ggaacaaaaa gtgattgata gtttgatgta
    25621 ttcattccag cactctttga gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg
    25681 aatgtatctc acgaacaatc agcatgtgct gtttcatggc tgtatacctg tagacgagaa
    25741 gggacagctc ttagcatttg aattagacca atcttatcgc ggaaaagaac tgctgggctt
    25801 ttttgaaaaa cagataactg aaagcgcaaa agatctgact gcaaaagaag atttagcaac
    25861 cgatttgatt tggtatgcgt ggagtgggcc attttcgcct ttattcggca agagtaaaat
    25921 ggcgactttt gaacgttatt ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc
    25981 ttattatcac ttgcgagatg aagagtggtt cagtgaaaaa attttagcag aattcgacac
    26041 aaaagaagaa gggtcagcaa tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga
    26101 gtcaccgatc aaagcaaatg gaaaagtatt tgtcattgac ggaggtttat ccaaagctta
    26161 tcaaaaaacg acaggaattg ccggttattc actattatgc aattcttatg gtttccaaat
    26221 cgtgacacat tatccgtttt tgcctattga acaacaattt gcaaaaaaat ccgaccaaac
    26281 aaatgtgaaa aaagtaatcg aacagcaact accacgcaaa ctcaatcgtg atacaacaaa
    26341 aggtatggaa ctgcagcagc agattaccca gctcaaaagg ctgttggact atcgaaagga
    26401 cgattgagaa gaattccagt gaacattgtt ccatagactg gagactgaga caaaagtcgt
    26461 gtcgcagtct ctttttccgg ataaacggtg ttcttctgcc aactcctcgg tcacaagtca
    26521 cactattcaa caaacatgaa aagctgtttt ttgaaattgc tccttgtgac tcagagttga
    26581 acggcaggct cacgacctct tggtataaac ggtgttcaaa agccagtttc tcggtattaa
    26641 gccaaattta tgcaaaaatg tgaaaagcca ttttcacaaa atttttctta atactcgaaa
    26701 ctaaacagct ttttcacaac cccctcagaa agtctattaa aattcattca caaagaaatc
    26761 gaataagcgc tgttcagatg gatgtacacg gtgggtaagt tccggatgcc attgaacacc
    26821 gaggatttta tgcatatcat ctttgctttg aaccgcttca acgagtccat cgttagaaaa
    26881 agcgatggct tttaataaag gtgaaagatc tttgactgcc tgatgatggt aagaattcac
    26941 aaaatatgga tcggggagaa ggttgcttaa caaactgtct gaaacaatct tgacttcgtg
    27001 agtcgtaaat tgcggggcag tgggctgttg ttcatgtttt actgaccact ttggatataa
    27061 tgaaagatct tgataaagtg taccttccaa aacgacattc aatagttgga gtccccggca
    27121 gactgcaaaa attggttttc tttgtttcaa tgcttccaag atcagtgcct gttcaaattg
    27181 gtctcgttga atatttgttt ccgttagctt tggatgagga tcttccatgt aaagttgtgg
    27241 actgacatct tgaccacctg caagaataag tttgtcgatt tgtccaatca gttgaggagc
    27301 tgaatctgga gacatgactg gcagaataag aggcaatcct tgagcatcta aaactgcatc
    27361 cacgaatcct tgcggtgtat agctcacttg atttccttgg aaagtatctg ttgctcgaat
    27421 cagttgattt ccggcaatgc cgatgattgg tttcatttgt attcccccta aagtagactt
    27481 attgtaacaa ggatttaaaa aaaattaaac tatgtaagac ttgcccctat acttatctgt
    27541 tcttgctgtg ttactatctg taaagagaaa tttcgtgtga aattcatgac gatagttgaa
    27601 aatattgctt aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct
    27661 tcaaggatct agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac
    27721 taagttatct ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta
    27781 aatcgaaagg aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa
    27841 gaaaatgaaa agcgtgctca aacactgcgg cagcagctga agatctatgc aacagaaaaa
    27901 gagcaggaat tggaaacgat ccaaacaaac tatcgacaac aactaaaaca gttggtggaa
    27961 gaaaaagaac aatttgaaat acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa
    28021 gcagacaagg tgcaagcaac tttgaaagat caatatcaaa agcatcagag tcaagcgatc
    28081 ggtgcgatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac
    28141 cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa
    28201 cgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtgg agaagtattt
    28261 tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggta atcggttgaa
    28321 agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact
    28381 ccatttgaaa cgtcaagaac tttccttaca agatttggaa gccaattatt ccgaagaaaa
    28441 tttcataaat aaattgaaag aagtccttga tttaaaagaa aagtgggaac aactaactga
    28501 agaacgtgat cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat
    28561 tgctccaagt atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc
    28621 aaattttgaa agtttcaagg aagaactgag taagcttgat gacgtttata tggaagaaat
    28681 agattatacg ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgt
    28741 tatagaagaa attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa
    28801 tcctaaagaa aaactgcaag agaccaagca gcaattatcc attgttcacg agcagcaaaa
    28861 gaagataacg acagcgttag gtcagtgcag tggctatatc caagaatttg aatgggtaga
    28921 agaagtcaca cttgccgttg cagaacgaga aaaaataaaa gaacggttta tccatgcgac
    28981 ttatctgatc gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt
    29041 gttaaacgaa accgtatcag aagaagatat atacgtcagt ttcgaaacac caaactcaga
    29101 agagattcag caggaagtcc ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga
    29161 attgctgact gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa caccttggat
    29221 gacaccattt tatcttgtct tcttcgggat gatggtcgca gatgttggtt atggtttgct
    29281 gatgctcatt gggacaatac tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg
    29341 tttcgtaaaa ttctttgaga ttctctcgat accatcgatc atctggggat tgatctacag
    29401 ttcatttttc ggacagtcat taccaaaaga actgtttggc atccgcttgc ctttcccaat
    29461 cctatctacg acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat
    29521 acagattttg gttggattat ttgtggcagc aaaggaaaat atcaggagaa aagattactt
    29581 gagtgctgtc agtgatggat ttgcctggca agggatccta attgggatcg ttattgcgtt
    29641 ggttggtgcc atgttgataa aaaatacagc atttgtttac cttggcgctg ggctatcgat
    29701 cattgctgcc ttatgtatct tgatcgttcc gattatccaa acgccgtcta aagtcaaagg
    29761 tgctgccaaa ggtgcgtata atctttacgg actgaccggc tatatcggtg atttagtcag
    29821 ttatacacgt ttgatggcat tgggtatttc aggcggaagt attggagcgg cattcaatat
    29881 gctagttgct ttcatgccgc cagctgctcg attcagtgta gggatccttt tgatcatcgc
    29941 tttacacgca ctgaatatgt ttttgacact gctaagtgcc tatgtccatg gcgcacgatt
    30001 gcagtatgtc gaattttttg ggaagtttta tacaggaggc ggacgtgcct ttgatccgct
    30061 gaaaacggcg gaaaaatacg tcaacatcaa tcacaagaaa aaaaacaata aataattgga
    30121 ggatttttat ataatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcggt
    30181 attagcgatg gcaacagcga cgattttttc agggatcggt tcagctaaag gtgttggaat
    30241 gacaggtgag gcagcagcag cattgacaac aagtcaaccc gaaaaattcg ggcaggctct
    30301 gattctgcaa ttgcttccag gcacgcaagg tttgtatgga ttcgttattg cctttttgat
    30361 ttttatcaac ttaggaaatg acatgtcagt tgtacaagga ctgaactttc taggtgcttc
    30421 tctgccgatt gcttttacag gcttgttttc aggtattgcc caaggaaaag tcgcagcggc
    30481 aggtattcaa attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc
    30541 gatggttgaa acatacgcga ttttaggttt cgttatttcc ttcctattag tgttgaatgc
    30601 ctaagcataa aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaac
    30661 gaagcagcag agcaggaaag agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa
    30721 aactttgaac aaaaacgtac gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa
    30781 atcgaattac tcgaaaaaaa ataccgtcag ctaaggaatc gccagcaagt agaagtacga
    30841 caagaaaatt tgaatgcgaa gcaggaattt ttaagacgat tgtttgcaga tgcggtaact
    30901 gaaatggaaa attgggatga atctgaacaa atccagttta tcaaaaatgc attgtattca
    30961 ttacctttga caggaaaagt agcattcatt gctggagaaa aatcagcagc ctatctttct
    31021 cagacgttgt tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa
    31081 actgttgctg atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt
    31141 ttcagcagcc ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta
    31201 ttcgaataag gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa
    31261 gagagcttga acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg
    31321 aagcacttgg tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg
    31381 aatatgactt cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg
    31441 aatcagcacc ggagcctgaa atcgtttgga tctatacgat gcgttatact ttccataatc
    31501 tgaaggtttt gacaaaagct gagatgacaa atcaaaatct ggaccatttg tatatcgacg
    31561 atggatttta ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac
    31621 ttcctgaaag cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca
    31681 ttcttcaggg aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg
    31741 ccgataagct aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca
    31801 atatcacaac gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg
    31861 tactttccag ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata
    31921 tggaagatta cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca
    31981 tccgtgaggg aaatattgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt
    32041 cattgtatca agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga
    32101 acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt
    32161 tcgaatcaga aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta
    32221 gttggcgaca aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt
    32281 acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg
    32341 atttatgtca cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa
    32401 caaatgatac ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg
    32461 gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag
    32521 gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct
    32581 gagaacatgt ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc
    32641 ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct
    32701 ggaattggtc caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga
    32761 ccagggatca tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa
    32821 atcacacaaa gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa
    32881 aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc
    32941 gggatcgtag aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa
    33001 ggaacgatca aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt
    33061 gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt
    33121 cggccaatca aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt
    33181 gatacattct tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca
    33241 gggaaaacag tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat
    33301 gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg
    33361 attgacccta atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca
    33421 aacatgccag tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat
    33481 ttccgtgaca tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa
    33541 gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct
    33601 tatcttggtt cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga
    33661 tcagaaggtc gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat
    33721 atttcggaac cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat
    33781 tccagtttag cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt
    33841 tattcaacag aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg
    33901 gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg
    33961 gtagggattg attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt
    34021 cgtgaagatt atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag
    34081 aaacaattca aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg
    34141 tcgttaggtg cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc
    34201 ggacgaagca aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa
    34261 atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata
    34321 tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa
    34381 atatgaagaa ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt
    34441 ggaagtccaa gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt
    34501 acgtgactct tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat
    34561 gatcggtcga gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc
    34621 tgaaaagcat ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga
    34681 tgagtttatc caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca
    34741 aaaattacca gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc
    34801 gcgacaagca acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg
    34861 aatcactttt gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga
    34921 ccgttctgtc atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc
    34981 tcgtatggct ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt
    35041 tatcatgaca gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg
    35101 agaagttcca ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt
    35161 cgaacgagca ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac
    35221 aatgccagaa gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg
    35281 acagatcatc ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact
    35341 cccgtctctt tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca
    35401 tgcagctacg atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt
    35461 agccgtagtt ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc
    35521 tgagcggttt gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga
    35581 aacacttgat ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat
    35641 caaagacgac atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt
    35701 gaatgtgaat ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac
    35761 aagagggcat aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt
    35821 gattcgaaag aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc
    35881 tgattttgta ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt
    35941 gccggcggaa aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc
    36001 tgtgatgaat tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt
    36061 gaattccagt gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact
    36121 tttggaactg acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac
    36181 acgtcggcga gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta
    36241 ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt
    36301 aaaaaatatg ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt
    36361 aaacaaagca aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt
    36421 tttctcctga ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg
    36481 catgggacaa attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga
    36541 ttgacaacat tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt
    36601 ttgattgaga tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata
    36661 aagaagaaag tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa
    36721 gtatcaggtg tgatgaagct catgctttac atcctaaaat tggcctttgt gatccaattg
    36781 attggtacgg ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc
    36841 tggtacagtt tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga
    36901 gacagtttag ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc
    36961 atttctggtg ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag
    37021 aagatgacga ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga
    37081 ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt
    37141 caacgattgg caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca
    37201 gttgattacc tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc
    37261 ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg
    37321 atccagatgc gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc
    37381 cgacaaggcg cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta
    37441 gtcgccatca tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat
    37501 attgcttttg aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca
    37561 gacttgactg caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt
    37621 gtattgactg tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa
    37681 tatccagaag aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt
    37741 agtaaaataa gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt
    37801 tcgtttattt tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg
    37861 aaaggaatca ataagatttg attcctttat ttatcaatag gttataattc atattttttt
    37921 attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg
    37981 gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt
    38041 cggataccgt tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact
    38101 ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca
    38161 aaaagctgat tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca
    38221 tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata
    38281 ttaaaaattg cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact
    38341 cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat
    38401 aaattttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata
    38461 tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt
    38521 ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga
    38581 atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa
    38641 tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta
    38701 tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat
    38761 ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt
    38821 tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta
    38881 agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta
    38941 ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg
    39001 gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc
    39061 gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt
    39121 aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta
    39181 ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat
    39241 ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc
    39301 tccactcctt aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt
    39361 taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct
    39421 tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt
    39481 ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat
    39541 atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt
    39601 accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg
    39661 tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt
    39721 cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca
    39781 cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt
    39841 cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc
    39901 gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat
    39961 gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa
    40021 aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca
    40081 agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct
    40141 gccttaacag aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct
    40201 tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa
    40261 tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa
    40321 tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg
    40381 atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat
    40441 ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat
    40501 ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg
    40561 atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac
    40621 ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag
    40681 attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat
    40741 atgaagaagc gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg
    40801 tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt
    40861 ttgaattagg aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta
    40921 gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta
    40981 atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt
    41041 catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa
    41101 aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa
    41161 taaattggtg gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact
    41221 ttttcaattt aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca
    41281 atcaagagta aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca
    41341 gggtcaatga caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc
    41401 tcaagaccag cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg
    41461 atatgaccat agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct
    41521 aattcatagt catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa
    41581 tcacggcggc ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca
    41641 agatctttca ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt
    41701 ttactcaatc ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa
    41761 tttctcatgt aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa
    41821 ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt
    41881 gtcatagaaa gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt
    41941 cctggcgggg aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata
    42001 gaagaaatgg gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt
    42061 tactcgaatt atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct
    42121 tggaagcgta taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa
    42181 gaagcgcttg tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa
    42241 aaaaataaaa actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta
    42301 cagtacatat tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac
    42361 atttgctgct tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata
    42421 ttcaatcatg gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat
    42481 accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac
    42541 ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa
    42601 acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt
    42661 catgtctggt gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc
    42721 ttcgtggatc gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat
    42781 cgaatggaga tgactgccga cacggcgtgc cattacttcg atctctagga aatcttcatg
    42841 tgtcagtccg tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt
    42901 ataagtaata acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac
    42961 acctggtaag ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc
    43021 aatcgcttct tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat
    43081 gtcgatagcg actagtttga tagacataat ttcctccaat atattttaat ttatttcaat
    43141 caatgcgcca ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata
    43201 atgatcacgc aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg
    43261 ttcttgtccc gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc
    43321 gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc
    43381 ataaggtaga tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt
    43441 ttctactaac aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc
    43501 agatttcagt ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc
    43561 agattcagac cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc
    43621 ttctaaagta aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact
    43681 gctggcatct tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc
    43741 cataccacgg gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat
    43801 ataatcttcc actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg
    43861 aatcacacgt aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc
    43921 atcccgaagc aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac
    43981 ttgtgggttg gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc
    44041 tactcggtta agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat
    44101 ggcttcatga ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac
    44161 atcatgcaac agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt
    44221 gcctattttt tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc
    44281 ataaacgcct aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga
    44341 agtgcctaat tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa
    44401 gattacttga tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat
    44461 aggcagcatt tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt
    44521 gttccattct ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt
    44581 cggtagaatc ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt
    44641 ctttgacttc ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag
    44701 caggatccac tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa
    44761 atcgcttcat gacttgccca cgtttttcct gattaccgtt gacacttaag tacatcataa
    44821 cagaaatccc atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc
    44881 ttaaatcata agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct
    44941 ttggaaaagc gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt
    45001 tttttgtgtc actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga
    45061 tccctaatcc acctgaattt ctgatcactg gagcgtaacc tgcatcttcc agtgtttgga
    45121 tcccgtcact tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat
    45181 attgccagaa atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg
    45241 ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag
    45301 ggttcattca accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa
    45361 tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac
    45421 aaaagaaact aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc
    45481 acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca
    45541 agatttttat tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg
    45601 ttataaagag attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc
    45661 agatggtcat gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta
    45721 taaagaacgg ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt
    45781 tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga
    45841 ttatgatctg ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac
    45901 agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag
    45961 actgtgaaag gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga
    46021 attatcaatg atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt
    46081 ttctgattta gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga
    46141 ttcactcgca caattttata ctgacttgaa tattgacggc agctttatct cattagggga
    46201 taatcgttgg ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca
    46261 tggaatggac gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc
    46321 ctttatcaat gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga
    46381 tctgacagaa gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat
    46441 cgctgcttat aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga
    46501 agacttgcca ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga
    46561 agattatgaa gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga
    46621 gaggttgtga cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca
    46681 cacctt
//
LOCUS       DACCCR010000015        45780 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCCR010000015 DACCCR010000000
VERSION     DACCCR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.015"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            101..544
                     /locus_tag="IVK93_000820"
     CDS             101..544
                     /locus_tag="IVK93_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6386131.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="IVK93_000821"
     CDS             complement(550..1734)
                     /locus_tag="IVK93_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP6386132.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="IVK93_000822"
     CDS             2002..2700
                     /locus_tag="IVK93_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP6386133.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="IVK93_000823"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="IVK93_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP6386134.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="IVK93_000824"
     CDS             complement(3510..4043)
                     /locus_tag="IVK93_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386135.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="IVK93_000825"
     CDS             complement(4340..6721)
                     /locus_tag="IVK93_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6386136.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IVK93_000826"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IVK93_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP6386137.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="IVK93_000827"
     CDS             7422..7961
                     /locus_tag="IVK93_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP6386138.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="IVK93_000828"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="IVK93_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP6386139.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="IVK93_000829"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVK93_000829"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="IVK93_000830"
     CDS             9068..10234
                     /locus_tag="IVK93_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP6386140.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="IVK93_000831"
     CDS             10280..11776
                     /locus_tag="IVK93_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP6386141.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="IVK93_000832"
     CDS             11857..12540
                     /locus_tag="IVK93_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6386142.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13185..14375
                     /locus_tag="IVK93_000833"
     CDS             13185..14375
                     /locus_tag="IVK93_000833"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP6386143.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14477..15430
                     /locus_tag="IVK93_000834"
     CDS             14477..15430
                     /locus_tag="IVK93_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP6386144.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15499..16848
                     /locus_tag="IVK93_000835"
     CDS             15499..16848
                     /locus_tag="IVK93_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6386145.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17031..17354)
                     /locus_tag="IVK93_000836"
     CDS             complement(17031..17354)
                     /locus_tag="IVK93_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386146.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17483..18646
                     /locus_tag="IVK93_000837"
     CDS             17483..18646
                     /locus_tag="IVK93_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386147.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18716..20065
                     /locus_tag="IVK93_000838"
     CDS             18716..20065
                     /locus_tag="IVK93_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6386148.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20110..20541)
                     /locus_tag="IVK93_000839"
     CDS             complement(20110..20541)
                     /locus_tag="IVK93_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6386149.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20929..23571
                     /gene="alaS"
                     /locus_tag="IVK93_000840"
     CDS             20929..23571
                     /gene="alaS"
                     /locus_tag="IVK93_000840"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP6386150.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23771..24367
                     /locus_tag="IVK93_000841"
     CDS             23771..24367
                     /locus_tag="IVK93_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6386151.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24489..25637
                     /locus_tag="IVK93_000842"
     CDS             24489..25637
                     /locus_tag="IVK93_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6386152.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25675..26088)
                     /locus_tag="IVK93_000843"
     CDS             complement(25675..26088)
                     /locus_tag="IVK93_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP6386153.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26167..26598
                     /locus_tag="IVK93_000844"
     CDS             26167..26598
                     /locus_tag="IVK93_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP6386154.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26697..26930)
                     /locus_tag="IVK93_000845"
     CDS             complement(26697..26930)
                     /locus_tag="IVK93_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP6386155.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27113..28783
                     /locus_tag="IVK93_000846"
     CDS             27113..28783
                     /locus_tag="IVK93_000846"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP6386156.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28930..29316
                     /locus_tag="IVK93_000847"
     CDS             28930..29316
                     /locus_tag="IVK93_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386157.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29303..30001
                     /locus_tag="IVK93_000848"
     CDS             29303..30001
                     /locus_tag="IVK93_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6386158.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30006..30470
                     /locus_tag="IVK93_000849"
     CDS             30006..30470
                     /locus_tag="IVK93_000849"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP6386159.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30467..31378
                     /locus_tag="IVK93_000850"
     CDS             30467..31378
                     /locus_tag="IVK93_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6386160.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31613..32152
                     /gene="pyrR"
                     /locus_tag="IVK93_000851"
     CDS             31613..32152
                     /gene="pyrR"
                     /locus_tag="IVK93_000851"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP6386161.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32166..33449
                     /locus_tag="IVK93_000852"
     CDS             32166..33449
                     /locus_tag="IVK93_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP6386162.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33462..34388
                     /locus_tag="IVK93_000853"
     CDS             33462..34388
                     /locus_tag="IVK93_000853"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP6386163.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34407..35687
                     /locus_tag="IVK93_000854"
     CDS             34407..35687
                     /locus_tag="IVK93_000854"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP6386164.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35692..36768
                     /gene="carA"
                     /locus_tag="IVK93_000855"
     CDS             35692..36768
                     /gene="carA"
                     /locus_tag="IVK93_000855"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP6386165.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36773..39958
                     /gene="carB"
                     /locus_tag="IVK93_000856"
     CDS             36773..39958
                     /gene="carB"
                     /locus_tag="IVK93_000856"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP6386166.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40012..40788
                     /locus_tag="IVK93_000857"
     CDS             40012..40788
                     /locus_tag="IVK93_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP6386167.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40785..41717
                     /locus_tag="IVK93_000858"
     CDS             40785..41717
                     /locus_tag="IVK93_000858"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP6386168.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41714..42430
                     /gene="pyrF"
                     /locus_tag="IVK93_000859"
     CDS             41714..42430
                     /gene="pyrF"
                     /locus_tag="IVK93_000859"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP6386169.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42437..43066
                     /locus_tag="IVK93_000860"
     CDS             42437..43066
                     /locus_tag="IVK93_000860"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP6386170.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43054..43368
                     /locus_tag="IVK93_000861"
     CDS             43054..43368
                     /locus_tag="IVK93_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386171.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43406..44047
                     /locus_tag="IVK93_000862"
     CDS             43406..44047
                     /locus_tag="IVK93_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6386172.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44078..44782
                     /locus_tag="IVK93_000863"
     CDS             44078..44782
                     /locus_tag="IVK93_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP6386173.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            44779..45681
                     /locus_tag="IVK93_000864"
     CDS             44779..45681
                     /locus_tag="IVK93_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6386174.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
BASE COUNT        15014 a         8005 c         9886 g        12875 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt tgtcacagtc
    12781 cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa aattaaaaat
    12841 tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa taaataaatt
    12901 aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt gtgttgtatt
    12961 tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc atactatata
    13021 agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa ttctcataat
    13081 cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga cacgatatat
    13141 gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct tccaaagaac
    13201 taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta tacaatacga
    13261 tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa aagatgatca
    13321 atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa aaagcatgta
    13381 ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc aatggttcgc
    13441 tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca gaagcagcaa
    13501 tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta atgacaaaag
    13561 aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt tgccgcttat
    13621 ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag aaaaaagcaa
    13681 aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat ggtgttttct
    13741 ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa aaatatggac
    13801 tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt gcaggaaccg
    13861 tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta tcgaaagcta
    13921 taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg ttgaaagcta
    13981 gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca gctgcattgg
    14041 cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg gaaaatgcta
    14101 attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct gaaacaccta
    14161 ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag aagctgatcg
    14221 aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg acaggcagga
    14281 tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca gtagcagttt
    14341 atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt tcgtctattt
    14401 aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt agtaaaaaag
    14461 atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac aaattggttc
    14521 agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg cgacagatat
    14581 acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg taatggatta
    14641 cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc atttggctgc
    14701 tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga acgtgactgg
    14761 gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta cgccaagttc
    14821 aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata ctcttcagcg
    14881 tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg attattatta
    14941 tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt cttatgcaac
    15001 cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag caatcaaaga
    15061 aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt atatgccaga
    15121 tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg ttcatcgaaa
    15181 tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat ctattcaaaa
    15241 aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt caatagcaga
    15301 atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct tttcgccgaa
    15361 atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa aattggtaga
    15421 gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga ttggctatgg
    15481 tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga agtaaaaata
    15541 gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt tatgggcaca
    15601 gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac aggatccgcc
    15661 agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc aggactaaca
    15721 ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata tatcgaacgg
    15781 atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat ttattttcca
    15841 gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct atttggtctt
    15901 tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct gttgatcaat
    15961 tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg caagcttatt
    16021 cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga cttccaactg
    16081 tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc gggcttgcta
    16141 gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg agaattgaaa
    16201 aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat catgatcgtt
    16261 tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt cgcaggaaac
    16321 agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg aagactggtc
    16381 acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct tactggaatg
    16441 cgtctccctt atgttattgc aaaagaaaac aacctgcctt ttagcaagct ctttgctaaa
    16501 cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt catcgctatt
    16561 ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt tgtcatttgg
    16621 attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga acctgatttg
    16681 tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat cattggaggg
    16741 acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat ggggatagta
    16801 ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct aaaacattcg
    16861 aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat gaggagtacg
    16921 tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt ttgtctacga
    16981 tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt ttactttgat
    17041 ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac caagcaacca
    17101 gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt agacaaagca
    17161 tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag attgaagact
    17221 agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt agttcttttt
    17281 cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc aaaaacaaag
    17341 tacatattca gcatctcgac accccctttg tgtttatctt actttacttt gaaagaaatg
    17401 agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat aagagagata
    17461 caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt ttgcgctgat
    17521 tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg aatatcgaaa
    17581 gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt tttatttatt
    17641 gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg cccaaatgac
    17701 agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag acaacacggt
    17761 attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg tatttttgga
    17821 accagctttt aatgttttat tactcatacc atttggcatt tatttaagat attatttccg
    17881 gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt ttgaattgac
    17941 gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg atgtgaatga
    18001 tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt tatttacatt
    18061 tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa agactgtctc
    18121 attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc ttattaattt
    18181 ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt ctcatatctg
    18241 gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa ccaatggatg
    18301 gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg aacgaatttc
    18361 tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg gtttaagctg
    18421 gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt tgcagatgag
    18481 tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt taaatatctt
    18541 gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc acacatatac
    18601 tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga gcaaaaacgc
    18661 tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg cacgaatgaa
    18721 taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg caaagggctt
    18781 caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag gaaaaaatat
    18841 cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc ctttgtttga
    18901 aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa gtagggaact
    18961 agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac cggagatccg
    19021 tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc tgcatcacca
    19081 acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga atgaacaagc
    19141 gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga cattggatat
    19201 gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc tgcagttttt
    19261 agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat acatggaaaa
    19321 tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg aaaattggct
    19381 gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga caattggtca
    19441 tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa ttgccgatta
    19501 cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc caagagagag
    19561 aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag cgactgattt
    19621 agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag aaattccaga
    19681 agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct taaaaggaac
    19741 ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag aaaaattggg
    19801 cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat acgatcgtaa
    19861 tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga ttggtcttgt
    19921 gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt gggcaattgc
    19981 agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta ctgctcgtaa
    20041 aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc gtgtcacaat
    20101 ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat cttcaataaa
    20161 aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg tcatttcagc
    20221 tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag cttcaagcaa
    20281 ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt ccggcttgaa
    20341 gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt gatcaagaaa
    20401 tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg caggaatccc
    20461 ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta tccattcatc
    20521 cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag atgtattgat
    20581 tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat gaaaataaaa
    20641 attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga aaaggatatg
    20701 atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat agtcactaat
    20761 caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg ttatcgagtt
    20821 taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt tcgtccttgg
    20881 ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat gaaacaatta
    20941 agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg acatacgatt
    21001 gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat caactctggt
    21061 gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc aagaatcaca
    21121 aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac agcacggcac
    21181 catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa aaatgaagcc
    21241 atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga tccagaaaaa
    21301 ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca tgaagaagtt
    21361 ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat cggtgcaggc
    21421 ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa cgatttagca
    21481 gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga aatctggaac
    21541 ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact tcctcataaa
    21601 aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga tgcgtcaacg
    21661 aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct aagcaaacaa
    21721 gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc agatcatatt
    21781 cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg gagagggtat
    21841 gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg tattgaacaa
    21901 gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta ttatcctgaa
    21961 gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga acgtttcaat
    22021 gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt aaaaaacgaa
    22081 aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata tggcttcccg
    22141 gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca tgctggtttt
    22201 gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac tgaaaaatca
    22261 atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt cattggatac
    22321 acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc tcttttgaat
    22381 gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta tgcagaaatg
    22441 ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat cgtagcagtt
    22501 gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga agtaaaagca
    22561 ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg caatcggatc
    22621 atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt acttggcgag
    22681 catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga ctttacccac
    22741 tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa cgaaaaaatc
    22801 tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa aaacatgggt
    22861 gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa tattggcggc
    22921 tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat cggaatcttc
    22981 aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc tgtaacaagt
    23041 aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt agcaacaatt
    23101 gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca acagcaagta
    23161 agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca acaagctggt
    23221 gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc acaagtaaaa
    23281 gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa aaatatttca
    23341 gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc ggcaatgacg
    23401 aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat cgcaccgaaa
    23461 gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa aaatcctgct
    23521 ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata gggcgtttac
    23581 gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg ggcgaagaca
    23641 aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa atggtcggat
    23701 tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat aaataaaaag
    23761 gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg cgatcttgtt
    23821 agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat taggattcat
    23881 tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag gcccaaaaga
    23941 tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct atgccatgcc
    24001 aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag ttcccagagt
    24061 acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa ataaatcaat
    24121 aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag tcttagtgtt
    24181 aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg tagactctgc
    24241 tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag aagcaatagg
    24301 tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac cgttgaaacc
    24361 agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa aggaagagag
    24421 gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa ctagaagggg
    24481 taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa cagcggacaa
    24541 aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg tttgtctcta
    24601 agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca gtgaacagtc
    24661 tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct gcaaagccag
    24721 cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg ttatttgttt
    24781 cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa aaaatcatcc
    24841 atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt tccatctttt
    24901 tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat tctgaaacat
    24961 tgagagctac agcaacagat agttataatg atgtctttac tacactggct gtattgcttt
    25021 cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt tttgctttag
    25081 cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac gaactattag
    25141 gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat gcttgcgatg
    25201 atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa cgctttgcat
    25261 cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag attattgatc
    25321 tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac ttggatccaa
    25381 tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa attcttcttg
    25441 aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga aaaacgctgc
    25501 aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta gaaagaatct
    25561 tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg tttgatcaca
    25621 attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt taatttaaag
    25681 agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat ctgctccttt
    25741 atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata gaagttcttg
    25801 gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt cgaccgtaga
    25861 aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt cgtttttctt
    25921 tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg tccctataga
    25981 aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc cacctccact
    26041 aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa aaagagattc
    26101 tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc ggtgagaggt
    26161 tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat catctattgg
    26221 tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa cacaggtcct
    26281 tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct aggtttgcgg
    26341 cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt ttggaaagac
    26401 cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg ggtgcaaatg
    26461 atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga tcaagtagtc
    26521 ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta tccttatacg
    26581 aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt ttacaggaaa
    26641 agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt gtttatctat
    26701 tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc agcttgtgtt
    26761 cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa agttttgaat
    26821 ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc ataactgata
    26881 aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa agcactcacg
    26941 acctttctaa agtctgatca tagccttcat tataaagagt atttataagt acgtcaaata
    27001 aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata attggcatat
    27061 cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc aaatgaagaa
    27121 tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg ctagtaaatt
    27181 agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa tcaatttttc
    27241 tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta cttccatcaa
    27301 tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag acgcgctcaa
    27361 taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc ctgtgatggg
    27421 aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg aagatatcaa
    27481 cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat tatctgctct
    27541 tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc gagtgatttg
    27601 gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg gcttgggtgg
    27661 gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca gcgaaatcat
    27721 ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg caaggatcgt
    27781 tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag cagaaggcgc
    27841 tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa tttacggaac
    27901 accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca atagtatttt
    27961 agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag gatttggtgc
    28021 agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa aagcgccgga
    28081 tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa tgaaaaaaga
    28141 tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt taaagcgcca
    28201 tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg aattcgtaac
    28261 agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg ggattcttgc
    28321 caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag caaaagctgt
    28381 agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc tagaagaaac
    28441 gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg gtgttgtttt
    28501 cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg acaaactgcc
    28561 gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc taggtgcacc
    28621 ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag gatttttagt
    28681 agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg ctgcattgaa
    28741 tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat tataatctac
    28801 catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg aattaatttt
    28861 gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa agcataatag
    28921 gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat gggtagctcg
    28981 attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca aaagataacg
    29041 cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa aagccccgtt
    29101 tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt gattcttcaa
    29161 atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca aaatttattt
    29221 ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa aaaccgggca
    29281 tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca gtttcaatcg
    29341 aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat ttaccgaact
    29401 tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag atgatctttt
    29461 acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg attttgtcat
    29521 tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag aactgattca
    29581 gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac aagatcttcc
    29641 gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc cgttatacaa
    29701 taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc tagcaaaaga
    29761 aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga tcatccaaaa
    29821 cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg aggttgaagc
    29881 gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg ggaatcccga
    29941 tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag aaaaggattg
    30001 attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg gatcagttgg
    30061 tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt ataccaggta
    30121 ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta gaagggaaga
    30181 tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca ttgatgatta
    30241 aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt ctagctggtg
    30301 cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg ttccagacag
    30361 attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt ggagtgattt
    30421 gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga cagaacttaa
    30481 aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt tattcaccga
    30541 ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg tcaatggaca
    30601 gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct tgatccccga
    30661 accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt acgaagatga
    30721 agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag caggtcatac
    30781 gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta cgatcaacgg
    30841 cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac tgctaatggt
    30901 tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata agacctcttt
    30961 gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa ccatcaatgc
    31021 gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag atgggaagtc
    31081 ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg ttgaattaaa
    31141 actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg gttatccgtt
    31201 agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc agtttctcca
    31261 tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct ttgaagcacc
    31321 tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg cattttaatt
    31381 tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt cctttaagtt
    31441 tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc ataagtagat
    31501 tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta aacattcctc
    31561 ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga aaatgcaagc
    31621 aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta cttatgagat
    31681 tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa cacgaggaat
    31741 ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg atattcctgt
    31801 aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag aagaaccgga
    31861 acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt tgattgatga
    31921 tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg attttggcag
    31981 accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat tgccaatccg
    32041 tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt tagtagagat
    32101 gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt agaggagaga
    32161 gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat atccacgatc
    32221 gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg atgttcggag
    32281 caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta gtcagttcag
    32341 gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg tacttaggaa
    32401 gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt tatccagcga
    32461 ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg atcatcaaaa
    32521 agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt ccagtcatca
    32581 tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat aatcagggca
    32641 cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca attttcttca
    32701 acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt attagcggat
    32761 acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac gctgcctggt
    32821 ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta tatcttggag
    32881 caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc ggccatctta
    32941 tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt cataagacgt
    33001 tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct cctgtaacaa
    33061 gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg tttgtgatcg
    33121 gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc gctttgattt
    33181 taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga gtaattgccg
    33241 caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag aaaaatctgt
    33301 tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa gtaggcacat
    33361 ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat cttatcttac
    33421 cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc aaatcagaac
    33481 gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa gttatgggct
    33541 tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa aagcgccagt
    33601 actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc ttcgatgtag
    33661 cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc tctgtccaaa
    33721 aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta gacgtagcgg
    33781 ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa acaatccagt
    33841 gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc ttgcttgatt
    33901 tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc atcgtaggtg
    33961 atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt ctaggcgcac
    34021 aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg tacggacatt
    34081 atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt cgggtgcaac
    34141 acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag gaacatgggt
    34201 taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac ccagcaccag
    34261 ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg cggatcgttc
    34321 agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg ctaaatggaa
    34381 aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt cagatcaata
    34441 cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga atcaaagcaa
    34501 tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag ggacaattga
    34561 tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt acatacaagg
    34621 agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact gtatgcgcga
    34681 tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta tatgacatca
    34741 ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa aatctgcgaa
    34801 gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct ttcacaaatg
    34861 acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa gctgcaaaaa
    34921 acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc ggcgttatgc
    34981 acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta accgagtctt
    35041 ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac catgtttgcc
    35101 atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca ggaatccacg
    35161 taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt cccgaagatt
    35221 tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa gctttgatcg
    35281 aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc catggattag
    35341 aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc gaaacagcgt
    35401 ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa caagtgatca
    35461 attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg ctaacggttg
    35521 gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc gataaagaag
    35581 actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa ggcgaaacac
    35641 aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag tatggaacgt
    35701 ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc agatgctagt
    35761 gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac tatcactgat
    35821 caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa ttatggggta
    35881 aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt aaaagaacat
    35941 gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt gaaacaaaaa
    36001 ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat ccgtgaagtc
    36061 ggaacgatga aagctagtat cgttgatgct ggagattcct tcgaacatgc atttgatcag
    36121 ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa caaaccttat
    36181 ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa acatagtatt
    36241 ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa cacagatgcc
    36301 gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc tggtgatcca
    36361 aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt accgattttt
    36421 ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac ctataaaatg
    36481 aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg aagaattgat
    36541 tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc agaaaaactc
    36601 atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca cagagattat
    36661 cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga tgcactacat
    36721 ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac agatgccgaa
    36781 acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca ttggtcaagc
    36841 cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag aaggatatga
    36901 agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag tggcagatca
    36961 agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa aagagcgtcc
    37021 agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa tggagctggc
    37081 agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc tttctgcgat
    37141 cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga atcagccaat
    37201 ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa ataaaatcgg
    37261 ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag gtatgtgtga
    37321 aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc ccgctaccca
    37381 atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag tgatgagaga
    37441 ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg tgggtatcca
    37501 tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg aatatcaaat
    37561 gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag gctgtaatgt
    37621 ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca atccgcgtgt
    37681 ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta agctagctgc
    37741 taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag gaacgacttt
    37801 tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct ggccgtttga
    37861 taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg gtgaagtgat
    37921 ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt tagaaatcgg
    37981 tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc taatccaaaa
    38041 agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta gaagagggta
    38101 caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag ataaactgtt
    38161 gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca agatattgaa
    38221 aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg aaataaacga
    38281 agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata agatggttga
    38341 cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct atgaatatga
    38401 aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg gtcctatccg
    38461 tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag caatccagca
    38521 ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga ctgacttttc
    38581 tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga atgtgatcga
    38641 gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa tcaacttagc
    38701 cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag acttggatcg
    38761 tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc ctcaaccttt
    38821 aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa ttggttatcc
    38881 agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg ttgaaaacca
    38941 gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac atccagtact
    39001 agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg acggtgaaac
    39061 agtactcatc ccagggatca tggagcatat tgagcgtgct ggtgtccatt caggggattc
    39121 aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga tcgaagatta
    39181 cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc agtttgtcat
    39241 ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga ctgttccatt
    39301 cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta ttttgggaga
    39361 gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag aagttcatgt
    39421 taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact tagggcctga
    39481 aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag ctttgtacaa
    39541 ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat ttacgattgc
    39601 ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa ttggctatag
    39661 tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg tcgttgaaac
    39721 agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa taagagatgg
    39781 taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt ctaaagacgg
    39841 tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt ctttagacac
    39901 agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc ctatttaacg
    39961 gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca gatgaaacaa
    40021 gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga aatgacattg
    40081 aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat tctcgtgccg
    40141 cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca cgaaacaaat
    40201 acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc tactctttct
    40261 gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt gtccaatctt
    40321 catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc tctatatgag
    40381 ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg ttttgccaca
    40441 catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag aatcgctact
    40501 gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg aacgaagacg
    40561 gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt ggaacagctg
    40621 ttttcttcgc atcaaaatgt ccagttatca ttagaatccc gcatggcatg tgggataggg
    40681 gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa aagtgtgaaa
    40741 gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat gagaatcctt
    40801 tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca agcggctgtt
    40861 tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga tcgatcatgg
    40921 tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg gcggaaacac
    40981 catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt gttatgcata
    41041 aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat gttgctggtg
    41101 cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca aatgtgaaag
    41161 caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct tttggaacag
    41221 attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct gttcctgtct
    41281 atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca atcgaagctg
    41341 gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt gaccttaaga
    41401 caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca atcaagcctg
    41461 tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc attgggatgg
    41521 gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca agtgctgtag
    41581 ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt aaagagctcc
    41641 ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa gaagtgagag
    41701 aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag atttttcaac
    41761 taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat tgtttgtgaa
    41821 aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc taaaagaggc
    41881 tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg tcgaaaaagc
    41941 aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg ctggcggtat
    42001 tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag gtggaaaacg
    42061 tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa tgcatgccga
    42121 tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct gtgccaaaaa
    42181 agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca aagaaatggc
    42241 aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag tcgcaggaga
    42301 tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt ttatcgttat
    42361 aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga ttcaaacaga
    42421 atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat tagaaattga
    42481 agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca aaagcccaat
    42541 ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga tcgcaaaagg
    42601 attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg gaactgcaac
    42661 agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa tggtctatat
    42721 tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta tatttgaagg
    42781 tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct tggaagcagc
    42841 tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct ttacgtatga
    42901 gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga cgttgaccaa
    42961 ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc aagaattaac
    43021 tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc gcttgatgaa
    43081 gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa aatccaaaaa
    43141 ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg tgatcagttt
    43201 ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag aataatctct
    43261 tctctttttt ctattatata tagattttct ctattttttg gtaagggagc tgtttcaaaa
    43321 atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata ttcggaaaaa
    43381 gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag gattgaacaa
    43441 tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata cagttgcagc
    43501 acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag aagcgaatgg
    43561 acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact acacagagcg
    43621 ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa gagggatgct
    43681 gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc cagctgagtt
    43741 ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag aaggagaact
    43801 attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa tcatcaaaaa
    43861 taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga ttaaaagctt
    43921 tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac tattgaacca
    43981 ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa agaaagaaga
    44041 aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata tggatgtcga
    44101 atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg attggtttgc
    44161 gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg aagaaaccaa
    44221 aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg acaaggaatt
    44281 caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt tcaagaaaga
    44341 acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca tcaacggaaa
    44401 acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga atctagttgt
    44461 gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag aaaatgggga
    44521 aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct ttccggagca
    44581 aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg ggccaatcca
    44641 atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc ccttcgatgg
    44701 tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag aaatttatta
    44761 tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc tgtagcaaac
    44821 aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag tctttcagct
    44881 ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac gaatcgaggg
    44941 gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat attggtccag
    45001 cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt ttcgatcgct
    45061 gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga attcaaagac
    45121 gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga agaagtcaaa
    45181 gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc tggtatcaga
    45241 agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa aacgcatatt
    45301 tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga agaattgtta
    45361 tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt ttatgaagac
    45421 cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg tactttgatg
    45481 aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat tggagggatt
    45541 cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt ttatatcatt
    45601 tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga gcttactcgg
    45661 ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga acaccgttga
    45721 tcaaaaaaga ggggttgtga cagaattttg tcacaactcc tctttttgta gcaaacaatt
//
LOCUS       DACCCR010000016        43871 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCCR010000016 DACCCR010000000
VERSION     DACCCR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.016"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(72..791)
                     /locus_tag="IVK93_000865"
     CDS             complement(72..791)
                     /locus_tag="IVK93_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6386175.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(922..2073)
                     /locus_tag="IVK93_000866"
     CDS             complement(922..2073)
                     /locus_tag="IVK93_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP6386176.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2290..3030)
                     /locus_tag="IVK93_000867"
     CDS             complement(2290..3030)
                     /locus_tag="IVK93_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6386177.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3034..3357)
                     /gene="rsfS"
                     /locus_tag="IVK93_000868"
     CDS             complement(3034..3357)
                     /gene="rsfS"
                     /locus_tag="IVK93_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP6386178.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3414..4007)
                     /locus_tag="IVK93_000869"
     CDS             complement(3414..4007)
                     /locus_tag="IVK93_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6386179.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3991..4638)
                     /locus_tag="IVK93_000870"
     CDS             complement(3991..4638)
                     /locus_tag="IVK93_000870"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP6386180.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4651..4962)
                     /gene="yhbY"
                     /locus_tag="IVK93_000871"
     CDS             complement(4651..4962)
                     /gene="yhbY"
                     /locus_tag="IVK93_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP6386181.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4978..6087)
                     /gene="yqeH"
                     /locus_tag="IVK93_000872"
     CDS             complement(4978..6087)
                     /gene="yqeH"
                     /locus_tag="IVK93_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP6386182.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6084..6611)
                     /locus_tag="IVK93_000873"
     CDS             complement(6084..6611)
                     /locus_tag="IVK93_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP6386183.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6791..7576)
                     /locus_tag="IVK93_000874"
     CDS             complement(6791..7576)
                     /locus_tag="IVK93_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP6386184.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7569..8438)
                     /locus_tag="IVK93_000875"
     CDS             complement(7569..8438)
                     /locus_tag="IVK93_000875"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP6386185.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8439..9818)
                     /locus_tag="IVK93_000876"
     CDS             complement(8439..9818)
                     /locus_tag="IVK93_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP6386186.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9826..10245)
                     /gene="fabZ"
                     /locus_tag="IVK93_000877"
     CDS             complement(9826..10245)
                     /gene="fabZ"
                     /locus_tag="IVK93_000877"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6386187.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10270..10746)
                     /gene="accB"
                     /locus_tag="IVK93_000878"
     CDS             complement(10270..10746)
                     /gene="accB"
                     /locus_tag="IVK93_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP6386188.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10749..11984)
                     /gene="fabF"
                     /locus_tag="IVK93_000879"
     CDS             complement(10749..11984)
                     /gene="fabF"
                     /locus_tag="IVK93_000879"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP6386189.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12003..12740)
                     /gene="fabG"
                     /locus_tag="IVK93_000880"
     CDS             complement(12003..12740)
                     /gene="fabG"
                     /locus_tag="IVK93_000880"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP6386190.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12752..13669)
                     /gene="fabD"
                     /locus_tag="IVK93_000881"
     CDS             complement(12752..13669)
                     /gene="fabD"
                     /locus_tag="IVK93_000881"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP6386191.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13776..14000)
                     /locus_tag="IVK93_000882"
     CDS             complement(13776..14000)
                     /locus_tag="IVK93_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6386192.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14053..15015)
                     /locus_tag="IVK93_000883"
     CDS             complement(14053..15015)
                     /locus_tag="IVK93_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP6386193.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15025..15465)
                     /locus_tag="IVK93_000884"
     CDS             complement(15025..15465)
                     /locus_tag="IVK93_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6386194.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            15771..16736
                     /locus_tag="IVK93_000885"
     CDS             15771..16736
                     /locus_tag="IVK93_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6386195.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(16793..17023)
                     /locus_tag="IVK93_000886"
     CDS             complement(16793..17023)
                     /locus_tag="IVK93_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP6386196.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17109..17996)
                     /locus_tag="IVK93_000887"
     CDS             complement(17109..17996)
                     /locus_tag="IVK93_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP6386197.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18085..19308)
                     /locus_tag="IVK93_000888"
     CDS             complement(18085..19308)
                     /locus_tag="IVK93_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6386198.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(19325..20404)
                     /locus_tag="IVK93_000889"
     CDS             complement(19325..20404)
                     /locus_tag="IVK93_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6386199.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(20535..22262)
                     /gene="ptsP"
                     /locus_tag="IVK93_000890"
     CDS             complement(20535..22262)
                     /gene="ptsP"
                     /locus_tag="IVK93_000890"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP6386200.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(22262..22528)
                     /locus_tag="IVK93_000891"
     CDS             complement(22262..22528)
                     /locus_tag="IVK93_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP6386201.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23007..25241
                     /locus_tag="IVK93_000892"
     CDS             23007..25241
                     /locus_tag="IVK93_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6386202.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            25389..25709
                     /locus_tag="IVK93_000893"
     CDS             25389..25709
                     /locus_tag="IVK93_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6386203.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(25914..26138)
                     /locus_tag="IVK93_000894"
     CDS             complement(25914..26138)
                     /locus_tag="IVK93_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP6386204.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(26336..27916)
                     /locus_tag="IVK93_000895"
     CDS             complement(26336..27916)
                     /locus_tag="IVK93_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP6386205.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(28317..29693)
                     /locus_tag="IVK93_000896"
     CDS             complement(28317..29693)
                     /locus_tag="IVK93_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6386206.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(29821..30939)
                     /locus_tag="IVK93_000897"
     CDS             complement(29821..30939)
                     /locus_tag="IVK93_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6386207.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            31081..31515
                     /locus_tag="IVK93_000898"
     CDS             31081..31515
                     /locus_tag="IVK93_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6386208.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(31593..32126)
                     /locus_tag="IVK93_000899"
     CDS             complement(31593..32126)
                     /locus_tag="IVK93_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP6386209.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(32220..33398)
                     /gene="mutY"
                     /locus_tag="IVK93_000900"
     CDS             complement(32220..33398)
                     /gene="mutY"
                     /locus_tag="IVK93_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP6386210.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(33391..34188)
                     /gene="recX"
                     /locus_tag="IVK93_000901"
     CDS             complement(33391..34188)
                     /gene="recX"
                     /locus_tag="IVK93_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP6386211.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            34295..35677
                     /gene="rlmD"
                     /locus_tag="IVK93_000902"
     CDS             34295..35677
                     /gene="rlmD"
                     /locus_tag="IVK93_000902"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6386212.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(35851..36429)
                     /locus_tag="IVK93_000903"
     CDS             complement(35851..36429)
                     /locus_tag="IVK93_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP6386213.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            36751..37221
                     /locus_tag="IVK93_000904"
     CDS             36751..37221
                     /locus_tag="IVK93_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP6386214.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(37329..38555)
                     /locus_tag="IVK93_000905"
     CDS             complement(37329..38555)
                     /locus_tag="IVK93_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP6386215.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(39309..39509)
                     /locus_tag="IVK93_000906"
     CDS             complement(39309..39509)
                     /locus_tag="IVK93_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6386216.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(39643..40251)
                     /locus_tag="IVK93_000907"
     CDS             complement(39643..40251)
                     /locus_tag="IVK93_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP6386217.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(40336..40806)
                     /locus_tag="IVK93_000908"
     CDS             complement(40336..40806)
                     /locus_tag="IVK93_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP6386218.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(40854..41768)
                     /locus_tag="IVK93_000909"
     CDS             complement(40854..41768)
                     /locus_tag="IVK93_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6386219.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            41914..42717
                     /locus_tag="IVK93_000910"
     CDS             41914..42717
                     /locus_tag="IVK93_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP6386220.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            42895..43842
                     /locus_tag="IVK93_000911"
     CDS             42895..43842
                     /locus_tag="IVK93_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6386221.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT        12611 a         9236 c         7429 g        14595 t
ORIGIN      
        1 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
       61 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
      121 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
      181 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
      241 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
      301 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
      361 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
      421 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
      481 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
      541 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
      601 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
      661 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
      721 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
      781 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
      841 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
      901 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
      961 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
     1021 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
     1081 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
     1141 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
     1201 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
     1261 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
     1321 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
     1381 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
     1441 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
     1501 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
     1561 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
     1621 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
     1681 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
     1741 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
     1801 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
     1861 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
     1921 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
     1981 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
     2041 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
     2101 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
     2161 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
     2221 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
     2281 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
     2341 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
     2401 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
     2461 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
     2521 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
     2581 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
     2641 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
     2701 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
     2761 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
     2821 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
     2881 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
     2941 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
     3001 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
     3061 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
     3121 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
     3181 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
     3241 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
     3301 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
     3361 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
     3421 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
     3481 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
     3541 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
     3601 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
     3661 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
     3721 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
     3781 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
     3841 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
     3901 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
     3961 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
     4021 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
     4081 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
     4141 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
     4201 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
     4261 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
     4321 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
     4381 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
     4441 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
     4501 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
     4561 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
     4621 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
     4681 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
     4741 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
     4801 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
     4861 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
     4921 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
     4981 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
     5041 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
     5101 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
     5161 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
     5221 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
     5281 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
     5341 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
     5401 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
     5461 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
     5521 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
     5581 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
     5641 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
     5701 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
     5761 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
     5821 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
     5881 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttgga tctcattata
     5941 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
     6001 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
     6061 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
     6121 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
     6181 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
     6241 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
     6301 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
     6361 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
     6421 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
     6481 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
     6541 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
     6601 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
     6661 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
     6721 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
     6781 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
     6841 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
     6901 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
     6961 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
     7021 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
     7081 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
     7141 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
     7201 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
     7261 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
     7321 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
     7381 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
     7441 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
     7501 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
     7561 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
     7621 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
     7681 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
     7741 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
     7801 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
     7861 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
     7921 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
     7981 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
     8041 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
     8101 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
     8161 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
     8221 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
     8281 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
     8341 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
     8401 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
     8461 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
     8521 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
     8581 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
     8641 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
     8701 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
     8761 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
     8821 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
     8881 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
     8941 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
     9001 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
     9061 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
     9121 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
     9181 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
     9241 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
     9301 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
     9361 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
     9421 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
     9481 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
     9541 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
     9601 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
     9661 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
     9721 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
     9781 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
     9841 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
     9901 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
     9961 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    10021 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    10081 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    10141 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    10201 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    10261 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    10321 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    10381 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    10441 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    10501 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    10561 ttgttcagat atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    10621 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    10681 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    10741 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    10801 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    10861 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    10921 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    10981 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    11041 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    11101 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    11161 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    11221 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    11281 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    11341 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    11401 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    11461 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    11521 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    11581 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    11641 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    11701 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    11761 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    11821 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    11881 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    11941 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    12001 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    12061 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    12121 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    12181 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    12241 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    12301 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    12361 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    12421 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    12481 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    12541 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    12601 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    12661 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    12721 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    12781 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    12841 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    12901 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    12961 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    13021 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    13081 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    13141 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    13201 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    13261 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggta gcttctgtca
    13321 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    13381 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    13441 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    13501 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    13561 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    13621 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    13681 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    13741 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    13801 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    13861 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    13921 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    13981 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    14041 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    14101 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    14161 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    14221 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    14281 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    14341 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    14401 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    14461 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    14521 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    14581 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    14641 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    14701 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    14761 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    14821 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    14881 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    14941 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    15001 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    15061 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    15121 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    15181 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    15241 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    15301 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    15361 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    15421 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    15481 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    15541 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    15601 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    15661 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    15721 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    15781 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    15841 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    15901 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    15961 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    16021 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    16081 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    16141 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    16201 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    16261 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    16321 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    16381 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    16441 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    16501 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    16561 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    16621 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    16681 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    16741 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    16801 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    16861 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    16921 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    16981 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    17041 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    17101 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    17161 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    17221 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    17281 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    17341 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    17401 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    17461 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    17521 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    17581 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    17641 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    17701 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    17761 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    17821 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    17881 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    17941 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    18001 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    18061 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    18121 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    18181 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    18241 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    18301 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    18361 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    18421 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    18481 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    18541 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    18601 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    18661 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    18721 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    18781 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    18841 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    18901 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    18961 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    19021 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    19081 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    19141 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    19201 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    19261 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    19321 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    19381 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    19441 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    19501 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    19561 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    19621 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    19681 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    19741 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    19801 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    19861 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    19921 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    19981 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    20041 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    20101 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    20161 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    20221 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    20281 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    20341 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    20401 acatagccta caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa
    20461 aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc
    20521 ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata
    20581 tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc
    20641 attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata
    20701 cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat
    20761 tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta
    20821 tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga
    20881 atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag
    20941 actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg
    21001 ccttcactta tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct
    21061 ttgagtgtag ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc
    21121 aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat
    21181 gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta
    21241 tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca
    21301 gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg
    21361 aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct
    21421 tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct
    21481 gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca
    21541 aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca
    21601 gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga
    21661 atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca
    21721 aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca
    21781 acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc
    21841 gctaaaatac gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta
    21901 tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca
    21961 gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa
    22021 agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct
    22081 ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct
    22141 tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc
    22201 aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc
    22261 attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt
    22321 cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa
    22381 cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt
    22441 taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg
    22501 ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt
    22561 ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat
    22621 ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa
    22681 agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg
    22741 tttatttcct ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac
    22801 cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag
    22861 agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa
    22921 aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca
    22981 tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa
    23041 cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct
    23101 gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc
    23161 catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc
    23221 aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg
    23281 gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag
    23341 aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag
    23401 tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg
    23461 ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc
    23521 aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg
    23581 gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag
    23641 aaaatgtgat cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg
    23701 atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa
    23761 tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac
    23821 gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga
    23881 aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg
    23941 aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca
    24001 tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta
    24061 aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag
    24121 aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag
    24181 aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg
    24241 ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga
    24301 aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag
    24361 tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct
    24421 ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct
    24481 acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa
    24541 aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg
    24601 gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag
    24661 aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga
    24721 cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg
    24781 ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc
    24841 gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg
    24901 ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc
    24961 tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg
    25021 tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac
    25081 gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta
    25141 tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag
    25201 aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga
    25261 gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt
    25321 cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg
    25381 tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc
    25441 ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct
    25501 acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc
    25561 cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc
    25621 acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac
    25681 atgaattagg cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt
    25741 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca
    25801 gaagcaactc cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga
    25861 attccagctt atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt
    25921 tttgattttt ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct
    25981 ttatttttaa ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg
    26041 tttttgtccc agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc
    26101 tgtttttttc tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta
    26161 atcatatgta cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat
    26221 atagtaaaaa gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg
    26281 tttttgtttg gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca
    26341 tcaaactttt taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt
    26401 tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac
    26461 tcatcttctc atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag
    26521 acatgattac ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta
    26581 gctgaccaac agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg
    26641 ctccttcttg aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt
    26701 ttgcagtcac tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt
    26761 ttagttttcc ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga
    26821 tgatatcgcc tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga
    26881 cattgcttaa tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga
    26941 atgttccaga acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg
    27001 cttggatctt gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat
    27061 cttgtgtttt gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct
    27121 gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa
    27181 ttttttcttg attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt
    27241 gttcatataa ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca
    27301 attctactaa tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt
    27361 agatatcata aagaccttct agccctttac ccataccgat tggccaattc atcggataag
    27421 attcgatatc taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac
    27481 gatctagttt gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata
    27541 atttctttgt ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca
    27601 cggccatcaa agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga
    27661 tatttactcg tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac
    27721 gttgtttctc gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta
    27781 cggtcccggc ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt
    27841 tcccagcatc cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta
    27901 attttggatt attcataacc taactccatt tcttccattc aattatttat attttctcta
    27961 ggcacagatt gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc
    28021 gtgtagcagt ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc
    28081 ccacccaagc caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca
    28141 ggatagcttt ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca
    28201 gcgaatgaaa aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag
    28261 aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat
    28321 cttcttggct cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc
    28381 gcttctgttt cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc
    28441 atttcttcag aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc
    28501 ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc
    28561 agatcactgt caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat
    28621 tcgtgttcat cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct
    28681 ccgacgattt cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca
    28741 tctaataaaa tagccatctg attctgcgtc tttttcattt catataacag atcatcaata
    28801 aagaccgttt caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca
    28861 aagccgaatt tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta
    28921 tctttgtctt cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca
    28981 ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact
    29041 tctcgggcaa ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta
    29101 ttcaatacgc cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct
    29161 tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc
    29221 cagacgaagg gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg
    29281 acttcttctg atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat
    29341 acataggtca aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca
    29401 cctaataccg gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg
    29461 ttgaccaaag tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt
    29521 tttaaaagct tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta
    29581 ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg
    29641 atcaataata aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca
    29701 ttctctcctt ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca
    29761 cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac
    29821 ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt
    29881 gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc
    29941 aagcaatccg gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat
    30001 tttcagtgat ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat
    30061 tagcacaacc acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa
    30121 tgcaggtgct agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat
    30181 gaccccaaat aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt
    30241 accaacgaat aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag
    30301 ttgccccaat aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt
    30361 tggcactagt ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat
    30421 tactacggtt gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact
    30481 aaggaattgc tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat
    30541 atctacttcc ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt
    30601 gatgaaatca ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac
    30661 actaagtaaa atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca
    30721 tattcttttg atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag
    30781 aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa
    30841 aataaagttt atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga
    30901 ccaaaacatt agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct
    30961 tactagtata tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt
    31021 gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc
    31081 atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc
    31141 aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat
    31201 gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta
    31261 cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa
    31321 tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat
    31381 caaggctata atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa
    31441 ctaggatata cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
    31501 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
    31561 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
    31621 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
    31681 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt
    31741 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
    31801 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
    31861 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
    31921 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
    31981 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
    32041 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
    32101 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
    32161 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
    32221 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
    32281 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
    32341 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
    32401 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
    32461 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
    32521 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
    32581 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
    32641 gctccagaat gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt
    32701 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
    32761 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
    32821 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
    32881 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
    32941 actcgcatca cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc
    33001 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
    33061 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
    33121 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
    33181 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
    33241 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
    33301 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
    33361 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
    33421 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
    33481 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
    33541 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg
    33601 gatcacttct tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc
    33661 tttttggctt tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
    33721 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
    33781 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
    33841 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
    33901 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
    33961 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
    34021 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
    34081 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
    34141 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
    34201 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
    34261 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
    34321 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
    34381 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
    34441 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
    34501 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
    34561 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
    34621 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
    34681 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
    34741 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
    34801 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
    34861 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
    34921 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
    34981 aaaaaacgca atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca
    35041 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
    35101 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
    35161 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
    35221 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
    35281 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
    35341 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
    35401 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
    35461 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
    35521 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
    35581 taaaaagttc aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg
    35641 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
    35701 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
    35761 ctgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
    35821 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
    35881 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
    35941 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
    36001 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
    36061 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
    36121 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
    36181 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    36241 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    36301 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    36361 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    36421 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    36481 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    36541 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat
    36601 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    36661 ttatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    36721 aaactaatat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    36781 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    36841 ggaaaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    36901 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    36961 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    37021 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    37081 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg
    37141 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    37201 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    37261 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    37321 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
    37381 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
    37441 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
    37501 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
    37561 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
    37621 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
    37681 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
    37741 aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg
    37801 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
    37861 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
    37921 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
    37981 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
    38041 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
    38101 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
    38161 gccatttcga caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct
    38221 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    38281 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    38341 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    38401 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    38461 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct
    38521 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    38581 aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact
    38641 ttaaaaaaca tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta
    38701 tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat
    38761 ctttttttat gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg
    38821 cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga
    38881 ttatcattcg atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt
    38941 ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc
    39001 attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt
    39061 aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt
    39121 tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc
    39181 ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat
    39241 ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca
    39301 acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc
    39361 gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag
    39421 aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg
    39481 ttgaaccatt taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa
    39541 gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta
    39601 ttttccaaat tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa
    39661 acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg
    39721 gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt
    39781 ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt
    39841 ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc
    39901 ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt
    39961 aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc
    40021 gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa
    40081 taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt
    40141 cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata
    40201 aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt
    40261 cctcctcaat gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt
    40321 cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc
    40381 agtttcttta aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg
    40441 agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc
    40501 ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag
    40561 tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc
    40621 aaacaataca gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata
    40681 ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag
    40741 atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg
    40801 cttcatgagc caacctacct tattaaaaat tcttatttat catactctat atttcaataa
    40861 ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg
    40921 atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc
    40981 ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc
    41041 ttttcgctat actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca
    41101 atttcttgtt ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca
    41161 gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata
    41221 actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt
    41281 accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt caaactttca
    41341 tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct
    41401 actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca
    41461 acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt
    41521 tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt
    41581 gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca
    41641 agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc
    41701 aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct
    41761 tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc
    41821 accaatcatt cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt
    41881 gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt
    41941 tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt
    42001 gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa
    42061 gtcttaaaca acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca
    42121 atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc
    42181 attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg
    42241 atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat
    42301 acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa
    42361 tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca
    42421 ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg
    42481 atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa
    42541 gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt
    42601 gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca
    42661 ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa
    42721 ataaagacaa ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt
    42781 gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc
    42841 aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca
    42901 gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact
    42961 atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag
    43021 atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa
    43081 tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa
    43141 catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta
    43201 ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg
    43261 aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt
    43321 ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc
    43381 tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat
    43441 caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag
    43501 gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc
    43561 atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac
    43621 cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat
    43681 cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat
    43741 tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt tgccacccat
    43801 gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt
    43861 tcaaaagaaa a
//
LOCUS       DACCCR010000017        42668 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCCR010000017 DACCCR010000000
VERSION     DACCCR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.017"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            330..1175
                     /locus_tag="IVK93_000912"
     CDS             330..1175
                     /locus_tag="IVK93_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6386222.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IVK93_000913"
     CDS             1456..2676
                     /locus_tag="IVK93_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP6386223.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IVK93_000914"
     CDS             2673..5393
                     /locus_tag="IVK93_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6386224.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IVK93_000915"
     CDS             5377..6321
                     /locus_tag="IVK93_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6386225.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IVK93_000916"
     CDS             complement(6390..7400)
                     /locus_tag="IVK93_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6386226.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IVK93_000917"
     CDS             complement(7580..7924)
                     /locus_tag="IVK93_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6386227.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IVK93_000918"
     CDS             complement(7917..8351)
                     /locus_tag="IVK93_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP6386228.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IVK93_000919"
     CDS             8489..9226
                     /locus_tag="IVK93_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6386229.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IVK93_000920"
     CDS             9219..10430
                     /locus_tag="IVK93_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6386230.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IVK93_000921"
     CDS             10637..11416
                     /locus_tag="IVK93_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP6386231.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IVK93_000922"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IVK93_000922"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP6386232.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IVK93_000923"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IVK93_000923"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP6386233.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IVK93_000924"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IVK93_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP6386234.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IVK93_000925"
     CDS             15018..16067
                     /locus_tag="IVK93_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP6386235.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IVK93_000926"
     CDS             16064..16342
                     /locus_tag="IVK93_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP6386236.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IVK93_000927"
     CDS             16317..16787
                     /locus_tag="IVK93_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP6386237.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IVK93_000928"
     CDS             16804..17106
                     /locus_tag="IVK93_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386238.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IVK93_000929"
     CDS             17123..17494
                     /locus_tag="IVK93_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP6386239.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IVK93_000930"
     CDS             17466..17825
                     /locus_tag="IVK93_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386240.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IVK93_000931"
     CDS             17997..19004
                     /locus_tag="IVK93_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6386241.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IVK93_000932"
     CDS             19029..20213
                     /locus_tag="IVK93_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP6386242.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDKMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IVK93_000933"
     CDS             complement(20650..22233)
                     /locus_tag="IVK93_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6386243.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IVK93_000934"
     CDS             complement(22811..22954)
                     /locus_tag="IVK93_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP6386244.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="IVK93_000935"
     CDS             complement(23176..23655)
                     /locus_tag="IVK93_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6386245.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="IVK93_000936"
     CDS             complement(23728..24012)
                     /locus_tag="IVK93_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386246.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="IVK93_000937"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVK93_000937"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="IVK93_000938"
     CDS             complement(24356..25642)
                     /locus_tag="IVK93_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP6386247.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="IVK93_000939"
     CDS             25780..26451
                     /locus_tag="IVK93_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP6386248.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="IVK93_000940"
     CDS             26417..26905
                     /locus_tag="IVK93_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP6386249.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="IVK93_000941"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="IVK93_000941"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP6386250.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="IVK93_000942"
     CDS             27866..28630
                     /locus_tag="IVK93_000942"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP6386251.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="IVK93_000943"
     CDS             28863..31076
                     /locus_tag="IVK93_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP6386252.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="IVK93_000944"
     CDS             31087..31533
                     /locus_tag="IVK93_000944"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP6386253.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="IVK93_000945"
     CDS             31704..32963
                     /locus_tag="IVK93_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP6386254.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="IVK93_000946"
     CDS             complement(33052..33999)
                     /locus_tag="IVK93_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6386255.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="IVK93_000947"
     CDS             34393..35685
                     /locus_tag="IVK93_000947"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP6386256.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="IVK93_000948"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="IVK93_000948"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP6386257.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="IVK93_000949"
     CDS             37578..38453
                     /locus_tag="IVK93_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6386258.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="IVK93_000950"
     CDS             38719..40602
                     /locus_tag="IVK93_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP6386259.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="IVK93_000951"
     CDS             complement(40677..40961)
                     /locus_tag="IVK93_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6386260.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="IVK93_000952"
     CDS             complement(40951..41685)
                     /locus_tag="IVK93_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP6386261.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="IVK93_000953"
     CDS             41814..42437
                     /locus_tag="IVK93_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP6386262.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
BASE COUNT        13690 a         7452 c         8862 g        12664 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga taaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaa
//
LOCUS       DACCCR010000018        38503 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCCR010000018 DACCCR010000000
VERSION     DACCCR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.018"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..196)
                     /locus_tag="IVK93_000954"
     rRNA            complement(<1..196)
                     /locus_tag="IVK93_000954"
                     /product="16S ribosomal RNA"
     gene            116..259
                     /locus_tag="IVK93_000955"
     CDS             116..259
                     /locus_tag="IVK93_000955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386263.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(719..2029)
                     /locus_tag="IVK93_000956"
     CDS             complement(719..2029)
                     /locus_tag="IVK93_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6386264.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(2041..3459)
                     /locus_tag="IVK93_000957"
     CDS             complement(2041..3459)
                     /locus_tag="IVK93_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6386265.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3699..5267)
                     /locus_tag="IVK93_000958"
     CDS             complement(3699..5267)
                     /locus_tag="IVK93_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP6386266.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5271..5681)
                     /locus_tag="IVK93_000959"
     CDS             complement(5271..5681)
                     /locus_tag="IVK93_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP6386267.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5678..6601)
                     /locus_tag="IVK93_000960"
     CDS             complement(5678..6601)
                     /locus_tag="IVK93_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP6386268.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6598..7476)
                     /locus_tag="IVK93_000961"
     CDS             complement(6598..7476)
                     /locus_tag="IVK93_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6386269.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7463..8800)
                     /locus_tag="IVK93_000962"
     CDS             complement(7463..8800)
                     /locus_tag="IVK93_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6386270.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8803..9906)
                     /locus_tag="IVK93_000963"
     CDS             complement(8803..9906)
                     /locus_tag="IVK93_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6386271.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9980..11488)
                     /locus_tag="IVK93_000964"
     CDS             complement(9980..11488)
                     /locus_tag="IVK93_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6386272.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11454..12146)
                     /locus_tag="IVK93_000965"
     CDS             complement(11454..12146)
                     /locus_tag="IVK93_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6386273.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(12168..13142)
                     /locus_tag="IVK93_000966"
     CDS             complement(12168..13142)
                     /locus_tag="IVK93_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6386274.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13442..14314
                     /locus_tag="IVK93_000967"
     CDS             13442..14314
                     /locus_tag="IVK93_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6386275.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14772..16970)
                     /locus_tag="IVK93_000968"
     CDS             complement(14772..16970)
                     /locus_tag="IVK93_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6386276.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            17202..18194
                     /locus_tag="IVK93_000969"
     CDS             17202..18194
                     /locus_tag="IVK93_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6386277.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            18254..19675
                     /locus_tag="IVK93_000970"
     CDS             18254..19675
                     /locus_tag="IVK93_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP6386278.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19668..21167
                     /locus_tag="IVK93_000971"
     CDS             19668..21167
                     /locus_tag="IVK93_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP6386279.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            21206..22600
                     /gene="uxaC"
                     /locus_tag="IVK93_000972"
     CDS             21206..22600
                     /gene="uxaC"
                     /locus_tag="IVK93_000972"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP6386280.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22759..23442
                     /locus_tag="IVK93_000973"
     CDS             22759..23442
                     /locus_tag="IVK93_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP6386281.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23444..24280
                     /locus_tag="IVK93_000974"
     CDS             23444..24280
                     /locus_tag="IVK93_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6386282.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24291..26045
                     /locus_tag="IVK93_000975"
     CDS             24291..26045
                     /locus_tag="IVK93_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6386283.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26321..26662)
                     /locus_tag="IVK93_000976"
     CDS             complement(26321..26662)
                     /locus_tag="IVK93_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP6386284.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26730..28901)
                     /locus_tag="IVK93_000977"
     CDS             complement(26730..28901)
                     /locus_tag="IVK93_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6386285.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            29112..29969
                     /locus_tag="IVK93_000978"
     CDS             29112..29969
                     /locus_tag="IVK93_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6386286.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(30040..30897)
                     /locus_tag="IVK93_000979"
     CDS             complement(30040..30897)
                     /locus_tag="IVK93_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6386287.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30882..33584)
                     /locus_tag="IVK93_000980"
     CDS             complement(30882..33584)
                     /locus_tag="IVK93_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6386288.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33597..34886)
                     /locus_tag="IVK93_000981"
     CDS             complement(33597..34886)
                     /locus_tag="IVK93_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP6386289.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            35036..36082
                     /locus_tag="IVK93_000982"
     CDS             35036..36082
                     /locus_tag="IVK93_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6386290.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            36084..38237
                     /locus_tag="IVK93_000983"
     CDS             36084..38237
                     /locus_tag="IVK93_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6386291.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11342 a         7925 c         6861 g        12375 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttatt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcgagag tgtttcccga
      361 ttcatttttg gctgccgtag caacttttat atcatatcag agagacaaac gattgtcaac
      421 tcttttttaa aagttttttt cgatttcgtt tttcgaaatc tttcagcaac tcatttatca
      481 taacttatcg tttgttattt gtcaagaact tttttgaagt ttttttcgat agaatcaagc
      541 ttcgcaagtg gttcttttta acgacttagt tatcttatca cgtctaaaaa catgtgtcaa
      601 ctcttttttg gaattttcat aaaatgtttc aatgaagcgc gacaacgttg tttaatatat
      661 cattaaaaaa aataaaggtc aagctaaaat attatttaag cttgacctta ttataatttc
      721 aacgattgtc aaaagtattg tttgcagctg tgttgccagt ttctatagat aaatcacagc
      781 ttttgaaaca taagttttgt actgcttcga ttcgcccgaa ttcttttgca gttatttggc
      841 aatgagaaaa gcagacatct tctataggct tttcaggaaa tgctactaaa tcaaaagctc
      901 gggaaggaag tgagtaacct ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt
      961 tgactttcgt catttgcttt gattcaggta tttcttcagc cactgcttgc caactttctg
     1021 gcatttgagc aaggtcattg ctattttttc tattgtattc attatgccag tccattatcc
     1081 aagaaaaagg aaattgtaca tcaatcatat tcagattttc taccctgata ttttcgatca
     1141 ctccccctct ttcttttgaa gatttcattc gaataccaca gtctgactga aaaaaatcga
     1201 tatcgtgaat atatacgtca ctaactccag cactaacttc gctaccaata gttacaccgt
     1261 atcctgaacg gattttacat ctagcaacct cgattcttga tgattttcga ttgactcttg
     1321 caccattgcc atctctgcca gacttaatag caatacaatc atcaccacag gccaaatcac
     1381 attcatagac acgcacattt gttgaagaat caatatcaat cccatctgta cttggtccat
     1441 cattgttgcg gatgattact ttttctacta atacatcgtt agaataagtg atttgcaagt
     1501 tccaaaaacc tgatttctga aaaatcaaat cacgaacaac aacatgttct gcatgataca
     1561 gtaaacaagc gcgtggacgt ttaatagcat aatccgcaat ccaacgaagt cccttttgat
     1621 cgtattcagc tcttgtcccg cttttttgat cttttcccca atacagctcc caccaatggt
     1681 ctccttgacc gtcgattatt ccttttcctt ctataagaat atctttcgcc gagagaacat
     1741 tcaaaatcgc tgctggccat ttcatttcta cgccggctac tcgtgtatcg atttctggaa
     1801 atgcttcgat atctgttctc cccttgagga ctgcaccttc ttcaaagata agatgactct
     1861 tatcttttaa aaaaagggct ccgactaaat aaatgccttt tggaataaat acaggtaagt
     1921 gttctttcgc accttgatcg attgcttttt gcagtattat tgtatcatct tgttgagtgt
     1981 cgtttgtttt aaagttcaaa gcattgataa tccctttttt tgtccgcaat acgtttctcc
     2041 ctatctttca ttgatttttt tcaattcttc accaataaat acagtttttg aacgattcag
     2101 tgggtaacct actagcaaaa tgattgcgga tacaaataag cagaatgctg gtaacaagtt
     2161 tgccatcgta taaatccttt cttgtactaa aactgattgg atggctccac ctgttgtaga
     2221 cgattggtaa ccaatgagtg ccagcataaa tccgcctacc gctcctgcac atgcttgtcc
     2281 tacttttctc gcaaaagaat ttactccata tactgttcca tcttctctta agcctgtctt
     2341 atattgatga taatcaatca cgtcagtaat gaatgcccag accatcaaat tgaataaacc
     2401 agatcctaaa gtagcgataa ataaaagaat cagatatacc catgcatttg ttatatgaag
     2461 gaaatacatc agcaagtaca tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt
     2521 tttaccaaat aattttgtca gctttgtagc aaatggtgct aaaactacta ctgttccgta
     2581 agtaaataaa agagcaacag acattgcttg tttattatgg aagtaatcat tgaataaata
     2641 agtcatattc gtcccagcta aaatctgatt gatcacaatg aaaatgtcaa caatgactaa
     2701 aataatcagt gctcgattag ctaacaaacc ttttaccaac tgagaagcag atactttttc
     2761 gtttttgaca actcttactc tttcagtagt caatttacaa gttaacatat atgcgacaaa
     2821 tgctattgca gcacatccga ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg
     2881 tccactcttg ttatgggtat acattaaaat cggaatgaag aatcctgtag ttgatccgcc
     2941 aatcgcagaa ccaatgcttc ggaacgtgga caacgaggtg cggtcattag gatttgagct
     3001 aattgctgct gccattgctc catacgggat attgattgtt gagagaaaaa tcccataaac
     3061 aatgtatgaa acaaaaatat aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg
     3121 caaaaacaaa atgacagtca ctaagcaaaa tggatacttc atttttctga tccaaggaat
     3181 aaatcggcca gtttctgtta actctgagac atcacataat cttccaacca tcacatcagc
     3241 aaacgcatcc aaaattctcg aaatcaagaa taacgtacct acgattgccc ctgaaacgcc
     3301 aagaacattc gtataataaa tcatcaagaa actattgact aaacctaaaa taaaacagtt
     3361 acctagatca ccgaacatgt agcctatctt atcggaaatc ccaaattcct ttttttcttt
     3421 ttttattgta tttgctgtaa cagcattttg tgaaagcatt tgaattcctc ctacctcttc
     3481 tagtaaacgt tttcaaaatt ataactcttt ttttcttgtt tttatattca caatgttggc
     3541 tttttattgc attatcttga cagataaaaa acaaaaaata atctaaaaac attgatgcat
     3601 aagattgata acttttttta cataacaata tatacaaaca aaaaaattca ctaactagtg
     3661 aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc ttttccatca
     3721 tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct
     3781 atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct
     3841 actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt
     3901 acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt
     3961 gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta
     4021 acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta
     4081 aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga
     4141 atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa
     4201 aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc
     4261 cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt
     4321 acctcttcat ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt
     4381 cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata
     4441 gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt
     4501 tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc
     4561 agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact
     4621 gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta
     4681 cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag
     4741 atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat
     4801 ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt
     4861 tcaagatcat ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac
     4921 caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg
     4981 cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt
     5041 ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata
     5101 tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc
     5161 cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct
     5221 ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct
     5281 tttctcattc ttactcgata taaactgaat gccccagctg cgaaataaaa aaatgcaaaa
     5341 ggaaacgcaa taaactgagt gccaatcaac aatatgaatc ctgttataaa atacaaacca
     5401 gtggcagttg ttaagtattt tcctttagca gtcaaaaaca atgctactgc taaaaaagca
     5461 agtgaaataa acaacacaac ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta
     5521 ttttctaaag acaaagttga atgatctaca gcaagcgact gataactttt tttaaactct
     5581 gacgcagaca tattacttaa agtaactgct aacccgccaa agaaaatcaa agatagactg
     5641 ccaccgacca ttcctagcaa tagttcccat tttcttttca tttctgaacc ccctgctttt
     5701 ctaaatgtat ccattcaggt cttttgatcg atgtttcata attgttatta cgttcagaga
     5761 atttgacagt ctgttcattt ttcttgtcat cccaattatc ccaaccagtt gggtgaacat
     5821 gcccacctat cttacacgct tcaaataaga catttgcata cggacgccac ggtcttccta
     5881 aatagacgtt tctagtatca ggttctgcag tcaaggtaca gctttcaaaa cgataccctg
     5941 ttttttgatt tttcggcgta gatgctgctg tgatgtatcc aggattttta ctggctaagc
     6001 tatggatatg acactgatga aaaacggcat ctgccccacc aaatatgaaa tccaccgttc
     6061 cttcaatcca gcaatattca taggtctgac gacaatactc aggattggca gaagccggtg
     6121 tacaaaaagg agtaccatct ttttgcaaat caggcaacgg acccgtgcat aaggtatcct
     6181 gataaccaga tagtcgacag ttttttaaag atacgcgatc tcctgaattg aataaagcaa
     6241 cagcctgtcc taccttttct cctggacctg ctgtattttc gatgtgaaga ttttctaata
     6301 cgacattcga tccttctaag aaaaaagtag cacttcggaa agtccctaac tggctaccgt
     6361 caagatactc ctgttttgca aaacgattac cggagataac tactgttcct gctcccacaa
     6421 gtttaaaatt ggataatctg ctttctatat attcatcata tcttccttct tttactatca
     6481 agacctttgg atggctatcc ggcaggcctg ccaaataatc gattccttct tggatagttt
     6541 caaaatccgt cggggtatct atagcattct ttccaattat cagtttgtct ttcatcctca
     6601 taaatgctcc cttatgaatt gtttccaaat atttctcatt tcttgtgttt ccatatgacc
     6661 gcctgttaaa gaaaaagaag aaaagttttc tggactaccc attgattgat actttttgtc
     6721 cagccctttt ctcagcttca gtacgccttc gtccaagcac atcgtatcgt tttttccaac
     6781 taagctaaga cgagggcgcg gagcgatttt ttcttggatt gctaacgttg tataagctgt
     6841 caatagattt ggcacatagt aataaaaccc atgatgatct aggcggcgat gtagcaaaag
     6901 tatttcgatg tccacctgtg ccgctagatc aatacaaact ttgacacgag aatccaatgc
     6961 tgtcacccac cagcttaata agcctcccat agaaagacca atcgttgcga gccgttgagt
     7021 atctgtgtct tcccttgtct ccaaataatc gatcaagctg atcacatcat aaatacgcat
     7081 tccccataac gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc
     7141 tccgcgttct tcaaatcccc aagcatcaat cgaccaaaca gcatatccca ttcctgtcag
     7201 tacttctgca aaagatggag aagctaagta aggagcacca tggatcaatt cacttttccc
     7261 gactgaaaaa tctccgccgt gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt
     7321 ttctggataa gcatagaggg ctggtactgt ttccaattca tttaagtaca attgatagta
     7381 ttcaagcata aaaccgtttt gtttttctcg gtataatcgt tttccactag gggtatgtct
     7441 gctagatgga gcacctaaaa aatcattcaa ctgcataagc aaagttttta cgaacaacca
     7501 gttcatccat attttctgat tgatttgatt ggaatttctc aatactgatt tctttagtat
     7561 tttctaatag aaatgctggt ccctcgtgat tttcaatcga catctggtta aaggaaacct
     7621 tacccgcaaa gccaatataa aatccttgtc tgctcatttc ctctattcct gtcatcatag
     7681 caggaacacc gggaactgca tcttgggcca tcgagacatc gatattgtca aatgtgattt
     7741 cttgtatgaa ttgttccgct aatccataaa taaaacctgc agaagcatgg acattccttg
     7801 ccgtgatatt cgaaaaatgg atccttctga atgctggtgt ccgttcatct atcggataag
     7861 cctttttttc ccatacatac ggttctttcc ctcgcggacc acagaaataa taaagattca
     7921 gtataaatgg acacatcaca ttatccatga ctatattgga tactcgaata tcttcaacga
     7981 tcccgcctcg acctcgtcta gatttcaatc gtattcctcg atcagtttct tggaagatac
     8041 aatttgaaat ggtaatatta cgaatactac cactcatttc acttcctaat acgacaccgc
     8101 catgtccatg gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt
     8161 acgtatcttc agtccctgcc ttgattgcaa tacagtcatc tcccacgtca atatggcaat
     8221 tactaatacg cacatttttg catgattctg gatcaattcc gtctgtattt ggtgaatccg
     8281 ctgggttcaa gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc gggttgatcg
     8341 tccaacttgg tgactggatc aatttaatat ctttcacagt aattcgatga cagttatgga
     8401 aactcatcaa tttaggacga ggataagcga gattatctgg ttcatttcta aaagtatgcc
     8461 accatttttt tccatttcca tctaatgtac cgaaaccagt gacagagatg ttctctacat
     8521 tttgagcata gatacaggaa gcataaactt ttcgatggac tccttcccaa cgtgaatgaa
     8581 caacaggata atcttttgga tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa
     8641 gttcgacatt gctttttaaa aataaagctc ctgttagaaa ttcacctgct ggaacaacta
     8701 cggtcccgcc tccgtcactt gctgcagcat caatggcttg ttgtattgct tcagtattca
     8761 attcatcaat tgatgcgcca aatgtcaaaa tatcatacat atttataatg cctccacttc
     8821 aatcaatgct tgcaagaatg caccaacgcc tttttgatca ttacaaataa ttggttcact
     8881 gatgtagtaa gcatatgaac catctcgctg atctgctccg cctaaccctg ctacttgaca
     8941 gtttttattc aagttcagcc agccttcttt tgtttccaga acaaattcat caagtaatcc
     9001 ttgatgtgat ttcttcactt gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat
     9061 tcctttagcc attgcgtaaa caaacatact actgccggaa gcttccagat aatttccttt
     9121 ccgttttgtt tctgttgtta cttgatacca tgtcccgctg ttttcttctt gatatggaag
     9181 taatgcagcc agtgtctttt ctagaatttc tttcagcaaa gaacaatctg tttcttcatg
     9241 aagtaattca taggtatctg ctgcagccat caaataccag ccaatcgatc ggctccaaaa
     9301 atgatgggat agacctgttt ttggatcagc ccatggttgg accttctttt catcccatgc
     9361 atgaatcagc aaatttgttt ttggatcgaa tagatgttta taactgattt caaactgtct
     9421 caaaacatct tggtaatctt tttcttcact aaaattctgg ataaattcag cataaaaagg
     9481 agaacccata tataagccat ccaaccaaat ttgataagga taaatttctt tatgccaaaa
     9541 agctccttcg gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt ctgctgcttt
     9601 tttatatttt tcttcgcctg tttctttgta caataaaaag aaaagctttc ctgtatttac
     9661 atggtcgata ttgtattctt caacgctgta gccacggatc gttccatctt cttgcacgaa
     9721 atagtcgata ttgtctttta tgaaatcaaa ataaattttt tcttgggttc gttcccaaag
     9781 taattggaac cctttaagta cgacgccata gtcataagac cattttcctt tataccactt
     9841 ttcttcatat aagcgaggtg ttctttccat gatacttttt gctaatttct ttgaccagtt
     9901 ttccattatt tgctctcctt taaaaattaa aataggattt gggacaaata ccaagaagta
     9961 tttgtcccaa tcgtctatat tactgtgccg ctgctttgta tgcttcctcg tactcttgtg
    10021 tgattcgatc gcctccttgt gctttccagt cttctacagc ttttttgaat ccatcttcgt
    10081 caagctgtcc catgatgtat tgcaccgtag catcaatgac gattttttca atttctgatc
    10141 ctacttcatt gttcgtcggt gaatccaatg gcacagaagg atccattaca gcaaattctt
    10201 cattttcttt gatcaattga ttagagaatt ctttttcagg gtctgtatct ttgaaagaga
    10261 acgtcacttc actaggacga gagcttgata acggttgcac ctcttgttgc caaagatcca
    10321 tatcgataaa ttcgatagaa ccatcatcat tcaattcata atgcgttcct tcgataccgc
    10381 cagtcatcaa tttatacaca tcttcttcta aaagatcatc aacaaattga agtaatttct
    10441 tcaattgtgc ttcgtctttt acttgagatt tgggaaatgc tagcaagccg ccaataccat
    10501 ttccttctga ccaaacatga taaccgccgc cttcaccatc tgagatcttg tttacgggca
    10561 ctaattccat atcatcttga ataccttgac ccatcgtctt cagattcttg atatccacca
    10621 ttccagtgta gattccagtt cttccttgtg caaattgttc ttgctgatca gtcttttggg
    10681 taactgcaaa gtcttgtgcc aaatagccat tctcaaatag atctcttgaa aaattcaaag
    10741 ctttcatata ctcttctgta tcaaattctg gagtgaattt gccattatcg tctacagccc
    10801 agccatttgg tgctccaaaa taagaagtga aaagtttaaa gctggtataa cgaatatcac
    10861 taggcgcacg atcgccaaaa ccaactgtgt cgtccacacc atttccatca ggatcgtttt
    10921 ctgtaaattg tttagctact tcataaagct catccatggt agttggaaca tctagtccta
    10981 gattatccaa ccagtctttt cgaatgacta agccagcacg agcgtagtct ttttgtatcg
    11041 gtacgccgta caaggcaccg tcgatactcg cagcttcgat tctatcttca gaaatttgac
    11101 ttaaattttt atagtctttt aaatattctg atacatccca gaatacgcct gattttaacg
    11161 cattgcggac agaagagttt ttcaacatcg caatttgaag tgtaacgata tctcctagtt
    11221 catcagaagc caatgcagtt gttaatcttt cttcttttga agcatcagga atccagttga
    11281 atgtcagttc cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg
    11341 aagcatgatg caaaatattt aaccaagtga ttttagcttt tccatcttct gtctctgaag
    11401 aactgttact tgaactgctg ccgcatgcgg caagtgctag aattcctgca aaactaagac
    11461 ctaattttac gagcgttgct tttttcataa attttctcct tcactgtttt aataatattt
    11521 ttgttttctt ctgttttttc tgtatatgag ttcttccatt ccaaatggca aaatatagaa
    11581 tttgctgtcc acgtgactgc ggaaaagaag agactgccac cgataaaggt taaaacacct
    11641 ggcattgttt ggatgatcat cagataaatc cctaccatac ttaatatcaa cactaatgag
    11701 taatgaagat gtgagaatcc gaataaaaat gccattttca ttttgaaaaa atatcctttc
    11761 caatcataat gagccatgat tgggaaaata tagagtgctg agagcacata tatgaacgca
    11821 gcaatataca aagcgatcct aagataccaa ttgtgtatga aagaaagatc cacaagtaaa
    11881 acaatcccga tggcgccata tatccagctc atcagcaagg tttcacgata gttttcttta
    11941 aaatatgtga agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag ccattttcta
    12001 atcactgcaa ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg
    12061 actaaaccgc cccacagaaa attcaaataa accaactgta aaatacgatt tagccttccg
    12121 tttattttat ctaatgactg cagcatatct ttccctctct ttctatctta gtaaacgccg
    12181 tcttctccta gctttttggc aatcttatta gctaaaatta ccataaacaa tcctacgacc
    12241 cctttaaaca atcccattgc tgtactgaaa cttaactgtc cattcttcaa tccagctgta
    12301 taaatatagg tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa taacaagtac
    12361 atatgctcga accctaagtc taatgtctga ccgattttta gaatcagcaa ggtaatgatt
    12421 actggacgaa ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc
    12481 atttctgcag cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc
    12541 gtagaccagc ccaactcttt ccagatcact tgtccgatat atactgtgcg tgtccattca
    12601 ggggatgtta gaaaactgat tttttcaaat cccattgttg caaggaattc attcaatacc
    12661 cctccgtcaa cattcaaaaa tacataagta atcgaaacaa tgattaccca agacatgaag
    12721 tgagggatgt aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt tacttcattt
    12781 aatagtaaag acaaaatgat tggcatcgga aaaaacacga agatatttaa tccgaacaag
    12841 atcaacgtat ttcttaataa tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt
    12901 ttgaaaccga cccaaggact tcccattatt cctaaaaatg gttgataatc ttgaaaagca
    12961 ataacgagtc cccccatagg aagataccta aaaatcaaga aataaaggac tcccggcaga
    13021 atcatcacat ataaaaattt attcgttttg attcctgcaa ttattttttt tcgtcttctg
    13081 agcttttctt cttttttcga tagcgtcagt tgaaatggtt cttgtttctt tacactttcc
    13141 ataagcccct ccttagtttt gtgaatgttt gctgcttatg acttcatgat agataactat
    13201 ctgaattacg tatataatca tttttaccgt aagtaaaaac gcttacatag caatgatttt
    13261 tgtgctatac aaaacgataa agtgtatttt ttcacaaata aactacgcat agcaaacctt
    13321 ctattttttt ctatctgaca cttagcatag gaaatgatta tatacgtaat ctttcaccga
    13381 tgataaattg aacatataga aacagaaaca tttaatttat tttgaggagg agcctatcta
    13441 tatgaaaaag aaagtctcta taggagaaag aagttttaca cttttcaaca atacgtttct
    13501 aattttgcta gctttgatct gtatcgttcc ttttttaaat atcatcgcta catcttttgc
    13561 ttctacacaa gaagtcgtcg cgaaaaagtt tatccttttt cctactacct tttctttaga
    13621 cgcgtatcgc tacattcttt ctacgccgac catttttaga gcacttgctg tttcgattgg
    13681 tgttacaggt gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg cctacggtct
    13741 ttccagaaag tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt
    13801 gttcagcggg gggatgattc caacgttctt agttgttcgt tctctagggc tggtcaattc
    13861 ttactgggcc atgatcctgc ctgtggctgt taatgcaatg aatatgatca ttatgagaaa
    13921 ctttttccaa gccttgccgg atagtttgga agaatcagca aaaatggatg gttgcacaga
    13981 ttttggtgta tttttcaaaa ttatgctgcc attagcgtta ccgtctattg ctactatctc
    14041 gcttttttat gctgttactt actggaacac ttatatgact gctattttgt atatcaacga
    14101 ttcatctaaa tggccgatac agattctttt acgccaaatc gtcattgtat caagtgggat
    14161 gcaagcagaa agtagtgccg ttgatgtgat tccaccagca caaacagtta aaatggctgt
    14221 tatcgttatt gcaacagtgc ctatgctgat tgtttatcct tttgttcaga aatattttgt
    14281 caaaggagca ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca aaatggtagt
    14341 tgtgtcagaa gtggtcagct tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa
    14401 tttttgtgaa atttcggctt atttttcgaa gaatctactc ctggaacacc gtgtatcaga
    14461 ggttgtgaaa gagacgttca gccttgagcg atgataaaaa ctctcacaaa cattgcgacg
    14521 agtaacccac aggagcaata agaagcgatt ttgaaaaata gcttcccata tttcacaaaa
    14581 ttatgcctta ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa aaagaactgt
    14641 gacaagactt ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa ctatccataa
    14701 atacaaatac tagggttatt ctaacttctt ttattgcttg aatcagtcca caagaaaaac
    14761 agcctcttga ttcacaaata tttttttcga tattctcccg gcgtaatcga ttccttcttt
    14821 ttaaagaaac gaataaaatt ttgcggattt cgatattgca atctttctgc aatctctttt
    14881 acggagaggt tagtctcttt caaccatttt tttgctacct ctaaacgatg attttgaaca
    14941 aaatcaccaa atttttctcc tgtttccttt ttaaatacat tacttaaata gttagggtta
    15001 taatgcaacc tatcagcgat cacatctaaa gacagatcac gatcatattc tgtgtagacg
    15061 atatgcataa tattctccga caaattttta aattcatgat cagtgattgc ttgagagcat
    15121 tctgtgattg gcagaactaa ttgttctaaa acaagttttt ctagttcttt gggatgataa
    15181 ttgttcagtg cttttacata aatttgtttc atgttatcaa agttttttga atctactccc
    15241 aataactgtc ctaattgaac gagttcattg atcaagcgga ccgaaactac ttcccgactc
    15301 aaaggatttt tattttgtgc aaacaatgca tcaatcaatt catgtactaa attcttcact
    15361 tcctgatttc cagaacgaat ggcttcaaac aatcgatttt gttcttctat cggatatcta
    15421 ataagtgttt tttcatgagc agcaggaacg atttcctgat aaaaaataat tgaacttgga
    15481 ccagtattta cacgataata cagggcttct tttgcccgat caaccgattg cttgctttca
    15541 ttcagcgttt cgaaccggtt actgatgcct atactgatag ttaaattcaa gtattctttt
    15601 acacgttttt gaatgagtcg gcaatactcc atgacttttt tgcttgcttc tgcatcatct
    15661 ttatccattc gataaatagt ggcttgcatt tcttcattca aaacgatcgg cagcattcga
    15721 ttttcctttg gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc
    15781 tccccaccgt gactatcttc taacagatcg atttgaacca aagctgtata atagcagtca
    15841 ttttgagaac tataaccaaa ttgctgcaac cgctgatcta gttctcgcct ttctactcga
    15901 tttctaaata aactaagtac aaatagcgtt tccagctgtg gcttttgggt aactaagtgt
    15961 gcacttaacg cttctttttc tcctatgatc cgactgactg attgagcgac caaatctagt
    16021 tctttcccac tgaatccttt tgatttaaga ttcagacgtt cctgtatttg agaaatcgga
    16081 cgggcaaatc tgtccgaaaa taagtaggaa agaagaccaa tgagtcctac cagtgttaaa
    16141 gacactgcaa ctagcccgat tctcagattg cggatcattg cgccaattgc actagagtca
    16201 acttctaatc tgtaaatcca attattataa tctgatttca gtactatata cgttttatct
    16261 ttttctctta tcactttcag ctgattgtaa tctagatcca cgtccgttaa tacagttggt
    16321 tgctctttat tttttactgt ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa
    16381 agtatgtctt ctccactttc accaacaact tgttctattg tatgtttctc tatcgtagca
    16441 attcccaatg catatttttc tttttcaaac attggcaaag aaatcaccat ttcgattcct
    16501 ttgtttttct tttgccaaaa taaattattt ttgttattta tgtacttctg ttgatacttt
    16561 tcaatttcct cttcggtcag ttgactcaac gatccattga ttactcccca tttaccagcc
    16621 aaactgacta aatcataatt attatcatcc ataacaatcg tttctataaa atttagttct
    16681 ttcctgattt cagaataagt ggagtaatct gtatatgtca gcggatgctt gaatatgtcc
    16741 gagaaatttt tggtcgaacc atacgtagaa aacgaatatt caatcgttct aattttccat
    16801 tcaacgttag ttttaacttg cctgagcaaa ttctttttgt ctgttaaata actcgattcg
    16861 atcgattcat aggtattctg gtaaatgaat aaactaaatc ctatgacaag tacggctcct
    16921 aataaaaaca tcggtaaaaa tattttataa aatactgtac ttttcttcat tttcttcctc
    16981 tcctcctttt aaaataaaag gctttcattt ttctgtaaaa ttgatactca tctaattaaa
    17041 tgagcattcc ccgctttcaa gaatagcata attaaaaata aaaatccaag taatttttaa
    17101 cgtgtgcaat cttttcatat caaagatcta ttgacttatc gtagttatcg taatatgatg
    17161 aatgcgttaa caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg
    17221 tcgctaaagc agctggcgtt tctcatacga cagtttctcg tgctttgaat aacagcagtt
    17281 taataaaaaa tgaaacaaaa caaaaaatac gacaactagc taaggaaatg aattattcgc
    17341 caaactatag tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga ctatttttct
    17401 caagtttgca taaaggaact tcatcaaatt tcctagcaga ttccattgca ggcatccatt
    17461 cagtattaga cacaaattac agtttatctg ttgaaagtat cgatgttgtt gatttaacag
    17521 aaatcaacct gcaacgctac gatggactga tcgttatgag tcaaagtgat gatgatcagc
    17581 ctttcatcga ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca
    17641 atgatccagc ggttatcaac gtcactgccg atgatgcggc cggtgttatg caggcagtag
    17701 attacggtat tggatgcgga catacgaaga tcgcttatat tggcggaaag aaaaattttc
    17761 gttcgacaaa agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg
    17821 tcaatccagc ctatttttta acaggagatt acagcttaga aagcggcctc caaaacatgg
    17881 aatacctatt atcactttct gacctgccta ctctggtttt ttgcggcaac gacgacatag
    17941 cgattggtgc attgagagct gtacatttag ctggcctccg tgttccccag aacatttctc
    18001 tgatcgggtt cgatgattct ccagtcgtct cctatctcaa tccgccgctt accaccgtac
    18061 gtaagccttt gaaagaaatg tgtcaagaag gaacaaagct tttactagat ttgatcgaag
    18121 gcaggacgat cgaacaatct aaaattgagc tctccacctt gttgatcctt cgtgagtcga
    18181 tcaattactt gtaaactaca agtaatttta tataaaaatg ttaacgtgtg cataacacag
    18241 aaaggaagtt tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg aagcacctgt
    18301 aaaagttctt cagtttggtg aaggcaattt tatgcgtgga tttattgatt ggcagattca
    18361 gcagctgaat aaccaacaat tgtttaaagg taatgttgca atcattcaac ctttagaaca
    18421 aggtcttgga aatatgatga accagcaaga taatttatat acggtcattt tacaaggact
    18481 cagcaatggc gaaatcgttg atacttccga gattatcacc gtagtagaac aaatgatcaa
    18541 tccttatgat gaatgggatg cttatctatc tctagcagaa aatgacgact tggcctatat
    18601 cgtctccaat acaacagagg caggaatcca attcgtggag actgataatt taacagatac
    18661 tcccccacag agcctcccgg gcaaactgac agccttttta taccgacgtt tcaaattaaa
    18721 caaagctggc tttaccatca ttccttgcga actgattgac agaaacggcg aacgtctaaa
    18781 agaaatcatt ttacgctacg cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt
    18841 agaaaaagaa aatgttttct gttgtagttt agttgatcgc atcgtcccag gttatcctcg
    18901 cgaagatgca ggtgttttca atcaacggca taactatgaa gatcaattga tggtcaaagc
    18961 agaaccattc atgctttggg tgatcgaagg gcctaaagaa ttggagaatc aactaccatt
    19021 caaacaggct ggcttgaatg tcatcatcac agatgatatg actccttatc gtgaacgtaa
    19081 ggtgcatttg cttaatggac cgcatacagc gatggtccct cttgctttgt tggcggaatt
    19141 ggaaactgta gaagaagtaa tgaaagatcc tttattctcc gcatatgttg atcagttatt
    19201 caatctagaa ttgattccta tgctttcttt acctaaagat gaattagcca tttatgctga
    19261 ccaaatcaaa gaacgtttct tgaatccttt tgcccatcac aaattagaag cgatttcatt
    19321 aaatagtgtt tcaaagttca gcacccgtct gcttccagtg ttcaagaagt atatcgaaga
    19381 acaaaatcag gtaccaccgt taatcactgt ttcactagct gcgctacttc ttatgtatcg
    19441 aggcgatcaa gtaaagccac atgatgatga aaagacgata agcgaattta ctgatgcttg
    19501 gtctgataat ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga
    19561 tttgtctcaa ataccgaatg tgacagatac agttcaagaa atagctgcaa agatcgatca
    19621 aaaaggtgtc cggtcagtat tacaagaaat gaaaggaacg atggtccatg cttgaaaccc
    19681 tgcaaccaac aatgaaatta aatcgacgag acagtgtggt cgtcgcatta acccctattc
    19741 caaaacaaac acaactgacg attgatcagc aaacgatcac cactcttgaa gatatcccgc
    19801 aaggtcataa gattgcattg ggtgatttga aagctggaga caacgtgatc aaatatggct
    19861 atccaatcgg tcatgtgacg acagacgtaa ctgccggtca atggctgcac actcataatg
    19921 taaaaaccaa tctttctggt gaattggatt atcgatatga aaaagatgtc catccaagtc
    19981 attacctttt tgagaaccga acatttcaag gttacttgcg aaaaaatgga aaagtaggga
    20041 ttcgtaatga tttgttttta gtacctaccg ttgggtgtgt gaatggcatt gctgaactga
    20101 tcgtcaaaca gttcaaggaa aaacatccag atctgggaca gtttgaccat attacgattt
    20161 taaagcatcc ttatggctgt tcgcagcttg gaaaagatca tcaaaatact cgagaaattt
    20221 tagcggacgc agtaaatcat cccaatgccg gaggagtttt agtatttgga ttaggttgtg
    20281 agaacaatac agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga
    20341 aatttctagt ggctcaagat gtttacgatg agatcgaaca aggtgtggct ctattagaag
    20401 agcttttaac agcggcggaa aatgaccagc gcatatcagt cccgctctct aagttgaatg
    20461 tcggtttgaa atgcggcggt tctgatggtc tatcgggaat aactgctaat cctttacttg
    20521 gtgcattttc tgactacttg attgctcaag gaggcagcac gatcttaaca gaagtgccag
    20581 aaatgtttgg cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa gtttttgact
    20641 caatcgttga cttgatcaat gacttcaaac agtatttctt gtcttacggg gaaccagttt
    20701 acgaaaaccc atctcctggt aataaagcag gtgggatcac tacattagaa gataagtctt
    20761 taggctgcac gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa
    20821 aaatcagaaa accagggctt agtttattgc aagctcccgg gaatgatctt gtagctgcct
    20881 ctgctcttgc ttcatccgat tgccagttgg tcttgtttac cacaggaaga gggactccgt
    20941 ttggctcata tgtcccaacg gtcaaggtat ctacgaatac aactcttttt gaacgcaaag
    21001 gtcattggat ggattttaat gcaggcgtct tattagaaca accaatggaa gtaatcttag
    21061 aatcgtttgt tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa
    21121 atgacgttcg agaaattgcc atttttaaaa acggagtaac actttgattt tcataaaaca
    21181 gtataaaaag aaagaaggaa gatttatgtt tttatctgat gattttttat taaaagacga
    21241 ttgggcgaaa aagctttacc attcctacgc aaaaaacatg cccattattg attatcactg
    21301 tcacttagat ccaaaagaaa tttacgaaaa caacaatttc agcaatctga cagaagcttg
    21361 gttaggaggc gatcattaca aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa
    21421 aatcacagga aatgcttctg attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa
    21481 aattatcgga aatccgcttt acagttggtc acatcttgaa ttgaagcgtt tcttcggtat
    21541 tgacttgttg ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt
    21601 ggcagctcct gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg
    21661 tacaacagat gatcccgcag acgacttgca ctatcatcag ttgcttgctc atgaagaacc
    21721 agatttgaag gttctacctt cattccgtcc agataaagca ctcaatattg aaaaagaagg
    21781 atttaatgca tgggtcacac tactggaaga agcagctgac caaacgatca ccacctatca
    21841 agaactgata gaagtcttag ggcagcggat agaatacttc catcaacatg gatgtcgtgt
    21901 ttccgaccac gctttagaca tcttacgcta taaagaagcg gatgaaacag tattagaaga
    21961 gatttttcaa aaagcacgag ctaacaaact attatcttca gatgaaatcg atgcttatcg
    22021 tacagaaaca ctgatacatt tgacccactt ctatcattct cataattgga cgatgcagct
    22081 gcacatccat gcttatcgta actgtaatac acaaatgttg gaaaaattag ggcccgatac
    22141 cggctatgat ggaatgaatg atctttcttt gactattcct ctgcaaaaat tattgaaccg
    22201 tgcagaagaa acggatcagt tgccaaagac gatcctttat tcattgaatc cgaatgacta
    22261 tcctgcgatt ttagctttga tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt
    22321 aggttctgga tggtggtaca acgatactcg agctggtatg cgcgatcaat tgacacaatt
    22381 tgctgatggt agtgctttgg ggaattttgt cggcatgctg acagattcaa gaagtttcct
    22441 gtcctacact agacatgaat atttccgtcg tgttctttgt gaatttttag gcgaaatggt
    22501 catgcgagga gaagcacctg aagatgaaca acttttagga agtctagttc aagcgattag
    22561 ttatacaaat gctaaaaatt actttggctt ttttgcctaa ttgattaata gtaacagtaa
    22621 cgaatcaaaa acaggatagt gaaataacgg aatccgttta tccacgtttt agagaagaag
    22681 acggaccaaa tttgctccgt cttcttctct tgctgttctg cttcattgcc tataaacctt
    22741 tatgaaagaa gagataacat gcctactatc catattgccg gtgattctac tgcggcagaa
    22801 aaagatgaaa acaagcgtcc cgagacaggt tggggagaaa aaatctctgc ttatttttca
    22861 cccgagatcc agatcaataa ccaagcaaag aatggacgca gcaccaaatc atttatccag
    22921 gaaggaaggt tagcccgtct agcttcacag tttcagccga atgatttctt atttgtacaa
    22981 tttggacata acgaccagaa aatccaagaa gaaagaggta cgcttccctt cggtagctat
    23041 ttaaaaaatc tgcagatatt ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta
    23101 ctgacatctg tgacgcgacg agattactta gaaaacggaa cattgaatcc agatatactt
    23161 ggagactatc caaaagcgat gcgtgcattt gctgagaaac accatattcc tcttttagat
    23221 gtgttctcaa gatcacaaga attgttccag accttctcaa aagaagagac aaaaaaattc
    23281 tacctgcatc ttatacctga tacttgtaaa aattacccag aaggactaaa agacaatacc
    23341 cactttagtc cagaaggcgc tgataaagtt gcccgaataa ttgttgaatt gataaaagaa
    23401 tcccgtctgc ctcttgctaa ttatttacaa aaaggagttt gagatgtttc cattaaattt
    23461 aggcatccgt gcccacgatt tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc
    23521 acagcttcac ttgtcccata tccaatttgc accacaaaaa gcatttcctg aacaacttaa
    23581 attaactgac atgacacttg ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa
    23641 tatcgaactt tccattttag gatgctatat caacctatcc agcaaaaatc aagaagttcg
    23701 tgcacaagca ttggcaactt tcaaacagca aattgctctg tgctctgcct atcaagccaa
    23761 gatgattgct acggaaaccg gtagtgtctc tgtcggttac acaaaagaaa attttactga
    23821 agaagcttat cagatcgcca gaaattctat cattgaatta gtcgctcatg cagaaaattt
    23881 tggcgtaact gttgctgttg aagcagggat caatcatcca atctataact atcagttagc
    23941 taaacgttta atcgatgaag tagcttcacc aaatttaaaa atcattttag attgtgcgaa
    24001 tttgatgcat aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg ccttggacaa
    24061 tttacatggt tatattacag cgcttcatct caaagactat atcatggaaa atggaaagat
    24121 ccgcatggta cctgttggca agggctgttt agatttccgc ccggtactgc attatatcaa
    24181 atatgagcgt cctctaatgt acgccactct tgaagctact cctgagattt atgttcctga
    24241 agcaatagaa cacttgcagt ttatttacca agattgttaa ggagaagatt atggaggaac
    24301 gaatacagaa aagttatcaa aaatatcatg caaatgcttt tcagacattg ctgcatcttt
    24361 atcagggaaa ttattggcgt tttgccatgt caggactctt ttttattatc aaacatcttc
    24421 catcttggtt aatgccgatc gctattgcca atgtaatcaa tgcagcttca tcgaaagatt
    24481 cttctcagtt gcaggccatt tatttcaacg cttttttgat ggcttgtctg attggtcaaa
    24541 atatattgac gaattatttt catgtaaaat accatagcac ttcgatccgt caagtagaag
    24601 ctggactaag agcgaccctt atcaagaaaa tccaagaact atctattcct tatcaaaaag
    24661 aattgcaatc tggacgaatc cagtcaaaga tcatccgtga tgtagaagct atccaaacgt
    24721 tgtcctctca agtatttgtc agcggattaa atatcgctgt caatttattc gtagctttag
    24781 gtatcacgct ttataaaagt cctgttgttt tcatgttttt cttattgact gttcctctcg
    24841 ccgcattcgt tactctcttt ttccgtaaaa ggatcagaca gtccaactca gacttcaggc
    24901 aagaaatgga agagacttct gcaaaagtga tcgaagtgat cgaattagca ccgattgctc
    24961 gagctcatgg gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt actcaaatct
    25021 atgaaaaagg attccactta gatatcctgc aatctgtttt cggatcagtc agctgggcta
    25081 gttttcagat atttcagctg atttgtttga ttttcactgg gatattagct ttacaagggc
    25141 gcatccaacc cggcgatgtc gtgatgtatc agacgtattt cacgacgatc gtcaattccg
    25201 tttctggaat gatcacgctg attcctacga tttcaaaagg catggaatct atcacttcta
    25261 ttggcgaact gttgattgct ggcgatgtag aaacttatca cggtaagaaa aaaatccaac
    25321 atttggatgg aaattttcgt tttgaggatg tttcttttct ttatccggac tcactcaaac
    25381 cgattatcga tcatttttct ctagaggtga aagctggaga aacaattgct tttgttgggc
    25441 catctggctc tggaaaatct acactcctta acttattgat tggatttatc cagccgactt
    25501 caggaaaaat ttttctagat gatcagcctt tggatgaact ggatatgcga agtgtgcgaa
    25561 attatttagc cgttgttcct caaaccactt tgttgttctc cgcttcgatc aaagagaaca
    25621 tcacttatgg cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc
    25681 aactttcttc actgattgac cagcttcctg atggtctgga taccttagta ggcgaacatg
    25741 gcaacaagct gtctggtggc caaaagcaac gtatctccat tgcacgagcg ttgatacgtc
    25801 agccaaaaat cattttgcta gatgaagcaa cctcagcttt agataaccag tcagagaaaa
    25861 aaatccagca agccctgaat tacctgacag aaacacccac tacttttatt gtggctcatc
    25921 ggctttcaac gatcaaagag gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg
    25981 aaataggtac ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg
    26041 aatgacaaaa aataggagct atgacaagac ttttgtcgta gctcctattt ctgaataaac
    26101 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg
    26161 ttttttgaaa ttgttccttg taactcagag ttaaacggta ggctcacgac ctcttggtaa
    26221 atggtgttca agagacagcc tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg
    26281 aacgtctctt tcacaccctc tgttgtttca taaaatagtt ttattcgctg taaaggtcgc
    26341 gaactggtgt catgatgata cggttcaagt cattgatgat tgtactgaag ccttgctctt
    26401 tttgcatcaa gtcattgatt acttcttctg attggatttt cacagccatt tcttgtgctt
    26461 tcattgcatc ttcgtcgctg aactcttgtc cactcatttg tttttcttgt aattcgattt
    26521 gcatcgcttg gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt actttctcgt
    26581 acgcttcttt caacgctttg aattctggta attcacggat ctcacgttcg atttgattcg
    26641 cgctatcgta gatgtttgac atgttatgcc ctcttttctt ttagtagtta ctctctattg
    26701 taacatgggg ttattcgttt tcaaaggggc tactctcctt gatatttccg ccagaggtct
    26761 ttagccgctt caccaattcc ctgaacgcct tctttcacca tatcgccgaa gtttttcagc
    26821 ccttcttttg cttgttcacc atattgattc aagttatctt gccaatttcc agactctcca
    26881 ttagaatttc ctgtgtttcc aacttgatct gcagctacca cttgtcctcc tgtggcatag
    26941 gcatctgcta cactaaatcc tgtttttgaa gtatacggaa ggatgccttc tgccgctgat
    27001 ttgaagactt tcccgacaac atttccactt gtcccttcca aatagtgcag cttgctggat
    27061 tcttcgtaac ccatccatgt cgaaatcact acgtctggcg tgtaaccgat catccattgg
    27121 tcatttgctt ttgtggcatc gaaattagtt tctgtggttc ctgttttccc tgctatcgta
    27181 taacctgcag gattcgcagc agccgctgtt ccatttgaaa atgtaccaag aagcatgctg
    27241 gtcatttcat cggctgtttc ctttgatatg acctgttttg gttttttgtt tgtgttatcg
    27301 acgatcaccg ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt atacatctga
    27361 ccatcattgg caaatgcact gtaagcaccc gccatgatca atggagatac tcctttttcc
    27421 agtccaccaa gggctaagcc ataatactta tcagattttg ctaaaggcag gccgaattct
    27481 tctgctttat catatccttt ttgtaaaccg atctcatgca aaagccatac agtaggcaaa
    27541 ttcaaacttt gtgcaacagc ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa
    27601 ttatgcgcat cgtaataact ttgcggttcg tctttcaaga tactggaagg cttgtatcca
    27661 gcttccagtg ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc
    27721 atctgtgtag caaagctgaa tcctctaaag acatgatctc ctcttcgtcc tactagtgct
    27781 tgtacaccac ctgtattcgg atctaaagct actgaagcag attggaccat ggcaccatct
    27841 gaagcatttg ctgggaataa tgcactgttt tgataagtag cttctaattg cttttgataa
    27901 tcctgatcta aagctgtata gattttatag cctttattca tgacatcttc ttcatcaaga
    27961 ccatactcgt taactgcttc atcgatcact gcatcaaaat aataaggata acgataacca
    28021 gaatctgaat tatcgtatgt gtcattaaga tacgcgctga tatctgtctg gctttctgct
    28081 tgttcttgtt cttttgtgat ttttccgttt tcttccatta cacttagtac tgtgtttttc
    28141 cgatcattcg cattttttgg gtgatcgatc ggattataaa taccaggacc tttcagcatt
    28201 cctgccaaag tagcagcctc agacagcgta acattcgttg catctactcc aaaatacttt
    28261 ctggaagcgt cttggacacc ccataccccg ttgccaaaat aggcattgtt aagatacatg
    28321 gtcaagattt cttctttgct gtacttcttt tcgatctcga ttgccaaaaa taattccttc
    28381 gcttttcgat caaacgtttg gtctaatgtc agatacgcat ttttggcaag ctgctgagtg
    28441 atcgtacttc ctccgccccc tgatgttccg ccagacaaga ccattcggac tgctgcacgg
    28501 gcaatccctt tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt
    28561 actgcatctt ggatataggg agaaatcgcg tttaattcta catatgttcc tttttgggcg
    28621 tacagtgtgc cagcttcttc gtttttctta tcataaatga ctgtcgattg gcttaatcct
    28681 gattttaacg tttcgacatt tgcttgtttt gctaaaataa agaggtagat acttgtcact
    28741 aagacacaga tcaaaccaat cagcaaaatc aatttgttca catgatattt tttccagatg
    28801 cgctttcgcc atcgatggaa ttgtccgaga tatggtctga tccaacgcca aaaactgatc
    28861 aaagcagctt ttatcttttc taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta
    28921 tttgattcaa tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg cttaagttgt
    28981 agttcaatat gtttttttac gttagtgcaa gtagagtaac ataagaatga acagaaaaat
    29041 gtaagttttt tcttaaatat gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa
    29101 ggagtacact tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa acaacaattc
    29161 gcgacttgct tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt ttgcgtatac
    29221 gtaaaaacgt ttggatcaat caacaacctg ctttgttcca tcatgaagta aagggcaatg
    29281 accagatcac attgttattc gaagaaacag attataatta tcaagaagtt caattaggag
    29341 atgcgaagca catccgtgtt ttatatgaag atgagcattt gattatagtg gataagcctg
    29401 caggaatgaa gactcatcca aatgaaccga cagaaaacag taccttactc aatcatttag
    29461 ctgcttatct cgacactaaa cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa
    29521 caagcggtgc cgttttattt gcaaaaaatc catttgtctt gccgatactt ggaagaatgt
    29581 tggaacaaaa aatgatctat cggagatatc aagccatcgt atggggagaa attgccaaag
    29641 atatgaccat caccaataaa attggacgag atcgccatga tcggagaaaa agggtcgtag
    29701 atgataaaaa tggtcagagc gccttgacac atgtcactgt caacaaagta ctttctgaac
    29761 aaacacaagt ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga gttcatttag
    29821 ctcataaggg acatccgatc gttggagacc ctctctacca aaatagaaca gctgaacgac
    29881 tgatgctcca tgcattagaa ctgcacatga tccatccatt tactcaagaa aaaatagtcg
    29941 gtaaagcttt acctggatta tgggaataga aataaaaaag aacagagtct tatcagcaga
    30001 taatgataag actctgttct ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc
    30061 tgctaacagc aaactaccga taatcccact ttcattcggc agtccccaac gaacgatata
    30121 ttcacttact tgaggtgttt ccatataacc acccatcaat tcaacaaatt gctgacggat
    30181 tttttgaagg agatgatcct gattcattac cccaccgcct aaaataatct tttcaggtgc
    30241 taacgtcaac gtatagttga tcagtgcttg agcaatataa taagcctgaa tagcccaaac
    30301 aggatgatct tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc
    30361 tgctgctaat ccttctaaac aatctttatg ataaggacaa gtaccttcat aatggtcatc
    30421 tggatggcgt ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc
    30481 ttcattgatc acgacacctg caccgattcc tgttcccacg gtaaggtaaa tacaactatt
    30541 ttttccttgt gctgctcctt gcttcaattc cccatatgca gcagcattga catccgttgt
    30601 ccaagcagca ggaatattgt agcgttcctt gattgtgcca agaaaatcga attgtgccca
    30661 cccttttttg ggagttgcca aaacgtatcc gtaattatct tttgttttgt cgataccaat
    30721 cgggccgaat gagccaatcc cgattccctt caattcataa cgatcaaaaa attcaaatac
    30781 ctgatccaat gtttcttctg gtgttgtggt agggatactt aatttttcta aaatttctcc
    30841 accatctgaa accgcacaca caaattttgt tccgcctgct tctattccgc catacattcg
    30901 ttttcctcct cgaatccgat tgtccgaatc tcataaggtt ggattattgg gacaaccgct
    30961 tgcttcgttg gttcttcaag cagattcaat atgacagttt ttccattgtc tttagttatt
    31021 tccaattttt ctaaatgact agacatattg aaacctctta ctactacttt atcactaaac
    31081 ttgctgcgtt tcaaagctgt aattgcaaac gtttcacttt ttatcgttaa atattgttgt
    31141 tttgaaatca atgttccttc atgatgtttg atttgttgag tggaaaaagg aatctgtgct
    31201 gcgtatgcat gcaaataagt agagaatttt tcttctggtc catgtagttc aatgctgtaa
    31261 ttgaaccgat gctcacctag acactgtgct tctggtgttg gaaaatatcc ccaatcgcct
    31321 agttctccca cactgcgaag tagcgtcact gcgatttgtc cgtcttctgt tacttcatat
    31381 tcattcaaac caaagtttcc gactgttaca ccgtactctt catcatgaat attgacaaat
    31441 gcttgctgat gttgcggatt cgtggggttt tcccaacttt tagacacatg gtttgggcgc
    31501 ttcaccactt caaaaatact atctgcttcg tgatgttcca catgtaactt tgtaggaaac
    31561 aagacacgta agcgatgatc tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt
    31621 ttactatctt ttctgattgt catgattgtt tttatttgaa ggattcgttt ttctttggat
    31681 cgttctgctt ttcgataacg aaattccaca accatctgct gttctttttc taaacgctcg
    31741 tctgcagaaa cagggatttc catttcctgt agaatactta tttttgctat ttccggtgta
    31801 tctactaata cttttacttc agagttttct ttgttactgg ataggattgg ctgatcacag
    31861 aaaggttgtt taaagatata ctcatttcct atatcgccga tatcttcaaa aatatgcaga
    31921 ttattcaact ttcgtttgtt tttgcgatct tccattgtaa tggtaccatt tttttcaatc
    31981 gtcaaatgta gagggccgtt ttcgatgatt cgtccactct gatgaatcat tgtttccttt
    32041 tcttctgctt tcgcttcccc ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg
    32101 ttttctaatg gtaaagtcac tttgatcatt cttgccatat atggcacacg gaaacggtct
    32161 ttcggcaaat cataaccaaa ttggacagtt tcttcagata aaatagctgg tatagtggcc
    32221 ccactttttg tttctacatg atacttacgt ttaggcaaat tttctaactc ttgatatagt
    32281 tgttcaggta ttccttcagc gaatcttttt ctttctaatt cgatggttat ttctgcttca
    32341 ccagttttat ccattcctgc agtatttaca atgacaaatg gaaagctgtc ttttgggaat
    32401 ccggtcgtat cgattgctgc ggtcagctgt tctaaggaat cttgtgcaag atactttcca
    32461 acttcatctg ctttttcaaa acgaggtatc atttcccgat ggacagaatc gactgaacaa
    32521 ccgcaaatac tgtcgtgagg atgattttgc attaatgtct tccaagcata atctaattga
    32581 tcatggggat agtttccact aacttcataa gccatcgtcg caagtggttc tgtaatattt
    32641 tctagctgtc gctggacctt ggtattccat tgttttaagt aaacccgagc agatgctgta
    32701 ttggctaaag tataccagcc atccgtttct tgacttgtca attctccttc tactgagcct
    32761 aagtcttctg gcacatcttt ttgaactgct tccagatagt cagtaaaatt agaatgaata
    32821 aactcgtaat ctggaaacag ttcatttgcg agatggattg cttttgatat atctttttgt
    32881 accggttgat gatcaacacc attcatcatc aataagtggt tcgtagaggc atattgttct
    32941 gcatccgcta atttttgctt ccaaaaggca agagctgctt ctttttctac tggaatctca
    33001 ttcccgttgc tataccaatt ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct
    33061 ttccaccaca tctctgagta ttgggaagaa tagttttctg cttccaaaac ttgattatca
    33121 aagccaattg gttttacacc acggccaaac gctgctgctg aaataccagc ttgtttcatt
    33181 aattgaggcg tctgtcccat atttccaaat gtatcgggaa aatagcctaa catgacaggg
    33241 gttccccatt tacggctttc ttccatgcca atcagcatat ttctcacgtt cgattccgag
    33301 ctgataagaa aatcatcttg caagatataa aatggtccaa ttctcaattt tccttcattg
    33361 attgcttgtt ggattgcctg tcttttttct ggccttactt gaagatagtc atctaaaata
    33421 atcgtttgcc catccaaatg aaaactgttg aaggacggat caacttcaaa tagctctagt
    33481 agatcgtcta tcaattcaat cagtcgcatg tgatgctgtt cgtaagccat gtaccattca
    33541 cgatcccagt ggctatgaga aatgatatat actttctttt tcatttacga tacctcctac
    33601 ttttctactc taatatcaaa gtaatccatg actaattcgc aaaacatcat atttgcccaa
    33661 gaaaaccatt ctctcgtata attttcagga ttgttcacat cgaatccttc gtgcataagg
    33721 tgtgttcctg cgtcagttgc tacaagtaaa ttcaatatcc gctctttttc agatttgtct
    33781 gacgttgtca ttccttccat agctaatgca atcggccaaa cgtatttttc tggcgtatgg
    33841 gaggatccaa ttccttttgc gtactttcct tcatagaaat atggattttc tttactcaac
    33901 aatgttcttc ttgtttgcag ataatcttcc tcttgttcag aacaatatcc caagtaaggt
    33961 gcggcgatca gattgggcac attactgtca tccatgactg tggcgtttcc cattccatct
    34021 acttcatatg cataaatcgt ttgccctttt tgattaaccg ttcgtccgtg attctgaatc
    34081 ccctcttgga tctctttctt caatttctgt gtttgctgta caatttcttt atcatcgagt
    34141 atcgtttgaa aaatttcttc cacataacct aacacgacca ccgcaaacat attggatggt
    34201 accagatatc cataacggca agtatcatcg ctaggacgga aaccagacca tgtcattcct
    34261 gtaaaagctg tttttgcacc tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg
    34321 tctcgtacaa atgtataagg agagtgttca tgatcttgct ctgttttgaa cacgtccaag
    34381 atttttttga taccttcaat aaaagattga ttgaattgat cagtttttcc tgtatttttg
    34441 taaagaagat aggccaactg tattgggtag cataaagaat caatttcata ttttctttcc
    34501 cagatccaat cgttcatttt tgtatgatct gtttggtggc cagcaccgtt agcttcttcg
    34561 ttgaaggcat tcgcatatgg atcgatattg atataaaaaa attgcttttt gactaacccg
    34621 ctgatcatgt cagctaattc ctcatcttct tttgcaatca ctaaataagg acgaacttgt
    34681 gcagtagagt ctcttaacca catcgctgga atgtctcccg tcaataaaaa ggtggttccg
    34741 tcattttgct ttttgactgt tgtcaacaga gtattggcaa atgctgctgt aaaattcttt
    34801 gcccagtccg catggatatc cccacatttt tgtgtgatat cttccataaa tttctgcacc
    34861 gattttggta tatctttata agccactaat aaaaccttct ttctatttga tatatcttta
    34921 aagattatac ttcatacagc caattatgtc tacgctttca aaaaatatct ttttatttac
    34981 tttttcatcc attatggtat tatttgaata tgatatatca ttaagaggtg tttatatgaa
    35041 tcaaccattg taccagacaa tatttaatga tttgcaaacg gctattttaa ctggtgaatt
    35101 acctgtagac agccaactac caactgaaaa agaactttca cgtacctata aagtaagcag
    35161 aatcacttcc aaacgtgcat tgactgaatt agagcaatta ggacttattt atcgcgttcg
    35221 cgggaaaggc agtttcgtta aatccgcttc tccggaacag attcttgctt ctcctacaaa
    35281 aacacgacgg attctgtttc ttcttccata tgtcgctgat ttgtctgttg gtgattttag
    35341 caagggattg aaccctgtga tgcaagataa acagattgat gtcatgatga cgacgcttga
    35401 ctttctacaa aataaaacag caaaagagct aatcaatgaa tttgacggat tgatttatta
    35461 tgctcttgat acagaacaac atcttgactt actttttgag ctttcattga aagaattccc
    35521 tgtcgtcata ttagataaaa aaatctatga gttacctttt cctaccgttc tttccgataa
    35581 tttccaaggc ggtgcgcttg ctgcctctta tttgatcgaa cagggacatt caaaaatcgc
    35641 ttatttgttt ggtacgcaga ctcatccaca atccgttcga cagcgttacc ttggttacct
    35701 tgaagcattg gacaaagctg gtttgacttt ccacacttca ctggatgaca aaagcgcgac
    35761 attagatgaa ttgctttctt atatcactaa gtatgaagta actgcttttg tttgtgaaaa
    35821 cgatgttgtc gctatccaga ccatgaatct cttgagacag catggttatc atatccctga
    35881 agatttttca attatcggat ttgatgatat ccaagcagca gctcttgttg acccgccact
    35941 gacaaccatc gcacaagatt ttgaacagtt aggtaagctt gcgggagatg ctttggtcac
    36001 ttggttagag acaaaagatc gaccatccga tagtaaattt ccagtttcat tcatcaaaag
    36061 acaatcaaca aaggagaaat aaaatgaata tcggtcaaat agatactaga catgcaacag
    36121 cgaatcagcc aaatttctct aatgggaact gccagcctta tactggcgtt ccttttggga
    36181 tgaattattt tgcaccgcaa actacggatc aaaacggcag ctggtggttt catcctgatg
    36241 accgcgtttt tcaaggctac cgtcttaccc atcaaccaag tccttggatg ggtgatttca
    36301 gtcatatgct gtttacgccg atcaatggaa aactacagga gaacaccctt ttccatgctc
    36361 aaagttctta ccgcccagaa gaaagtatat tttgcccgac tcatctgtcg atctgtcaat
    36421 tacgttatgg catctgttct acattgatac ctagcatgta tggcggaatc ttatctattg
    36481 attacagcaa aaatgacagc ggtttacttt tatcctttcc gggacgccat cagctctttg
    36541 ttatagatcc ttacacagtg gaaggaaaaa tcacacattt tgctggttca gaagatcctg
    36601 atttttcttt ttattttata ttgagatttg aatatccttt aacaattgat gagttagctg
    36661 tttccaacaa aaatagcgac tcgatcccgt tgtattttga acagacaaaa aaacaaacta
    36721 tccgatttgg gacatctttt atcagctcgg aacaagcagc ttataacctg gaactagaac
    36781 aaaaccaaag caaagagact taccttgctc acagtcaaga acaatggcag tcctattttg
    36841 atcgtataca gctcacacac catgatcaac aacaattgcg gacgttttat cataatttat
    36901 atcgggtgtt tttatttcca caaacatttt atgaaattga tcctagtggg caaaaaatcc
    36961 actatgatac gactagtcga tctataaaac ctggtgttct ctatacaaat aatgggtttt
    37021 gggatacgta taaaacggtt tatcctcttt attctttgat tgctgtagaa aaatacgaag
    37081 aaatgctgga aggttttttg aacagctata gagaaagtgg ctatttgcca aaatggctgt
    37141 ctcctgatga acgtggactt atgcctggga cattgatcga tgcggtcatt gcagatgctg
    37201 ctgtaaaaga tatccgacga gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg
    37261 ctacggttca aagcaaacat ccaaattatg gacgccaagg aacagaagat tacttgacat
    37321 atggctatgt tcctaatcac taccacgaat cagtcaatca cacattagac tatgcttata
    37381 gcgatttttg tattagccaa gttgccaagg tactagaaga agaggaaatg acgaagaaat
    37441 atcagcagca atcattgaac tatcaaaata tctttgacta tgagtcagga tttatgcgtt
    37501 caaaagataa agatggcaag ttcagagaac cattttcagc taatcgttgg ggacaagatt
    37561 atgcagaagg aagtgcttgg caaagcagct ttgctgttta tcacgatttt tcaggactga
    37621 tcggaaaata tggtggcagt gagagatata aagaaaaact cattgagctg tgcaatcagc
    37681 ccccaacgtt tgacccagga ggatatggtt ttgagatcca cgaaatgagt gagatggccg
    37741 cgatagaatt tggacaatta gctatttcaa accagccaag ttttcactat ccttacttgt
    37801 tcagttatat aggacaacct gaaatggcac agccactgct taaacagttg atgactcaga
    37861 ccttcgacga ttctccaatg ggttaccctg gagatgagga caatggaagt atggccgctt
    37921 ggtatatctt caatagtctg ggattttatc ctgtcacgcc tggaaccggt gaatatgtta
    37981 tcggtatgcc attaattgac aaggctgttt tgcttttgtc aaatggaaag gaattgacga
    38041 ttgaagcaac gcctaatcgg ccgcagcaac aatttatcca taaaatcaaa cggatggaca
    38101 aagaatatac gaaattatat tttacccatc aagatttgct gaaaggcggc acgatctctt
    38161 accaattagg gatcatccct caccctaaga aatatcaagc aaaagatttg cctttttcct
    38221 tatcaaacta taattaacaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    38281 agccaagcaa aaatagatcc tcatattttt cgtttggttg gtcttattac cgcagatgca
    38341 agcttttgaa caccgtttat tgagaggttg tgaaatcagc gttctgacct gagtattaga
    38401 gagacaaatg gcaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc
    38461 agctgattga acaccgttta gtggagaggt cgtgaaaaag ctg
//
LOCUS       DACCCR010000019        37873 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCCR010000019 DACCCR010000000
VERSION     DACCCR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.019"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            250..663
                     /locus_tag="IVK93_000984"
     CDS             250..663
                     /locus_tag="IVK93_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP6386292.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(895..1152)
                     /locus_tag="IVK93_000985"
     CDS             complement(895..1152)
                     /locus_tag="IVK93_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386293.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            1315..2289
                     /locus_tag="IVK93_000986"
     CDS             1315..2289
                     /locus_tag="IVK93_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6386294.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            3019..3336
                     /locus_tag="IVK93_000987"
     CDS             3019..3336
                     /locus_tag="IVK93_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386295.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(3540..3905)
                     /locus_tag="IVK93_000988"
     CDS             complement(3540..3905)
                     /locus_tag="IVK93_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386296.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            4113..4595
                     /locus_tag="IVK93_000989"
     CDS             4113..4595
                     /locus_tag="IVK93_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386297.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            4792..5682
                     /gene="pstS"
                     /locus_tag="IVK93_000990"
     CDS             4792..5682
                     /gene="pstS"
                     /locus_tag="IVK93_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP6386298.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            6007..8478
                     /locus_tag="IVK93_000991"
     CDS             6007..8478
                     /locus_tag="IVK93_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6386299.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(8581..10317)
                     /locus_tag="IVK93_000992"
     CDS             complement(8581..10317)
                     /locus_tag="IVK93_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP6386300.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(10314..11018)
                     /locus_tag="IVK93_000993"
     CDS             complement(10314..11018)
                     /locus_tag="IVK93_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6386301.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            11149..11652
                     /locus_tag="IVK93_000994"
     CDS             11149..11652
                     /locus_tag="IVK93_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP6386302.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            11612..11950
                     /locus_tag="IVK93_000995"
     CDS             11612..11950
                     /locus_tag="IVK93_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP6386303.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            11971..13389
                     /gene="ffh"
                     /locus_tag="IVK93_000996"
     CDS             11971..13389
                     /gene="ffh"
                     /locus_tag="IVK93_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP6386304.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            13501..14079
                     /locus_tag="IVK93_000997"
     CDS             13501..14079
                     /locus_tag="IVK93_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP6386305.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            14083..14604
                     /locus_tag="IVK93_000998"
     CDS             14083..14604
                     /locus_tag="IVK93_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6386306.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(14683..15237)
                     /locus_tag="IVK93_000999"
     CDS             complement(14683..15237)
                     /locus_tag="IVK93_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6386307.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            15490..15765
                     /gene="rpsP"
                     /locus_tag="IVK93_001000"
     CDS             15490..15765
                     /gene="rpsP"
                     /locus_tag="IVK93_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP6386308.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            15777..16028
                     /locus_tag="IVK93_001001"
     CDS             15777..16028
                     /locus_tag="IVK93_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6386309.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            16153..16944
                     /locus_tag="IVK93_001002"
     CDS             16153..16944
                     /locus_tag="IVK93_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386310.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            17114..17635
                     /gene="rimM"
                     /locus_tag="IVK93_001003"
     CDS             17114..17635
                     /gene="rimM"
                     /locus_tag="IVK93_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP6386311.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            17625..18386
                     /gene="trmD"
                     /locus_tag="IVK93_001004"
     CDS             17625..18386
                     /gene="trmD"
                     /locus_tag="IVK93_001004"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP6386312.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            18522..18869
                     /gene="rplS"
                     /locus_tag="IVK93_001005"
     CDS             18522..18869
                     /gene="rplS"
                     /locus_tag="IVK93_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP6386313.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            19242..19904
                     /locus_tag="IVK93_001006"
     CDS             19242..19904
                     /locus_tag="IVK93_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386314.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            19983..23210
                     /gene="fss3"
                     /locus_tag="IVK93_001007"
     CDS             19983..23210
                     /gene="fss3"
                     /locus_tag="IVK93_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP6386315.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            23324..23638
                     /locus_tag="IVK93_001008"
     CDS             23324..23638
                     /locus_tag="IVK93_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6386316.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            23651..24025
                     /locus_tag="IVK93_001009"
     CDS             23651..24025
                     /locus_tag="IVK93_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6386317.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            24035..24379
                     /locus_tag="IVK93_001010"
     CDS             24035..24379
                     /locus_tag="IVK93_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP6386318.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            24450..25802
                     /locus_tag="IVK93_001011"
     CDS             24450..25802
                     /locus_tag="IVK93_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6386319.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            25862..27004
                     /locus_tag="IVK93_001012"
     CDS             25862..27004
                     /locus_tag="IVK93_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP6386320.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            27029..27283
                     /locus_tag="IVK93_001013"
     CDS             27029..27283
                     /locus_tag="IVK93_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386321.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            27539..28723
                     /locus_tag="IVK93_001014"
     CDS             27539..28723
                     /locus_tag="IVK93_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6386322.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            28738..28857
                     /locus_tag="IVK93_001015"
     CDS             28738..28857
                     /locus_tag="IVK93_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP6386323.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            28862..29083
                     /locus_tag="IVK93_001016"
     CDS             28862..29083
                     /locus_tag="IVK93_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386324.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            29096..29596
                     /locus_tag="IVK93_001017"
     CDS             29096..29596
                     /locus_tag="IVK93_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6386325.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            29693..31540
                     /locus_tag="IVK93_001018"
     CDS             29693..31540
                     /locus_tag="IVK93_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP6386326.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            31543..32439
                     /locus_tag="IVK93_001019"
     CDS             31543..32439
                     /locus_tag="IVK93_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6386327.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            32489..32878
                     /locus_tag="IVK93_001020"
     CDS             32489..32878
                     /locus_tag="IVK93_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6386328.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            32865..34367
                     /locus_tag="IVK93_001021"
                     /pseudo
     CDS             32865..34367
                     /locus_tag="IVK93_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            35112..36938
                     /gene="ltrA"
                     /locus_tag="IVK93_001022"
     CDS             35112..36938
                     /gene="ltrA"
                     /locus_tag="IVK93_001022"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6386329.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            37072..>37873
                     /locus_tag="IVK93_001023"
     CDS             37072..>37873
                     /locus_tag="IVK93_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6386330.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT        12813 a         6317 c         7894 g        10849 t
ORIGIN      
        1 aggggcggta cataaaaatt tattatttag attctataaa catgtgcaac ttatctaaca
       61 acaaagtata tattctacag aagtaaacaa atagcttata ttttaggatg tacacaaatt
      121 gacaagtata ataaaacttt gataaaatac aaatgttttt tatttttatg tgtgattagt
      181 tagaacttac ttaaatccta ttataatatt tttgttaatg attttaataa aatgattgga
      241 gagaaaatga tggatcaaaa agcgtttatc gaatctattg attctacaat cgatagatta
      301 aaaaaagaaa ttcgatttta taatagagta attggtatcg gaaatattat aaaaatagtc
      361 ttatcagcta gtatcccaat attaattgat caggcttccg aacacaggtc tttactattg
      421 attgtttcaa ttgcttcagc aataattaca attatacaaa gtgggatgtc tgcatttaat
      481 tatcaagata aagtacagac ttcgacagag ttattaatga aaattgaaaa agaaaagttg
      541 ttatatataa cgaaaacatc cccatataat aaaacagatg aagaaaattt ccatttaatc
      601 gttacaaccc ttcaaactga attaaatgat attatctctg attttaatca agttaataac
      661 taaaaattta tttaattaga aagtagtgca gataatacta attgtacaat taagaaaaga
      721 gataagactt tacattccta aaaaaatgta aagtcttatc tcttttctta atgctataaa
      781 ttcataataa cattatggac aataaattta aaatttattc agactgtaga caaactccta
      841 aatttagagt gttgtttgca gtctgacgac tcaaaaatga gtcgtataaa tcaattatag
      901 taataagtca ttgatttctt taaattcttt gtcaagttct ttttcatcat atttttcata
      961 tttatcagct tcatgaagaa cttttttaat ttcatgaata gcctttcgtt caactaacca
     1021 tttttttagg ttatgttttt tttctggatt ttcagataac gtatcaagtt cgtctaactc
     1081 tttatccagt ttattgacaa cagaaacaat tttgtttaca actttttctt ctttgttttc
     1141 taattttgac atttatcttc acgtcctttt tatttgataa tttaagtata gaactttgat
     1201 atagtttata caaataaaaa cgcttctttg tgtattctgt gcttttcttt tggaatgagg
     1261 aaaactttgg agtaatatga acgtggacag aaaaatattt aggaggaata aactatgtgt
     1321 acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga ttatgaaata
     1381 tcttacaatg aagtagtcac tgttactcca agaaattata agttgaattt tcgaaaggta
     1441 aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc tgactaccct
     1501 ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa tttttctggg
     1561 tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt tgaatttatt
     1621 ccttggattt taggacaatg ctcaacagta ggagaagcta aaaaattgtt aaaaaatatc
     1681 aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca ttggctatta
     1741 gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca tatatatgat
     1801 aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt taatttaaac
     1861 aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca aataagtttg
     1921 aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc ctcagtatct
     1981 cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc agagtcagaa
     2041 agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg tttgtgtgat
     2101 gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt tgacaaagga
     2161 atctattatt atcgaacata tgaagacagt caaattactg caattgatat gaataaagaa
     2221 gacttagata gtcataagtt aattagttat ccaattatag aaaaacaaca aattaaatat
     2281 ataaattagt taatgtgttg tgattgatta tttaatatag atataataca aaaaggcaaa
     2341 gatattttca aaacattttt tcttgatgtg atatccttgt cttttttgta atgtttttaa
     2401 cttattgctt ttgcaaaatt tatttacctg ttttctggat caacgatagt gtatattatg
     2461 ctatccttgt ttggatttta ggacgtttaa agaggcactt ttttacataa cttaatccat
     2521 agacctttta agcatgctgt gagataataa taaagaagag tttaaagcgc accccaaacc
     2581 acttccccat aagtgtatta cgctttaaac tcttttatat ttgaattcac taagaagcat
     2641 accatatttt tgaaaaaaag tgagaaaaaa ggcttataat taggatttag agtaattaat
     2701 tagatgttta ttttatagga ctgatattat agattacaaa tagtatcgta tttcgcaatc
     2761 ttaaaattat cttcttacta tgaataagat aataaaattt gtatttgtcc tttttaggta
     2821 acttatttta atctatatac ctttctggat caacgaaagt atactttata tagtcataac
     2881 gccgatggta actatgcact gtaacgataa tgctgctaaa atatcttttt acgaaaaaca
     2941 tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt atttaatatt
     3001 taattaggag ggaatatttt gcttttttat agtaatttta tcttaatagt agcgatatta
     3061 ttacttctga atatatggat ttttgatagg agtagaaatg caagtatagg ttttagaact
     3121 aaacgaagtc tgtcttctaa aaaaaattgg gtatactcac aaactatttt ttatggagga
     3181 attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat agatgtttcc
     3241 acctcaaatt caatatctat tattggaatc attattgctg caattattac acaactgttt
     3301 ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata gttttggtga
     3361 tagtgatatt aaaaatcaga aaagatagaa aaagcaggag atagataaaa tgctgatatt
     3421 tcagcatttt ttaagagtat aaaagagaaa aaaagtaata aatgtataca tgatataaga
     3481 aaaacatata tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt
     3541 tatggatgcc gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt
     3601 ggaactaagt aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag
     3661 aagtaacgcc attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata
     3721 ttggcaatga ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga
     3781 gataaaaata tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag
     3841 gcgaaatgca cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt
     3901 atcatctttc atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattgaa
     3961 tcctgcaata atcaattact ttttcattat ttctagatta aatgaataaa ctctcatgcc
     4021 attatctcta aaaaaaaagt ttttccccct aattttttta gatagaaaat tgtatagtat
     4081 gatgagagaa aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc
     4141 tttatttatt tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg
     4201 ttgtacaagg tgcggagata ttaggcctga tccaaggaag cggatttgtg gtttctagga
     4261 attttccagc tttgactgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat
     4321 ctctgatcaa tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat
     4381 ctatgttttc tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat
     4441 tgaaggattc agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag
     4501 taattgcctc atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa
     4561 gtgaacgatc tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaagcat
     4621 atgagctgcg taaaatgcat gtttctcata tgcttttttt attattatgt aaagaatgcg
     4681 aaaaacgttt acatgatttt tacataactt aactatattt ttacagcaac catacgaggg
     4741 atttacagtc gactgataaa ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa
     4801 gtattgttta ctctaggtat gatgggaatt cttttagccg gatgcggctc aggatcagga
     4861 aatacagatg gctctgccac acaaaataat tcaagtgact caaatgaaca agtaaaaatc
     4921 gttgcggtag gttcgacagc attacagccg ttagtcgatg cagcacaaga aagctttgta
     4981 caagaaaatc caaattatca aatctctgta caaggaggag gaagcggaac tggtttgagc
     5041 caagtcgaag ctggagcagt tactatcgga aactctgatg tatttgcaga agaaagagac
     5101 ggagttgatg cttcaaaatt agtggatcat aaagtagcag tggtcgggat ggcgccaatc
     5161 gtcaataaag atacagatgt caaagatatc accaaacagg aattgattga tatctttaca
     5221 ggaaaaatca ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac
     5281 cgtgcaaacg gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc
     5341 cctgtgcagt cccaagaaca agattcatca ggaacagttc ggcagctggt aagtcaaaca
     5401 ccaggagcaa tcagctattt agctttctca tatctcgatg attccacaca agcactaagt
     5461 atcgacggtg tagaaccaaa agaagagaat gtagcagata acagctgggg aatctggtca
     5521 tatgaacata tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt cttagactac
     5581 atgatgacag aagaaatcca agaaggacct gtcaaagaat tgggctattt accaatcaca
     5641 atgatggaag tagaacgtga tcacgaagga aatatcaaat aactagcaga taaacatgcc
     5701 acggtagtcg ttactattaa ggaaaaatca actatgtttc ccacatatac atttttcctt
     5761 taattgtgaa aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc
     5821 tgattaaaca ccgtttattc gaaaaaaaga gctacgacat agaactgtcg tagctctttt
     5881 tttgctatat gacaggaatg aaaggtgttt gcaagcttaa tgtgaggaac actttccata
     5941 tctgttctat actggatagg agaagtttgg aaatatccag aaaacgaaat caatcgaggt
     6001 agccacatga agaataaatg gataaatcga agtaaaaaaa agaaagtaac acaaagatca
     6061 tcagataaag tcatccgtct ttttccatcg tataaagaag gattaacggc agatcaagtc
     6121 aaggaacgaa tagaaaaagg tgcagcaaat aattcagtcg atcccacatt caaaacaaat
     6181 cagcaaatcg tattggaaaa catatttacg tatttcaatt tgatctttct tatcttagcc
     6241 attttgcttt gtttagtaga atcctataag aacttgactt ttttaccagt tatcatagcc
     6301 aatacaggaa tcgccatttt tcaggaaatt cgatccaaaa agatcttgga cgaactaaat
     6361 gttttaaata cagcaaaagt caatgtcata cgggacggga tagaacaaac aatagatata
     6421 gaagaccttg taatagatga cattgttatt ttagagactg gacatcaaat tccagcagat
     6481 gcagaggtcg tggaaggaaa actgcaagtt aatgaggctt tattgacagg cgaagcagat
     6541 gaaataacca aagaaatagg cgatccatta atgtccggca gtttcattgt ttccggaaaa
     6601 gcttacgccc gattagacaa agttggagca gattcgtata tttcccagct gacagtcaaa
     6661 gccaagacaa tgggagaagg agaacagtcg gagatgattg cttctcttaa tcaattgatc
     6721 aaatggattg ggatcatcat tattcctatc ggtatcctgc ttttttctca aagttacttt
     6781 tttaatggaa acacgatcaa agaaagtgtc gttgcaatgg aagcagcatt gatcggtatg
     6841 atacctgaag gactgtactt gctgacgacg attgcattag cattaagtgc gactaaacta
     6901 gcaaagcaaa gagttctttt acacaatatg aaaagcattg agacattggc aagggtaaat
     6961 gtcctttgtg tagataaaac aggaacgatc acagaaaata aaatgtctgt tcaaaaagtg
     7021 atcgtctcta aaaaacaaga aataatcagt cagtcagatc aattggaagc atgcattgct
     7081 gactatgcaa aagcgatggc aggagacaat gcaacaatgg aagctgttaa agcatatttt
     7141 acaaaaacca ctgatcaatc ctatcaccat gttattcctt tttcttctgt ccaaaaattc
     7201 agcagtgtag cgttggcaga caaggtgtat gttttaggag cgcccgaaat ggttttgcgc
     7261 gatcaattgc cggaatatgc ggaagagttt gtttctttcg ctgaaaaagg ctatcgcgtg
     7321 ttggtctttg gtgtgtacga tgggattctt gaagaaccag tattgacaga agcagtcaca
     7381 ccattaggct atattctgat tgctaatcct atcagaaaag aagcaagagc cacatttgat
     7441 tacttcaaaa aacagcaagt agccatcaaa gtgatttctg gagataatcc gctcaccgta
     7501 tccaatgtag ctgtccaagc agggatttca catgctgatc agtacgttga tgcatctaaa
     7561 ttatcagaag aagaatatga agcagctgtc gaaaaatata cagtttttgg acgcgtcaag
     7621 ccagaacaaa agaaaatctt cgttcaattg ataaagcgta acaatacagt agctatgaca
     7681 ggtgatggcg tgaatgatat tctagccatg aaagaagcag actgcagtat agcgatggct
     7741 tccggaaatg aagccgccat gcaggcctca caagtggtct tacttgattc tgacttttca
     7801 cgcatgccag aagttgttgc agaaggacgg cgcgttgtga acaatattga gcgatcagcc
     7861 agcttgtttt tagtgaagaa tattttctcc tttttgctct cactgttttc agtgatcttt
     7921 gctctgacct atcctttaga accatcacaa atcacattga ttagtttgtt tactatcgga
     7981 cttccatctt tcttgcttgc tctagaagaa aacaaaaagc gaatcagagg gaaattcatc
     8041 atgaatgtga tggaaaaagc tgtgccaggt ggactaacag atatgatcgt agtcggtgcg
     8101 ttggtgatct gcggggtgac tttgaatcta aataaaacag atgtttctac agcatctacc
     8161 atgcttttga ttgcagtagg gttcctcgtt ctctataaaa tctgttcacc gctgaataaa
     8221 ttcagaagtc agatcattct tttctgtgct agcggtaact ttttttcggt catctttttg
     8281 cataagctgt tttcaatcac cggtatctca gctgtatcac ttttgctgtt gagcattcta
     8341 tttttctcgg cagattcgat ttttaggcat ttgaccacat tggtagaata tttgtttgaa
     8401 cgtaaaaatg aaaaagagca aaagccgttt agtcgcgtag ctaaaaaatt tctatcgata
     8461 tttaaacgag aaaattgacg aaaagtatct caaaaaagag taaaaaagaa caaccactcg
     8521 tgtatctttc gaagcaggat gagtcatcca actttaaaac gcacgagcag ttgctctttt
     8581 ttacattttc ggaatggtaa cggtgaaagt cgaacctgtt ccaagatgac tgtctacagt
     8641 aatcgtacca ttaaggagtt gcacgtagtc ttttacaatc gctaacccta atccggtccc
     8701 gcctgaatgt cggcttcgcg ccttgtctac gcggtaaaaa cgctcaaaga tccgttcctg
     8761 atcctcaatg tcgataccta taccaaagtc ttggacagaa aacgatagat catctgttgc
     8821 ctgatagcgg ataataatct cgctgtcgct ttttgaatat tggacagcat tttcaatcag
     8881 atttttgata attggataaa ggatatctgt tttagtagta aatggttcgt tctttccaat
     8941 caatcgaatc gtcaaccgtt tcttttcgat gattggctga taattttgaa cgatttgctg
     9001 gaaataaggt tctgtataaa ttgtctggat ctcgtaagaa ttctcgccat cttttgacag
     9061 ctggatgatt tcccgaatca gtttatctaa ccggatcgca tctttttgca tgatctcaag
     9121 aaaagaagtc agagtttgcg ggtcatcttt tgcaccatcc agtaaagtct cggtaaaacc
     9181 aatcagtgaa gtcacaggag tttttaattc gtgagagacg ttgccaacga aatccttttg
     9241 tagcttttct aaacgtcgca ctttcgttaa gtcatacgcc acccctagaa tctgtccggt
     9301 accttctgaa ttagtaaagt agcgcaaact caagtctaat gtctgccgac cttctgtaag
     9361 ggtgatctcc tgatggatca gcggatgctc aggagtcact tgatgaatca gctgaatgag
     9421 ttttggctct tgaatgactt ctgtgtaccg ctgttcaggt tgatagctta ccacgtttaa
     9481 atgtagctgc atctttggat taagcagtag taaacgagaa tcagcatcat caatcaggaa
     9541 tacgccaatc atcagttcgt tcagtaacgt atataattgc tcttctgtgg tagtacacgc
     9601 ccgatacatc ttactcattt gttcgctcaa cgcattaatc gtctgataca attcttccca
     9661 ttgatcgggt gtttgcatga ttacttccgc ctgattagga ttttgcacca ttttttttag
     9721 tacgggcagc acggttttca atggctggtt tttttgatag atcagataat agatcattcc
     9781 tgcaagaaca aggaagaaag acagaaagaa gaaaaagacc caacggcgaa aactctcagt
     9841 tctcggtaaa aaaccgcttg tcggttctgc gatacgtaaa atggcttcta actggccact
     9901 ttttttgact ggtaatgcga catataaaag atcctcattc aaagtagtac ttttgcgcaa
     9961 agcgctacct aggttcccac cattcaatac ggctttgatt tcgggacggg agtttcgctg
    10021 ttcattcaat gtttcattgt ttgtgtcaaa taagatcttt ccagtagcat ccattaaagt
    10081 aatccgttcg ttcgactgat ggacgaattc ttccaaagca gttttgtttt gcgctgcttg
    10141 gaggttttcg acatctaatt gccggatcag cagttgtgct ttttttgtca ggtagctttc
    10201 ttgctgttcc acgatctgct gttgaaaata atgggaaatc aattgccagc caccaaaaaa
    10261 caaactaact aaaagaataa gaaagaagag acgttcataa atttttttcc atttcatcgt
    10321 tttggttcct gaaacttata gccaaatcct ctaatagtca ccaagtactt tggatgtttt
    10381 ggatctcgct cgattttttc tcgcaaatga ctaacgtgta catctacgat tcggctttgt
    10441 cctgcgaagt caaaattcca aatgcggtct agcaatgtgt ctcggtcgat cacacggtct
    10501 tttcgtttca tgaaataaac aagtaattca aattctttcg gtgttagttc aatcagatgg
    10561 tcgtctacgg tcacttgata attggtcaaa tcagcagtga tttgtccaat cgatagatat
    10621 tcaggctcag cttcatcttt tgattgctta cgaggttcaa tccgtctgaa tattgctttc
    10681 atacgggcta atacttcgcg aggactaaat ggtttggtca aatagtcgtc agcacctatt
    10741 tctaggccaa tgatccgatc cacttggtca tctttagccg tcagcatcaa aatcggtgtt
    10801 tcattttttt cttgacgcag ccgctgagtg atctccatgc catccatcgt tggaagcatc
    10861 acatctaaaa tgatgaaatc aaatgattgt tctaatgcta attctaatcc ttcgcctcca
    10921 tctgtggcac tggttacctt gtagccttct ttttccaaat tgaacgtcaa gagcgttaca
    10981 atagaaggtt catcatcaac aactagtatt tttttcattc aaatctcctt taaataggtt
    11041 tcattctgtt tatattttag catagaatca gtacaaaggg ctattttaca aaagaaataa
    11101 atggtataat aggaaggcac tttgctgaat agaaaggaag agcagtcaat gattggaatc
    11161 agtgcatgtt taggcggtct ggcctgtcgc tatgatggac agaaaaaaga agtaccagaa
    11221 ttgatggaac tagttgaaac tggagaagct gtcatggtct gtccagaagt gatcggcgga
    11281 ttaccaattc ctagagatcc cgctgaaatc attggtggaa atggattcga tgtgtggaaa
    11341 gatcaagcaa aagtatggac gacaagcggc gaagatgtaa cggaagcgta caaaaatggc
    11401 gcgatcaaag cctatcaaaa attgcaggaa aaacacattg atctagtcat catgaaggaa
    11461 aaaagccctt catgcggaac ccattcgatt tatgacggaa gtttttccgg aataaaaaaa
    11521 aatggtgccg gagtggctac tgcttatttt attcagcaaa aaatgactgt gctctctgat
    11581 gaagaatggc tgaaaactag aggtgagcag aatggaaatc gaaaagacca atcgaatgaa
    11641 cgcgctcttt gaattttatt cgaccttatt gacagaaaaa caaatgaact acatggaatt
    11701 atattatgca gatgattttt cattaggtga gatcgcggaa gaatacgaag taagccgaca
    11761 agcagtatat gacaatatca agcggacaag caagatcttg gaggattatg aaaaaaaact
    11821 ccatcttttt tctaattata ttgttcgaga acaggtattg gaaaatatga ctgcatatat
    11881 tacagataag tacccggaag ataaaaaact acttgaatat gtacagcaaa ttcaagcaat
    11941 cgaagaataa ataaaaggaa tgaagaaaat atggcatttg aaagcttaac tgaacgtctc
    12001 cagcaggcga tgggaaaaat aagaaaaaaa ggaaaagtat cagaagctga cgtaaaagaa
    12061 atgatgcggg aaatccgcct tgccttacta gaagccgatg ttaacttgca agtagtcaaa
    12121 gattttacta agcgcgtacg cgaacgtgcg gtaggggcag aagtgctgga aagtttatca
    12181 cctgcacaac aaatcgtaaa aatcgttgat gaagaattga cgatcacact tggttctgaa
    12241 acagcagagt taaataaatc accaaaaata ccaactgtta tcatgatggc tggtcttcaa
    12301 ggggcaggta aaacgacctt tactggtaaa ttagccaatt atttaaagaa aaatgaaaat
    12361 gctcgtccgc tcctaatcgc aggggacgta tatcgaccag ctgcgatcga ccaattgaaa
    12421 gtattaggtc aacagctaga cgttcctgta tttgatatgg gaactgaagt cagcccggta
    12481 gagatcgtcc gtcaagggat ggagttagca aaagaaaaga aaaacgacta tgtattgatc
    12541 gatacggcag gacgtctgca tatcgatgaa acattgatgg atgagttgaa acaaatcaaa
    12601 gaattgacgc aaccaaatga gattttgctt gttgtcgatg ccatgactgg tcaagatgcc
    12661 gtcaacgtag ctgatagctt caaccagcaa ttaggtatta caggggtcgt catcactaaa
    12721 cttgatgggg atactcgagg cggggcagca ctatcgatcc gctcagtgac aggcgcacca
    12781 atcaaattca tcggttctgg tgaaaaattg actgacttag aagtattcca cccagatcgt
    12841 atggctagcc gtatccttgg tatgggggat atgctgacat tgattgaaaa agcgcagcaa
    12901 gactacgacg agaaaaaagc ggaagaactt gcacagaaaa tgcgggaaaa ttcattcgat
    12961 ttcaatgact ttattgaaca gcttgatcaa gtgatgggta tgggcccact ggaagatcta
    13021 atcaaaatga tcccaggaat gaatcaagtc cctggaatcg aaaacgtcaa ggtagatcca
    13081 aaagatgttg aacgcaaaaa agcgatggtt tattctatga cgccagcaga aagagcaaat
    13141 cctgatctgt tgaacccaag ccgtcgtcgc cgaattgctg ccggttctgg aaacagtgtt
    13201 gttgaagtga atcgaatgat caaacaattc aaggaatcaa gaaaaatgat gcagcaaatg
    13261 tccaaagggg atatgaacat cccaggtatg gaccaaatgt ttggttcagg cgtcaaagga
    13321 aaactaggca aaatggcgat gaatcgaatg atcaagaaaa ataagaagaa aaagaaaaaa
    13381 cggaaataag atgaaaaaaa cagcttcggt acagactgaa aatgtctact gccggagctt
    13441 ttctatcagc aatttttatt ctttactaat acaattactc taaacgaaga ggtggcacag
    13501 atgagatgtc cgtggagtga acaaacagaa agtatgcggc aatatcatga tactgtctgg
    13561 ggtgttcctg aatatgatga ccaaaagctg ttccgaaaat tgatgcttga tattaatcaa
    13621 gccggattga gctggcagac gattttaaat aaaagtgaag cattcgatga agcatatgat
    13681 cgattcgata tcaaaaaagt cgcaaattat ggagaaacaa aaatcagtga actaatggaa
    13741 aacaaaggaa ttattcgtaa ccgaagaaaa atcgaagcag ccgtccaaaa cgccaaagca
    13801 gtcttgaaaa ttcaagaaga aatcggcagt ttttcagagt atctttggtc gttttctaat
    13861 ggaaaaatcg ttgattcttc ttatcattca caagaagaag tcccaacaac aagtcctctt
    13921 tcagatgaat tagcaaaaga tctaaagaaa agaggattta aatttatcgg aagcacaaca
    13981 atctatgctt ttttagaagc tgtcgggatc atcaatgatc accttgacag ttgtttccgc
    14041 aaagaagagg tcgtacgtat cggcagaaag ggcgaataaa gcatgcgaaa aatcatattt
    14101 tatggtgcta taagtttaga tggctatttg gcaacgaaaa cggatgatct tcaatggctt
    14161 tttgacactc caacaggtaa gcaaactact tatgaagctt tttatgaaac aatcgatacg
    14221 accatcatgg gcagaaaaac ttatcaggaa gcaaaaaaat atttggagac cgaagcaatt
    14281 tatccagaga aaaagaattt cgttttttcc acccacgcac aattacaatt gccagatgct
    14341 gaggtcgtaa acgaagatcc tgttgctttt ctaaaaaatc tacaaatgac agaaggaaaa
    14401 gatatctggg ttgtgggtgg cggtcgattg atcaaaccgc tattggaaca tcaaatgatt
    14461 gatgaatggt atatccagat tacccctgta ttattagggg acgggattcc tttattccaa
    14521 aaaggaacat atgaagcgcg ttttgaaatg ctagacacga cacgattcgg tgaatttatc
    14581 gaattgcatt atgtacgaaa gtagaagtaa ataaaataaa aaaggcaact atatgcagaa
    14641 atgtcctgta catagttgcc ttttttatta ccgttatgct ttttactctg ttaatttgtt
    14701 agaaaaatct agatacgcat ctaaaatcgt ttggaagaaa cttaatgttc cttcaacgat
    14761 attaccattt tcatcaatta gattcgcaac gtttccgata tatgcttccg gttgctgcaa
    14821 ggttggcaca ttcaaaaaga caagtgattg gcgtagatgg tggtttgcgc caaagccgcc
    14881 gatcccacct ggtgatgcag taacgactaa acctggttta tgatcccaaa cactgtgtcc
    14941 ataaggtctt gagcctacat ctaaagcatt tttcaatact gctggcacag aacggttgta
    15001 ttcaggagta acgaaaacga caccgtctag tcccctcact tcctcacgaa aacgagtcca
    15061 ttcagcagga gcctgttctg gagtttcgag atcctcattg taaaatggca gatcatcgat
    15121 ttttacaaat acactttcgt acccttcagg aagcaatttc gcaaaagctt ctgctacttt
    15181 tttgttgtaa gaatcttttc ttaaactacc aataaaaaat ccaatttttt ttgtcatacg
    15241 atttttcctc cttcaaacta tctataataa tcttacaaaa gataagtaac tggagcaaat
    15301 aaaaacactt actgacaaaa gctacataga agatagatta cttattttta acaaaaaaga
    15361 agtctaaaat cttctatttt ctaaaaaaaa tcgaatttaa tcaagctgta aagaaaaaaa
    15421 gctttacagg gtaaaaaaaa actgttacac tacatcttgt gaataaaaca atggaggtgg
    15481 aaataaataa tggcagttaa aatccgttta aaacgtatgg gttctaaaaa gagtccgttt
    15541 taccgtatcg tcgtagctga ttcacgttct cctcgtgatg gacgtttcat cgaaactgta
    15601 ggtacttaca atcctttgaa agaccctgca gaagtagttt taaaagaaga cttagttctt
    15661 gactggttat caaaaggtgc acaaccttct gacactgtac gtaacatcct ttcaaaagaa
    15721 ggcgttatgc aaaaacacca cgaagctaaa ttctcaaaga aataaggtga ccggatatga
    15781 aagatttaag cgacttagtc ttaacaatcg ttcgtccgct agtcacttat cctgatcaag
    15841 tcaagttaga cgtcgtagag tcgaaagact tttacgagta caatctaaca gtggctcctg
    15901 aagatattgg ccgtatcatt ggaaaacaag ggcgtgttgc taaagcaatc cgcacaatcg
    15961 tttacggtgt tcggatcaat gcaccgaaaa aagtgcgttt gaatatcatc gatgataagg
    16021 aaaagtaaat aaacgtgctg tactcgaatt ttcgagtgca gcttttttgt tttttttatt
    16081 aattgaagaa atgattattt ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg
    16141 agtgaacagc caatgagaaa gaaaatgtta tttattccat ttgctctatt gttaagcgct
    16201 tgtagttcgg ttactcaaga atcagacaat acacatctat caagcagtat atctgaaact
    16261 gttaccagtc cagaaaaaag tagtaccaaa acggcttcca ccactgaaca aacaatggct
    16321 acctcaaata atgagaaaaa gactgcttta gaccagttga aagaacaaca gccaaatgtt
    16381 cccatgcctc tagatgtacc agtctcttca ggctatctca atatcgcagc gacacataca
    16441 aagcaaggtt attctatttt atattacaga acagatcgtc ccctcgggtt gaatgctgac
    16501 gaattgaatc aagaaacgcc tattgcaacc tatctatatc aatatgggtt tgcttctagt
    16561 caagaaacaa tccaagtttt gcagccattt gagatcgaca caaatggtca gcaagtggac
    16621 ttaggttctc gaatcacagg gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg
    16681 caagaaggaa actggcgtat tcgaatccgt gggaataata tagaaggcca agatccctta
    16741 ctattagcta aagaaatcgt agcttatttg gaagaaaaca gtctaccagc acctgaacaa
    16801 tttggtaaaa ttactgttga tatgggtgat acgacgaatc gagccgtaga agtaagctgg
    16861 caagaaccga aaaatgccta tacgatcact catcaagatc ctatgtctgc gttgaagatg
    16921 gcagtttcta tgaagaggtt gtgaaatcag cgcgctgatc tgagcccatc atttaactcc
    16981 aagtcacagg aagtaatcaa aaatagcacg ccgcattttt gcataatttt ggcttaatac
    17041 cttaaaagaa aactagaatt gcctcttcac acatgctaaa ataaaacaga caaaaaagaa
    17101 atggggatat aagttgactg aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat
    17161 caaaggcgaa gtccgtgtta tctcaacaac ggatttccca gaagaacgtt acaaaaaagg
    17221 aacggtgcta acactattcc aagacggaaa agcacctgtg gaattaacag tcaaaagtca
    17281 tcgcaaacat aaaaattttg acttattgag ttttgaagga catccttcta tcaatgatgt
    17341 ggaaaaatat cgggatggca tattaaaagt gtcaaaagac aactctgtag atctagctga
    17401 aaatgaattc tattaccatg agatcatcgg tctgcatgta ttagaagatg gaactgagtt
    17461 aggtaaagta aaagaaatct tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc
    17521 gaaaaaagct gatatattgc ttccatatat cgcttctgtc gtcaaagaag tcgatttaga
    17581 agctggagtg gtccgtgtag aaattccaga aggactgatt gacgatgaag attgatgtat
    17641 tgactttatt tccaagaatg tttgaaggtc ctatggggga atccatcatc ggtaaggctg
    17701 tagataaagg attattagat atcaatatct ctaacttccg cgattattca gataacaaac
    17761 atcagacagt agatgattat ccatacggcg gtggagcagg tatgctgctc aaagtccagc
    17821 ccatctatga taacatccaa gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa
    17881 ttcttcttga cccagctggt aaacgattcg atcagaaaat ggcagaagaa ttttcacaag
    17941 aagaacactt agtattcatt tgtggtcatt atgagggata tgatgaacgt atccgcacat
    18001 tagtgacaga tgaagtttcc ttaggagatt atgtccttac tgggggagaa ttaggtgcga
    18061 tggtcatgat cgatgcaaca gttcggcttt tgccagatgt gttagggaat cagacttcag
    18121 ctcaaacgga ttcttattca accggtttat tggaacatcc ccaatatact cgcccggcag
    18181 aatataaagg gatgaaagta ccagaagtat tgacaaatgg aaatcataaa ttgattgaag
    18241 aatggcaatt aaaagaatca ctaagaagga cctatcaacg tcggcctgat cttttggaaa
    18301 cacttgaatg gacacctcaa atgacgaaat ttttagaaga aatcaagaaa gaagaacaag
    18361 aaaaaacagc agatgaagcc aagtgacttt ttctaaaatc ttctttacag taggttgata
    18421 atatgttagt attttatggt gagtgcaaaa gcacttaact attacgatat tccgctgtga
    18481 gaaaagctca taagaatatt tggaataagg agaagaaaac catgaatcca ttaatcgaag
    18541 aattaacaaa agaacaatta cgttctgaca tcccagcgtt ccgccctggt gacactgtgc
    18601 gtgttcacgc aaaagttgtt gaaggtactc gtgaacgtat ccagttattt gaaggtgttg
    18661 ttatcaaacg ccgcggtgct ggaatcagcg aaacttatac agtacgtaaa gtttctaacg
    18721 gagtgggcgt tgaacgtaca ttcccattgc atacaccacg tgttgcaaaa atcgaagtag
    18781 ttcgctacgg taaagtacgt cgtgcaaaat tgtactacct acgtgcatta cacggaaaag
    18841 ctgctcgtat caaagaaatc agaagataaa cacggaaaac ttccttggaa atctgaaatt
    18901 tgtgaaaagt ccttgtatat caagggcttt ttcttttttc tctatcacag ttttaagcca
    18961 tttttggctg ttttggacca tttttcaatc atattccctt aaaaattgtc ctagattgtc
    19021 cgacattgac tatcagtgtc gtcaaaatag ggcaaaattt agtagtcagc ttgacgacaa
    19081 atataatacg aaaaatacca aaacatttac cgaatttagc aacttttgaa cccaagtttc
    19141 tcaaagggtc agctattagc aaaaaagaca aaacatgtac ttttctacta aaaagttgat
    19201 gtgataggct tctatctaaa taactattag gagtttttat cgtgaagcaa aatgatggac
    19261 gtatcatttg gaaaaatcaa agtgagttaa aactgatttt aacaattaat gaattcatcg
    19321 aaaaacacgg aattacatct agccgtcaat accagaaaaa gctatcagaa aatccaaatt
    19381 ctgcaccaag tatgtggttc attaacaaaa aatatggatc atgggagaat ttattgatta
    19441 gtataggacg agaaaatact gggtatggaa aatgggctag aatgtcagaa caagaattat
    19501 tggaaatagt agaagccttt attaagtgcg agaagataac ctctcaacga atgtacgaac
    19561 aaaaatcagt agggaagaat attccttcac ttagtaccat taaaaagatg ttaggagata
    19621 tacgtccact ttttaaagaa aaaaatgatg gatctcgatt tactgatttc gaactgctgt
    19681 tagaactaaa aaatgaaata atccgattaa aactacagga tgatttatca atgacaaagt
    19741 ttcgaaaact agttcaatcc ccaaaactac catcagttga tacaattatg aaaagaacga
    19801 acaaaaattg ggaggaattg atggcagaga ttggatttga ttatcgaaga atcaaaattt
    19861 ataagcagag aaacaaccta tccaagacaa agaaaactaa atagtatcac tctaaatcag
    19921 acgttcaaaa ttaaaatttg gacgtctttt ttattaccca aaaacgaaag gagatttatt
    19981 ttatgaaaaa gccaagattt aagaattggc gtttgctcgc aacactgagt ctattatgcc
    20041 aaacaattgg tggatcgctt ggcccaacga ttgcctttgc tgatgaaatt actcatccac
    20101 aaacggtaac tgtggaattg gatttggctc accagtatgc tgtcgaaggt acatttagcg
    20161 atggtcgtcc catgtcagaa gttaccgttc cgcactatgc agtttacaat ggtgtgaagc
    20221 aagatatttt ctgtattgag ccaggggtgc cgatttacaa tgagttcact cctggttatg
    20281 agaaaaatcc attgcctgat atgtcggaga aagcgaaatt agtttccgtt ttatggaagg
    20341 aagctggtac ggatgtggat acacatattg tggcccagaa gatgatttgg caagaagtta
    20401 atggctacac gcttcactca attaaacgtt ctgatggtag tgcagtaaat atcgcagcca
    20461 ttgagtcaaa aatcaatcaa gccattgcgg attatcaaaa gaaaccaagc ttccataact
    20521 ccacagtgaa aaccgtgtta ggtcaatcga ctactgtgac agatacgaac ggcttgaatt
    20581 tatcggagtt tgatgaagtt gtcgagaata tcgcaaatat tgattatcgt gtgaacggca
    20641 atcaactagt gattactcca aatgccaatt caaaagagag tggtgaatta accctaaaga
    20701 agtcagctaa tacaggaaca ccggtagctt ataaaatggc tggtcaacaa actttgatgg
    20761 cgggggcaat tgataagccc aatacctaca caatcaaaat tgatgtggaa actgagggtt
    20821 ctttgaagat taaaaaagtc gataaagaaa caggaaatgt tgtaccggga accgttttcc
    20881 atttagactt tggaaaaaca ttacctgcaa aagacgtgac caccgataaa gaaggcattg
    20941 cgacattgga tgagattcct catggcgcga aagtaaccat tactgaaaaa tcagtgccag
    21001 ctccttatac gattgacact acaccaatca ccgcaaccat taaatctggt gagactattt
    21061 ctgtgacttc aaaaaatact cgcgaaaaag gtcaaatcat tttgaataaa atgggggtgg
    21121 aaacaggaac tgatctttgg aacgacaaat actcattagc cggaaatacg tttgccattc
    21181 gcaaagacag tccaaccggt gaaattgtcc aagaaatgac cacggatgaa aaaggtcatg
    21241 cggaaacacc aaaagagatt gccaatgcgt tggaattggg aacctattat gtgaccgaaa
    21301 ccaaagctag taatggcttt gtgaatacct ttaaaccagt aaaagtcgaa ttgaactatg
    21361 tcaatcaaac cgtggctctt gttaccagta acgtaaaagg gcaaaaccaa gaaatcactg
    21421 gtgaaaccac tttgacaaaa gaagacaaag ataccggtaa tgaaactcaa ggtaaagctg
    21481 agtttaaagg agctgaatat actctcttta ctgcaaaaga tggtcaagcg gtcaaatgga
    21541 gtgaggcttt taaaccagag ttagtgaagg gaacgaaagc ttctgatgaa acggtgactt
    21601 tgactttaga tgaaaagaat caagttgctg ttaaacactt agcgattaat gagtattact
    21661 ggcaagaaac caaagcacct gaaggatata ccttggatga aacaaagtac cctgtttcca
    21721 tcaaaaaggt ggatgataac gaaaaaaatg ccgtgattac ccgagatgtt acggcaaaag
    21781 aacaagtcat tcgctttggc tttgatttct ttaaatttgc tggatcagct gctgggactg
    21841 ccgaaactgg cttcaatgat ctgaccttta aagtatcgcc attggaagga accagtgaga
    21901 ttacagatgc cgaagatgaa gcaattacag cttataacga gcaactaggt tttgatggct
    21961 atggcaagtt tgaaaatctt ccttatgggg attatttact tgaagaagta gaggctccag
    22021 aaggatttca aaaaattacg ccattagaaa tccgttctac ttttaaagaa aataaagcgg
    22081 attacactaa gagtgaatac atttttacga ttaccgaaaa aggacaaaaa caaccaatta
    22141 aaacggtgac cgttccttac gagaaactga cgaataaagc attttctgtt agcttgaatc
    22201 gtttgatgct ctatgattta cctgaagaag aagatagctt aacttctctt gggacttgga
    22261 aagacggagg caaagaattg acagaccttg attctaccga gctagttgat aaattgagct
    22321 ataacttgca tgaaatcaaa gaagactggt atgtggtagc gcaagccatt gatgtggaag
    22381 cgacaaaagc tgcccaagaa aaagatgaaa aagccaaacc agtggtaatt gccgaaacaa
    22441 ccgcaacgtt ggcgaataaa gagaaaacag gaacttggaa aattttgcat aaattaaccg
    22501 cagaacaagt tttggataaa accatcgtct tattcaacta tgtgtatgaa aacaagaaac
    22561 cctatgaagc aggtgatgat ccagtagcga aagatgctag cttgaacaat caagcccaaa
    22621 ctgtcaattg tacggtggaa cgccatgttt ccatccaaac aaaagcccac ctagaagatg
    22681 gttcgcaaac ctttactcat ggggatgtgg tggatatgtt cgatgatgtg tcgattaccc
    22741 atgatgtact ggatggctca aaagaagctt ttgaaacaat tctgtatgca ttacttccgg
    22801 ataatacgac caaagaaatt tggaaatctg gcaaaattga ttatgaagtg aatgacaaag
    22861 aattcactaa aaccgtactt gcggaaaaag tggacacaag taaatatcca gaaggtacaa
    22921 gttttacctt caaagaaatt aactatggca aagacggaaa catcaatggg aaacacaatg
    22981 aggatctaaa agaaaaaact caaactttaa cacctaaaaa agttccgaca acaccaagtg
    23041 caccagaaca accggaaata ccaactgttc caagtgactc tcaagaatct agtcctacag
    23101 taaagacatt cccacaaaca ggggagaaaa attctaatgt tctgctgttt attgggttta
    23161 ccttgatctt tgcgactgct gggtattatt tctggaatcg ccgaaactaa ggtgatgtga
    23221 tgaaacaact aaataggaaa aaccaacgtg cgcgaccgcc tcctaaaaaa agtggtcgcg
    23281 ctatttttgt atccaaaact aaaaatgaaa agaggaaaag aagatggaat tgaaatttgt
    23341 tgtgccagat atggccgaaa cctttggaaa gataaggtat gccggcgaag gcgaagtttt
    23401 aactgaagga tatgggcgga atactacagt aattggtcgt agttatcatt tgtattccag
    23461 taagcaacga gccgatgata ttgaagtggt ggtagctgcg gaagccggcg aaaaggattt
    23521 tgatcaagat caaccactaa aagccgtgaa tccccatttg gttgccaaag gctatgagat
    23581 tgagaatcgt gggtttaccg actacgtgtt gtatgtcgat gatttagtga aagcatagga
    23641 ggaaaagaag atgagattaa cagaaggtat tgttgtagat tcaggactaa cgtttgggaa
    23701 attacgattt tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacggttag
    23761 taacgaggta aaagaacgga cttataattt gaaatcctct gcgcaaggtc gaatgatcca
    23821 agttagtatc ccagcaaatg tacccttacg cgagtttgcc tatgacgcag aggtagagtt
    23881 ggtcaatcca atcatggata cggtcgccaa ttatgttttc cgagaaggaa ctactgtcaa
    23941 ttggttcatt aaggccgatg atttagtctt gaaacgacaa ccagatcagg gaaattctac
    24001 aaaccaaaat gaaggaaaga aataggtgaa gcgtatggaa attaatgttg aacatatttt
    24061 agattgtctc gatcaatatg gaaaaggtga gctaacggag gaacaactca ctaaagcatt
    24121 aacctatgat gaaaaaatgt ttctgattat gcatcaaggg ttactggaag taggtaacga
    24181 tgccgaagaa gattttgatg tactgaattg gttaggggaa gaagagtcct tctttatggt
    24241 tatcgaagta aatgaaagtc tatgtcgaga agccgaaacc gtattggaag aaattggtgt
    24301 ggaaatgccg gatgccattg aagttttttt aaagcaacta gtggaaacga aacaactccc
    24361 tatagcagta aatgactagc ataactgctt gaaattggat caatgaaatc ctatgaagct
    24421 tttttcgatt ggaggtggaa accataaaaa tgtacaaagg gcatcgtata cgggcaggag
    24481 atcagcactt ggtttatcac tttgttcttg gctggctact cgctctgttc atcggttgga
    24541 tgagcgtctt ttattttcaa gagcttagac aatttgatat ttctaagcta tcactttcta
    24601 ctatagaaat cgttcgttct ataaaagatt taatctattt gctaggaagt ctcgttcttt
    24661 caggaagcac gatgttgctt tatatacact tttttcagga tcattggcgc agtttgtggc
    24721 atcgacaaaa gctagcccgg atgattctag aaaatcattg gtacgaagta aaacaaactc
    24781 agagtgaagg atttttcaaa gacctcaata gtagtcgaac caaagagaca atcagttact
    24841 tccccaaaat ctattaccgt atgaaggatg gtttactttc tattcgcgtt caaatttcac
    24901 tgggaaaata tcaagatcag cttttaaagc tggaaaagaa gttagaaagt ggtctatatt
    24961 gtgaactagt agaaaaggaa ctcaaagact cctatgtgga gtacacttta ctgtatgata
    25021 taattgccaa tcgaattggg atagacgaag tagtggcaga aagtggggct ttgcgattga
    25081 tgaaaaatca agtatgggct tatgattctt tacctcatat gttaatcgct ggcggcacag
    25141 gtggtgggaa gacttacttt ttactcacta tcatcgaggc tttattaaaa tctgatgccg
    25201 agctttttgt tcttgatccc aaaaatgcgg acttagccga tttaggtacg gtaatgcctc
    25261 atgtctattc ccaaaaggag gaaatttctg cttgtgtgga agatttctat gagcgcatga
    25321 tgactcgtag caaggcaatg aaagaaatgc ccaactacaa aacaggagag aattatgcgt
    25381 ttcttggact tccaccaaac tttttaatct ttgatgaata cgtggtttat atggaaatgt
    25441 taacgacgaa ggaaagtgcg gtgattttga ataagctcaa acaaatcgtg atgttaggtc
    25501 gtcaatctgg tttctttctg attctagctt gtcaaagacc ggatgcgaaa tatttaggag
    25561 acgggattcg cgatcaattt aatttctggg tggctttggg tcgtatgagt gaactgggat
    25621 attccatgat gtttggcgag gttgataaaa aattctttat gaagcgtatc aaaggtcgag
    25681 gctatgtaga tacgggaggg agtgtgatta gtgagtttta tacgccactt gtaccaaaag
    25741 gatatgattt cttaagggaa attagcgaca ttggtggatt aaacgttaat acggaaggat
    25801 aaaataatag tatgtagcct aggagtgtca agaatcctgg gcttttttta cgaggtgatt
    25861 tatgacattt ttagatttat tcgctgggat tggtggcttt tgcttaggta tggaacaagc
    25921 gggtcaccag tgtatcggct tttgtgaaat tgatgatttt gctcgccaga gttataaggc
    25981 aatccatgat acaagtaagg aggtggaaat gcatgacatc acaagtgtat cagacgagtt
    26041 tattcaatct ctcggaccag tggatatcct ttgcggtgga tttccgtgcc aagctttttc
    26101 aattgcggga aagcggcaag gattttccga tactcgaggt actttattct ttgaaatcgc
    26161 tcggttcgcc gctattctcc aacctaagtt tttattcctt gagaacgtcc ggggattact
    26221 caatcacgaa ggaggggcta cgttcgagac gatcctccga acgctggatg gattggggta
    26281 tgatgtggaa tggcaagtgc ttaactcaaa ggcctacgtt ccccaaaacc gtgagcggat
    26341 cttccttatc ggacattctc gagacgcatg taccgaacaa gtatttccta tcattggatc
    26401 gtctccaaca tctgatcaaa acatcagaaa cttattaaat atcaaccctt ccaatcgagg
    26461 aatgggggga caagtctatg ggtcagatga cgtagcgcct accttgaccg gtgatgaagg
    26521 aatcaaaatc gcattgccgg tagatgatgg aatttctgta gcgggtatgt tactgggaaa
    26581 ttttgaacaa ggcaatcggg tttatgagat aactggaaca gcgcctactt tgtcaacgaa
    26641 acaaggcgga acgaagatca tgcttcggaa aaacgaaaca gaatatcaag aggtgaagcc
    26701 agggaacagt gtgaacctgg cttttcctaa ttccacaagt cgtcgaggcc gtctgggaaa
    26761 acaaagtgtg cataccttat taacaggaga ccaacaagca gtcgtcacgg atcagtatca
    26821 aattcgaaag ttaacaccta gggagtgctg gcggcttcag ggctttccgg actgggcttt
    26881 tgatcgagct tctcaagtca attcagatag tcagttgtat aagcaggctg gaaattctgt
    26941 aacagttccg gtcatttttg atattgctag aagattaaag gaggtaaaaa cagatgattt
    27001 atgatgagtt gattggggaa tctactgggt gattggcaag attcagtcag atccggaatt
    27061 ggaagaagaa ctgtaccggc tcaattttga tattcgcaaa aacggggtga aagtccctga
    27121 tgatccgtat gtaattgatg aggaaacgga tgctcgaatg gagctgaatc aagtcatcag
    27181 tgaattggaa cgaattgcgg actttgctaa agaacctgat atccgccaat acttgtttga
    27241 gataaaagca gagttggaaa ttgagggtat taccgcggag taacggccaa ataaaccact
    27301 gataggcact gaccgcgggg gatttgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt
    27361 tatggacggc gttagccggc catgatgtct caccccccgt atctaacagg ggggtacaaa
    27421 aacacaaaaa ataagcaagc aacgataagg aatggctgtc ctatcaagga taataagcaa
    27481 tttgtgtaga tgttaactga tttgaaaaag ttaacacctt aaatttggag gtgagaactt
    27541 ggcacaaagg aatttagatt atcggctatt aaaagaccgg cgtaatgaat atggcgtttc
    27601 acaaaataaa ttagctacga cttgtggact tagtcgcccg tacttaaatc agatagaaaa
    27661 tggtggcgta accgcatcga caaaaaccat gaggaaaatt tttgaccaac tggaaagttt
    27721 caatcccgag ttacctttaa cgttactgtt tgactatgta aggattcgct ttcccacaac
    27781 ggatgcgagg aaaatcattc aagagattct ccatttgaaa tttgattata tgctccacga
    27841 agattacgcc ttttactcct atcaagaaca atatgttatg ggagatattg tggtgatgtt
    27901 gtcacatgaa gaagataaag gcgtcctttt agaattaaag ggtcgtggtt gtcggcagtt
    27961 tgaaactttt ttactcgccc aaaagcgtag ctggtatgac tttttcgaag attgtctaaa
    28021 agccggtggc gtgatgaaac gcttagacct ggcaatcaat gatcgagttg gcctgttaga
    28081 tattcctgat ttaacgaaaa aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt
    28141 caaaagctat cgttccgggg aactgttgaa acctgatgat aaagatggta tgggcaatac
    28201 tttgtatatt ggcagtctta agagtgaggt ctatttctgt ttgtacgaaa aagactatga
    28261 gcaatatatc aagttaggga ttcctttaga ccagaccgaa acgaaaaatc gttttgaaat
    28321 ccgactgaaa aatgatcgag cttatcatgc aattcaagat ttgttaaaag gtcgcagtat
    28381 tgaaagtacc acattttcca ttattaaccg gtacttgcga tttgcggata aggtggaagg
    28441 caaaaggcga actaactggc ccttgaatga acaatggggg cgcttcattg gacggaatca
    28501 gaaggaaatt caattaactt ctgaaccgaa gccttatacc attgaacgaa cgttgaactg
    28561 gcttggacga caagtcgccc ctacgtggaa gatggccaag gagttggatc ggttgaatca
    28621 gacaacgtac attcaggata tggtaaataa tactcgacta tcagaccggc acaaaaagat
    28681 tttggagcaa caaagtatgg caattgaaaa tttgattata tgaaaagagg aatcgtgatg
    28741 gaaattgtga aagatatttt attggtgttt ggtggcagta tgcttggtgt tactgtgatg
    28801 tgcttgatgc atgcaagtgc agcggcggac cgagcaatgg aaaagaaagg aaagtgatga
    28861 aatgaatttc ggtcaaaatt tgtatcaatg gtttttaacc aatgctcaat ctttagtctt
    28921 actagcgatt gtggtcattg gtttgttttt aggatttaaa cgcgagtttt ccaaactgat
    28981 tggcttcttg gtgattgctt tgattgcggt aggtctggtc tttaatgctt ctggtgtgaa
    29041 ggatgtctta ctcaatttgt ttaaccgaat tgtcggtgcc tgagggggaa tcacaatgga
    29101 acagatacgt gtttatattg cgaaccttgg aaaatacaat gaaggtgagt tggtgggggc
    29161 atggtttacc ccacccgtcg attttgatga agtgaaagaa cggattggat taaatgacga
    29221 atatgaagag tacgcaattc atgattatga actgcctttt gagattgatg agtatacacc
    29281 aattgaggaa attaatcggt tgtgtggttt agcggaagag cttaaaggaa cgccaattgg
    29341 tgaggtcgct tcagagattc aacatgcctt cttcaattct ttcgaggaaa tggtagagca
    29401 tgtggatgat atcatctatt atcccgattg taatgacatg agtgatgtag cgtattactt
    29461 gattgtagaa gctggtgatt taggcgaagt accagaacac ttgaagacgt atattgattt
    29521 tgatgcgtgt ggtcgagact tggagattgg cgggaattat ttggttacga gtcgaggggt
    29581 ttttgaacta atgtaaatca tttgttagaa ttgaccagaa atttctatcg tacttttact
    29641 tttctatttg atatactgaa atagaaaata tttgttgata ggggtgttac taatggttgg
    29701 caataagtgg tacaaatgtg atcttcatat tcattcaact gcgagtcagt gctttcagga
    29761 taaaaaggtt actgctaagg aatgggtaga agaatgttta aacaaaggac tcgattgtgt
    29821 tgcattaact gatcacaata caggggaaaa tattgatgaa tataagaaag tggctatcga
    29881 aaatggctta gatttttttc ctggagtgga attaacgtgt ggagaaaatg gaactcattt
    29941 actagtccta tttgataaaa gtgcatgtac aaatgatatc tctgatttct taaccaagat
    30001 gggtataaaa agagaggatt gggccagcgc tgcagcttgc tcaatgaaat cagttttaga
    30061 agttattgat tttgctgagc acgagggctt gctcgttatt cctgcacata tagatgagtt
    30121 taatggaatt tcttcagcaa aatttggaaa tctaggaaag atatttgaat cagaaaatat
    30181 atatgcagta cagacggttc aaaaagaatt ttttgaaaat cgctcacaaa tgattccctc
    30241 atctaagcgt gacactatct atgatagtgt gaatgccaga tatgatggta gagttggaaa
    30301 agatactttg gaatcttggt ataaatcagt tgttgaggca gaaaaaaata atatgacatt
    30361 tcttagcttt tctgataacc cacactcgaa aggaaattca aaacatggac tttggggaat
    30421 aggcacaaga tattcctata taaaaatgaa ggacgaacct gacttgggat cacttcgcga
    30481 tgctttaatg ctaggcgaaa ctcgagttca ttctgatttt tctaatttct ccattaatga
    30541 aaatgaagtt ttattggaaa aattaagctt ttctgggacc actcttagca caaaagaagt
    30601 tgtagtcgaa ttcagcgata atttaacttc aattattgga ggaagaggaa caggcaaatc
    30661 ttgtattaca agattcttgg tctatgtcct tggaaaggaa gcagaacttg atcaattttc
    30721 tgaaatacaa agtgattatc agaactttgc acaaattgaa cataatggct ctggaatatt
    30781 tttgaaggat acaattgtga aactgaatat tttctataag gggacaaaat atcaaataat
    30841 aagaacacag gatagacatt ctatttatga atttacacaa gagaatggtc tggttgaaaa
    30901 agaaacagaa cggttgagaa tgatttccga taaggtgagt atatactctc aaaaacaaat
    30961 ttatgaaatt tcaaaaaatc aaactagtat actagaatta ctagatggat ataactctga
    31021 tttgatttca gagtataaaa atgagattga aacatgtgta aacgaaataa gaaaattaaa
    31081 ttgggatatt gtttctgtga aaaaagaaat acaagataag gcgaaggtag agcttgaaat
    31141 tgaagattta aaaaaacaag tagaaaagct atctcataag agttataaag atgtttatga
    31201 tgagtattca aaagagacag atatttatag ggaactgaaa cgtgatgtgg aggcacttaa
    31261 ggaaattccc aaaaatctaa ctgatagtgt tgacaaaatt gactttggca atggtataaa
    31321 agttacagac gaaattgatg aacaccgcgg agaattgatt aagaatttag tgaccaatcg
    31381 ggctaagatt caaacaataa ttaatgaaat gtcagatgaa attgatagtt atagagcaga
    31441 gctgaataaa tcagattgga aagttaacta taatgaggtt tcaaagaggt atcaaagagg
    31501 tatcaaaaac ttagtgaaga gttatctcaa gatgaattaa aaatgttaca aaaccttgat
    31561 tctttatatc aatctttatc aaaaactgaa gagcgattgg aagaaattat tcaacaagaa
    31621 ataaagctta aagattacca agttgaggtt gactcagcaa tgtcaagaat gaataaatca
    31681 tatcaaaatt tctattctga gagaagtagt tcctcgcgcg aaattttttc tgatattaca
    31741 ggaatagagg ctactattca accaaatagg gatttcaata attatgttaa agagctcaga
    31801 gcttgtatta ataaggagga cacttttgac agtgagtttg gatctcttat tgataagctg
    31861 acaaaaaatt ccattactaa tcaagatata tataatgaca tacttgatat aagagttagt
    31921 ggggaaagta gtattttttc agataaaaaa ttaatcaatt ctataagaaa attgaatgat
    31981 gcacaagttc ttgaattaca aacccttaag ccaaatgatt tagtagttat ccgacttgaa
    32041 ctaaatggaa aaaaagttag tttatcgaat gctagtgctg gtcaaaagac atctgcaatt
    32101 ctttctatga ttttggcgta tggaaattca ccactcatat tggatcagcc tgaagatgat
    32161 ttggatagtc aattaattaa taacttaatc gtaagaagta tcattacaaa aaaggaaaac
    32221 agacaaattt taattgtaac tcataatgca aatatcccag ttaatggtga ttcagaatgg
    32281 ttgattagca tgggagattc aaaagaaata tcggctgatc agtgcgggtc ggtagatgaa
    32341 cccaatatca agccaagaat atgtactgtg atggaaggtg gcgaggatgc ttttacaaat
    32401 agagcgaaac gctatggttt taaacaaatt gtagaataac actagagggt gagacatatt
    32461 attatgtccg ccctttttag gaggaaaaat gaaaaaaata aaaagctata ccagtatctg
    32521 ggcagttgaa aaagtcattt acgcaatcaa tgattttcag ttgccttttc ctgtgacttt
    32581 taaccaaatg gcttggttcg ttttatcttt actgtttgtc atcgtatttg cccatgtgcc
    32641 tccactatct atgatagagg aggtcttttt aaaatactta ggcataccgg tggctgtcac
    32701 ttggtttatg tcgcaaaaga cgtttgatgg caagaaacca ctgggatttt tacgatcctt
    32761 tatcagctat cacttgcgat ttaaggtgac ttttgcagga aagaaagtca aagaacagaa
    32821 gaaacgatgg gatgaaccca gtactcttgt aaggagtgtg aactatgtat ccgattaagt
    32881 atattgaaaa taatctcgtc tttaaccaag aaggcgagtg ttttgcctac tatgaattag
    32941 tgccttacaa ttactccttt ttgtcacccg aacagaaatt tcaagtgcat gataactttc
    33001 gccaactaat tgcgcaaaat cgtgaaggca agatccatgc gttacaaatt gccacagaaa
    33061 gtagtattcg cgccacacaa gaacgatcaa aaaaagaaat taccggtaga ctcaaagaag
    33121 tagcgaaaca acgaattgat atacagacag atgcattagt atccatgatt ggcgatagtc
    33181 agattgatta tcgctttttt attggcttta aactaattgc gacagatgaa gaagtcaatc
    33241 taaagagtct caagaaatct ttcttttctg ggcttcaaga atttgtctat ggagtgaatc
    33301 atcatttgat gggcgatttt gtttccttat ccaatgaaga aattcgctgc tattctaagc
    33361 ttgaaaaatt aatggaaagc aaattggctc gaagatttaa agtcaggcga gtcacaccga
    33421 gtgacctgac gtatttgatt gaacatattt atggcgaaaa agggataccg tttgaagaat
    33481 acgaatttca gctaccaaag aaaaagctaa aatcagaaac actggtgaaa cgttatgact
    33541 tattacgtcc aaatcgttgt ttaattgaag aaaaaccaag gtgcttacgg atggaacatg
    33601 agaatcatga gtcgtacgta gcgtatttaa caattaatac aattgtgggg gagatggaat
    33661 ttccttcctc agaacttttc tattaccagc aacaacaatt tacattccca atcgacacaa
    33721 gtatgaatgt agagattgtg acgaataaaa aggctttagc taccgttcgt aataagaaaa
    33781 aagaactgaa agatttagac aatcatgctt accaatctga caatgaaacg aattccaatg
    33841 tcttagatgc tttggattcc gtggatgaat tggaaaccac cttggatcag tcaaaagaat
    33901 ctatgtacaa actgagttat gtggttcgag tgagtgccga atcagtggat gaactaaagc
    33961 gacgctgtga tgaagtgctc gatttttatg atgataccaa tgtaaaacta gtccggccct
    34021 ttggcgatat gatggggcta catgaagaat tcttaccctt aagtaaacga tatatgaacg
    34081 actatattca gtatgttacg tctgattttt tggcggggct tggttttggg gctactcaga
    34141 tgctaggaga attagaaggg atttactttg gctacaatgt ggatacaggt cgtaatgttt
    34201 acctgaaacc ggcattggct tcacaaggag tcaagggttc agtaaccaat gcgctggccg
    34261 ccgctttcct gggttctctt ggtggaggta aatcctttag taacaatctc ttagtctact
    34321 atgctgtttt gttcggtggt caagctgtga ttgtcgatcc aaaagggggt gcgaaacgtt
    34381 cctacttgaa aagagtaggc acagcacttc attaaatcaa tatttaatgt gtgttggaga
    34441 actaaacaca cgaatagtcg aatgacgggg taacgccctg aaaggctact ttaatactct
    34501 gactggcaga aacttgagta aacaaggctc tgtcagaagc tcggtgaagt cggcagacag
    34561 acaccgtaac agataatgct gacgaaagat tcccagaggt gggcgttgtg tcctatatgc
    34621 cggaagtcta taaaatatct atggttagaa tgtagatgag aggtctactg acgaatcctt
    34681 gaatgtacag gtctatatcg gggctggata gaaatgtcca gtgacacaaa cagtgtcgct
    34741 actgtgggtg agtaagactc cgcatttatg aaaatccata taacgttaaa ggcgttatca
    34801 aggtagcagg cttatagagg aaacctaagg atatatgtac agatagtgtg tttggaacgt
    34861 tgaaagctga caacatggag attttactgt ctgtgaagtg ttcacatcag aaagttattg
    34921 tgagattagc aatgatataa ggtgtgttaa tgtcagtgag agtggtggca cagtacctat
    34981 gaaaccgtga taacaagcgg tggagggata gccactagtc atatgaagaa tagaattact
    35041 aagaaaaatg gtatgggttc gagtaagact aagagatgta cttgctcctg caaacaagga
    35101 gggtacagac catgctaaag aaaaacaagc tccgctataa cgagtattat gatatgcaac
    35161 atatttatga cgagttatac gcacagagca agaacggtaa taacttttat aagttacttg
    35221 aaattattgg ttcggaacaa aatatttgtt tagcttaccg taatctgaaa tcaaacagtg
    35281 gcagtaaaac ggctggaacg gatggaatga cgatagacga tataaaacaa ctcagcaatg
    35341 ctgagattgt tgctaccgtt cgggaaagcc ttagcaacta ccgccccaaa tcagtcagac
    35401 gtgttttcat tccaaaagct gggagcgata aaatgcgtcc attggggatt ccgtgtatct
    35461 gggacaggct ggtacaacaa tgtatcctgc aagtcctaga gccgatatgc gaaccaaagt
    35521 tccataatca ttcctatgga ttccgtgcga atcgaagtgc ccaccatgca gtcagcagag
    35581 taacgacact gataaattta tcaaaatatc attactgtgt tgacgtagac attaaaggat
    35641 ttttcgataa tgtcaatcat ggaaaactat taaaacaaat ctggacactt ggtattcggg
    35701 ataaaagatt gatttgcatt atcagcaaaa tgctgaaagc cgaaattgac ggcgagggtg
    35761 tcccagaaaa aggaacgcca caaggcggtc tgttaagtcc actgctctcc ctcatagtcc
    35821 ttaatgaatt agactggtgg gtaagcagtc agtgggaaac attccagccg aagaaccgca
    35881 gtaaaaatgg gtggcttcaa tacgcaaaga aatataccaa actgaaaagc ggttttattg
    35941 tacgttacgc tgatgatttt aaaatcatgt gttcaaccta tggagaagca caaagatttt
    36001 accacagtac cgtagatttt cttaataaaa ggctgaaatt agaaatcagt ccagagaaat
    36061 caaaggtagt aaatctgaag aaaaattcat cagattttct ggggttcaaa atcaaagtca
    36121 ttccaaaagg aagaacgaaa cacggttatg ttgccaaaac agatatgaac caaaaagcat
    36181 taaagaaagc gaaaacaaat ctaaagttaa aggtaaaaga tatagccaga catacgactg
    36241 gcttcaatat cagcagatat aatctgacag ttatcggtat gcagaattac tactgcatag
    36301 caactaacgt atataacaac ctgacagaag taagttacgc tctcttaccg accatacgaa
    36361 tccgtttaag gaacattgca aaatcagttc catttgaaag tacaagttca gaatttcaga
    36421 gcaggacaaa aggaatcaga ccaaaaacaa aaatagtgat gattgcggat aatccgctat
    36481 tgccgattca aggagtacaa cacaaaaatc ctatgaattt ctcgcaagat atttgtaatt
    36541 ttacaaagca agggagaaac aaggtgcatg aggacgttgt agttgtaaca aaagaagaaa
    36601 tccgagcgtt gttggaaaat gaaaaccctg ccgatagcgt agagtttaac gacaatcgta
    36661 tttcggcata tatcgcacaa caaggcaact gctatgtaat gaatcgtcga ggaactccgt
    36721 ccactctgat atgtattcac aaatcagaca aaggagataa ccttgacaga tattctaatc
    36781 tggcatttgt ggaaattccc attgcaaagg caatcctaac agaatcagca gacgaagcaa
    36841 aatcactgtt aaaaggttat gttcttaata gtcagcagaa gaaaaaactg aacagaatac
    36901 ggacgaatta cggataccag acaataactg ggaaataact tataaatcta tttggatatg
    36961 atggaacgcc gtatgaagcg aaagtttcat gtacggtgtg aagcggggga aaacttggag
    37021 ataacatcaa aaagttacct atcgctatag cgaggagggt ggaaagaaac cttgccagaa
    37081 attgcggatg aaatcagtat cctcaacttg acgagtaagt cagaaaatca aggtctgctt
    37141 gatccttatg tgattatgaa gaaaaagaaa gactcggaaa gtttagcaat tgatattctg
    37201 acttttttaa caggaatttc aagtcgagac ggggagaagt ttcctgtgtt acgtaaagcc
    37261 attcgaacgg tgactcaaag tgatcaacgg gggttattac aagttattaa cgaattgcga
    37321 aaagacgggt caccaatcgc ggaaaatatc gccgatcata tcgagtcctt taccgattat
    37381 gattttgctc atttgctttt ctcagacggc tcgattaccc aatcgattag tttagataaa
    37441 cagctaaata tcattcaagt ggcggatttg gttttaccgg atgcagaaac gacttttgaa
    37501 gaatatacca ccatggaatt gcttagtgtt tcgatgctaa tcgtcatttc cacttttgcc
    37561 ttggacttta tccatagtaa ccgagaaatc ttcaaaattg tcgatttgga tgaggcttgg
    37621 agttttcttc aagtggctca aggaaaaacg ttatcgaata agcttgttcg tgctggtcgg
    37681 gccatgaatg ctggggtcta tttcgtcact caaaatagcg acgatttatt agatgaacgt
    37741 ttaaaaaata atatcgggtt gaagtttgct tttcgcagta ccgatattca tgaaatcaag
    37801 aaaaccttgg aattctttgg tttggatcag gaggatcaaa gcaatcaaaa acgcctgcga
    37861 gatttggaaa acg
//
LOCUS       DACCCR010000020        37540 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCCR010000020 DACCCR010000000
VERSION     DACCCR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.020"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..807)
                     /locus_tag="IVK93_001024"
     CDS             complement(<1..807)
                     /locus_tag="IVK93_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6386331.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPE"
     gene            955..2508
                     /locus_tag="IVK93_001025"
     CDS             955..2508
                     /locus_tag="IVK93_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6386332.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            complement(2616..3698)
                     /gene="uxuA"
                     /locus_tag="IVK93_001026"
     CDS             complement(2616..3698)
                     /gene="uxuA"
                     /locus_tag="IVK93_001026"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6386333.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3689..5335)
                     /locus_tag="IVK93_001027"
     CDS             complement(3689..5335)
                     /locus_tag="IVK93_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP6386334.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5519..6181
                     /locus_tag="IVK93_001028"
     CDS             5519..6181
                     /locus_tag="IVK93_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6386335.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            6325..6888
                     /gene="ahpC"
                     /locus_tag="IVK93_001029"
     CDS             6325..6888
                     /gene="ahpC"
                     /locus_tag="IVK93_001029"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP6386336.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            6898..8427
                     /gene="ahpF"
                     /locus_tag="IVK93_001030"
     CDS             6898..8427
                     /gene="ahpF"
                     /locus_tag="IVK93_001030"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP6386337.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            8584..8934
                     /locus_tag="IVK93_001031"
     CDS             8584..8934
                     /locus_tag="IVK93_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP6386338.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            9033..9563
                     /locus_tag="IVK93_001032"
     CDS             9033..9563
                     /locus_tag="IVK93_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386339.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            9717..10868
                     /locus_tag="IVK93_001033"
     CDS             9717..10868
                     /locus_tag="IVK93_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6386340.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(10977..13016)
                     /locus_tag="IVK93_001034"
     CDS             complement(10977..13016)
                     /locus_tag="IVK93_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP6386341.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13074..13829)
                     /locus_tag="IVK93_001035"
     CDS             complement(13074..13829)
                     /locus_tag="IVK93_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6386342.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            complement(13921..15051)
                     /locus_tag="IVK93_001036"
     CDS             complement(13921..15051)
                     /locus_tag="IVK93_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6386343.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(15592..15798)
                     /locus_tag="IVK93_001037"
     CDS             complement(15592..15798)
                     /locus_tag="IVK93_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386344.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(15814..16428)
                     /locus_tag="IVK93_001038"
     CDS             complement(15814..16428)
                     /locus_tag="IVK93_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP6386345.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(16575..17258)
                     /locus_tag="IVK93_001039"
     CDS             complement(16575..17258)
                     /locus_tag="IVK93_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6386346.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(17367..17825)
                     /locus_tag="IVK93_001040"
     CDS             complement(17367..17825)
                     /locus_tag="IVK93_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP6386347.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(17909..19672)
                     /locus_tag="IVK93_001041"
     CDS             complement(17909..19672)
                     /locus_tag="IVK93_001041"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP6386348.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(19893..21353)
                     /locus_tag="IVK93_001042"
     CDS             complement(19893..21353)
                     /locus_tag="IVK93_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386349.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(21561..22487)
                     /gene="glsA"
                     /locus_tag="IVK93_001043"
     CDS             complement(21561..22487)
                     /gene="glsA"
                     /locus_tag="IVK93_001043"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP6386350.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(22510..23721)
                     /locus_tag="IVK93_001044"
                     /pseudo
     CDS             complement(22510..23721)
                     /locus_tag="IVK93_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(23756..25369)
                     /locus_tag="IVK93_001045"
     CDS             complement(23756..25369)
                     /locus_tag="IVK93_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP6386351.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(25481..25690)
                     /locus_tag="IVK93_001046"
     CDS             complement(25481..25690)
                     /locus_tag="IVK93_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP6386352.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            25781..25969
                     /locus_tag="IVK93_001047"
     CDS             25781..25969
                     /locus_tag="IVK93_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386353.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            complement(26679..27893)
                     /locus_tag="IVK93_001048"
     CDS             complement(26679..27893)
                     /locus_tag="IVK93_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6386354.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(28021..28491)
                     /locus_tag="IVK93_001049"
     CDS             complement(28021..28491)
                     /locus_tag="IVK93_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6386355.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(28488..28730)
                     /locus_tag="IVK93_001050"
     CDS             complement(28488..28730)
                     /locus_tag="IVK93_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP6386356.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(29276..29806)
                     /locus_tag="IVK93_001051"
     CDS             complement(29276..29806)
                     /locus_tag="IVK93_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6386357.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(29988..30644)
                     /locus_tag="IVK93_001052"
     CDS             complement(29988..30644)
                     /locus_tag="IVK93_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP6386358.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(30771..31244)
                     /gene="dfrF"
                     /locus_tag="IVK93_001053"
     CDS             complement(30771..31244)
                     /gene="dfrF"
                     /locus_tag="IVK93_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP6386359.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(31663..31881)
                     /locus_tag="IVK93_001054"
                     /pseudo
     CDS             complement(31663..31881)
                     /locus_tag="IVK93_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(31912..33126)
                     /locus_tag="IVK93_001055"
     CDS             complement(31912..33126)
                     /locus_tag="IVK93_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP6386360.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(33467..33709)
                     /locus_tag="IVK93_001056"
     CDS             complement(33467..33709)
                     /locus_tag="IVK93_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6386361.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(33741..34298)
                     /locus_tag="IVK93_001057"
     CDS             complement(33741..34298)
                     /locus_tag="IVK93_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6386362.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(34311..34499)
                     /locus_tag="IVK93_001058"
     CDS             complement(34311..34499)
                     /locus_tag="IVK93_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6386363.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(34512..35078)
                     /gene="amaP"
                     /locus_tag="IVK93_001059"
     CDS             complement(34512..35078)
                     /gene="amaP"
                     /locus_tag="IVK93_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6386364.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            36308..36781
                     /locus_tag="IVK93_001060"
     CDS             36308..36781
                     /locus_tag="IVK93_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP6386365.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            36790..37017
                     /locus_tag="IVK93_001061"
     CDS             36790..37017
                     /locus_tag="IVK93_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6386366.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT        11041 a         7398 c         6234 g        12867 t
ORIGIN      
        1 ttctggggct acctctggtt tttctggatt ttcagtttca ttatctgtgt ctggatttgt
       61 ttccggtact gtttggtttc cagaatccgt atctggttct ggagtcacat ccggtttttc
      121 ttctgggtct acatctggtt tttctccagg ggttatttca ggttttgatt cattgtctgg
      181 ctctggttga gtttctgtat cgggttcttc tccaggttct atttccggtt tctgttctcc
      241 gtctggattt tctgtaggcg gtacttctgg cttattttca ttttctggtt tatctgttgg
      301 ttcttcaaca agatctcctc ctgagtcgat agctgaatct tgctcaattg tctgccgctg
      361 actatacggc gttaaatgat gatgatcgtt cggtagtcca tctgatacct tgacagtaaa
      421 aagcgctggt tttaacaagt cctgatttaa tgtaacggtg aaggtaaata tcgcttgctt
      481 gtgttggata gtcaaagtgt tctcgtcata gccaatcgac aaagcgggat caacattctc
      541 aaatgtaaca gctgattgta gtataggtgc taagtctcct gaatagtcaa tttttgcttt
      601 gatggtcgct gtttgtcctt ttcttaattt tgcgactcct gaattgaagg atgaaaattc
      661 atcctgaaag gtaattcgat tgacgttggc ccacttggtt tctgtccatt tggctgtttc
      721 tatctcctct gtgtggactg tctgatttat agtaagcgat aagcttaagg ctgcaaatgt
      781 tgatattaag atggttgaat atctcataac tctctcactc cttgattttt ttgtatgcaa
      841 gaaataagat agtagagatt ctacctatat gtttactgtt tttttgtcta aatttatgaa
      901 aaaaacagta atattttcaa gttttaacaa tttaagtaaa aaggagcctc tttcatgcaa
      961 actcaagaag cgattaaaac gtttctcttg aagaaaaaaa gcaatacaaa attagatatt
     1021 tttcttttcc tatctgaaca tcgtttcttt attactacac aatatttagc agatcatttc
     1081 catatgtctg aatcaaattt tttactatac atcaaagaat tagaacaaga ctttgagcgt
     1141 ctcaatctaa cagaactgca tattgataag caaaaacctt ttttaaagtt gaattttgaa
     1201 ggaattgatc cggcttactg ctattatcgt ctatttggaa gatactgcaa cgaatcagtc
     1261 agttatcaga tcttgacttc tttgttcagc tgtcagacaa attcgattat cagtttaagt
     1321 cagcaaacaa actatagtgc ctcttatttg tatacgaaaa tgaaaaagat taatgcattt
     1381 ttagcattgt acggtttgtc tatctccttt tctaataatg gcagaaagtc tttttctgga
     1441 aaagaggtac aaattcaata tgcagcatta gatatttatt ggaacatctt ttcaagtact
     1501 gctacagaat tttatgatga agatccgcaa gtagtggctt ttatgatgaa tacttttttt
     1561 aaaaaagaag tacttgaccg actaaatagc ggtatgatag ataaattata ttttctatta
     1621 aaaatatgta aagagaattt tcctgcaact tctgtgaaag acgtacaaaa agaattcaca
     1681 gagtatgaat atattgactt ttttattgat ccctctgtag atattttgcg acctaatctg
     1741 ccaatttgtc gcgaacaacg catcttagtg aatattttag ctcgactttc tatcacgaaa
     1801 atcgaatcgg aagaaacaag cttggagcaa tacaaacttt taaaaaaagc aaacgttcct
     1861 catgttcttt attcaagaga aatggtggag tccttttgta ctgtatttga tttgtttata
     1921 cctgaaaaag aaaagattct ttactcattg gcattcgcac gaaataaact ttataatgga
     1981 tttcttaact tcaatcaacc aaatacgccc cttcctactt tcatgcttta tcaagaacat
     2041 agcgtgttga aagaagtcca aaccgccatt gaacaatttt atatggattt cagagaacaa
     2101 aatcaacact tgttaccagt tattttagaa aaggaaaatg tgcaatggat ggttgaagac
     2161 ctagtacatt tgtatgatcg ctacaaaaaa cagccgcgaa ttgtgattgg tgtgaactat
     2221 acgcgtgatt tttatatcag taaagatctg atcgttaaga tagaacaaat tttttcaaat
     2281 gaatcgatca aaatacaaaa tgattttatg gagaactgca acatcgttat cagcgactgc
     2341 cctttgaaac aattgccttc tcatatcaaa aaaatctata ttttaaaagg aatcatcaca
     2401 ccagaagact ggaaaaaagt gattatgagg atctcagaat atatctttga attaaaagaa
     2461 gaagcactca tggatcaatc gaaaaaagaa aatcaagtta cttcctagac caaataagat
     2521 acataaatcc gaatgcttat taacaacctc cggatttatg tatcaagaat ttgaacatcc
     2581 ttcttattta cataataaaa ttacagtttt tttcactaat tatctttact tttgtttcct
     2641 ttttcgattg cttcccataa tccatttaga taaacagcac ctaatgctcg atcatataag
     2701 ccgtatcctg gtcttcctgt ctcattccag atcattcttc catgatcagg acggatgtat
     2761 ccgttaaatc cgttgtcata cattgttttt agtatgcggt acatgtctaa tgaaccgtat
     2821 tcggatttat gcggtgcctc atagaaatct cgattagcta aaaatttaat atttcgaaca
     2881 tgtgcaaatg ggattcgatc tctttgacaa aattctgcta aaatcccata tacatcattt
     2941 tctggatctt ctgcaatact ccctgtacaa agagtgattc cgttataagg gctgtctact
     3001 atgcggcaca accaatctaa atcttctcga ttctttacga ttcgaggtaa tccaaaaatc
     3061 gagtatggcg gatcatctgg atgtacagcc attttgaccc ctacctcttc acatgttggg
     3121 atgattgatt ggagaaaata ggcaaaattt tctcgaagtt tttcttcgtc tactgatgca
     3181 taggcttcaa gcaaggattt gatatgagct aagcgctcag gttcccatcc cggcaattca
     3241 aattcattgc tggaatgttc gatgcgcgcg acgatttctc ttggatcatc tggaatatca
     3301 tcagatataa aagctaacgt ggatgagccg tcttctaagc ggtagtcaag gtcagatttc
     3361 acccagtcaa atacaggcat aaaattatag cagatgactt ctattccata ttctgctaag
     3421 tttcggattg tctgcttata gttttcgata tactgctcac gggaaggtaa tccaatttta
     3481 atggcatcat gcacattgac gctttcaatt acttttagtg ttaacccacg agactcaatt
     3541 tcttctttta gttctttgat cctctcccta ggccatacct ctcctactgg tatatccatc
     3601 aatgtgccta caatcccttt gaccgcagga atctgtctga tttcatctaa ggtaatcttt
     3661 tcttcctgtg atccgtacca tctaaaagtc atttccatga taaatttcct ccatgttctt
     3721 gtaagtaatc ctgtaatgtt tttcgtacgg cattcggtcc tgctaccatc ttgatgaaca
     3781 tttcttctat ttttctgcca agctctgctt ggtaaagatc tacaccaaaa atttctttat
     3841 ttgacaaaat aggagccatt ttctcatgaa tatgtgatgt atcttgcgta cccagttgaa
     3901 tatcggaaag cttatgctga aagtctgtca atagtgggtc tggactcggg atgaatggtt
     3961 ttccttgatc atcaatgcca ataaggtatc gaagccaagc agcgagagtc aatggtatga
     4021 atacaagtgt ctgcgggtct ttaacttcta tttccatata ttttttaatc gtctcaccat
     4081 aacgaatcgc caccttttga gaggtatccg tggcgattcg ctgaggcata tcaggaatat
     4141 ttggattaga aaagcgcaca gtaattactt cttccagaaa gatctttgga tcaatgatac
     4201 ctggagactc cacaactggg aggccttcat tgtacccgat ttcagaaact agcagcgata
     4261 aatctggatc tttcatctcc tcagctatgc tcctataccc cagtaaacaa cccataatag
     4321 ctaaagacgt atgcaaagga ttaaggcaag tcgtcacctt catcgtatct gctttatcta
     4381 ccgttgcacg atcagtcaag aatactccag ctttttcgaa tttcggacgt ccattgggaa
     4441 aatgatcttc catgattaaa taatggatgt cctctgtatt gacaaatgga gcaatattat
     4501 ttccttttgc ggtttggaaa atcgacatat ttgaaaagcc ctcttttgcc aacattttgc
     4561 taactgacaa ggaaggattc ggggtgatac gatcaatcat cgtccatgga aacgcaacaa
     4621 tttcttcatt cgataagtat tggataaatg tattttgtac ataaccttcc ttttgccatt
     4681 cttttgcgat cgttagcaaa gaagtacgta attgactacc gttttgtgaa aaattatctg
     4741 tagagacaag tgcaatcggc caagctccag ataaaaaacg tttataaagt agcgcagcaa
     4801 gaatactcat tgtatgtgat ggcttctttg gaccattttt tatatcgact gcaatcggtc
     4861 ctatatattt attatttgta tctttcaatc gataaccttt ttcagtaata gcaaaagtaa
     4921 ctaattgtaa actaggattt tcaaagtgtc gaatcatctt atcaaagtgt tcagaacaat
     4981 ctggatgaca atacagactg tcagctgtcg cagctaatat acgtttgacg aactctccac
     5041 tgcttggcat gaccacttca agaatcaaat catcaaaatc ttggtaagcc ctttcaatta
     5101 tttcttcatc gaatgtttca caaacaatga caccagtgga caattcgcct agatccgcta
     5161 aacgttgagc gatttctgca tgaaatcctc gataaagatt tccgccaccg aaatggatcc
     5221 agacaggatt tttttgtcca ttttctctta atgatgcacg gtcaaaatga ggtatttcta
     5281 cttctttttg agataattca gggataaagt tcctccagtt gtttaaatca atcatttttc
     5341 ccctcctaaa ctaacatatt agtttttgta ttcaagtgaa ttttatgcaa taaaaatcgc
     5401 tatgtcaacc aaaattttac tatttataca aatttactag ttaatgtgac ataccagatt
     5461 gtttttcaga cctcttctgt tatactacgt tcatagaaat gattttggag gaataaagat
     5521 gaaaagcagc ttgcaatctc aagcttatca agcgattcgt aaacaaatta tctatgctga
     5581 cttagaacct ggaaggaaaa tatctgaaaa ggcactggag gaacatcttg ctatcggccg
     5641 gactcccatt cgcgaagcat tgattcaact acgtcagcaa gaactcgtgt atacgatacc
     5701 tcaaagtggt acgtatgttt caaaaattaa tcttgtttct gcagaaaatg ctcgctttgt
     5761 ccgagaacag ttggaagagc aaatcatgat tgaatgttgt tccaaattgg acacacaaac
     5821 acgaatagtg ctagaaacga ttttagaaga acaagaaaaa gcagtcaaaa taaatgacga
     5881 acgcgctttt ttccgtgcag ataatttatt tcatgaagct tgtttcgaaa ttgcaggaag
     5941 aaaagaagtt tggaactggt tagatgacca taatacccat ttagaacgtt tccgctggtt
     6001 acgagtgacc attcaggggc ttccatggga aacgatcatg gaacaacatt atgatttact
     6061 caaggcttta gttgataaaa aaccagaaga agctcgattt caaacgggat tgcacttaca
     6121 catgatgctg aaagaacagg aaactgttac tcaacgttat cccgagtatt tccagcagta
     6181 gcattttctt gcgacctctt ctatttttat aattaacctt cgtttttgag taatctttat
     6241 taaataacaa aatatccatt gactttgcaa ctccaactaa attacaataa ttataaataa
     6301 ataattatta ttggaggttt tactatgtca ttgattggga aaaaaatcga agaatttaat
     6361 gcacatgctt atcatcaagg agaattttta gaagtcagtg aaaaagatct tatggggaac
     6421 tggagtattc tttgcttcta tccagctgat tttacttttg tttgtcctac tgaattagaa
     6481 gacttacaag aacaatacag tacattgaaa tctttaggtg ttgaagtctt ttcttgctct
     6541 accgataccc actttactca caaagcatgg catgatacat cagatgctat cggtaaaatc
     6601 gaatacgtga tgatcggtga tccgtctcat cagatttctc gtatttttga tgttttggat
     6661 gaagagcaag gattagctca gcgaggaaca tttattattg atccagacgg aatcgttcaa
     6721 gctatggaaa tcaatgcaga tggaatcggt cgtgacgcta gtgcactgat tgataagatt
     6781 cgcgctgctc aatatgtacg tacccaccca ggtgaagttt gtccggcaaa atggaaagaa
     6841 agtggcgaaa cattgaaacc aagttttgat cttgttggaa agatttaggt ggtggatatg
     6901 ttagacaaag aaactaaaca aaatctcgaa caatatttag cactgattga atctcctatt
     6961 gttttttctg ttagtttgga taatagtgaa aatagtcaaa aactagctga atttacgaag
     7021 gaaatagcag aaatgtctcc taaaatctct tgggaaaaaa gagattcgat gcgcacacct
     7081 agtttttcta tcaatccagt tggcaaagaa agtggaatcg tttttgctgg tatccccctc
     7141 ggtcatgaat tttcctcttt tttactggct atgttacaaa ttagtggacg ggcaccgaaa
     7201 atcagtgaat ctttgagtct taaaatcaaa aaaataacac aaacattgca ttttgacagt
     7261 tatgttagct tgacttgtca taattgcccc gacgttgtac aggcattgaa tatattagct
     7321 gtattaaatc caaatattac tcatacgatg attgaaggtg ggatgttcca aaaagaaatc
     7381 gatgataaaa aaatcatggc agtgccaact gtgtttttaa atggagaaga atttagtagc
     7441 ggacgcatga cgatcgaaca aattgttgaa aaaatcactg gccctttgat tgaagaagag
     7501 ttgtttgaaa aagaacctta tgatgtatta gtgattggcg gtggaccagc agctagtagt
     7561 gcagctatct atgcagcacg taaaggaatc cgtacaggac ttgtcgctga ttctttcggc
     7621 ggacaagtcg ttgaaacact agggattgaa aatatgattg gtacctctta cacagaaggt
     7681 ccaaaattga tgcagcaagt agaaagtcat gtgcgtagtt accctatcga cattatgatg
     7741 aatcaacagg ccgtttcctt aaataaagag caacatttta ttaatattgg cttagctaac
     7801 ggtatatcac ttaaagcaaa aaccgccatc atcgcgacag gtgcgcactg gcgttctatc
     7861 aatgtaccag gcgaaaaaga gtttaaaaat aaaggaattg cttattgccc acactgtgac
     7921 ggccctttat ttaaaggaaa agagattgct gttatcggtg gtggtaattc tggtgttgaa
     7981 gcagcaatcg atttggctgg gttagcaaaa catgtatatg ttctagaatt cttaccagaa
     8041 ttaaaagcag atcaaatctt acaggataaa ctagtctctc ttgcgaatgt cactgttatc
     8101 aaacatgcag cgacgaaaga aatcagcggt acgaatcagg tagaatctct ctcctatatc
     8161 gatcgccgta cgatggagat gcatacacta gctgtttcag gagtattcat tctagtcggc
     8221 ttactcccta atacggattg gctagatgga acgatcgaaa tgaatccaag aaatgaaata
     8281 gtgaccgaga aaaatggcgc aacgaatatc cccggcgttt tcgcagctgg tgattgtaca
     8341 gatagtccat acaaacaaat cattgtttca atgggttcag gagctactgc agctttagga
     8401 gcattcgatt atctgatgcg cacttgagct atgaagattg taaaaagtca gagagcggaa
     8461 aaaaggatgg tgacaagaat tttgtcacaa tcctttttgt ttttttgaat aatcgctttt
     8521 tcttctcttt ttcttatata ctataagtag taaataattt gaaaaaaaga aaggtgactg
     8581 attatggcgc aaaatcgtat ttttacgaca agttttgcaa gcgtttatcc tatgtatgta
     8641 gcaaaagcgg agagaaaaga tcgtataaaa gaggaagtag atgaaatcat ttgttggctg
     8701 acgggttatt cccaaacaga actagaatat caactacaaa aacaagtgac tttcgaacaa
     8761 ttttttgcgg aagcccctgt ccccaatcct tcacgttcct tgattacagg tgttgtttgt
     8821 ggtgtacgag tagaagacat cgaggataac ttgatgaaag aaattcgtta tttagataaa
     8881 atgatcgatg agctagcaaa aggcaaaaaa atggaaaaga tcctcagaag ttgatttttc
     8941 cagagatatt cttctccttc gctcattttg taacatttaa tttggtaaaa tgactagttc
     9001 acgttaaaat aagtcaaagg aggttttctt atatgtttga gacgattaaa caaaatattc
     9061 aaaaatacgc cttattaaga gctgctgttt atattatttc tgggattgcg attgtgctaa
     9121 acccttctgc tgtttttcat tttatcggtt atctgatcac cgcctatctt gttttgcttg
     9181 ggttgttgaa tctttttgag gcttaccgtt ttaaaaggaa aacaggttct tggagtatcg
     9241 gtctgttcag tggtcttttt ttcctgatct tagctctgat cgtctatgct tttgcacctg
     9301 caatcgtttc tatcctgccg attcttcttg gattagctgt tatcatcaac agcttgttcc
     9361 agttgttctt cagcatgaat acaaaagcta aaagttggag tgtttatagt agtattctgt
     9421 tgttgattgg cggattcgtc ttattgttca atccattcaa aagtttgatg cttctattcc
     9481 aaatattcgg ctttattttg attttcatgg gcatccagga gattatcaat ttcatcagaa
     9541 taagaaaaat gactatacgt taatcatttt tagttattaa aatagaccgt ataaaaacca
     9601 acaatccaaa aaaatctttt ggattgttgg tttttttgtg agtatatttt cctatttttt
     9661 caataatgta tgttatgcta agttcgggct atattttata caaaggaatg atgagaatgg
     9721 aatttattgg tattttatgc ctgatattga tttcaacaac gattggctca catatctcgc
     9781 ggagattagg tatcccagca gtgattggac aattacttgt tggtgttttg ctaggccaag
     9841 ctggattgaa ttgggtcaaa cctgatattc tagtccatga cttttcggag attggtgtga
     9901 tcttattaat gtttttagct ggtctggaaa gtgatttatc tcttttgaaa aaatatttcc
     9961 gacctggtat gttcgttgct ttgcttggga tcatcttccc ggttgttttt ggctggttga
    10021 caggcaaagc attccaagtt tcgggaaatg aagcgatttt ctttgggatt attcttgcgg
    10081 caacttctgt cagtatttca gtagaagtat taaaagaact aaacgtagtg aatacaaaag
    10141 aaggatcaac aattttgggt gcttctgtgg tagatgacat tttagtcgtt ttagttctaa
    10201 gtttcagcct atcttttcta acgggggaag cttctagcaa tgcttcttta ccgcttgttt
    10261 tgattgaaca gctaatttat tttgttttta ttttcttatt agtcaaatgg attgcgcctt
    10321 tcctactttc atttgcagaa aaagtgtacg caaattcttc tattatcatc gtatcgctag
    10381 ttatttgttt aggaatgtct tacgtagcgg atcttgttgg gcttagctct gtcattggtg
    10441 ctttttttgc tgggatagct gtcagtcaga caaaggttaa agaagaagta taccacaatg
    10501 tagaggcttt aggatatgca gtatttatac cagtattttt tgtcagtgta ggattagaag
    10561 ttgatttttc tgactttagt gaacaactac tctttatttc tatattgacg cttgtagcga
    10621 ttttgaccaa attgcttgga ggttattttg gtgctcagat tgccggtttt tctcaacata
    10681 gttcattgat ggtcggtgct ggtatgattt cgcgtggaga aatggctttg atcattttac
    10741 aaattggaca gcaaagtcaa ttgatcgaac cacagtacta ttcaccttta gttatcgtga
    10801 ttttattaag tacacttatt tctccacttg ttctcaaata ttttacaaaa aaagtttatg
    10861 aagtgtgagt aaatcaaaaa gaagttgtga aagagacatc gtgaaaaccg tttatttgaa
    10921 aaaaggactg tgacgataac gccacagtcc ttttactgta cttaaaaagc aattttttat
    10981 tgataatttt ggttgaggta ttgcaatagt tccgatgctc gtttggttgc tgaatcatca
    11041 tcttctttat tttccaacat gctgaccatc atataacctt ggttatcaag attgaaagca
    11101 aacaagaaac tgttctcttg cccctttaca tcctgttttt ctttgatttc cgctgtacca
    11161 gttttcgctg ccaacggaat ccctaaagca gaaagagaat gtgctgtacc atttacatct
    11221 tgcacaactt ctctcagatc tggcacgatc gtttgtactg ctgtttcgct gatgacattc
    11281 ttcttatctt ttgtctcttt atctgcaatc aatttaggat agacaagtgt gccattgttg
    11341 gtaaaaacag aatacattgc tgcttgctgg ataggagtaa ttagaagttc gccctgtcca
    11401 tatccagtat cagctagcaa gatatctgag ttaaagctat cttcattaga aatttgtgct
    11461 ggattcatac taattggcaa atcaaggtct tcaccaaaaa tgaatttatc taagcctgta
    11521 cgaaattttt tctcacccat tttcaacgtt tcttgtgccg catatatatt atcggaatag
    11581 atcaaagcag tttttaagtc tacttgtgat acatcactac tgacacgcgt tgcttgataa
    11641 gatccccaag aactgtcttt ttgccattta agcccgttga tcgtcaacac ttcatttgga
    11701 tcgatagtgc cgttgtcgag accgattgct gctgtaatca ttttaaacgt agatccagga
    11761 gcataacctg tcgcaaatcg gctgatgaat ggatgttcag gattttcttc ataagctttg
    11821 taatcttctt gtgagatccc gtttgtcatt ttgtttggat catagcttgg agagctagca
    11881 agcgcaagaa gatcaccggt ttttggcgtt gtcgcaacag ttgatccagc ttttcctcct
    11941 agactgtcaa aagctgtttt ttgtgccttc gcatcgattg tcaatttgat atctttgcca
    12001 ttttgaactt catgttcgat cagaactttt ttctctacac cgtctgcatc tgtaatgctt
    12061 aatttcccac ctgttgtccc acgaagatcc ttatcaaaag ccatctccaa acctgagcga
    12121 ccgatttttc cattactgct taattctgga ttcttatcaa tatcttctgc tgtgatatct
    12181 cccacgtaac caatcagctg agcagctgct tcacccaaag gataatatct gccgtctact
    12241 tcttgaatgg tagctccagc tggaagttct ggcgttgctc catcaatgat tttcaatggg
    12301 acaaagtaat cgggttgtac ccagctttgt gaaatcgcct gattgatagc atcttctgtt
    12361 aagtcgaagg aggaagcaat cgctttgata ttggctgttt tttcgcctcc atctccaagt
    12421 ttacttggaa cgactcctaa ttgttttagt ttgcctgttg ttgctaatgg ttcaccattt
    12481 cgatctataa tattccctct tgctgcttct tgtgtggtca ggcttacttt gtcatttcct
    12541 tccatttctg gaaaaaccaa gttgggttgc cagttgatcg tggtttgacc atcatttctg
    12601 tctaatgttc ctttataaga aagatctttc aattcaccta atgaggtatt catctttgct
    12661 ttatatgaaa aactgtaagt agaatcgtct tttttatcta cttttagatt cgatatctga
    12721 agtcctttga catcagcagc accgtatata ttctggtatt tatctaagat ctctttttca
    12781 gataatgcac ttttatttgc cgcctttttt gatgtcattt ctgcagcttt gttataatct
    12841 cccttgttca aagcttggac aaattgctca actgtctttt ctccagcttc tactgcttgg
    12901 gtttcttgat agtgctggta gtaaaaataa ccgccacccg cagctgctat taagatcact
    12961 gcgccggcaa tataagcgcc tgttcgattt ttgccgtgct tgtcacttct tttcattttt
    13021 tcctccttct cttgcttgtc attcctttat ttcactattt atacctgttt attctaacat
    13081 atgtctttga ggaaatccat accacaatcc agaagcacac tttctggaag gttcgtttgg
    13141 atactaccaa gtaatttccc aaccgctttg ggtagcttga atattgaagt tacgtctttc
    13201 agctgctgag agatagcttt gatcacttgc tgctgccttc tgatcctccc gaagtctcct
    13261 tctttgtctt cccgaaatcg agcatactgg agtaaagtat taccatccat cacttgctct
    13321 cctttgttta tatatacgcc atctaaatct agagattttt ctgcatcgat cttgactcca
    13381 ccaggaaaca gttcattaac gcaatcgata aaatcttgaa atgtgatact tgcataatag
    13441 ggagcttcaa atttgaaatt ttcctttaat gtttgattta ataaatcaac tcccccataa
    13501 gagtaagctg catttatctt attcattccg taacctggaa tatcgacata tgaatctctc
    13561 atgatagaga tcagttttgc ttgttgtgtt ttctggttgt attgtgcaac cagaagtgaa
    13621 tccgagcgac cgttttcgtc tttcgaacga gcatctgaac caatcaaagc cacatttagt
    13681 gtggtaggga tacttttcgg ctctaccttt ttcacagttg aactctgttt tgcagacgaa
    13741 aatccatgga taaactcaat cgaaaaatag ccaactacta atgtaagcag taacaaaata
    13801 gataatatga ttttttgaga tactttcatt gcgaattcct ctttctaaaa gaacctctcg
    13861 cttgaaaaat aaaagattgt actttatgat aattcaaact gagagtttct cagtttctct
    13921 ttattgtttt tcaggtcgat agagcgccaa ctcttcggcc tgtatctttg aagaaatatt
    13981 caacgtgatt ccgattccta aagaaaggat cagataactt gttcctccgt agctaacaaa
    14041 tggcaatggc acaccagtca ttggcatcag ccccgcgata cttcccacgt tcattatcgt
    14101 ctgaacgaat agcaaagttc cgaatcctaa aagaaacaat ccagcttgct gatttttaca
    14161 gcgactgctt aaacaaaaga ttctcatgca taatgagaac aataagaaaa gcacacacaa
    14221 tgcaccgatt agcccaagct cttctgtaat aatcgaaaaa ataaaatctg tttctgtctc
    14281 tggaagatac cctttttttg tgatactatt tcctaatccc cgcccaaata tgccaccatt
    14341 atatagtgca taaaacgaat gggtcatttg atagcccgca ccatgactat cgataaaaga
    14401 atctcttaaa gtcgcgatcc tttcataagc atgattaaac atctgtggaa gccaaccttt
    14461 atttcctaaa tataataccc cgcctgctgc tccaatcagc aatgcagaaa aagtaacgat
    14521 tagatagatc ccttttttaa atggaaccgc cgctgcccaa aatatgaccc acgcaatcga
    14581 aaggatcatc aatgctccag caatctttgg ctgaaataaa atcagaaaag ttatacctac
    14641 tgtaatcagg aatggttttt ttagatcttt tgggctacta tttccgtcac gaaaaaacca
    14701 gcttaaataa aaaatcaaaa aaagatttgc cagttcagaa ggctggaatt gaatgccaaa
    14761 cagagagatc caacgttgtg cgccgttgac agtgacaccg aatatcccta ttcttactaa
    14821 tactaaaaag aaaatcgata aggctaaacc gtatcctgct attttagggt gaagtaaata
    14881 gtgtagtttg actgaacgag ctaagaagat cacgctccaa ctcaaaaata tgaaaattag
    14941 ttgacgcaat aatagacttt ttgtattttc atcggcttgc aataaacgat aagaactggc
    15001 actgtatact tctaaaagac cgatcatgga caaagtcaaa taaggaccca aaatccacca
    15061 atcgatcttt tttcaccgca tctcctcctc acacagcaat ccacttgtca ggtgaggagc
    15121 gagtttgtct catcaaataa gtcagtcaca aaaatataaa gaaaaggtca gaaacctttt
    15181 cgaacaagaa ttctgaccta gcatcatctc atcgtccgag ttttagcact atacaacgta
    15241 aagaaaacat tcttagctat tggaaaaagc cttaatccaa cagtttctgt tttacccatt
    15301 ggcgactttc gacgtttctg ggcaacagcc tatgtttgta taggagcctc acctaacaag
    15361 gttattacta tattctattt acgagttcat gatagacttt tcctaatgat taggcaatgg
    15421 aatttgaaat gaaatagtga aatttaacaa tctttaatat tttttagaaa aaaataacaa
    15481 ataagttgaa agaatcccgt tgcaaattct ttcagttgca ccaggattct ttgataattt
    15541 tttagtttta tgtgtttttt tatatgtttt ttcacttttc ggaataaata tttatttgtt
    15601 aaagtaatta aacaatccgc taatcgtttg atctggcatt tctatttcct taccatctaa
    15661 agtaacagta gtaacgacaa aatggtcttg caatggcgga acttctaagt actgcccttg
    15721 cagaaaatca ttcactgttt catattcttt tttcatttct tttgaatctg tcgatggttt
    15781 atagcttatt tttaacataa ctagtgctcc tttttattcg ggacgtgcaa tcgtgaaaat
    15841 ttctcctatt gctgcataat catttcctgc caaacggctt acagcattca attttcttgg
    15901 atcgatcctt cctgtcttat catcatagac tgtttcattt aaatgatatt tctgtacttc
    15961 tagcaaaaat aaatcagctg ttactttatt tttattccgt atctcgacat gttcatataa
    16021 ggatgtttca aaacgaactt ttgcttctgt caatgctggt actgaaatga ttactgaatc
    16081 agttaaagtc aagtcagtca ggtacagttc gctttcctca ttaggcagat tggctgctgt
    16141 tttatttact tcttctacgt tgtcctcatc aaggatatgg ataacagctt ctcttgtctc
    16201 aagaatattc tttgctgtgt cttttactgc atcttgtttt ctctggatcg ccaaggataa
    16261 gatgggtcga ttcgaactga cgatattgaa gtaactgaag ggtgcgatat tcactatatc
    16321 ttttgctgat ttagtcgtta ctactgctac tggtctaggg atgactgaac caatcagcaa
    16381 cttgtaattt tcacgttctg tcaattctga aggactccgt tcaaacatgg tttcgccttc
    16441 tttctataaa attcgttaaa aagcagatct tgaagacaaa atgaaaagta atgctttttt
    16501 catcttctta gaatctgctt taaactatct atcaattttt cttttttgtc aagaatctgt
    16561 gccgtgcgtg ttttttagtt atttgcttta cttgcggctt cgacatatct tttagtgatc
    16621 aattgcggac gggtgataat agatccaact acgactgaaa aagcacctaa ttcatatgct
    16681 ttctctactt gttcaggaga gtcagtatgt ccttccataa ttactgggat atcgacttgt
    16741 tctatgattt gattcaataa atcaaaatca ggtgtctgcg tctttcgtga atattctgta
    16801 tagcctgcta aagtcgtaga caaaagatca aaaccatctt cttgcgcttg aagcgcttcc
    16861 tcaaaagtcg aaatatctgc catagctaac cgaccgtgtt cgtgaatcat ttggacaagt
    16921 tcagcagttg ttttttctgt cggacgttta cgagaagttg catccagtgc aatgatttct
    16981 gcttgcgtct ccaaaagttc ttccacttct ttttctgtcg cagtaataaa cacttctgac
    17041 ccatcgtaat ctcttttgat aatccctatt accggtaaat caacgattcg cttgatttca
    17101 ttgatatctt ctgtcgtatt tgctcgaata ccaactgctc cgccttcttt tgcagccaaa
    17161 gccattctcc ccataataaa agaactgtgt agcggctcgt tttccaaagc ttgacaagaa
    17221 acgacgactc cgcctttgat ttgttccagt gtatccacaa tatctcctcc agtaatgtta
    17281 tataaaaata cagcttcata taaaaatgta acccttttct attcaggaga caataatgag
    17341 ctatttcttt cagcagacct ttcttactaa aagaaaatta cttcaaattc ttcgtatacg
    17401 accagcttct cttctgtaaa ttctatgttc aacgcttctt tataagcatg tttaaaaaac
    17461 tcagcatgga ctttccgcca ataagctaat gtccgatccc cttctccttc tttccaagca
    17521 tgctctttcg tgacttcact aaaaggcaag atggtcacat ttatattttt gatgatacaa
    17581 actggttcat tttttccatt caggaccacg tctaattgac caacttttgg taaaggctca
    17641 ttctcttgtt tatacagatc gtaaccactt gatgtaccag tctttatctt ttttacaact
    17701 agttcgctca actcgtctgc cattttttct gtatccccaa atgaaaagaa acctaatttt
    17761 tctgattgat aactaggata tttttcttgg aactttttga catattcttc tacttgctga
    17821 tacataacat ccaccccttt gctttttata atgaaaaaac catccagata ctagtataaa
    17881 caattatctg gatggtgagc ataatgaatt attgaatatt caagacacgc agactatctt
    17941 caacgatttg ttttggtgtt aaatgataac gttcataaag ctgccctaca ggaacgctat
    18001 ccgtgaattc tttctctgca ccaaaattta atacgttcat tgaagaatca ccataataac
    18061 gactgatatt ttcgccaaat cctcctgaca aactaccatc ttctaatgtg atgactaatt
    18121 cgtgtgtttc ttttaattta tctaatgttt ctacgtcatt ttctgtgatc gttcctggat
    18181 tgatcaacgt tgcttcgatt cctttttttc taaaagcttt tactgtttct tttcctaatt
    18241 caagaaaacc gcctaaagct aaaattgcta cttttcctcc attatttact gtttgatagg
    18301 ctggaatact ataatcattt gcgatagctg taccatgttc tacaccatgt gtaggaattc
    18361 ggattgctac aggctgcgct gtttgttcga acgcccaatc tagcatagag atcatatcct
    18421 cttttgttgc tggagcaaga tatttgatgt tcgggatact tgataagtaa ggaatgtcaa
    18481 atgttccttg gtgtgttcgg tcactagctg agatcgttcc gccaccgact aaaataatca
    18541 ctggattctc gttgatcgct aaatcatgtg ataattgatc atatgcccgt tgcaagaaag
    18601 tacttgattg gaagacgact ggacgtactc cctgagaagc caaagcagaa gcgaaagtga
    18661 tagagtgctg ttcagcgatc cccacgtcaa aataatgatc tggatgtttt tcttggaatg
    18721 ttttcagtcc aaaggcgcct ggtatcgctg cattgactgc ggcaatagga gctgtacttg
    18781 tttgcatttt cttatccaaa taatccatga tgatcatatt aaatgtttct ccagaagtta
    18841 cttgctttgc ctccccggtg gacagatcaa atggtacatg ccaatgatat ttcattttat
    18901 tatcgattgc cggctgataa ccgcgacctt tttccgtatg gacgtgaagt acaatcggat
    18961 gatcaatatc ttttacttct tcaaataaac ggatcaccgt ttccagatcg tttccattat
    19021 ccacgtaacg ataatcaaat cccattgctt taaataggtt attgctgctt ttaccgttgg
    19081 tttgacgaag ctcagctaag tttttatata aactgccatg attctcatca atagacatct
    19141 cattatcgtt tactaatacg attagattgg attgtaaaac accaccattg ttcaatcctt
    19201 caaaagctaa gccaccactt agggagccgt caccgataaa tgctaccaca tttcctgatt
    19261 ctttcttcag atctctcgct ttagctaaac ctgttgctaa gctgatagat gtagatgtgt
    19321 gccccactgt aaaatagtca tgtttacttt catgttggtt ggaatatccg ctcacttcac
    19381 ggtaatgtcc tttttcaaaa ccattttttc gaccagttaa aagcttatga ccataagact
    19441 gatgggaaac atcccaaacg attttatcct ttggtgaatc aaatacataa tgaaacgcaa
    19501 tcgttaattc tactacacct agattcggac caacgtgccc acctacatcg ctgtcctttt
    19561 gcaagatttt ttggcgcatc tcttctgcca actgtttcat ttcctcaaca gataattttt
    19621 tgacatctgc ggggctattg attgcatcca atacatgtaa tactgtactc ataatacatt
    19681 ctctcctccg tactatcttc ttttaatgaa aatagatttg ttacacttta tctattctac
    19741 tacttagagt tgactctaag tcaatagcat tttaaaaaaa tttcagagaa agcttgttat
    19801 ataagtagat cttcgcacga atcaggactc aaataaaaac cttctacaga caattaaaaa
    19861 gctttgtaga aggtctccta attaaggtct catcaagaaa tatgtttttt taataggtga
    19921 agttcctgag caatatgata caaatggatg tttgcacctt ttaatgagat tggatagatc
    19981 tttgtctttt cagtttctaa tccattttga tagctagcag catcgcacaa aaccactctt
    20041 tttttcgcac atagacattc atcaggcaat tcttctaatg aacgaaataa aataggattt
    20101 atttcagcga tgccttcttc cgcatacaaa tgaaattggg ttcgataaaa aaattgtttg
    20161 actgaacttt ctgaaacaac aaaaatttct atttcctgtc tccccgcttg ttcactgttg
    20221 atcaaagaaa gacttttgac gaattttcga atgaaatttt cattccatct tagttggatt
    20281 tgatttttct ttttccactc ttccaatatt tctacaattt ttccgtacag aagttgttct
    20341 ttttctgata gaagatagat cctttccggt aaaaatactt gtccatctgc ccacaacgta
    20401 cggacaaatt gtaaaaagga ttgcttgaaa ataaaatttt ctgcatcgtc gatttcagcg
    20461 atactaataa tctgtaaaat caattcattc agtgataggt cattgagaac aaaattttta
    20521 taaacaactt cgaaatcttt tcttaacagc tccatgttct cattggtata atttctactg
    20581 ttgaacaaag aaaaaatata atagacatct tcttcttgta agtctagaat tcctttttgc
    20641 tcttccaatc cttgacagac taattgaaat aacggcttcg tttctgcgct tttcttatct
    20701 tcttctttca gtccaagggg aaatgccttt cttccgattg cgatctctat ttctcgtatg
    20761 ataaaggact tttcatcttc cgaatatctt cttttaaaaa aatgagtctc tacataatca
    20821 atcaactcgt tacaagcttc ttctacatac ggatttttat tttcatagcc ttcccaattg
    20881 acataacgta ctaaagctag caagatgttc cgcgagtcaa attctgggat aatgatttct
    20941 ttatttttca aataatcatt ttctttaaaa aaatccaata cgattttttt gattgtatat
    21001 actttactca aagaaataaa ttcaagatca gccagttctg tactcgtgaa tataccatta
    21061 aaataataac ttaaaaactt taaaaaataa gattgaccgt acagaaactg tgtgagctca
    21121 gaaagagcaa taccatgttt gaatacgagt ttatacttat gcttctctct tacttgttct
    21181 tttatacaat attctagaca ctcggctaga ctttctagat cgtgaaggat cgttggtgta
    21241 gtcaccctta gagaaatagc tgtttgttcg atgtctattt cttggaattc aaataattgt
    21301 tcacacagat acacttttct aaatatattt cgctcgatgt aattttccag cattcctgat
    21361 acacgcctcc agtttttctt atgattttat ttaatcatat ctgtaaatcg tatttttgtt
    21421 tcctttttat ttagaaaagc cctttcaaaa accaataaaa aaagaaactg ggacaaaagt
    21481 caaacaagct cacaatttca gcctcattgt aggtagcttt gagccgtttg gacttttacc
    21541 ccagcctctt tcaagaatgg ttattcaaag atgtcagcgt ctagttcttt gactacatct
    21601 ttcaacaatt ggataccagc tgcgctgtta ccaaatggat ctagtgccgg gctgaagaca
    21661 cctatgccgt aacgatttgg cacagctgcc atcaacccgc cgcctacacc gctttttgca
    21721 ggtacaccaa catggacgga aaactctcct gattcatcat acaaaccagc agttgtcata
    21781 attgatttga cgattgtagc actttcttca gtaatgattt gtttgccatt ccaaggagca
    21841 attcctttat ttgccagcac agcagctaat tttgctatgc ctgtagcggt gacattgagg
    21901 gagcattgtc tgaaataagc atcgagtatc tccggtacgt ctccttcgat catttgatta
    21961 cctttcatat agtaagcaag agagcgattg atatcacctg ttcttgattc agaatgatag
    22021 atttcttcat tcaatgtgat attaggatca tcacagatct ctttcataaa tgacaagatt
    22081 cgctcaaatt tttcatctgc atctttaccg gcaatcaacg aggttgttgc aatcgctcct
    22141 gcattaacaa aaggattcat tggatgtttg cgatgattga tttccatatt catgatagaa
    22201 ttgaaggcaa aaccggttgg ttccgtgttg attgtttgaa agactttttc tattccgttg
    22261 tctttgattg ctaataacaa tacgggaact tttgacatgc tttcgatggc aaagcgcaca
    22321 tcggcgtctc ctccctcaat cagttgtttt gtatccaaat cataaatcgc catacctaat
    22381 tgtttgggat ccacttcagc taatgctgga atgtaatccg ccacttgtcc tgtttgataa
    22441 tagtggaaat tttttccaat caattgttct aattctttta cttccatttg aataaccagc
    22501 tttcattttt tatttttctg catgctggaa tttatgacgg catgcgtaga tcgtaaatgg
    22561 aataactaag acgataagat aagaaacaat cagaatcgtt tcataaatcc catgctgtcc
    22621 agctgcgata ctagcaggtg gaacaaatga gatacaaaaa gcaaaaagtg aagtcaaaag
    22681 acctaaacaa gcaacgatta cttttacaac ttttccgcct ggaacctgat aggctctttc
    22741 aagactgtca tctttaaaaa ccagcttcaa atagcccgcg aaaaacataa agtacaacat
    22801 caggtagatt acaacagtca atgacatcgc tgcaagaaat gaaagattat tccccccgcc
    22861 gcttaatgta aagacggctg cccagattgt tgcaacgatc ccatttgcga tcatcaaatg
    22921 agcaggaaca ccgtgtgtgt tttcttttgc aaaatattca ggaagtaatc cttcttctgc
    22981 tgctacgtga agtgcttttg aaggagaggt tacccaagaa gcaatttctc cgatgacacc
    23041 taaacaaaca agaatcgcaa gaattttcgc aatccatgca agatttggat caatgtgtct
    23101 taataggaat tctaacgatt gaatcacccc tgtattaaga gacaaaccat gttgaggtac
    23161 tgtagtagcg acggtcaatc cgccaaaagt atctaaaatg attgccagaa taaccaatac
    23221 aaacattgct aatggataat cctttttagg gttttccatt tcatttgcat gactggcaga
    23281 tgtttctacg cccatatatg ctaaaataaa ggaaacaaaa acaaccagcg taggcaattt
    23341 cgaaaaatca ggaatcaatg ttttcataga cagtatcgtt tgtagtggag cgcctgaagc
    23401 gacataatgg atccctaagc caaacaagac taatgctggc aacaagatac cagctacaaa
    23461 accgatccga gcaatcaatg aagtgtattt ggttcctttt aactgtgaga gtaaaatagc
    23521 ccagaaaata atcaatagga ttgccaattt cagatattta ttgttttgta tagcaccaat
    23581 acccgttgca taggataatg caccagttaa gaaatacagc atggtcaagt agccaactgt
    23641 tatctcaaac cattggaaaa agattgcggc aaaaccgaat cgttttccca atgtgcgact
    23701 gacccaagta tagacaccgc cggtttccct tagttaaaaa tattactctt gttgattact
    23761 caactcttcc aataaatcgg taatatatct caatttcctg tattctttcg ttatcctcat
    23821 tcgttgttgc ttcatgtatg aggatttttt ctatcatggc atttaataat ggtgctgtca
    23881 gttcctttgg tacggaatat tccttgatta actcaatcca cttttcagca cctatcatat
    23941 cctgttccat tttacttagt tcttctctta ggcttgttat ctgctgttcc agttctatct
    24001 gttctttttg gtacttgctg gacaacatca caaaatttcg ctctgttatc ttctcacagg
    24061 ctctatcttc atacattttc gcaaataaac ggtcaatctc attttgtctg ttctcggctt
    24121 tcttcaatgt acttgccttt ttcttctttt cccgtattct ctctgcattg ccagctttct
    24181 gtatcttgtt caataccttt tcttcgtcct gctgtactgc cttagcccaa tactgcaagc
    24241 gttccaatac ggcttgatac agcacatcat aacgaatgta gtgcatagaa caattacctt
    24301 tgccaaactg cccgtagtaa ctgcaagcat aataactgta aggcgtttta tttgtcttat
    24361 tcgttccaaa tctcatagac caaccacaat ccgcacactt gacaagccct gcaaaaatcg
    24421 gcgttgcctt ttcctttgtc tgtctgcgtc ttgattttat ctgctcctgc acacgataga
    24481 acacttcctt gtcaataatc ggctcgtgag tgttttctac tcgaaaccat tcactttcgg
    24541 gcttacgcac tttctttttg ctcttgaacg ataccgtaga ctgcatattg tgaacactgt
    24601 ttccgatata aacctcactt ttcaatatcg ccttgacatg agcaatcgtc cactcataac
    24661 gcttactttc gggttttccc tcaaagatat gtgcaaaagt accgtatctt gtaaaattta
    24721 gccatgacgc tgtcggaact ttttctgctc ttaactgctt tgtgattttt gcacttccgt
    24781 aaccttgata ggctagggaa aagatttttt caataatcca ttttgtttca tcatcaacca
    24841 acagttttcc tttgatgtca gggtgtttct tatatcctaa cggagcataa gcaccgtaat
    24901 gagccccctc cgcaaacttt gtcttaaatg ctgatttgac tttacggctt gtatctcttg
    24961 ccacccattc attgatgatg ttcttaaatg gtgcaatctc actttcgcct ttttcgctgt
    25021 ctacaccgtc atcaatcgct atgtagcgga cattatgact agggaaatac aattctgtat
    25081 attgtcctgt cataatataa tttctgccaa gacgggaaag gtctttcgtt acaacacagt
    25141 tgatttttcc tgcctctata tcctcaatca ttctttgaaa actcggtctg tcaaaatttg
    25201 ttcccgacca gccgtcatca atatattcat caatgacatt caaatgatgt tcttttgcat
    25261 ataaacgcaa catacttctc tgtgtggtaa tgctggaact ttcgccctgt aattcatcat
    25321 ctcggcttaa cctaagataa agtgcagtat tgtaaatctg ttgtttcatt gtcagttatc
    25381 ctcctttata actaacccgt gtttacaata cctgcttgca agtaaagtat aacacgggta
    25441 ctttttgtca ttcaatctat ttcttacaac tgcaccgatt ttatgcgatt gtctgctttg
    25501 ctttttccag catgacatca gttagaagtt cttccattgt tttgcccttt tcggaaaaat
    25561 gctcttttac tacaatcttt gtctttcctc tgttgccaat gccacacttg ccatttttct
    25621 tttgaattgt ttttacagca ttatcaataa cacatacccc ctttctgtaa aatctttcta
    25681 agcaattcaa cggcttttgt catagcccac aggagttcca cctctgcata gttcttatgt
    25741 agccgtaccc attgcgtgcg acgctcttaa tgctcaagct gtggctgtac gggagtatca
    25801 ttatctgaca gtacaggtca tggcgataaa agaggacaaa tcttttacaa gaacacaaat
    25861 aggttttcgc tcgctcttgc aggggaaagg tcgtggcgta tttgtttcaa cggctagctg
    25921 tctgtcaaac gtattgaata gctttattca gttgtcaaag aacgattgaa agaaaaaaat
    25981 cgtctttcct atatatcgcg ttggaaaaga ggagaaacgt aaccaaaatc caaaactttt
    26041 tccattatta gtacaaatgg aacggttcat tttggcaatc aacggttgat tttctccttt
    26101 ttcactacta tctgtacagc aaacgtccat ttgctaagtt taaatggaaa aattttaact
    26161 tcttttcatg caccatatag ggatagaaaa tgtcattttg ctaagttgat gatgaaaaaa
    26221 gaatattttc cttttcacac cctaactacc caaaccatac cgttactgcc aacaatagaa
    26281 tgaaatattt tctttgcacc atatagggaa cggaaacctt aaattgttaa gttgagaaca
    26341 aagtattttc atcttttcac atcaacactc aactgctaat gcctgtttga atacaaaatg
    26401 atgatgtttt tcctcttttc acagaagact gtccactaat cacgaatttc cgtcattttt
    26461 tcaaaacttt tttcttcttt cacttcatat agggacagca agccccgatt taatgaccta
    26521 ttttcaaaaa aagtttagat ttccttttct cgcaccatat ctgtacgcaa gtagccattt
    26581 tgttgcagat ttaataaaaa atttattttt cttctttcac ttcacatagg gaatgaaaac
    26641 agctctttgc tgactaaatc tgaaattttt caattacatt aaaaaatacc gtcattctct
    26701 tgtggaacag cggtatctat acgttcttct atggttgatt tctctaattg taataaatgt
    26761 ttatgcttgt cttttagttc tgcctgctca atcatatctt tcagtatcgt tgtacgattt
    26821 tctctgtcta atgcctgtac catttttatg gttggtgcga cttgtctttg tagccaatgc
    26881 aacgcttttt gtaaagtgta aggctctggc tttgtggtta agcgtatcgg ctctctgttt
    26941 tcccctacaa accatgccca atcatcattt gttatccatt gactttttgg cttgtcgtcc
    27001 tctctgtcaa caaagcggac ataatgattg atgatagaaa aagcggtgcg ttcagcgtcc
    27061 cgataggtca gtaaatctac aaccgcataa taggctcgtt cgttcttcat gcgtatttca
    27121 aaacggttct taatctccgt atcttcaatt tctgtgccat tcttgacata ctgctcataa
    27181 tttttctgat aagcacacat atataattcg ctacttgttg aaccgagata taaggtttct
    27241 cctgtattct ttggtaaatc gctaccgcat ttttctgtac cgctgtaatc tttctgcata
    27301 cggaagtagg acatacattc gccagccttg tatttttcct ttaactttgg aatatccaat
    27361 attcctgctt tatcattgat agccaaatca agccgtttca tcacaccacc agccgtcata
    27421 caatccagca tgaagtcata ccatgagcgt tcctgtgcca gcagataaca ttccatttgc
    27481 cgacagcctt tgcctttcag ttccaataaa acacctaaat gctcctgcat ggaacacatg
    27541 atattgatgt cgccaagtac atatttgctt tcatatccgt attttccata atcttcataa
    27601 agcatgtaat tagatttcag ttgtaataca tcacggataa tcgccaatgc gtccgtagta
    27661 ggaaagcgga cacgaaagta atcaatcaat aagaatagtg gttcttgcgg attgaaacgc
    27721 tcaatctgtt tttctatgat ttctcttaat tcgtcattca atggctgttt tcctttttca
    27781 atgcggttgt aatattcacg gctgatacca caggcaacag caagccttgt ttgtgttact
    27841 ccgtattcct cacgcttttg tcttagttct tcaataaagt ttttatcatt cattcttcaa
    27901 ttcctccaat caaaaaaggc aactaccata attgataatt gcctgtcatt aaaaatctgt
    27961 atagaaaaag cagtcaatcc taagactaac tgctttgtgt gtatggatat aaaattgctt
    28021 ctaatcttga ataggtatcc aaatttcaat aactgaatta tcactattcc aatgaaaacg
    28081 ataatcgtat ttttcaaagt gtaggataat atcttttata ggaatatacg atgtactagg
    28141 aataatttct tcataaatct tgccataagt ttcatatatt ttatccatag caccaatatg
    28201 ctccacaacc aaatatttat aagacggtat ttctttatac agaaagccct ctggggtaat
    28261 ggttctttgg ggaatagaac agaaataata aagttttcca tttcttcttt ccataaaagc
    28321 atattttacc caatttcctg attgtgaaag atacgctttt ttcaaactac tgttaaactt
    28381 tctccaaaac tgtgtactaa tctgaatatt ctttttttga taattagtca gttctacttc
    28441 ctgcccaata actgcaaatg cgtttaattc ctttatagaa aaatttatca ttttaccttg
    28501 tgagcagtga taacggtata actgtacgca tttaccgtta taatacaatt atcaatcgtt
    28561 atataccagt tttttccttt tcttgtgatt tccgcattag aaaggctaat tttttctttg
    28621 caccattcaa ttacattttc tacattcaaa gataagttct ttttaattct aataacgcca
    28681 agttcagttg tgtgaagtct tcctaagttt tccagtaatt cattttccat attttgatac
    28741 ctcatttttc agcaaattta tttgtatgtt ttatacccca attataacat tctcatatca
    28801 gcactacaat aaaaataatt aagcaccaat aatcttattg aacatctcca accgtaaatc
    28861 tttcacacca tcagtgataa acactgagct gacagcaatt acgacaacta ccataattgc
    28921 ctgcacttaa atttctgcaa aagaaaacga caatcaattt cttatttttt gaaatcaact
    28981 gtctttctca tatttactat tcaattttca tagtgatata ggtacaattt atatcactac
    29041 tgtaaaaatg gggttttgcc ttataaatac agcgttttcc gcttgttagt gtatagtgtt
    29101 tcttgttttt tgtgcccccc ttgttagata acgggggctt tgaacggctc gcaagctcgc
    29161 cgaaccgccg aagcgaaccg cccccttgca agcatggcgg aaaaatctta aggattttcc
    29221 cgccaacgtg gtcggatcgc tccgtcggtt taacagcttt tctgtttctc tctagttatc
    29281 tacgcatata aatcaaatcg gttattccgt tataagtctg tgttttacaa agccttaact
    29341 ttatttccct gtctatgttg cccaaaagac gaaccccatt tcctaacaga gtaggaataa
    29401 cagaaatgta atatttatca atcatatttt tgcttaccaa ctgctgaaca agatttgctc
    29461 cgccacaaat ccaaatatcc tttcctgctt cttgttttag ccgttccaat aataaaacag
    29521 gattttcact tgtaaaatga atttgttggg aagaattgca ctctttatgt gttaatacat
    29581 aagttgtaaa tttattatat acccattctt gcggagaaag ttcagtaaca acctgatgag
    29641 aagtattcca ccccattaag acagtatcta tatctttggt aaattctgta taagtgtcga
    29701 tgttttcatt gtcatttcca tgaccattta gccaagctac tccaccatta ccgtccgcaa
    29761 tatatccgtc aaggctcatg gcaataaata aaactacttt tctcattttt tctcttttat
    29821 aaatacaaac aggaatttat ttctcttttt tactcataag ggaacaactg attgctacaa
    29881 ataaacccat taaaatccat ggaaatgctg ctcttataaa atcactaacc atactttatt
    29941 cctccttaaa ttccaatttc cttagttcta caaactgaaa tttttgccta tgaatgtatc
    30001 ttgttcttta tacgcaaaca ggcttctgtg tgcataatcc aatgtcttga aaacggcatt
    30061 tgaattagtc cacaaatatc tttattacac caataatcaa tcagccaaat tgctttttca
    30121 ttgtcgttta ctacattcaa cgattctaag tatccttttc tttctttcgg tatttttctt
    30181 ttcaattcat cataagataa ccgttcaagc attttctcag tgctttccca tacttcgaaa
    30241 atatataaat ataattcttc cagattaagc tgttttgaaa agtctgcaat ctgctgtttc
    30301 atgagttcat ttcctgttgt aatgataggt gaattgatac gctcttgata attgcctgca
    30361 aaaaatactt gttcatcttc atttatcact gtatgcacaa ctatatcttc aatgcggaaa
    30421 atatgccaaa tagaataagc aatcgtctta ctatgataac catcagcgtt tatgaagggg
    30481 attgagttaa aatcttctct gcataattct tctttgaaag atactaaggt ttgtattaac
    30541 tgacttcgta aagtaagtaa agtatcaata cccctcttat aagtatcttt cttttttatt
    30601 tgtgcctgca ttgttttatt aagttcagac cattccttat tcatattcaa tcctctctca
    30661 aattctgatt ttatcgcccc atacaaactg gaatttgtct tacaacaatt tatatatgca
    30721 taaaaagcaa tatatttatg aaaacaccct aaatccaaaa tttactcaat tcattttttc
    30781 cttacataaa atgttctcgt atatttcact tcctcaccaa gtgtttcccc tatcaaaact
    30841 tcaaaatcat tgatatcaaa ttctggaaag aatgtatctc catcttcaat atttaaatct
    30901 acttctgtga tatacatttt atctactatt tgcaaagctt ccttaaatag tccatatcca
    30961 ccagatatgt atacatctcg tcctttagcc aataataatg catcttctaa tgatttaact
    31021 gaaactaaat tatctccttg atactctgtt gtggtggaaa caacaatatt cattctatta
    31081 ggcaacggat gaccgatttc ttcataagac tttcgcccca taataaccac attacccgtt
    31141 gttaactctc taaattgctt ttgttctccc tttattttcc atggtatatt accattcttg
    31201 cctataacat tattctttga cctcgcaaca atcaaaccta tcatctattt catcctccat
    31261 aatatgatgt cgtacctgtg attccatcaa gtgcaccaca tcttaacaaa ctcaaaatta
    31321 tctcgaacta accagctcga caaattcctt ttttgtcttc atctgcatta tatcatattg
    31381 ttataagcat tgccatttct tttctgcttt tgaaaagtta cttatcagcc tatgttttgt
    31441 aaagggtgat attcagcatt tctcacgcaa caagcccttg acaaaccata gactaataag
    31501 ttttaaggta atcaagcaga aatgaaatag tctgctgttt cacggcaaca aaaaagagat
    31561 accaacacac ttatgtagta tctcttagtc tagtagcaat attcttttgt ttgcaagcag
    31621 gtaaacatgg gctgtttctt aatatctttt tgtttgggaa acaccttttt cccatccttt
    31681 caccgttgcc atctctgctg cgcacaatgc cacagggata aaccatagca aaccgcctaa
    31741 taacaagaaa aatactaagc tgaagcctga ggtcgcgaat gtgggatatt catataatga
    31801 aacgaccatt gagatggtca tagccaaaaa gccaaagaac gttaattttt ttgcaggaat
    31861 tttcgttttg ctttgttgca tgaatcttcc tcttttctat ttgaatttgt ttcaattgag
    31921 tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt tcttcttttg tagtagtgat
    31981 cgttgtaaag tgcatcgccg attttaaaat cactgttgtc agcacatagg catacacggc
    32041 agtaatgatg acaccaatca cttgaatccc taagaagtac cagctgcttg gattcgttag
    32101 taaatggctg ttagatgcaa agatccctat cagaatcgta cctgtgaatc cgcccatgcc
    32161 atgaacaccc cacacatcaa gtgcatcatc ccattgtttc tttttacgaa aatcaacagc
    32221 agcatgacag acgatacccg caatgatccc aatcagcatg gcactttttg cttctacata
    32281 accggcacaa ggcgtaatgg ctgccaatcc agcaacggat cctgttagta cgtcgacaaa
    32341 atcaaagcca tttcctttta cttttgcaat gatcagccaa gtaatcatcg caaatgcaag
    32401 agcgatgaat gttgaaccaa atgcattgac tgcttgttca tctgctcgaa gtgcaccacc
    32461 ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta ccgatagcag ctgccataag
    32521 actgctatgc tgacttcttt ctcctaaagg aatctttctt tgaccaagtg tgaggataca
    32581 tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct ccaccagcga aatctctaaa
    32641 tcccatctga tccaaaaatc cgccacccca tatccagtgg caaacaggga aataaatcag
    32701 cagattccaa aaaataacaa gtataatata gccttttaaa ttcaatctcc ctgcaaaagc
    32761 tcctgtcatc aaaggaacgg taatcacgat aaacatcagt tggtaaagaa agaacattaa
    32821 aaatggaatc gttgcaccat gaaacatgtt ggggaagctt gtaacatgtc tcatcaaaaa
    32881 gaaatctgcc gggtttccaa taacaccgcc gatatcccgt ccaaaggcca atccgaatcc
    32941 accaaatacc cacataatcg ttgttatccc aacggagaga aaagattgag acatgatcgt
    33001 tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa gctagaccag gagtcatcag
    33061 gcaaaccatt gctgtacata aaatcatgaa tgctgtatct cctgtattga taggtaatgt
    33121 attcattttt ttcgcctcct ttctacgatt acgagtataa aagaaggtgt agttgttttt
    33181 ttcttctgtt tttttgaatt aatacttgca tttataaaag acagacaatt aataaaagct
    33241 tgatgtgttg tgctcttttt cgtttttcac acaaataaag acaaaaaata aacggtcttc
    33301 tttttataga aaaccgttta ttttcgtctt tttttatttt taaagatatt ttttcataag
    33361 gaataaagaa tacttcatat aaaatatata aaaaagaacc gcagcgagtg tcgctacggt
    33421 tctcaaattt gttcttcatt tcaacataag tttttcttca atttgcttat aatttacgtg
    33481 caaagaatga aacgactaat actaaaatga ttgcaccgat caatgatgga atgatagcca
    33541 ttcctgcaat tgttggtccc caagttccta gtaaagcttg tccaattgct gaacctacta
    33601 aaccagcaat gatgtttgcg atgatcccca ttgatgatcc tttattcgtg attgctcctg
    33661 caattgctcc aatgataccg ccgacgatta atgcccaaat aaatcccatt ttaaattcct
    33721 cctaactgac ttcttacttc ttattgtaca cgtggttcgt tattttcttt gactttttgt
    33781 gcacctttgc ctacagcaga ttttgcttta tctgtttggc gtgaagcaaa ttgtcctgtg
    33841 gattttgctg cttctgttac tttgtcttgt actgtttctt gatctttttg gtattcttct
    33901 tgtgttttga tgtctgcaac attcacattc acttcgataa catctaaatg tgtcatattg
    33961 tttacttcag tactgatcaa ttctttgatt tggttgtaaa tatcttcgat gtctttaccg
    34021 tattctacga tcacatctag atccacagct acttgtttct tacctacttc tgtattgata
    34081 cctgctgtta cgttatctgt gttgactaat ttttcagcca tatttgagaa aaatccgcca
    34141 tctaatgtca atagaccatc tacatctttc aatgcataac cgatgatttt ttggatgact
    34201 ttgtcttcat aagtcaattc accattcatc atgcggtttt ctcctttttc atttgccaaa
    34261 actgatccat ttgttgtatt catgtttttg ttttccatct ttgtttcctc ctatgattta
    34321 aaaaattgat caactattcc tgactgtttc aaataaaaac cgattcctgc gccaattgct
    34381 gtcatgatta tgatcaacaa tgtcttgaaa aatccaatcg ttaaccataa aacagctagc
    34441 agtagtccaa gacccccaaa gataataggg agcttgtatg ctgaaaatag ttctttcatt
    34501 atttggccct cctattccac tcgagagtgc ttgtagtctc ttgtatcttc ttgttcttca
    34561 taaccgctgt atgtgactgc cactttgacc gtttcttttg ttccgagtac tttagttact
    34621 tcttcttcga tatcttgcat caatacacct gttttcccta ctaatgaaga agttcgtttc
    34681 aagtctccgt tgacatggat atggattcga tttttcgttg aacgtatccg tactttaggt
    34741 gaatgaatga actcttcttc gtgtaaatgg gaacgtacca acccttcgat cgcttttttg
    34801 tcgattgtta gtttgccatc ttcccgtttc ataacgaaag ttcctcttga ctttggatag
    34861 aaaagaacga ctagcaagat gataatagca agaacggcaa atactacagc tgcccagaaa
    34921 atatattgtt gcatgtagta attcgttaaa gtaaagaagc gaaatcgttc caattgttca
    34981 ggcataatat acggcgcatt gatcaacgca atcgctagaa atactgaaag tagcagtaaa
    35041 ctcatgataa caccgattat ttttaacccc ttattcattt tccacctcct ataaacttat
    35101 ttttaccctg taaataactc taccgacaat cacagtatag atagcgaaca gttagcatag
    35161 catcagtaaa taaagcgcta ccaaacattg ctggatcata acggcgtttt attgccagtc
    35221 tgatactgaa ataacaattg cttacttatt ctctatttac ttttgattgc tggctatact
    35281 taaaggatat tattcaagat taaaaattgc aaagattatg ctcagttatt attacaagat
    35341 tctatttatt tattaaattt tttcggagaa ttaaaaaata caataaataa ctttagttac
    35401 tttaatgcgt tcttacttta tttttatatt atcatttttc ttattcactt ctttggttta
    35461 tactattctt ataaataaaa acgaggtatg tcctatgatg aaaagattgt actattcatt
    35521 gatcataacg attggctatt tgattgtatc aaatctagga aatatggtat ttggtatcag
    35581 taaagaattc agttggacta ctacattatg ggaatctcta ttctttttca tcttcgtatt
    35641 tttgttacaa aattatcgaa aaaaataaac atgtgccatc tatctgttgg cacatgtttt
    35701 aggatttatg gtacttttta ttcggcaatc cattttattt ttctacttct tcttaatacc
    35761 tctaacactt ttgatttata aactaatcca atgcatctac ttgcgcttat taagctattt
    35821 cagctagata gtaccacccc atgcgtcaat gcttgatttt ccttttgtat aacattgtat
    35881 aagcaataaa ggacaaataa gtggtgatta caccaaacca ttgaataaga aaaattctga
    35941 tacgtaaata aacatgaact tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc
    36001 ttttttctca tacagttctg ttaacttaca cttctcttcg ctagataata aaaaatcctc
    36061 caactgtatt gtccaatata atcccaggca tctaaactag cattatagaa gagacacaca
    36121 tagaatctaa aataactggt tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca
    36181 ctccacacct tcatactcta tcatttttta taaacgatat atttttattg tttttcgata
    36241 aaaaatagtt gttttacaat aattattctg ctatatttta gctatcaaag ataaaagagg
    36301 gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta taagtgccgt
    36361 atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa aaggattcaa
    36421 ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc tcctagggat
    36481 cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc aagctttttc
    36541 cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta ttggtttcgc
    36601 cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg ctccaggttt
    36661 gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat taacaagcgt
    36721 gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt tgacagtgtg
    36781 aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa aaaaatgagt
    36841 gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat tctaaaaaat
    36901 ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt tttggagtgt
    36961 caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga agaataaaat
    37021 aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga gacttttaga
    37081 agttatttac gtctattatt cagatttttt atttcgattg aataccgttt tatctataaa
    37141 taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact atcacttttc
    37201 ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat aatactttat
    37261 tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt attacaggcc
    37321 attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg tgttaactct
    37381 tactccattc ttttttagta agctcccata tatactgtaa agccttctat atattatgtc
    37441 atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata acagtgatgg
    37501 acaattcttt tttattttta acattatttt gataataatg
//
LOCUS       DACCCR010000021        36853 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCCR010000021 DACCCR010000000
VERSION     DACCCR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.021"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(324..893)
                     /locus_tag="IVK93_001062"
     CDS             complement(324..893)
                     /locus_tag="IVK93_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6386367.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="IVK93_001063"
     CDS             complement(967..2256)
                     /locus_tag="IVK93_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386368.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="IVK93_001064"
     CDS             2610..3458
                     /locus_tag="IVK93_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP6386369.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="IVK93_001065"
     CDS             3472..4545
                     /locus_tag="IVK93_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP6386370.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="IVK93_001066"
     CDS             4535..6706
                     /locus_tag="IVK93_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAP6386371.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="IVK93_001067"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="IVK93_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP6386372.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="IVK93_001068"
     CDS             complement(9009..11195)
                     /locus_tag="IVK93_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP6386373.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="IVK93_001069"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="IVK93_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP6386374.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="IVK93_001070"
     CDS             complement(11417..11857)
                     /locus_tag="IVK93_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP6386375.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12404)
                     /locus_tag="IVK93_001071"
     CDS             complement(11931..12404)
                     /locus_tag="IVK93_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAP6386376.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12714..14180)
                     /locus_tag="IVK93_001072"
     CDS             complement(12714..14180)
                     /locus_tag="IVK93_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6386377.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="IVK93_001073"
     CDS             complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="IVK93_001073"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6386378.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(16717..16905)
                     /locus_tag="IVK93_001074"
                     /pseudo
     CDS             complement(16717..16905)
                     /locus_tag="IVK93_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(17099..17458)
                     /locus_tag="IVK93_001075"
     CDS             complement(17099..17458)
                     /locus_tag="IVK93_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP6386379.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17488..17829)
                     /locus_tag="IVK93_001076"
     CDS             complement(17488..17829)
                     /locus_tag="IVK93_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386380.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17826..18500)
                     /locus_tag="IVK93_001077"
     CDS             complement(17826..18500)
                     /locus_tag="IVK93_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6386381.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19295..20593)
                     /gene="asnS"
                     /locus_tag="IVK93_001078"
     CDS             complement(19295..20593)
                     /gene="asnS"
                     /locus_tag="IVK93_001078"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP6386382.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20633..21823)
                     /locus_tag="IVK93_001079"
     CDS             complement(20633..21823)
                     /locus_tag="IVK93_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6386383.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(21844..22371)
                     /locus_tag="IVK93_001080"
     CDS             complement(21844..22371)
                     /locus_tag="IVK93_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6386384.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22418..25207)
                     /locus_tag="IVK93_001081"
     CDS             complement(22418..25207)
                     /locus_tag="IVK93_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6386385.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKTAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(25354..25554)
                     /locus_tag="IVK93_001082"
     CDS             complement(25354..25554)
                     /locus_tag="IVK93_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6386386.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(26006..26326)
                     /locus_tag="IVK93_001083"
     CDS             complement(26006..26326)
                     /locus_tag="IVK93_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386387.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(26627..27745)
                     /locus_tag="IVK93_001084"
     CDS             complement(26627..27745)
                     /locus_tag="IVK93_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386388.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            28216..29523
                     /locus_tag="IVK93_001085"
     CDS             28216..29523
                     /locus_tag="IVK93_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP6386389.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            29642..30841
                     /locus_tag="IVK93_001086"
     CDS             29642..30841
                     /locus_tag="IVK93_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6386390.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(30868..32136)
                     /locus_tag="IVK93_001087"
     CDS             complement(30868..32136)
                     /locus_tag="IVK93_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP6386391.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(32299..32946)
                     /locus_tag="IVK93_001088"
     CDS             complement(32299..32946)
                     /locus_tag="IVK93_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP6386392.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(33354..33857)
                     /locus_tag="IVK93_001089"
     CDS             complement(33354..33857)
                     /locus_tag="IVK93_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6386393.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(33980..34744)
                     /locus_tag="IVK93_001090"
     CDS             complement(33980..34744)
                     /locus_tag="IVK93_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP6386394.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            34851..35126
                     /locus_tag="IVK93_001091"
     CDS             34851..35126
                     /locus_tag="IVK93_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP6386395.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            35842..36792
                     /gene="yidC"
                     /locus_tag="IVK93_001092"
     CDS             35842..36792
                     /gene="yidC"
                     /locus_tag="IVK93_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6386396.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT        10593 a         7757 c         6534 g        11969 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataaa
    12541 tggtgttcaa tcagctgacc ttcagcatta gctcaaaaaa cggcaaaata tgaggagcta
    12601 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    12661 cggtgttcca gaagtagctt ctgtcacaac ctcttgtaac aagtatttct attttaaggc
    12721 tgaaccactg tttctggttc agtaatttct ttgtttttgt ctcgacgagc aattgctggc
    12781 aagatcatac ctaataagat caagacaatt ggtgtgatga tatttgtgaa taattggaac
    12841 caccatgctt taggatcagc agtataatct acttttggca ccattcctag gatacatgca
    12901 aacgcagtga aggcaaagca ccaagcacca aagataaaac cgatttttgg atttttaaca
    12961 aatttatatt cagaattgaa tttcttataa gctttattca acatcatata tgccaagaac
    13021 acccataggt aacgcattgg catgacaact gagttcaagt tcgtcaacca ttttactaat
    13081 tcattcatat ctttgatacc aaagatcggc aacaaaataa tgattcctac tagtatacct
    13141 gtcagaagat aaccattcac caatgtacct ttacgattac gtttacgtag ccagtttggt
    13201 acaaattctg gatccgcatc tgctaacaag atttttagtg gtgcatcaat tgaaaaggct
    13261 aatgcggaga tttgtccaat catgtttgtc aatgcgtaaa tgatcatcaa tgagttaccg
    13321 ataccatagt aattacctaa aatttgaaaa gcactgtatg ccccatttgc catcaagtct
    13381 ttagggatat tatcgctagc aaacagcata cccattgcga ctgagcctaa tacagcacaa
    13441 atccctacca ttgctgctaa gaagatcatc cctttaggaa attcctttgc aggatttctt
    13501 gtagagttga cataagggga tattttttcc gcccctccaa ctgcaaatac tagcatcgaa
    13561 acggttgtga aataactgaa atcaaatgtt ggaatatatg ttttcacttg attcatatgt
    13621 ggtgtggcaa attccatcgt tggattgatg aacggtgccg ttacagctaa aagaataaat
    13681 aagatcgaca tgacgaacat cgctgttccg gccagtccgc caatcacttt caaagtagaa
    13741 agtccttttg ttgataaata taaaaaggca agaaatataa taagtgaaat aactgctacc
    13801 atttgtactg acatcgtatt tacgatattt ccatttcctt ggactgccca accaccagca
    13861 atcagaattg cttgaggctt ttgtgccaaa taaggaatat ggacgaccca gtaagtccaa
    13921 gcggcatagt atgccagtcg tttattagat gtcgtatttt cgacccatga actgacgcct
    13981 cctttactat ccttgaaggc tgatcctaat tgtccaacga tcaatgcgta tggaataaaa
    14041 taaatggcca aaatcaatag ccaagaggtg acaactgata ctccttgctg agcatagtta
    14101 ttaactacat tccccatgcc ccaaaccatg ttaaatgcaa ttaacgcaac agtaaaccag
    14161 cgtaattgtt tgttgttcat tcgttctctc ctttatgata aaaatttgtt atccagatgt
    14221 aatcatattc aatttttaac aaacttacaa gacagtgtta ttttatgtgt gtattctttt
    14281 atttaataca catcgttttt tcatattttt tccatcaaaa aaataagtta ttttctttta
    14341 tttcattgac cttattgaat ttcaagctat tttgaatttt ctgcaaaatt aaaaacatta
    14401 gttattttat aatcctatct attttcttta catttttcat taagaaaagg atataaaaaa
    14461 aagaggacgt tcattcgtcc tcttcattga tcaacgttta ttttttacat gcaagcttcg
    14521 catggcattc aaaacagcta atactgtcac cccaacatct gcaaatactg catctcccat
    14581 tgatgcaaaa ccaaaagcac ctaaggaaag taccaaaatt tttatcccaa tagcaaagac
    14641 aatgttctgt ttgacgattt tcaatgtttt ctttgctaaa tggattgctt ctgcaatctt
    14701 cgatggttga tcattcatga tgactacatc tgctgcttca attgcagcat cgcttcccaa
    14761 tccacccatt gcgattccta catctgctct tgctagcacc ggtgcatcgt tgattccatc
    14821 tccgacaaat gcggtctttt tattgttact ttgtaagatt tcctctaacc gctgcacttt
    14881 atcttctgga agcaattcgc tatatatctt gtccacgcct acttgcttcc cgatatgatc
    14941 agcaattttt ttcgagtcgc ccgtcaacat gactgtgttt ttcacaccag cctgttttaa
    15001 ttgaaccaat gcgtctacgg catcttcttt caaggtatct gcaataacaa gataaccact
    15061 gtaactttga tcgatcgcta gataaagcaa tgtaccgatt tcttgacttg aaggaaaaga
    15121 gatttcaaat ctttccatca atttgtgatt acctactaat acttgtttgc catcaacttc
    15181 agctaagatc ccttcacctg caaattcctg gatattagct gttggtaaaa gcggacctgc
    15241 gtagccttct aaaacagatt gagcaatcgg atgtgtagaa aactgttctg cacttgcaac
    15301 atattgcata aatttctttg gatcaattcc tgttggatcg atcgtctgga tcgaaaagtc
    15361 acctttcgtt aatgtgccag ttttatcgaa taccactgtc tcagtttgtg caagcaactc
    15421 caagtaattt gaacctttta ctaatacacc gattttactt gctcctccga taccgccaaa
    15481 gaaactaagt ggcacggaaa tcactaatgc acatggacat gagatcacta aaaatgtcaa
    15541 tgctcgatag atccattcag aaaacgccgc tctagaaaca acaagtggag gtaaaacagc
    15601 taataaaacg gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa
    15661 attttcagct ggtgcttttt tactgctggc attttctact aattctaata atttgctgat
    15721 agtagaattt tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa
    15781 tccgcttaat actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc
    15841 tgacgtatcg actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc
    15901 aggcttcaca agtacaaact gaccgatcag cacgtcttca gggttcacgg tcatcaatcc
    15961 ttccgccgtc tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt
    16021 tcttgaatga ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac
    16081 tgctacggct tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag
    16141 aaaattctca tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc
    16201 atatcctatc agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc
    16261 aaataatagc aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct
    16321 ctttttttca tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc
    16381 tggttctaat tccttcacta ctttttttgt ttcttgttca atcgtagcca aatgatcttc
    16441 ctctgcgtca atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt
    16501 gattttctga actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa
    16561 tctgtaactt ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact
    16621 aattataaat gaatggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc
    16681 tgcatcggaa aatttcttcc atacagatgt tttggcttat tctttctttt tctcttcgat
    16741 cattgccact gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact
    16801 aagtttgaag cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata
    16861 tcttttttca atccctgcaa aatgtccttc ccagatatac gaagctccac tacgctttca
    16921 aaaatataga aagagttctt taatgaaaat tatttcaact ttccataaac agtaacccca
    16981 tataaataaa agacgtaaaa gtcacataga agaacttata caggaaaacg catagagtca
    17041 ggtgaaatac atgtatccca cctggctcta tgctttatct aacctttatt attctctttt
    17101 atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg
    17161 caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag
    17221 caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac atgccagact
    17281 ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt
    17341 gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc
    17401 taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg
    17461 tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt ttattcttct
    17521 ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt ccgtattttc
    17581 ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca atcgttcttc
    17641 tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac
    17701 gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc
    17761 tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg cccaccttat
    17821 ttccatcatt tttcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg
    17881 acaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc
    17941 ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt gaatatacca
    18001 tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta
    18061 gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc
    18121 atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt tcgataaacg
    18181 gattcaaatt gctgagcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc
    18241 caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct
    18301 tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat
    18361 tcaaattcca tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct
    18421 ttacctagat cagttgcttc tgctcggtat cttcctgcgt ctgtaatgct atcaccgata
    18481 aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt
    18541 ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga
    18601 caccagccta gttccttcaa acaattgaat gaacgctcat caagtgtcta gttttttatg
    18661 ctattttttt cttaacgtgc cggattcccc ttgattcctt cgttgtttgg tccagagaga
    18721 tcgatcacac ccaagcttcc gtcaatcaga ccactgatga cgccatcgaa acatatattt
    18781 ataggctcat ctaagacttc tagtaaatgt ccctttacta atatctcttc tagattgctt
    18841 gggatagatt ggtcataact ttatctggtc cgatgatttc aagggtgaat tgaacttaca
    18901 tatggattat tgcatgctga aaatacggac ctactccatc ttcgaatgtt actattagtt
    18961 tgttttcagg tgctagatgc tgctttattt caacagcaga agcatcatca aatcagattt
    19021 ccatttgaat taccttcttt gtaaatgttt gattgattgc gatttcgtta tagaaccttg
    19081 tgaatcaaat agcaatttct tttgctttct tctcttgtct gctggaaact tccattatcc
    19141 cgtatttaaa aaaaagaact gcgagagttc gcagttcttt tttttaaata aacgttgctc
    19201 tccggccaca agtcacaata gtcatcaaac atgaaaaact gttttttgaa attactcctt
    19261 gtgactcgga gctgaacggc aggcgcacaa cctcttatgg ataaattcgg ttcaacaaac
    19321 gtgggaatgg aatagcttca cgtacatggt cgataccagc aatccaagtg actgttcgtt
    19381 caagacctag accaaaacca gagtgcggaa cagaaccgta tttacgtaga tcaagatacc
    19441 atgaatattc tttttcatcc aaaccgtgat tacggatttg ttctagcaag aaatcatagt
    19501 ccgttgcccg ttcgcttccg ccaataattt caccgtaacc ttctggtgcg atcatatctg
    19561 cacaaattac cacgtcttct cttgttggat gtggtttcat gtagaatggc ttgatggctt
    19621 ttggatagtt taggataaag acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag
    19681 gtgaaccaaa gtcttcgccc cattcgatgt catcaaatcc atttttcttc agtaattcga
    19741 ttgcatcatc gtaagtaata cgagggaatg gcaattttgt ataagttttt aagacttctt
    19801 tgtctcgtcc tagtacatct aatgcgtatt cgcaatgatc taaaacattt tgtactaaga
    19861 atgctacata ttgttcctgt acttctaaac tttcttcttg atgagtgaaa gccatttctg
    19921 gttcgatcat ccagaattcg atcaaatgac gtcgtgtttt tgatttttca gcacggaaag
    19981 tcggaccgaa tgaaaagact tttccgaaag ccatagcagc agcttccatg tataattggc
    20041 cactttgaga taaataagct ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc
    20101 cttctggtgc agaaccagtc aagatcggcg gatcaatttt gacaaaatga tgttgattaa
    20161 agaattcata cgttgcacga atgatttcat ttctgatctg cataatcgca tgttgtcttg
    20221 aagaacgcag ccataaatga cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg
    20281 tgatcggata atcgtggctc tcgcttacca cttccacatt tgtaattccg atttcataac
    20341 caaattttga acgactgtct tcacggattt ctccagttac taacacggat gtttcttggt
    20401 gtagattttt agctgtttgg aaaacttctt ccgacacttc gctttttaca actacacctt
    20461 ggaaaaaggc tgttccgtct cttaattgta aaaatgtgat ttttccgctt gatcgcttat
    20521 ttgcgaccca agcgccgatt gttactgttt ctcccacatg gtttttcgaa tcgatgattt
    20581 gaatttgttc cacgtatgac tctcctttta tattccttga atttcttcag gattaatttt
    20641 gacttttact ctcaataaat tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat
    20701 cagtagcata actcatgcgg acattttctg gtgccccaaa gccttcgcca gtcacaagcg
    20761 ctacccctgt ttcttctaaa agatcttcta cccattgtgt gacattttca tatttacaca
    20821 tgcgtagcgt ttcttttaca ttaggaaaaa gatagaaagc tccttgcggc ttttcaagag
    20881 aaacacctgg cagttctgcc acaagcggat ataacgtatt caggcgttct tcaaaggcgt
    20941 tccgcatttc ttctactgta tcttgctcac ctttcaatgc ttcaacagct gcatattgac
    21001 tgactgctgt cggattactg gttgactggg aagcaacagc aatcattcca ttgatgatct
    21061 cagaattccc caccgcataa ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc
    21121 cattgatgat gatggtctga tttttgattg cttctgaaat cgttgcaatt ggagtaaact
    21181 cattgccatt ataaactaaa cgaccataaa tatcatcagc aactatcaaa atatcatttt
    21241 ttactgccca ttcaccgatt gcctgaagtt cattcttgct ataaatcatt ccagtcggat
    21301 tcgatggaga attgatgatt acggcttttg ttttttctga acgtgcttgt tctagctgtt
    21361 caacagtaac tttccagcta ttggattctt cccctttcac aaagacagga agtccttctg
    21421 ccagttttac ttgctcaccg taactgaccc agtaaggcac aggaatgatg acttcatctt
    21481 gcgggtcaag gatcgtttgg aaaagggtat acaaagcaaa tttcgctccg tctgttacga
    21541 ctgtttgtga aggttcatag acaagtccat agtttttctt cagataatca acgattgcct
    21601 gtcgaagctc tgggattcct gctgttggtg tataatagct agctttccca ttgcggattg
    21661 cttcgattgc tgcttcttgg atgttttctg gtgtcgcaaa atccggttct cccacagtca
    21721 aactcagcac atctctgcct tttgctttta atgccttggc ctttgccgca gctgccaaag
    21781 tgacagacgg ttctaatcgt tttgttcttt ttgataactc catcccattc attcctttac
    21841 cttttaaatt cccttgattg tcttgacttc atctccagat tcaaagccaa tcaggtaata
    21901 attcagtttc ccatttgttt cttttcctac tacttcccac acaggctgat catcatacat
    21961 ccctaaagca gctttttcga tttggatttc aggatgggcc gcagcaattg tctgttttgc
    22021 ttcgttttca gtcagtccct ttgtttgatc caatactttt actttttcac cagatttagg
    22081 aatgataacg gcgatttctt ttcctttatc atttgtacct gtcagtgaaa agtatgtctt
    22141 ctttcgagta aaccaataaa aatgatcggt ttcctcaaga tttgcatatt tctttgcgat
    22201 ttctactgct tctttcttcg cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga
    22261 gctgatcacg atgaccgcta ataaaaatac gatcaagcca agtaacacgc gattaatcgt
    22321 tttttccttt gtctgttttt tcttcaaggg tcgtcgctcc tttatcttca ttccttttca
    22381 ttatagcaga ttttaattat gaaaaaagac agctcctcta acttccttct tcatttttct
    22441 ttaagaattc atggatatcc tgatagattt cctccatagc tagctctttg atcggtactt
    22501 ttttcggtaa tgcatggatg atccgtgcgc catattttgc agtgactagt cttcgatcca
    22561 aaataagcaa tacgccacga tctgtctccg tccttatcaa gcgacctaat gcttggcgta
    22621 acttcaaagc tgctttaggt acggattcct ggtaaaaagg attgatcccc tctgattcta
    22681 aataagcgtt cctagctttg accatcgggc gaagcggatt atcgaagggt aaacgtgtga
    22741 ctacaacgat ctgcaaagca tctccaggca aatctacacc ttcccaaaag ctgtcagcgc
    22801 caaacaatac gcttttttct gacaaaatga agcgtttcaa aagcttctcc cggcttccac
    22861 caaacccttg agctagtatt tctcgtcctt tttctagcat agacagatgc attttctgat
    22921 agaccttctg caaaacatca tgagaagtga ataacacaag tattggccga tcttcttgtt
    22981 cgatcagacc tgtaagtgct gtctctaaat aatcgatgta tgcttgattg ctgacttgat
    23041 ggacggcagg tgcctctttt ggcacataga tccttgcctg tttctcataa tcataaggag
    23101 aggcaatcac tttaagaggt gctgcttcaa tgccaagcct cttagagaga tagtgtcgat
    23161 tagggccgat cttcagcgtt cctccaatat aaagaatacg atcatatcga ggataccaaa
    23221 cagtcgacgg caacatagcc gcttggaaat catggagatg aaaaatcatc tgctgcgctg
    23281 tgttcccagg aacgaaccaa tggatcaaat gatctcgcca gtcttccagc cattgatcca
    23341 tcagcaagta ctgcttgtct aattgatcaa ataagctata taaacttgct aaatcgatcc
    23401 gcatctggtt tgtccagctt tcttggataa tctcaaaagt cagacgcatt tgtgactgta
    23461 gctgctgaat ctcctgataa aaaagctgca ggtgatcaat ggctgtccca gattccagtg
    23521 gcagctgatc cagcatctct tttgtcatca tgacttcttc tggatactgt ccgttgggtt
    23581 gcttgccgct cactaaactc attaatcctt ctgccataca aatctgtgct tcgcatagcg
    23641 cagaaagttc ttctcgataa atcgataacc agcgtaaggc ttcatgttct ttcggtaaat
    23701 agctttgtat tcggtcaaac agctgatttg gttcttctaa ttgctggatt tgtttatgga
    23761 aatagttaat acttaatcgt ttgtcattta ctttttcagc gatttccggc aaatgatgta
    23821 cttcatcgat caacaaaaat ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt
    23881 gtactaaaaa cgcatgattg acaatcaaaa aattactttg cctgattttc ttttgcaaga
    23941 aacgaacaaa gtcttgttcg taaaacgact gatttcttgc taaaaaccca gtacctctgt
    24001 gcgcgatatc tgcaaagaat gcatggttca agtttgttaa atgtaattca tccaaatcac
    24061 cggtcttcgt ttgcgtcagc caaactaaga ccgccatttg atacaaagca tattgttttt
    24121 gttctggtgg ttccacaagt gttcctttaa atcgttgcag gtcgatatag tggcgataac
    24181 ttttgataat tacaggttgt atgggctgat ctagcaattg attcagcaat ggaatttctt
    24241 tttctatgat ctgctgctgc aaaactagcg aaacggtact gatgacagcg gttttttcag
    24301 gtgtagccaa ataactcaat ggcaacagat aaccgattgt tttacccatt cctgtagctg
    24361 cttcgatcat caaatctttt gattcatctt ttgtgaaatg gtcgtagatc gcattcatca
    24421 gtctgttctg ttcttttcga tacatcagtg ttttaccaaa catcttttct tttgcttgtt
    24481 tttttcttgg atatgtccgc tcgccgtaat ggacggatgt gaatagttcc acttcttttt
    24541 tctgcaaagc tagcccatcg acgatttcta atgaggaatc aagtggctct ggtttttctt
    24601 tcatttcttc aatcacatga taaatgaaac gactcgtatc catacctgtg tgtctactta
    24661 atcgcgcaat ctgctctaac gtgatgattg gcaattttct catccgttct tcgatcagca
    24721 aaagaagttg acctgtcact tcggcatcac tatctgcctg atgaggattc tcatgaataa
    24781 atccaaggct ttctgacaaa tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt
    24841 gtgcaagttc tactgtatcg atgcctggta tcttcaaact aggaagtccg catcgcatga
    24901 gttcatggtt caaaaaatta taatcaaaat aaatattatg cgcgacaaat atggtgtctg
    24961 ccaaaagatt gtaaatcgtt tgggcgacat cttcgaaata aggcgctttt tggacgcgtt
    25021 gattcgtgat ccctgttaaa tgctggattt gttttgaaat cttccgccct ggatgaatat
    25081 cgatcgaaaa acgtgaagtg atccgacctg attcaatcat gacgcagcca aattggatga
    25141 tccgatcttc ttttggattc gtcccagtcg tttcgatatc aacgactgca taagttcttg
    25201 gtttcaaaat atcttcctct tctcctgcct ctaatgttct tttgaaatta tactacaaag
    25261 tgcagaaaaa tactctcatc gctttttaaa gaaaatagac aaaaacgccc agacacgcaa
    25321 atgtctgggc gtttattttt tagtataggt taattaagct ttagtaacgt ttgttgcttg
    25381 aggtccgcgt tggccttctt caacatcgaa agttactgct tggccttctt ctaaagtttt
    25441 gaatccgtcg ccttggatag ctgagaaatg aacgaataca tcgtttccgt tttctgcagt
    25501 gatgaatcca aatccttttt ctgagttaaa ccattttact gtacctgttt ccataataaa
    25561 aatcctcctt gtgtatcaac acatattttg caattacttg aatggtgtat atctggaaga
    25621 tgtttatttg aaacaagcta ccacagatta ccgaacaaaa actactccta tagtatatca
    25681 taccgtttac aaaaaagcta gagtttttcg acttttttta ttaaaaaaag ttttctcagc
    25741 gattacataa agtgaaaaaa cgaacttttt ttaaaataaa cgacatattg ttcagttggt
    25801 gtttttttgc tctgatgttc ttctttaaac taaaaaaatt attctttctg actacttatc
    25861 ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg
    25921 tatttctgat agcctatgac agtgaccaga agaacaaggc aaaccaatac gatcaacgag
    25981 attagttttt cttttttgct catatctata cctcataaat aacaggatga tgtccatcat
    26041 atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac
    26101 tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt
    26161 cttctgtttc aggaatcgca gaatcaagat ctgttgtctg tacataaaca tttttgtaca
    26221 gacgtgtgcc tgtaatagga gaccgctgtg tttcttcagc aaaaactggc tgacttgtta
    26281 aacttaggga tagaaacagc aaacaccata acagtctttt tttcataaag acctccttat
    26341 cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc
    26401 ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc
    26461 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
    26521 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
    26581 cctcttcctt aatactcagg tcaaaacact gatttcacaa cctcttttaa taacgaagaa
    26641 tcgctgtcag actcttttca tctggcgcat ctttttgatc caaaataaac actttcccgc
    26701 cattaatgat cacatcatct gctaatgcat tcaacacttg acgccgatca tattctgttt
    26761 caggatcttc accgaaacca tcagctaagt ttgacgtagc gataaataaa tgtgacactt
    26821 ttccttcttt cgcagctgga acgatatcaa ctaactgatc gatgaatttc ttatctaaga
    26881 gtttattgta ttcttttgat tcttgagctg cgagttcact tgacagcttc ttcgcccctt
    26941 cttcgatatc tctaaaggat agctgcgcag gagaagcaga aatcgcaacg tctgcacaat
    27001 aatagggatt tttagcaatt tttttgaaca tcgtctgatt ttctggtaat gcaaataaat
    27061 aaatcggcca ttgttcagga ttttcgaaag aatcctttaa gtacgtatca acagcttggt
    27121 aatagttgac ccaatcgatc tctacttctt catcttttgg atttacgcca tggaaagtta
    27181 ctccatctcg ggctccgcct cctccattag aagagaagtt caaaccgcca cctgtcagtt
    27241 cagatcccaa tgcagtaacg acatcaaccg gtgccccttc tggtagagat acttctttta
    27301 cttgtttgtt gctgacctca tacaatttca tggagtcgcg gttcaatccc aataaataat
    27361 aagaatagtt gaactgccca tttttgataa tagccagtaa ataaggaaga tcacttacat
    27421 agtattgatc gtctaccggg atactcaaac gatggatata ggtctggtca gcagttataa
    27481 tgaccgctac actcgtagtt gcactacgcc aaaacgaagg atctcctaac aaattttcaa
    27541 ttttttcttg gaacggagac cagtcatgtt cggaatattt tttctcaaaa cgtttctttg
    27601 cttctttcgc aaaatttttg aatttgatct gatctttttc cacatcttga tgggcgacat
    27661 gcgtattcag catgatcgtg acgaatggtc ctttagcgcc ttctgaagtc aattgtgtca
    27721 ataattcttt atcctttttt gccataaacg tacccctcct aaaaaaatca ttcctttgtt
    27781 tctatctaaa gtctaccaca acactatttt tccatacaat aaaacgtatc taaaaaaata
    27841 aaacggtttc tacggtttta aatttagcta tttttttaag ttatcttcat aatttcctca
    27901 attatttagc cccagtcttc aactattctt catgaaatgc tgataaacta agaaaaagaa
    27961 taaaagaggt ggatcttatg catttaatta tgattttcag tttattaagt attatcagtt
    28021 tatttgcttt cagcgcttcc cgcttattag atgcggaaac acggcctatt cctgtaaaag
    28081 tacgtaaaaa cgaccgctaa acttccataa cgcgcgttct tatcctaaag ttttttcaga
    28141 aacattgaaa ctctctcccc tctctgataa aattttaatt tgtagcgaaa caaattaatt
    28201 acaaggagat cggatatgtc cataaaaaaa acacgtctag tcctattatt ggctagtctc
    28261 attctttgcc ttgcttcttt tctaccagct gttgctgtgc aagctgaaga tacgttcaaa
    28321 gtaaatgcaa aagcagcttt tgctgtagat gcgcaaagcg gtaagattct ttatgatcaa
    28381 gatggagaaa aaaccatggg gatcgcttca atcacaaaaa tcatcggtct ttacatcgtc
    28441 cttgatcaag taaaagaagg aaaactgtca tgggatgata aagtttcgat ttcagattat
    28501 gcagaaaatt tgagtatcac cccagattta tcgaatgttc ctttgcataa agaaaacacc
    28561 tacactgtca aagaattatt cgactctgca atcatccagt cagccaatgc ttcaatggtg
    28621 gccttggcag aaaaaatctc tggcagcgaa gctaaattca ctgaacgaat gaaagaacaa
    28681 ttgaaagatt ggggaatcaa agatgcaaca atcgttaatg catcaggatt gaacaactct
    28741 tatcttggcg aaaaccgacc agaaggcact ggagaaaacg acgaaaatca aatgtctgct
    28801 caagatgtgg ccatcgttgc ccgccatttg atcttggatt tcccagaaat actggatgta
    28861 tcaagtacta cgacccaaat gtttggcgaa aatacgcaat caccagtgga aatggtcaac
    28921 tggaactgga tgctcccagg attcgttaat tacaaggaag gcgtggacgg attaaaaact
    28981 ggtacgactg agcttgcagg tgcttgtttc gttggtacga tcactaaaaa cgatcaacgg
    29041 atcatcactg tcgtattgaa tgcggacggc catgcggaaa acccgagtgc ccgcttcaat
    29101 gaaacagacc gtttgatgga ctactgctat gacaattggt cagaaaaaga gctcggaaaa
    29161 gctaacgcaa gtattcctag tctaaaaacc attgatgtaa aagacggaaa agaatcaaca
    29221 gttcctgttg tgcttaaaag ccctgtaaaa gtatgggtac gaaacgatat ggatactggc
    29281 aaattaacga tcacgccaac acttgacaag aagcaagtca ctgacaatga gatccaagca
    29341 cctatggata aaggtacaaa aatcggtacc gctacgatca ccttggcaga cgataaatta
    29401 ggctatttag aagacaacaa tgcaccatca acagagatca ttacggataa aacagtggaa
    29461 aaagcgaata tattcgtgat tggctggcga cgcgtcgcag aatttttcga caacttgttt
    29521 taattgaaca ataagaaaaa catctttcca tcggaaagat gtttttttgc tttttctagt
    29581 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
    29641 tatgaatcta accagagatc tgatcttttt agttctctat cttattgggg tattctctat
    29701 cgtttattgg cgaaaacaga agaaggatcc ttccttgttc ttttacggca gtacgtggac
    29761 atttacggta atcttaactt attttgtcgt gttggaaatt ttttattcga ccttttattt
    29821 tttgattcca ttgttgttct ttagtatctt tgctttttct tatttcacag aaaaacgaag
    29881 gctgctgaat ggtcttttat tcaatgtttt cctcatcagt ttcggcatct acttgtttgt
    29941 cttgctgtat gaaacgcaaa atattttcct tggcggactg atcgccttga tcacgattcc
    30001 attgctactt gtccttttat ttggtattta tggattgatt gtttttcttt tttggaacgg
    30061 ggtcacagtt ttacgtagag aatcgcattc attagctaat cttttaacgt tgatactagc
    30121 catttttctt acactgtttc ttgtttttga ctttttcttg ttaaaatatt tgccgcaatg
    30181 gataaatgct ttgtttttct gcgttcccct gatcttgatc tatctattta tcgtattcta
    30241 caactttttg actgtctcat tcttgtatca gttcaaccgg cctcgataca atcaagactt
    30301 catcgttgtg ttaggcgctg ggttgatcaa tggagaaaca gtttctcctc ttctagccaa
    30361 gcgaatcaat aaggcaattg ctttttatcg ggctcaaagc cgtgctacat taaatccgcc
    30421 catcttattg atgtctggcg ggcaaggagc agacgaaaaa gtaccagagg ccattgcgat
    30481 gaaacaatat gccatggaac aaggaatccc tgaaagagac attcttgtcg aaaccaattc
    30541 gacaaccact ttggagaaca tgctctattc aaaggaaatc atggatcagc aaatgaaagg
    30601 gccataccgc gcgatcttta gttccaacaa ttaccacatt tttagagccg gtctttatgc
    30661 gtggcaagcc aaattgaaag caaatggtat tggtgcaaaa acagctttct actacttgcc
    30721 taatgcgttt ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat
    30781 catttgcgga ttgcttttcg caggctccgc attcttatcg ctgattactt tacttttcta
    30841 aaaaaacaag gtgtgacaaa acatatgtca caccttgttt ttttctttca ttctcttaac
    30901 tattggcaaa aagagtatcc cttttttgac cgctacttgc tgggcagaat aaataccgct
    30961 gttgcctgaa cacataaagc tgatcaagca gatcataaag aagtatactg ccgcttgtga
    31021 accgaacaat tcgatcccca tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa
    31081 tactccgata aagcccaatc ctgcaagaaa aggaacagaa acatgaagca aaggcgctag
    31141 tgctgcacca agcgttgcac caatttcaaa caatggtgtc acttctcccc cttgatatcc
    31201 cgcgcctaaa gacaaaactg tgaagaataa cttcccaata aaatcataga catgtgcctg
    31261 tccattaaat gattcttgaa gtaacggaag actcaatccg agataacgtt gtgtttgcaa
    31321 aatcaatgct gctgccacaa ctacagctcc acctatcgta tttcgccaaa caggatgagg
    31381 aatccattgt gcatatcgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat
    31441 caaaccaaaa gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg
    31501 aatcacaccc atttggtaat gtagatgaga aacaccatag ctttctgtca tgaaatttgc
    31561 aaacagtgca gcaaagaagc tagggaagat cgcttctgtt cggactttcc cgattgccaa
    31621 cacttctaat ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc
    31681 actgattcca ctgataatca agatttcccg ttctgcttta tccaatcgga acaaacgtgc
    31741 tgtattatct gctaacgatc cccccatttg gactgctgtt ccttcacggc caacagaccc
    31801 cccaaataaa tgggtagtta tggttccaaa cagtgtaagt gtaataagcc gcaacgggat
    31861 tttttcttct ccaccatttc cttgatcgat cactaagttg tttccacgac tagaaagacc
    31921 gccgtgatac gcatagagat aagcaaaaag cgcaccacta actggtagta gataaaggag
    31981 ccaaggatgt gatagtcgga tatctgtcac aactgtcagg ctatttaaga aaaaagcaga
    32041 caagctcccc atgaatatac cgagaatcgc tgtgatcacc agccacttta gcatgtacac
    32101 taaaatgcgc attggactcg gtaattgttt ttccataaaa aattcctcct caataaaaaa
    32161 acgcctacta aagaaagggg ctgtccccag actttagtag gcgtcattag ctatctttcg
    32221 ttagcattta aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt
    32281 ctgtaaaaaa caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg
    32341 agccaatttc gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt
    32401 tttcgtatac gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg acacattcga
    32461 tgttttctga cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat
    32521 cccttttaat ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt
    32581 ccagccgatg tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt
    32641 ttttactttt cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctgaac
    32701 gatttgcttc agcttctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa
    32761 aactgtcgtt aaaaatgtat cgatttctgg cagttcgtga tttaatatgt ctcgaatctt
    32821 ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc
    32881 atttaataac tgttctactt ctctttttat aaaaaaatat tggtatggtt ttattgtttg
    32941 ttccatgatt ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat
    33001 cttctgcaaa gtatgtgtta cctgaagatc ccggactctt ttaaataaga tacgttttgc
    33061 atcttatcat cacccccttt aatgcttatt cttctatgta catgattcat tcgataaata
    33121 aaagtaaacc gtatcttgaa cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa
    33181 agtcgtgtca aagtcccttg tttcgaatga atggtgttca aaagcttgca tctgcggtaa
    33241 taagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    33301 aagctgacca cttctgtcac aacctcaata tggtctatta ttggttttac ctgttaaatc
    33361 gaggattctt tcccaaagta acgttgccat ctcttacgca tacttgcaga catgggctga
    33421 gccgcttctt tgactgcgca gtatttgtcc acgaatgtca caatatatcc ttctttatac
    33481 ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa tgatatctcg ctcaagatcc
    33541 gacaaatcgg tgattttttc tgcattcttt acagcaattc ttggatggat atacgcatgt
    33601 gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga acagatcatg caaaagtcca
    33661 gcacgcgctg ttgctttagc attgccaccc catttttttg cgagcagata actatagtaa
    33721 gaaacactga tggaatgctc caaacgagtg gaatgaacat gctgtgtgta attcgctaat
    33781 tttttcactg cttctgtttc taaaagatct tgtacataag acatatattc ttcgtcttca
    33841 cgccattttt ctgtcataat tatcgatctg ccaccttttt gaatttcatt ttcttctgaa
    33901 attgatctaa gtataccacc agtgttcgga ttattctagt cgctctagat aaaatagcta
    33961 aagcttaata gttccttaat cataaagaaa aatgataaag aacgatccct gctgcgactc
    34021 cgacattcaa tgattccgca tttcctttca ttgggatata tacgttctga tcagccgaag
    34081 acaagatttc ctgacttact ccctgacctt cgtttcccat gattatagcg aaatcctctg
    34141 ttttttctac tttgtctaat gcatgtgctt cttcatttaa ttctgtaccg tacaccgtaa
    34201 accctttgcc tttcattaca gggatcactt ctgccaaatc tttttgtaac acaggcaaat
    34261 gataattgct tccctgcatg cttcgcagca cctttgtact ataaatatct gccgttcctt
    34321 tacctaataa aacaccagcc ataccaaacg catctgccgt acggatcatc gttcccacat
    34381 ttccaggatc ctgtacatta tctagtaaaa gccagcctcc tgaaaaatct ggcgtatctt
    34441 cagatgttaa cgtcatcact gctaagatcc cttgcggtgt aggtagatct gacatagcgg
    34501 tcatgacctc atctgaaact agaaaagtag aaatctcttt gtttacagcc agccagtctc
    34561 cccattcttt catacctctc tgtgtcacaa aaagttcttt tatttctgca tcagacgacg
    34621 cagcttcttc gatcagatgg aacccttcca aaagatattc tgccttttct tctctgtatt
    34681 tctttttatg tagttttttt aattctttga ttcttggatt ttttactgat tggatctctt
    34741 tcacattcct cacctcaact gattatatca tggaataggt gcttgagctt gtttcatcct
    34801 aaaaaaatcg gtatcattga tttataaaac aggaaagaaa ggtgacgaac atgcgcaaag
    34861 ttaaaatgaa tgtccaagga cgtgtccagg gcgtgggatt tcgctatatg accaagatgg
    34921 ttgctgatca gttaggcgtg accggaacgg taaaaaacga agacgacggc tcagtcacaa
    34981 tcgaagcaat cggaaatgac gacatcatcc aaaaatttat cgaagaagtc aagaagtctc
    35041 cgagcccaag tggacgagtg cagtatgtgg atgtccaaga agatccgatg attgaggaac
    35101 gaaagaaatt tgatgtggtg ggataaggtt gtttttgaag cgctttgcct cgagtattaa
    35161 ggagaaacat tttccaaata gcttctcata ttttgaaatg taatgactta ataccgagag
    35221 gcagctgata aaacaccgtc aataaggttg tgaaaaagct ggtctgcatc aagcattaag
    35281 gactggttgt gaaaaatagc tctccatatt tttcaacagg ctggacttaa tgccgtagat
    35341 gcaagctttt gaacaccgtc gataaggttg cgaaaaagct gatctgcatc aagcattaag
    35401 gactagttgt gaaaaagtcg cttagttttg agtattaagg aaaattttgt gaaaatggct
    35461 ttccacattt ttgcataaat ttgacttaat accaagaaac tgacttttga acatcgtcga
    35521 taaggttgtt tttgaggcac tttgcctcaa gtattaaaga caggtagtaa aaaagctgat
    35581 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    35641 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttctaagt atttctttct
    35701 tgattttttg tcttttttca aaatttttcg ctacgaaaaa gaacgaatat ttatatgaaa
    35761 tttctatgaa gttgattgaa ttccatgcgc ttttttagta tagttaagtt tgtgtaaaaa
    35821 ttatagaaga ggaagtattc tatgaataaa tggaaaaact ggttattggg atccggactt
    35881 gtcagtatcc tgctcttttt atctggctgt gtgcggatgg attcaaacgg aaaccctgac
    35941 acttcaggaa tcgtttatcg agtactcgtc catcctatgg gccaagcgat tacatatcta
    36001 gttgagaatt tcaattggtc ttacggctgg gcagttattg caatgactgt gatcgttcgt
    36061 atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa caatgatcca atctgaaaaa
    36121 atgcaagcgt taaaaccaca agttgaagca gcacaacaaa agctaaaagc tgcaacaaca
    36181 cgtgaagaac aaatggcagc acaagcagaa atgcagcaag tatatcgcga aaatggattg
    36241 agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc aaatgccgat cttctctgcc
    36301 ttgtatttta cggctcgcta cacagaaggt atccgtgaat catcttttta cggcatcgat
    36361 ctaggctcac caagtatggt gcttgtggcc atcgcagggg tcgcttatct gcttcaagga
    36421 tatatttcaa caatcggtat tcctgaagaa cagaaaaaga cgatgcgtac gatgctgatc
    36481 gtctctcctg ctatgatcgt cttcatgtcg atcagtgcgc cagccggtgt tacactttac
    36541 tgggtagtcg gtggtgtgtt cagctgtctg caaacattca tcacaaacgt tataatgaaa
    36601 ccgcgtttga aagcacaagt ggcagaagag ttgaaacaaa acccgccaaa acaagtcgtt
    36661 actccaagaa aagacgtgac agaatcagca aagccagctg acaacaaaca agcaacaaaa
    36721 caaatcaaac aaccaaaaaa cagtacaaac aacaaaggcc gcaacgcagg aaagcagcag
    36781 aagcggaaat aaaaatatat gacaaaaaac tgtagacaaa taaaaaaatt tctttgttct
    36841 acagtttttt tgt
//
LOCUS       DACCCR010000022        36564 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCCR010000022 DACCCR010000000
VERSION     DACCCR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.022"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IVK93_001093"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IVK93_001093"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP6386397.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IVK93_001094"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IVK93_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP6386398.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IVK93_001095"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IVK93_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP6386399.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IVK93_001096"
     CDS             complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IVK93_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP6386400.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IVK93_001097"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IVK93_001097"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP6386401.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IVK93_001098"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IVK93_001098"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP6386402.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="IVK93_001099"
     CDS             complement(8052..8522)
                     /locus_tag="IVK93_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP6386403.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="IVK93_001100"
     CDS             complement(8568..8993)
                     /locus_tag="IVK93_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP6386404.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="IVK93_001101"
     CDS             complement(9060..9329)
                     /locus_tag="IVK93_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP6386405.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="IVK93_001102"
     CDS             complement(9340..10935)
                     /locus_tag="IVK93_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6386406.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IVK93_001103"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IVK93_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP6386407.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="IVK93_001104"
     CDS             complement(14488..15048)
                     /locus_tag="IVK93_001104"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP6386408.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="IVK93_001105"
     CDS             15401..16375
                     /locus_tag="IVK93_001105"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6386409.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="IVK93_001106"
     CDS             16545..17945
                     /locus_tag="IVK93_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP6386410.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="IVK93_001107"
     CDS             complement(18023..18745)
                     /locus_tag="IVK93_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6386411.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="IVK93_001108"
     CDS             complement(18824..19330)
                     /locus_tag="IVK93_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6386412.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="IVK93_001109"
     CDS             19453..21252
                     /locus_tag="IVK93_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP6386413.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="IVK93_001110"
     CDS             complement(21320..22894)
                     /locus_tag="IVK93_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386414.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="IVK93_001111"
     CDS             complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="IVK93_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP6386415.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="IVK93_001112"
     CDS             complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="IVK93_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP6386416.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24671..25309)
                     /locus_tag="IVK93_001113"
     CDS             complement(24671..25309)
                     /locus_tag="IVK93_001113"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP6386417.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25310..25960)
                     /locus_tag="IVK93_001114"
     CDS             complement(25310..25960)
                     /locus_tag="IVK93_001114"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP6386418.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="IVK93_001115"
     CDS             complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="IVK93_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP6386419.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="IVK93_001116"
     CDS             complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="IVK93_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP6386420.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29103..29843)
                     /locus_tag="IVK93_001117"
     CDS             complement(29103..29843)
                     /locus_tag="IVK93_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP6386421.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="IVK93_001118"
     CDS             complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="IVK93_001118"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP6386422.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31214..32152)
                     /locus_tag="IVK93_001119"
     CDS             complement(31214..32152)
                     /locus_tag="IVK93_001119"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP6386423.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32145..32636)
                     /gene="def"
                     /locus_tag="IVK93_001120"
     CDS             complement(32145..32636)
                     /gene="def"
                     /locus_tag="IVK93_001120"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6386424.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32651..35059)
                     /gene="priA"
                     /locus_tag="IVK93_001121"
     CDS             complement(32651..35059)
                     /gene="priA"
                     /locus_tag="IVK93_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP6386425.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35312..35617)
                     /locus_tag="IVK93_001122"
     CDS             complement(35312..35617)
                     /locus_tag="IVK93_001122"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP6386426.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="IVK93_001123"
     CDS             complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="IVK93_001123"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP6386427.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9883 a         7987 c         6706 g        11988 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttatgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggcttaatt
    22921 taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt accatagcag
    22981 tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt taaaaatgac
    23041 aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag catttcttaa
    23101 gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt attcatcact
    23161 gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca acagcagtgt
    23221 aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt taaaaatcaa
    23281 ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac tgaaaaattc
    23341 agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata caattaaagg
    23401 gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga tagccccgag
    23461 aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac ataataatgc
    23521 caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc ctagcattct
    23581 tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat agtacttcat
    23641 tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg tcacaactga
    23701 cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc cataactatc
    23761 tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc ctccaagacc
    23821 ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt gggtcaccac
    23881 taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc cattagccga
    23941 agatacccgt actggaaaat gattggagct ttcattagag gatgacagta attcgatttt
    24001 agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg acatgactgc
    24061 catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt cctgccaact
    24121 gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca gcgtctgatt
    24181 caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt tttgctgtga
    24241 aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat aagttgaaaa
    24301 aagattatgg acagaatcta ctccatttcg tacccaccgt actggggcag agattgtttt
    24361 atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct ttccttcatt
    24421 cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa taaccaaggt
    24481 aataataata tttttattag gattgaactt cttcacaaag acacctcgaa ttaattagac
    24541 tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc tcctcttaag
    24601 ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa tttttttatt
    24661 tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa aaagaaaatt
    24721 ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct acttctttat
    24781 tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc aagcagcaat
    24841 aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc aaataataag
    24901 acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg aagcgaggtt
    24961 ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta gctccaatca
    25021 aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac tgtgtatctg
    25081 tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat acttccttct
    25141 gctcttcgtt cgacaatgaa tcaaaatcgc ccactgccag atctactggg tagcctgctt
    25201 gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg gacagcagag
    25261 actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt tagtctaatg
    25321 cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata tatgatcctg
    25381 caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga acgattccgc
    25441 catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg agttccgtaa
    25501 ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg acagtcatga
    25561 ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt gttcctggat
    25621 tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc cgatgaatat
    25681 gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca aatgctggaa
    25741 tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta gtaacaggac
    25801 ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaattgg ccatccatta
    25861 catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct ctttgtaaat
    25921 tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc tcctattttc
    25981 gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt aagaattgca
    26041 aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt tttacttcgc
    26101 agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa gcttctacaa
    26161 attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca atcgagctaa
    26221 atcctggtgt atctgctact aacccgccat agataggaat cagttccaca tgacgtgttg
    26281 tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc agttccggtg
    26341 atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag accgatagcc
    26401 gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt acaatcaccg
    26461 gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg tcgtctgaca
    26521 agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag gtaactagaa
    26581 agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca atgattccaa
    26641 gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag atttccagaa
    26701 gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca agcggcgtga
    26761 ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg tttgcgtata
    26821 catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc acctccgttt
    26881 ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac ttctcaaagt
    26941 atatggcttg aaaattgatc aaaagagata gaaacacaaa aaagagaatg caacaaatca
    27001 atgtcgcatt cccttctgat atattttatt cagcacttat tttgaacttt ctgaggtgct
    27061 agctgatgaa cttgtggagc tggaagattc ttcttttgaa gagttgcttg tgctactgga
    27121 gctacttgag ctgctagaac tgtctgaaga gtctttttca gaactagaag acgctggatc
    27181 tggtccttta gagtaaatta cgttcacaac cattcctttg gtaattgtcg ttcctgcccc
    27241 tggtactgta cgaatcactt tatctttatc caccgtgttt gaatattcgg aagtctcatg
    27301 cccgatatat tctgcaccga cactagctaa ataactttgt gcttcgcttt ttgtatagcc
    27361 ggagatatca ttcaatttag gctctgttcc ttggcttacg acaaaattga tgtaagtttc
    27421 ttctgctaca acttcacccg aggctggatc ctgactgatg atcgaacctg atggtacagt
    27481 gtcgctatag tcataagtag cactaatatt attgatattg aatccttgtg taacaagcca
    27541 attacgaaca gtttcgtagt tttctcctac atgactactc aaagtcactg tttctggtcc
    27601 tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg acttctgtat cttctgatgg
    27661 agattgcgaa atgatcattc cgctttctac ctctgaactg aattcctctt ctttttgaat
    27721 caagtcagca gaaaacccta attctttcaa acttgcttcg gcatcttcat aacttttacc
    27781 actgtaatca tctaatttga tttttttcgt gccgctacta atatacagag tgacttcgcg
    27841 tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc actacattac cttcttcgat
    27901 tttgtcatct gcaatttctt ctgtttttgt tgctacttct aatttcgctt ttgcgagtgt
    27961 ttctctagca gaagcttcag aaagatcaga aacatcagga acaacaactt ctcctcgccc
    28021 gccagaagca aagaatacag ctgttccgat tcctaaaaca gcaagaatag ctaataggat
    28081 cagccaccat tttttctgtc ctttcttttt aggatcaggc agaacttctt cctcctctgg
    28141 caaggtttcg ttttctggct tgtcttttgg cagaggcata gatttgaatg aatcaggcat
    28201 tggtgcatct tccgtaaggg gtgtgataac ttttgtttca ttatccatca catgaggctg
    28261 ccatttttct tcgtttgcac gagctggtga taatactgta gaaagatcat cagacatttc
    28321 ctcggctgac ttataacggt ctgcaggttc tttagccgtt gcccgtaaaa tcacgttttc
    28381 cagtgcttgt ggcacatttg gatcaagtgc ttttatagat ggaagatcat cttgaaaatg
    28441 ttttaaagca attgttacag cagattcacc atcaaacggt acgctacccg tcaacatctc
    28501 ataaaggatg atccctaaag cataaatatc tgactgcttc gttgccatac ttcctctggc
    28561 ctgctcaggg gacagataat gaacagatcc cagcatcgta ttcgtctgcg tgatcgatgt
    28621 ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt acaacacctt cattatcgat
    28681 cagtacgttt tggggtttca aatctcggtg gatgatttga tggttatgag ctaaagaaat
    28741 agcagaaagg atctgctgca taatatttac tgctgtttca taaggaacag gataatgtgt
    28801 ttggatatat cgtttcaagt ccattccttt gacgtattcc atcaccagat actgcatgtt
    28861 gtcttcttca ccaacatcgt aaacactaac aatgtttgga tgaacgagtt ctgttgctgc
    28921 taaagcttct ctctgaaatc gtcgaatcgc agtctggtca ttttgaaagt caaatcgtaa
    28981 gactttaact gctacatcac gatccaagat taaatcgtgc gccaaaaata catttgccat
    29041 accaccgcta ccgatatttc cagtgatttg ataacggcca tttaattttt tgcccaactc
    29101 gatcatacac tgtcctcctt atattcgata agaagaacgg tgatattatc cgctccgcct
    29161 gcttcattgg cacgatcaat cagctcagtt actttatgag acaatgggcc ttcttgattg
    29221 atgatcgttc ctatttcttc ttcagacagc atgttcgtca agccatctga acataacagc
    29281 agtcgatctt ttagttgcca aagatgagta gaaacatcta cttcgactgt tccaggcatg
    29341 ccgacagaac gtgttaaaat atttttcctt ggatggttca ctgccatttc ttcgctgatt
    29401 tctcctgatt tgactagttc attgactaaa gaatgatcct ccgtcaattg catgatttta
    29461 tcttcacgaa tcaaataagc tctgctatcc ccaacattgg caatcgtgaa ccagtcatct
    29521 gacagagcgg ccgctacgat tgttgttccc ataccgttat attccggctg ttcttgtcca
    29581 cgctgataaa tcagcatatt ttcttcttga atcgattgaa tgaaccattg ggcgattttt
    29641 tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta gattcgttac cgccatctga
    29701 ctggcaatgt ctccggcacg atgaccaccc atcccgtctg ctaatacagc aagcttgaga
    29761 cctgaacggc tggtaaatac actggcatag tcttgatttg tgttacgtcg tcttcctaca
    29821 tctgattgat attcaatctg cattttttca cctcattact ttttgcgcaa acaacaaatg
    29881 aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca acattttctc ttcgattgat
    29941 gattgtagca taggattggt caaaacatca atcctctcaa aatcagggtg tctttccaag
    30001 aaagcagaaa caacgtcttg gttttcttct tttaagatcg tacaagtgct ataagtcaga
    30061 ataccggaag actttaaagt tggtgcgcag ctttctaaaa tttccaattg gatttttggt
    30121 aaattcgcta attcatccgg ctgtttttta taacgaatat ctggttttcg ccgaagcagc
    30181 ccaagacctg aacaaggcgc gtctactaaa atccgatcaa actgctctgc ttcgaactgt
    30241 ttactgactt ctctagcatc catcttttga gcgtagacag cagaagagac acctagacgt
    30301 gctgcattat cttcaatgag tttgactttg tgttcgtgta catctaaagc gaccacttct
    30361 ccaccgcatg acggatctaa aaaagaagca atatgcgtcg tcttacctcc gggtgctgca
    30421 caggcatcta agacatgatg ttcaggctcg atttgcatag acggcgctac aagcatcgaa
    30481 ctttcatctt ggatcgtcat gacgccatcg tggaacaatg agctagaagc taaatgccct
    30541 tttttcgcta ctataccgta tggactgact tggctttttt cagctgtgat ctcttcttct
    30601 tgcaaaacag tgatggcctg atctctggaa agtcggcgtg tatctactcg ggcactagca
    30661 tggctaggct gatatagcga taaaccgagc ttttcagttt cttcgtatcc gatttgatcc
    30721 accagtcgtt tcgtcatcca tagaggcagg ctgattttga cagaaagtct ttcgatagga
    30781 tcagcaatgc cttgcagatc tggtgctcct tgacgctgat aattcctcaa gacaccattt
    30841 acaaatttcc ctgtcccagg atttcctttt acttttgcga tttcgaccgc ctcgtttaaa
    30901 atagcatgtg cagggacttt atctaaaaat tctaattggt atacagaaag ataaagaagc
    30961 gtcttgaccc agtcatccac tttttttgct ttagcgatca atggtgcaac gtaaaattcc
    31021 aatagtcgct gatggctaat cgtaccatag atcaattctg taaataaacc cacatctttt
    31081 tgatttagct cgctttgttt gatcatctca ttcaacaaaa gattcgagta ggcgccaccc
    31141 ttgttcactc gctctaatgt cagtaatgcc atatatcgta ctgatttttt cattttttta
    31201 gggatttttt tcattatttc acctgctgac caatctgaac ttgctgaccg cttccgttaa
    31261 ggaaatcatg tacggcctgt tttcctttac cagctggctg taattcattg atggctaaga
    31321 cggtctgttt accgcaagca atccatagat tttttttgtc tttccggatg atcgtaccag
    31381 gtgctttgtc tgtagtttct gccaatactt caaccgataa taatttccag cgtatgccct
    31441 cataggtcgt gaatgcaatt ggccaaggac gcattccgcg tacttggttg tcaatttctt
    31501 ctgcagtttt atgccaatcg attgcttctt gttcccgtgt gatgtttggc gaaaaagttg
    31561 cttctgactc gtcttgaggt actggttttt gtccatccaa aatattgggc aatgtttcta
    31621 gcaatagctt tcttcctaaa aggctaagct tttcaaacat cgtccctaca tcatcttgtt
    31681 tagtgattgg tatgctttct tgcgcataga ttccgcctgc atccattttt ttgatcattt
    31741 ccatgatcgt tacaccagtt tctttctccc cattcatgat ggcataatga acaggcgcac
    31801 ctccccgata tttaggaagt aaagaagcat ggacattgat tgcccctaat tttggtactt
    31861 gcaataactt ttcaggcaag aattggccaa atgccgcagt gacgatcaca tcaggcacca
    31921 attcaatgat ttcttccatt tctggcgaac cggagatttt ctctggctgc agcacacgga
    31981 taccgtgctt cagagccgct tcttttaccg gtgtcggcgt aattactcgt ttccgcccaa
    32041 ctggacggtc gggctgtgtt accactgcct ggatctcgta acctgattct atcagccctt
    32101 caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac tagcttagtc atcaagatgt
    32161 tcctccatat attcttcaag ttcttcttcc gggatctgct gtatcatttt ctcaatgaac
    32221 aagataccgt ctaagtgatc gatttcatgc tggaaggcac gggctaaata gccaaaagcc
    32281 gtcacttcta tctcttctcc gtctcgatca taataacgca cggtcacttc atctgctcgc
    32341 ttgaccgttc catagacatg cggaatactg agacaacctt ctacgtcaag gctttcacct
    32401 tttgcttcga tgatttctgg gttaatcagt tcaaatttct cgccttcatc tacttcgatc
    32461 acagcaattc tcttgttttg tcctacttga ggagcagcga tccctattcc gtcattcgca
    32521 atcattgtct catataaatt gtctaataaa gcgattgtct cgtccgtgat tacatcgatt
    32581 ggctgagcag ttctttttaa tttatcatta gggtggatca atataggata gcgcataatt
    32641 tggccctttc ttaaataaag tttaatggtt cgttgtcaat tgccacaaaa agaccttgtc
    32701 gctgttcttt ctggcttcca ttcaaaattt catccaaaac atgaggaaga ttcggttcat
    32761 gtttatattt gatgattagt tgataataat atcggttttt cactctcaaa atcatgctgg
    32821 gcgttggccc taatagcaag ctttgagggc ttaacacttc ttttagctga ttggcaatct
    32881 gaaagatttt ctttgccgct acttgttctt caggatggct ggcggttatt ttcacagtaa
    32941 agtaataagg aggatagcct ccacgatgcc gtacatgcat ttcctgctga taaaactgct
    33001 cataattttg ttttttagct aattctatgg cgtaatgttg aggattgaat gtttggatca
    33061 cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga aacttgggta agcaattgga
    33121 atgtatgctc actagaacga aaatccggta aattcaggga ggtatcagca ttcaacaccc
    33181 caactaatgt cacttctgga aagtccaaac ctttagcaat catttgtgtc cctaataata
    33241 tatctgcttc ttttgcaccg aatttttgta aaatctgttc gtgtgcccct tttcttcgag
    33301 tggtatctac atccatacgc aaaatcctag cttgcggata aagttctcgg agttcctctt
    33361 ctactttttg agtacctgtt ccatagtagc ggattttatt gccgccacaa ttaggacagc
    33421 gattgggaat aggttcttca tgtccgcaat aatggcagcg cattgaacgt gtatccatat
    33481 gcagtgtaag agagatatcg caattaggac atggaagaac aaatccacag tccctacaca
    33541 tcacaaaaga cgaatatcct cgacgattga gcagtaggac agtttgttct tttttgtcta
    33601 atcgattttg tattttttcc tgcaaattag cagaaaaagt agaggtccga tgattctgaa
    33661 actcttcacg catatctacg atctcaatag ctggaagctg tgcagctgct ttggctcggt
    33721 ggtttaactg caataactga taaacgcctt tttgagctcg tgctcttgat tctaaagagg
    33781 gcgtcgcgct tcctagaact attgggcaat gatgatattt actgcgccaa attgctagat
    33841 ctctcgcatg ataacgcggt gtctcatctt gtttgtaact tgattcatgt tcttcatcga
    33901 taatgatcag cccgatattt ttcagcgggg caaaaatagc agatcgtgcg cctactacta
    33961 cttgcgcttc ttcccgctca atcttgcgcc attcatcata tttttcacct tgggaaagtc
    34021 cgctatgtaa gaccgcaact gattctccga agcgtccttt gaatcgctcg accatttgcg
    34081 gagtcaatgc gatttcaggc actaacataa tagccgtttt attttctgac aaaacatcag
    34141 cgattgcctg aagatatact tctgttttcc cgctgccagt gattccctcc agcaaaaaga
    34201 cctgatcttt ttgctgctga cctgctgtga tgatttgttc gacagcattt ttttgttctg
    34261 cattcagttc caatgccgtc gtttggtcaa atactcggtc tttataaggg tcgcgataac
    34321 gttctgattc tataaactcc agccagccct tttcttttcc ttgattcaag acagcagtac
    34381 tgattccttt atttttgaaa taagcaattg gttcttcttg gctcagttca gataaacaca
    34441 aaagaagttc tttttgtttc ttggcatttt gccgtaattt tgcccattct tcttcgattt
    34501 gcttttcagt taacaaagaa cggaccaaac gaatcgtttt tactttattt cgtgtatgga
    34561 cttcatattt gatatctact aattgctctt ggcgccagcg aatcaattga gaaaggctgc
    34621 cttcttcttc tgcttccttc caggaaatca cttctcgctg atcaaacaat acctgaactt
    34681 ctggtgtgtc tgccaaagga tagatcaatt tatcatattc tgctttcatc acactcggaa
    34741 gcatcgtttg tagacaagtg attttgaatg cgtaagtaat ttttttcata taatcagcta
    34801 atgaaagtaa ttcttcattg accaccggag caaggtctag caaacgaata actgccttca
    34861 aagatggatt agctgattct tctgattttt ggatagccat aacaaatcct tggacatgac
    34921 gattgccatt accaaaaggg acttctactc gcatcccgac ttgcaaagct gtttccattt
    34981 cagagggaac caaataagtg aatggctgat cggtctgcat agtaggtacg tccacaatga
    35041 cttcagctgt taatgtcatg gttcgtttcc tccttttttt gtcttttctt atcaagcgac
    35101 aaaaagttcc ttgcctattt tactagagat cgatggattt gtcttatctt ctgccaaatc
    35161 ctaacaaaaa attaacagca gataagaaaa aaaagaactg tgtcaaaaag ttcagacaca
    35221 gccttttcta ccagacaata agtggcgaat gtttgaaaag gaaacagctg ggtttgcttt
    35281 tctttttaga tgttcgctct atttttcttt attacatttt ttgctcttca cggatacgtg
    35341 attccaattc gtgttgttca cgctcttttt gtgcttgacg ttcctcttct tccatcttca
    35401 aacgttcacg ttttaattca ggatgtggat cattgactac atttcctgca tcgatttctt
    35461 ctaaagcttg accgacgctt tttactgaat caaaagaatc tagagtaggc tgtgcacttg
    35521 catccaattc atgcgcgcgt ttacttgcta aaatcactag agaatatttt gaatttacac
    35581 gatctaatag tgagtcaatt gaaggtttta acatcataag actacatctc ctttaacatt
    35641 ttaatatatt taccgattac gcgatctact cgaaaatgtt cactggcaat aatgtttttg
    35701 attcgttcaa ctgctagcgg cacttcatca tttacaacag catagtcgta aagagccatc
    35761 atttcgattt cttcttttgc aactttcatt cgttcttcga ttacttcatc tgcatctgtt
    35821 cctcggccta cgatccgtga ttttaattcc gctaaatctg gcggagtcaa aaagatgaat
    35881 acaccatcgg gcactttttc tttgacttgt tttgctcctt ggacttcaat ctccaagaaa
    35941 acatcttttc cttcatccaa tgtcttgttg atatatgaca aaggagttcc ataataattc
    36001 ccgacatatt ctgcgtattc cagcatctct ccagcttcga tcattgattc aaactcttct
    36061 ttgcttcgga agaaataatc aacgccttcg acctcccctt cacgcatttt acgggtagtc
    36121 attgagatcg aatactgaaa atcattatcc tcactctcaa aaattgcttt tcgtactgtg
    36181 ccttttccta caccggatgg accagacaac acgattaata atccacgctc tgacatgacg
    36241 acgtcctttc aactagtctt tttgtactta tcgtttattt tgcacttttt ttctgcaact
    36301 ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa agctaccctg taccaagagg
    36361 tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacataaac agcttctcat
    36421 gtttttcgtt tggttggggt tattatcgca gatgcaagct tttgaacgcc gtgtataaga
    36481 ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa ttttgtgaaa atagtttctc
    36541 acatttttgc ataaatttgg ctta
//
LOCUS       DACCCR010000023        36264 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCCR010000023 DACCCR010000000
VERSION     DACCCR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.023"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(42..2720)
                     /locus_tag="IVK93_001124"
                     /pseudo
     CDS             complement(42..2720)
                     /locus_tag="IVK93_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2707..2925)
                     /locus_tag="IVK93_001125"
     CDS             complement(2707..2925)
                     /locus_tag="IVK93_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386428.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(3001..4053)
                     /locus_tag="IVK93_001126"
     CDS             complement(3001..4053)
                     /locus_tag="IVK93_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6386429.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(4206..4532)
                     /locus_tag="IVK93_001127"
     CDS             complement(4206..4532)
                     /locus_tag="IVK93_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386430.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(4544..4744)
                     /locus_tag="IVK93_001128"
     CDS             complement(4544..4744)
                     /locus_tag="IVK93_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6386431.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            4819..5469
                     /locus_tag="IVK93_001129"
     CDS             4819..5469
                     /locus_tag="IVK93_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6386432.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            5524..6690
                     /locus_tag="IVK93_001130"
     CDS             5524..6690
                     /locus_tag="IVK93_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6386433.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(6779..7171)
                     /gene="rpsI"
                     /locus_tag="IVK93_001131"
     CDS             complement(6779..7171)
                     /gene="rpsI"
                     /locus_tag="IVK93_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6386434.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(7187..7630)
                     /gene="rplM"
                     /locus_tag="IVK93_001132"
     CDS             complement(7187..7630)
                     /gene="rplM"
                     /locus_tag="IVK93_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP6386435.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(8149..8616)
                     /locus_tag="IVK93_001133"
     CDS             complement(8149..8616)
                     /locus_tag="IVK93_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP6386436.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8736..10274)
                     /locus_tag="IVK93_001134"
     CDS             complement(8736..10274)
                     /locus_tag="IVK93_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP6386437.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            10354..11040
                     /locus_tag="IVK93_001135"
     CDS             10354..11040
                     /locus_tag="IVK93_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP6386438.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(11241..11768)
                     /gene="msrA"
                     /locus_tag="IVK93_001136"
     CDS             complement(11241..11768)
                     /gene="msrA"
                     /locus_tag="IVK93_001136"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6386439.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11852..12529)
                     /gene="pgmB"
                     /locus_tag="IVK93_001137"
     CDS             complement(11852..12529)
                     /gene="pgmB"
                     /locus_tag="IVK93_001137"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6386440.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(12526..13656)
                     /locus_tag="IVK93_001138"
     CDS             complement(12526..13656)
                     /locus_tag="IVK93_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6386441.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13656..15839)
                     /locus_tag="IVK93_001139"
     CDS             complement(13656..15839)
                     /locus_tag="IVK93_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6386442.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15836..16861)
                     /locus_tag="IVK93_001140"
     CDS             complement(15836..16861)
                     /locus_tag="IVK93_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6386443.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16906..17733)
                     /locus_tag="IVK93_001141"
     CDS             complement(16906..17733)
                     /locus_tag="IVK93_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6386444.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17730..18797)
                     /locus_tag="IVK93_001142"
     CDS             complement(17730..18797)
                     /locus_tag="IVK93_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP6386445.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18823..19659)
                     /locus_tag="IVK93_001143"
     CDS             complement(18823..19659)
                     /locus_tag="IVK93_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6386446.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19656..20525)
                     /locus_tag="IVK93_001144"
     CDS             complement(19656..20525)
                     /locus_tag="IVK93_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6386447.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(20595..21878)
                     /locus_tag="IVK93_001145"
     CDS             complement(20595..21878)
                     /locus_tag="IVK93_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6386448.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21892..23577)
                     /locus_tag="IVK93_001146"
     CDS             complement(21892..23577)
                     /locus_tag="IVK93_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP6386449.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23852..24877
                     /locus_tag="IVK93_001147"
     CDS             23852..24877
                     /locus_tag="IVK93_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6386450.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(25472..29125)
                     /gene="rpoC"
                     /locus_tag="IVK93_001148"
     CDS             complement(25472..29125)
                     /gene="rpoC"
                     /locus_tag="IVK93_001148"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP6386451.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(29204..32821)
                     /gene="rpoB"
                     /locus_tag="IVK93_001149"
     CDS             complement(29204..32821)
                     /gene="rpoB"
                     /locus_tag="IVK93_001149"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6386452.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            33549..34517
                     /locus_tag="IVK93_001150"
     CDS             33549..34517
                     /locus_tag="IVK93_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6386453.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            34535..35440
                     /locus_tag="IVK93_001151"
     CDS             34535..35440
                     /locus_tag="IVK93_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6386454.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            35437..36243
                     /locus_tag="IVK93_001152"
     CDS             35437..36243
                     /locus_tag="IVK93_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6386455.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        10273 a         7632 c         6508 g        11851 t
ORIGIN      
        1 ttcgtcaagt tctaagaaat catgcgtact aatctttaga attaattgca tgacaatccc
       61 ccccttttat ttcttaataa attatcgaaa agtggattag gatcattaag tgtatctctg
      121 aataactcat tgtcttcata agtgaatctt cgtcttgatt ttgatccctt atcattttca
      181 cggatttcac tatcaataat cactgtttca tgatttgaat caaatttttt cgctgacttt
      241 ctgtcttcaa cgatattgat tttttgtgtt ttttctttca tttctccact atctttttga
      301 atgttctccc gaacatctac tgttttgaat ccttgactac ttgtagaact ttgtacattt
      361 tctcggacat ctactgtttt tggttgactt gcactgctac cttgcacatt ctctcggaca
      421 tccactgatt taaacccttg actacttgta gaattttgta cattttctcg aacatctact
      481 gtttttggtt gacttgtact gctactttgc agattttctc gaacatcaat tgtcttagta
      541 ccttgagcat ttgaagaact ttgtaaattc tctcttacat caacattccg tccaacatga
      601 ccattcagtg aaccttgaat attttctcgg acatctactg atcggccacc atgactactt
      661 gtagaatttt gtggattttc tttgactaat ttttctaatc ctgtatttga atttttagct
      721 tcttgaattc tttctttggt caacgcttct tttccgtcaa tattagcgcc tgtccgccct
      781 tctacacgtt tccttaacat tatttcctcg cgattcattg cttcaggcgt gtatttcgct
      841 tcatgacgct tctgattaat taattcttca gcagacttag cttgaccgtg catagcattt
      901 ttacgttctt caatatcttg ttggactttt tgctgaattg cgcttgcagg attattattc
      961 gattcagcat tatttttcat tgcttcacgc atacgttcag aactagacat aattctatcg
     1021 gcattttcaa ctttagcacc atcttttcta tctgaaagac gtgttggcac ggaatcagca
     1081 tattttccag tttcatcttc tttattttta gctaacatag gtttgctatt tttattaata
     1141 gcggacactg taccatcatc taaaccttct ttaaacttat tcttggtatc attagctata
     1201 tcacgcatac tcttaaatgg ttttgttgct ctttcactag ccattgtagc tccatctgaa
     1261 gctaaaccgg ataatccacg ttcatgggca tatcccaaag ctcttccagt tttgccagct
     1321 ttttttgata gagtttcgcc gaaattcttt tctggttttc gatcttctcc ttctccgctt
     1381 cctttagcac gattaaatcc atttgatcct tttctaaaca gcattgcgcc cattccaaaa
     1441 gcagaagcaa gttgcccata gccttctttg agtccaatat caacaccaaa gtatctcatg
     1501 actgttccac tgcctttgat taaaacaaaa acggcacaga tataagcaat tgtttttata
     1561 aaaatatttg ttgagatacc ttgtcctgag ttcagaaaat tgataaacat agcaaacatt
     1621 gataacccaa atccttgaaa cccaacagtt aagtaacatt gtagaatatc tgatagaaca
     1681 acttttgaac gctgtcctgt ctcaatatct gttgcaaaaa caagaacacc taacacacgt
     1741 ttaaatgcaa gttctagtat tgctgtaatg atcacaaacg cgctgaacac ataggcgatt
     1801 gctaacgctg tcaacccaat tgaaatccct catagatttg cttggtatcg gtaatagcct
     1861 gatttcaagc tatcagaaaa tgttgataag aagttgtcag aaaactttgt agcaacaaat
     1921 tcattattag aattttcaac taattcataa cgaagattgt ccgcaattgg attttctttt
     1981 tccaatttat caatcacttt atccgttaac acttgtgtga gatccgtctg ttgaaaattt
     2041 tttcgtgtta aattatttcg atcctccgtt ttatcaatgc ttgagtactg atctgtttta
     2101 ttaatatatg ctaaatctgt tactccttgt tttatcaatg accaagcgac tccgtcatct
     2161 gaatttgtaa tcgtagtaga gtccttataa aatgttttag caaattgaat accactagaa
     2221 atcatggttg gcattaaaag tattaatgct atagacatca ctacgttcat tccaaccgat
     2281 tttaaatcca ctgttccttt tccagtaatc attttgtaac caacaaacat caatgaaagg
     2341 atcattaatc caatgacaat tgtatttacg gctgattgat aaacattatt tagccctgta
     2401 ctttcaatga aattgaacaa atccagcaca tctggaatca tattttgaat cgaatctgtc
     2461 acagtaaaca tgccttttac cacagcccat ccgagccaaa tcccgaactg actaaggaaa
     2521 tctccattgt acttttttag gtaaggtcta taaagagaat tattcaaaaa ctctgtttga
     2581 ctttttgtgt catcaaacca attgaaaata ctcgcatgag caatattact tgtaaaaata
     2641 atcatagcta tcagaacaag aaaattaaca accaaccaat ttattttttt attggcaatc
     2701 tttttttcaa ttattttcat cgttacctcc cttagcccat tttctgaggc tctcagagag
     2761 cttctgagcc attttttgat taattatgac atgagttaca caattatcat tttcagctct
     2821 taaatcgaga actatagtgt tgaagcaaat agtctcgatt aattcgagac tatctgtatt
     2881 ttgatctaga caccaaactt gatctattaa acgattttta atcaaaataa cccctcctat
     2941 attcaaagtt ctcaaatgaa gtggagatac ccctccattt catttgactc aattctattc
     3001 ttattgctta tctattcctt cgctgatttg caattcaatt agttctaacg ctttattttt
     3061 tagagaagca aagatataat cgtctttttg tttgatacga tgatctgttt ttgctcttcg
     3121 tatcaagtca cgtaaagtca ttgatagctg ttctctaaca tgatctacaa aatacttgtt
     3181 attacaaatt tcatagataa ttttcagttg aattttttgt tctttttcag cagatctaat
     3241 cgcatatttg aattttttgt accagttgtc gaattgacga taatcactat tgctagccag
     3301 ttttaagcaa ttcacaatac tttctcccca tatttgttta agaagatttt cttcaataaa
     3361 tgtttcgatc aattcttcat ctaatttatt atttttagtt gaaagtgatt caagaagatc
     3421 atcattatgt gattctttaa tatctttact atctttataa gacttatata attctagttc
     3481 attttttgaa ctatcagcaa aattgttttt gtctcttgtt ttcattgatg tgtcgcatag
     3541 ttcaaatttt gaattatcaa cagaatattg atttgttcca tagttcaaat tttgaactat
     3601 caagtttttt tgtctatttt cttgatgggt tatcttatta attttataaa catcatcttt
     3661 gcacagaaca ggctttaaca aataataaaa atttgggaac atttgcattt tacttttatt
     3721 gaagccattt tttcttttca caagaagttt aactttttga agttcatcta gtgctgataa
     3781 gattgtattt tttgacattc ccgaaatttc tacgagattt ttgatttcaa atatcaaaaa
     3841 aattttttga ttctcatcat acaagttatt tttcaatgaa agtctatttc tatcttttag
     3901 taacatataa actaacttag aacttagttt cattttctta tatttttcat ttacgtttaa
     3961 aaaaatagga tattgatagt ataattctgc atatatatca gaattagaat agccttgctt
     4021 ttcaactaag ctttcaactt ctttttcatt cattgtcaca cctgcttctg attttatttg
     4081 ctataataga ttcaaataaa gttttctcag aactttatta ttgagtgttc ccccgatctg
     4141 caaactgacg ggaacactct ttttaatttt ttctacttca tcaaatttaa aaaacgaggt
     4201 gttttttaaa tttgatgact caatatccat tttttgcatt cttcttttga aaacaactcc
     4261 attttttcta ataaaataac tggcaatccc attcttttaa atttcaaaaa agttgtatat
     4321 gatacattca aatatttagc catctctttt ccttttagaa atggtctttc taatccggta
     4381 tcaattcttg cttgatcaat agcctctttt gtcatttcaa aaacataatt tttcaattga
     4441 ttcgtttgtt gtttatctaa cattactgta attcctgcat ccattatttt tctcctctca
     4501 ttttctattt cttcaatcag ccaactcgcc aagttgtatt tatttagatt ttcaaatcat
     4561 taagtaacca atttaccaac ttttgataag tagatgctct aatactttct ttgctcccat
     4621 tttcgatttt gacatatgtt ttccgattta tacctatcaa ttttgctgca tcgtttttag
     4681 taagtataag atcggctcta tgtcttctta agcttctaat gaattgatca ttaagagtta
     4741 tcattcagcg cctcctttca aattattcac tttgtggttt ttatcttaat aagcacattt
     4801 taaaccacaa aacgagtaat atcaattaaa atattactca atttgtggtt ttcatgctat
     4861 aatagtttgg aatttactat agggggcgtt actttgaata gaataaaatt ttatattgat
     4921 tccagtaaaa agaccattaa acaaattagt acagaaactg gtattaacta tacaactctt
     4981 acgaactatt accgtggtga gcgaaatcct agaaatactg aaacatggaa tatccttgct
     5041 gattatttcc atgttcctgt aagttatttg atgggattag acgaaacaaa ttccatagaa
     5101 caacctaatc ttgatagaat aaaaactgct cgtttagaaa aaaaggacaa aatagtcaag
     5161 aatattgcca gtcttgactc agattatttt actgaattat ctaatctata cttttccatg
     5221 acttctttac ttgaagtcaa acttcaagaa gatgactccg actttctttt tttaaaaagt
     5281 attctagagg actcctttga atacacgatt ctatttgcca aacaaaagaa gtttattaat
     5341 aaagctttaa acttaaatat cgatgaaaga aaagagttag aatctgagtt atttggcaac
     5401 ttaacccaag tacaggttaa caatctaaaa agagagataa cagctcgatt aatatcaaaa
     5461 cttcaataaa actaattatt caatcagcca actcgccaag ttatcagatt ggagagatta
     5521 gttatgtcaa tacaaaagta cacaacaaaa agcggtgcac gatatagtgt ccgcttgtat
     5581 gttcgtaaag atgaaaatgg aaagcaagaa tactatgtaa aaaaagggtt taaaactgaa
     5641 aaagaagcca agttacacga agcaagaaaa aaggtcgaga ttgaagatac cggctatcat
     5701 aagccttcta atgacttgtt tataacagtg tatgaagaat ggttgccttc ttataggaat
     5761 cgtgtacaag aaacgacata tcaacgtaca aaagaccttt tccgcttgca tatactacca
     5821 aaattcggga ataagtccat ttctaagatt actcctgttc actgtcagaa tgcagtcaat
     5881 gaatgggctt taaaattcaa gaatatcaaa cagcttaagt cttacagctc tcaaatattt
     5941 gagtatgcca tttttgcaga gctattacac cgaaatccta tggcaaatac aaatctacca
     6001 aagcgggaaa agaaagaaaa tgaaaattac ttttcattag aagaactaaa agctttttgg
     6061 gatattctca ataaagaaga gcctttaaaa catattttaa tcttccagct tttgattact
     6121 actggtatac gtaaaggaga gctagctgct cttcattggt ctgacattta ttttgaagaa
     6181 cagctaatgt atgttagaaa gagctatgca actattcgaa atgaagagaa gaatgcaaga
     6241 agaaaaactg tcaggattca aaaaaataca aaaaatatat cttccgaacg aatattacca
     6301 attgatgcac aaactattga aatgttaaaa atctggaaaa aagaacaagc tcgagagctt
     6361 ttgcagtttg gcataaatac aaatacaaaa aatcaactta tctttaccta tgtaaatgca
     6421 gacggtgaca ttaatcaacc tttgcatgcg gactattcaa ataatatatt gaaacgttta
     6481 gagaagaaat ataaatttaa gcacgttact attcatggat tccggcacac tcacgctact
     6541 cttctactag aaggtggagc aagtatcaaa gaaacacaag atcgattggg tcataaaaat
     6601 gctgaaacta cccttaacac ttattctcat gttactgaaa aagctcaacg aaatgcgtta
     6661 gaaaaattca ttgcttacac tggtttataa tattgtcatt acctttctca ttaccagaac
     6721 gaaaaaaaga caccttctcg caatgagaaa gtgtctgaaa tcgttggtat aacaacgatt
     6781 aacgttttga aaactgtgaa gctttacgtg ctttcttaag acctggtttt ttacgttcga
     6841 ccatacgtgc gtcacgagta agtaagccag cacgtttaag agctaaacgg aagtcaggat
     6901 caacttctag caatgcacga gcgataccat gacggatagc tcctgattgt ccagcgtatc
     6961 ctccgccgtt tacgttaaca aatacgtcat atgcgccttt tgtttcagta acagcgaaag
     7021 gttgattgat tacttcacgc aagtcagcat gtggaatgta ttcttcaaca tcttttttat
     7081 ttacagtaat tttgccagtt cctggtacta gacgtacgcg agcaactgcg tttttacgac
     7141 ggcctgtgcc gatatattga acttgtgcca atgaaattcc ctcctattag attaagtttg
     7201 tgatgtctaa aacttctggt tgttgtgctg catgtggatg ttctgctcca ccgtatacgt
     7261 tcaattttgt aaattgtttg cggcctaaag tgtttttagg aagcatacct tttacagaag
     7321 tttcgatcaa acgacgagaa tttttagcac gcaattcgcc agcagtgatt gatttcaatc
     7381 ctcctgggta gttgctgtga cggtaataaa ttttgtcagt cgctttttta cctgttaatt
     7441 tcactttgtc tgcattgatg acgatgacga aatcgccagt atccaaatga ggtgtgaaag
     7501 ttggtttatt tttaccacgt agtacagatg caacaactgt tgaaagacgt cccaatggaa
     7561 catcagttgc gtctactaca taccatttac gttctacttc gcctggtttg gccatatatg
     7621 ttgtacgcac gttattttcc tccaatttaa gttctgatgt ttgacataca cttcgagttt
     7681 ccggggctct ttgtggggca aacaatacca tttaacatat tacccgctaa tctagtcaat
     7741 gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac tttacttctt ttctttcttt
     7801 tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa agaggctgtg acaaaagtct
     7861 tgtcacaacc ccttattttg aataaacggt gttctcctgc caactcctcg gtcacaagtc
     7921 acaatatcca taaaacataa gaagctgttt tttaaaattg tttcttgtga ctcggagctg
     7981 aacggcaggc ttacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc
     8041 tcaaaaaacg gcaaaatatg aggaactatt ttgacgtttg tttctctaat actcaggtca
     8101 aaacgctgat ttcacaacct ctttttcggt caagttcaac aacagttttt acgcgtcgat
     8161 ctctggcgct ttgcccaaat gagcttggat catccagata cgtttttctg tggctgtttt
     8221 gaattcgata aagatatctt gtgttggatc gtcgccttct tctccagaaa cttcgattcc
     8281 tttttggtaa agatctgcta gataacgata gcctgcaaca agtgtttcca aacgttctgg
     8341 aatagtacga tcccagcggc caatttcatc agggattttt gtgttatctg caaattcacg
     8401 caatgtagaa taaggtgaac cgtctaatgt gatcaagcgt tcggatactt catctaactg
     8461 agcgttcaaa tcatccatga actcatccat cattgggtgc aatgtaagga aacctggtcc
     8521 tctcatgtac caatgtgttt ggtgaacaac aacagacatt tggcttagat ctgcaactaa
     8581 ttgatttaat acttctttgg tttgttggaa tttcatcttt cttgcctcct aacattttct
     8641 tacacttaaa ataatagtag actttcaatg aaaagtacaa caaaatcatt tacctgagaa
     8701 cgattttcag ttcgcttttt tatccgtaaa gccaatcatt tttcagaatc agctaattct
     8761 tctcgcagtt gagcaaattg ctgataagtc tgtgctaaat gcttttgtgt cgaaacaaat
     8821 aatggataga ttttctgata ttttttatga tcagtttctt gtactccaac ttgctctttc
     8881 aattctatca attcctgtgc ttcactgata ctttggatct ttccgatgct tctccagcct
     8941 aaaatcacag ctcctaatgc ggatgcttct gtgacagtag ggaaaacaag cgggtaaccc
     9001 agtacatcag ccatcatctg acgaaaaatc ggagattgag caaacccgcc tgtagcacgg
     9061 atctctgtga gctgattttc ctggcaaact ccttttaaaa tccgataaag gttgagacag
     9121 atcccttcta gaatcgcctt catcatttct ttttctgtat gttgccgctg taaaccgata
     9181 aaactgccaa acgcatctgc gtcccaaagc ggtgctcgct caccaagtaa atagggatga
     9241 aaaatcagac cattggcacc cgctggcaca aaagcgatag ccgaaagcag tgcatcatat
     9301 ggattttctc ctttttcttt agcagattga acgacttcct gtaaaaatgt atgccctgcc
     9361 caatcaaaga tgccagcacc attgctagtc gctcctccca ctacccaatg tttctgatcc
     9421 agaacataac aaaacgtctc catttctgga tggaactgag gctgagtagt aataaaacga
     9481 agtgctccgc tcgttcctac ggtcaaagcg gcgatcgtat caccaaaagc acctaaccct
     9541 agattagaaa gcggcccgtc tgcccctccc aaaacaaatg atatatcatt tggcaaacct
     9601 agttctgctg ctgtttttga tggtagacct gttacttgat aggtggaagg aaagacttgc
     9661 ggcaattgct ctattcgaat gttcaagtag tcaagcactt cttctgccca ttggaattca
     9721 tggatatcaa aatatccagt tcctgaagct gttccgtagt cgctgatcag ttcgccaaac
     9781 aatctataaa agatatagtc tttgataccg acaaaataag cagttttttt gaataattca
     9841 ggttggctgt tttgcagcca cttgattttg ttcaatggtg tcattggatg aatcggcgtc
     9901 ccagtctgcc gataaaattc tttgccaagc acggtatatt tcagtttttc tgcttcgtcc
     9961 gctgctcggt tatctgccca agtgatcatt cgagataacg gtgttttctc ttcatccaac
    10021 aatatcaggc catgcatcgc agaggagaag gagacaaatt gaatgattgt gtctgaaggc
    10081 aactgtttaa ttacttcttt tataacggtg cagacacctt gaaagatcaa ttcctgatct
    10141 tgttctgcca tcccttgtgt ttcttggatc aaagggtagc gaaaataagc ggatgctgct
    10201 tcttgggctt catctgtgaa agcaactgct ttggtttgtg tggttccgat atctaatccg
    10261 attgaaacgt tcattttttc acctcaaatt ttatgtatgt acaaaaattt agtttaagct
    10321 tgcgtatctt ggttttgaaa agggggagat ggcgtgttac tttattttat cattggttgc
    10381 tgtatcggat catttttatg tttaaccgcc cagcggattc cgttaggtca ctctattatt
    10441 tatcctagat ctcattgtgt caaatgctgt cgttcgcttt cttggtatga attgattccg
    10501 attctctcga tcatcgtcca acgatttcgc tgccgctatt gcaggtgtcg gttgcccttc
    10561 tactatttgt tggcagaagc cttatgtggt ggtctttttg cctggttctt tactttttct
    10621 tccgcaagaa atttttcgac gttcatatgg atgctttcgg ctttgctatt ttcgttgatg
    10681 gatttatttt acttcatggt ccattctcac acgctattct gttcttgggc aattttatgg
    10741 attttttggt tgcaggccgg cgtcttccaa tggcaaagtg tgctgttaac gtttatcgta
    10801 ggcggtgtgt gccttcgcta tggtcaatcc tatctcggag ctggcgatac tctgttgatg
    10861 ctttcttgga gtggtggctt gtctctagaa gagttgcttc agatcttgtt tcttgctagt
    10921 ctgtttggaa tatccgtttt ttctgtttac tttatctgtc ataaaaaaaa gttggaaaag
    10981 ctgcctttca ttcctttttt gagcagtgcg ttgctgattg tccttcatct taggtattga
    11041 aaaaaggttg tgacaaacga cttgtcacaa cctctgatat atagtgttct cttaccaaat
    11101 ccttgtgact tggagctagg cgacaggttc acaacctctc cactaaatgg tgttcaatca
    11161 gctgaccctc agtattagct caaaatgctg gttttacaac ctctgatata cggtgttcca
    11221 taagtagctt tttcggcttc ttatttcttg acgccccaat gtcgacgaag gaaacctgca
    11281 cgtccagaac cttctcgata aagattgtaa tggagcttgt ttttcttgta ataatcctga
    11341 tggtaatctt ctgcaggata aaattcgctt cttggttcga tttttgtcac gattggctta
    11401 tcgaatcgcc cgctgtcttg caatgcttgt tttgaacgct ctgcaatatt tcgttcttct
    11461 tcatcattat agtagatcac tggacgataa gaatcaccgc gatcagcgaa ctgtcctaat
    11521 gcgtcagtcg gatcggtttg ctgccaatag atttccacca actgatcata agaaataact
    11581 tctggatcat attcgatctc tactgcttct gtatgccctg ttgtgcctgt tgttacttgt
    11641 tcataagttg gatttggtac atgcccgcca gtataaccgg atgtcacaga aatgatccct
    11701 ggctgctctt caaatggttt gaccatacac caaaagcatc cgcctgcgaa aactgctttt
    11761 tttgtcatat aaaatccccc ttttttctca tcataccttg atttccaaaa ggaggataac
    11821 gttttgcttg tgacagaaaa aacggaaact attatatacg tgatacggct tctgataaat
    11881 gccaaaacga acgtacttga tcaaaaacgg ttaaatcagg aatcacatat tcagcagaag
    11941 gaagatttat gttttcacct attcccaata catgcatgtt accagccaaa gctgcttgac
    12001 aaccagcttc tgaatcttca atgaccaagc atgcatttgg cggtacattc aattgctgtg
    12061 ctcctttgag aaaaacttct ggatcaggtt ttgcttttga tacttgcgtg ccgtcaataa
    12121 ggacatcaaa ataacttgtc aaattcgttt tttctaaaat gaggcgtgca tttttgcttg
    12181 ctgatcccaa tccgatcttg atgtttgttt ttttcaagta ctcaagaaca gctattgcac
    12241 caggcaaaac ggatgtttca tccatttttt gaatcgcttg aagatataga ttgttttttt
    12301 gctcagcaaa ggctgatttc tctttttctg agaatttctg ttccattttt ccaaatgaca
    12361 gcaatcgctc tagtgactcg gttctggata ttcctttcag ttgctcgttt tctttctcag
    12421 tgaatggaat ggacagctgg ttagctaaaa ccagccacgc ttcgtaatga taacgagctg
    12481 tgtctacaag aacgccatct aaatcgaata gtgctgcttt gattttcatc gattacctcc
    12541 ttgaaaatga acgacttgaa gtaaagatgc gatgttttta gctgtgcgat gaagattatc
    12601 agtggcttgc tgtaaagctt cttgtaaagg cacagctgca ggaaggatcg gaaaaacggc
    12661 cgaaatgcca acatctgctg ctttttcgct tcctttacca acacttccgc aaatagctat
    12721 cacaggaacg ccctctggta ctcttctagc aatcccaact ggcgcttttc cttttagact
    12781 ttgtccatct agacgtcctt cgcctacgat tacgaggtct gcttgctgta cccgtttatc
    12841 gaactccagt aaatccaata cgtagtcgat gccagagact attttccctg aagcaaatgc
    12901 taacagtcct gcacccattc caccaccagc actactgccc gatgcctcga ttacttgagg
    12961 ggcaaatctc atataaaaat catgcatggc ttggtcagcc actgctaatt cattttcttt
    13021 caagcctttt tgtggtccga aaacaaaaga agcgccttct tttccgcata aaagattagt
    13081 aacatcagta atgacagtga ttttggcttt aatcagctca ataggcacgt tcgttttatc
    13141 aactgtagca attttaccta gattttctcc cagtgcttct actttttgat tatcttgatc
    13201 gtaaaaagca taacctagac cttctgccat gccgatgccg ccatcgttgg tactacttcc
    13261 gccaacgccg atgatgatgt gtcgaacacc ttgttgcaac aattgtcgta tcagttctcc
    13321 tactcctcgc gtggataacc taagtggctg ttgttggttt agtgggacat ctttcaatcc
    13381 gacgatttct gccatttcaa aaatcgcagt ttgaccgtct gaagcgtact ggatttcttt
    13441 gtttttgcca aatgcattag taacgactgc tgtttgccgc ttcaacctta aactgtctgc
    13501 taatgatgaa accgttcctt ctcctccatc gcctataggt agcagatcat attcagcctg
    13561 aacaaaaact tcttgaaaac ctgattgaat ggcttgtgct gcctctaagg ctgtcagact
    13621 ttctttgaaa gaatctggcg caatcagaat acgcattatt ttcccctccg ttccaagatc
    13681 agttcttttt ccagctggta ttggtctcca ttgacaaaaa taggtaatgt ttctttcgtc
    13741 tcttttttta ttactatttg tttttttgtc aaaacaaata gcaacctgtg atttttccaa
    13801 ataaaaggaa aagaaatttt ttcccatgct gaaggaagtc ttggagaaat agaaagagtt
    13861 ccattcgtta ctgtaatacc tgaaaaacca aatataactg ctagccatag tgctcctaat
    13921 gaagcagcat gaattccgtc atctgatgaa tgcggatctg gtcctaaatc aatcaggcac
    13981 gcttcttgga ataaatgata cgcccattcc atttcttgga tacggcaagc ctctatggcg
    14041 tgaatcgctt tactcaacga tgagtcgtgg attgttttgt tttcatagta atgcaaattc
    14101 ttagaaacga ttggttgaga aaaatactca ggtaataagt ataacagcat gacaagatct
    14161 gcttgcttgc tgacttggag ttcatttact tcgctacggg cataatcaag taaaatagac
    14221 tggctgccag gacgttcttt gtaagtggtc aaatcaataa ctggcttact gagatacgtg
    14281 tcatcttgcg gaatcagctg ttctttgtta ggcactggca aatataactt atctaaaaaa
    14341 ttttgacatt ctttcaaaaa aagcggatcg ccagcttgat atttaacagc taaggccaca
    14401 ttataatgcg ccagatagtt cgtatatgca ttattgtcga tatattctgt gtattcatct
    14461 ggaccaatga ctctgtgtat ctctaaatga tcatcgactt ttacagctct gctcagccaa
    14521 aaagctgctg tttcatgaat gagcgttttt ccctttgttt ctaaaaacga ttgatcctct
    14581 gttgcttgga catactcttg aacggcataa gcaaggtctg cactgatatg atgttctgcg
    14641 gcagctgaag caacttcttg tcttgttccg gttttgatat tcaatgcagc aaatttgggt
    14701 gtctcttcct gaccagtgtg tgcgctttcc catgggaaaa gtgcaccttg atagtgataa
    14761 tctcttgctt tttgccgtgc gccttctaat cgatcataac ggtacagcag caaattcttt
    14821 gctacttcag gaaactgata taaaaagaaa ggcaggataa aaatttccgt atcccagaaa
    14881 acatgccctt tataaccttc gccagttagt cccttcgccc ctacactaac acttggatca
    14941 ttgcggggag tcataatctg taaatggtaa gcagcgaagt ccattgctaa ctgatcgaaa
    15001 gaagaatcgc cttggatcgt tatccgatgc tgatgccaaa aatctgtcca ataagacatc
    15061 gactgctcta aaatttctgt ataactaacg cttttctggg cttgggcaat acttttctta
    15121 aacgggtctg tttgctgaag agaggtagag agcacgatga tcttctccag cgtaaaggac
    15181 gtattttttc tgatcttttc tgaaaaggaa gcggtgatcc ggcgattttt tgcttggaat
    15241 acacctgctt tattgaattt agctgcaatc ccaatatcaa ttttactttc tgttgtctgg
    15301 taggatgcac tgatcaaatc gtcaccaaat actcgcagtt ctttttcaac caactgctgt
    15361 gtaccaaaat tggtttgctg tccatcgata cccgttttca catctacttg gcagtcttcg
    15421 gtcaacggct tgatgaccac tttcagggct gcctgatgga gattattttg atcagcaaca
    15481 cgacaagtaa tgacttccac tttttttcct tgagcagttt caatgagcgc atagcgttta
    15541 agctctccca tttctaaatc tagtgcccgc tcgtatgcaa gaactttcat cgtctcaaga
    15601 gaaaaatgtt caccgtcgat ttttatttcc catctgatga catctgggag attcactaac
    15661 tcggctgatt ctgctcctaa aggtcggtta tatacaccag ctgcaaacat tccccttact
    15721 tgattccgat agtcttcctc ctggcttcct ctgataccaa aaaatccatt tccaatggtc
    15781 atctgactcg catacttatt gatatactct aaatcaaact gtttttcttc tactttcata
    15841 aggtaataac ttctcctgtt tctgccgaac gatataaatt ttccaccaga ttctggatga
    15901 catatccttg ccagccatct gcaatcatct gagtctggtc accgagcgcc cgattcacaa
    15961 aatgagccat acttagttca tgtgggtcat taactggcgt ggtcttttgt aaggaaacca
    16021 gctttccatc tacatcttcg aatagttcgc ctggatacag attagcacct gcctgatcgc
    16081 cacaaaaagc cacattcagt ttgtcttctc tgatattcaa agcaaacgaa gtattcagtt
    16141 ggatgatctt gccgttttct aattccagtg tgccaaaaat tgcatcttct actgtgaatt
    16201 tgcttggatc ccatttcccg aatttccctg aatttttttt cgtaccaatt ttttggaagc
    16261 tgtacgcccg aacgcttttt actttaggaa atccagtaat atacatcgct gtatcaagca
    16321 tgtgaatgcc ataatcgatc aaaggcccgc ctccttgaag ttctttgtcg ataaacgttc
    16381 cccagcctgg aacaccagac cgacgaagcg cattcgcttc tacgtaataa atatccccga
    16441 gaacatcaag atgattcttg atcattgttg cttcgtcaga aaaacgatgc tggaatccat
    16501 aagccaaaat ctgcccgttt ttatcggctg tctctgccat ctttcttgct tgatcagctg
    16561 tcatagctgg tggtttctcg cacatcactg aagcattagc ttcgagggca gccaatacat
    16621 gttcgaagtg aaagcgattg ggtgtacaga cactcacgat atcgggagaa atctcattca
    16681 gcatttgtgt aactgatgta tacgcatgag aaaatcctgt acgtcggcaa aagtcttctg
    16741 ctcgctggct gactggatca accacagcgg ccaattctaa gacatctgat cttttttgat
    16801 aataagcagc atgtactttt tctgctactt gtccagcgcc tatgattgct actttcttca
    16861 tttatttctc tctcctttgc ttatttactt attgttattc tttctctatt ctttctctaa
    16921 atcatttaaa tgacgcgctg tttccagaca gtttttcaag tattgcaatg attcctgata
    16981 aattttcact tcattttctc ctcgcaaacg acattcatat accatataac cttcatactc
    17041 attttgaagt agcgtttgaa aatggcgagt aaaatcaatt gcgccagatc ctggttggta
    17101 gcgatgatta tctgctaaat ggatatgacc cacatactct ttttgcgtag tcaatgcttt
    17161 tgaaatatca tcttcttcaa tattcatatg ataaaaatca gcgatgatct gcacatgttt
    17221 caattgatta gagtcaatgt aatgacgcgc atccgctaac aaattgatca tatggtcttg
    17281 gtatcggttc aaaggctcca aataaataac cgtacctgtt tcttcagcta ctttatctaa
    17341 ataacgaagt gattcgctga ctgctttata gtctccttct ttgcttcggg gtgaaaccat
    17401 cggcggtaat cgataagtga acattcccca agctgctggg ataacgattc ctttgcctcc
    17461 aacctcgctt agtgcacgca aaatctcagc aatttgacgt acaccattaa gacgccgttc
    17521 ttcgataaag tcaccgatcc acccatcgta gccgttacaa gctgtcacta ctgggatccc
    17581 tgtttctttg attgcctgtt tgactgtgtc gatattttct attaacaact taccgtcgat
    17641 ttcgtattct tcaaatccac agtctttgat aaatctaaat ttttctaaaa tgtctcttgg
    17701 gaaaaatgct tgatcttgcg taccgaattt cattgttctc ctcctcggac ttgaacgccc
    17761 attttgatac tttcttctgg gtgctggtct acataacgca tgtaagcttc tgcgctttct
    17821 gcaaatggca caactggatc aatgatttct tcacaatcca agtaaccgtt catcaataat
    17881 tcccaacatg tgtcctcgat tcgtttacgg ttccatcttg ggtaatctgg gtttggttca
    17941 ctagatgctc gagcaaagac gatttttgca ttattgaaat gtgcttcgcg accaagatta
    18001 aacccagctg gaaaaggttt agcaaaggca acataagaaa tgatcccgcc atagccgatc
    18061 cctattaaag aagcttgtaa tgcggcagca ctgccactcg tttcaataac tgaatctact
    18121 ccacgtttat ctgtcaattc ctttagacga aaaccgacat ctgctttcat aggatctagc
    18181 gtgtaatcgg caccgtgttt ttctgctaat tctctacgct gcgctatggg atcgatccca
    18241 acgatggtag tagcacctgc ttttttagct aattgaatcg caatttgccc gatagcgcct
    18301 aaaccgaaga ttgctacagc atcacctgct cgaacatgtg cttcccggat accacctaaa
    18361 gcgaattgag caggatcgta acatactgca tttttccatt gatccaaccg atcaagttta
    18421 cgtagtttat aattatcaac accgtttgta atcagcgttt cacgaatggg tccataagtg
    18481 cagactaact ctcctttttg atactctgtc accaaactgc ccgtttcttc gatcgtaccg
    18541 acaatcatat ttcctaattg aaattcgcca aataccacac cgcgttggct tccttcttct
    18601 cttggcaaga ataggcgcca ttcttctgaa aattcttcat cgataaatgg acttaatcct
    18661 ctaaagtcga caatttctgt tcctggtttt ggagtagcga aaattactcg gatacgtact
    18721 tcgttctttt cgatctcacg gtcttcgtaa tctttgaatc cggctattct tggttctaat
    18781 gcaactaatt ttttcatctt ttctttcctc tttcttttct ttttatcctt ttacgccgcc
    18841 ttcgactaaa tcacctttaa taaatcgttc agataacgca tacataatga tgactggtaa
    18901 agcggtcacc aatgaagcgg ccatcattcg tccccaaatg tagtctggtg tactgaataa
    18961 tgtgttcaaa ccgattggta aagtaaattt ttcggaggtc gacaggaaaa tcgaagcaaa
    19021 taaataatca ttccacgaaa ccataaagca ataaacgaag acagaaatga ttccagaaat
    19081 agccaatggt ataatgatcc gaataatgat ctggatccgg ctaagaccat cgatcattgc
    19141 tgcttcttca atatcagtcg gaatcgtgtc aaaataactt tttagcatga atatcgcagt
    19201 aggaagtgtt tgaacgacca tcgtgataat cagtgctgta ttggtatcat acaaacctag
    19261 accggaaatg attttaaata atggaacaac gatcaaaata ccggaaaaca tataaacggt
    19321 gtaaaagcta gcattgattt tcgagcgagc tttgaagcgt agttttgaca aagcgtatgc
    19381 cccgctgatc ccaatcacta ctgcagttac ggaggctatc aaggagatca ctaaactatt
    19441 tttgaaatac atgatgaatg gaaaaatctc agcattgaaa atatcctgat aatgttcaaa
    19501 agtccattct ttcggaaaaa tagtttgtgt cgttgcaatt gcttcttgtg aatccttgaa
    19561 tgatgtcatc agcatgatca aaaatggaaa caaattgaca gctaatagca acaaaatcgt
    19621 cgcgtaaaat aagattttcc tgctcttttt atttttcatt tcaagtccac cttctttcgt
    19681 gagagcaaga cgaccgctgc aataatgatg aacaatagaa tcgatatagc tgctgctttt
    19741 ccaagatcat tgaacgcaaa ggctgtctga tacaaataaa cgcctaagat attgacttta
    19801 ttcgtcagta gatagacatc tgtaaacata tagaacatcc agatagatcg taaaacaatc
    19861 acagttgcta gtactggttt gattgccggc agtgtcacga tcttgaactt ctgccaagca
    19921 tttgcaccgt ccatttcagc tgcttcataa agcgttttgt cgactgtttg caaaatagcc
    19981 aaaaatgaaa taaacgcata cggatagtat ctccagatag caaacaaaac gaccataaag
    20041 aagctagcaa ctggctggtc gaaccacagc ggtgcttcgt tgaaaagctg caacctatct
    20101 actaacagat cattgacgat tccataacta ttattgaaca tatatttcca agcaaaaatc
    20161 aaagaaatag atggcgtaac ataacttaat aaaatcagtg aacgcaccgt tttcctaaat
    20221 ctgaacttgc ggttaagcaa aatagctacg gctaatccta aagctgtact gcctgctaca
    20281 actaagaatg tgtaagcaac tgttaaacct aaagaacgat aaaattcact atcacttaaa
    20341 atttcaagat aattgcttaa tccgataaaa acggcgtctg agttaggatt cagcggcata
    20401 tcgaaaaaac tgatctgaat attggaaagc attggaatca gcactaaaac agctaataaa
    20461 agcatgcttg gtgctaaaag tcccatggct aattttaagt cagaatgttt ccttggacga
    20521 ttcattgaaa cctccccctg ataatctgcc aatagctatc tacttttttc gaacaccatt
    20581 ggcagaactt cttgttattt cgtatttttt tgtgcgtcag ctaaagcttg atcgacatct
    20641 tcgtttccga cagttacttg gttcactgct tttccgatca cacctgagct tgtgatatct
    20701 cccattttcg tgaaattctt ttccccgact agtccaaaga cttgtacttc atcaaaagca
    20761 ctagcaatct cagatggcag ctcgccaaaa gacttgatga cttcattttc tttgtaacca
    20821 tccagttcga ctacttgttt attcactggt tgagcaccac caggagacat caagacccac
    20881 ttttccatgt ttttcggttg agataagaaa gctgtgaatg cttcagcagc ctctttttgt
    20941 ttctcgtcta gaccagaaga aatcgtcaat ccagatactg tgccatagac agcttgatct
    21001 ttttgtgttg ggatagcaaa accgatattt tctgagttcc ccgcttcata aacagaaggc
    21061 aaaatgtaag ttgaatagat tgccattggt gctgttccat tcatgaaagc atctttgatt
    21121 tctgtcacat cattagagcc tggcatcgtg tattgggata agttttgata aaacgacaat
    21181 gcttctctca tcttttccgt atcgatcgtt acctcgcctt catcatccaa aacatttgct
    21241 ccatttgaca aagcaaattg agagaacgct tgctcactca ttgttccttc agcagtaggc
    21301 atagcaattc catattgttt attctcttta tctgtgaaat gttgagcaat ttttaaaata
    21361 tcatcccaat tttctggttc tgaaaaacca gcttctgata atttttcttt gtcataccaa
    21421 ataccttgaa cccagccgct aattggtgca gcaatatagc tttttccatc ttctgaacga
    21481 acaaggtttt tggctccgtc ataataatta tcttctccag cttcctcgat aacgttttgg
    21541 actgccgtct gatcaatcag ttcatcttta tccatcactt tagcaaaatc ctgactgact
    21601 tccattactt ctggtaattt accggaacga gctaacgtaa cgatttttgt attgaacgct
    21661 gattcttcta ccggcacttg cttgattttg atgtctggat tttctttttc aaaatcagca
    21721 accagttcat tgatcacact taatcgttcc tgttcgacag aagagtgcat gaattgaatc
    21781 gttgtctgtt gtgattcgtt agagctttga ctttcaccac aagcagtaag aagaaatcca
    21841 gtagctagca gacaggtagt aacaataatt tttttcattt ttttccacct cttattttat
    21901 tattttcagc cacatatatt catacggctg gaatatcaac tttcgttctt gactgcaatg
    21961 gataatttgt ccagaaaaca ggtctaggta atctcctttt ggtaaaagat aacctgctga
    22021 ttgattggaa aaattatgga taactaagac tttctcttcc ttcgcttcgc gaataaaact
    22081 gataaaagaa tctgctgagt ccagtaagta aatcaaacta ttcggatgga aggctttttc
    22141 tttttttcga atagatagca tctgggtcaa ttcaccatat actgctgttc ttaatggatc
    22201 tgtctgcaac tctttctcaa tatctgctaa caaaaatttt tgccgattga tttttcgatt
    22261 ctctcctgtt ttttctacgc ctttgtaatc atttcgactt cccagcacac tttgaatata
    22321 aattgccggc acgcctggta acgttaccaa aaatgaatga gcagctaaaa agcgtttgct
    22381 tctccattcc gtcgtatctg ttggagaact taacgcatct agatacgtag cgtttatctc
    22441 atatggactg gtcgttccat ctgggttttt cttgtagttt actcgggcac cctctttctt
    22501 cagctgttcc actaattcaa ggatcgtttt ttccggcaga atacctcgta ccggattcaa
    22561 tccgataccg tcatgggagg ctaaaaaatt aaaaaaagtt gtttttttcg atgcgaaatc
    22621 gatggttttt aaccattctt tcaacttact tgtattaccc gaacgaatcg cttgaagggt
    22681 caaaggtggt agcgaaaact gatagaccat ctgtgcttca ttctcgccat tcccaaaata
    22741 actgatatta tctttgtgtg gcacatttgt ctcagtgatc aataccgttc ctggagctgc
    22801 ttcatcagcg attgcacgga ataattggat gattttatgc gtttctggca ggtgtataca
    22861 agaagttcct agttctttcc ataaaaatcc cacagcgtcc aagcgaataa atgaagcgcc
    22921 tttttctaag taaaacaaaa gaatatcgat cattttcaat aaaacttctg gatttttaaa
    22981 attcaagtct acttgatctt cgctaaaagt cgtccatacg tgtttttctt tcccgttttt
    23041 gtcaatgaac gttgtaagta acgggctggt ccttggtcta gtcacgctgc ttgtatctgt
    23101 atccagtggc aaatcaataa aaaaatcttt gtacgtatca ttcaagttta aatattcctg
    23161 aaaccaagta ctttcggcag acatatgatt gcagacaaag tcgaacatta accgtgtttc
    23221 gcttctcatt tcttctatat cttcccatgt gcccaaacgg tcgttgattg cctgataatt
    23281 tacaacggag aaaccatcat cagatgtata tgggaaaaat ggcaagaaat gtacaactct
    23341 aaatgtatct gccaaatact tttgataaaa tttcttaaat gtacgcaaag ttggttgctg
    23401 ctcttcaaaa aattgatccc cataagtaat caagaaaata tcttcttcgt cccaatgcag
    23461 tttgcctgcc tggcctttct tctgctgata aacactgact ttttgttcaa tcatagaggc
    23521 ggttttctca gacaaatctg agtcgcgata aatcgcttgt aaaagttttg tatacatcgg
    23581 attccccttt cttttttggg agcgttccca acgatttgat catattatgg aaaacgctta
    23641 ctgtcaatag cattttttta aataattttg atatacacaa aaatacaaat aagaaagctt
    23701 atgtatcaat aattaatagc ttcttaaaaa aatatggtct ctcatgatca tgcaagctat
    23761 tcctaaattt tcaaaatcat agacaaaaaa gaatgtctat tgtactatgg aattattcta
    23821 aaacgctccc aaaaaggagg tgcatataaa attgaaacct actatttatg acattgcgaa
    23881 aaaagctaat gtctcaaaat caactgtctc cagagtatta aataaccagc caaatatctc
    23941 tgataaagca aaagcaagtg tacttcaagc aatcaaagaa ttgaattatg aaccgagcaa
    24001 aattgcaaga ggactgtcag gaaatggttt tgacgcgatt ctagcattat ctcatcgtac
    24061 ttctcatacg acctatggca atcctttttt ttcagatgtt attcaatcca tttctgccgt
    24121 ttctgaacaa aataacttcg acttgattct acaaacggca aaaacaccag aagaagagct
    24181 agaaaaatgc gcgaaaaaga tccaagaaaa aatgatccga ggaattatta ttttaagctc
    24241 tacgatggat gaaacacttc tttttgaatt ggatcaatac aatattccta ttgtggtaat
    24301 cggaaaagtc ggaaacgact acacgaacat ctactcagtg gacacggata atttccagga
    24361 tagttacgat ttggtggaaa cactgatcaa gaatggacac cgtcatatcg gctgtctcca
    24421 tgctccgaca gaagtttatg tatccaatga tcgtctcaat ggttaccgtc aatgcttatt
    24481 tgatcaccac ctagatatcc gtaatgaatg gattatagac ggcggctttc gcttagaaga
    24541 tagcatggca gcaatgaatg atttaatgaa gctacgagaa agaccaactg cgattttttc
    24601 taccgatgca ttaaaatcat tgagtattta caaagtggca gaacaatctg gaaagcagat
    24661 acctgatgat ctttcgatcg caggattcag tgatccctta ttgtcctccc tattttcgcc
    24721 acagttgact cggatagaca ttccgacaaa tcagttagga atcaaagcca cagaactttt
    24781 attccaacta atccaccacc aataccctga agaaaaaaga atcattcttt caaccgagat
    24841 tactctcaca gattcgatcc aacaacaaga aatgtagaac agaattataa aagaacagaa
    24901 acactgagga taagtcagtt actctgttct tttttcaatc actcatccct cgttaccccc
    24961 tcccggtatt ttagcgaatt tttcttattt tccatctgtt ttagatagct gtggataact
    25021 ataatgtttg tgttaaacta ttttttccac agggaagaaa aaactatttt taaccacgtt
    25081 gtgtataagt ttttatagtt cgttttttta aacacattta cccttatttc aatccatgtt
    25141 atttggggat acttatctct aaaacataaa tagaataaca gacatgtcta ctggttttgt
    25201 tttattcgag gaattattca ataatagcta gcaaaatatt aaaaataacc gctctatctt
    25261 cccatcaatt tattttgcat tcaaaaaatg atctatcttt acttttgttg attgacttct
    25321 cttggaagat gatcgttccg ataccttaat tggatacgaa ccgatgttgt taagatcctt
    25381 tcccactttt cttctagtta gacaaattat tttagacaaa ccaaaaaaga ggttgtgaca
    25441 ataatttgtc acaacctctt ttttaaaata tctattttgt ttgattgttc attgcgtctt
    25501 ctgcagccat ttgcgcttca atatctgaaa tactgtagac attttcgctt gctacaccga
    25561 cttcttttgg ttccatgtca cgataacgtt gcataccagt accagctggg atgatcttac
    25621 ctataataac attttctttc aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat
    25681 ccgtcaagac acgagttgtt tcttggaatg atgcagcaga taagaaacta tttgtttcta
    25741 atgatgcttt tgtgatacct agtaagacag gtcgagaagt tgcaggaacg cctcctgaga
    25801 ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc taataatgta cctggtaaga
    25861 tatctgtatc acctggatcc attacacgaa ctttacgcag catttgacga accattactt
    25921 cgatatgctt gtcgccgatt tctacccctt gcatacggta aacacgttgt acttcacgaa
    25981 gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa caattgtttt ggatcgattg
    26041 atccttctgt caatggtgca ccacggtgga tcatatcgcc ttctgctact ttcatacgtg
    26101 ctgtataagg aacagtatac gtacgtgtat ctgtttttcc tttgattgtt acttctttgg
    26161 tacgtgttgc aggatcttct gagatatcga tgacatcccc tgttacttct gtgatcactg
    26221 cttgcccttt cggattacgt gcttcaaaga tttcttggac acgaggaaga ccttgagtga
    26281 tatcgtctcc ggcaacccca cctgtatgga atgtacgcat cgtcaactga gtaccaggtt
    26341 caccgattga ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg tcagagccag
    26401 ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg ttttgtatta catgtgaaga
    26461 cagaacggat tgtcactgtt tcgacacctg catcaacgat ttgttttgcg atgtcttcgg
    26521 aaatcaattg gtttcctgca atgatcaatt catttgtttc aggatgacgt acttctttac
    26581 gggtgtaacg tcccactaaa cgatcttcta atgattcgat gatttcattt ccctcgcgga
    26641 tggcttggat ttcaagacct cggtcagtac cgcaatcttc ttcacggatg attacatctt
    26701 gggccacgtc aaccagacga cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat
    26761 cggtcatccc tttacgagcc ccgtgagttg agataaacat ttccaagacg gatagtcctt
    26821 cacggaagtt cgaaatgatt ggcaattcca tgattcgacc attaggagcg gccatcaaac
    26881 cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc acgagctcca gaatcagaca
    26941 tcatgaagat tggatttttc gcttccaaac cttccatcag tttttgttgg atgctgtctt
    27001 ttgcagcatt ccaaacagca atgacacgtt cataacgttc gtcatctgtg atcagaccac
    27061 gacggaattg ttttgtaata gtttcaactt gtttatgggc attttcaatg atttcttgtt
    27121 tttcattcag tacactgatg tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt
    27181 tgtaccctaa gtctttcata cggtcaagca tcttagaagt ttctgttact ttgaatcgtt
    27241 tgaagacttc tgcgatgatg tttcctaagt ttttcttctt gaacggtaat actaattctt
    27301 gctctttgat atgtgcagga atatcagtac cagcttcaac aaagtatttg tccggtgttt
    27361 ggacggtcaa gttgaagtct gttggttcat tcagataagg gaattcagga ggcatgatct
    27421 cgttgaagat gatcttacca actgttgtga tcatcgtacg ttctttttgc cattcagtaa
    27481 atggtttttc acctaatgag ttagggttca caccgatacg tgaatgtaaa tgaacatatc
    27541 cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa gaccattcct tcgccttcgc
    27601 gtccttcttc ttccattgtc aagtagtagt tacctagaac catatcttga gaaggagtaa
    27661 caactggttt accatcttta gggttcaaga tgttttgagc agctaacatc aacatacgtg
    27721 cttctgcttg tgcttcttca ttcaatggaa cgtggacagc catttggtct ccatcgaaat
    27781 cggcattata agcttcacat acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa
    27841 ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg tgcccggttc agcaatactg
    27901 ggtgttcctt gattacgtct tctaaaacat cccatacttc gtcttcttga cgttcgattt
    27961 tacgtttcgc atttttgatg tttgaagcta gttcgcgttg aacaagctca cgcatcacga
    28021 atggtttgaa taattcaatg gccatttctt ttggaagtcc acattggtac atcttcaaga
    28081 aaggtccaac aacgataacg gaacgacctg agtagtcgac acgtttacct agcaagtttt
    28141 gacggaaacg accttgtttc cctttcaaca tatgagaaag agatttcaat gggcggttac
    28201 ctggtccagt aactggacgt ccgcgacgac cgttatcgat caatgcatct actgcttctt
    28261 gcagcatacg tttttcattt tgaacgatga ttccaggtgc attcaagtct aacaaacgtt
    28321 tcaaacggtt attacggttg attacacgac ggtaaagatc attcaagtca cttgttgcaa
    28381 aacgtccacc ttctaattga accattgggc gtaaatctgg tggaataaca gggataacgt
    28441 ccataaccat ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa atatccaaac
    28501 ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt caattcttct ttcaattcgg
    28561 ctgcttctgc atctagatca acattatcaa gtaattgttt aatcgcttct gcacccatag
    28621 cagcttggaa ttcttgtcca tattgttcac gtttttcgcg atattcgcgt tctgtcaaca
    28681 attgtttttt ctctaaattc gtatttcctg gttcgatgac tacatatgaa gcaaaataaa
    28741 tgatttcttc caaagcgcgt gggctcatgt ctaatacaag acccatacgt gaaggaatcc
    28801 ctttaaagta ccagatatgt gaaacaggag ctgctaattc aatatgtccc atgcgttcac
    28861 ggcgaacttt cgaacgagta acttccactc cgcatcgatc acaaacgatt cctttataac
    28921 ggatacgttt gtattttccg caggcacatt cccagtcttt tgtaggacca aaaatccgct
    28981 cacagaacaa cccttcgcgt tcaggtttta aggtacgata gttaatcgtc tcaggttttt
    29041 ttacttcacc gtaagaccag cttctgattt tttcgggaga agccaaacct atttgcatac
    29101 tttcgaattt atttacatcg atcaaaaggg gttccctcca tttcatttaa tggcaaaccg
    29161 caagacggct gatcttcgac cgccttgcag ctcatagacc ggattagtct aaattatctt
    29221 ctgcggtttc aaagttttgg atgacatcgt ctttttcatc ttccacttgt tcagcagctt
    29281 tcttttctaa ttctttggca gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga
    29341 tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct tcgatatcca
    29401 agacacgcat gtccagacct agtgattgca attctttcac caatacgcgg aatgattccg
    29461 gtacacctgg ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc
    29521 cgactacgtc atctgatttg tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag
    29581 cttccagtgc ccatacttcc atttcaccaa aacgttgtcc accaaattga gctttacctc
    29641 ccaatggttg ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc aatttgtcat
    29701 cgaccatgtg agcaagtttg atcatgtaca tcacaccgac agacacacgt ccatcaaatg
    29761 gttcacctgt acgtccgtcg tacagaacag ttttcgcatc gctggccata ccggcttcac
    29821 gaactgtttc ccatacgtct tcatcacttg caccatcaaa tactggcgtt gctacgtgga
    29881 tacctaactg acgtgcagcc atacccaagt gtaattccaa tacttgcccg atgttcatac
    29941 gtgaaggcac ccctaaagga ttcagcatga tatcgattgg tgttccatca ggtaggaatg
    30001 gcatatcttc ttccggcata atacgggata caaccccttt atttccgtga cgaccggcca
    30061 ttttatcccc ttcatggatt ttacgttttt gaacgatata aacacgaact aacatgttta
    30121 cgcctggtga caattcatct ccagcttcac gagtaaagat tttcacatcg tgaacgatac
    30181 cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa
    30241 agatcgcatg caatagacgt tcttctgcag ataattctgt tacccctttt ggcgtgactt
    30301 tccctacaag caaatcgcca tcttggactt ctgcaccaat acggatgata cccatttcgt
    30361 ctaaatcttt taatgcgtct tccccgacat tagggatttc gcgtgtgatt tcttcaggtc
    30421 ctaattttgt atcgcgggct tctgattcat attcttcaat atggatagaa gtgtatacgt
    30481 catcttttac caaacgacgg ctcatgatga tcgcatcttc atagttgtac ccttcccaag
    30541 tcatgaaacc aactaaaacg ttttggccta gagccatttc gccttgttcc atagatggtc
    30601 cgtctgccag agtatcccct ttttcgactt tctcacccaa atgaacgatt ggacgttggt
    30661 tgtagcttgt tccggagttt gaacgacgga attttgttac atcgtattta tctaatgcac
    30721 cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta ttcaacgaca ccgtcatgtt
    30781 tacataacaa agcagctcct gagtcatgcg ctgatttgta ttccatacca gtaccaaccc
    30841 atggtgcttg aggattgatc aacggcactg cttgacgctg catgttggca cccatcaagg
    30901 cacggttgga gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg gcaactactt
    30961 gtttaggtga tacgtccatg tagtctactt tatcaataga tacttctaag ttttcacttt
    31021 gagcacgagc cataacaacg tcttgagcaa atgttccatc ttcattcaat gggctgtttg
    31081 cttgggctac gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa
    31141 cacgacctgt ttcacggtct acacgacgat aaggtgtttc gataaatccg aatttattta
    31201 ctttcgcata actggacaaa ctattgatca acccgatgtt tgggccttca ggagtttcga
    31261 tcggacacat acggccatag tgggagtagt gaacgtcacg aacttcataa ccggcgcggt
    31321 cacgagtcaa tccgccaggt cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa
    31381 gagggtttgt ttgatccatg aactgtgaca actgagaaga accaaagaat tctttgatac
    31441 ttgccacaac tggacggata ttgatcaatt gttgaggtgt caatgtttca gtatcttgga
    31501 tcgacattct ttcacgaacc acacgttcca tacgtgctag cccgatacgg aattggtttt
    31561 gtaaaagttc accgactgaa cggatacgac gatttcctaa gtggtcgata tcatcgacat
    31621 tgccgatacc ttccatcaag ttcaagaaat agctcattga agcaatgatg tcggctggac
    31681 gaaccgtttt tacagctgct tctggatagc cgtttccgat cacgtttact tcacgttcag
    31741 ggtctcttgg tgaaaatact ttgatgactt ggacagtcat tggatctgtg accacaccgt
    31801 cttctgatgg atagtaagtc actgaattta ggccattatc aatgaaagga gctagtgttt
    31861 ccatgacttg gtgagtcaga actgttcctt tttcaacgat gatctcgcct gtttctggat
    31921 caactaatgt ttcagctaat gtcagattca atagacgtgt tttcaaatct aattttttgt
    31981 ttactttgta acgaccaacg tttgctaagt cgtaacgttt tggatcgaag aaacgagcat
    32041 tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa
    32101 cgtctttcaa accttcttct gttcttgagt cgcttgcgtt tttatgcaag tctttttcga
    32161 ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa
    32221 atcctaacgc acgaactaat acggttaaag gaatcttacg tgtacggtcg atacgtacat
    32281 aagagatgtc ttttgcatca gtttccattt ctagccaagc tccacggtta gggataacag
    32341 tagaaccgaa tccttctttt ccgtttttgt cgacttttcc atggaaatag acacctggtg
    32401 aacggactaa ctgagaaacg attactcgct ctgccccgtt gatgatgaat gttccttgtt
    32461 ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc
    32521 ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta gtttgcatca tgcgcacgag
    32581 cttctgctac tgtatacttt ggttctttca attcataatc tacaaattcc aaagatagat
    32641 tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta
    32701 agaaccattg gtaagagtct gtttgaattt cgatcaaatt tggtaattcc aacacttcac
    32761 tgattcttgc gaaacttcta cgttcgcgat gttttccgta ttttactacg tgtccagcca
    32821 agctcttcac ccctctaaaa tgtcattcaa aaagactacc cgatcaattg ttacaataat
    32881 atttcaaata ttatttttag gtaacaacag acattttttg cggcttttag gcaaaaaaaa
    32941 accaaaaata gaacattctc ttgaaacctt acttcgtgag cttctacttt tgtttcctta
    33001 tattaaagcc gaaacggaca atatttcgtt tctgcctttt cttcatcaga tagtttttgc
    33061 aacttcatat actacactta ttttctataa aagtcaacac ttcctggcgt ttcttctgct
    33121 ttttcgcaac attttcttaa tgttttcaat tttatgtttt caattatctg acaattctta
    33181 attgacagac ctttttatct cggttatact ttagaaaatt cattttgtgg aggaacttct
    33241 atgcgaacca acatcaacca aatcgcacca acacaactga ataactttta ctttagctac
    33301 tttagatagg tttgtattca tcatgatgga tacaaacgtt aaacgacagt ttgtatctat
    33361 gatggctaaa gaactttgta ttgcagcaaa aagccacgta gacaaaatgc taagtggctg
    33421 agcagtgaaa atacaaagta aaagtatcaa gcattctact ttttcaattt attttccgac
    33481 ttttcaatcc atttggacat tttgtctgaa tggatttttt gtttcccaaa attaaggagg
    33541 aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta ctagctttag
    33601 gaggttgcgc acaaaaagac aattccgcag aggcatcaga tggacaaaaa acaattggcg
    33661 tattgcaact tgtacaacat aactcgttag atgctgccta taaaggattc aaagaaggat
    33721 tagcagaaga agggtataaa gaaggcgata atctaacaat cgattatcaa aatgcccaaa
    33781 acaatcaaga caacctgaaa agtatgagtg agaaactcgt caaagaagat cctgatttgc
    33841 tacttggaat cgccactcca gcagcacaat ctttattaaa tgaaacaacc actatcccga
    33901 tgactgtcac agcggtcaca gacttggaac aagcccattt agtcacatcc aatgaaaaac
    33961 ctggcggcaa tgtgactggc acgacagata tggtgccaat cgaaaaacaa atcggcttac
    34021 tgttaaatat cgtgccagac gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa
    34081 actctaagat ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca
    34141 aagtactgac agcaaattcc acaaatgatg tccaacaagt gacgaaaagt ctagctaaag
    34201 atgtagatgg aatctatatt cctactgata acacgttcgc aagtgcggca agtgttgtcg
    34261 gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat caagtcaaag
    34321 acggcggtct agcaacagta ggaatcgact acgaaagctt gggcaaacaa acagggatca
    34381 tggcagcaaa aatcttagac ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg
    34441 atttggaact gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat ccagacagca
    34501 tcaaagaagc tgaataaaac acaggaggat tctcatgaca aatttgctct ctcttttctt
    34561 atcatctgca tcgcaagggg tcttatgggc cctacttgcg attggtgtat acgtcacttt
    34621 ccgtatcttg gacattgccg acttgactgc tgaaggaagc tttcctttag gtgcagggat
    34681 cgcggcagta agtattacaa atggctatca tccattgaca gcttgtcttc taggcttttt
    34741 agggggtgcg gcagcagggc ttgtttccgg actgcttcac acaaaattga aaattcccgc
    34801 ccttcttgca gggatcatca ccatgactgg tttatattct gtcacatccc gtgtgatggg
    34861 tgcgccgaac atctctttat tgggtcagcc aacgatcttt cattgggtcg agtcattcgg
    34921 cataacaaaa gacaacgctg tactgatcgt cggtctgctt tttgctttga tcgttgtatg
    34981 tttacttgtt ttgttcttac gtacagaaac aggacttgct attcgtgcaa ctggcgataa
    35041 tctggcaatg agcgaagcaa acgggatcaa cacagataat atgaagatca tcggttatat
    35101 gatttctaat ggatgcattg ctctttctgg ctccttactt gcgcaaaaca acggctttgc
    35161 tgatttaaat tctggtattg gaacgatcgt aattggactt gcttctatta tcatcgcaga
    35221 agttttgtta cgtaaccagc cactatttct ccgtctcttg actgtcgttt taggtgcggt
    35281 catttatcgt tttattttag cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa
    35341 acttgcttct gctttcgtgc tagtgatctg tctttctttc ccgcagatcc agcaaaaatt
    35401 ccatttacct caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt aacattggaa
    35461 aatctccatc atacctttga aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt
    35521 aatctttcct tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa
    35581 tctactttgc tgaacagcat cgccggtaca ttttctgtcg atcagggtca aattttattg
    35641 aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta
    35701 ttccaagatc ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt
    35761 gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca
    35821 tttttcaaag accagctggt tcggctaaat ttggggttgg aaaatcgttt atcctcagaa
    35881 atcggtttgc tgtctggagg acaacgtcaa gctattacgt tattgatggc cacattacag
    35941 cgtccagaac tgatcttatt agatgaacac actgcagcgc ttgaccctgc tacttccaaa
    36001 acggtgatgg aattgacaga aaagctgatc actgagcaga atctcactgc ttttatggtc
    36061 actcatgaca tggaagacgc gatccgctat gggagtcgac tgatcatgct gcatcaagga
    36121 aggatcgtcg tagatatcca gcaagaggaa aaacagcagc tcacagtgcc ccaattatta
    36181 gcgttgttca aacaaaacag cggcacggca ttaaaagacg atcaagtact tttatcttca
    36241 taatcagaaa gaggatggga caaa
//
LOCUS       DACCCR010000024        35883 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCCR010000024 DACCCR010000000
VERSION     DACCCR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.024"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1152
                     /locus_tag="IVK93_001153"
     CDS             <1..1152
                     /locus_tag="IVK93_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386456.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IVK93_001154"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IVK93_001154"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP6386457.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IVK93_001155"
     CDS             2728..3003
                     /locus_tag="IVK93_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP6386458.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IVK93_001156"
     CDS             3009..3776
                     /locus_tag="IVK93_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP6386459.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IVK93_001157"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IVK93_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP6386460.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IVK93_001158"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IVK93_001158"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP6386461.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IVK93_001159"
     CDS             7920..8222
                     /locus_tag="IVK93_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP6386462.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IVK93_001160"
     CDS             8225..9388
                     /locus_tag="IVK93_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6386463.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IVK93_001161"
     CDS             9414..12842
                     /locus_tag="IVK93_001161"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP6386464.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKTIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IVK93_001162"
     CDS             12863..13987
                     /locus_tag="IVK93_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386465.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14415
                     /locus_tag="IVK93_001163"
                     /pseudo
     CDS             13977..14415
                     /locus_tag="IVK93_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
     gene            14399..14737
                     /locus_tag="IVK93_001164"
     CDS             14399..14737
                     /locus_tag="IVK93_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP6386466.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14856..15416
                     /gene="rsmD"
                     /locus_tag="IVK93_001165"
     CDS             14856..15416
                     /gene="rsmD"
                     /locus_tag="IVK93_001165"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP6386467.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15413..15904
                     /gene="coaD"
                     /locus_tag="IVK93_001166"
     CDS             15413..15904
                     /gene="coaD"
                     /locus_tag="IVK93_001166"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP6386468.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15894..16937
                     /locus_tag="IVK93_001167"
     CDS             15894..16937
                     /locus_tag="IVK93_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6386469.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17096..17824
                     /locus_tag="IVK93_001168"
     CDS             17096..17824
                     /locus_tag="IVK93_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP6386470.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17940..18185
                     /locus_tag="IVK93_001169"
     CDS             17940..18185
                     /locus_tag="IVK93_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6386471.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18182..18400
                     /locus_tag="IVK93_001170"
     CDS             18182..18400
                     /locus_tag="IVK93_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP6386472.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18536..19075
                     /locus_tag="IVK93_001171"
     CDS             18536..19075
                     /locus_tag="IVK93_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP6386473.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19068..21341
                     /locus_tag="IVK93_001172"
     CDS             19068..21341
                     /locus_tag="IVK93_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP6386474.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21379..22425
                     /gene="holA"
                     /locus_tag="IVK93_001173"
     CDS             21379..22425
                     /gene="holA"
                     /locus_tag="IVK93_001173"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP6386475.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22514..22768)
                     /locus_tag="IVK93_001174"
     CDS             complement(22514..22768)
                     /locus_tag="IVK93_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP6386476.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23168..23437)
                     /locus_tag="IVK93_001175"
     CDS             complement(23168..23437)
                     /locus_tag="IVK93_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386477.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23907..24362)
                     /locus_tag="IVK93_001176"
     CDS             complement(23907..24362)
                     /locus_tag="IVK93_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP6386478.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24500..26428)
                     /locus_tag="IVK93_001177"
     CDS             complement(24500..26428)
                     /locus_tag="IVK93_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP6386479.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26625..27458)
                     /locus_tag="IVK93_001178"
     CDS             complement(26625..27458)
                     /locus_tag="IVK93_001178"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP6386480.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27650..28354)
                     /locus_tag="IVK93_001179"
     CDS             complement(27650..28354)
                     /locus_tag="IVK93_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6386481.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28377..29003)
                     /locus_tag="IVK93_001180"
     CDS             complement(28377..29003)
                     /locus_tag="IVK93_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6386482.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29329..31704
                     /locus_tag="IVK93_001181"
     CDS             29329..31704
                     /locus_tag="IVK93_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP6386483.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31728..32219)
                     /locus_tag="IVK93_001182"
     CDS             complement(31728..32219)
                     /locus_tag="IVK93_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP6386484.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32406..33326
                     /locus_tag="IVK93_001183"
     CDS             32406..33326
                     /locus_tag="IVK93_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6386485.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33480..33824
                     /locus_tag="IVK93_001184"
     CDS             33480..33824
                     /locus_tag="IVK93_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP6386486.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34014..34514)
                     /locus_tag="IVK93_001185"
     CDS             complement(34014..34514)
                     /locus_tag="IVK93_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6386487.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35042..35125
                     /locus_tag="IVK93_001186"
     tRNA            35042..35125
                     /locus_tag="IVK93_001186"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35076..35078,aa:Leu,seq:cag)
     gene            complement(35449..>35883)
                     /locus_tag="IVK93_001187"
     CDS             complement(35449..>35883)
                     /locus_tag="IVK93_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6386488.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11871 a         6261 c         7404 g        10347 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaaac gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aaagtttgcc agttgaaaga agcgttcttg tctaagaaag aagcatttga aaatgtagcg
    14161 gcatatggtg agtatgcacc tgattatcgt gaaaaaaaac gagctgttcg aaaagcaaaa
    14221 agacagcttg atatggatga gcatgttgca gctttccgat atagtgaaac acagctgcaa
    14281 agtctgttgg atcaaattgt ccaagtactt gccgaagaag tttctaatga aatcaaagta
    14341 gatgcaggta gcccgttttt tgaaacgaaa aaacactttg gatgcggagg taattgtcat
    14401 gcaattagac aatagtgaat ttgaaataac aaaacgacgc gggctgatcg tatgggtcta
    14461 cagcttaaaa caattaaaga atctgaaaaa gttcggcttg ttgcattatg tctcaagaag
    14521 aatgaaatat gtcgtgcttt acttaaatga agagacattg gaagaaacag aagaacgaat
    14581 ccgtaaatta cattttgttc gaaacgtaga acgttcttac cgaccggata tcgaaatgaa
    14641 ttttgccgaa aaaataggaa acaaaacagt attaaaagac gaaggtttcg aaattgaaga
    14701 gctaaataca gaaatccgat tagctgaaca tctttaaaaa tctcttatga gcagttttta
    14761 aataaaaaat aacttggata acgatcgatt tttgatcatt gtccaagtat ttttttggta
    14821 tactagtacc agtaccattt ctaaggagtg agtagatgcg gataatatca ggaaattatg
    14881 gcgggcgccg tttaaaaagt ttagcagggg cgaacaccag accaactaca gataaagtaa
    14941 aagaatccat attcaatatg attggaccat attttgatgg agagacagta ttggatctgt
    15001 ttgcaggaag cggtggttta gctatcgaag cagtatctag aggctgcagt catgcggtat
    15061 gcgtagacaa aaattaccaa gcgctaaaaa tcatcaaaga aaacatcgaa attacgaaag
    15121 aaccagagaa atttaccaca ataaaacttg atgcggacaa agcaatcgaa cggctagcag
    15181 cggagaaaca acaattcgac tacttgtttc ttgatccgcc atatgcaaaa caaaaaataa
    15241 tcgatcaaat ccataaaatg gaagaactgg gacttttttc taaaaccgca gttatcgtat
    15301 gcgaaacgga taaaacagtc gaattgccag aaactgtggc tggattccat caaataagaa
    15361 aacagattta tggcataacg gccgtaacta tctatagaaa agagggaaat caatgaaacg
    15421 tgcattattc ccaggaagtt ttgatccttt tacaaaaggt cacttagata cggtggaacg
    15481 tgcggctaaa ttattcgatg aggtagtaat tggtgttttt atcaatactt caaagaaaag
    15541 tttgtttccc ccagaagaaa gaatgacatt gatcacaaaa gctgtttctc atcttcctaa
    15601 tgtaaaagtc atgcatcaag aaaatcaatt gacggtagaa acagcaaaag agattggtgc
    15661 agatgcgttg atcagaggca tacgaagtat caaagacttt gaatatgaac gagagattgc
    15721 tcagatgaat catcatctat atgatgaatt ggaaactgtc ttcttattag caaaaccaga
    15781 atatagtcac gtgagttcca gtattttaaa agaagtactg cactttggcg gagatgtgag
    15841 cagctactta ccgccagtga tcaacgaagc tttagctgga aagcgtgaat caaatgaaag
    15901 ctaaacaatg gttgaaaaca gtactgatta tcatatttgg aatgttgatc attggatttg
    15961 tagtagttcc cttgccgtat tatgtagaat caccgggagc cacgatcgat ctgaaagaac
    16021 taattacggt caatgataag gaagataaag gttctgggtc tttctctctt acctctgttg
    16081 gtatcaggca agctacaggt ttcttagctt taaaagctaa aatgacccct tttgaagaat
    16141 tgatcagtga agaagaattg actggtgggg cgacaaatga agaatataat caaatgcaac
    16201 agtattatat ggattcttcc caaaatgcag caatcgaaca agcgttaaaa cttgcagaca
    16261 agccttataa aatggaattc aaaggcgtat atgtcatgag tgtagagaaa acatcgaatt
    16321 tctttggcaa gctttcagtt ggagatacag taacaaaagt agacgggaaa agctttcaaa
    16381 gtacagaagc atttatggat tatgtaaaaa gccaaaaagt tggacaaaca atcgaaatca
    16441 gttatttgca taatggtgaa gaaaaaacag cttctggtga tctgatcgag ttaccaacag
    16501 ataaaaaggc aggaataggt atcacgctta ctgaccatac cgagatcgaa tcagatacag
    16561 atgttcgtat taattctggc tctatcggtg ggccatcagc gggattgatg ttcactttag
    16621 aaatctatga acaagtcact ggtaaaaact tgcgacatgg taagcaaatt gcaggaactg
    16681 gaacgatcaa cagtgaagga gaagttggcc gaatcggagg aatcgataaa aaagtggcaa
    16741 gtgcggataa agctggcgta gagattttct ttgcaccaga tgatacgatt tcagacgatg
    16801 ttaagaaaga atatcctgaa ataaaaacca actacgaaga agcaaaagct gccgctaaaa
    16861 aattgaattc ctcaatgaaa atcgtaccag taaaaacagt acaagaagcg cttgactatc
    16921 tagagaaaat gaaatgaaac taaataatta aagaggggtt gtgacacgag ttctgtcaca
    16981 acccctcttt ttcgtcaaaa atcttcacga taaaaataaa aacaaaagaa aaaattgacg
    17041 taattagatg ttgaagggac gattttgcca gagctcactg tttcttcaat tttaaatgaa
    17101 aggagtaatg aatgattttt actggtcatt ctttgaaaaa agaacaatga tagaaaaaat
    17161 cagcaagaaa atactagctt cacccaaatt gatcatttgg atgtctatga tagttcttat
    17221 cttttgtatc gcaagtggtt ttttattatc aaatttgcta ttttatagag aaacagaaga
    17281 gactgctgca aaagaaatac tttcttcgtt tagttccagt ggagaagatc catcagctga
    17341 aggaaacgat gtagaagagg atgctcgtcc aaaggtcatg tacaccgata tcaaaggaag
    17401 tgtgaaggag cctggtatct attccttttc ttcagaagaa cgggtctatg atgtactaaa
    17461 gcgtgcaggt ggattattag aagaagcgga tagcgatcga atcaactttt ctgcgaagat
    17521 agaagatcag caagtcctat atatccctgc tgtaggtgaa gagctgccag aacatcttga
    17581 tcagttggca tcctcggaag gaaaacagcc tacagcagat tccgagcctc ccaaaataaa
    17641 tatcaataca gccactccat cagaacttca gcagattcct gggatcggat cagtcaaagc
    17701 gcaagaaatc attcgattta gagaagaaaa cggatccttc cagaaagtgg aagacctgca
    17761 ggagatttct ggtatcggtg aaaagacagt tgaaaaacta aaaaacttcg ttacaattaa
    17821 atagaatgat tcaaaatcga ctggcacgag caataaatgg acgctaatgg atactaagaa
    17881 taatccaatt ctaagagctt ggcaggacga ggaggtagta tttttggata ggaaatagct
    17941 tgattgttcg caaagaaaca ttaaaaaaac caatgctgaa tgtctatctc caaaataaaa
    18001 tatctgggat tcatattatg aatacggctg tcagcggcaa taacagtcaa gctctacgag
    18061 aacgctttgc caaagatgta cttagctata cagcagataa agtatttatt ctcattggga
    18121 cgaatgatct agcagagcat aaacaacttt ctaaagaaac gtaccaaaaa atctgcagtg
    18181 gatgatagtt cagctgaaaa aagactatag ttctgatcat gctttcttta tcacgccacc
    18241 agcagtcgat gagacaaaac aaaaatatcg gaataacaag ttgatcaaag aatatatctc
    18301 tgttctcaca gaagtagcaa aacgccatga ttgtgaagta tcgattttta tacggaattt
    18361 cttgaacaaa tggattcgaa gaatttttat aatgggctaa tggatgacgg acttcatttc
    18421 ggcagtgaag ggtaggatct tttgtcagac aggatgattc gttatattta aagaatattt
    18481 tgtttaagca atgagtactt tttaatcaac ttgaaaaaac ggaggaatat aaaacatgtt
    18541 agaaaaatct tcaaaagcat gcgatacgaa taaagaaaaa gttactgata agtcacataa
    18601 tgaacgcatt ccttgggacc agtactttat ggctcaggca gtactgttat ctttacgtag
    18661 tacgtgtaca cgtttagaag taggagcgac actcgttcga gaaaaaagga tcattgctgg
    18721 cggctataat ggagcagtca gtggagatgt ccactgcata gatgaagggt gttacgtcgt
    18781 ggatggacac tgcttgcgaa cgatccatgc agagatgaat gcactcctac agtgtgcaaa
    18841 acttggtatc ccgacggaag gttcagaaat ctatgtcaca cattttccat gtttggcctg
    18901 tacaaaggct ttgttgcaag ctggtgtgaa agggatcaat tatctccatg actatcgaaa
    18961 tgatccttat gcccaagcgt tgatcgaaca attaggtgta tctgtacacc aagttgcttt
    19021 agactcacag catttttcaa aacttcaaaa tgaattaaca ggaaaacatg tctagcgttt
    19081 tagaaaggca tgttcgaaat cggttgattt ttccggcaat tacgtcgatg attatctgtt
    19141 tgttgtatta cactgacaat tggggttatc gtttgcttct tgccggaatt tttttatggt
    19201 tttcgctaaa agaaggacgt tttatcgcgt acttgtgtat tttgtgtagc ataacggtat
    19261 ccatttctta taggctacaa gagtattcat ttttagagga aaaaacaaca tctgcgcaaa
    19321 ttactgtatt tacagatacg attcgtgcaa atggtaattt tttgacaatg gaaggaaggt
    19381 tatcagagaa taaacaaaag ctccagcttt cttatcaagt aaacacagaa gcagaattga
    19441 aaacatggct taacttccga ggaaagggaa tagaattgac tgctgaaggc gagttcatct
    19501 ctccagcaaa aaagcggaat ctttataatt ttgaccaaga agcttacttc aggaaccata
    19561 gaatttctgg acggttccaa attcaaaaga ttgaacagat caccatgcaa aatcactggc
    19621 gaaactggct tcagagaaaa agaggatctt ttatctcctt tgtccaatct gaatttccgc
    19681 aaaaaacaag tgtatatatt aattctttat tatggggcta taaagatgca ggattcagag
    19741 aaccagaaga aatttttagg gagagtggtt tgctgcatct gttcagtcta tcgggtctac
    19801 acatccaatt ttatttaggg tggctgtatt atttatttcg acgtatcgga tggccactac
    19861 acgtcagtat tattccgttg agtctactaa ctatctgtta tgtttctatt gcaggaagtt
    19921 ctatttctgt tttgcgtgct agtttgctgt ttttattgcg attgctagtc aaattgctga
    19981 ataagcatta ttccaccatg gacttatttg cagttgtctt ttggcttctt ttactatttg
    20041 atcctcgatt tctttttcat gcgggtggac agcttagttt tttcatgagt tttttgttta
    20101 tcctgatttc ttttgatgga gattggttga aaaaaacggc ctctcacgtc ctgtttacta
    20161 tgatcacgat gccgttgatt gtatggcatt tttatgagtg gcctatttta ggtagtcttt
    20221 ttacattact gattgcaccg gtattcaagt ttctttttct tcctggtgtg ttatttctca
    20281 cactcttcaa tcgctggctg ccggaaactt tacttttgct gatagaagag ggattaatct
    20341 tatttgagcg aataatcgat ttttcttcag ggtcgtctct cattatagga cgtttgcccc
    20401 ttgtgctatc tgggatgtat attttcggat tactgatttg tatggatagg ttgaaagaac
    20461 aacctgtgaa acacttgttt tatgttggtc ttttttcttt tatttttttg actttgccat
    20521 ttttacgctt ttcttcaacg atcgctttta tcgatgtcgg tcaaggagac agtatctttt
    20581 tgcaaagtcc ttttcgaaaa gaaacgattt tgatcgatac tggaggtcag ttacactttg
    20641 aaagagaaaa gtgggcgcaa ggtatgatac gtcctcctgc tgaaagaaat atcgttccat
    20701 atctaaaagg tcaggggata agtgtaatcg ataaactcat cttgacacac gacgatacgg
    20761 atcatgtggg agaattaccc acgttatctc agcattttac tatcaaaaaa atttatatcg
    20821 gttggggcgc tggacgaaat gagccattac aaaggcattt atctgaagct caaaaaaatg
    20881 gtactgagat cattgaaatc aaacatgggg acaggatcag cggttattat gatctttatg
    20941 tattgactcc ttttgaaaag ggagagggaa gaaatgaaga ttcgattggt ctatggatgg
    21001 aatataacaa taggcgcttt ttatttttag gagatttgaa tcaagcaatg gaaaaacaat
    21061 tgcttttgat ttatcctaat ctgaaagccg atgtagtcaa attaggtcat cacggtagcc
    21121 gtacgtcaag taacacagat tttctttccc atattgaagc aaaacatggg attatatcct
    21181 gcggcgtaaa taatcgttat ggtcacccgc acagcgaagt attggagaca ctagaagaaa
    21241 atcagataaa aacatggcga acggatcaac aaggaatgat cacctatcgc tggcatccag
    21301 tttttcatcc tcatgggcta gtggaaacaa tgatagacta gtaattctca tctgctcatg
    21361 gtaggataat ttttgaaagt ggtgagtaat ttgagtatac aagcagagtt acaaaaaata
    21421 cgtaaagaaa aacttgcatc ttgttacttg attttaggaa cagaaaagtt tctccaagat
    21481 caagttcgga cagaaattgt caaaaaaatc caattagagg gcgaagatga cttaaatttt
    21541 ttaagttttg acatggaaaa tgcaagttta gatgaggtag tagctgaagc agagacgttg
    21601 ccgtttttcg gagatcaacg gttagtattc gtagagaatc cttatttttt aacaggtgaa
    21661 aaagtaaaca atggaatcga acaaaatact gatttattga cggcttattt gaaagaacca
    21721 ttagaatcga ctgtattagt attctttgcg ccatatgaaa agttggatga acggaaaaaa
    21781 gtaacgaaac aattgaaaaa aacagcaatt atcatcaatg tccaacagtt gaatgaaaaa
    21841 gaagtacgtc aatatctgat gaatacctta gagaattcct caatcaaaat ggaacgaagt
    21901 gcaattgatt tattcttgag gttgaccgac ttagatttat ccaaaatgat gagagagtta
    21961 cagaaattgg tcctttatgg acaaaatcaa cagcaaatca cggtaagaga agtagaagaa
    22021 ttagttccta aaacgttaga acataatatt tttgacatga cccaatatat tttaaaaggg
    22081 aagacggaac aagctttgcg tttatacgaa gatttagtta tgcaagggga agaaacaatc
    22141 aaaattaatg cgattttgct ttctcaatta cgtttatttt tacagacaaa atttcttgta
    22201 aagattggtt atcagcaggc aaatattgct gaaacattga aaatccatcc atatcgtgta
    22261 aaacttgcca tgcaggaagt aaaaaaattc gatgaacatt tgctggtaca gttgtttgat
    22321 caattagttg agatggatta tcaaatcaaa acgggacaaa tcgaaaaaga attaagtttt
    22381 caactattcg ttttgcgcac agggcagttg ttaagaaatc gataagaaga aagacgaggg
    22441 caaagcatgc gggaaggtcg tttgtttttg tccttttttt gacaaataaa aaaatcggcc
    22501 gaagccgatt cttttattta gcgatttttt tgcttaaacg agatttgtcg cggttagctt
    22561 tgtttttgtg gattagacct tttgattcag ccatatcaat agctttaaca gcttctttat
    22621 ataacgcatc tacgttgtca gcacctgaag ctacagcatc ttcaaatttt ttgattgcag
    22681 tacgcatagc gcttgtttga gatgaatttt tcacattagc gttttcgctt gtacgaacac
    22741 gtttgattgc agattcaatg tttggcatta gtttcacctc cgataactta agttcttacg
    22801 cgtgttaccg cataattcaa cattcattat tatacctaaa ggcagcgtgc tttgcaatat
    22861 gaacatgaca agtttttttc cttaacagtc ttttattagg attttttttc agtaaaaacg
    22921 gatagtacaa gctgaaaaaa atcaaaaaga cgctattgtg ggaggcaata gcgtcaaacc
    22981 aaactagatt tttatttatc tatttaaaca aagtttgaat gggaatgttt ggtactaagt
    23041 tattatataa caaagagaac cgtttgcaaa gttttttttg aaaagggata cgattccctg
    23101 ttgttctttg tttttctatt aagaaactgc agttatttta taaccagtat cctttgatca
    23161 gtaaaggcta ttctggtgta ccgattattt taatgtattc atcgatattt tttatcaaca
    23221 ttttttttat gaccaactgg ttccgacggt tccatttgcc acttacttcc agcacatatt
    23281 tatttagggg caaacgtaag atatattttg ccaaatagcg atctttaagc aggccattga
    23341 tcacacggtc ctcaagctgc aagttgatcc cagtcagcat cattggctgt tcttgtaaaa
    23401 cgtacgtatc aattaaaaga ccttcaaaag tttccacaag cgtcttcctt tcttttcaaa
    23461 gtaacctgaa aacaggcact aattcttcca ttttgatatt cgttgaagtg gatgcgatca
    23521 ttctttcttt tgtccactgt aatctagtat aacaaaaaag agcagaagag ccaaaaagaa
    23581 aaaagagcat cgaattctat aggaagtctc attggcacgg cctaaattat tacaaaacat
    23641 tgattaaaaa aagcccctgc atcgcaggag catcccctat atcgttttaa taggtcaatc
    23701 aaaaagcagt atcaaacaca ttttgtatca aatcaggaaa taatagataa gctaagccca
    23761 gaatcaaaag taccgctaga agaaaccagc cgattgttcc tatgaaatac tttaattttt
    23821 cgaacattgt atcacctcgt tgtcagtata tcacggatca gtaaattggg ggtgttaaga
    23881 atctcctaaa atagacaaaa tttgttctat tttcctaaag caaatacttg taagatacag
    23941 taagtgatac cagaagcaat gaccggtccg gcggcgattc ctcttaagaa gacaacacct
    24001 aaaatcgtgc cgaaaacaag tgcaacagtg atttgtggat cagaagcaat caatcctaca
    24061 cccttggaag ataagacagc cactagccca ccgcaaaaaa cagctaccca gccaataggg
    24121 gatttaaagg cttctaatag atcacgtaga ccgatttctc ctgttgcgat agggattaga
    24181 atagccgctg tgatgatcgt caccccaata ttgattcctt tgccttgaag ccaagggaaa
    24241 aaggaacggg aggctggtat gagcttcata atcaaaacaa aagctgccgc tatcatcaga
    24301 ctttgatttt tagctaaaat tgctacaagt aaaataattc ctaaaaaaag ccaactttcc
    24361 atgaaatata ttcctcactt tcttcgaaac aatcataaca tgtttttaga aagttacaaa
    24421 agtaggaatt catccttcaa gacctattca taagctgttt ttttcataaa ataaacaaaa
    24481 tcataaaaaa acactttgct tatttgacag aagacagccg cgctttgtat agtggacaag
    24541 acttacattt ttttgaagaa tcagaaagat caggtaaagg tcttagtaaa cgcaagccat
    24601 tcagaataac caatattgtg cttccttcgt gtcctacaac tccaagaggt agatttaaaa
    24661 attgcaggaa gttagaagca atcaacaaga gaataacaag aatcgaaaaa ataatatttt
    24721 gtttgatgat ccgccgcatt tttaaagata gccgatgact gtagacaaat ttatttaagt
    24781 cgcttttcat taagacgatg tctgcgatat ccatagcggc atctgtccct tgccccatag
    24841 cgacacctaa agaagcggct gctaaagcag gcgcatcatt gactccatca ccaaccatcg
    24901 catttacttg gtaaagttca ttctcttttt tgactagttg tgttttttct tccggagtac
    24961 agcttgcata gtaatgttca atccctactt gttcagcaat ggcctttgct gtgccagaat
    25021 ggtcgccggt tatcatactt gtatggatgt tttgggattt aaagtaggaa attacttgtt
    25081 gggtatttgc ttttggaata tctagcagac ctaaaacagc aattaactgc tgatctttag
    25141 aaagataaat cacggttttg ccttgatttt ccaaacgttc gatcatttcc gcaatctctt
    25201 tgctgattat catcgtctca ggatcatatg cgtgcttgcc aactttccat tgcgcaccta
    25261 aataggtagt ttgtaatcca aaaccagtaa tttcttctat tggtaagtat tggatttctt
    25321 caggtattgt gcaatcgaaa tattgcaaaa tcgcatgagc aagtggatgc gtcgtttttt
    25381 gttccatagc aacaagaagt tgttgtgcca aatccttttc atcaaagaaa aagctgtcag
    25441 ttacaactgg ttttccttta gtgagcgttc ctgttttatc aaaggcaatc gcttttaatt
    25501 cagaaagctt ttctaaatgg ataccgcctt tgattaaaat cccatttttt gcagaatttg
    25561 aaagagcagc tagagaagct ggtgtagcag atgcgacaag cgcacaagga gaagccacaa
    25621 ctaataatac cattcctcga taaaagcttt catcccaact ccaacctagg aaaaagtggg
    25681 ggagaaggat cattagagga acagcaagca aaatcaattt tacataatta ttttcgattc
    25741 gttcgataaa tgaagctgtt tgagttggcg tattctgcgc ttcttctact aattggataa
    25801 tcttagcaaa gacagtttcg gaactcgttt ttgttactcg aatcgtgatg ggatttcctt
    25861 gattcagcgt tcctgcatac atctcatcgc ctaaatattt ttctacaggc acagattcac
    25921 cgcttaatgc agactcatcg atcgttgcct gtgtgctttc taaaaagcca tctatcggaa
    25981 cagctgctcc tttagcgaca aataccaagt tatccacctt tagttgagaa acatctactt
    26041 cttgcatttg tccattttct tttaataatt gggctttttg tggttgaaga ttcatcaaag
    26101 cagtaatttc tttttgactt ttattagttg tgtattcctc taatgcaccg cttaaacaaa
    26161 agatgaatgt caacattgct ccttcaaagt aattaccgat caaacaggca ccaatagcag
    26221 ctaaagccat caataaatcc acattcagtg tccgatcatt ccacaattct ttcaagcctt
    26281 ccgatgtttg tttatatcca ccgatgataa tggcactgat ataaaaaagt ggggtgatgg
    26341 taagagagaa tcggtcgaag ataaaaccga agatcataaa taaaaggcag ccgattgttg
    26401 ctgcaaaagc ttttttctga ttagtcatcg ttattagacc tccttgtcaa aacaaggata
    26461 gcataataaa aatgtaattg ataataataa cagctaaatg agaacgataa tggttatcat
    26521 ttggtgatta agcttgatgt tgtttttatt atttataatg attattatta aagaaaataa
    26581 aaaaccagaa catttctcaa ataagagaag tgttctggtt ttttctacat tgcaataaac
    26641 caataaccaa tcaaaatgat accaaggata atacgatacc agccaaaagc ggtaaagtcg
    26701 tttttcttca aatagcttag taagaatttg atagctaaga cggaaaccac aaaagatacg
    26761 aatgttccta caagaagaac aacagtttct tccattttga atgaattccc ttcagctaaa
    26821 tatttgacga tcttcaagcc gcttgcgcca aacatgacag gaatccctaa gaaaaatgaa
    26881 aactctgtag cgacaaaacg cgaagcacca atcaagatag atcctaggat cgttgcgcca
    26941 gaacgggatg ttccgggaac aagtgccaag acttggaata acccaacaag aagagctgct
    27001 tggtacgtaa agtcattcag agaagtccaa cgaggtgttt tgtttttgtt gcgtttttcg
    27061 acaataataa aggcaatacc gtaaacgatc aaaacaatgg caactggtaa gaacttgtgg
    27121 aaatttgctt ctaaccaatc atccaaaggc aaaccaatga tgatggctgg tatgcaagca
    27181 acgatcactt taaaccaaag tgtccacgta tctttttttt ctaccgttga tttacttgga
    27241 gaaaatgggt ttaacttgtg aaaataaaga acgacgacag ccaagatcgc ccctaattgg
    27301 ataacgacat tgaacatcga cataaattgt gaactggcat ccagtttgat gaattcatct
    27361 gctaggatca aatgaccagt actactgatg ggcagccatt ccgttattcc ttcaataata
    27421 ccgagaatca ctgctttgat aatatttgca aaaaacataa attccttcct tcccaattaa
    27481 gatacatctt atagtatacc gttatttttt gagaaaccca atctgttttt taatattaag
    27541 aaaaacttga gaaataaagc agataagtac acgaagagaa aaaagaagac aaagccctcg
    27601 ttcagacttt gccttttatg attctctggt aaaaattaaa atatccccat tatagaaacg
    27661 agtcgcgact atgtgttttc ccgttatcga tataagacgt atcattgatg ccgtggatag
    27721 aaaatcgacc gtttgcataa gtgacctttg ttacgctgcc atttaaaagc ccagcacgaa
    27781 caggctgttt aggatcgatc agattaagca aaaaggcgat cgtcaaacca tgagatacga
    27841 ctaaaacatt tccaccacca tttttttcgc agtgatgggc gatatcttca aatcctgtcc
    27901 atacacggtc tcgcaatgtt tcataagttt ccgcccaccc agcggtatct aagttgtata
    27961 cagcattagc aatttgttca aaagaaactt ctgtggtaaa catttcatca tatgttttaa
    28021 aattcaatac acgagggatc acaccccaca tttccatgtc atatccacct tcaagagacc
    28081 cgaaacacca ttctctaatc cttttttcaa taaaataagg aatttctttt cccttagaat
    28141 gttctcctag aataatacgg gcagtttcga ttgcacgacc actgtcacta gaataagcaa
    28201 atgaaaagtc gacatttttt aacccgcgac ctaaataatg aatgccttct tgtccttgct
    28261 ttgtgagggg tgtatcacac cagccttgta ctcgttcaat tgtattgaac atcgtttttc
    28321 cgtgtcgtac gatataaagt gtagtttctg tcattttatt ctcctcctaa gtcgttttaa
    28381 cgattgaaaa aaggatttgt ccttttttcc tgttcaatcg tcgttgcatc gccatgtcct
    28441 ggatagactg caaattcatc aggtaataca aacagctggg tccgaatact atgaagtaat
    28501 tgttctaaat tccctgtata cagatcggtt ctaccgatac ttcctctaaa cagggcgtcg
    28561 ccagaaacga caaaatcatc aaaaataaag ctgacgcttc ctatagaatg ccctggggta
    28621 gggacgacgc gaaaagacat gtttcctaaa gtataagttt tcatttcaaa ttcatattct
    28681 gcaggcgaga caataacatc agcaatatca tcatgtctac cgagtccaga aagattcaaa
    28741 atcggatcac ctagccattt ttgctctagt gggctgacat aaactgggat ttgataatac
    28801 gcgcgtaaag gttccactgc accgatatga tcgtagtgag tatgggtaag caaaatcgct
    28861 accggctgtt ggtttgtttt tttgatttct tgtttgattc gttccgcatc ttctccaggg
    28921 tcaatgatca aaagtgcttc ttcgttgtag accaaataac agttttcttc aatcattcct
    28981 gtttttaatc tttcgatatg catgtgcatt cctccttgat agccaaaact tctttcatcc
    29041 attagtataa cgcattcatt gctaggaaac aaaaacaggt acttatttct ctttacttat
    29101 ctgaattatc agactatatg aaaagtattt ggatattcta aagagaagga aagtaagttt
    29161 tcatagcatt ttaatatgtc cttgttacat tagattcatc caaaaatcca aattctatat
    29221 cctatatccc tgcctaatca gcagggtgtt tttttatgtt tggaaagaga gctctttttg
    29281 ctaaactagg ttttttcagg taatcttaga gagaaagagg tggtggaaat gatcaaacag
    29341 tcaaaaatat ctgtgcggca cttagtagaa tttattttgc gacgtggaag tatcgacaat
    29401 cgtaaaaaaa gtaaccatac tgcattagaa ggagcgagaa tccaccgcaa gctccaaaaa
    29461 gatgcgggag aaaactatga aaaagaagtc tttttaaaaa caacagttca attagatgaa
    29521 tattacctga tcgttgaagg ccgtgcagac ggtattttca aaagagacgg tatttattat
    29581 atagatgaaa tcaaaacctc cgaaccacga tttgaagatt tagaaccaga acaagtggaa
    29641 cttttctttc atcaagcaag ggtgtatgcc tatatctata gtcaagaaca tgatttacaa
    29701 gaaataaatc tgcaattgac ttacttccag acggcagaag aagtgatcac gagaaaggtt
    29761 gaacatcaga caagagagca gttagaaact ttttttaaaa agctgactga agattataaa
    29821 gaatggcttg tttttcaaga aaactggcgc actgtacgca atgcctcttt gatggctttg
    29881 aaattccccc atgatgagta taggaaaggt cagcgtgaac tagcggtagc tgcttataaa
    29941 acgatccgta cgaaacagaa attgtttgta gaagcaccta ccggaacagg aaaaacaatc
    30001 tctacgttat tccccgcttt gaaagctgta ggggaagaag agggagaaaa gatcttttat
    30061 ctaacggcta aaacaataac tagacaagta gctgaagatg ctatgacagc attaaaagat
    30121 acaggagctg aagtaaaaag tgtcactttg acggcgaaag acaagatctg ctttttagat
    30181 gagacgacct gtaatccaga tcagtgccca tatgccaatg gttattacaa tcggattaat
    30241 gaaggtttat gggatctttt gaatcatgag aatcagatca ctcgtgaagt catcgaaact
    30301 tatgcaagaa agcatacact ttgtcctttc gaactatcat tagatgtcag tatctggtgt
    30361 gacgtaatca ttggggatta caattattta tttgatccaa ctgtttattt aagacgtttt
    30421 tttgaagatg aaaaaaacga ggactatctg tttttgattg acgaagcaca taatttggtg
    30481 aatcgttccc gagagatgta ttcggcagaa ctatcttatg aaaaaacgaa acgaagtaag
    30541 gaagctatac caaaggaatt caaaaaactt catcgccgtt tcaacaaact attaaaagaa
    30601 tttgattcga tccgagaaat agcaaaagaa gatcattggg attatcatca tcaaaaagca
    30661 ccggctgaat ctttagtaaa ggcaggttat caattaagcg aaaaaattaa agaatggctg
    30721 gcagaatttc ctgagcaccc atcacaagaa caattgcttc cttattattt tgatttgctt
    30781 cattttttga aggtcagtga attttatgat gatcactacg aaacaacagt cgaaaaaacg
    30841 tatcgcgatc tgatagtaaa agagttttgt attgatccaa gcttgtttct tgagcagtcg
    30901 ttggataaag gaaaaagcag tattcttttt tctgctagtt tttcaccact ctcttattat
    30961 caggaaacac tggggggaca aaaaagtctg gcttacaaac tgccgagtcc tttcccagaa
    31021 ggaaaccagc aagttttcat cgcaaattac ctagagacga cttatcgaaa aagggaacac
    31081 agtctggcaa aattagtaga gacgattcat gcttttgctg atggaaaggt cgggaattat
    31141 ttcgttttct ttccttctta tcaatattta gatcttgcgg tggaagcatt cagacagcaa
    31201 tatcccgaca gcaatgtgct aatccaagaa acggacatga atgaggaaga acgcgaacga
    31261 tttttaacaa aatttcaaac aaatcccgct gaaacattga ttggattttg cgtacttggc
    31321 gggatttttt cagaaggaat cgatttgaga ggggaccgat tgatcggcag tatgatcgta
    31381 ggtgtaggtt tgccgcagat gaatcatgag caggaactga tcaaatcata ttatgatgag
    31441 aaggaacacc ttggatttgc ttacgcttat cagcttcctg gaatgaacaa agtcttgcaa
    31501 gctggtggtc gagtgattcg tggcatggcg gatcaaggaa tcgttgtatt ggcagatcaa
    31561 cgattttcta gtttttctta tcgacgttta ttcccacaac attggcaaac agcaagagaa
    31621 gtacgaagtg ttggggaatt ggaagaagga atacagcgtt tttggttatc aaaagaagca
    31681 gtaaatcaaa aaaacgagga atgacagacg tcagcctcgt tttttgatta acgatagtta
    31741 gtgaattgca actcgattgg taagtcaagt ttacgtaata aagcaatcac agcttgtaga
    31801 tcatcacggt tcttacctgt cactctaatc tgatcttctt ggatttgtgc ttttactttc
    31861 agtttttcat ttttgatagc tgtagtgatt ttcttcgcgt tttcacgatc gattccgttg
    31921 accaaatcgg cagtttgtcg agctttgcca cctagagcgt gttctgattc ggaaaaatgg
    31981 atatttttga ttggcacatt tcttttgatc agcttaccaa ataatacatc tttgatctgt
    32041 tcgattttga aatcatcatc gctcaataca actagattgc tgttctctaa tttgatttct
    32101 actgtagaat ccttgaagtc aaagcgattg gacaattctt ttgttgtttg ctgaatcgca
    32161 ttttttactt cttctgtatt gacttctgat acaatgtcga agctagcatc ttttgccata
    32221 ggaaccctcc tgatatacat ataaatattt accatacatc tatcttatca taggattagg
    32281 gcagattctt acctgtttgc taaaattctt ctcatttgag ttcgaagtat tttattttaa
    32341 tagataaact gttattctta catttggaat accaaagtag gggagaggtg cttgtgagta
    32401 aaattatgac tgcgtatcat aaaaatgaaa gattaaagaa aatagtggtt attttgataa
    32461 ccggattgac cgctgcggtc gctttaaatt attttttgat tccagccaat gtcttttctg
    32521 cagggatgaa tgggatcgct caaatcattt cctcgctttt atatgaatgg tttcatatca
    32581 agatagacac aggaacattt atttttcttt tgaatatccc tgtgtttgta ttagggtttc
    32641 tcaaagtagg aaaacgagca acgatcctta gttttgtgaa tgttgtatgt gtatcggtga
    32701 tgacgatgct gcttccagtc ggacagatca ctgataatat tttgatgaat gccctagtag
    32761 gcggtgtttt attaggtctt ggcgtaggat tatctttaaa aatgggcttt acaactggtg
    32821 ggatggatat catctctctt gtactttcac aaacaactgg aaaaacagtc ggaaactaca
    32881 tgctgattct aaatggtatc attgtagtca tcgcaggctt cttctttaac tgggaaagtg
    32941 ccttgtatac gattatttcc atttatgcaa tgactcaagt aattgatgcc atccacacga
    33001 gtcatcaaaa agtaacagca atgatcgtta ccgtcaagcc agaagaggta acagaagaac
    33061 tagcaaaacg gatggtacgt ggaatgacat tactgccgtc tgtcggtggc tattcaaaaa
    33121 aacaagggaa catgatcatg atggtcatca cccgctacga aatgtatgat cttgatcaga
    33181 tcgttcatga aatcgacgac gatgcattta tcaatatttt accaactcat tcagtatttg
    33241 gccggtttgc aaatgaaaac gaacaaaaaa tgtatcgaac aactggcgta ttgccagaaa
    33301 taaaaaatcg ttcacaaaaa aaatgaggaa tcaggtttat tttagaataa atttaatcta
    33361 ttacatctaa taagaacatt tttataaatt taaacgagaa tcttacttct aaattctcat
    33421 ttacaagcag ctaaaaaaag agtatgataa ataaaaatcg tgttaaggag tgtcgtacga
    33481 tgagcgaagc aaaccaagaa cggtctggac aagaaataga actaatcaaa gaacgtattc
    33541 ttgctgcctt ggaaatggtg attgatcctg aattaggaat cgacatcgtc aatttaggct
    33601 tgatttatga tatagaattc aatcctgaaa atggagaaac agtcattaag atgactctaa
    33661 caactatggg ctgtccatta gcagatattt taacagaatc tatccatgac gcattaaaag
    33721 aagtgccaga agtaacgaaa gcagaagtca aactagtctg gtatccagca tggacgacag
    33781 ataagatgag ccgttatgca cggattgcgt tggggatacg atagaaatct tggctgacta
    33841 gctatgagaa gcaaatagga aaaacaaaaa agccttttat cctttctaga agaaaagaga
    33901 taaaaggttt ctttgtttat aaaattttgt ctgctatatt ataagttaat taaataaatg
    33961 tgttctattg ataaaggaca ttcaaatccc tttccttttg gaatccttct tttttacatc
    34021 agtttgattt tcgtatagta ttctttctca tcttcacgat aaatcgcagc cccatttttt
    34081 tgttgtgctt taagactgcc aagatttttt ttatttacag acaaaagtgc ctcatcaatg
    34141 ccgatttttt ttgcctcttg tagtactaat gctaatcctt gtgtggcata tcctttttta
    34201 cgataattag gggagatgcc gtaaccaata tggccagcgc cgtttcttaa tgtttcgttc
    34261 agtttatggc gcaaattaaa aattcctaca tactcatgtt catcactttc taaaataaag
    34321 acggttgcgg gaacgtaact ctcaggaaga tcgattccta aagacgtttt ttcgcatttt
    34381 tttacataat ctaaaaattc agcaaatgta tagccataag cattgttgat aaatccactc
    34441 tcatttggtc caaagctagt ttgtagctga taagctttct ttaaatctgc ttcccatagt
    34501 ttgatcagtt tcatcgtatt cacctcttta agtaacctta ttcatttgtt ttctactata
    34561 gcactattgt tgtaaatagc catgcctatt tttatttatt gtgtcatatc gtattaagaa
    34621 tcatcgaaca atacaaatat gaattacaat aaaaaaatta tttgaagttc gttcattcgt
    34681 tacttatgat ctctagaaga aagaaaacgt gaattacttc agcgtctaga attccagaag
    34741 gatagtaatc aacagaagaa ataatcaata tccaaatgaa agaagatagc tgaatcgaga
    34801 aaaagaggaa aaaagaaatg aagaaaaaaa taaaatagtt actaatccca gagtcgttcg
    34861 tttaacattg aaatcgatca tatggataaa acagattttt tcaccaaccc tcaatagatg
    34921 atggagttgc tcggatgaat tatttgtgta gatggcataa gggagggcgt acagaaatga
    34981 aaaagaacaa aaatatgttc ttgacaaaaa acacatgtaa tcctaaacta aaagatgtga
    35041 tgcggctatg gcggaattgg cagacgcgct agcttcaggc gctagtgagg gtgacctcgt
    35101 ggaagttcga gtcttcttag ccgcataatt tgttttttta taaagagaag gtacacctgg
    35161 agcgatgaga aagaaaataa catattatct aaaagaatat aggaaaaatt ttaaacaacg
    35221 gaaccagatt caaataaatc aaaaaaaatt tgtgtgaagg tgggacagtt taaaacctaa
    35281 tagattgatt caaatagtag atttatagat tctgatacaa gaaatactgg caaacgatag
    35341 ttaaggaata aagactaaac ttaaactcaa aaagaggtca tgcgaaagaa taaatcgcat
    35401 gacctcttac tatattttga atgtgtttaa acgttcaaat aatgaatctt agtaccaacc
    35461 gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca taacgtgaag taacatattg
    35521 ttctgctact ctttcttggt ttgcagcaga gtagtcacca tttaagtaag aagaatctaa
    35581 ttggtaacgg ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg
    35641 tgcgatccat tcttttgcag agcttgtact tgctggagta gcagcagttt ctgttacagc
    35701 aggagcaggt tgtgtttcag caacaggcgc tgcttgtgtt tccgtttgaa ccgtttcttg
    35761 tacaacaggt tgttcagctt gaacaggttc agcttggact ggtgcttctt gaacggctgc
    35821 ttgaggttca gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga
    35881 gta
//
LOCUS       DACCCR010000025        34642 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCCR010000025 DACCCR010000000
VERSION     DACCCR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.026"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            598..1287
                     /locus_tag="IVK93_001188"
     CDS             598..1287
                     /locus_tag="IVK93_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6386489.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="IVK93_001189"
     CDS             1274..2482
                     /locus_tag="IVK93_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6386490.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEAERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="IVK93_001190"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="IVK93_001190"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP6386491.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IVK93_001191"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IVK93_001191"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP6386492.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="IVK93_001192"
     CDS             complement(6585..7280)
                     /locus_tag="IVK93_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP6386493.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IVK93_001193"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IVK93_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP6386494.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="IVK93_001194"
     CDS             complement(8479..8670)
                     /locus_tag="IVK93_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP6386495.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="IVK93_001195"
     CDS             complement(8691..9581)
                     /locus_tag="IVK93_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP6386496.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="IVK93_001196"
     CDS             complement(9568..10335)
                     /locus_tag="IVK93_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6386497.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IVK93_001197"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IVK93_001197"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP6386498.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IVK93_001198"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IVK93_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP6386499.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IVK93_001199"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IVK93_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP6386500.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14951..15289
                     /locus_tag="IVK93_001200"
     CDS             14951..15289
                     /locus_tag="IVK93_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386501.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="IVK93_001201"
     CDS             complement(15457..16215)
                     /locus_tag="IVK93_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6386502.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="IVK93_001202"
     CDS             complement(16233..17051)
                     /locus_tag="IVK93_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6386503.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IVK93_001203"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IVK93_001203"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP6386504.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17521..17655)
                     /gene="rpmH"
                     /locus_tag="IVK93_001204"
     CDS             complement(17521..17655)
                     /gene="rpmH"
                     /locus_tag="IVK93_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP6386505.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18178..19512
                     /gene="dnaA"
                     /locus_tag="IVK93_001205"
     CDS             18178..19512
                     /gene="dnaA"
                     /locus_tag="IVK93_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP6386506.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19710..20840
                     /gene="dnaN"
                     /locus_tag="IVK93_001206"
     CDS             19710..20840
                     /gene="dnaN"
                     /locus_tag="IVK93_001206"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP6386507.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21069..21311
                     /gene="yaaA"
                     /locus_tag="IVK93_001207"
     CDS             21069..21311
                     /gene="yaaA"
                     /locus_tag="IVK93_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP6386508.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21298..22422
                     /gene="recF"
                     /locus_tag="IVK93_001208"
     CDS             21298..22422
                     /gene="recF"
                     /locus_tag="IVK93_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP6386509.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22419..24365
                     /gene="gyrB"
                     /locus_tag="IVK93_001209"
     CDS             22419..24365
                     /gene="gyrB"
                     /locus_tag="IVK93_001209"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP6386510.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24385..26856
                     /gene="gyrA"
                     /locus_tag="IVK93_001210"
     CDS             24385..26856
                     /gene="gyrA"
                     /locus_tag="IVK93_001210"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP6386511.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27092..27391
                     /locus_tag="IVK93_001211"
     CDS             27092..27391
                     /locus_tag="IVK93_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP6386512.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27441..27953
                     /gene="ssb"
                     /locus_tag="IVK93_001212"
     CDS             27441..27953
                     /gene="ssb"
                     /locus_tag="IVK93_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6386513.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27979..28215
                     /gene="rpsR"
                     /locus_tag="IVK93_001213"
     CDS             27979..28215
                     /gene="rpsR"
                     /locus_tag="IVK93_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP6386514.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28358..30331
                     /locus_tag="IVK93_001214"
     CDS             28358..30331
                     /locus_tag="IVK93_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP6386515.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30337..30789
                     /gene="rplI"
                     /locus_tag="IVK93_001215"
     CDS             30337..30789
                     /gene="rplI"
                     /locus_tag="IVK93_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP6386516.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31063..32430
                     /gene="dnaB"
                     /locus_tag="IVK93_001216"
     CDS             31063..32430
                     /gene="dnaB"
                     /locus_tag="IVK93_001216"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP6386517.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33210..34502
                     /locus_tag="IVK93_001217"
     CDS             33210..34502
                     /locus_tag="IVK93_001217"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP6386518.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6735 c         6845 g        10508 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaagc
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtat attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa accactgaaa ccccagtggc
    17521 ctaagctgaa agaacttttc ttcctttgcg acgacggcta gctaacacac gacgtccatt
    17581 tttagtactc atacgtttac ggaatccgtg aactttttga cgtttacgtt tatttggttg
    17641 gtatgttctt ttcatatatt ccacctccaa aatgttctta tctattttat aaacacagac
    17701 atactttgat agtataacga caaagtagtg attttgtcaa tataaaatag aagatcgatt
    17761 gttaaaactt atccacaaga ttattttttt ctatgggaat cctatgtgga aaaagttatg
    17821 cacagggatt atccacagat aataaaaggc tggttttaca cagaaaacac acgtgtaaaa
    17881 aaagtaatcc acaatcaggc gatttcctgt gtattccgtc tcctaactat tggtatatct
    17941 gtatttttta tccacagacg caagaggaaa tctattggtt tttacgagtt ttccacctgt
    18001 gcattagtgg tgtggaaaac tttttttgac aactgtgttt ttctcttttt tctttttgtc
    18061 gattgtgtgg ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg
    18121 aaaaaaatgc taaattagta ctagttattc actttttaat gaaggaggag gccttatatg
    18181 gtatccctcg atgctttatg gaatgaatta aaagcaacat accaaaagga tttatcgcct
    18241 gctagctata atacatggat cgaaacagcg aaccctcgaa cccttgatca aaaccagctc
    18301 gttgttgagg ttcctagcaa gattcataaa gaatattggg aaaaaaacct ggcgacaaaa
    18361 atcgttgaag tcggttatat gttatcagga aatgagatta tccctcgctt tattactggc
    18421 gaagaagcag agcaagaaga agttatagaa gaaaaaaatc caaaagttgt ggcacctagt
    18481 ccgctaaaaa aagccatgct gaaccctaaa tatacctttg atacgtttgt cattggtaaa
    18541 ggaaaccaga tggctcatgc agcagcgctc gttgtagcag aagatccagg ttctatttat
    18601 aacccgctct tcttttatgg aggcgtggga cttggtaaga ctcacttgat gcatgcaatt
    18661 ggtcatcaaa tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag cagtgaaact
    18721 tttgctaatg atttcatcaa ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa
    18781 tatcgaaatg ttgatttact gctagtggat gacattcaat tttttgcaga aaaagaagct
    18841 acgcaagaag agtttttcca tactttcaat gccttgtaca atgaagggaa acaaatcgta
    18901 ctaaccagtg atcggttgcc aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc
    18961 tttgcatggg ggctgtctgt tgatattaca ccgccggatc ttgaaacaag aacagcgatt
    19021 ttacgtaaaa aagcggcggc tgaacgtcta gagatcccag atgacacact aagctacatc
    19081 gcaggacaaa ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct
    19141 tttgctgcga tgaatagtga agatatttcg actagtctag ctgcagacgc actgaaaacg
    19201 ttaaaatcag gaaaaggaca tcctcaattg tcaatcctgc agatccaaga agaagtggca
    19261 aagtattatc acatccaatt gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt
    19321 gttcctagac aaatcgctat gtatttggca cgtgagctca cagataattc tttaccgaaa
    19381 atcggagctg aattcggtgg caaggatcat acaacggtta tccatgctca tgaaaaaatc
    19441 cagcagctga tggattctag tgtcagtatg caaaatgaag tatcagaaat caaaaacttt
    19501 ttactaaatt aatttgtcga ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa
    19561 cgttcaaaag ttatacacac tttttccaca gctgtttttt cgtttctctc ttacgttttt
    19621 tgagttttcc acagaatcaa catgccctat tacttttatt attcttttta actaataata
    19681 aataaacaaa cattcaaagg agatacatca tgaaagttac tttaaaccga gctagcttta
    19741 tgcaggaatt gcaaactgtt caacgagcta tttcaagcaa aaccacgatc cctattttga
    19801 caggtgtaaa aatcacactg acacaagaag gtttgacttt gacggggagc aacgctgata
    19861 tatcaattga aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta
    19921 ctggttccat tgttttacaa gcacgtttct ttagcgaaat cattcggaga cttcctgaag
    19981 aaacatttac tttagaagtt ttagaaaata aacaagtagc gatcacttct ggaaaagcga
    20041 attttatcgt aaatggatta gatgcagata actatcctca tcttcctgtt gtcgaaagcc
    20101 ataaccagat gaaattacct gtacacgtat tgactaaact aatcaacgaa acagtttttg
    20161 ctgtctcaca acatgagagt cgtccaatct tgacaggtgt gcatttcatt ttatctgata
    20221 attctttatt agctgtagct actgattctc accgtctaag tcaacgcgtg attccagtag
    20281 aacaagcggc tgatcatttt gatattgtta ttcctggaaa aagtttgatc gaattatctc
    20341 gctcattaac aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt
    20401 tcaaaacaga aacgatgtat ttctattccc gtttgctaga aggaaactat ccagatacca
    20461 atcgtttgat tccttcaagt tttaatacag aggttgaatt ttctgttcct agctttttag
    20521 cggcgatcga acgggcttct ttactttctc atgaaggccg taacaacatc gttcgtttat
    20581 ctattcgtcc agatgcggtt gttttatacg gaaattcgcc agaaatcggg aaagtagaag
    20641 aaagcttgag ttatacagca agcagtggcg atccattaga tatttctttc aatccggatt
    20701 atatgaaagc agcgttgcgt gcatttggag atatgagcat caaagtgaaa tttatctctg
    20761 ctattcgtcc atttacattg gaaccaacgg aagatggcgt tcaatttatt cagctgatta
    20821 ctcctgtacg tacaaattaa caatgatttt caatgttctt gaaaatttaa ttgaaaagaa
    20881 atagaaaaaa taaaatttta aacagaaggc cctaaaatct cttttaaggc tttctgttta
    20941 tttttgttta aatcaccttt tttagattaa atcgtcttat atggaaaaat atatggataa
    21001 atttgtttta ttgcgaaaaa aaaggtataa tagagtatac ttttaataat gaaaatgagg
    21061 gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg ggccaaatgt
    21121 tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca
    21181 ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat cccggcatga
    21241 tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac gacgataatg
    21301 aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa catcgaattt
    21361 tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa
    21421 agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca ggaattgatt
    21481 ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc atccactctt
    21541 ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca tatcgaacag
    21601 aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc agaagatctt
    21661 tctttagtaa aaggcagccc gcagaccagg cggaagtttt tagatatgga attaggccag
    21721 atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa acaaagaaac
    21781 cagtacttga agcaattgaa tgaaaaaaaa caaactgatt tgctttattt ggatattttg
    21841 tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca gttcgtcaag
    21901 cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa agagcagctg
    21961 gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga gcaaatccaa
    22021 gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg gggaacaacg
    22081 acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa tgtgcagact
    22141 tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc agaaatcgat
    22201 ttgattaaag aagagacagg ggaatatcca atccttttgt tagatgatgt gatgagcgag
    22261 ctggatgata atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc
    22321 ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc tgagattttt
    22381 tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa agaagtttag
    22441 tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga cttgaagccg
    22501 ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg catcaccttg
    22561 tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca acgaaaatcc
    22621 atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg gggatacctg
    22681 tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact gttttacatg
    22741 ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac ggagtaggat
    22801 cttctgttgt taacgcgctt tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg
    22861 tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt atcgaacaaa
    22921 cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt acagagacaa
    22981 ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc ttgaaccgcg
    23041 ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat gaataccatt
    23101 atgacggcgg gattaaaagt tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc
    23161 cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc tctatgcaat
    23221 atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat acgtatgaag
    23281 gcggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat gattatgccc
    23341 gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac gtgcgagaag
    23401 gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga caaacaaaaa
    23461 caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc gaacatttca
    23521 tgaaattctt gctggaaaac ccaagtgtgg gccgtcaaat tgttgaaaaa ggattactag
    23581 cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt
    23641 tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat cctgaaaaat
    23701 gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc
    23761 gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca
    23821 tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg ggtacagggt
    23881 ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc atgactgatg
    23941 ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat cgttacatgc
    24001 gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac ggggtaaaac
    24061 aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag
    24121 tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa ggacttggag
    24181 aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg atgatggccc
    24241 gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg atgggggatc
    24301 gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag aacttagata
    24361 tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat ccatgatgtc
    24421 aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt tattgtcgca
    24481 cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat tttatacggc
    24541 atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag aatcgtcgga
    24601 gatgtcatgg gtaaatacca tcctcatgga gactatgcga tctatgagtc aatggtacgg
    24661 atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa ctttggttct
    24721 gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag caaaatcgct
    24781 acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa ctatgatgat
    24841 acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt gaacggaaca
    24901 acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc tgaggtagta
    24961 gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt gatggaagta
    25021 ttacctggac cagattttcc aacaggtggt ttggttatgg gaaaatcagg aatccgaaga
    25081 gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat tactgagatg
    25141 ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa taaagcaaaa
    25201 ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat cacagatttg
    25261 cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg tgacgccagc
    25321 gcatctgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc atttggattc
    25381 aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca aattcttgaa
    25441 aactatatcg aacatcagcg cgaagtcatc acaagacgga cagtctttga aaaagaaaaa
    25501 gcagaagcaa gggcacacat tttagaaggt ttgcgtattg ctttggatca tatcgatgaa
    25561 atcattgcga ttatccgcgg gtctaaatca gatgacgaag caaaagcgac gatgatcgaa
    25621 cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt acgccgattg
    25681 accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa actgattgaa
    25741 gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa gaatgaatta
    25801 gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt cggtgaggtc
    25861 ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac attgacaaac
    25921 aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag gggtggacgt
    25981 ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt ttcttgttct
    26041 acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc aaaaggatac
    26101 gaaattccag aatacgggcg cacagccaaa ggaataccaa ttatcaattt actaggtatc
    26161 gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga agaaggccac
    26221 tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct
    26281 aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat
    26341 gtgctactga cagatggtca atctaatatc attattggaa cacataacgg ctactctgtc
    26401 actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt tagaggaata
    26461 cgcctgcgtg aggaagatta tgtcgttggg gcagccttaa tgaaggaaga tcaagaagta
    26521 ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata tccagtgaaa
    26581 ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg tccactagct
    26641 ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa aggtgtcatt
    26701 atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg cgtacgtttg
    26761 atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc agaagctgca
    26821 gaacaaacag ttgaagaaca aggatcagaa gaatagccat caagactaat tgtgtaagct
    26881 agcaagaatg accgattaaa aaatctggtt attcctgctg gcttttttga ttagttgaat
    26941 ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat
    27001 aatcagagct tgtgagtagt ctaattaatt agataactct ccttgctctt aggaacctaa
    27061 gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg aaatcatgta
    27121 cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc
    27181 aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa aacgccgttt
    27241 cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag tatcttctcc
    27301 atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg acgatatcat
    27361 tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac aatagaaaac
    27421 gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt ctgacgaaag
    27481 atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact ttggctgtga
    27541 atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac tgtgtgattt
    27601 ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt ttaggtgtta
    27661 ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta tacgtgacag
    27721 aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa aatcgccaac
    27781 aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga aacaataact
    27841 ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat acttctgacc
    27901 cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc taaacgaatt
    27961 tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta
    28021 gactacattg ctgctaacca tattgaatat atcgattata aagatatcga attactaaaa
    28081 agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac aggcgctaaa
    28141 aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc
    28201 gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat cagctattca
    28261 attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg acgaaagtta
    28321 tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa acaatcattt
    28381 atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg tagtcttaat
    28441 tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat ggggtgttcg
    28501 ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc
    28561 ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg taggagtgat
    28621 tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat tgaaaatcaa
    28681 agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg caaaagaaaa
    28741 aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg atacagtaaa
    28801 gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc atcagacgaa
    28861 cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca ttgataaatt
    28921 ggatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt ctgactggat
    28981 gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct tcgtagcaca
    29041 gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc gctttcgagc
    29101 agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt atggctcaag
    29161 aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt tagtccgtgg
    29221 aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt attatggggg
    29281 gaaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt
    29341 gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt ttccggatat
    29401 ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc
    29461 ttacatcgtt atcaacgaga acgagatcat tccagatgta gaacggtgtc ttgaagaaat
    29521 ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca tagagctgaa
    29581 aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat cgatctctca
    29641 gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc gtggggaaga
    29701 gtttccaagt aaacctctgc tgacttatat cgaatcttca gcttcttcag cttcagagct
    29761 agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc gttttgaggc
    29821 cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc
    29881 acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat ccttagtaca
    29941 atatttattg agctcggatt tagcgacata cttggagatc agcaatttga ttgctggaag
    30001 cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag agtataatag
    30061 tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca atgcagcttt
    30121 cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta ccggcacgat
    30181 caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa acgcagctac
    30241 tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg caatcaaaga
    30301 aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt tttacaagat
    30361 gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta tgcacaaaat
    30421 tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca
    30481 ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc taaaaagtta
    30541 aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc aggggaagac
    30601 agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa caaacaatac
    30661 aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc actaggctac
    30721 acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta
    30781 caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga aggaaaaaag
    30841 acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga
    30901 tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag aaaacgagta
    30961 taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact tttctctcat
    31021 tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga agtgtggcag
    31081 gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc tgtttttctt
    31141 gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt ttatcgtcgt
    31201 ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga agcgattgac
    31261 gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc cggtggttta
    31321 agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt ctactatgcg
    31381 aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac aggaatcgtt
    31441 acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc cgaacgcagt
    31501 atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc tgaagtactg
    31561 aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat cacaggtcta
    31621 ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga agaactaatc
    31681 attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat
    31741 gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg ggcagaatct
    31801 cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt acgtacaggt
    31861 cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct ttctcgagca
    31921 aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc gaaatgtcgg
    31981 aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt gcagttgatt
    32041 gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa
    32101 aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc tcgtggagta
    32161 gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg atcaattgaa
    32221 caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag agaaggcgga
    32281 gatgacgatg atggaaacga gccaaaaaat gacaacgtca tcgaagtcat catcgagaag
    32341 aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata caataagttt
    32401 tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg agcaatcccg
    32461 agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta cttaatttaa
    32521 acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc cgaataaacg
    32581 gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg aggaactatt
    32641 ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct cttttcctta
    32701 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa catgagaagc
    32761 agttttttga aattgctcct tgtgactcag agttaaacgg caggctcaca acctctttct
    32821 taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga aaaatatgag
    32881 aagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct
    32941 gattcacggt gttccaaaag tagcttcttc gaaaaataag tcgaaatttc acaaaaattt
    33001 gagaagcaat tttcgtacat tccttcttat ttcttgaagc tgaccacttc tgttacaacc
    33061 tcttttttaa taaccattct aaaaagataa agttcgtttt ttaagcgttt ttataaaata
    33121 aaaataaaaa tcattcgatt ttatttgaaa tatgtatcta ttattgatac aatggttcag
    33181 gattaaataa tatagaacga ggtgttcaca tgtcatctgt agtagtagta ggtacgcaat
    33241 ggggcgatga agggaaagga aaaatcacag actttctcag tgaaaatgct gaagtgattg
    33301 cacggtacca aggtggcgac aatgcagggc acacaattaa gtttaacggt gtgacttata
    33361 aactgcattt gattccttct gggatttttt ataaagacaa gatcagtgtg atcggaaacg
    33421 gcgttgtagt caatcctaaa tcattggtta aagaattagc ttatttacat gaaaaaggtg
    33481 tagaaacaag taatttgcgt atttctgacc gcgcgcacgt aatcttgcct tatcatatca
    33541 aattggacca attacaagaa gatgctaagg gcgaaaataa gattggtaca acgattaaag
    33601 gaatcggacc tgcctatatg gataaagcag ctcgtgtagg tatccgtatt gcagacttat
    33661 tagataaaga aatttttgaa gaacgtcttc gtttgaatct agatgacaaa aaccgtcaat
    33721 ttgtaaaaat gtttgattca gaaccacttg attttgacga tatttttgaa gaatattatg
    33781 aatacggaca acaaatcaaa caatacgtca cagatacttc cgttatcttg aatgatgctt
    33841 tggatgcagg caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg gatatcgatc
    33901 agggaactta tccttttgtt acttcttcta acccagtagc cggtggggta acaatcggaa
    33961 gcggtgtagg gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc tatacgtcac
    34021 gtgtcggtga tggaccattt ccaactgaat tatttgatga aacaggagaa caaatccgcg
    34081 aagtaggacg agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc tggtttgatt
    34141 ctgtagttat gcgccattct aaacgagttt caggtattac caacctatct ttaaactcga
    34201 tcgatgtatt aagtggattg gaaacagtaa aaatttgtac ggcttatgaa ttagatggcg
    34261 aactaatcta ccactatcca gcaagcttga aagagttgaa ccgctgcaaa ccaatctatg
    34321 aagaattgcc aggctggtca gaagacatta caggatgcaa aacacttgca gaactgccag
    34381 aaaacgcacg taactatgta cgtcgtatct cagagcttgt tggtgtacgt atctcaacat
    34441 tttctgtcgg acctgataga acacaaacaa acatcttaga aagcgtctgg gcgcaaatct
    34501 aaggataaaa acgacgactg aatcaggacg ttcttaccag agcgagtcga agtgtttatc
    34561 tatacgataa taataacttg aacactgttt attcagaata agggactgtg acaaaaaccg
    34621 tgtcacagtc cctttttccg aa
//
LOCUS       DACCCR010000026        34520 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCCR010000026 DACCCR010000000
VERSION     DACCCR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.027"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(133..306)
                     /locus_tag="IVK93_001218"
     CDS             complement(133..306)
                     /locus_tag="IVK93_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6386519.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IVK93_001219"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IVK93_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IVK93_001220"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IVK93_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP6386520.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IVK93_001221"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IVK93_001221"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP6386521.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IVK93_001222"
     CDS             5704..6489
                     /locus_tag="IVK93_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6386522.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IVK93_001223"
     CDS             6502..7353
                     /locus_tag="IVK93_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP6386523.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IVK93_001224"
     CDS             complement(7477..7929)
                     /locus_tag="IVK93_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6386524.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IVK93_001225"
     CDS             complement(8394..8762)
                     /locus_tag="IVK93_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP6386525.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IVK93_001226"
     CDS             8912..9394
                     /locus_tag="IVK93_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP6386526.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IVK93_001227"
     CDS             9431..10441
                     /locus_tag="IVK93_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP6386527.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IVK93_001228"
     CDS             complement(10556..11269)
                     /locus_tag="IVK93_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386528.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="IVK93_001229"
     CDS             complement(11459..12049)
                     /locus_tag="IVK93_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP6386529.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="IVK93_001230"
     CDS             12533..12760
                     /locus_tag="IVK93_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP6386530.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="IVK93_001231"
     CDS             12776..13942
                     /locus_tag="IVK93_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP6386531.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="IVK93_001232"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="IVK93_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP6386532.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="IVK93_001233"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="IVK93_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP6386533.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="IVK93_001234"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="IVK93_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP6386534.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="IVK93_001235"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="IVK93_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP6386535.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGEAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="IVK93_001236"
     CDS             complement(19155..19820)
                     /locus_tag="IVK93_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP6386536.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="IVK93_001237"
     CDS             19796..19969
                     /locus_tag="IVK93_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6386537.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="IVK93_001238"
     CDS             complement(20014..20751)
                     /locus_tag="IVK93_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6386538.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPETLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="IVK93_001239"
     CDS             complement(20753..22255)
                     /locus_tag="IVK93_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6386539.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="IVK93_001240"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="IVK93_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP6386540.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="IVK93_001241"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="IVK93_001241"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP6386541.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="IVK93_001242"
     CDS             27597..28862
                     /locus_tag="IVK93_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP6386542.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="IVK93_001243"
     CDS             28866..29597
                     /locus_tag="IVK93_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP6386543.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29732..30616
                     /gene="rapZ"
                     /locus_tag="IVK93_001244"
     CDS             29732..30616
                     /gene="rapZ"
                     /locus_tag="IVK93_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP6386544.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30613..31611
                     /locus_tag="IVK93_001245"
     CDS             30613..31611
                     /locus_tag="IVK93_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP6386545.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31645..32580
                     /gene="whiA"
                     /locus_tag="IVK93_001246"
     CDS             31645..32580
                     /gene="whiA"
                     /locus_tag="IVK93_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP6386546.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            33123..33773
                     /locus_tag="IVK93_001247"
     CDS             33123..33773
                     /locus_tag="IVK93_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386547.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33960..>34520
                     /locus_tag="IVK93_001248"
     CDS             33960..>34520
                     /locus_tag="IVK93_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP6386548.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        11453 a         5832 c         7322 g         9913 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaaa aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg aagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcgtttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gaggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc
    29641 aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg atataatttt
    29701 attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat tagtcatcat
    29761 aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta
    29821 tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat tgatcaaaga
    29881 atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt ccttctttga
    29941 agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt
    30001 atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca
    30061 tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga
    30121 cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac cacgacaatt
    30181 acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt
    30241 tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg
    30301 ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc
    30361 agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga
    30421 tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc
    30481 aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc
    30541 tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac
    30601 ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat cgtagtggtc
    30661 ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg agcagatatc
    30721 acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag agaatcgatc
    30781 gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa
    30841 ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat
    30901 acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg
    30961 attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact
    31021 cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga atctaaaatc
    31081 gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa
    31141 gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga
    31201 cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca
    31261 atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa
    31321 acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct
    31381 tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag
    31441 atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga
    31501 tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga
    31561 gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat
    31621 tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact
    31681 ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac
    31741 ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca
    31801 attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc
    31861 gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc
    31921 acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt
    31981 tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg
    32041 gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat attttctatt
    32101 tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga
    32161 acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa aattgctgat
    32221 ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta
    32281 cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag
    32341 acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt
    32401 ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag
    32461 gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt
    32521 aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa
    32581 tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt tttttaaaag
    32641 ctatacttgc ttttcttccg tcaaactata attccaactt tactggagta acataatgtt
    32701 gaaaggcttc tattgttttt taatacaatc gtataaaaag cggggagaca actgaatgaa
    32761 gattttagtt ttatacataa acttttgctt aatatagaag tcctgatata gataggatca
    32821 agtatttatc tataactgga gattaattaa aaactggaag gaactgctcg atcaaccttg
    32881 agaactgttt ttcttacttc tggtcaagat tctcgtggtt ggtggataag gatggttgat
    32941 aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca
    33001 aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa ttgggatgta
    33061 tggttggtat acgttatacc tgaacttgaa ctatcaagat ttggtttaga atttgagaat
    33121 cgatgtttat aaagggaggg caaaaaatgt gcttttttac aacttttact atcattatga
    33181 ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt ttattatatc
    33241 gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt tttctattca
    33301 cagttattgt atttatttgg agaactacag caccaccatt gccagagatt acatatggag
    33361 aatttccgtt tagtcttgaa tatgaaatag atgaggaaag aattttgatt gaagatacgg
    33421 tgattgcaga atttatagga agttttagag ggaatgtgac aactcaacct agacgaagtt
    33481 ggagtataaa acttttaaat aatagtggag agttgaaagc atttcattat gaattcttaa
    33541 taatagaaag tgaagatgta acaatcactt taagtagtgg ctctgctgga tattttatga
    33601 atgatggtgg aagcaagcca cagctcacag tccgtgatcg tattgcaggt tttatgcatt
    33661 tttctcacta tgacttagaa gaagctcgta aaatacttgc agaatatggg atcacattaa
    33721 tcaattggga atatgcgcca ccgataagaa attttttcaa tgattattat tagtcttata
    33781 aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt ttccattttt tgtttgatag
    33841 atatatagca ttgttgaagc atgggatagt ggtgccaatt ttatcaatga ggttggtcag
    33901 gcagttaaca taattggtga tgctataaat ccatttaatt ggaattggag gaattaggtg
    33961 tggaaacagt gagattatgg atattcatga aaaattttcg ttcggctttg agatatgtta
    34021 ttctggaatc aaaatcaggt tattatctga ttgacacaga tcactatact ttttggggag
    34081 gctacctttt tccgattttt aattggataa tcccccataa ggttatgaaa attaacttgc
    34141 ctgcagaaga aataaataga ttaatactca acaaagattt acaaaggaaa agagagaaag
    34201 aacattctaa tgttgcttgg ataggtccag catttgcagt agtgacttct tcaattgtcg
    34261 gtcctatgct agagagtatt tatagtgatt tttctattgg aatacgttat ttatttgtta
    34321 ctttaagtat agctgttctt ttgtgcataa agggttatgt tagcaagaag aataaagata
    34381 ttttaacact aatcggtgta aacaatttat ctagtactaa aattatactg tttcctgaat
    34441 ctataaaaga aggatcaagg ttgttgataa tttttataat gcttatactg ttttctgttt
    34501 tatttggggt gatgtttata
//
LOCUS       DACCCR010000027        31982 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCCR010000027 DACCCR010000000
VERSION     DACCCR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.028"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..379
                     /locus_tag="IVK93_001249"
     CDS             <1..379
                     /locus_tag="IVK93_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6386549.1"
                     /translation="EVDPEEKPDVTPEPDTDARDQGIPEKINKKTIQEDGKKESKKSN
                     LAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQLPKSGESQNKVILW
                     SGIILLSIATMLSAKRFKQNRSL"
     gene            complement(505..1584)
                     /locus_tag="IVK93_001250"
     CDS             complement(505..1584)
                     /locus_tag="IVK93_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6386550.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1693..2133)
                     /locus_tag="IVK93_001251"
     CDS             complement(1693..2133)
                     /locus_tag="IVK93_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6386551.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2190..2600)
                     /locus_tag="IVK93_001252"
     CDS             complement(2190..2600)
                     /locus_tag="IVK93_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6386552.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2781..3215)
                     /locus_tag="IVK93_001253"
     CDS             complement(2781..3215)
                     /locus_tag="IVK93_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6386553.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(3315..4163)
                     /locus_tag="IVK93_001254"
     CDS             complement(3315..4163)
                     /locus_tag="IVK93_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6386554.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4552..6318
                     /locus_tag="IVK93_001255"
     CDS             4552..6318
                     /locus_tag="IVK93_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP6386555.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6420..7679
                     /locus_tag="IVK93_001256"
     CDS             6420..7679
                     /locus_tag="IVK93_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6386556.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7826..9127
                     /locus_tag="IVK93_001257"
     CDS             7826..9127
                     /locus_tag="IVK93_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6386557.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9130..9981
                     /locus_tag="IVK93_001258"
     CDS             9130..9981
                     /locus_tag="IVK93_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6386558.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9994..10809
                     /locus_tag="IVK93_001259"
     CDS             9994..10809
                     /locus_tag="IVK93_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP6386559.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10960..12126
                     /locus_tag="IVK93_001260"
     CDS             10960..12126
                     /locus_tag="IVK93_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6386560.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            12356..13003
                     /locus_tag="IVK93_001261"
     CDS             12356..13003
                     /locus_tag="IVK93_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP6386561.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13087..14031
                     /locus_tag="IVK93_001262"
     CDS             13087..14031
                     /locus_tag="IVK93_001262"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP6386562.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14166..15617
                     /locus_tag="IVK93_001263"
     CDS             14166..15617
                     /locus_tag="IVK93_001263"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP6386563.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15638..16474
                     /locus_tag="IVK93_001264"
     CDS             15638..16474
                     /locus_tag="IVK93_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6386564.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            16474..17133
                     /locus_tag="IVK93_001265"
     CDS             16474..17133
                     /locus_tag="IVK93_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6386565.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            17259..17948
                     /locus_tag="IVK93_001266"
     CDS             17259..17948
                     /locus_tag="IVK93_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386566.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            17954..18910
                     /locus_tag="IVK93_001267"
     CDS             17954..18910
                     /locus_tag="IVK93_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP6386567.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            18954..19466
                     /locus_tag="IVK93_001268"
     CDS             18954..19466
                     /locus_tag="IVK93_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6386568.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(19722..20270)
                     /locus_tag="IVK93_001269"
     CDS             complement(19722..20270)
                     /locus_tag="IVK93_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6386569.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(20359..21540)
                     /locus_tag="IVK93_001270"
     CDS             complement(20359..21540)
                     /locus_tag="IVK93_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP6386570.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(21776..23284)
                     /locus_tag="IVK93_001271"
     CDS             complement(21776..23284)
                     /locus_tag="IVK93_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP6386571.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            23443..23634
                     /locus_tag="IVK93_001272"
     CDS             23443..23634
                     /locus_tag="IVK93_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386572.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            23837..24754
                     /locus_tag="IVK93_001273"
     CDS             23837..24754
                     /locus_tag="IVK93_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP6386573.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            24747..25820
                     /locus_tag="IVK93_001274"
     CDS             24747..25820
                     /locus_tag="IVK93_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386574.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            25968..26486
                     /locus_tag="IVK93_001275"
     CDS             25968..26486
                     /locus_tag="IVK93_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386575.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            26467..26859
                     /locus_tag="IVK93_001276"
     CDS             26467..26859
                     /locus_tag="IVK93_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP6386576.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            26935..27870
                     /locus_tag="IVK93_001277"
     CDS             26935..27870
                     /locus_tag="IVK93_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP6386577.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28003..28782
                     /locus_tag="IVK93_001278"
     CDS             28003..28782
                     /locus_tag="IVK93_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP6386578.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            28775..29092
                     /locus_tag="IVK93_001279"
     CDS             28775..29092
                     /locus_tag="IVK93_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6386579.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            29336..30946
                     /locus_tag="IVK93_001280"
     CDS             29336..30946
                     /locus_tag="IVK93_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6386580.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31103..31951
                     /locus_tag="IVK93_001281"
     CDS             31103..31951
                     /locus_tag="IVK93_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6386581.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        10512 a         5693 c         6256 g         9521 t
ORIGIN      
        1 agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga
       61 ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag
      121 taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg
      181 aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc
      241 tagtcctgaa acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat
      301 actgtggagc ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa
      361 acaaaacaga tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag
      421 aaatatccag aaaacgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat
      481 actttatctt tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg
      541 gatctttttc actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa
      601 gccccaaacg ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg
      661 cagttctgtt ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac
      721 aatcaaaatc gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat
      781 gaaagataga ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa
      841 aatcatgatc gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt
      901 gagcaacgtg tttaaaagag cgataacaaa ttgagcttct aataccaaac caaatgtatt
      961 gacaaactta tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa
     1021 aaacaatctt gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat
     1081 gaatgacata gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat
     1141 cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata
     1201 ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa aaatcaataa cggactggat
     1261 catatcaaat gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag
     1321 gtaaacgatc agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg
     1381 ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt
     1441 tgtaatcata cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg
     1501 gcgtattttt tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata
     1561 acaaaaaata aacacgttat tcattatatg atggaatatg aaaataagaa agagaaagag
     1621 caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt
     1681 catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt
     1741 cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg
     1801 tggaatacct tctgcttgtt tttgcaacgc agcaccttga ggagtcagcg taacatttac
     1861 gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt
     1921 tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga
     1981 agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag
     2041 cggcttgagg agggaagtgt agtgtctaat gatttcctta gaggcggcat ataaaggaaa
     2101 acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag
     2161 tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct
     2221 atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct
     2281 tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag
     2341 ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata
     2401 tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca
     2461 aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat
     2521 tcgaatgtac gatcgggatt atgaacttct ccagagcgtc cgcctgtgtt gatcattttc
     2581 gtagaataca tttttttcat aaaattacca cctttaatta tattttatac aattgaattt
     2641 tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc
     2701 acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaaatat cagagacaaa
     2761 aactaatctc atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa
     2821 cttacttcac gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt
     2881 gagttagtca caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt
     2941 cgtaatgctt tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta
     3001 tttggatgtt cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg
     3061 aatctcgtgc catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc
     3121 tcaatcagtt gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca
     3181 tttaatacaa aatctatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg
     3241 aacagttact agattctttt attataccag ttaaagaaaa cgcattcaaa aaaatagcgg
     3301 atttacttac caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct
     3361 acgctgctga caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg
     3421 gaaaattcat gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg
     3481 ccttttgcct ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg
     3541 tcttccaaat aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg
     3601 atataagtca tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct
     3661 attccaaaaa caaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc
     3721 gaagtattca agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta
     3781 gaagagttgt ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg
     3841 acagacaaca accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga
     3901 aaaaacaagc ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct
     3961 agtaccaaca taacgatatt caacactaaa ataactatag ctcggtcaaa tcctaaaaca
     4021 gcttccagta atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa
     4081 aacatattga tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat
     4141 ataaattgtc tcgtttcttt catttatcca cttccttaat tcattaataa tactcttatc
     4201 ttattataac ttacttttca ccttaagtta actttaatta ataaaaaaat taggctagtc
     4261 taaattatta tgtttcattc ttctagaata tacttctacg ttaaaaactg ttaaaaaact
     4321 tttactttag aaaattggag aaattgatcg cttaagataa attcaggaca ctaaatagta
     4381 caggaagtag attaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa
     4441 taaaaattcg gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgtt
     4501 tctctatcac ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca
     4561 gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt
     4621 cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct ttgtggagat
     4681 ccatatacca taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta
     4741 agtaccaatg ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag gcgcttacag
     4801 tatgcgttcc atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt
     4861 tttccttatc gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt
     4921 catcaaatcg atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt
     4981 tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg
     5041 ggaagtaaag atccagaccg agaagacttc ttcgggggag atttgcaagg tgtgagggat
     5101 catttggatt acttgacgga ccttggcgtc aatggcatct atttttgtcc catatttaaa
     5161 gccacatcca atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc
     5221 gataaaaaac ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg
     5281 ttcgatgctg tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa
     5341 aacggagaaa aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct
     5401 tatcagatga ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt
     5461 actccattca tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat
     5521 atcgcaactt attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat
     5581 gaagtcgatc atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac
     5641 atctatatcc ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc
     5701 catgcagtga tgaattatgc attcacagat tcgattaaag attattttgc aaaaaagaaa
     5761 atcacagctt cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag
     5821 gtgaacgaag gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt
     5881 tgccaaggta acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg
     5941 tctccttgta tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc
     6001 cgcaaatgta tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa
     6061 gaacttgttt ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg
     6121 ctaatagtat atgatcgtga agataagctc tatttcacac gggaactaga gggacagatc
     6181 atctatgttt actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg
     6241 attcttagtc aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga
     6301 tttttgatca tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag
     6361 atttgtgccc attataataa gggtgtaaca gcacgttgaa tacacaggag gaaatgaaga
     6421 tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg
     6481 gattagcggc ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa
     6541 cgttaacagt ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt
     6601 tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg
     6661 aatcattagc gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg
     6721 accggatcgg tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag
     6781 cagcttacga tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac
     6841 ctgcagtaat cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat
     6901 caacgtttaa agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag
     6961 caggaaaaaa tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa
     7021 ttgcaggtta cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt
     7081 tgaataatgc aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt
     7141 ggccaaaggg aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt
     7201 ccaataaaac tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca
     7261 atatcaatta tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt
     7321 ttggcggagg aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa
     7381 aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg
     7441 aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa
     7501 cagctgtgat tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg
     7561 aagtttggac aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa
     7621 aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat
     7681 taaaatgata accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca
     7741 agagcgagat ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt
     7801 tagcaagttt agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa
     7861 aaaaatcacg ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt
     7921 taaaggtatc gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg
     7981 atttcatgca ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt
     8041 atttattttg attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta
     8101 cggtttgaat cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat
     8161 caatcggacg atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt
     8221 gacacttccc gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt
     8281 gttcactgca tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg
     8341 ggtaggggct aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt
     8401 tgcagtcttt tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct
     8461 tggtattctc acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg
     8521 tatcattttc ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa
     8581 tatattcaac gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct
     8641 tccttttgta gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc
     8701 aattatctta gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat
     8761 cttacaatcg atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg
     8821 gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat
     8881 tacacagtat acggggaatt tcaataattt ctccatgatc tatctgttta acaatggtgg
     8941 tcccggaagc gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa
     9001 acttacgact ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc
     9061 cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg
     9121 ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt
     9181 tttacttact tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca
     9241 gcaagtactg ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac
     9301 tggactttag gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa
     9361 aatacattga tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg
     9421 ggctatactt atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta
     9481 gtgattcaga tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta
     9541 ttagaagcgc tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca
     9601 atgaatactt ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct
     9661 gctaaacttg atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc
     9721 aaaccgatgc tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg
     9781 ttagctaagt tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca
     9841 tttatttctg atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt
     9901 gcactaccga tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca
     9961 tctggcggaa caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt
    10021 tccgcattac gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa
    10081 tttttcttga tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat
    10141 gccaatcaga tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat
    10201 gaacaaggaa cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg
    10261 caacaaacga tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa
    10321 caatccgatc atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag
    10381 gataaatctg gtatacgccg ctatacgatg aattatagcc catctttcca accagattct
    10441 gtacgtacgc ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt
    10501 ccaacaatca ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt
    10561 ctcattttat tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc
    10621 attcatacat tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg
    10681 gcagcagcag ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca
    10741 acgataactg tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt
    10801 gtcgaatgac aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa
    10861 gtagtttttg aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag
    10921 tattaacgta atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac
    10981 aatcaattat tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca
    11041 acgaaacaat acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa
    11101 atcccaatga aagagattac aagtaacgct tctttggcac taggagaagc ctatatggat
    11161 catcgaatcg agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca
    11221 aacagctttt tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg
    11281 aaacaaaaaa acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa
    11341 ctttggctag atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg
    11401 ttggaacaag cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa
    11461 ggcgaaacgt tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa
    11521 gagtataatg tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc
    11581 aaaagaataa aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc
    11641 gaactaaaag gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt
    11701 tctgaaaatc ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca
    11761 gctttgatcc atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa
    11821 tatattttcc ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa
    11881 aacgatttgc aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa
    11941 cattggacaa agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc
    12001 ttctatcgaa tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat
    12061 atcgatgtga ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt
    12121 gcataattga ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat
    12181 gctccagaag tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt
    12241 ttctttatat tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt
    12301 tttcttcgtt atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa
    12361 aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa
    12421 taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt
    12481 atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga
    12541 agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga
    12601 taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt
    12661 ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa
    12721 tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa
    12781 gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat
    12841 cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg
    12901 tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa
    12961 tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac
    13021 aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg
    13081 atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat
    13141 gcatctggtg tccactgcat gactacccag gaattagatg aactggctca ttcagaagca
    13201 ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc
    13261 tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct
    13321 tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta
    13381 tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa
    13441 aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa
    13501 ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc
    13561 ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag
    13621 atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc
    13681 aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc
    13741 ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat
    13801 acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat
    13861 gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtacaga gcttcataaa
    13921 gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa
    13981 ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac
    14041 gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg
    14101 caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg
    14161 ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc
    14221 aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt
    14281 ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa
    14341 aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat
    14401 ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt
    14461 atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt
    14521 ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc
    14581 ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg
    14641 ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg
    14701 gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag
    14761 gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt
    14821 tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt
    14881 ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc
    14941 actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa
    15001 aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt
    15061 cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca
    15121 tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg
    15181 gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa
    15241 ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta
    15301 ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca
    15361 tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct
    15421 tgaagatcac accttataca cagttggcta atctgactgg acaaccagca atcagcttgc
    15481 ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa
    15541 gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac
    15601 caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc
    15661 tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga
    15721 agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac
    15781 actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt
    15841 ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga
    15901 tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat
    15961 cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag
    16021 tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa
    16081 aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa
    16141 attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg
    16201 aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga
    16261 tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt
    16321 gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga
    16381 agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc
    16441 gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc
    16501 ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc
    16561 ttcgtgtcgt ttcttttagg aaatttggta gggttgcttt tgacagtttt aggtcttttg
    16621 gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc
    16681 gcactagtcc tgctgtttct gttatatttc gggttacctt atcaattaag tgccttaacg
    16741 gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga
    16801 tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc
    16861 tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc
    16921 agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca
    16981 gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat
    17041 attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat
    17101 ttagaaaaac gttctatcca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg
    17161 gcaccatttc tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg
    17221 taaaatgtaa atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg
    17281 aaatagaatc ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag
    17341 tcacgatgcc tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg
    17401 gaagccgaga atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag
    17461 ccaaactgtc atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag
    17521 aagtaaaaac tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg
    17581 aagaggagat gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc
    17641 ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa
    17701 gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa ttgtacaaag
    17761 aattgaaaag acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt
    17821 ttttagagga aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc
    17881 agcatgatta tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat
    17941 tggagtgaaa aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt
    18001 aaaagaagca ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt
    18061 acgtgattcc gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatccaat
    18121 cgctgtgatc caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag
    18181 ttggacagtc aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct
    18241 cagtgaagta ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa
    18301 agatacagag gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac
    18361 tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac
    18421 gagaaagcat gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct
    18481 ttttttccac gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta
    18541 tttcgataaa gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta
    18601 cggcaatata cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc
    18661 attgaagttt cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca
    18721 actcttgatt acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc
    18781 tggactggga gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct
    18841 gctagatcat ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt
    18901 gatccaatga ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata
    18961 ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg
    19021 atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa
    19081 cgattttgaa cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg
    19141 ctggaacagc tgcattagtc tcaggcgtgg atcccgttta cacaaaaata gatggtagtt
    19201 ggaaagaatc aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc
    19261 gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa
    19321 atatccaaga tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct
    19381 attctgctgg attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa
    19441 aagcatatca aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta
    19501 acactactga aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt
    19561 caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat
    19621 tttacgaaat agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt
    19681 cacgatctct cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt
    19741 tttattttat ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg
    19801 atagctttca aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa
    19861 atctgttcag atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc
    19921 gtaataaaga taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt
    19981 gctgtcaaaa cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg
    20041 gaaccatatt tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc
    20101 tgattgaatg tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt
    20161 ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt
    20221 gaagtttttc cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct
    20281 cctttgatga ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta
    20341 actttgcttt tttgtatttt ataccagcaa gccagtcaca atagcactta aaaagctgac
    20401 taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc
    20461 atttaatccc ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac
    20521 gaggaagaca gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa
    20581 ttctgtcata gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc
    20641 cactgcttct ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag
    20701 gatctcttgg aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa
    20761 agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat
    20821 gtattctccc aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc
    20881 ttctttttct tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa
    20941 gttcaaaaca agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac
    21001 gatcgacata gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga
    21061 taggaagggt aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga
    21121 agctactcca ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc
    21181 aacagctttc ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga
    21241 aataaataca attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac
    21301 accggcaaaa acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt
    21361 tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac
    21421 aagctgtaaa acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct
    21481 gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa
    21541 ataatcactc ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat
    21601 ctttagtgaa attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat
    21661 aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac
    21721 gaaactatcg attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt
    21781 ggtaatatag attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt
    21841 tcaatgtttg ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa
    21901 gttcagctag tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata
    21961 aagcgatcaa gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt
    22021 tcaatccttg tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat
    22081 gggctaaatg ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg
    22141 tacgcccggt aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt
    22201 ttgtaggact ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc
    22261 ggtcttcagg acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt
    22321 cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt
    22381 gtttagaggc ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac
    22441 ccatgtcagt aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt
    22501 tgattacagg aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat
    22561 caaagtggaa ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag
    22621 caatgttcag cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt
    22681 aaccagctgc atgtcctttt tggctttcat gatacagttg gttcaagcaa gtagcgagct
    22741 ttccacttcc tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact
    22801 cattttttcc aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga
    22861 tttctgagtg tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg
    22921 tggcaggctg tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac
    22981 gtaattcatc gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga
    23041 tcttattacg ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca
    23101 atttttgcag caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat
    23161 gcttgtcgtc aactaacttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc
    23221 gttccaaaat atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt
    23281 tcactaggtt ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac
    23341 tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata
    23401 gttattcatt ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa
    23461 aagaaaaacg catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt
    23521 gaagatcccg aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact
    23581 cgtgatgagc atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata
    23641 agataaaggt catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa
    23701 aaaggctgta ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat
    23761 aacaattttt tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta
    23821 agaggaggca agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact
    23881 agaggagacg aacgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa
    23941 agaaaaagca agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc
    24001 tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga
    24061 attaaaaaga agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca
    24121 tgatcgtttt ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat
    24181 gagctattac aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag
    24241 tctgccagaa atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc
    24301 ggagtcaacg gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca
    24361 gacagaaatt gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt
    24421 gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga
    24481 gaagaagaag caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc
    24541 tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg
    24601 tgcagccatc gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg
    24661 attcggtcaa gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa
    24721 tcaatctaat gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa
    24781 atgaagaaca aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta
    24841 atcctctgga tgacacagaa gaaatcccta tgctagagga aacaaaaata catgaaaaag
    24901 aaaaagtaac tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg
    24961 aaaatcccgg gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg
    25021 aaacagcgga agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt
    25081 ttccacaatt attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga
    25141 tcggaaagac aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata
    25201 ttggtctgga acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca
    25261 attatacgtt gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc
    25321 aacgatacca agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca
    25381 atgtccaaga tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct
    25441 ctgcagggca gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa
    25501 cacttggttt taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc
    25561 ttcagagcga atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga
    25621 aaaatccatc ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata
    25681 aaggacagtc tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat
    25741 ctggagacta cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat
    25801 taatggtgag aaagtcctga ttggataagt tggattacca gatgtcattc agataattct
    25861 gttccaaagg acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac
    25921 taaatggacg tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata
    25981 aaatagcaca gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat
    26041 ttattccgaa tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct
    26101 taaaagatgc gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg
    26161 ttggaccatg ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa
    26221 tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc
    26281 tggcgggcat ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc
    26341 ctcaaccttg gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca
    26401 atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag
    26461 gaaaaaatga aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg
    26521 acaagttgca gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca
    26581 tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg
    26641 gtcttcaaaa aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa
    26701 gataacggct tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat
    26761 tggatgttta ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat
    26821 gatggacaat cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa
    26881 gggcgaatat tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa
    26941 aattttaaaa aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt
    27001 gctatcagca aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc
    27061 atccacagta tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc
    27121 aataaaggac agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt
    27181 acaatcgtcg caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt
    27241 gaaaagttga cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg
    27301 ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa
    27361 ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata
    27421 ctgattattt ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga
    27481 acaatattga cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc
    27541 ggtttcttgc tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc
    27601 ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag
    27661 atcagtcggc tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca
    27721 gcaaaagcag atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt
    27781 gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat
    27841 aaatatgacc caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg
    27901 agaaaaatat aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa
    27961 tgagtccgct gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt
    28021 gcgccatggc ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta
    28081 ggattagctt gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg
    28141 ttatcgattc tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg
    28201 caagcatcat ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta
    28261 ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt
    28321 tctcaatcac tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct
    28381 tcctggaata aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt
    28441 agcaatatga tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat
    28501 tttgctttga cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg
    28561 atctttacag gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat
    28621 actttcggat tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag
    28681 gcattcaaaa aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg
    28741 ctcttcaact tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt
    28801 agttgcttgt ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac
    28861 tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt
    28921 tgcggcgtta gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa
    28981 tatcaaaata gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc
    29041 gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt
    29101 attttattaa aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat
    29161 ttgcaacagt caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc
    29221 ttatgatgca agctcttttt tattttagga attagagagg gatttgtcct tctatgattt
    29281 tttgctataa tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa
    29341 taaaaaattg atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt
    29401 aggggtagtc tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct
    29461 tgctacgaca ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac
    29521 agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa
    29581 cgctgcttgt cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc
    29641 ctctgctgca ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa
    29701 cgttaataat gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat
    29761 tcttagtgca atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc
    29821 tttgattatc gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg
    29881 cgtattgact gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt
    29941 cggtgggtta gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga
    30001 acttaaagat tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt
    30061 ctctatcatt cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca
    30121 aatgatcgac caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga
    30181 tcagcaactg gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat
    30241 cctgatctcc atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa
    30301 gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt
    30361 gattcttctt ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt
    30421 ttttggctat gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt
    30481 tttctcttta ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc
    30541 gatccgatta actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg
    30601 attagttggc ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt
    30661 tcgcggctat cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa
    30721 ttttttctta aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt
    30781 ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt
    30841 ggctgtagga agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt
    30901 tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt
    30961 ttttgctgtc acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc
    31021 caattttagt ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct
    31081 ttgaaacgag gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct
    31141 gccggaacgt atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga
    31201 tgggaaaatt tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa
    31261 aaacagtaaa gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata
    31321 tgaggaattg aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat
    31381 ttctggcttt gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat
    31441 tccttatgac tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga
    31501 cttaaatgat gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga
    31561 taatacctat gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt
    31621 gacaaatggt gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc
    31681 tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc
    31741 tgaagaaatt attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat
    31801 acatgcaaat aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc
    31861 aaatgcagaa atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa
    31921 agccatcggc attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa
    31981 ta
//
LOCUS       DACCCR010000028        30602 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCCR010000028 DACCCR010000000
VERSION     DACCCR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.029"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(257..598)
                     /locus_tag="IVK93_001282"
     CDS             complement(257..598)
                     /locus_tag="IVK93_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6386582.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(598..828)
                     /locus_tag="IVK93_001283"
     CDS             complement(598..828)
                     /locus_tag="IVK93_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6386583.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1124..1462
                     /locus_tag="IVK93_001284"
     CDS             1124..1462
                     /locus_tag="IVK93_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386584.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPIQIET
                     LVQRISTREDFAIVECEIVAIFVEEGILHDPTHINVDKWQPLIYKLREYTTANQSLSL
                     NFRFQEFKES"
     gene            complement(1667..2242)
                     /locus_tag="IVK93_001285"
     CDS             complement(1667..2242)
                     /locus_tag="IVK93_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP6386585.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDVSFARKIITQQMPELVMEKV"
     gene            complement(2607..3188)
                     /locus_tag="IVK93_001286"
     CDS             complement(2607..3188)
                     /locus_tag="IVK93_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6386586.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASETGKGQFYYYFS
                     SKKEVCLAVIHAHMVVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWIKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3371..3997)
                     /locus_tag="IVK93_001287"
     CDS             complement(3371..3997)
                     /locus_tag="IVK93_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6386587.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFSLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4019..5347)
                     /locus_tag="IVK93_001288"
     CDS             complement(4019..5347)
                     /locus_tag="IVK93_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP6386588.1"
                     /translation="MEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCI
                     NIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFL
                     SNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEK
                     LVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFHL
                     GVSVDQVADQESTAAVTFTENGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDRG
                     AIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVPY
                     SVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDL
                     ILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5506..5907)
                     /locus_tag="IVK93_001289"
     CDS             complement(5506..5907)
                     /locus_tag="IVK93_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6386589.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5993..6199)
                     /locus_tag="IVK93_001290"
     CDS             complement(5993..6199)
                     /locus_tag="IVK93_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386590.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6294..7796)
                     /locus_tag="IVK93_001291"
     CDS             complement(6294..7796)
                     /locus_tag="IVK93_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP6386591.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7814..8269)
                     /gene="argR"
                     /locus_tag="IVK93_001292"
     CDS             complement(7814..8269)
                     /gene="argR"
                     /locus_tag="IVK93_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6386592.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8367..8984)
                     /locus_tag="IVK93_001293"
     CDS             complement(8367..8984)
                     /locus_tag="IVK93_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386593.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9205..10578
                     /locus_tag="IVK93_001294"
     CDS             9205..10578
                     /locus_tag="IVK93_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6386594.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10653..11765
                     /locus_tag="IVK93_001295"
     CDS             10653..11765
                     /locus_tag="IVK93_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP6386595.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11837..13474)
                     /locus_tag="IVK93_001296"
     CDS             complement(11837..13474)
                     /locus_tag="IVK93_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP6386596.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13642..14250)
                     /locus_tag="IVK93_001297"
     CDS             complement(13642..14250)
                     /locus_tag="IVK93_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6386597.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14634..15785)
                     /gene="queG"
                     /locus_tag="IVK93_001298"
     CDS             complement(14634..15785)
                     /gene="queG"
                     /locus_tag="IVK93_001298"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP6386598.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15804..16556)
                     /locus_tag="IVK93_001299"
     CDS             complement(15804..16556)
                     /locus_tag="IVK93_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386599.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16697..17647)
                     /locus_tag="IVK93_001300"
     CDS             complement(16697..17647)
                     /locus_tag="IVK93_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP6386600.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17648..18487)
                     /locus_tag="IVK93_001301"
     CDS             complement(17648..18487)
                     /locus_tag="IVK93_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6386601.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(18484..19230)
                     /locus_tag="IVK93_001302"
     CDS             complement(18484..19230)
                     /locus_tag="IVK93_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6386602.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            19468..20838
                     /locus_tag="IVK93_001303"
     CDS             19468..20838
                     /locus_tag="IVK93_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6386603.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20893..21789)
                     /locus_tag="IVK93_001304"
     CDS             complement(20893..21789)
                     /locus_tag="IVK93_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6386604.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22070..22747)
                     /locus_tag="IVK93_001305"
     CDS             complement(22070..22747)
                     /locus_tag="IVK93_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6386605.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22838..23743)
                     /locus_tag="IVK93_001306"
     CDS             complement(22838..23743)
                     /locus_tag="IVK93_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6386606.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23918..25372
                     /locus_tag="IVK93_001307"
     CDS             23918..25372
                     /locus_tag="IVK93_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6386607.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            25388..26419
                     /locus_tag="IVK93_001308"
     CDS             25388..26419
                     /locus_tag="IVK93_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6386608.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            26426..27613
                     /locus_tag="IVK93_001309"
     CDS             26426..27613
                     /locus_tag="IVK93_001309"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP6386609.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27769..29151
                     /locus_tag="IVK93_001310"
     CDS             27769..29151
                     /locus_tag="IVK93_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP6386610.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            29123..29509
                     /locus_tag="IVK93_001311"
     CDS             29123..29509
                     /locus_tag="IVK93_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386611.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29709..30377)
                     /locus_tag="IVK93_001312"
     CDS             complement(29709..30377)
                     /locus_tag="IVK93_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP6386612.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8610 a         6228 c         5558 g        10206 t
ORIGIN      
        1 tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa
       61 gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat
      121 gctatatatt ctttagttta agatacggaa attaatccca tcgaccacac tagtagaatg
      181 aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa aatattatag
      241 agtttactta caatgcttaa aacatatact gtataatctg atcaatttta gccatgtctt
      301 gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga gattttaatt
      361 gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa cgtgtcggta
      421 aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta cggttaaatt
      481 cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct actgagggat
      541 caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct tctgacacta
      601 taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat aatactcggc
      661 ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg ttccatcatc
      721 agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat cagaaggtaa
      781 agttaccact acagaacttc cttgtaatct agattttgta acaagcatta tcatcactcc
      841 cttaataaaa gtatactata taacactaag taacgcaaaa ttcccctttg taaactatct
      901 agaaaattgt tttgtttaaa gtatatctta ttggatataa atagaaataa attgtagcca
      961 gttcattttt tctttaaata aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa
     1021 gctacccaaa tactatagct aactccccgc taccttcaat cttgcagata aaacgctttg
     1081 gaaaaaaatc gagaaaatcg caaagacgac aggccatcct atcatgcctg attataaaca
     1141 aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca cgaaactagc
     1201 aggtgaagaa gtttcaactg ttcggattaa agaatgcccg attcaaatag aaacgttggt
     1261 tcaacgaatt tcaactagag aagactttgc tattgtagaa tgtgagatcg tagctatttt
     1321 tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat ggcaacctct
     1381 tatctataaa ctcagagaat atacaactgc caaccagtcg ttaagtttga attttagatt
     1441 ccaagaattc aaagagtcat aatgataaac atcattaggt ataaataaaa aagactcgca
     1501 gaacgagtaa aaaacaaata gaagctatac cttagaaagg tcgactgtga ctctatagat
     1561 gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga tctgtgctga
     1621 agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat accttctcca
     1681 ttactaattc aggcatctgt tgagtaatga tttttcgcgc aaaagaaaca tctgttaccc
     1741 aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg
     1801 tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc tgatggctac
     1861 gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat ttgtcttttt
     1921 cttcattcca ctcataaaag ccactcattg ggaaaacaca acgattttgc aaaaatggtt
     1981 gatgaaaggt ctttttttct tctactgtct ctgcacgggc attaatcatc aattgtcctt
     2041 ttttaaatcc ggtaaatccc cacttcatga tttccggagt gaggatgttt tcttcctctt
     2101 ttcttaacgt cacaacaaga tttgacggaa aaacttctcc agctgctaat tctgctgcct
     2161 gctccggttg tttcgtctgg gcttctcgat aataattctg aaggagctct gactctagat
     2221 caaaaaaata acgaccacac atcctaatcc ctcccaatct ttttttctat catacactgc
     2281 aacaagaagt ccgtaccaac aaaacgcttt caatttgtgt agaactaaaa aagttgtgac
     2341 aagtgacaaa ctctccagtg aatggtattc tcctgccaac tcctcggtca caagtcacaa
     2401 tattcaacaa acatgagaag ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg
     2461 gcaggcttac gacctctggg taaacggtgt tccagaagta gcttcttcga aaaataagcc
     2521 gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg
     2581 accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct
     2641 ttgatcaatt gaaaattttg tctaagtagt gatagatcct gtgtgacttt caacaataag
     2701 atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac
     2761 agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgatcca ttgattgact
     2821 aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta
     2881 cctacaggac agccatgata agtgatctgt gtctcatgat cagcaaaaat ccagtccaac
     2941 atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct
     3001 gtctgccaca cgaccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa
     3061 aagtaataat aaaattgccc tttccccgtc tcagaagctg ctagaatatc tcggattgac
     3121 gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt
     3181 gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca ataaattcat
     3241 cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac
     3301 tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc
     3361 aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa
     3421 taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg accaggagca ccaattgcat
     3481 ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac
     3541 acaagtttcc gttcattcca ccaagaatca ctgtgtcgat tccatttttt ctcaattgga
     3601 gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgata atcgtatttt
     3661 catccaaata tggtttcaat tcttcgatga aatcagctcc tgaaccttct tcaactgatt
     3721 catattgact tttcctagca aacatctgtt gctcaagcat catatgttct cctgcaccgc
     3781 caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc
     3841 ctttttcttt tgcttgtttc accatttcga tcatgttttc gatcgtattg tatttttcta
     3901 agatatcttt tgtcaaacta aagccaccgc ctgctggttt caagaactcg acttgcgggt
     3961 cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt
     4021 attttaatac atcatttaaa gcttcactca ttgttggatg agaatagatt tgatctctca
     4081 ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag
     4141 aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt
     4201 tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact
     4261 tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg
     4321 ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt
     4381 tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag atataagtga
     4441 actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt
     4501 attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa
     4561 gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta
     4621 cttcttggcc attttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat
     4681 ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt
     4741 gcgcgatatc ttcatcttca cgaggaagaa attcaggatg ggcatcaaga acgacaactt
     4801 tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga
     4861 tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc
     4921 gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa
     4981 tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag
     5041 aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat
     5101 aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt
     5161 cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac
     5221 atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattcg ccttttgaag
     5281 ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt
     5341 tttccatcct ttttccctcg cttctttttt tacctaccga ttggtacaaa aatatgataa
     5401 ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac cagacaaaaa
     5461 gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa caatcgttac
     5521 ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat gcgcatcttc
     5581 tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta atactacacc
     5641 gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta ctgtggactc
     5701 tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac aagaagcata
     5761 acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg gaggagtgat
     5821 tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc catggttgat
     5881 cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc atttgctact
     5941 tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct gcttattcga
     6001 ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta ctcgtgaaca
     6061 acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct tttatatgat
     6121 tgattttaag atacatatat accagaaagt tgaccccata aaccaatact gttaaaaaaa
     6181 taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag cactcgattt
     6241 aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact ggtttattct
     6301 gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt tactaacgcc
     6361 aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg ctcatgtaag
     6421 cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata tcgaatacct
     6481 tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg cttcatttct
     6541 tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa tcggatttct
     6601 tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat accttttctt
     6661 tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc atccagaaaa
     6721 tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt atctttccca
     6781 agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat ataacgaact
     6841 tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg ggaattcgtc
     6901 acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc gatcaaatta
     6961 tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac atctgtatcg
     7021 aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact ttccatcgct
     7081 tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc caaaaattgg
     7141 gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa tccaccagca
     7201 ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc tagtgttcca
     7261 atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag gtctgctttt
     7321 tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat aaaagcaata
     7381 tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata ataataggga
     7441 agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa ttgagtggac
     7501 ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc ttgttctttc
     7561 agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc atgaccaatc
     7621 ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat ccagtttgct
     7681 agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata tccttcaact
     7741 ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc tttcatataa
     7801 aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc tgtgcttctt
     7861 cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg acaacttcag
     7921 ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt tcagtcaata
     7981 cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt gctgtacgca
     8041 aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt actattttgt
     8101 tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc gttgcttgag
     8161 tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc ccaataggtt
     8221 gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata ttagacacct
     8281 ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt attttttact
     8341 tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg caaaatcgtc
     8401 ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt gtgttcttta
     8461 gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa cgttgctggt
     8521 tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc catctttttg
     8581 aatactactt gttctttcaa gtcttccgga agtttgctag aacgctgaat ggcttcttgt
     8641 gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga aaaatgttct
     8701 tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc ttccagtgga
     8761 tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga catgcgtatt
     8821 gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg attcgggtcg
     8881 ccgatccctc tgatgctaaa gaatgatttt ttaggaacct ctagtatttg cgggcttttt
     8941 ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct tcctccgcga
     9001 aattccattt agttatttat tatcatacct ctttttgagt gacttggcta atcgtgttga
     9061 cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag ataacacgtt
     9121 tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc tcttttttta
     9181 cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt agtacctgca
     9241 gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc catgaatgta
     9301 actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca atgggtaaca
     9361 acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt gaatcgcaat
     9421 tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg tataatgatc
     9481 gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca aggtgcagcg
     9541 acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc cctcaatcga
     9601 cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc aattggcccc
     9661 acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct gtttcttgtg
     9721 cccgttttga ttctttcttt gattatcggc tggtttgcta ttcccgagat ggcagtacaa
     9781 aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt tagtggaagt
     9841 ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat catcggcggt
     9901 attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccact gatccgactg
     9961 tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt ttatcagttt
    10021 ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca aggaagtagt
    10081 ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt gctttctcct
    10141 ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc tgggctgatt
    10201 ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc agtgggtgta
    10261 ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag caatatgatg
    10321 accaccggaa tgaatgcact gaatgaagaa ctacaaggag acgggaacgc aatgttcaat
    10381 accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat tatcaattac
    10441 gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc aaaagcagca
    10501 ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta ttatctgttc
    10561 tttgctaaaa aagcgtagat tccatatacg gcaggtgttg catctgccgt ttttttgtgt
    10621 gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt atcggtggcg
    10681 ggatcattgg tatgacccta gccagttact tagatacatc aaaattcaat gttgctttat
    10741 ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc ccttggttgt
    10801 cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct tttttcccta
    10861 aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg acacttttgc
    10921 ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga aaagaagaag
    10981 cgccagaaat tggaaacatc gaatggcttt cacctgaaca gacatctcaa gcacttccgt
    11041 tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat ggaaaagcat
    11101 atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc caggcaaaag
    11161 tcactttgca taagaagagc gattcttggc tcatcaaggg accgtttgga gaatacacag
    11221 ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa gaattaggct
    11281 atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca tcttataaga
    11341 tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt cctttcatgg
    11401 atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat ttggctccta
    11461 ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact gatccagatc
    11521 agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg tcagattttt
    11581 ccccattttt cggcgtactg ccagatgatc caagcttact ggctgctagc ggtctaggct
    11641 cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc ttaaatactg
    11701 gtcattggcc tacggaagat tatcaaaagg acatctccac ctatattacg aaaaatccat
    11761 catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg gattacttct
    11821 tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt tcttttgctt
    11881 catctggcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga atatcaagtc
    11941 gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa tctggtatag
    12001 gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt tgttgcacga
    12061 ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg aaatatccag
    12121 tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa gcttgatagt
    12181 cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact agctcatcta
    12241 gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat gttgcaccaa
    12301 aagacgctgc taaaactggg tcattgatct tgataattgg cggaagcgaa tcatctttca
    12361 tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg ggtgtcgcat
    12421 aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact aggacttttt
    12481 ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa gaaatggccg
    12541 gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag gcagtagcaa
    12601 aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca gagtgacctt
    12661 ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca aatacagcca
    12721 tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag gcaataaagc
    12781 cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat tctccaaagt
    12841 aacgtaatcc tagcacagcc gctgtattat ggagagcatc aaacaaaatg agtccatttg
    12901 gaaatgtttc atgtgtccat tcgggatcac aaaagatata aagatctccc tcatcaaagg
    12961 aaacagactt ttgataagtc tctaagatct tcgaattcgc tggctgaaaa ttcagcaaat
    13021 aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc acagaaaatt
    13081 cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgcttgcga ctttgataca
    13141 cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc tcaaagatcc
    13201 gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt gtcctaacat
    13261 cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg atcgttcctg
    13321 gactcccttt tgctagttga taagctgtct tcctatcaaa aacttcatga acattattac
    13381 cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt ccacttgtga
    13441 tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc ctaaacaaaa
    13501 aaacgttttc tttattatac attattcgag tttttatttg tactgctgcg ctgtgggaag
    13561 caaacgagtt atttttcaca caaaaaaacc gagaacttga ataaacaagt ttctcggttt
    13621 catcattttc cttttatcgg cttagtacca gccgtttgct aaccagaatg ttttagcagc
    13681 atcccatgaa ccataacgag atgttacata ttgttctgct acacgttctt gattggcagc
    13741 tgaatagtcg ccgtttaagt atgaagcatc taattgataa cggccgatgt aacgtccgtt
    13801 agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg cagagcttga
    13861 agttggcgct gttgcagcag ctggggcagc aggagctact tcaactgttt caacaggtgc
    13921 aacgtatgta tgttcttgcg ttgctgtttc agttgtagat gctgtatttt ctgttgtttt
    13981 gttttctgtt gttgctgaag aatcatttga agttgggatt gttaattgtt caccaacata
    14041 aatgcggttg atgtttgcga tcgaattttt ttcagcaatt gcatcgatca ttgagttgtc
    14101 tcctgcaaat ttttgtgaga tcttagataa tgaatcacct gattttactg tataaacttc
    14161 atcagcgtga gcatttgtag ttcctacaaa cattgcagta gcaccagcag caaaagttgt
    14221 acctaaaaca attttcttaa atgaattcat attaaactcc tttttttctc cgttttttat
    14281 tctttcgacg ctattaactt tatcatttag aaatatttca taggtaaaag aaacatgaca
    14341 tttaatgaca aaataacgaa ttatttaata tttgtaacat ttaaacaaat atttttttga
    14401 tacaaaacca taaaagcctt gttgtataag caaagtaatc gtttttctat gtttttttat
    14461 ttttgaatga aaaacgcaga ttctgtaaca ttcactgcaa taatccataa aaaaaagcaa
    14521 aaaaattttt gatttttatc aaattctctt aaaaaaatgt aaaaaataca aaaaaaccag
    14581 taattgttac aacaattact ggacaaaaac tttgaataat gttaactatt tttttatttt
    14641 aactttctcc gcaataattt ctgggcatgt tccaattcgt ttataacgac ttcttctgtt
    14701 tctttagcta atatttcatc taatacatca agccattttt ccggttcttt tgttcctaga
    14761 cgtccaatag cccaagctgc tgttcctctg atgacagggc ggacatctgt taaacattct
    14821 gaaatctcag gcaatgccgt tcgatcacct agattagcta aagcaatcaa tgcgtttctc
    14881 tgcaaaggtt tcttcccacg ccatgaacca gctaaatgcc caaattgctg tttgaagtct
    14941 ttattagaca tcgaaagcat tggctttaat tttggatata tttcgtctac ttttggttcc
    15001 atctcttcat gaaaatgaaa atcttttcct tgattgtaag ggcagactaa ctggcagata
    15061 tcacatccat aaatgacatt tcgcattttt ttacgatatt cttccggcat catccctttt
    15121 gtctgagttt gataagacaa acatcgctgg gcattcattc ttccgtcacc aagcaatgct
    15181 tttgtcggac aagcagtcac gcagcgcata cagtcaccgc aaccaaattc tcctggttta
    15241 tcaggctcaa atacaatatt agtaacgatc tcaccaagat agacaaatga accgaactct
    15301 tcagtgatca acaatccgtt tcgtccaata aatcctaaac cagctcgctg tgctacagcg
    15361 acatcgatca gttctccagt atctacttgc ggagctagac gccattcctc ttctttttgc
    15421 catttttctg ctcttgtttg gatgaattcg atcaatcggt tcaaacggtc tctgagtata
    15481 tcatggtaat cgatccccca agaagcacga gcaaactgcc cgcgtttttc atccctaggc
    15541 acttcttcat ggatcctagt aggatacgca agtgcaatgg aaatgatcga tttcggctga
    15601 tcaaatgtca tttctggata aagacgctca tcgatatttg gatgttcaaa gccagaagta
    15661 tggccagctg ctttttgttc ttccaacgat tcttttaatg aatcaaaagg ttccgccgtg
    15721 gtgaacccga ttttgtcaat tcctaatcgt ttgctttctt gtatgatttc ttcttttaat
    15781 gtcatggtct tcctcgcttg ctgttaacgg ctgtataacc cataatatag tttttctttt
    15841 ttcagctgga caaactggat ctgctgatcg gcaatcttct tttcttccat ccattttttt
    15901 cttgcagcaa atacttccca ataatttgtt gaaattaatt ttcttttgtt atctaatgca
    15961 ttaaaaaaat tcaatgaaac tggttttgta cttaacagtt catggagcct tacctcttcc
    16021 catggcgaaa actcagacag atcgcgggaa tacaatactt cttcatagag atttttttgc
    16081 tgtgtgtacg tgaatgtttg cgggattttc ccaggcctag caatttctac tagacgatta
    16141 taaaacatat tcattaattt gctgttggca ataaattgac ttttataata ggccgactgg
    16201 atctttttga ttactccttc tgctttgatg actgaaaata atgcaggcca tgctttgacg
    16261 gttatttcgt cgttttgttt aactaatacg ccaggtgtga agccgactgg agaaaggaac
    16321 acaaaataac cgataccggt attttcatct aaaggctgaa attcatcaaa caaccatttg
    16381 cctatgtaca gctcttcatc tggttctgtt gtctcatttt gatttgctat gaataattta
    16441 gctaattctg cttcaagaaa ttgaaggtct tgatccttgt cggtattttc ctgctggctg
    16501 atcaatcgaa agacttcttc tttaatcgta tatttatctt tacgtgtaaa attcatagcc
    16561 acaccttcct tccttactct aactataacg gaatttcttc aaaaaaggta tctgtacgac
    16621 gaaaaacagc ataatttttt ttcatatctc ttataaaaaa tgcaaggtct gctcctcaag
    16681 ctagaccttg cacttttcag ctttcttttg tttgtgacaa accttcatgg atcgtttcaa
    16741 tattccattt catcatctga tagtaagaat ctccttgttc tccttctttt gctatggaat
    16801 cagtgaacag tttatcatag attttcaaac ccgtttcttt tgctacccgt tccatgcttc
    16861 ttgagtctac actggtttcc acgaataaaa ccggtacttc ttttgctctt atctgatcaa
    16921 tgatcgcttt catttgatca ggcgtacctt gactttctgt attgatctcc catatataag
    16981 ctgctggcaa atcatatgct cgtgaaaaat acttgaatgc cccttcactt gttactaata
    17041 attttttgtt ctcttcgata gaagcaaaac tttccttcgc ttgtgtatct aattctttta
    17101 gtttttccac atatgcttct gcattttttt tatagagtgt cgcattttct gcatcttttt
    17161 cagagaatat acgtgcgatt tcctccacat attttattcc gttagatagg tctagccaag
    17221 catggggatc tgcttttgta tgttcttcac cgctagttaa ataaagaggt tctacatttt
    17281 tgctaactgc aaaataatct tttccttctt cttttttagc cgtttccatc aagttatcga
    17341 accagctgtt acctgtttca agattcaaac cgttgtataa aataacatct gcatcgcttg
    17401 cctttttgat gtcttctggt agtacttcat attcatgcgg atctgttccg actggtacga
    17461 tactgtggat atctatttga tctgttccga cctcttttgc catgtccgcc aagatcgaat
    17521 tggtagccac gactgacaac ttttctttac ttccttcatc cgcttggtca gaagcctgct
    17581 ttccgcaacc ggctaatacc aagaaactaa ctgccaagat taaaaagagt gatttttttg
    17641 ttttcatcta tctgcttcct ttctgaaata aaattccttt tgttggagaa aataaaaaag
    17701 caaggataaa aaatgcggta catgctaaca cgatcgtagc tcctgatggc caattgtaac
    17761 tataactgaa aaacacaccg acaactgcac ttagcatacc gattcctgca gctgtaatga
    17821 tcattttcag taaatggttc gttagcaagt aagccgtggc ggctggtgtg atcaacatgg
    17881 caatcaccaa gattgttcct actgtctgta aactgactac agccactaat gtcaaaaaga
    17941 acatcaaaag ataatggatc agcgatgtgt tcagcccata agcttttgcc attgtcggat
    18001 cgaatgatgt gatcttcaat tctttataaa agaaaaagac aaatatcaag acgattccac
    18061 taacgagtgc tgtcagcaac aaatcgcttt ccctaacagc taagacattc ccaaataaaa
    18121 tatgatacaa atctgtcgca ctctgggcat atgaaatcaa gataatccct aatgcaaaaa
    18181 aggagctaaa gacaattcca atcgctgtat catttttcaa tcggctccgc tgagtcacga
    18241 aaccaatcac tgctgctgtt agcatcccaa aagcagtggc tccaaagatg taactaaaac
    18301 caaacatata agaaatggct acacctggaa gaactgcgtg agagatagcg tctcccatca
    18361 aagacatccc tcgtaaaata ataaaagaac cgatgactcc agaagcaagt cctacaagca
    18421 tggaagtgac caaagcgtat tgcaggaatt gataattcat taatccatca ataaaggatt
    18481 ggatcatttt ttctctcctc cttttactaa atgtctgatg atctcaccat aggccaattg
    18541 aaggttcttc gatgtaaaga cttcttgtac tggaccggaa gcgatcagct gtttatttaa
    18601 aagaataacc tcatcgaaat attcttctac tttatgaaga tcatgatgga cgatcaaaat
    18661 cgtttttcct gaattcttca attcctgcaa gatgtcaaag atctttcgtt cacttaacgc
    18721 atcaatccct acgaatggtt catctaaaaa gatccattct gctccttggg ctagagctct
    18781 tgcaataaaa actctctgaa gctgtccacc cgatagttcg ctgatctgtc ttttggcata
    18841 ttcttccatc cctacttttt tcaaagcttg tcttgcacgt tctttttctt ttttcccagg
    18901 tctttgtcca attcgtaaag atggatatgt tcctaaaagt acaacgccta atacatcaat
    18961 tggaaaggaa agatccaatt cacttcgctg ttccacgtag gctatttttt tacggactga
    19021 gtttaccggc tgtccagaaa aggtgacttt accagtagta gctggtatca actctagcaa
    19081 agatttcata aatgttgatt ttcctgcacc gtttggtccg ataataccgg taattttttg
    19141 tggatttatc gtcaagttga tagttttcaa aacagttttc ccttgataag cggctgaaag
    19201 gtcttgaatt acaatggccg tacgttccaa atcttacact cctttttagg tttacctaac
    19261 tttttattta aatatagttt aactaaaatg gaaagcattt tcaagtagaa tgatgaaaaa
    19321 gtttttttcg aattttcctc gcattttatg aaaattttct gtagaatcac atcaagagtt
    19381 caataaggct aacctaataa tgaaaaacat gtgttgaaac gtttgattat tttttcttgt
    19441 tgactcaaca acgaaaggag tttttgtatg gaagaagaaa agttaactcg aagtcttagt
    19501 gcccgtcata tccaaatgat cgcactaggt ggaacgatcg gtgtcggtct gtttatgggt
    19561 gcctcctcaa ccatacgttg gacagggccc tcagtgatgg gagcctatgc gattgcagga
    19621 ttctttttgt atttgattat gcgtgcttta ggcgaaatgc tttacgttga tccttcgact
    19681 ggttcttttg caaattacgc gagcgagtac atccatcctg tggctggcta cttgaccgct
    19741 tggagtaata tttttcaata tattgtcgta ggaataagtg aagtgattgc tgtaggatct
    19801 tatatgaatt attggtggcc tgatttgccg gcaattattc ccggaatcat tgtcgtcttg
    19861 tttcttatgc tggcaaatct tatttctgtc aaggcgtttg gcgaattaga attttggttt
    19921 tcgatcatca aagtcataac gatcattttg atgatcattg caggtcttgg ggtaatctta
    19981 tttggatttg gtaatcatgg tcaggcagta gggatttcaa atctttggaa aaatggtgga
    20041 ttcttcactg gaggcgtcaa aggatttttc tttgcattat cgatcgttgt tgcttcctat
    20101 caaggaatcg aactgatcgg aatgactgct ggtgaagcag agaatcctaa aaagacgatc
    20161 attgaagctg tccagtccac tatcggtcga atcttgattt tttatattgg tgctattttt
    20221 atcattgtga gtatctatcc gtggaatcaa ttgagtgaga ttggttcacc ttttgtacaa
    20281 actttttcta aagtcggcat tacatttgct gcaggactga ttaattttgt cgtgattaca
    20341 gcggcattat caggttgcaa ttcagggatt ttcagcgcaa gtcgtatggt ctatacactt
    20401 gccataaatg gaaagatgtc taaaaaattt ctgaaactat ctcgtcacgg tgcgcctttt
    20461 tatcctgtcg tagcaatctc aggtggaatc ttaattggtc tgatcttaaa ttatcttcta
    20521 ccaatgttca tcaaccagtc agaaaatatt ttcgttttcg tatacagctc aagtattttg
    20581 cctggtatgg ttccttgggt agtgattttg atcagcgaga ttcgcttcaa aaagaagcac
    20641 gcagataaaa tgcatgatca tccttttaaa atgccactat ctccttatac gaattatgtg
    20701 acacttgctt ttttagctat cgtattgatt ttcatgttta tcaatcctga aacgagaatc
    20761 tcattgatta ttggactcgt tttcttgctt tacatgacga tccattactt tgtacgcgaa
    20821 aaaagaaccg gaaaataaaa aaagaacgaa gcttcacctt agtggaagct tcgttctttt
    20881 ttattgcttt tatcaattca attttagcgt ctcaccggga gtcaggaaat aattctccat
    20941 gctcatccca ttcagcgaca agatcgtttc tactggtacg ccagttcgtt cggctatttg
    21001 atttggacct tctccttcca aaactacggt tgtcgcagcc tgtgaagtcg cgctgttggt
    21061 attagtttca gaggaatctg ttgtgtcttg tgttgttcga gcttgcattt cagaagcttc
    21121 taccgactct gctgtctcag aatcagaaga tgttgtgtct tgtgtgcttg ttgactctgc
    21181 ttgaagagag gcgctagtct gcaaataaat atcggaaatc ttagcgacat gattcgtata
    21241 attagccgat aattgattga tcaaagtatc taagtctaca ccgtctttat tttctgctgc
    21301 ctgatacgct gcacgtaatt ttctgatccc ttcgtcggag attgcttgaa gctcacgtgc
    21361 tttttggtta gcaactgctg ataaaccagc tattccctct tgatttccct gaatttctgt
    21421 ctggtattct tcaatcaatg caggcgtctt ttcttcgatc tttttcgaat aatcatttac
    21481 gattgcttga agttgctctg gcaaaccatt tgctgttttt tcttcagaag tttgtgtctc
    21541 ttttgtttta gttgatgact cacttgtttg tttcgtctct gttttctttg tcgttgtttc
    21601 cgttatggtc gtacttttcg cttctgacga attttttcca tgaaaatata gacccgcaac
    21661 tcctcctgct atgatcaaaa acacggcaac tgctgcccca atcatgatca tccgcttacg
    21721 agctgcctgt ttttgctgtc ttcttcgggt catccgaaca ttttctcccc actcattttc
    21781 atcatacatt tttttcacca ccaattttta tgtataatag cgttatcaaa cgcttcatat
    21841 tcttattgta gcaaataatt gtcgctgtgc acatttttat ctttgttatt tataagaaaa
    21901 tttcaccagt tgagatgatt ccaagattgt ccgtcgattt ctccttgcag ctgcattgtt
    21961 cgttcgtcta tctcagccat cagtttcttt atttcatata agagcgcacg gtcttgatat
    22021 tctgattttt cgcttaattc atctaattgt tggatcagcc aatcatttgt cataatgaag
    22081 ttcctttcat ttcttgcagt gtaaaggtca agtcttcgat ctgctctttt gcctcagtca
    22141 gtgcttctac gatatatgct tgtttttcag attcttcttc ctgcattttc tgattttcct
    22201 gtactctttg aataaaccag tcaatccgct gagtcagcga tggtttgcct tccaatgaga
    22261 gatactgctg ataagcaatt agcttctccc aacgtttttc ctgggataaa tacataaatt
    22321 gtgctaaaac gaatggcgga agataggtca tatggaattt atttgcgatt cgctgatacg
    22381 gcctcaaaaa ttcggaaatc gtatattctt cttttcctcc agcttggtat tctttttccg
    22441 ccacacctgt tgtcaatacg attcctagtt ctttcccttt taatacatgt gctgctcgtt
    22501 ccaagacatt atcttgccaa atttttaaat gagcaggaga actataccag taaaacggaa
    22561 actggaaaat gatccgatca tgctgggcca agagctgtag ttcagtctct atatctattt
    22621 ttccgtctgg ataacttgat tccaaatgat gccaagtagc tggtaaacca gcagaagctt
    22681 ctttgaaaaa ttgctgattt aaagattctt cgatcgttgg atggctaatg atgataagtg
    22741 tacgcatccc ttttcctcct ttttagtctt cgctttacag tgtagcataa ccgccacaag
    22801 tttcttttgc cgatgcttgt cattttaacc agactaatca ggttgtccaa gaaactgcgg
    22861 tttgtttttc caactgtttt ttcaaggtat ccaaagcaat attcttaaaa ttttcgtttt
    22921 tcatacgttt acgatagttc gtagccgttt ctccggtact ctttttgaac attcggctaa
    22981 atacgactag actattgaac cctacttgat aggcaatact ttccacggat tcatccgtca
    23041 gagaaagaag aaacgcccct cgattgatcc gtaccccgat caaatattcc ttgatactgg
    23101 tgcccatatg atttttgaat actcgcgaca aatggctccg attgaccgct aaatgctggg
    23161 cgatttcctc aacagacaat ttttcagcat aatgtgcctc gatgtatttg actgtttcaa
    23221 cagctaaaga ggaatgaacc ttttgtacac ctccgctgac ctttccgcca tcttttaaaa
    23281 ggactgataa aaaacggtag gttaatgctg ttaactgtaa ttcattttcc aaggtttctt
    23341 ccgtatcttt catgcattct aaaataatgt cgatatattt ttgtatgtca gaagagacca
    23401 tcgtgtattg gtcttctctg aaaagcgact gccgaatgat ttcttctgca gcactcccac
    23461 caaatgaaag ccagcaatac atccacggat cttctccatc tgataagtaa aacgtcgagt
    23521 ctcctggacg aatcaagaac agatcacctt ttttcaattg gaattgttga tttttcacat
    23581 gataagatcc tttgccttcc atcacgacat gcaggatgta atcattgcga atagcaggac
    23641 caaaagaatg aaacggcatc gttttagaga aaccacaaaa atcaaaatac aattcttttt
    23701 ctacttgttg atgttccttt tttaaaaaaa tcgattgctc cacgtctatc cctccttact
    23761 cctttagtat aatgcaacaa aaagttaaat aaaagtcaca gaaatatatt aaaaaggaag
    23821 cgatttcttg ttaagtttaa gtcattaaaa gatctttgtt attcgttgta accggtttac
    23881 ttagtattat atattgaaca ggagaggaat acagattatg aaaagcaaaa tgatccaacg
    23941 tgtgtcctac gctttaggcg cttttggaca cgacacattc tatgttacat taagcactta
    24001 ttttatggta ttcgtgacaa gccagatgtt cgcaggaaca gataaggcaa cgaatgcaaa
    24061 aatgattgct acagttacaa gcttagttgt aggtatccgc ttagttgaaa ttatttttga
    24121 tcctttgatt ggtggcatca tcgataatac tcgtacaaaa tacggtaaat tcaaaccatg
    24181 gttagttatc ggcggcgttg ttagttccgt actcttggta gcaatcttta ccaatttctt
    24241 tggcttagct actcataata cgactttatt tatcatatta tttgcaatcg ttttcatctt
    24301 attggatagt ttttattcat ttaaggatat cgctttttgg tcaatgattc cagctttgag
    24361 tacggaaaca aaggaacgtg aaaaatttgc tacgtttgct cgtttcggct cttctctagg
    24421 tgcaaatggt actactctag tcgttgtccc tatcgttact ttctttacct atcttgttac
    24481 tcataaacat attgaaggcg cttcaggctg gttctggttc ggtgtgattg ctgctgttat
    24541 ttcaggcggt actgcgatta tcactgcctt gggcacaaaa gaagtcgata ctgcgatccg
    24601 tcagcaaaat gaaaaagctg ggatcaaaga tgtgttcaaa gcagttgctc aaaatgatca
    24661 gttgatgtgg cttgcccttg cttatatcgc ttatgcgatt gccaatgcag caacaactgc
    24721 cgtattattc tacttcttca aatatgtact tggccaacca actgaatttt ggatcgttgg
    24781 tctagttgct gccttacttg gtttagtagc tgttccacta ttcccaatgc ttacacgcgt
    24841 catctctcgt cgctacgttt accttggagg gatcgcaatg atgatttctt cttacttttt
    24901 ctttacgatc gcaggttcaa gtttgccaat cgttatcatc gggctagtat tgttctactt
    24961 ccctcaacaa ttgattttct tatccgcttt gatgacgatc acagactctg ttgaatatgg
    25021 tcaatggaaa aatggtatcc gtaacgaagc tgttactcta tcaattcgcc ctttactaga
    25081 taaaatcgct ggtgctttgt ctaacgggat cgtagggttt gtcgctatcg cttctgggat
    25141 gactggtaat gctactgctg ctgatatcac tgctcatggc gtaacgactt tcaaagctta
    25201 tgcattctat gctcctgctg ctttgatgat tttctccgct attatttttg cttggaagat
    25261 tacattgaca gagaaaaaac atgcacaaat cgttgagaaa ttggaaaaca aattggctcc
    25321 agctgataca atggaagatg aacttcacga tgcagtagat ccttcccatt aagaaaggat
    25381 cttgaaaatg aagattacag aaaaagattt tggccaaggt tatcacttga taaccttgga
    25441 gaacaaaaac aagctagcac tttctatctc tgatttaggt gctagaatcg tcagtttaaa
    25501 atcaaatgat cgagaattag ttttgggatt tgacaccgct gaagaatata tcgaaaaaga
    25561 tccgtatatc ggtgcttcta tcggtcgcac cgcagggaga atcgaaaatg gccgcttttc
    25621 tctcaatgga aaaacttacc aattagccac agatccaaaa actggacata atctgcatgg
    25681 tggtgctcct ggatttgaat taaaaaaatg gtcttatgtc attttgaatg gcgaaaatga
    25741 agcttctgtc atttttacta ctacaagtcc cgacggagaa catggttttc caggtacaat
    25801 ggatgtggaa atccgctata ctttgacaaa agataatatc tggcgagtga cctctcgcgg
    25861 caccagtgac caagatactt tattcaatcc gacaaatcat gtttacttca acttgacggg
    25921 agatgcttct cagtcaatcg atcaacatga gctttggctc aatagcgaag cttatgctcc
    25981 tttgcgaaca gatagtattc ctataggggt aaaagaaaat gctgcaggaa gtgctttcga
    26041 ttttcaaata cctaaaaaat tagcgtccgt ttttgctagt gatttggatc aaaaaaatct
    26101 tgttgacgga atcgatcatc catttttctt aaaagaaacc ggcttgggta aagaagccgc
    26161 tcgcttgact agccccgata aaagaatcca agtagatatt gctacagatg cttctagtgt
    26221 ggttattttc actgcaaatt tcggcacaga aacaccagaa atgcgcaacc gaaaactggc
    26281 acatcacggt ggcatcacat ttgagactca aacagctcca ggtgcagagc gcttctcagc
    26341 atttggttcg attcatttaa aagccggatc agtatttgaa acagtgacag aattcaaaat
    26401 caaaacaaga aaggaataac tcatcatgga aatcaaaaga agcttaaaca caaaatttgc
    26461 cgaaacattc ggacctaaaa aaactacaca atacttctca ccaggacgca tcaatttgat
    26521 tggtgaacac acagattaca atggaggcca tgtcttcccc gcttccatca cttatggaac
    26581 gcaaggtgta gcagcacctc gagacgataa taaaatatta gtttattcca caaactttga
    26641 cgatatcggt gtcattagtt ttactttaga tgagctagaa tatgataaaa aagcaaactg
    26701 ggcaaattac gtaaaaggaa tggtccttaa attaaaagaa gctggacact caatcgatca
    26761 tgggtttgag ttattagtgg aaggaacaat ccctaatggt gcaggacttt ccagtagcgc
    26821 ttcattagaa ttattagtag gtgttgtatt agaagatctg tttgatttaa aaacagatcg
    26881 cttggaacta gtccaaacag gtaaaaaagt agaaaatgaa ttcatcggtg tcaattcagg
    26941 tatcatggat cagtttgcca tcggcttcgg tgagctagat catgcaatct tattagatac
    27001 aaatacgctg aaatacgaaa tggttccagt gaaactagat ggttatgcaa tcgtcatcat
    27061 gaatacgaat aagcggcggg aattggcaga ttcaaaatac aatgaacgcc gtagcgaatg
    27121 cgaagaagcc ttggcacgtt tgcaaaaaac tttgcctatc tcttctttag gagacttaga
    27181 tgaagaagaa tttgaatcaa ctattgacca aatcggcgat gatacattga tacgcagagc
    27241 tcgccatgcc gtttctgaaa atcagcggac tttgaaggca aaagctgaat tagaagctgg
    27301 gaacttagaa gcatttggtc aactgttaaa tgattctcac cactctcttc gctatgatta
    27361 tgaagtaact gggatcgaat tagatacact agtagatgct gctcaaaaac aagaaggtgt
    27421 cttaggtgca cgaatgacag gagctggatt tggtggctgt gcgattgctc ttgtcaaaga
    27481 agaaaatata cctgccttca aaaacaatgt ctatgatgaa tacatgaaag ttgtcggcta
    27541 tgctccggaa ttttatgttg cacacattgg aaatgggaca acaaagatcg atccagaagc
    27601 tgttcatgtt tgataaaaaa atcaacgatc tcagctaaaa acaaatagat aaaaataacc
    27661 tctcagcaat ttccaaatgt gagccttttg ctcgtatttg aaaattgctg ttttttattg
    27721 acacggaaag agaacgtgtg ttctaattgt gttgaagagg tgattgttat gtgggcgaat
    27781 caaaatccgg tatttgttta tgaaaaagaa ccttcaagag atattttatg tattgactgc
    27841 aaatcttttt acgcctccgt agagtgcgtt gaaaaggatc ttgatcccct tacaactaaa
    27901 ttagtagtca tgtcctatcc gtccgattcg acagaaacac ggggtagcgg attaatcttg
    27961 gcttccagtc ctgcagcaaa aaaagcttac ggcatcacca atatcagccg tgccagagat
    28021 cttcccttcc cctatccaag tgatttatat attgttgccc caaggatggc ctattatatg
    28081 gaaaagaaca gagaaatcaa ttctatctat aaaaaatatg cagacgaagc gaaccatcat
    28141 gtctattccg tcgatgaaag cttccttgat gtaacaaata gcatggcact ctttggtaaa
    28201 acaaatgcgt acgacatggc taaactgatc cataaagatg tgtttgagca gaccggaatc
    28261 tacacgacta taggaattgg cgataacccg ctacttgcta aactagcctt agacaatggc
    28321 tcaaaagacg gaaagcaaat gtttgctgaa tggcgttatg aagatgtacc agaaaagtta
    28381 tggaaaatct cagatatcac tgacttttgg ggaatcggca aacgaacagc aaaacgtctg
    28441 aaacaaatgg gtatacattc tgtttatgat ttagctcacg cggattatta tcacttgaaa
    28501 agcaagctag gggtcatcgg tactcagctt tatgcccatg cttggggcat cgatcgaagt
    28561 tttttgggac aagaatatac accaaaatca aaaagtatcg gaaatagtca aatcctagat
    28621 agagattata caagacgaga agaaatcgag atcgttatca aagaaatggc cgaccaagtc
    28681 ggcactcgct tgagacgatc tgcagcaaaa acacaagtag tcagcttatg ggtaggatac
    28741 tctttaggct atcttgatcc aaatggcaag accgggtttc accagcaaat gaaaatcgaa
    28801 gcgacaaatg ctagcagaga tctagcagat gctttattga ccattttcca ccagcattac
    28861 cactaccagg atattcggcg tgtaggcgtg aactgttccg acctcgtata cgcgacaggt
    28921 ttacagctta acctttttga agatcctgaa aagcagataa atgaagcgaa agttgatttt
    28981 gttgtcgata cgattagaaa aaaatacggc tttcaatcaa tcatccatgc gcatagtctc
    29041 ttgcaaggag gacgcgccat cgcacgtagt tctctcgttg gcgggcatgc aggtggtatg
    29101 agtggattag aaggtggaaa agatgagacg gacagcaaaa aaattcattg actataacga
    29161 gtatcgagat cggccatttg gattaaaatg ggggactgct tttgcgatgg aagaactagt
    29221 caacggtatc aaaaaaaatg aagaagaagc acgcaaacag cctcttccta aacaacaaat
    29281 gacattgcaa gaaatcgata gtatactgac agaagccttt ttatctcgta aagcagtaat
    29341 cattcagctc aatttaaaag atgaatttgg aagactcttg gatgaggtcg aagggcactt
    29401 cattggtgaa gcctatgaag attactttat gattgaccat cagccgatat tgtgggaaga
    29461 cgtacggtat attgagttaa aggaaacaaa aaaatggttt gtgacatgag aggttgtgac
    29521 agaagtgatc aacttcaaaa aataagaaag gatttttcga aaattgcttc ttaaattttt
    29581 gtggaatttt ggcttatttt tcgaagaagc aacttctgga acaacgtata ttagatatca
    29641 gaggttgtga aaaagtcgct tagttttgag taacaagcca aggcgctgat ttcacaacct
    29701 ctgtctatct atccatctat tcggtaagat tcatgcatga cgtcttcttt atttagtatg
    29761 tagagccctt tttcttctga ccattcaaca taaatcagct gttttacatc tgcatagcct
    29821 tctttcttca agttctccat aagccatgct tcagtcaatt gattggactg aagcacttta
    29881 tgctgaattt gcccttcatc tacaagcagg atcgataaag ctttgtcttc atctgatttt
    29941 ttcaatacac tcatttgacc ggcattctct aggataacat gtttggcatt ttcaagaaaa
    30001 aagcattctt gctgtcttaa gattgctcgc aactgctcca tctcgatatg gttatttgtc
    30061 aactctgtca tgttgatgac gccgtctttg attagaacag atggctcgcc tttgacccaa
    30121 cgattgacct tttcattttt ttggacgata cgttcgatac cataaatcat tactgcccaa
    30181 agcgcaatgg caaataggac atgccctaca tggacattgt catcataggt tgattcttct
    30241 aagatcccac ctaaaacaag cgtatagact aaatcaaacg gtgtgatttc tgacatggat
    30301 ttcttcccaa gcaaacgtac cactaataat aaaccgatca acccgaacac cagtttcatt
    30361 gtaattgaaa aaatcatttt ttctccccct aactatctgt taattaaatt atatatgctt
    30421 tctcacgaac acgcatggaa tatacaagga agaaatgtat ccttttacta aaaaaaggac
    30481 tgtgacactg tagtgacccc caaaagttag acttttttgg agtggagaaa tccactcctt
    30541 ttttttatgc tctaaaatga tatttttaca caaccgttac ggttgtttta cgaaattgga
    30601 cg
//
LOCUS       DACCCR010000029        30346 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCCR010000029 DACCCR010000000
VERSION     DACCCR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.030"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            172..516
                     /locus_tag="IVK93_001313"
     CDS             172..516
                     /locus_tag="IVK93_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP6386613.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="IVK93_001314"
                     /pseudo
     CDS             517..936
                     /locus_tag="IVK93_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="IVK93_001315"
     CDS             complement(1028..1378)
                     /locus_tag="IVK93_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386614.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="IVK93_001316"
     CDS             complement(1344..2675)
                     /locus_tag="IVK93_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP6386615.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="IVK93_001317"
     CDS             2931..3497
                     /locus_tag="IVK93_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6386616.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="IVK93_001318"
     tRNA            3642..3729
                     /locus_tag="IVK93_001318"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /locus_tag="IVK93_001319"
     CDS             4063..5037
                     /locus_tag="IVK93_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6386617.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="IVK93_001320"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="IVK93_001320"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP6386618.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="IVK93_001321"
     CDS             complement(7452..7898)
                     /locus_tag="IVK93_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6386619.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="IVK93_001322"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="IVK93_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6386620.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="IVK93_001323"
     CDS             8363..8881
                     /locus_tag="IVK93_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6386621.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="IVK93_001324"
     CDS             complement(8958..9914)
                     /locus_tag="IVK93_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6386622.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="IVK93_001325"
     CDS             10080..10886
                     /locus_tag="IVK93_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6386623.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="IVK93_001326"
     CDS             10883..11872
                     /locus_tag="IVK93_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6386624.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="IVK93_001327"
     CDS             11869..12873
                     /locus_tag="IVK93_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6386625.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="IVK93_001328"
     CDS             13005..13547
                     /locus_tag="IVK93_001328"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP6386626.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="IVK93_001329"
     CDS             13567..14265
                     /locus_tag="IVK93_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP6386627.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="IVK93_001330"
     CDS             14286..14498
                     /locus_tag="IVK93_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP6386628.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="IVK93_001331"
     CDS             14498..15460
                     /locus_tag="IVK93_001331"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP6386629.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="IVK93_001332"
     CDS             15478..15873
                     /locus_tag="IVK93_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6386630.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="IVK93_001333"
     CDS             16036..16401
                     /locus_tag="IVK93_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP6386631.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="IVK93_001334"
     CDS             16398..18209
                     /locus_tag="IVK93_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP6386632.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="IVK93_001335"
     CDS             complement(18271..18438)
                     /locus_tag="IVK93_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP6386633.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18686..19675
                     /gene="galE"
                     /locus_tag="IVK93_001336"
     CDS             18686..19675
                     /gene="galE"
                     /locus_tag="IVK93_001336"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP6386634.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19687..21171
                     /gene="galT"
                     /locus_tag="IVK93_001337"
     CDS             19687..21171
                     /gene="galT"
                     /locus_tag="IVK93_001337"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP6386635.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21195..22175
                     /locus_tag="IVK93_001338"
     CDS             21195..22175
                     /locus_tag="IVK93_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6386636.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22264..23088
                     /gene="tsaB"
                     /locus_tag="IVK93_001339"
     CDS             22264..23088
                     /gene="tsaB"
                     /locus_tag="IVK93_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP6386637.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23072..23614
                     /gene="rimI"
                     /locus_tag="IVK93_001340"
     CDS             23072..23614
                     /gene="rimI"
                     /locus_tag="IVK93_001340"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6386638.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23621..24085
                     /gene="rimI"
                     /locus_tag="IVK93_001341"
     CDS             23621..24085
                     /gene="rimI"
                     /locus_tag="IVK93_001341"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6386639.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24082..25098
                     /gene="tsaD"
                     /locus_tag="IVK93_001342"
     CDS             24082..25098
                     /gene="tsaD"
                     /locus_tag="IVK93_001342"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP6386640.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(25707..26408)
                     /locus_tag="IVK93_001343"
     CDS             complement(25707..26408)
                     /locus_tag="IVK93_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6386641.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            26869..28098
                     /gene="arcA"
                     /locus_tag="IVK93_001344"
     CDS             26869..28098
                     /gene="arcA"
                     /locus_tag="IVK93_001344"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP6386642.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            28189..29208
                     /gene="argF"
                     /locus_tag="IVK93_001345"
     CDS             28189..29208
                     /gene="argF"
                     /locus_tag="IVK93_001345"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP6386643.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            29323..30270
                     /gene="arcC"
                     /locus_tag="IVK93_001346"
     CDS             29323..30270
                     /gene="arcC"
                     /locus_tag="IVK93_001346"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP6386644.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
BASE COUNT         9920 a         5198 c         6318 g         8910 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggacgggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgcat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc attttttatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatccttgaa
    18541 aatgtttact aaatgtttta ttataaattt accatatgaa tttacgaaat tttactttgt
    18601 ttgttttcac cataaaagta aacttcgttt tttaaattat atagaatcaa caaacacaat
    18661 tttactaatc aaaggagtgt tttttatgac aattttagta ctaggcggag ccggttatat
    18721 cggttcgcac gctgtagacc agctagttca aaaggggtat caagtagctg tggtagataa
    18781 tttgctcaca ggtcacaaac aagcagtaca tccagatgct catttttatg aaggagacat
    18841 ccgggataaa gaatttctac gttccgtatt tgaaaaggaa ccgattgaag gagtaatcca
    18901 ttttgctgca agctctttag taggagaatc tgtcgaaaaa ccattgatgt atttcaacaa
    18961 caacgtatac ggtatgcaaa tcttacttga agtcatgcac gagttcaacg tgaataaaat
    19021 cgtattctct tctactgcag caacttatgg agagccgaaa gagtctccaa tcacagaaga
    19081 tacaccagct aatcctaaaa atccgtatgg tgaaagtaaa ttgatgatgg aaaaaatgat
    19141 gaagtggtgt gaccaagcat atggcatgcg ctatgtcgct ttgcgctact tcaatgtagc
    19201 cggagcaaaa gcagatgcat ctatcggaga agatcataca ccagaaacgc atcttgtccc
    19261 aatcatttta caagtagctc ttggtcaaag aaaagccttg gctgtttatg gagacgatta
    19321 tgatacacca gatggaacat gtatcagaga ttatgttcaa gtagaagatt tgattgctgc
    19381 acatatttta gctttggaat acttgaaaga aggaaatgaa agcaacttct tcaatttagg
    19441 cagcagcaaa ggatactcag tcaaggaaat gctagaagca gctagagaag tgacaggaaa
    19501 agaaattcca gcagaaatag caccaagaag agctggagat cctagccgat tagtcgcttc
    19561 aagtgaaaaa gcgagagaga ttctgggctg gaaacctgaa tacacagata tcaaagcgat
    19621 tatcaaaaca gcatgggatt ggcatgtcag ccatccaaat ggttatgaag aataggggaa
    19681 tgagatatgt caatcagtca aacgatttgt gattttgcca cacttgcgat cgaagctggc
    19741 ggatggatgg aaatggaccg cttatattta cagaatcgtc ttttagggat ggtaggagaa
    19801 gatgctctag aaagcattga agtaagacca gttaaggaag aatctgtaga cttattggat
    19861 cagcttgtcg ctaaggcaag agaaaatggt gttctttcag aattgccagc agaacaagag
    19921 atttttgaag cgcaattaat ggattttctc acaccgccac cttcagtcgt caatgcattc
    19981 tttgcacagc actattcgaa aagcccagag gaagcaactg agtatttctt taatttaagt
    20041 aagaaaaatg actacatcaa aacaagagcc atcgcaaaaa acatcaaatt tccggcagag
    20101 tcggagtatg gtgaactgga aatcacaatc aatttatcta agccagaaaa agatccaaaa
    20161 caaatcgcag cagaacgcga ggcggtaaaa gtagactatc ctacctgcat gctttgcatg
    20221 gaaaacgaag gatatagagg tcgcttgaac tatccagccc gcaccaatca ccggattatt
    20281 cgaatgaatc tagacggtga aagctgggga ttccaatatt ccccttatgc ttattacaat
    20341 gaacattcga tcattttatc agaacaacac cgcccgatga aaatcacaaa ggaaacattt
    20401 gctagactgc ttcggattac tgaagtcttg cctcattact ttgttggttc gaatgctgac
    20461 ttaccaattg ttggaggatc gattctttct catgatcatt atcaggcagg acgtcatgaa
    20521 tttcctatgg caaaagcacc gatagaaaaa cttatcaaac tagaagatta tccgcaagtc
    20581 atcgcaggag tcgtgaaatg gccgatgtct gtgatccgtt tgcaatcaac agagaaaaac
    20641 ttgcttattg aagcagctaa ctcagtattg gaaaaatgga aagcatattc ggatgaaaca
    20701 gtatctatca gagctttttc agcagatggt acaccacacc atacgattac gccaattgca
    20761 cgaagaaggg acaattactt tgagttggac cttgtccttc gagacaacaa tgtttcagaa
    20821 gaacatcctg atgggatttt tcatccacat aaagatgtcc aacatatcaa aaaggagaac
    20881 attgggttga tcgaagtaat ggggcttgct gttttaccac ctcgtttgaa accagaacta
    20941 gtagaagtag agcatttctt attaggagaa gaaaaccaga tggctgaata tcatcgtccg
    21001 tgggcagaac aattaaaaaa agaacatcct gatttgacga aagatacggt caccgatgtc
    21061 gtacaaaaat ctgtaggtca ggtttttgca cgtgtactgg aagacgctgg agtattcaag
    21121 cgagatgaaa aaggacaggc agcgtttgaa cgatttactg cgattttata aacgtaaaaa
    21181 aggaggagag acaaatggca acaattaaag acattgcgca gttggcagga gtttctcctg
    21241 ctactgtttc aagagttctc aactatgatc ctgatctttc cgttggctta gccacaaagc
    21301 aaaaaatttt tgaagcagca gaacgcttaa attatacgaa acatcgtaaa aatgcagcaa
    21361 atactaagca aacgcttcgc ctgatccagt ggtacgacga tacggaagag cttgaagatc
    21421 tttattattt atctattcgc ctaggaatcg aaaaaaaggc agaagaagca aacgttactt
    21481 tattgaaaga aacgtgggat tccattagtg atcttccggc agatggaacg atcgcactag
    21541 gaaaattcga cgaagctcag ttattagaaa tgaaaggatc ccaaaatgcc ttgcttttgg
    21601 tagattctga cggcacttct caaggaatcg actctctagt tgtcgacttt aagtcaagtg
    21661 ttcaaaaagt cattgaacac tttgtctccc ataaagcgca acatatcgct atgcttgctg
    21721 gaacagaata tacgaagaaa aaccatcagc ggctaaaaga tcccagaatt atggcatttc
    21781 aacaagtttt ggcagaaaaa ggctacgacg aaggtccaat tatcgaagca gatttttctg
    21841 tcgagtcagg atatcaagca gtgaagcagt atttaaaaac gaatccgcat gttcctgatg
    21901 cgctttttgc agctaatgat gcattagcga ttggcgcact gaaagcgatt caagaaaaag
    21961 gtttagtggt accagaagat atttctgtga tgggcttcaa tgatgtcagt gtagcgaaat
    22021 atgtaacgcc ctcgctttct actgtaaaag tttataccga atggatggga gagctagcag
    22081 tggagacgat tcttcaatta agtaattcca aagcaccggt ttcaagaaag attattgtgg
    22141 aaacagagtt gattattcga gaatcaacga aataaaagat ggaaaagctg tgggaaaatg
    22201 gagtgatacc gcagcttttt tgtgtttcag gaaggggcaa aatttgcccg ccagcagata
    22261 atcatgtatt attgtactag ttttgccttt ggtattttca tcctcagaat aaaaaagtat
    22321 gctacaatgg aaggcgagaa tgtcatagca gaaaggatca aatatatgat tacattagga
    22381 atagatactg caaaccaaac tttggcgatc ggtgtggtag aagacgaaca aattcttgga
    22441 caaatacaaa caaatatcaa acgcaaccac agtgtaacac tgatgcctgc aatcgaccaa
    22501 ttgtttgcag atcttcaaat aagtccaaaa gacattgacc gaatcgcggt ttctgatggt
    22561 cccggttctt atacagggct tcgtataggt gtaacgacag caaaaacgat tgcttataca
    22621 ttagataaag agctagtagg agtatctagt ctgaaaacca tcgcggctaa ttgcatcggt
    22681 gtggataata ttattgtgcc catgtttgat gctcgaagaa aaaatgtata cgctggggca
    22741 taccgctgga aagaaaatag attagaaact gtacttgaag atgtacatat ttctgcttct
    22801 gatttgttca cacaactaca aaagctgaat gaagctattt atttcgtcgg gagtgattgt
    22861 cacaaattct tctctgaaat aaaggaaata ttgcctagtg cgatgattaa tacagttcca
    22921 gcatgggata ttccaagtgg tgtaactgtg gctcaattag gtgctttagt tgaaccagtt
    22981 gcagagaaac aagcttttct tcctcgctat ttaaaacgag tagaagcaga agaaaaatgg
    23041 ttggagacgc acacacctgg agacgaaagt tatgttgaaa aaatttagag cgtatgccag
    23101 aagctttttt caagataaaa tgatttatac agaaaaaaca gtcgaacaaa tcgatcgtcg
    23161 ttacgtacta agagaattga ctacagaaga tatcaaagag ctgctggatg tcgaacgaga
    23221 agtctataat ggcgaattgc catggacaaa aagtgccttt atatcagaat tgaagtcacc
    23281 gcttcctcat ctatatctag gtatctttga taatggaaga atgattggtt ttatcggttc
    23341 aagagcgttc ggtttagatt gccatatcac gaatattgcc gttcgaccgg catatcagcg
    23401 taaacgaatc ggttcgctgc ttattgacga aatcgagaac tttgctataa tgaatcgctg
    23461 tgagacgatg tcattagaag tgcgtatgag taatcaagat gcacaaagac tttatcggaa
    23521 attggggttt caagcaacaa cgatcaaaaa aagatattac actgaaacaa aagaagatgc
    23581 gttggatatg ataaaacttt tagaaaatga gtgagattag ttgatttgca gaaaaaaaga
    23641 ttttgatgaa gcgtacttgg cagaatgcgt atggatgatt agcgaaaatg cttatccaca
    23701 tggttcacct tggacgaaaa tgcagttttt ggcagacatc caacagcctc actctgatta
    23761 tctgctttta gctgaagata accagatctg tggctttatt ggatactcga aagtaataga
    23821 tgaagtcgag atcacaaata ttgctgttgc agtttcagaa caaggaaaag ggcatgcacg
    23881 gcatttgtta caattactga ttacggaaca aataaaagat tcgctgcatg tttttttaga
    23941 agtacgtcta tctaatgaag cagcaagaaa actttatgaa tcagaaaagt ttcgtatatt
    24001 aggtaaacgg aaatcatatt atcgcaatcc gacggaagat gcgatcatta tgagcagaaa
    24061 gttaaaaacg gagacgatga aatgaatgaa gaattgattc tggcgattga atcaagttgt
    24121 gatgaaacga gtgtagcggt cgttagaaat ggaacagaga tcttatctaa catcgttgct
    24181 tctcaaataa acagtcataa acgttttggt ggcgtagtac ctgaagtagc tagccgccat
    24241 cacgtagaac aaatcacctt atgcttggaa gatgcactgg tagaagctgg tgtgtcggca
    24301 gaagatttat cggcagtcgc tgtcacttat ggaccaggac ttgtcggctc gttgctgatt
    24361 ggtatttctg cagctaaggc ttttgcttgg gcacaccaat tgccgcttat tcctgtaaac
    24421 catatggcag ggcatatcta tgcagcccgt ctggttaaac cattccagtt tccattgatg
    24481 gcacttcttg ttagcggagg acatacagaa ttagtctata tgcaagaaga tggatcatat
    24541 gaaatcattg gggagacaag agatgacgca gcaggagaag cttatgacaa agttggaaga
    24601 gtattaggtc tttcttaccc aagcggaaaa gaaatcgatc agctagctca tcagggaaaa
    24661 gataattacc attttccgcg agcgatgatc catgaagata attatgattt cagttttagc
    24721 ggattaaaaa gtgcatttat caatttggtg cataatgcac aacagcgtgg agaagatcta
    24781 gacaaaaatg atttagcagc tagctttcaa gcaagtgtga tcgatgtatt gattactaaa
    24841 acacttcgag cttgtcaaaa ttatccagtc aagcaactga ttatagctgg aggtgtagcg
    24901 gccaatcaag ggttgcgtga aggattgcag gatgctttgg caactaaact ccctgaggta
    24961 gaactagtga ttccccctct tcgactttgt ggagataatg ctgcaatgat tggagcagca
    25021 gcacatgtgg aaatgcaaaa aagaaatttt gctagctatc agttgaatgc tgatccaagc
    25081 ttggttttat ctgaataaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    25141 gcccaggaaa aacggcttct catgtttttg acctggctgt tcttattacc gcgtatgcaa
    25201 gcttttgaac gccgtttaat caagagttcg taagcctgtc gtttaactct gattcacaag
    25261 gaacaatttc aaaaaacagc tcctcatgtt tatgaacatt atgacttgtg accgaagagt
    25321 tgacaggtac acaccgttta atcgaaatag ggctgtgaca cgtttttgtc aaagccctat
    25381 tttttacata cttttaatca ttattgttat tttgctattt tttacagctt atttatcatt
    25441 atccgttcct ctgattgccc tcactgagta atcgtttatt gtcgaggaat atcgaataat
    25501 ctagatcgga tgaggacaag acgcttcatc tagttaatct aacagtattt tttgcaaaca
    25561 gtgaatatta cataactgaa acacacatag aaaatctttg agtcttgtcc agtgatctaa
    25621 aactgccgga tctttcagaa gttttaggga ggaaaataag cagtgaccct gatatttatc
    25681 agggtcactg cttatttttg ttcgcgttat tctctcatag ctggagtagt agctgtctta
    25741 cagaaagtaa aacgcttttt gtcataatct aacagcccgg aatcacgtag ttgtttgatt
    25801 acttgtccag cagtttcacg tgtcgtgccg ctattcactg ctaattcttt taaggtgatc
    25861 ggataaggaa tggtgatatg accatctttt gttggtacgc ccaattcttc tttcaaaatg
    25921 aataaatttt gttttacccg gtcaaaggcg ctagaggtca atccgatttg gatacgcaat
    25981 tcatgtattt caactacttt agaaattttt tgtatcatat agatcaactg ttcgggattt
    26041 tttcgtgcta atttttcata gtaggaaaca ggaaggtaga atgtttcaat atcagtcatc
    26101 gcttgagctg tataagtgta ggtctcttct ccaaacattc ctgacatagg aaaaaggtta
    26161 tttgtattca catagtcata gtagtaataa gttgcacttt catcataacg ttctaaacga
    26221 acgagtcctt tggtcaagat atacaaccgt tgtctgggat caccatcgtc gaaaaggatc
    26281 tgtccttttt tataagaacg aagaacagta tgttccttga tctgttggaa ctcttcatcc
    26341 gagaaatgtt caaaatcagg ttgatatctg agttttgaaa agtgaaaatc gattttatta
    26401 acacgcatta aaaagtcctc tccttattgt tcactatatt tattgtcact ttttttaaag
    26461 aatttgtaaa gttttaacat accaacatta gaaacaagtt ttaatacttt acaaatataa
    26521 tttgttatta tgtaaaaaat atacaatggt attgaataat ttgttttgaa gcgaatgatt
    26581 tgtgtataaa aaacaacaat aatacatata gtaaacgcta acaacgataa tataatttat
    26641 ttttttaatt tattcaaaaa aattttttca atgaataaat atttttttga aagtgtatac
    26701 agaataaaaa atgaataaat tacgcttaaa aatagaaaaa cgtttaatta tttaattatc
    26761 gaatattaat gtgaggaatg tctcattttg aatgagacaa aattatgaaa gtgattacaa
    26821 aaggaggata tcctatacat gtaatcaaaa agaaggaggc aacacaacat ggataaacct
    26881 attcacgttt tctctgaaat agggaaatta aagacagttt tactgaaacg gcctggacaa
    26941 gaagtggaaa atttgacacc agatattatg gatcgtctgt tgttcgatga tattccatat
    27001 cttccgattg ctcaagaaga acatgacaac tttgcgaaga cattgcaaaa tgaaggcgtc
    27061 gagactcttt acttggaaaa attagcggca gaagcgattg acgcaggcaa tgtcaaagaa
    27121 caattcttga ataaaatgtt ggatgaatct catattgcat cgaatgctgt aagagagggt
    27181 cttcatgagt tccttctatc aatggaaacg caagaaatgg tagataaaat catggctggg
    27241 gttcgtacaa aagatatcga agtacgttct agcagtctat atgatctgtc agcagacgat
    27301 gactatccat tttacatgga cccaatgcca aacttgtact ttacacgcga cccttccgca
    27361 tcaatgggaa atgggatgac agtcaataag atgacttttg aagcacgtcg tcgtgaatcg
    27421 atgtttacag aatatatttt gaaacatcat ccaagatttg caaataaagg aatcgaagta
    27481 tggttggatc gtgaaaatcc agatcatatc gaaggcggag acgaattgat cttaagcgat
    27541 aaagtagtag ctgttgggat ttctcaacga actaatgcga aagctttaga aaaattagca
    27601 cgccacctat tcgcaaaaaa ttcaggattt gaaaaagtgt tggcaatcaa aattccgaac
    27661 aaccgtgcaa tgatgcattt ggataccgta tttacaatgg tagatcacga taaattcact
    27721 atccatccag caattcaaag taaagacggt aagatggatg tctttacaat cgaaccagat
    27781 ggtgatgata tcaagattac tcattcagat aacttacacg aaacgatgaa agctgctctt
    27841 ggattagatg atcttgtact gatcccaact ggtaatggag atgagatcgt tgcacctcga
    27901 gaacaatgga atgatggatc aaacacattg gcaatcgctc caggtgttgt agtcacttat
    27961 aaccgaaatt atgtatcgaa cgaattgctt cgtagctatg gcatcaaagt gttagaaatc
    28021 aattcaagtg aattgtcacg aggtcgtggt ggtccacgct gcatgagcca accacttgtc
    28081 cgtgaagact tgaagtaatt tgtttcctga cggcagtctt actgtcagct gccgtcggga
    28141 tttcctcttt aatcttaaat aaaaacaatc taaaggagaa tgtttaatat gaaagaatca
    28201 gtattccaag gaagaagctt attagcagaa aaagacttta caaaagaaga attacaatat
    28261 ttgatcgatt tttcagaaca cttgaaagac ttgaaaaaac gtggtatccc acatcactat
    28321 ctagaaggaa aaaatattgc gcttttattt gaaaaaacat ctacacgtac tcgttctgca
    28381 tttactactg cagcgatcga tctaggtgca catcctgaat atctaggtgc aaatgatatc
    28441 caattaggta aaaaagaatc cacagaagat acagctaaag tactaggacg tatgtttgac
    28501 ggaatcgaat tccgtggatt cagtcaaaaa atggtagagg aactagcaga attctctggt
    28561 gttccagttt ggaatggctt gactgatgaa tggcatccaa cacaaatgat cgctgacttt
    28621 ttaactatcc aagaaaactt tggaacagta gaaggtatca ctgttgctta ctgcggagat
    28681 ggtcgaaaca atatggctaa ctctttatta gtcacaggtg caattttagg agcgaatatg
    28741 cgtatcgttg caccaaaaga attacaacct gaagaagaaa tcgtcaaaat ggcagaaggc
    28801 tttgctgaaa aatccggtgc acaattgatg attaccgacg atgttgataa aggtgtagac
    28861 ggagcagacg tcctttattc tgatgtatgg gtatcaatgg gtgaagaaga taaattcgaa
    28921 gaacgtatca aattgttgaa accttaccaa atcaatatgg aaatggtaga aaaaacacac
    28981 aacacagatc gtttgatctt cttgcactgc ttgccagctt tccatgatac aaacactgtt
    29041 tacggtgaac aaatgaaaga acgctttggc atcactgaaa tggaagtaac agatgaagtc
    29101 ttccgcagca aatatgctcg tcaatttgac caagcagaaa atcgtatgca ctcaatcaaa
    29161 gcgatcatgg cagcaacatt aggtaacttg tttatccctc gcgtttaagc ataaccacat
    29221 tcatctcacg aaaaaacgag tcagcattga gggaaaatca caaaacgctg gctcgttttg
    29281 ttatctagaa aattcggtag aactgaagaa aggacgaatg aaatggccaa acgaaaagta
    29341 gttgtcgctt tagggggcaa tgccatcttg tctacggatg caagtgcaaa agcccaacaa
    29401 gaagcgttaa tggaaacagc aaagtattta gtgaaattta tcgaacaagg agatgaattg
    29461 atcatctccc acggaaatgg tccgcaagtc ggtaacctat tgattcagca gcaagctgct
    29521 gattctgaga aaacaccggc aatgccttta gatacatgtg ttgcaatgac agaaggatct
    29581 atcggttact ggttacaaaa cgccatgggc gaagtactga aggaaaaagg gatcgataaa
    29641 gatgtggttt ctttagtcac tcaagttatt gtggatgaaa atgatccttc attcaaaaat
    29701 ccaagtaaac cagtcggtcc attctacacg gaagaagaag ccaaagaaca aatgaatgct
    29761 gactccactg tgacattcaa agaagatgct ggacgtggat ggagaaaagt agtcgcttcc
    29821 cctaaaccca tctcgattaa agaagcacgt gtcatcgaaa cgctggtcga tcaaggagtt
    29881 atcacagtct ctgttggtgg cggtgggatt cctgttgtag aaacagctac tggattagaa
    29941 ggacgagaag ccgtaatcga taaagatttt gcttcagaaa aattagccga aatcatcgat
    30001 gcggatttgc tgatcgtatt gacaggagta gacaatgtgt atgtcaatta ccaaaaacca
    30061 gatcaaaaga aattagaaac tgttactgtt tcagaaatga aacaatacat tgatgaaaag
    30121 caattcgctc caggaagcat gcttccgaaa gtagaagcag ctattgcatt tgttgaagca
    30181 aaaccaaacg ctaaggcaat catcacttct ttagaaaata tcgagaatct tttagcttct
    30241 gaagaaggaa cgattatcgt agctgattaa tttgttaagt ctttcgtatt tacgaaatga
    30301 tttatagcta tccaggtggt atttcttctc ctttaggcca tctgga
//
LOCUS       DACCCR010000030        28599 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCCR010000030 DACCCR010000000
VERSION     DACCCR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.031"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            237..1877
                     /locus_tag="IVK93_001347"
     CDS             237..1877
                     /locus_tag="IVK93_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP6386645.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="IVK93_001348"
     CDS             2031..3353
                     /locus_tag="IVK93_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP6386646.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="IVK93_001349"
     CDS             complement(3415..3846)
                     /locus_tag="IVK93_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6386647.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="IVK93_001350"
     CDS             complement(3910..4746)
                     /locus_tag="IVK93_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP6386648.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="IVK93_001351"
     CDS             complement(4936..5238)
                     /locus_tag="IVK93_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP6386649.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IVK93_001352"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IVK93_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP6386650.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="IVK93_001353"
     CDS             complement(5692..5958)
                     /locus_tag="IVK93_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP6386651.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="IVK93_001354"
     CDS             complement(6212..6937)
                     /locus_tag="IVK93_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP6386652.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="IVK93_001355"
     tRNA            complement(7303..7373)
                     /locus_tag="IVK93_001355"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="IVK93_001356"
     CDS             7490..8242
                     /locus_tag="IVK93_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP6386653.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="IVK93_001357"
     CDS             8258..8737
                     /locus_tag="IVK93_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP6386654.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="IVK93_001358"
     CDS             complement(8988..10568)
                     /locus_tag="IVK93_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP6386655.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="IVK93_001359"
     CDS             complement(10561..11304)
                     /locus_tag="IVK93_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6386656.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="IVK93_001360"
     CDS             complement(11480..12256)
                     /locus_tag="IVK93_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6386657.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="IVK93_001361"
     CDS             complement(12504..13340)
                     /locus_tag="IVK93_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP6386658.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="IVK93_001362"
     CDS             complement(13351..14280)
                     /locus_tag="IVK93_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP6386659.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="IVK93_001363"
     CDS             14472..14825
                     /locus_tag="IVK93_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6386660.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="IVK93_001364"
     CDS             complement(14899..15555)
                     /locus_tag="IVK93_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6386661.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="IVK93_001365"
     CDS             15781..17715
                     /locus_tag="IVK93_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6386662.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="IVK93_001366"
     CDS             complement(17801..19024)
                     /locus_tag="IVK93_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP6386663.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="IVK93_001367"
     CDS             complement(19018..20916)
                     /locus_tag="IVK93_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP6386664.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="IVK93_001368"
     CDS             complement(20910..22028)
                     /locus_tag="IVK93_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6386665.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="IVK93_001369"
     CDS             complement(22111..22944)
                     /locus_tag="IVK93_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6386666.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="IVK93_001370"
     CDS             complement(22937..23854)
                     /locus_tag="IVK93_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6386667.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="IVK93_001371"
     CDS             complement(24081..25205)
                     /locus_tag="IVK93_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6386668.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="IVK93_001372"
     CDS             25388..26296
                     /locus_tag="IVK93_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6386669.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="IVK93_001373"
     CDS             26437..28050
                     /locus_tag="IVK93_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP6386670.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8889 a         5621 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattt
//
LOCUS       DACCCR010000031        26494 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCCR010000031 DACCCR010000000
VERSION     DACCCR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.032"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(99..1229)
                     /locus_tag="IVK93_001374"
     CDS             complement(99..1229)
                     /locus_tag="IVK93_001374"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP6386671.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVK93_001375"
     CDS             complement(1878..3080)
                     /locus_tag="IVK93_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6386672.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVK93_001376"
     CDS             complement(3140..3343)
                     /locus_tag="IVK93_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6386673.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVK93_001377"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVK93_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVK93_001378"
     CDS             4880..5692
                     /locus_tag="IVK93_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6386674.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IVK93_001379"
     CDS             complement(6716..6868)
                     /locus_tag="IVK93_001379"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386675.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IVK93_001380"
     CDS             complement(7059..7937)
                     /locus_tag="IVK93_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6386676.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IVK93_001381"
     CDS             complement(7976..9094)
                     /locus_tag="IVK93_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP6386677.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IVK93_001382"
     CDS             complement(9091..11190)
                     /locus_tag="IVK93_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP6386678.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IVK93_001383"
     CDS             complement(11241..12062)
                     /locus_tag="IVK93_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6386679.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IVK93_001384"
     CDS             complement(12059..12868)
                     /locus_tag="IVK93_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6386680.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IVK93_001385"
     CDS             complement(12889..13383)
                     /locus_tag="IVK93_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6386681.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IVK93_001386"
     CDS             complement(13395..13883)
                     /locus_tag="IVK93_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6386682.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IVK93_001387"
     CDS             complement(13960..16656)
                     /locus_tag="IVK93_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6386683.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IVK93_001388"
     CDS             complement(17084..18157)
                     /locus_tag="IVK93_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6386684.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IVK93_001389"
     CDS             complement(18154..18981)
                     /locus_tag="IVK93_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6386685.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IVK93_001390"
     CDS             complement(18982..19788)
                     /locus_tag="IVK93_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6386686.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IVK93_001391"
     CDS             complement(19800..20885)
                     /locus_tag="IVK93_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6386687.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IVK93_001392"
     CDS             complement(20902..21444)
                     /locus_tag="IVK93_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6386688.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IVK93_001393"
     CDS             21702..22691
                     /locus_tag="IVK93_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP6386689.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IVK93_001394"
     CDS             22688..23425
                     /locus_tag="IVK93_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP6386690.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IVK93_001395"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IVK93_001395"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP6386691.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNTITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IVK93_001396"
     CDS             23989..24621
                     /locus_tag="IVK93_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6386692.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IVK93_001397"
     CDS             24706..25353
                     /locus_tag="IVK93_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6386693.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IVK93_001398"
     CDS             25358..26341
                     /locus_tag="IVK93_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP6386694.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8107 a         5592 c         4493 g         8302 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaatg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaacac gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcg
//
LOCUS       DACCCR010000032        25350 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCCR010000032 DACCCR010000000
VERSION     DACCCR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.033"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(124..501)
                     /locus_tag="IVK93_001399"
     CDS             complement(124..501)
                     /locus_tag="IVK93_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386695.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            757..1485
                     /locus_tag="IVK93_001400"
     CDS             757..1485
                     /locus_tag="IVK93_001400"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP6386696.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKAQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1485..1739
                     /gene="purS"
                     /locus_tag="IVK93_001401"
     CDS             1485..1739
                     /gene="purS"
                     /locus_tag="IVK93_001401"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP6386697.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1741..2412
                     /gene="purQ"
                     /locus_tag="IVK93_001402"
     CDS             1741..2412
                     /gene="purQ"
                     /locus_tag="IVK93_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP6386698.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2413..4635
                     /gene="purL"
                     /locus_tag="IVK93_001403"
     CDS             2413..4635
                     /gene="purL"
                     /locus_tag="IVK93_001403"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP6386699.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4620..6059
                     /locus_tag="IVK93_001404"
     CDS             4620..6059
                     /locus_tag="IVK93_001404"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP6386700.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6091..7134
                     /locus_tag="IVK93_001405"
     CDS             6091..7134
                     /locus_tag="IVK93_001405"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP6386701.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7131..7709
                     /gene="purN"
                     /locus_tag="IVK93_001406"
     CDS             7131..7709
                     /gene="purN"
                     /locus_tag="IVK93_001406"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP6386702.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7706..9241
                     /gene="purH"
                     /locus_tag="IVK93_001407"
     CDS             7706..9241
                     /gene="purH"
                     /locus_tag="IVK93_001407"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP6386703.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9257..10531
                     /gene="purD"
                     /locus_tag="IVK93_001408"
     CDS             9257..10531
                     /gene="purD"
                     /locus_tag="IVK93_001408"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP6386704.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10653..11567
                     /locus_tag="IVK93_001409"
     CDS             10653..11567
                     /locus_tag="IVK93_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP6386705.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11700..13265)
                     /locus_tag="IVK93_001410"
     CDS             complement(11700..13265)
                     /locus_tag="IVK93_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6386706.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13258..14709)
                     /locus_tag="IVK93_001411"
     CDS             complement(13258..14709)
                     /locus_tag="IVK93_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6386707.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14723..15739)
                     /locus_tag="IVK93_001412"
     CDS             complement(14723..15739)
                     /locus_tag="IVK93_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6386708.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15959..17047
                     /locus_tag="IVK93_001413"
     CDS             15959..17047
                     /locus_tag="IVK93_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6386709.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17101..18627
                     /locus_tag="IVK93_001414"
     CDS             17101..18627
                     /locus_tag="IVK93_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP6386710.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18629..19834
                     /locus_tag="IVK93_001415"
     CDS             18629..19834
                     /locus_tag="IVK93_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6386711.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20091..21068
                     /gene="guaC"
                     /locus_tag="IVK93_001416"
     CDS             20091..21068
                     /gene="guaC"
                     /locus_tag="IVK93_001416"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP6386712.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21332..22756
                     /locus_tag="IVK93_001417"
     CDS             21332..22756
                     /locus_tag="IVK93_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6386713.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23217..24326
                     /gene="pdhA"
                     /locus_tag="IVK93_001418"
     CDS             23217..24326
                     /gene="pdhA"
                     /locus_tag="IVK93_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP6386714.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24329..25306
                     /locus_tag="IVK93_001419"
     CDS             24329..25306
                     /locus_tag="IVK93_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP6386715.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8196 a         4316 c         5695 g         7143 t
ORIGIN      
        1 aagcagatcc gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag
       61 gaacctgatt ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag
      121 gaattaagcg taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag
      181 caagttttcg agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata
      241 atgatttttg aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc
      301 tgtctgataa agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat
      361 ttttcggaat gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag
      421 caaatcatta tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc
      481 ttgttttatt gcatcttcca tcggcaagtg cctcctcgaa cccattatcc aaatttttag
      541 ttaattctaa tataccatca ttaagtataa atgtaaacgc ttgttgatgg tataagattt
      601 tcgtttctga taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg
      661 ttgatttata aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt
      721 atttgctttt aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat
      781 gaaggaaaag ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat
      841 ttagatcaag caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa
      901 ttgaataatc aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat
      961 cattttattg aacaattgtc taaaaccgaa caattagcaa aaaaagcaca aattatccct
     1021 ctggaagtgg ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc
     1081 gaaggtaaag aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat
     1141 gatccattta tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc
     1201 agtgagctga aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag
     1261 gtcggtttgg ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg
     1321 cttgcggatg aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat
     1381 atggacaaag acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta
     1441 tataaccgtc tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag
     1501 tatatgtaac ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag
     1561 tccatcgtat gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac
     1621 aacttgctga tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc
     1681 ttgcaaatgt aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac
     1741 atgaaatttg cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca
     1801 gttcgcgaga tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa
     1861 ggatttgatg gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt
     1921 gcgattgctc ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa
     1981 ccagtattcg gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt
     2041 gtactgagac gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac
     2101 cccaagacgg cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct
     2161 catggggaag gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat
     2221 cagattgcct ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt
     2281 acgaacaaag cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac
     2341 ttgctaggat cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag
     2401 gtgaccgtat agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga
     2461 atctatgccg aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta
     2521 ggaagaatgc ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt
     2581 tcctataaaa attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg
     2641 caaggccctg gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc
     2701 aaagcagaaa gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga
     2761 gttggcggaa tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat
     2821 tcattgaggt tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt
     2881 gcaggtatcg gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca
     2941 tttgatcctt gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa
     3001 cacaaacata tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt
     3061 gcaaaaacag gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa
     3121 gaagaagaac agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg
     3181 cttgaagcgt gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat
     3241 atgggagcag caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga
     3301 cttatattga cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg
     3361 atgttatcgg aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa
     3421 atccaagaac tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac
     3481 gacgggcaat atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca
     3541 ttagctgaag atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa
     3601 tttcagcagt tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat
     3661 ctgctgcagc aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa
     3721 gtacaaacca atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg
     3781 acgaataaag cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca
     3841 aaaatcggag gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt
     3901 aagcccttgg cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt
     3961 tgggaattat cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct
     4021 gttatttcag gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca
     4081 ccaatgattg gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc
     4141 aaaaaatcag gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt
     4201 gaattacaaa agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc
     4261 attgaaaaga gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt
     4321 gctcatgact gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat
     4381 cagctaggga tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa
     4441 tcaagatttg tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga
     4501 gatgctgctg ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact
     4561 gacggtcaga tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta
     4621 tgtcttatga agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc
     4681 cagacgccgc tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag
     4741 gtgcaggtat cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc
     4801 tttctgaggt attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg
     4861 gacatgtacg ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat
     4921 tcaaatttca tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt
     4981 cccttagaaa agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga
     5041 tattgatgca tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg
     5101 ctttgaatga agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg
     5161 ccgctttgga cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat
     5221 acgtgattgc ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg
     5281 tccagcctgg tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga
     5341 aggacacgca gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa
     5401 acatcgcagg gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag
     5461 aatcgccagt aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa
     5521 gcggttatgc ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca
     5581 ttgcccgaac atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat
     5641 tatctgctgt acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg
     5701 ttcggggtac gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag
     5761 tccatgtgag gattgcttct cctcctttga aatatccatg tttttacggg attgatattc
     5821 aaacgcgtaa agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg
     5881 gtgcagattc tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa
     5941 aagatgctcc gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt
     6001 tatatgacta tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa
     6061 agcagaccaa gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta
     6121 gatgtcgaag ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa
     6181 cgtttaggta tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt
     6241 caattaaaag agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg
     6301 gcaatcgaag cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat
     6361 gatatcttag cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa
     6421 aatgatccgt tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga
     6481 aatatggcgc tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat
     6541 tatgatctag ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa
     6601 acgatcaacc caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg
     6661 tactctcttg ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg
     6721 ccagaattga aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct
     6781 tccttgcttc cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga
     6841 gggtttgtag aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt
     6901 ggctcatggc cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag
     6961 atggagatgt atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg
     7021 aaagtaaaag aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg
     7081 aaagtgatcg ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg
     7141 ctgtttttgc ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa
     7201 aaggattgga atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga
     7261 aacgtgcaac tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa
     7321 aaaaagagta tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg
     7381 tattagctgg atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa
     7441 tcatcaatat ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg
     7501 cctttgaagc tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag
     7561 atactggacc aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt
     7621 ctttagaaga aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa
     7681 ttattgaaaa tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac
     7741 tgggattgta gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg
     7801 aggaacaaag catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac
     7861 agggtttcct gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg
     7921 tcttttagga agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca
     7981 accaatcgac tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga
     8041 tgtcgaaata gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag
     8101 tgcagcaaaa aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt
     8161 actttcagaa ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc
     8221 aaaagtcttt cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg
     8281 ggtggaagaa aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg
     8341 ttacggtgaa aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata
     8401 ttcgatcgca gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga
     8461 tgcagatgct gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa
     8521 acacatgaat ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc
     8581 gtatgaagca gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga
     8641 tctgccaaca gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta
     8701 tacacaagaa gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga
     8761 ctttgcgcag gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct
     8821 tgtacaagat caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg
     8881 taagccgact ccagcagaag caaaagcatt agcgtttgct tggagagctg tcaaacatgt
     8941 gaaatctaat gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca
     9001 gatgaatcga gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg
     9061 tgcagtttta gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa
     9121 acatggtata aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga
     9181 tatggcgaat aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta
     9241 gagaggagtc ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat
     9301 aagtaaaaaa cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc
     9361 ggggatgaca aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt
     9421 aatccatttt gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt
     9481 gttaaatggg atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa
     9541 agcagcagct ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca
     9601 aatcccgaca gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa
     9661 agaaaatggt gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt
     9721 cgtggctacc acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg
     9781 atttggggat agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc
     9841 ccttttggcg tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa
     9901 gcgggcattt gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt
     9961 accgcaaatt tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc
    10021 tttgggaatg aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc
    10081 tacgaaggaa ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca
    10141 agtcgttctt tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa
    10201 tcgtacagta gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc
    10261 tgccggttac cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga
    10321 gctttctgtt tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg
    10381 acgtctttat ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta
    10441 caccgcgtta atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa
    10501 agctgccaat agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca
    10561 acaaacataa tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact
    10621 tttgttgtgt atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg
    10681 atcagaaagc agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag
    10741 taaaagtgat tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt
    10801 atggaaacgg tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac
    10861 gagaagatct ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt
    10921 ctaaaaaaca tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg
    10981 tggatgcttt acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag
    11041 cgtttgatga attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac
    11101 caatgcaaat cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc
    11161 aatttggctt gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg
    11221 aagcaagtat cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga
    11281 tccaagcatg gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa
    11341 aattccctga attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa
    11401 ctggcttggc ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta
    11461 cgatgaacgt agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg
    11521 aagattggta cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag
    11581 caggtcaaaa gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg
    11641 agatattctg tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat
    11701 tagttatttt tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt
    11761 tttttcttaa ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata
    11821 tctgaaatac gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct
    11881 gtcagataat cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag
    11941 ggacttaatg caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt
    12001 tctttgataa aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt
    12061 gagggcagag cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg
    12121 tcggcgctga ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt
    12181 ttcttttctt tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat
    12241 cgtgttcgag ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg
    12301 tttttcgata atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga
    12361 agtttcgctt cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg
    12421 gtttcaagta tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg
    12481 acaggaccaa tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac
    12541 gtttgggcta cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa
    12601 aatttgatga atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa
    12661 aacttcaaat aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct
    12721 gctaataata cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc
    12781 aatgtttcac tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc
    12841 aattcgaaaa caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt
    12901 actgcttttt ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca
    12961 tctgcctgta cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat
    13021 atgagacgag tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg
    13081 aaaggcattt ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt
    13141 gccatctcac catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca
    13201 gttatttgtt cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta
    13261 tacatgtttg tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt
    13321 tccttctcgg tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag
    13381 gagacgcttg tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt
    13441 ggctaactct ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat
    13501 aactttgaat tggatcatct catcaatttt gcatgtggta atacgaataa atcctttgtg
    13561 acggacgtac ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt
    13621 taaaatagaa acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc
    13681 gtatcttatt tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg
    13741 aatccagtct tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat
    13801 agtcagagct tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt
    13861 ataaataaaa tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg
    13921 gtattgtttc aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat
    13981 gctagcggta agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt
    14041 ccctgaagaa agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct
    14101 ctctaaaaag cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc
    14161 cactattgtt aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc
    14221 gatctccaca cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac
    14281 ctgattgatg atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag
    14341 ctcgttttga taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat
    14401 ttctttaagt gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag
    14461 gatattttcc tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact
    14521 ttccaaaact ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat
    14581 cgtctgttga taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat
    14641 aacagcaaat agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt
    14701 ttttgtcatg atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct
    14761 gggaaaagac agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat
    14821 ttccaaaata acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga
    14881 ctacacaatt gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag
    14941 ttacaatgat caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac
    15001 cgtgagtcat ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt
    15061 ctaaatatcg tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt
    15121 gaacaatagt catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac
    15181 gattgattga ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac
    15241 cagcaagttc taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa
    15301 ataaacccgc ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct
    15361 cgggatcatt cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg
    15421 cttcttttaa tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca
    15481 ctttataggc aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat
    15541 ttcggtagag aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct
    15601 ttttccgcca gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg
    15661 cttcctttgt attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa
    15721 aaaataaaac acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca
    15781 gataaaaggg aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa
    15841 aaatccaaga caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa
    15901 tgttaacgct ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat
    15961 gaaaaacaaa tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt
    16021 aagtgcgtgt ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat
    16081 gccgaccaaa tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga
    16141 aaaactgggt tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc
    16201 acaaatagaa aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg
    16261 ttcggcttta actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta
    16321 tgatcgttta ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg
    16381 agtaggtgta tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg
    16441 accattcacg attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta
    16501 caacggagtg atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc
    16561 tggacagacg agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc
    16621 aaggatggat aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc
    16681 gccatatgat ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag
    16741 tgaaagtaaa cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc
    16801 gatcattgcc ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa
    16861 tacgattgaa atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga
    16921 aacttatgat aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga
    16981 taaagataat tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa
    17041 aaattaagag gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt
    17101 atggcggatt gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg
    17161 ttagattgcg tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac
    17221 ggagcgggaa aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat
    17281 gacggggaaa tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa
    17341 gaaaaaggca tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa
    17401 gagaatattt ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca
    17461 gaaaagaaaa catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta
    17521 gtatcccaga ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa
    17581 tccgtccgtc tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg
    17641 aatttgctga gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca
    17701 cataagctga atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag
    17761 acgattgaga cactagaaaa tgaaatgatc agcgaagagc atattattcg cggaatggtg
    17821 ggcagagatt tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt
    17881 aaagtagaag actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac
    17941 atcaacttgc atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc
    18001 agaactgaat tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt
    18061 atattaaagg aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga
    18121 ctcgcttatg tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg
    18181 gaaaacgtga cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa
    18241 aaagaagtaa ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc
    18301 catcaaattg tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg
    18361 ctgatgacac aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg
    18421 gcaaaatatg agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt
    18481 cttatctctt ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac
    18541 gaaggacgta ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa
    18601 ttaatgacga aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa
    18661 agagaaaaaa ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt
    18721 gatcatttta gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg
    18781 tccattgaat ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg
    18841 gatgttatta gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt
    18901 tgttggtgca attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt
    18961 tatcgcatgt cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta
    19021 tgcagggata ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac
    19081 acaagttgtt ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc
    19141 tacgggttat ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt
    19201 aggaatgatc atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa
    19261 aaatcattta tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc
    19321 agtcgttgta ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt
    19381 gatcattctt ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg
    19441 tcgacagatt tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac
    19501 aaagcgaatc acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt
    19561 agttttagct gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga
    19621 tgcaatggcg gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat
    19681 gggtgcgatt gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg
    19741 cgtcggggta gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct
    19801 agacttgtat aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag
    19861 actgtgacaa gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg
    19921 tttgtataga ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct
    19981 cttgattttt ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa
    20041 attaataatg ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat
    20101 ttgattacga agatattcaa ttaattccta ataaatgtat cgtaaatagc cgttctgaat
    20161 gtgatacaac ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca
    20221 tgcagacgat tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata
    20281 tcatgcatcg gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg
    20341 gattgattac ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt
    20401 tggcagaaga atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta
    20461 actcagttat tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg
    20521 ccggtaatgt tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa
    20581 caaaagtcgg gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga
    20641 ctggaggctg gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca
    20701 tcgctgacgg tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga
    20761 caatggtcat gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag
    20821 tcgaaaatgg aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg
    20881 aaaaacgaaa tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata
    20941 cactagtgga aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc
    21001 tggaagccat tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca
    21061 caatctaaaa aataaataaa atattaggat gttgtgaaaa agttgtcctg cattaagtaa
    21121 taagaacagc caaacaaaaa tagttcttta tatgttttgt ttggctggaa ttattatcgc
    21181 agatacaagc ttttgaacat cattattcag aataagggac tgtgataaga tttttgtcac
    21241 agtcccttat gttttgcaaa aaaagttttt cttttttctc gtaaattcgg taaaatcaaa
    21301 agaaaaaata gacaatagaa aagagaagct tatgtgggaa aaagtagaga aaaaacaagg
    21361 accgatttat cgtcaaatta tggaacagat tatgagagaa atcgaaaacg gaaggatcaa
    21421 tcctggaagt caacttgaat cagaacgaaa actagggcaa ctttttggtg tgaatcgttc
    21481 caccgttgtc catgcattgg aagagcttag ggagcttggg gtactaacga gcaaacgtgg
    21541 cagcggatgg tttgttaatc agactgaatg ggggaaattc gctgttccaa gaatcgattg
    21601 gcggcaaatg atctctcctc gttatgagca gacggactgg tatgagcaaa aaataagaga
    21661 agcaaagcag atccaaaaaa catcgtttct tgatttatac gctagcgaga tgccagatga
    21721 gcttttgccg aattttgagt ttccttctta ttctcttgaa gaaatcttag cggaagaaaa
    21781 agaaatggat cttttaggct atactccgtt acgtaacaaa atcaaacggt atttggaaag
    21841 taaatttgaa ttggcatttt cccccaacca gcttcttgtg actggtggcg gtcagcaagc
    21901 aatctttttg attttacaaa cgattctttc agctggagag gggatagctg tagagtcacc
    21961 ttcttttttt taccgattac ctcttttcaa agcttcagga acgcgtttat ttgggatacc
    22021 gatggatgaa gaagggatcg acttagaaaa actgaaagaa tcgatccata aaaataaatt
    22081 gaaagcagtg cttatcaacc cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa
    22141 acgacgagaa aagctagtag aattgtgtcg acagtatcgg cttccaatta ttgaagacga
    22201 tgtatttacg gatctttcat ttctaaacca agaagaagtc cttcctatac gaacgattga
    22261 tccggataat gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat
    22321 cggttgggtg ataggacctg agacttttat ctcgcaacta gctcaagcgc aagaaatgat
    22381 ggagttttcg atgaatatcc ttacacaaat cacagtggct aatgtattta atgaagcaat
    22441 tgatttgaaa atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc
    22501 ttggggagat tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt actatgcctg
    22561 gattacttgg gatgggaaaa aactagaccc cgaactagca gaaaaagtag tagatatggg
    22621 tcttggaatt gcaccaagtc tactttttgg gcaagatata aatggaatca gaatcaatta
    22681 cagtaggctg gatgaaaacc aaataccgtt gttttattca aagatgaaaa ttctagaaaa
    22741 gtggttaaca gaataatttc acaaagtaaa tagattaaag aatataacag cttggattta
    22801 aatttcaagc tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa
    22861 accattattt gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg
    22921 tataacacca acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa
    22981 aaatgcagat gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa
    23041 tatgcggatt ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct
    23101 cataaatcca tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact
    23161 gtttcaatcg cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg
    23221 caaacaaaaa agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc
    23281 cagtatatca agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt
    23341 tatccgatga tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc
    23401 aacgttcaac agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac
    23461 aagaggcaag tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag
    23521 gttatcggga tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat
    23581 ggtctcgagg acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac
    23641 aaattatcat cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac
    23701 gaaacaagaa aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt
    23761 tttacgaagc aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa
    23821 acaacgggta tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac
    23881 aaaaagctgt cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag
    23941 tatatactgt atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa
    24001 tcgaaacact gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct
    24061 accgtacaaa agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta
    24121 aatatttgac ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa
    24181 ctaaagaaga aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt
    24241 cagatttctt gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa
    24301 tttatgaagc gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac
    24361 agatgcctta gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt
    24421 tgggaaaaac ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga
    24481 ccgagtattc gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc
    24541 gttagaagga tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat
    24601 ggacgaagta gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct
    24661 acccatcaca attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga
    24721 taatttagaa ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc
    24781 ttatgatgca aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct
    24841 agaacacatg aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt
    24901 tcctttggat aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg
    24961 agcaatggtg cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt
    25021 tgaaatcatt gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt
    25081 ggaaaaaact ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc
    25141 gcaagtagtt tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg
    25201 agtttctgca cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa
    25261 tgcaaaagat atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg
    25321 aaacaaagtc atggcttatc aatttaaatt
//
LOCUS       DACCCR010000033        24747 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCCR010000033 DACCCR010000000
VERSION     DACCCR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.034"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(57..173)
                     /locus_tag="IVK93_001420"
     CDS             complement(57..173)
                     /locus_tag="IVK93_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP6386716.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(418..1332)
                     /locus_tag="IVK93_001421"
     CDS             complement(418..1332)
                     /locus_tag="IVK93_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6386717.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(1347..2348)
                     /locus_tag="IVK93_001422"
     CDS             complement(1347..2348)
                     /locus_tag="IVK93_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6386718.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            complement(2345..4528)
                     /locus_tag="IVK93_001423"
     CDS             complement(2345..4528)
                     /locus_tag="IVK93_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6386719.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            complement(4531..6978)
                     /locus_tag="IVK93_001424"
     CDS             complement(4531..6978)
                     /locus_tag="IVK93_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6386720.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(6962..7354)
                     /locus_tag="IVK93_001425"
     CDS             complement(6962..7354)
                     /locus_tag="IVK93_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6386721.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            complement(7441..7938)
                     /locus_tag="IVK93_001426"
     CDS             complement(7441..7938)
                     /locus_tag="IVK93_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6386722.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(8055..8279)
                     /locus_tag="IVK93_001427"
     CDS             complement(8055..8279)
                     /locus_tag="IVK93_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386723.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="IVK93_001428"
     CDS             complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="IVK93_001428"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6386724.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11077..12282)
                     /locus_tag="IVK93_001429"
     CDS             complement(11077..12282)
                     /locus_tag="IVK93_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6386725.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(12305..12458)
                     /locus_tag="IVK93_001430"
                     /pseudo
     CDS             complement(12305..12458)
                     /locus_tag="IVK93_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(12461..13846)
                     /locus_tag="IVK93_001431"
     CDS             complement(12461..13846)
                     /locus_tag="IVK93_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP6386726.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(13875..14261)
                     /locus_tag="IVK93_001432"
     CDS             complement(13875..14261)
                     /locus_tag="IVK93_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6386727.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(14277..14591)
                     /locus_tag="IVK93_001433"
     CDS             complement(14277..14591)
                     /locus_tag="IVK93_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6386728.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="IVK93_001434"
     CDS             complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="IVK93_001434"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP6386729.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15851..17140
                     /locus_tag="IVK93_001435"
     CDS             15851..17140
                     /locus_tag="IVK93_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6386730.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(17179..17784)
                     /locus_tag="IVK93_001436"
     CDS             complement(17179..17784)
                     /locus_tag="IVK93_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP6386731.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(17980..18444)
                     /locus_tag="IVK93_001437"
     CDS             complement(17980..18444)
                     /locus_tag="IVK93_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6386732.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(18466..18786)
                     /locus_tag="IVK93_001438"
     CDS             complement(18466..18786)
                     /locus_tag="IVK93_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6386733.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="IVK93_001439"
     CDS             complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="IVK93_001439"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP6386734.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20208..20558
                     /locus_tag="IVK93_001440"
     CDS             20208..20558
                     /locus_tag="IVK93_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6386735.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            20743..21696
                     /locus_tag="IVK93_001441"
     CDS             20743..21696
                     /locus_tag="IVK93_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6386736.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            21709..23496
                     /locus_tag="IVK93_001442"
     CDS             21709..23496
                     /locus_tag="IVK93_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386737.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            23483..24685
                     /locus_tag="IVK93_001443"
     CDS             23483..24685
                     /locus_tag="IVK93_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386738.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         6992 a         5007 c         4518 g         8230 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggtg caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
      421 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
      481 cgttgctttg gtctgctggt cgatatactc cactgtcagc gataccgtga cttgattatc
      541 cttacgattg taaataggat ttaccagttc ttgaaagatg tactcttttc cgattggttt
      601 taatatcccg tcattcacat agtaggaaag ttcactggct gtcgctgtag gatagagctt
      661 gaagaacgtc gttaaaaact cattgatttc attggttgta atggaatcaa ccgtaccctc
      721 actttcaata gcttttggtt tatagtctga tttctttggt atgctagtaa tggtcggatt
      781 cttaatgagg accatatttc cagtactgtc tacatagaca ctcactatat aagcagagtg
      841 gacggtcttt gtattttccc cctctgtaat gagttggtct acactgtagg ttacatcaaa
      901 ttgattgtcg ccatctgctt ctaccgtcca tatctgaaat ccttttacag aagacgatac
      961 aggaatatct ttacgaactg tatcgacatt cagagcttga agttcctctg tcagatatgc
     1021 tttcaggttt tctatgcgat tatcaatgga cttgtcgttt agttcccatg aatagtagac
     1081 tttcgcaaag ttctctacaa aattttccac atgatgagta tcgacatatt ccttttctat
     1141 gatggtggtt tcatgaatgg tatgggtatc aatggctgta aagtgcttga atatcgcaaa
     1201 gctgaaacta agtcctaaaa gtacccatag gacaatcaca acctttttac gaggattgac
     1261 cttatagaca cgaggtttct tttcctttgg tgtctgtttt tctgtattct gattttttcc
     1321 gaatttcatc attgaatcgt cctttcttat tgtttgattc gtcctgctcc cactaaatgc
     1381 tgttgccagt aagagcttgt taagtcggca taaccgatag ggtcgcctgc atgaaacata
     1441 cggttatcgc ctaaataaat cccgatatga gtaatataag agccagcgtt ataggtagaa
     1501 tgaaagaaaa ccaaatcgcc ggcttttgct tccaataatg ggatatgccg ggttacatca
     1561 tattgctgtt gtgctgttcg tggtaagttg attccagctt ttccatacgt ccattgtgtc
     1621 agtccgctac aatcaaaaga ggtagtcggg gaagctccgc catagacata cttccagcct
     1681 tcaaatttca gagcttcgtc catgatggct tgtactgtat catcatcaaa ctctgttgtg
     1741 acaagatact gcgttaccag ttgcacataa aacatattcc cgtaattgta tcgccagccc
     1801 ccattgatag gtatggctat gggattgggg taagacactt tttcgtcacc cgaatactct
     1861 tttgagaaac tttgagccag ttcaaaggta tatttattcc cacgattagc cacatatcct
     1921 aagaaaccac caccataatt gtaggactgg ataaccgatt ctaaatctac accaagcctt
     1981 tcgctactgg ctaataactc actgaaatac ttcacacctt gcttaatgga ttcttctgta
     2041 ctcaatgaat taggtggaag accgagggat tccgaggact gcataacatc ttctgctgta
     2101 ccacccgatt ccacctgtat aatcgcaaga agtatattga cgtattcttc aattccatat
     2161 tctttggcaa ctttttctac cataggctta tgagccagca cttctgcgga aacatccaca
     2221 ccgccatagt gaatgttgga aaaaccgcca tcttgttcat ctgaaaataa aatggcgaca
     2281 aacagaagca gtgagaagac catcaagaat aatcccgaac caccaatcac taaagttttc
     2341 agcttcatgg tttcttactg cctttcttaa tggtggcggt tttgattggt ggtctacttt
     2401 ttgtattttg tagtggtact ctttgaacag tagactgacg ttcttttgtg attgaacgtt
     2461 gtgaagctct atctgttggt gcagttgaag ttactggctt ttgaaccgtt ctttcttgaa
     2521 ctgtattact ttgacgttcc gtttttaaac ttgaagaatc ggacttaact gttggacgct
     2581 cttgtttggc ttgttgagat tccctatagg aagcctgtac agttgtttgc cttgaagtct
     2641 gtccatcatg agattgttct tgcttagtgg ctggtctttc atgaacggaa gaagctggct
     2701 gttttttctg tttgacctgt tccatttcag agcgacgctc cgcaatggtt ttccgtcttt
     2761 gttcctgctg tttcttgcgt ccactggctt tatccgcttt ggtttgagaa atacttctgg
     2821 ttaaatcatg gacattatcc gtcactttag attttccttg atatactgca tatcttgcat
     2881 tggtcggcaa atccttaacc tgttctttca aattaccagc agtatctacc attctgtctt
     2941 tggtatcagc tactgtaccg atggtttgac cgatacgctt tcctagtgtt gatttttcct
     3001 gcccgtttgg tcgagagtga tctgcttgtg tactcgcaga acttcccgaa cccgactgtc
     3061 cttttttacc cgtagcactg gcaatggcag acccagcccc taaagcagtc acggagcgtc
     3121 caagtttccg ctgtagacgg tgcatgtgag cgtgcataag catacgaggt tttctcatca
     3181 tacgacttcc cacactttga gaatcgttac tctgtagaga aaacatactc attaaatcgc
     3241 ccagcttaaa gtagattcct gcaaaggtca caatctgtag aaaagcaatc aaaaagaacg
     3301 gataaccagc cgataaggta tagagcatgg ttgaaatact aaatgctgtc gtaataatca
     3361 atgtgattcc agctcgtgtc aaaatggtat taaagagctt tgttatggct cgttttgaca
     3421 taccatcaaa tgatggaatc atgcttaaaa taaagctcac aggcagaaac atagcataga
     3481 tgataaaaag tacctgcgag aaaatcatga ttcctgttaa taggaataca aatatggaaa
     3541 tcccaatatt gaagacaaat aggaagaaga ctgtacctaa acggttaatc gtctttgtaa
     3601 tcgtcatatt ggtattgctt ctatcttcaa tttcttccgc aacaattttt tctctgtctt
     3661 ccccattgtt ggaatctgga ctggttgaga gcaggctttc cacacggtca ataccaatac
     3721 tttcaatgtc tgaactgtta tattgaagca gtagccacgg ttgctgaacc tgtatggaaa
     3781 ataggctgtc acgtatcaag tccacactgt ccttgccttg actatctgaa tggggcatga
     3841 caatttttgt acccagtgac aaactagcgt tgctgatgtc tgatgaaaag tcattgattt
     3901 ttttaatgta gtcgggagcg taggcaataa aggaagccga taggataaaa accagcacaa
     3961 aattcatgag ggcatgaatt gcctttgtgg tttctctctt tatcagtcct gtataggcaa
     4021 cataaacccc aagaaccaaa atcaagagta aaaggaatcc aacatagaaa ccctctgttg
     4081 atagtccact aggcgtaacc cctgcaaggg tctgcatatt cttgccaatg gaatctgctg
     4141 tagcggaaat gaagtctaag gaataggctt cctgtactaa gtaacccgtc gcattggaaa
     4201 cataaagact gattgtccaa ataaaattgg taatggcata tagtccatac atgacttgtt
     4261 ttccaattcc gtccgaccag ttccacggta gccagcccca gctattatcc acataaaaat
     4321 ccagttgata gttttcaagt gggtatcggc tgtattcatt tgccacattg accgtatcat
     4381 ctaccaagcc cgcagcttga accaccgttc ccagcatggc taaaagaaaa atggcaatca
     4441 caagtgtgaa agccactgtc attgccactt tacctagacg tttcagcgtc cagtttgatt
     4501 ttattctgtt taatattgat ggtttcacat ttacacctct tttcgcacag gtggtctggt
     4561 atcaaaggca tggagcagtt cttcaaatac agggtggaac tgtatcacac cgacacgacc
     4621 atataaatca ctgataaggc attgcccgtt ttccaaatca cgcaatcgct tctgattgtt
     4681 ttcgtcctct gggtctacac caaaaaaggc aagggttttt ttaatctcgt taaggtcagt
     4741 ggaacgaaag gcaaatttta agccgaggtt atttttcagt ttttcatcta agaggtcatc
     4801 tgtattttgg gtcacgaaat ataccccagc gttcatagca cgaccagcac gaaccagctt
     4861 catagatagt gttttccctt gtgccacctg taagaagctc catgcttcgt ctaagtctac
     4921 aatcttgaaa atacttcggt ctgtatggat aaagtccaac gcaaaggtac taatgacaat
     4981 cagcattgct accgatagta actccattgt ggtatattcc tcaaaggaag tttccttgtc
     5041 aggaagtacc aagtcggcaa cctgtataat gttcagttgt ttttcaaggc tgatagactg
     5101 ctctacataa ccatcactaa aaagcagatg ggcaaagtca tagtctgtaa aactttcgat
     5161 atggtcggct atgctggtac ttagtggcgt attctcaacc cgtaattctt caatcacttt
     5221 catcaaccct cgcacttcac tattggttac tgcacgaatg gcttttctaa ggattgggaa
     5281 gcgttcccca tcacgagagg aaatccccgt aaggaatgtc aaaatatcaa tagccagtga
     5341 ttcagaatct ttggtatttt tcataattac ataagggtca agtaagcctt tgtttttctc
     5401 atcagaagtc agattgacga tattgatttc atgggaaatc tctggcaagg tttctttcca
     5461 tctgccacgt tctgcttttg ggtctacaat cactgcttgt gccccataaa gcactgcata
     5521 atagacgata aggttattcg caaaggattt accaccaccc agcgaaccga caaaggcaga
     5581 cgctaacgca ttggttactg aacccttaac cccttgactg gcaagagcag gtttcagata
     5641 gacattgcgt ccagtatcta agctgtagcc aacataaatc ccctcatttt cacccagcat
     5701 ttgagtagca ccaaaaccta aaccagcgag gaaatcagag gtcacgtatt gaatatagtc
     5761 attcatatat cttttgctgg caggtaaaaa ttcttcatgt aagcccagca tatccccaaa
     5821 aggtcgtacc agtttcacgc tcaaatcatc atagaaatct ttcacttcat tacaacgacg
     5881 tttaagttcg tcaaggtcat ttgctgatac ccttacaaca taggacagct tatacataga
     5941 ttccttgctt tggtctaaat tggtttccag ctcgttcaca ctttccagag cttccgccac
     6001 attagagctg gtttcattat cactttgcca agcgtggtta tccaagtctt tcagttcttt
     6061 ctttttattg cggacagtag atagggcttt acgattcgct acaatttcca cattcattga
     6121 cgtatcaatc ggaaaggtaa attgctgttg ctggtagtag aagatttcag aggacgggaa
     6181 gtccagttcg ccgacaatac tgttgatggt aaagtaagct acataggcgg tttcgtcttc
     6241 ctgctggatt ttcaaatatc gctgtttttc ttccaccaga cagcgagtag gcttaatgag
     6301 gtcataggtt ttaatcagtg tttcatgttc cagctttttc tttgatagag gatactcata
     6361 ctcttcatag gcagtgcctg tctgtccgta aaggtgttca atcagatagc cgaagtcatc
     6421 cttatctaat cttcggattt tgaaacgacg ggagatttta ttttctaaaa gcttttccat
     6481 cttctgaaaa cgcaggattt catcattact catactaaca aaatcgccca tcagcttatg
     6541 gttcacatca tagacaaaat cagtaaaagc attttttgct tcaacggtaa gacttttcat
     6601 agaaaactcc tgatcgttga gaagcaactt aaaaccgata aagaaacggt agtccacttg
     6661 attttcgcca atcatggata ttaaagcgtc tgtctgttgg tcgattttgt cataggcaac
     6721 cgttttgagc tttccagtga cttcattttt ggaacgttct tgtgcagaaa gtatgctgga
     6781 ttctgtactg atttgtaaag catgaatctt gccatcacga ttttgtgcga taagctgtct
     6841 gaaagaatca tgtacttgta ttttctgttc cggacttaga aatgagtaat tgtagggaac
     6901 aagctcatag taagcgtaac attccccatc tttattccag accagattgt tttcaatata
     6961 tttaattgga tatgccataa aattcactcc taactgctgt aatggcttct tgtggctggt
     7021 ttctgccaag cgttactttt tttcctgcat aggtcagctt tcgtcgcagt gcataagcaa
     7081 tgacagactt caaaaatcca taaggctttt taccatcaaa ggtttttgta gacataaacc
     7141 atgtgaaagc cacaggaatc ccaaagtatt tgagaaatgc tccctctatc atagaaagag
     7201 ggggcaagtt gccaagtatc ataactgcaa agagtgacac gacaaaccat gtcatttgcg
     7261 taaaggttat gggaaacgga agtctaaagt cattgatgga gtacaacact ttctcaacag
     7321 accagatact ggtatagctt cgtattttct tcatgtaatc aatcctttca taaaaatggg
     7381 gatagtcttt tgactacccc gtaaacaaga aaatctgcca gtaagaatgt accgacagat
     7441 ttaatagacg atttcaaaaa tcccgtgatt ggttgagata aacgttcccg aaaggtctaa
     7501 atcccgacca taggattcat aatcaatata gttttgaaga ctggctggca cttcgcctaa
     7561 agcacccgtt tcttcaatgt agtagcgtgc cacgtcagac atatcatcac aatcggaatg
     7621 aatgataata tcttcttgat gttcgcttag ttcttcaata cttgaaaaat gagtgagcag
     7681 agcagatagc tccgattgta gttcttctgg caactccgat accatttccc ataggcgatt
     7741 gagttcgcta atagaagtgt attcgtcaac cgtaaagggt aactcgtaat catgaatggc
     7801 gtattcctca tattcatcat tcaagccgat tttctctttg acttcctcaa agtcaatggg
     7861 aaaggtaaac cacgcaccga ccaattcgcc ctcattgtat ttgcctaaat tcgcaatata
     7921 gacttgcata tcgtccatat attcacgtcc tttctttgta gagattcaaa aatccctacc
     7981 gcacttcgtt tggtgtacca ttcctttgcg gaacataaga aaaccactta tattccacaa
     8041 aagaacggtt ttatttaagc accaataatg cgattgaata gctctagtaa aatgtctttt
     8101 actccagcag cgttgaagac taagccaacc gcaataatcg caataattaa aaagccaatc
     8161 agtttgctaa actcacgctt gaagccaaga tacaagccaa tcacaacgat tgctaaaagc
     8221 accagtgatt gagcgtttga tagaaaccag ttatatatgg ataggtaagt tcctgatatt
     8281 ataaatatct caaacttttc cccccattca cacggagcgt gcgactttca ccgcactccg
     8341 cgttccatcg tgtttatatt gaaaagtatt tatgtagtag acctatatca agtttaattc
     8401 ttgattgcca gctaatactc tatatttatt tagtcttatg agctggactt tatttagctt
     8461 caatagagct atatattttt gtattgtttc ttctgtttta gcgtgtatta gacggtgtac
     8521 cgcctctttt accaaaataa gatttttata atcgtcattt ccaccagctc cagttggtac
     8581 aatatgatga caatgtattt cattagcttg ttgaaattct tccagagtta tagcacattt
     8641 tccatgttga gcagaaaata gcgaaattcg attgtctgcg tattctattg tgcgattttc
     8701 caaagataat ttcattaact ggtacatcag taatttatca acacttagat tggtgtggat
     8761 aaattttctt ccctctagag tgtagcaatt cgctaaatac acctttgcca ttggttttct
     8821 gtgttggata tatccaacag ggaaaatagg ttcgccctcg ccagcaacat accgaagcat
     8881 tttagattta ccatatcttg ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa
     8941 tctgcccctt ttgtttgtac ctaatctgtt agtgaagata gccattacag ctctattcag
     9001 tttcatacaa tccagattta cattcgttgc aatctgataa taattttgca tacctaatac
     9061 cattgaatta taaagtcttg tttcttctaa ttccgttttc ccttttgagg gagaagcaat
     9121 tttctttgcc tgtgatacca gcttgtcttt ttggtttttg aattgtttat cacttatatg
     9181 tgatttaaca actaacttct ttcttctcgg tatcatttta atcttgaaac caagaaagtc
     9241 ggagtatctt tttctaacat ttacaattct tgttttttct tgtgatactt ccaattttaa
     9301 tcgttgttca atccattgag tgattgctat tttggctttt tcagcacttt ccttgtagcg
     9361 gcagaaaact ctaaaatcat ctgcataacg aaccatatac atttccttta actttgtttt
     9421 cttcatttct ttgtaagcgt tgcttttaca tgggctaccg cttttattga tatgaaccac
     9481 atagtttttt gtaacaggat tttcctgcca ttggctttct acccaatggt ctaactcatt
     9541 aagaacaatg tttgctagta gtggggatat tattccccct tgcggagttc ctttctctgg
     9601 atatgttatt gtaccgttag tcattttgat tggagctttt aatatccttt ttaaaatgaa
     9661 tatcaagtgc ttgtcccgta ttcccatagc ccatatctgt tttatcagct tggaatggtt
     9721 cacgttgtca aagaaaccct taatatcaaa ttcaatcaca taatgtagct ggctgatttg
     9781 taggcggtta taggttgctt ttattgcatt ttcaactgac ctattaggtc taaaaccata
     9841 gctgttttca ctaaatttag cttcgcagat tggttctaat acttgtttta tacattgttg
     9901 tataagcctg tcccacatac aaggaatacc tagaggtctg gttttaccat ttggttttgg
     9961 tatttctttt cgcctcactg gcttaggtcg atacccatgt ggactgcctg caacaatgta
    10021 tcttactttt ttgacaactg tttctgctgg caatttgcca atatctttaa ttattgtacc
    10081 gtctgttcct gctgttttac tgcctgcatt tgctttgata tttcgatatg ccagtaatat
    10141 attttctctc gataggataa tatccataag atgagtgaaa atatctccat tcttactttt
    10201 ggcatataaa tcatcaaagg tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc
    10261 tacacacaat gtttcatttt tatttttagg cataaggctt cactccttta cttgaaattg
    10321 agtgagcttt tttatcttac tcgtaatcct tattgaaaca acataatact tttctaatat
    10381 atacacgact tgagggcatt cctccattct cattacaaga atttcttagg ttcgccctct
    10441 gcttttcagc attagttcaa ctgcttattg ttcttcgtca gtttattctc ttgatgagaa
    10501 cccaatacct ttcctcgttc cgataatctt atctatacat atatatcctt aggtcactag
    10561 ctataagcct gctggcttga taccgcctgt aacgttatat ggcatttcat aacctatatt
    10621 cttactttgc cacaccagct acgatttctc gtacctttgc atttctacaa agttcaaatt
    10681 tagacccgta cattcgctgt ttcgtcagtt ccttatagaa cattctaacc atagatattt
    10741 tatagacccc cgacctgttg gatacgctac ccacctctgg atagttttcg atagaagata
    10801 tatgttcatc aactattacg gtatctttca gccgtcttta ccgagcttca aacattctga
    10861 gtttcctcat acaatgcttg tcggaggctc agggcaggcg tttcagagcg ttacttcatc
    10921 attccacctg tcagattatc agttctccta ataaccaaac tttttcgttc ttttattagt
    10981 gccttagctt ttcactaagg aacgagtcgc acaaaggttt tgtccaaaat tcataaaaat
    11041 gttctcctct ctatattcaa tgaatttgta tttgagttat ttttttgttg ttatcacgtc
    11101 ctgttctttt actgactgtt gcttcaaaat ctgcttgtgt cggtctgtca gtttcgcatg
    11161 gtcgagaatg tcttttacaa cctgcgtctg gttgatttca tcaagtttaa tcgcaacctt
    11221 taaggtcggg gcaacttgat gagatagcca gttcagcgtc ctttggaagg agtaaggctc
    11281 tggttttgtg gttagtttta atcgttcacg attgttccca ataaaccaag cccattcttc
    11341 attcagtttc caatcagaac gaggtttgga atcgtcttta tctacaaaac ggatataccg
    11401 attgataatt ttaaaggcgg tatgctctgg attgtcatag acgagtaaat cacggactgc
    11461 ataataggca cgctcatttt tcaatcgaat ctcaaaacgg ttttttactt ctgcgtcttc
    11521 aatgggaata tcatttttct tgtactgctc gtagtccttt tcatagatac agaaataaac
    11581 ttcactttgt aatgaaccga tatagagggt gtttcccata cattcctttt cctctttgcg
    11641 taccagttcg ccactgcgat agcttttaaa actgcggaag acggagatac attcttcctg
    11701 ttggcacttt tcagtgagta cagggatatt taaaatccct gtcttatcgt taatggcaag
    11761 gtcaaggcgt ttcatcacac cgccagccac caaaacgtcc ataaagaact cataccagct
    11821 tctttgttgt gccagaagat agctttcaaa ttgtctgcac ccacgacctt tcaattccac
    11881 cagaactcct ttgtccagtt catgggagca aaggacgaat atgtcgccta aagcataatg
    11941 ctctgaataa gaatagaaac catagtcctc atgaagaaaa taggacagtt tcagttgtaa
    12001 gatgttttcg accacctgct gtacgtctgt tgtcggaaag cgaattctta cataatcaaa
    12061 cagcatttca aggggagcgt cgggattgaa gcgttccaga gcttcccaaa gggactgctg
    12121 taaatcctct gatggcttga cttttcctgt ttcaatatcg cttagatact gccttgtaat
    12181 accagtcgca acagctaaac ggttttgaga tagtccataa gccaagcgtt tttcttttaa
    12241 atgctgtaac caagtttgtt cattcagtaa aaatccctcc aatcaaaaag gcgtatgtca
    12301 acttttaaag cccatttgac atacgctgaa attttgtaaa tcccttgtaa ccaaaggatt
    12361 ttctaatgtt tttttgactg tttcctgtcg atttgtaccc ccctgttaga taccgagggg
    12421 ttaagtgctg gcgtggctat tgccacacca gccagcaaga tcagtccaca cctgcgactt
    12481 ccgcttcgca cgtcgcctgc gtggactgtc tgctgttgga taacttttta atttcctcca
    12541 agaaatcata tccttttggt acaaggggag tataaaactc tgatatgaca cttgttccta
    12601 catcaacata gccacgacct ttgattcgct ttaagaagaa atccttttgt acgtcactgc
    12661 caaacatcat gccatagccc atttcagaca tacgacctaa agccactctg aaattaaact
    12721 gatcacggat tccgtcgcct aaatattttg cgtctggacg ttgacaagcc agtattagaa
    12781 agaagccagc ttgacgacct aacatgacaa tctgtttcag cttattcata actgcggtgt
    12841 tttcttttgt tcccagcatt tccatgaaag cgacgtattc atcaaagatt aagaagtgtg
    12901 ccgggagacc taagtaagca taatttttgc cagtcttata gttcttcatc tgcttcattt
    12961 cctcactacg tttcatcatt tcttcataga atgtttcaat gcaagaaagc aagtcttctt
    13021 ttctatagta gacatttgcc atcacagaac ctaagtccgc aaggtcagca tttttcgggt
    13081 caagaatata cagttttgaa tctgtatgaa gcaaggcttc aatcagtgtc agtataaagt
    13141 aagttttacc gccacctgta ccaccagcaa tcaacatatg agggagctta tcatattccc
    13201 accatacgtt tttcattaag cgaagtttac catctttagc ttctacttca tcaatagaaa
    13261 tacgactggc tatggtgtca tagagcaaag tatattccac ataggaatcc tttaactctt
    13321 tatccgtcag ctcacagtac aagccactct ctaatttctt ttccaagtgt aagagttggt
    13381 cttgatattt tcccagcgtg atttccaccc gtatctgtat caagccattt ttaagtcgat
    13441 aatacatttt agggaagtag gttatctttt cctttgtacg accagcacta tctttaaaga
    13501 aaccctctgt tttgacctgt tcagattcat accacttgtt ttcaagtatc atctttgcca
    13561 gtttttgacg gtggtaaagt tgtttaaccg tatcatagcg aacccgtttg aatacaaacg
    13621 ctaccagcaa gcagataaga attgcgacac tgaaactgat aattaaatag ggaatgtcaa
    13681 tcttatctgc ttgtgatagg ttaaaatcct gccagttgat ctgctggatt gtcttcacat
    13741 gaaacagtcc gacaaccagc aggaaaacag gcaggagtga cgctatcgta aaatgaaaga
    13801 ctaaatcttt accagatggg cgaatccttt taccacgctg tttcatgcga aaaagtctcc
    13861 tttctaccta gcgactattt gtcttgtgtc ggttctttct ttgcttgtgg ttgagctttg
    13921 aatgaactag aatcctttgt cagcacaata tcgtctgcct tgatatacca gtcaacatct
    13981 gctccttgat aggtggcagt agcaacggtg tccgcaatgg gattgataag ttccacccgt
    14041 gcgttataat caaactcttt caaaggcacg ctggcaggaa tacttacttg aatcatgcgt
    14101 ccttgtcctt tggattttaa gtcataggta cgttccttga tttcatctga aaccgacccg
    14161 tcttcatttt ggattctcac ttcacgacgt agagcagaga atttcaattc tccaaaagtc
    14221 gtgtctttat ctaatacaat gccatttgct aatctcatca tttttcctct ctttctttat
    14281 tcttttatca tgtcgtcagc atgtaaaagg taatttgtaa aaccacgagt gccgattttg
    14341 tagccctctg cggtaatacg tggattgact aacttcacac gttcctcaaa gccgaaatgt
    14401 ttttcgccag cttcagcagg aagcaccacc acaatatcat ctgctctttg aacatcagaa
    14461 tagagattat agcttcttga taagacagtt agccgtccgt tgattcttcg ctgaacgact
    14521 ttatcctcgc cagcaaattc taaattgccg aatgtttttt ccatgttggg aatcacaaat
    14581 ttaagttcca tatttttacc tatcctttct tttttattgg ctgaatgaat gtttgatggt
    14641 cttaaagagt ggggaacgac cttttgattc ttgatttttt gttttcataa gttcacttcc
    14701 tttcaaaatc gggtaaaaaa atagacacct cattttttga agtgtctacc tattaaatat
    14761 tcaaatttta ttggaagtat ctttatatct tcacttttca aggataaatc gtcgtatcaa
    14821 agctcattca taagtagtaa attagtagta aattgagtgg ttttgacctt gataaagtgt
    14881 gataagtcca gtttttatgc ggataactag atttttatgc tatttttctc ttaaaaaata
    14941 tcattatttg tggtatggag cccccgcgtt gatccggaag gcacggtaga tttgctcgac
    15001 gaggatcagg cgcatcaatt gatgtggata cgtcattttg ccaaaagaga tctgagcatt
    15061 gcttcttttc atgacggctt cgctgagtcc taaagaaccg ccgataacaa aaacaaggtg
    15121 gctttttcct tggataccta attggtcgat ttgtttagca aaagattcgc ttgtgggatt
    15181 tttcccttca attgctaagc catagacgta ttcttgttct ttgattttcg ctaagatccg
    15241 ttctccttct ttttctttta cttgtctcat ttgcgcttcg cttaaattct ccggggcttt
    15301 ttcgtcagga acttcgatca attcgatttt cgcataggca ttcagccgtt tgacatactc
    15361 attgatacct tggatcaagt atttttcttt taattttcct actgaaataa ttttgatatt
    15421 catgttttct ctcctttgat cttcacgctc actttgttta ttataatcaa ttttttcgtc
    15481 tatatgttta ttttgactat ttttgttaaa aagatttccc caacagttat gcacaacatc
    15541 cacaggttta tccacagaaa acagcgttgt tttaccttat ttcatcacat tcattaggca
    15601 aacatacgtt ttataagaat ttatccactt tatacacagg ctgtggatat gttttatccc
    15661 ttatttttcc aatcctatcc gcgtagtttt acacaatatc tgtggaaaaa gaaaatcaaa
    15721 tattttcatc tgaaaacaat gatttttcct aaagtttttc ataaaaagtt caaagtctca
    15781 aagtagacta aagtcaacaa ataagactat actaacagta gataaatgaa tgtaattgga
    15841 ggaggaacac atggatagaa aaaatgtgac accaaagatg aaaaaaaata aaaataatag
    15901 cctctggcgt aagttgggtc tggggcttgt tggagggatc gttggaggac tactgacagc
    15961 tggcattttt tatgcagcga tgggcactgg caacaatagt tctactgcta caagcggcaa
    16021 tcaaaattct gccggtgaaa cagttgtgga aaatgtcaaa gtcaatgtag attctgatat
    16081 caccaaagcg gtagataaag ttcaagacgc tgtcgtttct gttatcaact tacaaagtca
    16141 aaaccaatca agcggttttg gcggattatt cgggcagcaa gaagaaagta gcagctcaga
    16201 tgatagtaat ttggaagctt atagtgaagg aagcggcgta atctacaaaa aagacgggaa
    16261 tactgcttat gtagtcacaa acaatcacgt agtagatgga cagcaaggtt tggaagtcat
    16321 gatgaaagac ggtacgaagg taaaagctga attagttgga acagatgcct attcagattt
    16381 agcggtgttg aagatcaatt cagacaaagt cgaaacggtt gcctcttttg gggactcaag
    16441 tgctttgaag gttggtgaac ctgcgattgc gattggttcc ccattaggat ctgaatatgc
    16501 gaattctgtc acttcaggaa tcatttcttc cttgaaccga caagtaacaa gtacgaatga
    16561 atcgaacgaa acagtcaata tcaatgcgat ccaaacagat gcagcgatca acccgggaaa
    16621 ttctggtggt cctttagtga atatcgaagg acaagttatc ggtatcaact caagtaagat
    16681 cgcaagtact tctgaatcga cttctaatgt aagcgtagaa ggaatgggat ttgccatccc
    16741 aagtaacgat gtagtgaata tcatcaacca actggaaaaa gacggaaaag tgacacgtcc
    16801 agctctaggt atcacaatgg tcgacttatc tgccgtttct actcagcaac aagaacaaat
    16861 cttgaagatt ccttcatctg taactaacgg tgtcatcgtg acaaatgtcc aaacagctac
    16921 accggcagaa aaagctgggt tgaaacaata cgacgtcatt actaagatcg atgacaaaga
    16981 agtttcttct ggtgtagaac ttcaatctgc tttgtataag aaaaaagtgg gagattctgt
    17041 caaaatcact tactatgaag gctcgaaaga aaaaacaact actgttgaat tgacgatcga
    17101 tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa taaaaaagag gttgtgacaa
    17161 agacttgtca caacctcttt attttggaaa tacttctccg actacggcat cctcaggcaa
    17221 tactaagatt cctttttttt ctggagcatt tggcaaatgc aattcacgag cagaacaaat
    17281 cattccatag ctggctaccc ctcgcaaagt tcctggccag atcatcattc catctggcat
    17341 catggctcct ggttttgcaa cgacaacttt ttgtccagct tcgatatttg gtgcaccgca
    17401 aacaatttgc aatactgtcc cattgtccac ttctgtctca gtaatcgata aatggtcaga
    17461 gtcaggatgc gggacgcatg tttttacata cccgacaacg atcttcggtt catgatcagc
    17521 ttcaagctgt tcttcgaatc ctgcttcttt aatcaatcga ttcaatttcg ctaattcttc
    17581 atctgttaag cttacttggc cgttcccttc aattgttaga tgatcggaga tatggaaaaa
    17641 gttccaagca acaacttgac cttgttcatc tttgatccga gcaacattcc atttacgttc
    17701 gacagtgttt tctgttcctt tatcgtcggc tatgataacc atcaaagtgt ctccgacgtg
    17761 tgctttgtta taagcaaaaa tcatgttctt tcctccatta tcctttgctg agatttatct
    17821 taacaaaaaa ttgcattcac tgcatgagat ttttttcatg ctttatttcc gaagaagctt
    17881 cttctggaac accatttatt cgtaacaagg gactgtgaca agacttttgt cacagtccct
    17941 ttactgtttg attaaaaagg ttatacaagt tccacttgct taattactcg gctgttcttc
    18001 ttcgtcagaa acgatcaaga catcacaagg tgaatgacgt atgacgtagc tggctacact
    18061 tcctgtcata aagcgttcga ctgcatttag tccagactgc ccaaccatga ttagatcgat
    18121 tgcatatttt tttggcaatt tgactgccat ggattcacga acagagccgt atgcaataat
    18181 tccttctata tttgtgtaat caacagattt tccatattct ttacattctt cgatcatttg
    18241 cttggctgtt tcagtttgct gatcgactag attttgatca ataggagcat agcccataaa
    18301 attataaaat tgctgatcga ttacgtttgc tacatatact ttcccattat tacggcgagc
    18361 cacttctaac gcttttttga aagcagcaag tgcttgttca ctaccgtcaa tacctactaa
    18421 aatgttctta tattcttgat tcatcttgac ccctcctaac agatgtcagc cttttattac
    18481 actttctagg aacgattcga tttcctgttt tgttttacga tctttattca ctagacgacc
    18541 taattctttt ccgtcttgga tcacgacaaa acttgggata ccaaaaatgt tccattcagc
    18601 ggctacatct atgtacttgt cacggtctac ttcaataaat tgccaatcag aaaagtcatt
    18661 ttcgatttca ggcatttgcg gtttgataaa acggcagtct ccgcaccagc cggcagtaaa
    18721 gaaaaataca ctttttccag tttctacgta gcctgctaat tcttctaaac tatttgggat
    18781 aatcattctt tttcctctac tttctgttta tgtatttttc aattaaatta tacacctttc
    18841 tttcgaacta acaaacattg atcctctttt tataaaaaaa atctgacaaa aggttgcttt
    18901 tgccagattt tatagtttca ctatcgcgtt atttcccgta tacgatcgtg ttcactgttg
    18961 atttatctaa acctttcaat gtttgaatca acatttctcg tgcagcttca aaatcgcgaa
    19021 tactgaacat cgtttgatgc gtatggatat aacgtcccac tacgccaatc actgtacttg
    19081 ggattccttc attttgtgta tgagcagcac ctgcatctgt accgcctttt gaaacaaagt
    19141 attgataagg gatgttgttt gtttcagcga tatctagcaa gtactcacgc aaacgaggaa
    19201 gcatgatcaa acctggatct tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg
    19261 tgccattttt cgtcactgtg tcatctgctg cagagcagtc tactgcaaag aacagatctg
    19321 gcttgaattt attgacagaa gctttagaac cacgcaagcc tacttcttct tggacattcg
    19381 cgcctgcaat caatgtatga cctaatgttt cattttgtaa cgcttctaaa gcttctaata
    19441 ccattgtaca gccatagcgg ttgtcccacg atttgctgat gatattcttc ccgttggctg
    19501 ttttgatcgt ttcagcgtaa ggaacgattg tgtctcctgg aagaacaccg taactcattg
    19561 cctcttctct tgattcaaac ccagcatcga acaaaatatc tgttacttct actgattttt
    19621 gtccgcttgt tcctcgtaat aaatgtgggg gaacggaaga agaaatacat ggataattct
    19681 taccagtaga ggttttaagt gtaaaacgtt gagcagaaac aacgtaaggg ttccatccgc
    19741 ctagaggtac gactttaaaa agcccgtttt cttggatttg tgtcagcatg aatccgactt
    19801 catccatatg tgctgcgacc attacacgag gtgcatccgc ttcttcatgg tggcgcagcc
    19861 caaaaatgcc gcccaaacca tctaactgta actcatctac cagcggttcc atatgacttt
    19921 ccatataggc gcgaacgtct tgttcaaaac cgcttgttcc ttgtaattct gttaactctt
    19981 tgattcgttg aaatgttttt tcttccatat gctattcgtc taaatttcag actaaactcc
    20041 tctcagactt atattgatct gcggataaaa tatgattttt tctgcagacc gatcgtattt
    20101 attatagctt attttttctg gtagcggtta tttatttcaa agaaaatatg agagtgtggt
    20161 aaaataaaca caaagagtag caagtgagaa gaaggaggta tatccacatg tatgaagaaa
    20221 gcgaattaag ctattttaaa ggcgggttag ccgttgggat cggtctagga ttattgagtg
    20281 gggtagcttc aacgatttgg tatcatcgca aaaaagtgtt atcagcagac caagtgcttg
    20341 aaaatatcaa aacatctttt ttaaaagaag gcgcaatcga aggtgcttgg atctcttttg
    20401 aaaaagaacc tattcggaaa ttcgccgttc ggtcgagtgc ttatcggggt gggctttccc
    20461 ggatagaaga tggcgaaact gtttattatg aatttttagc agatgctcat acaggaaccg
    20521 tattagacat ttcaagaaaa aagggcgcaa tagattaata cgtcaaaaaa atgaaataac
    20581 gaatacgcac ccactatcta aaagagaggt atcctatcct tcttttatag tgggtgattt
    20641 ttataaaaat agggctattt cctcttggaa tagctagtat ttgtagttat tatacggtat
    20701 tcttgaaata agattagttt tattatataa ggagagtgac aaatggaatc acgtgaaaaa
    20761 attgccgtgc tcattccttg ttataacgaa gaggcgacaa tttcaactgt tattgaagat
    20821 tttaaaaggg aattaccaga agcagatatt tatgtctatg ataataattc gaccgatcga
    20881 acatacgaat tggccgttca aggcggagca atcgtaaaga aagaaccacg tcaaggaaaa
    20941 gggaatgtaa tccgtcaaat gttttttgat attgatgccg attattattt gatggtagat
    21001 ggtgatgata cttatcctgc agaagctgca catggtttgc ttgagaaatt acgttctggc
    21061 gaagcagata tggtcattgg cgatcgtcta tcaaatggaa cctattttga tgaaaataag
    21121 cgtcctttcc atgattttgg gaataatctg gttaaaaata caattactta cttatataaa
    21181 acaaaaattc gcgatgtcat gaccggctat cgaggattca atcgaatttt tgtcaaaagc
    21241 ttccctatta tgagttctgg ttttcaaatc gaaacagaat tgacgattca tgctttagat
    21301 aaaaaattca agttcgttga gattccaatc gattatcgcg atcgtcctga aggtagcgaa
    21361 tcaaaattga atacattctc tgacggattc aaagtgatca tgatgatcat caaaatgtgc
    21421 aaagattata aacctttgct tttctttggg atctggacca tcttcttctt tttatttgga
    21481 ttggtcatgg ggattcctgt gattcgtgaa tatatgctga caagctttat tacacgcatc
    21541 ccttctgcga ttttaagtac tggattgatg actttagccc ttctttcatt agtaacaggg
    21601 ttgattttgg atacggtcgt cacaaatgcc aagaaagaat atgaattgaa tttataccat
    21661 gtctaccacg aatatcaaaa taaagaaatg aagtaggagg ccttttttat gcataagaaa
    21721 aaggagaatc tatttcctac tttttttgca actttatttg cgtcaggttt tgcttttttt
    21781 acttcttatg gtctaggaac tttgaatcca acagtaatca ttggtttagc catggttttg
    21841 ctacttgctt ttaagcaata tggaatagag ttgttttcaa taaaaaacaa agttgcagct
    21901 attgcccgat tgtttttttc tgcagctttt gctcttacac tttctattgg cagacagata
    21961 caatttcagt atgtctcagc aaactataca aagacaacga ttgctgatgg aagtattttt
    22021 tactttcttg tttatttctc catttttgtg tattcttttt tatttttaca atcgatttat
    22081 ttatcaatgc caaaactgga aacatttttt actgctcaaa acacacaggc tttctcttca
    22141 agaaaatatt ttttattctt tactgtcttt ttagttctgt gttggcttcc ttatctgttg
    22201 gcattgtatc ctggagtggt tttgcctgat tcgttaagtt cggtcgccca atctatgggt
    22261 gatgttccta taagcaatca tcatccagtc ttattcacct tactggtcaa gctatttgtt
    22321 catgatttac caactgatac gatcaatcac ggtgtatttt tattttcgat ttttcaaaca
    22381 ttgataacag cttcagcgat aagttattct ctttgctggc tgttaaaaag aaacatccat
    22441 ttttttccgg tactcattag tttatgctat tttgcatttg caccggtttt tccaatctac
    22501 gccttgaaca tgcaaaaaga tactttgttc tctatcgctt gctttttatt ttccttagcg
    22561 gtttttgact ttatttttca acgagaaaag ccaacgattc tcaatcagat ttacgtaatc
    22621 atccttgctt tactgaccac ctatctaaga aataatggga tttatgtagt aaccggcaca
    22681 ggtttgattt gcggcttgat tattttgaaa caaaaaaggt atcaagcatt ctttcaagtg
    22741 gtgctgggtt atacagtgat tgctggacta ctcatccacc cgttgatggc tcgttatgct
    22801 attcctacag aaactgctga acaattaggt gttcctttgc agcaaatttc tagaactatt
    22861 gttatggatg gtgacttgtc taagaaagat aaagcattca tgaatcagct actcccgata
    22921 aaagattatg aagcgtattc tcctagtctt gcagactcaa tcaagtggca taaaaatttt
    22981 aacaatgagt ttttgaatac acataaaaaa gaatttctat cactatgggc aagaaattta
    23041 ccaagtaata taaaaatcta tacagatgct tatattttag aaacgttcgg gttctgggta
    23101 cctggtacaa aaaatagtta cggcttttta gatactcgtg taaacacgaa taattatggt
    23161 atgaagcaag tagatttgat tgaacatctt actggattgt caggaatgaa aaaaatcatc
    23221 gatgctcgtg atttctttgg ttccggcaca ttactgtggt tagctatcgg cagtgcactt
    23281 ttagcaatct ttaagaaaaa atatcggtat ttactccttt ttcttcccgg tatgcttgtt
    23341 tttgctacta ttatgattgc tactcctgtg gcttttagtt tacggtatgt gtttgtttta
    23401 gcattaggtc tacctgttta tatcctctct ccttttttac tagataaaag agaaaaaatg
    23461 aaaacagata taggagatac tcatgaaact atttaaaaag atattttttg cttttgttct
    23521 cttgatcgct agctattgcg tactactgac aattactttt ttcattccgc agcactctat
    23581 tgaatcgaat gcagaaaaat ctctacagat ggttgaaaaa gaaggaatgt atccttctat
    23641 caatcaagga aatatgctag gcactcggtt agataatttt actgatcatt tgatgatacg
    23701 aaaaacaaaa gcagatcctg aattgaactc cttggagaac gcgatgtcta tggcggatta
    23761 tccccgttat tggcatggtt atcagctttt tttacgacct ttactgctag ggatgagttt
    23821 ggggagtatt cgaatgattt acgcagccat tctttttctg ctgattggat tgacgagcta
    23881 ctttttaacc aagtgtagcg acatctattt aacgacagcc ttgttgattt cgcttgctat
    23941 aggaaatgct gcaacctttt tcttcagtat gcaattttca aatatttgga ttttgacttt
    24001 attgtctgta ttactatttt taacaaaacc acaactctcc aacaccaacc aacaattatt
    24061 cttgtatttc tttgtcattg gttctttagc taactttttt gacttgttga caacaccggt
    24121 catctcttgg gggatacctg tgatcattat ttattatgta acaaataagt atttaatgga
    24181 taaacgcagt tcattgagca aacaagtagg cagttttgtt tttaccggta tcttttgggg
    24241 attaggctat ggtttgacat gggtggctaa atggatccta tcatcgatca tattgaacaa
    24301 aaacattgta aaagatgcaa tcaaccaaat cctctttcga acggaaggaa acgacaaata
    24361 cccgttgcat cgattagaga tgctgaaaag caatattaga ttgatgtatc caaaagtagc
    24421 aatattattg ctattgatta cttgtcttgt ttttctctat tttgcttatc gtaaacgaaa
    24481 tagctggcgt ccgttccaac ttctagtttt gtttgtttta gctgctgtaa cgccgtatat
    24541 ttggtacaat attttagcta atcactcaca gattcattat tggtttacgt atcgtacgca
    24601 aatcatcaca agttttgcta tacttagtgc tttcgccttt ttgatccctc ctgagaaatc
    24661 aaaagacgaa ctaaattact tttaagaata ccgatgtact aattcatttt taaacataaa
    24721 aactccagct gtcagagata gccggag
//
LOCUS       DACCCR010000034        24309 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCCR010000034 DACCCR010000000
VERSION     DACCCR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.035"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="IVK93_001444"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="IVK93_001444"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP6386739.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IVK93_001445"
     CDS             complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IVK93_001445"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP6386740.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IVK93_001446"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IVK93_001446"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP6386741.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="IVK93_001447"
     CDS             complement(3376..4683)
                     /locus_tag="IVK93_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP6386742.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="IVK93_001448"
     CDS             complement(4686..5267)
                     /locus_tag="IVK93_001448"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP6386743.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="IVK93_001449"
     CDS             complement(5839..7131)
                     /locus_tag="IVK93_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6386744.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="IVK93_001450"
     CDS             complement(7128..7751)
                     /locus_tag="IVK93_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386745.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="IVK93_001451"
     CDS             complement(7754..8590)
                     /locus_tag="IVK93_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6386746.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="IVK93_001452"
     CDS             complement(8607..9494)
                     /locus_tag="IVK93_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6386747.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="IVK93_001453"
     CDS             complement(9510..10793)
                     /locus_tag="IVK93_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6386748.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="IVK93_001454"
     CDS             complement(11476..12159)
                     /locus_tag="IVK93_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP6386749.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="IVK93_001455"
     CDS             complement(12177..12794)
                     /locus_tag="IVK93_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP6386750.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="IVK93_001456"
     CDS             complement(12804..13628)
                     /locus_tag="IVK93_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP6386751.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="IVK93_001457"
     CDS             complement(13621..14181)
                     /locus_tag="IVK93_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386752.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="IVK93_001458"
     CDS             14355..15311
                     /locus_tag="IVK93_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6386753.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="IVK93_001459"
     CDS             complement(15616..16572)
                     /locus_tag="IVK93_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP6386754.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16676..17278
                     /locus_tag="IVK93_001460"
     CDS             16676..17278
                     /locus_tag="IVK93_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6386755.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17366..18865)
                     /locus_tag="IVK93_001461"
     CDS             complement(17366..18865)
                     /locus_tag="IVK93_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP6386756.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18881..19405)
                     /locus_tag="IVK93_001462"
     CDS             complement(18881..19405)
                     /locus_tag="IVK93_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386757.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19703..20452
                     /locus_tag="IVK93_001463"
     CDS             19703..20452
                     /locus_tag="IVK93_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP6386758.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20443..21228
                     /locus_tag="IVK93_001464"
     CDS             20443..21228
                     /locus_tag="IVK93_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6386759.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21197..21913
                     /locus_tag="IVK93_001465"
     CDS             21197..21913
                     /locus_tag="IVK93_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6386760.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22015..23034)
                     /locus_tag="IVK93_001466"
     CDS             complement(22015..23034)
                     /locus_tag="IVK93_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP6386761.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23053..24111)
                     /locus_tag="IVK93_001467"
     CDS             complement(23053..24111)
                     /locus_tag="IVK93_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP6386762.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7214 a         5229 c         4229 g         7637 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagtttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
    15421 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    16621 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    16681 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    16741 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    16801 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    16861 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    16921 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    16981 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    17041 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    17101 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    17161 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    17221 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    17281 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    17341 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    17401 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    17461 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    17521 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    17581 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    17641 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    17701 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    17761 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    17821 gttctgcttc agcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    17881 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    17941 tgatttcttt ttcttccagt tcgatttgtt tttggcgttc tacgatttta atctgcattt
    18001 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    18061 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    18121 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    18181 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    18241 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    18301 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    18361 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    18421 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    18481 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    18541 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg
    18601 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    18661 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    18721 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    18781 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    18841 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    18901 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    18961 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    19021 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    19081 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    19141 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    19201 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    19261 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    19321 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    19381 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    19441 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    19501 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata
    19561 aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa
    19621 cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt
    19681 gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat
    19741 tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac
    19801 acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt
    19861 tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc
    19921 tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa
    19981 agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat
    20041 tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg
    20101 acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt
    20161 ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga
    20221 acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc
    20281 ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga
    20341 gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt
    20401 aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata
    20461 gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca
    20521 actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa
    20581 tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct
    20641 ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa
    20701 aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt
    20761 tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc
    20821 gattctttta tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta
    20881 gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag
    20941 gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat
    21001 gacggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg
    21061 aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt
    21121 ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca
    21181 aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc
    21241 agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag
    21301 aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga
    21361 tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa
    21421 tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat
    21481 ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc
    21541 gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa
    21601 attgatcgat caagtattca accacaccac tcccattcac gaaataccat cattcatcga
    21661 tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga
    21721 agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt
    21781 caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt
    21841 ggaaatcggc cggccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac
    21901 agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac
    21961 aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct
    22021 taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc
    22081 acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc
    22141 tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc
    22201 tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac
    22261 atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc
    22321 ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa
    22381 tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat
    22441 atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc
    22501 tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc
    22561 cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact
    22621 aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt
    22681 atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata
    22741 cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt
    22801 gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt
    22861 ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac
    22921 gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa
    22981 tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc
    23041 ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca
    23101 tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag
    23161 tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat
    23221 catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa
    23281 aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata
    23341 ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat
    23401 tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca
    23461 cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt
    23521 aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc
    23581 gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact
    23641 catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga
    23701 atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt
    23761 agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa
    23821 aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt
    23881 gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc
    23941 tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat
    24001 cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact
    24061 gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct
    24121 cctaattatg ttcatttatt cacaataaaa aaagctttta ttttgctaat acgtattaac
    24181 atttttaatt ttatctttac attgttactt attgaacaat aggaaattaa agaaactttt
    24241 gaaacttatg taaagtgatt tttactagaa cacaaaaaaa accttaaaac caaacttaag
    24301 aagtttagt
//
LOCUS       DACCCR010000035        23959 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCCR010000035 DACCCR010000000
VERSION     DACCCR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.036"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            476..1339
                     /locus_tag="IVK93_001468"
     CDS             476..1339
                     /locus_tag="IVK93_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6386763.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="IVK93_001469"
     CDS             complement(1468..3090)
                     /locus_tag="IVK93_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6386764.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="IVK93_001470"
     CDS             3339..3860
                     /locus_tag="IVK93_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386765.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="IVK93_001471"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="IVK93_001471"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP6386766.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="IVK93_001472"
     CDS             5941..6759
                     /locus_tag="IVK93_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP6386767.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="IVK93_001473"
     CDS             complement(6862..7059)
                     /locus_tag="IVK93_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386768.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="IVK93_001474"
     CDS             7228..7806
                     /locus_tag="IVK93_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6386769.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8851..9354)
                     /locus_tag="IVK93_001475"
     CDS             complement(8851..9354)
                     /locus_tag="IVK93_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6386770.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="IVK93_001476"
     CDS             9713..10738
                     /locus_tag="IVK93_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6386771.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11085..11963
                     /locus_tag="IVK93_001477"
     CDS             11085..11963
                     /locus_tag="IVK93_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386772.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12439..12555
                     /locus_tag="IVK93_001478"
     CDS             12439..12555
                     /locus_tag="IVK93_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP6386773.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13483..13821
                     /locus_tag="IVK93_001479"
     CDS             13483..13821
                     /locus_tag="IVK93_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386774.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13915..14145
                     /locus_tag="IVK93_001480"
     CDS             13915..14145
                     /locus_tag="IVK93_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386775.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14545..14916
                     /locus_tag="IVK93_001481"
     CDS             14545..14916
                     /locus_tag="IVK93_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP6386776.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15190..15882)
                     /locus_tag="IVK93_001482"
     CDS             complement(15190..15882)
                     /locus_tag="IVK93_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6386777.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15885..16670)
                     /locus_tag="IVK93_001483"
     CDS             complement(15885..16670)
                     /locus_tag="IVK93_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6386778.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16907..18307
                     /gene="lacG"
                     /locus_tag="IVK93_001484"
     CDS             16907..18307
                     /gene="lacG"
                     /locus_tag="IVK93_001484"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP6386779.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18332..18649
                     /locus_tag="IVK93_001485"
     CDS             18332..18649
                     /locus_tag="IVK93_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6386780.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18662..20335
                     /locus_tag="IVK93_001486"
     CDS             18662..20335
                     /locus_tag="IVK93_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6386781.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20349..21980
                     /locus_tag="IVK93_001487"
     CDS             20349..21980
                     /locus_tag="IVK93_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP6386782.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22852..>23959
                     /locus_tag="IVK93_001488"
     CDS             22852..>23959
                     /locus_tag="IVK93_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP6386783.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQ"
BASE COUNT         7476 a         4070 c         4835 g         7578 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt atatttattc tatcaaaaaa
    10921 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
    10981 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
    11041 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
    11101 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
    11161 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
    11221 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
    11281 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
    11341 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
    11401 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
    11461 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
    11521 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
    11581 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
    11641 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
    11701 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
    11761 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
    11821 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
    11881 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
    11941 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
    12001 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    12061 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12121 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
    12181 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
    12241 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
    12301 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
    12361 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
    12421 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
    12481 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
    12541 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
    12601 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
    12661 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
    12721 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
    12781 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
    12841 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
    12901 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
    12961 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
    13021 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
    13081 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
    13141 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
    13201 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
    13261 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
    13321 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
    13381 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
    13441 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
    13501 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
    13561 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
    13621 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
    13681 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
    13741 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
    13801 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
    13861 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
    13921 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
    13981 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
    14041 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
    14101 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
    14161 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
    14221 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
    14281 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
    14341 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
    14401 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
    14461 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
    14521 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
    14581 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
    14641 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
    14701 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
    14761 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
    14821 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
    14881 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
    14941 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
    15001 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
    15061 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
    15121 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
    15181 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
    15241 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
    15301 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
    15361 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
    15421 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
    15481 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
    15541 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
    15601 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
    15661 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
    15721 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
    15781 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
    15841 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
    15901 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
    15961 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
    16021 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
    16081 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
    16141 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
    16201 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
    16261 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
    16321 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
    16381 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
    16441 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
    16501 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
    16561 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
    16621 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
    16681 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
    16741 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
    16801 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
    16861 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
    16921 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
    16981 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
    17041 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
    17101 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
    17161 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
    17221 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
    17281 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
    17341 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
    17401 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
    17461 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
    17521 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
    17581 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
    17641 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
    17701 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
    17761 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
    17821 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
    17881 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
    17941 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
    18001 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
    18061 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
    18121 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
    18181 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
    18241 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
    18301 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
    18361 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
    18421 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
    18481 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
    18541 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
    18601 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
    18661 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
    18721 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
    18781 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
    18841 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
    18901 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
    18961 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
    19021 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
    19081 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
    19141 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
    19201 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
    19261 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
    19321 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
    19381 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
    19441 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
    19501 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
    19561 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
    19621 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
    19681 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
    19741 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
    19801 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
    19861 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
    19921 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
    19981 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
    20041 actttgtgct ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc
    20101 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
    20161 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
    20221 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
    20281 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
    20341 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
    20401 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
    20461 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
    20521 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
    20581 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
    20641 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
    20701 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
    20761 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
    20821 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
    20881 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
    20941 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
    21001 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
    21061 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
    21121 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
    21181 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
    21241 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
    21301 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
    21361 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
    21421 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
    21481 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
    21541 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
    21601 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
    21661 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
    21721 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
    21781 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
    21841 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
    21901 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
    21961 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
    22021 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
    22081 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
    22141 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
    22201 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
    22261 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
    22321 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
    22381 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
    22441 gggattcccc ctccatatta tgttggctct ttattcccac tctgttttca acgtaaagtg
    22501 tataaaacag aatgcgatac tatcaaggga gttaaaacaa tatacagaac agtaaaacac
    22561 tataaataaa aataaaagtg caataaaagt gcaatttttg cacctaatca tactctgttt
    22621 atatatgaaa actagattct gaatccaata tttggttaac gagaggcgtt cgaatttgaa
    22681 cgtcttttat atattacttt caaataaata tgaggtagaa tactatctat tttcttttta
    22741 acccaattgt tataatggag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc
    22801 ttctgtagaa tagagttacc acaacacatt cacagaagag aggacttccc tatgaacgat
    22861 tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga attattccgt
    22921 gtccatttgg agaaagcagt caatacgctt ctcaaaacgg agttaacggc tttcctagat
    22981 tacgaaaagt atgatcgcat tggttttaac acgggtaatt ctcgtaacgg ctcctatgac
    23041 cgtacggtca agaccgagta tggggaactt catctccaga ttccgcgcga ccgcaatggt
    23101 gagttcaagc aacagactgt tcctgcttat agacggacga atgacacgtt agaggagacc
    23161 gtcattcacc tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa
    23221 atgtatgggc attactacac gccccaaacc atgtccaata taacaaaatc atttacagaa
    23281 gaggtaacgg cgtttaatgg gcgggagctt catgaccgtt atgctgctat ttatatggac
    23341 gcaacgtata ttccgttaaa gcggaaaacc gtcgccaagg aagctattca tatcgcagtt
    23401 ggcattcgcc cggacggatc aaaggaagta ttgagctatg cgattgcacc gactgaatcc
    23461 atcacgattt gggaggaaat tttattggac cttcaagagc gcggtttgaa aaatgtcctc
    23521 ctgttcatca cggatggctt aaaggggatg gtaggagcga ttagtcggtt ctatcccaaa
    23581 gctcgttttc aacattgttg tgtacacgtt tcccgtaata tcagtcataa agtgcgtgtc
    23641 gatgatcgta aggaagtctg tgatgatttt aaaatggtgt atcaagcctc atctaaagag
    23701 gtggcgttgg aagcacgtgg tacttttgcg gaaaaatgga aaaccagcta tccaaaagtg
    23761 gttgaatcga ttctttcgaa cgatcacttg ctcactttct atgatttccc cttggccata
    23821 cgcaagagta tttattctac gaacttgatt gaatccttta ataaacaaat caagaaatac
    23881 agccaccgca aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct
    23941 tttgatactt acaaccaaa
//
LOCUS       DACCCR010000036        23926 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCCR010000036 DACCCR010000000
VERSION     DACCCR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.037"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            260..745
                     /locus_tag="IVK93_001489"
     CDS             260..745
                     /locus_tag="IVK93_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP6386784.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            738..1421
                     /locus_tag="IVK93_001490"
     CDS             738..1421
                     /locus_tag="IVK93_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP6386785.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1547..4192
                     /gene="polA"
                     /locus_tag="IVK93_001491"
     CDS             1547..4192
                     /gene="polA"
                     /locus_tag="IVK93_001491"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP6386786.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4237..5073
                     /gene="mutM"
                     /locus_tag="IVK93_001492"
     CDS             4237..5073
                     /gene="mutM"
                     /locus_tag="IVK93_001492"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP6386787.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            5037..5666
                     /locus_tag="IVK93_001493"
     CDS             5037..5666
                     /locus_tag="IVK93_001493"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP6386788.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5718..6050
                     /locus_tag="IVK93_001494"
     CDS             5718..6050
                     /locus_tag="IVK93_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6386789.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6362..6853
                     /gene="nrdR"
                     /locus_tag="IVK93_001495"
     CDS             6362..6853
                     /gene="nrdR"
                     /locus_tag="IVK93_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP6386790.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6876..8249
                     /locus_tag="IVK93_001496"
     CDS             6876..8249
                     /locus_tag="IVK93_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP6386791.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8249..9175
                     /gene="dnaI"
                     /locus_tag="IVK93_001497"
     CDS             8249..9175
                     /gene="dnaI"
                     /locus_tag="IVK93_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP6386792.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9304..9744
                     /locus_tag="IVK93_001498"
     CDS             9304..9744
                     /locus_tag="IVK93_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP6386793.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            9888..10589
                     /locus_tag="IVK93_001499"
     CDS             9888..10589
                     /locus_tag="IVK93_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP6386794.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            10586..11707
                     /locus_tag="IVK93_001500"
     CDS             10586..11707
                     /locus_tag="IVK93_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP6386795.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            11722..12954
                     /gene="pepT"
                     /locus_tag="IVK93_001501"
     CDS             11722..12954
                     /gene="pepT"
                     /locus_tag="IVK93_001501"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP6386796.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            13086..13994
                     /locus_tag="IVK93_001502"
     CDS             13086..13994
                     /locus_tag="IVK93_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP6386797.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            14029..14301
                     /locus_tag="IVK93_001503"
     CDS             14029..14301
                     /locus_tag="IVK93_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP6386798.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            14312..15358
                     /locus_tag="IVK93_001504"
     CDS             14312..15358
                     /locus_tag="IVK93_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP6386799.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            15609..18218
                     /gene="clpB"
                     /locus_tag="IVK93_001505"
     CDS             15609..18218
                     /gene="clpB"
                     /locus_tag="IVK93_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP6386800.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(18369..19040)
                     /locus_tag="IVK93_001506"
     CDS             complement(18369..19040)
                     /locus_tag="IVK93_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386801.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(19033..19527)
                     /locus_tag="IVK93_001507"
     CDS             complement(19033..19527)
                     /locus_tag="IVK93_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6386802.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(19547..20767)
                     /locus_tag="IVK93_001508"
     CDS             complement(19547..20767)
                     /locus_tag="IVK93_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6386803.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            20914..22749
                     /gene="lepA"
                     /locus_tag="IVK93_001509"
     CDS             20914..22749
                     /gene="lepA"
                     /locus_tag="IVK93_001509"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP6386804.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(22843..23925)
                     /locus_tag="IVK93_001510"
     CDS             complement(22843..23925)
                     /locus_tag="IVK93_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6386805.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         7943 a         4312 c         5079 g         6592 t
ORIGIN      
        1 aaaaatcttg ccaagccccc ttttttgcaa cttatgataa acggtgttca aaagcttgca
       61 tctgcggtaa taagcccaat caaatcaaaa acatgagaag ctgttttttc ttggctgttc
      121 ttattatttg atgcaggaca gctttttcac gacctctgta aactatagga atctgttaaa
      181 tcaagggagc agaacttgcc ttttacgcta cttttggtac aataatgtat gattaaaaat
      241 aaactggagg gtttctatct tgaacatagg taagccgaga aaactaggaa aaacaataaa
      301 tgaaatcgag gaaggcgact cgttatcctt aacagaatct attgaagata accagctttt
      361 gctgtatttg ggattgacga atgatgcaaa tcctttgtat atccaacatg actttgcaca
      421 aaaaacagaa tatcaacagc caatcgttcc atcagtgatg ctgatgggga tcatcacaag
      481 tgcaatttca aagcatcttc ctggaccagg atcacatgtt gtcaattttt ctgtcaattt
      541 tattgaaccg gttttccatt atgaaacact gacttttcag tttgaggtca taaaagtaga
      601 taagatgaag gatgtggtga cgatttctgt agaagcggag aatacgaacg aagatcgtgt
      661 gttggatgct gtcgttatgg tccaaccgcc acaagttcct gaattggaaa tagcaatcga
      721 aagtgaaggt gaattgaatg aataatccaa gtacatcaat gtcaggtatc aatcgttttt
      781 atgcaaaagt ttatggattt ttaggtatgg gacttgccct tagtgcagtc acttcttact
      841 tggctctttg ggtatttcct ttgcaattag caacttttat caataatttt ccactaggct
      901 ttgtgggtct gtggatcgtt gaactgattt tagtaatggt tctaggcgta aaagcacaaa
      961 agaatccgag cttagcaatc ggtggattta ttgcctactc tcttttgaat ggcataacat
     1021 tagcggttac tcttgcatac tacgatattg gaacagtaac gcaagctttc ttgacagcta
     1081 ctttgatgtt catggggatg tctgttgtcg gagtaacaac aaaaagagat cttagcgggg
     1141 ttggaagagc aggacaggca gctttaatcg gagtgatcat tgcactagtc ttgaacatct
     1201 tcattttgag aagtagcgta gttgagctgt tcatctccct actgttagtc gtcatctttg
     1261 cagggattac agcttatgat aaccaaagca tccgtaatat ctatgtccag acaaacggaa
     1321 cagcacatag tggaattgct gtattcatgg cattgaagct gtatttggac tttatcaact
     1381 tgttcttggc gtttctgaga atctttggac ggaacaacta aaaaagaaaa aaattcatca
     1441 gttggggcta taccgaatta tttggtatgg tcccgctttt tcttttcgca ttccgcagga
     1501 ttctttgtta gaatgaataa gaactttaca gtaaggagtt aagaagatga caaaaaataa
     1561 attattactc gttgatggga acagtgtggc ttttcgcgca ttctttgcgc ttcacaattc
     1621 tcttgaacgt ttcaaaaata gaaacggtct tcatacaaat gcaatctatg cattcaatac
     1681 gatgtttgaa aatgttatgc aaaaagagca accgactcat gttctggttg cgttcgatgc
     1741 aggaaaaacg acttttcgga cagaaatgta tgcggagtac aaaggtggaa gatccaaaac
     1801 acctggcgaa ttcaaagaac aaatgccata tatacgtgat ttgttgacag gactaggggt
     1861 acagtattac gaattgccca attatgaagc agatgatatt atcggtactt tggcagaaaa
     1921 agtagacaaa gatcaatttg atgtcgtcgt gctttcaggt gatcgggatt tgactcaatt
     1981 agctagtaaa gaagtaaaag tggatatcac tgtaaaaggt gtcagcgata tcgaaagcta
     2041 tacaccagaa catgtagcgg aaaaatatga tgggttgacg ccaaaacaga ttattgacat
     2101 gaagggacta gccggagatg cctctgacaa tatccccggt gtgactaaaa tcggtgaaaa
     2161 aacagctatc aagctattga aacaatatgg ctcagtcgaa ggaatttatg aacatatcga
     2221 tgaaatgaag caaagcaaaa tgaaagaaaa tctgataaat gacaaagagc aggctttctt
     2281 atcgaaaaaa ttggcgacca tcaatacttc agcgcctgtg gatgtctcta tcgactcatt
     2341 gaagtatgaa gggaaaaatc tcgataaact cgttccgttt tataaagaaa tggatttcaa
     2401 tcagttcctt tctaaattga atatcgtaga agaagaagta aaaatggatg atattttgtt
     2461 tgaagtcgtt catgaattca aagaggaaat gtttactacg gatatggcgt tgtacgtgga
     2521 aatgatgggc gacaattatc atacagagga aatcgtcgga gtagcgtggg ggacagaaaa
     2581 gaaaatttat gtcacgaacg atctatctgt attcgaaaac cgagcgttcc attcttggat
     2641 cactgatccg actcggctga aaaaagtata tgatgcgaaa cgaacttatg ttgctttaaa
     2701 ccgctatact ggaaaaacga aaggaatcga ttttgatgtc ttgctaggtg cataccttct
     2761 cgatacgaat gaaaaaagca cagatatcga aggaatagct gctcattacg gatacaacga
     2821 tatccaatca gatgaagctg tatacggcaa aggggcgaaa aaaggtttac cagaagaaga
     2881 ggaactcttt tttgctcatt tagcgcgaaa agtagctgca atcaatgcgt tgacaccaaa
     2941 attgagccaa gaactggcag ataaaaacca agaagatctt ttccggaaaa tggaactgcc
     3001 attgtctatc attttggcag agatggaaat aagcgggatc aaagtagatg cgactcgctt
     3061 acaagaaatg aagggcgagt tctctgctcg cttaagagag atcgaacaaa aaatctacga
     3121 agaagccggc gaagaattca atttgaattc accaaagcaa ttaggagtga tcttatttga
     3181 aaaaatggga ttacccgtta tcaaaaaaac aaaaactgga tattccacag ccgtagatgt
     3241 actggaacag cttcgtgaac aagcaccaat cgtagaggat atcctcacct atcgtcaaat
     3301 tgcaaaaatt caatctacct atgtcgaggg actgctgaaa gtgatccaat cagatggaaa
     3361 agtgcatacc cgttatgtcc agacattgac ccaaacagga cgattaagct ctgttgatcc
     3421 taatttgcag aacatcccga ttcgattaga tgaagggaga aagatacgtc aggcatttgt
     3481 tcctagagaa aaagactggc tgatttattc ttctgactat tcacaaatcg agttacgcgt
     3541 acttgcgcat atctcaaatg atgaacattt gaaagctgcc ttcctagaag gtcaagatat
     3601 ccatgccagc acagcgatgc gtgtatttgg aatcgaaaag gcagaggatg tcacaccaaa
     3661 tatgcgtagg caggcaaaag cagtgaattt tggtatcgtg tatggcatct ctgattacgg
     3721 actttcacaa aatctaggaa tcagccgaaa agcagcacag caatacatcg atacgtactt
     3781 tgaaaaatac ccaggtgtca aagaatatat ggaaagaatc gtacgcgaag caaaagacca
     3841 aggctacgtc gaaacgctgt atcaccgccg tcgatatctg ccagacatca attcaagaaa
     3901 ttacaatatc cgttcttttg cggaacgaac agctatcaat acaccaatcc aaggaagtgc
     3961 tgctgatatt ttgaaaatcg cgatgatcga attggataaa cgattaaaag aaacaggtct
     4021 tcaagctacg atgctgttac aagttcatga tgaacttgtc ttcgaggtac cggaaaaaga
     4081 acttgaatca ttagataaat tggtgaagga agttatggaa caagctgttt ctcttcatgt
     4141 accactgatc actgacagca gttggggaaa aacgtggtac gaagcaaaat aaacattgtt
     4201 taagacagag caatataatg aaggtgaaaa gaaaaaatgc cagaattacc agaagtagaa
     4261 accgttcgta aaggattgga acggttagta gtaggaaaaa cgattcaaaa agttcaggtc
     4321 ttgtggccca agatcatcga gcaaccagag acacccatct ttgaggcttc tcttgttggc
     4381 gaaaccatcc aatccattgg acgtagaggg aagtttttaa tttttcatct cgatcattgt
     4441 gaactgatct cacacttgcg aatggaagga aaatatcaat ttacgaaaga aaatacacca
     4501 atcgataaac atacgcatgt acttttcttt tttgaggatg gaagccaact tcgctacaac
     4561 gatgtacgaa aatttggtcg tatgacaatc gtcgaaaaag gagcaagtgc aacttaccga
     4621 ggaatcatga aattgggacc tgagcctttg ccagatagct tcttattagc agatttcgca
     4681 aatggactga aaaagtccca caaagcaatc aagccattgc tgctagacca acgtctagtg
     4741 actggtctag gtaatattta tgtagatgaa gcattatggg aagcgaagat ccatccagaa
     4801 caaccggcga acacactgcg ccccaaagag gtagaacaat tacgtctatc gatcattgat
     4861 gttttggatc gtgcaataga agccggaggt accacgattc gcagttattt gaatgcatta
     4921 ggagaatcag gcggatttca agtagcactg catgtctatc agcaaactgg caaaccatgt
     4981 atacgttgtg gaacgccgat cgtgaaaaca aaagtggcac aaagggggac gcactattgt
     5041 ccgaattgtc aaaggctaaa aatcgcaaag taggatttgt tttaggtttg acgggtggaa
     5101 tagctaccgg taaaagtacg gcagccaaag tctttcagtc tcatggattt cctttgatcg
     5161 atggcgatgt gattgccaga gaaactgttg agcctggaac gcttgctctg aggaaaattg
     5221 tatcagtgtt tggccaagag gtattgcagc ctgatgggca actagatcga gggaaacttg
     5281 ggatgattgt ttttccatca aaggaaaaac ggcaaaaact tgatcagcta ttagatccgt
     5341 ttatccgaaa agcgataaaa gatcaaatcg cctctctaag ttccaatcat cctttagtaa
     5401 ttgtagatat cccattgctc tacgaaggtc attatgacca ctatatggat gcagtagctg
     5461 tcgtttatac tacgccagaa actcagcttc agcgtttgat gaaacgtaat caactgacgc
     5521 tagaacaagc gcagcagaga attagtagtc aacttccaat cgaagaaaaa aagcagcgtg
     5581 cggatatttt gttcgacaac aatggaacaa aagaaaaact tgtgggacaa atcgaaaatt
     5641 ggctttttgc taatcgcttt ctttgagtat ctgtgtcaat tttgctatcc tgagtatata
     5701 gaaagtgagg gatagcgatg ggatcattaa aaaatcaaaa ggataaagtc gtaggtaacg
     5761 caaaagaaaa aatcggcgag atgctcaata aagaaaatct aatcgatgaa ggtcgtacgc
     5821 aatcagaaac agcagaagaa aaagaaaagc agtaccaagc aagaatcgat gaaatagaag
     5881 accagcaggc agaagaagaa gcgaagcggc aagatttgac tcaagaacga aaaaatccga
     5941 cacctgatcg tagtaaaaca ccgctcccag aaagtgacca catttccgat gagcaaaaaa
     6001 tcgaggaaca gcgaatgaaa cactatacag gaatcgaaga gaagttataa aatagtaaaa
     6061 aatcgagatg tgacttgaag cagatgagaa ggtgttcatc tgcttcaagt tttttgtgct
     6121 agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga tcagtattgg
     6181 aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga aaaaattgtt
     6241 cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg tgttataata
     6301 caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga ggtgagacaa
     6361 aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc gtcaagcaga
     6421 tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc gattcactac
     6481 atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg aacgcgaaga
     6541 gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa gacctgtagc
     6601 tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca gtctcggaga
     6661 aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag ttgatttaga
     6721 cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata tgtctgtttt
     6781 cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag agaataataa
     6841 agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg aaagagcttc
     6901 aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac agcaagaacg
     6961 ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc tatttcaccc
     7021 tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat gcagatctgc
     7081 ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag ctagaaggaa
     7141 tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt tttatttatg
     7201 agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg gcttttcttc
     7261 ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt gaacctaaaa
     7321 ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt tatcgcttca
     7381 atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa tcttttgaag
     7441 aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag tttttgaatg
     7501 attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa caagtcgaca
     7561 tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc agtcaatctt
     7621 atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt ttggcaatgc
     7681 agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac agtaatgaac
     7741 cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa gcacagagca
     7801 ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat gtttctaaac
     7861 aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt agcgtgatca
     7921 atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat gccagttatg
     7981 taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa gaagcgatcc
     8041 agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag caaacaaatt
     8101 atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc aaccagccaa
     8161 aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt tttaaagaat
     8221 acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat tgaagaggat
     8281 catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag tcttaaatga
     8341 tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag cggatattga
     8401 aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc aaaaaaacga
     8461 tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc attatgtgga
     8521 tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag aaatcagaag
     8581 ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg aaaactttga
     8641 acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag agaaatatac
     8701 tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg gtattggaaa
     8761 aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca ccacgacgct
     8821 agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag atcaagtggc
     8881 agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca ttggtgcaga
     8941 tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat atcggatgca
     9001 ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg aacagcattt
     9061 gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa tggaacgcat
     9121 acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg gttgaacagt
     9181 tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat cattatatgg
     9241 acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa cggaggattt
     9301 tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct tttagcaggc
     9361 tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg tcaaacagta
     9421 aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat tgaaagtatt
     9481 actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt catggccaaa
     9541 gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt aaatgctact
     9601 gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt ccaatttaca
     9661 ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg agattcgatt
     9721 attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga aaatgttgtt
     9781 ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat tcctagtctc
     9841 cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg aaccatttag
     9901 atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct cgtttagcag
     9961 acattggatc agatcacgct tacttacccg ttgcattgat attaagagga aaaatcgaat
    10021 atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag caagtcaaaa
    10081 aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat gctatccagc
    10141 cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg atccgagata
    10201 tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg atccttcagc
    10261 caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat aaaatccaat
    10321 cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc gaaaaagaac
    10381 aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg cttgaagaga
    10441 aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa gctatccgtg
    10501 aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag caggaaatcc
    10561 aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca tccaaaagtt
    10621 tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac tgcatatcgg
    10681 aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc cagaagtggt
    10741 cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc caatctttcg
    10801 tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag atttgttgaa
    10861 acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg atggactgaa
    10921 tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa agactcatga
    10981 gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga aaatgcgtga
    11041 agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa gttttacctc
    11101 gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg ggaaagtagg
    11161 gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa ctatcgaaaa
    11221 acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt atgatctttt
    11281 cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat tgccgcaaga
    11341 aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag atggactgag
    11401 aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt gcggtggaag
    11461 cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata tcacgggaga
    11521 tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa ttgatcccgg
    11581 tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat cttggaaaca
    11641 agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc catttcaatt
    11701 taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt acgatatgtc
    11761 aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca gacacaagtt
    11821 gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga tgttttctat
    11881 aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca tgaagtgcgt
    11941 tccattggat ttatttccca catggataca gcagatttta atgcagtagg agtcaatcca
    12001 caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga agaacgctat
    12061 acattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac attgatcaca
    12121 acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga aatcatgact
    12181 gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa agtggctttt
    12241 ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca attcgatgta
    12301 gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga aacgtttagt
    12361 gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac ggcaaaagat
    12421 acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc agccgatgaa
    12481 gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt ggatgggaat
    12541 cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa attcgaagca
    12601 cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga tcaagaacga
    12661 atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga aaaagatatg
    12721 agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc agtgatcgaa
    12781 ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc gacgccaaat
    12841 ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt acaagtaatg
    12901 gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag ttaaaatcga
    12961 aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct gtctaaggct
    13021 aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta aataggaggt
    13081 aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat gagggtgaga
    13141 aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta tatgtagaac
    13201 tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca gaagccatag
    13261 aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc gtccaagcgt
    13321 tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact gtcaacaacc
    13381 ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg atcctggaag
    13441 gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc tctacactga
    13501 caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa cagcgttacg
    13561 gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa aaatatcatt
    13621 tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg acagtcacga
    13681 tgtctaactc cgtgggcggt tttacaaatt ttgacgactc agcgacccta gatgatggaa
    13741 aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt tatttgccta
    13801 gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca gccaaacgca
    13861 tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac acgacagatg
    13921 atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca ccaaagaaag
    13981 aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat ggatgtattg
    14041 ttacacaatc ttttgtggtt ttaccttttc attgtgaatt tttacttatt ttgtctgatg
    14101 gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa taatttgata
    14161 tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt tttccatcat
    14221 aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt tgatttgatt
    14281 ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga aaactaaaat
    14341 cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt taatcaatca
    14401 atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt tggcagcggc
    14461 tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag aaattgctga
    14521 acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct atattgcgtt
    14581 ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt tttataacaa
    14641 aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga ttatgtacaa
    14701 actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc ttttttattc
    14761 taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga tcgtgattgt
    14821 tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg tcagcttatc
    14881 gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca atcagcagat
    14941 ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc tattgcggat
    15001 ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt tgattctgat
    15061 tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct ttgcagtagt
    15121 gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg tctatttgtt
    15181 attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac tgtatcgttt
    15241 cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg aacggcgaag
    15301 agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc aagggtaggt
    15361 cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat aagataattt
    15421 tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt taagatttat
    15481 ataagtcgag aaaaaaagtc aaaaaaccat gtgtatattt tgaccaaaat tgaccaaagc
    15541 gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa ttaggagggt
    15601 atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt gctgaagctc
    15661 aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta tggaaaatct
    15721 ttttacaacc taatcatttt ggtcgaaatt tctatacgga tgccggattg gatgtcgaat
    15781 cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct ggtggaaacg
    15841 tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa gcagaccaaa
    15901 tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta gcgttgatga
    15961 aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct gaaaaagaat
    16021 tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa aaccaagaag
    16081 aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc aaaagcggaa
    16141 aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt attctttcac
    16201 gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa acggcaatcg
    16261 ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta aaagataaaa
    16321 cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt ggtgaatttg
    16381 aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt atcctcttgt
    16441 tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt atggatgccg
    16501 gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt gccacaactt
    16561 tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc tttcaaaagg
    16621 tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga ttaaaagaac
    16681 gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca gcagctactt
    16741 tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat ttgatcgatg
    16801 aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta gatcaagtta
    16861 ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa tcagatgatg
    16921 caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt gaagaagcaa
    16981 atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt tcagcaaaac
    17041 gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac tatgatttag
    17101 aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa ttgaaggaat
    17161 tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt accgaaaatg
    17221 agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt gtagaaggtg
    17281 aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt ggtcaagatg
    17341 aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg caagacccaa
    17401 atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa accgaattag
    17461 cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg atcgatatga
    17521 gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca ggctatgtag
    17581 gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat acaatcgtct
    17641 tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta caagtcctag
    17701 atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat acggttctga
    17761 tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct gatggaacga
    17821 ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc aaaccagaat
    17881 tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac aacgtgaaag
    17941 gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa gaaatcctgc
    18001 tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa ccagcatatg
    18061 gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta gctaaagaaa
    18121 tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt gatggacaac
    18181 ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag actataaaag
    18241 aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa cagtgttcca
    18301 gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt ctgtcacaac
    18361 ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta acgctcatct
    18421 tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt atttgtcagc
    18481 aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc atcagaagca
    18541 gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca gaactcccaa
    18601 aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca aggactggtt
    18661 tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag agctgtcagg
    18721 accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact gatcaaatgc
    18781 atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa aacgattgct
    18841 gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat caatgcaatc
    18901 cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt ttgtgtgata
    18961 aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc tatttgcacc
    19021 tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta cttctgaaag
    19081 caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta ttgtatataa
    19141 gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt ttttcgggtg
    19201 ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc ctttcgcata
    19261 agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta gtaaagcaat
    19321 cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc gactgtctgc
    19381 ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg aaagctcaga
    19441 agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac ttttagcaaa
    19501 cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa aaataatcgc
    19561 taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt tcttcatctg
    19621 tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc atcaagcaag
    19681 tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag acacggactt
    19741 gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg acaggatctt
    19801 caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga aggaattctt
    19861 ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc tgactatctt
    19921 caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag tagatcatgg
    19981 aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca agatagaaca
    20041 tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct cttggttcat
    20101 ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa cgccaatact
    20161 cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca aatttgtcat
    20221 aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca cttactggga
    20281 tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac atgatttccc
    20341 atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc cattgcttat
    20401 tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca tggttagcat
    20461 attctggata agttccaact ccagtcacac cacccatttt ataacgtgca ccgtgaatat
    20521 tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg aaccatccga
    20581 acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt ttattctcaa
    20641 acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc aattcattga
    20701 attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta aaatcttttt
    20761 gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat tctacaaaaa
    20821 aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt tggtataatg
    20881 gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat gaaagaacgc
    20941 caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa atctacattg
    21001 gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca agatcagctg
    21061 ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa tgctgtagaa
    21121 ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga caccccaggg
    21181 cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg tgccgtttta
    21241 gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta tttagcgtta
    21301 gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc agcagatccc
    21361 gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga agctgtatta
    21421 gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt ggaatatgtt
    21481 cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga ctctatttat
    21541 gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt caaacccgga
    21601 gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt cggggtcttc
    21661 tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta tatcacagca
    21721 agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc agacaatcct
    21781 gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg tggtctttat
    21841 ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact gcaattgaat
    21901 gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg gttccgctgt
    21961 ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg ggaattcaat
    22021 ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac ggatggtacg
    22081 actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca ggatgtagaa
    22141 gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc agtaatggaa
    22201 ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga ttaccgagta
    22261 aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga caaattgaaa
    22321 tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca aaaaagcaag
    22381 ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag ctttatcgtt
    22441 caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa aaaactaatc
    22501 ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat cgttgcccgt
    22561 tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg agatgtttct
    22621 cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa acaaatcggc
    22681 tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggacga ggacgaaccg
    22741 aagaaatagc aagggtttgt ttgaaaacag tgtaaaaaat aaaccatgtt tacatggatt
    22801 tgccaccggt ttgccaccgg tggttttttt cggtggcaaa aatcaagaat tctttctaag
    22861 attttccatg atatcgaccg tttcatttct catcttatct gttacatgcg agtaagtatc
    22921 catcgtaatt gaaattctgc tgtggcccaa tcgttcagag atttctttca tttttgcgcc
    22981 attttcgaga agaagtgtag catgagtatg tctgagagaa tggaagttaa aagagaggga
    23041 gagtgcattc gatatcctcc tagtattcca tttcactaca cttggcgtaa ctagctcacc
    23101 gtcttccttc gtacatactg catttgaatc aatgtagagc tttccatact tcattcgatt
    23161 ttctaattgt tgtttcttat gttttttcag aattgctagc aaggtttgtc caataaaaat
    23221 cgttcgattg gaactgcttg tctttggcgt accatatacc catgcgccgt cattctttac
    23281 catttgtttc tctacagtaa ttgttccatt tgaaaaatcg acatcatccc acgtcagacc
    23341 acaaacttcg ccaacgcgca ttcccgtata aaatccaata ttcaaaggaa tatagaaagg
    23401 atgtccttca ggagtaattt ctagcatatg atcaaagtcc tcaagagaaa tgatttttag
    23461 atctttttta gtcgttggtc gtgcttcata ttttggtatt tttacataca gcataggatt
    23521 ttgcttgatt aatccccaag gataaaccgc catattcagc gcattcttaa ggacagagtg
    23581 agtaatagtc attgttttct tcgagtaacc ctttttaaat tcagcattga tgaaattttg
    23641 taaaagagca ggggagagat ccgtaagttt tttctttcct aaataaccgt ttatatgatt
    23701 tttgatggta aatcggtagt tttcataggt attgtatttt agatttagtt taacgtattc
    23761 ctccatccaa aaatcaaggt attgttttac tcgagtatcc gtacctaaaa agtattgtcc
    23821 tgtttcgtca atatctgata aaacttttcg taaagcagct tcggcctctg gtcgggtgtc
    23881 tccgccaact ttctccactt tttttcttga gccatcatca ttgata
//
LOCUS       DACCCR010000037        23701 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCCR010000037 DACCCR010000000
VERSION     DACCCR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.039"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(254..847)
                     /locus_tag="IVK93_001511"
     CDS             complement(254..847)
                     /locus_tag="IVK93_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386806.1"
                     /translation="MRVKGQNSVDRKSILQFGKPEERSILDLFPYKSCTEEGVLITKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(848..1180)
                     /locus_tag="IVK93_001512"
     CDS             complement(848..1180)
                     /locus_tag="IVK93_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386807.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTIGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(1274..1744)
                     /locus_tag="IVK93_001513"
                     /pseudo
     CDS             complement(1274..1744)
                     /locus_tag="IVK93_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1805..4165)
                     /locus_tag="IVK93_001514"
     CDS             complement(1805..4165)
                     /locus_tag="IVK93_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386808.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQNV
                     FSWIYNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTTVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKNGLPKAQNLPMKDSRLNQ
                     FKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAP
                     EPIQKVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLKDIKP"
     gene            complement(4162..6771)
                     /locus_tag="IVK93_001515"
     CDS             complement(4162..6771)
                     /locus_tag="IVK93_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP6386809.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNTAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            complement(6771..8834)
                     /locus_tag="IVK93_001516"
     CDS             complement(6771..8834)
                     /locus_tag="IVK93_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386810.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGQLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            complement(8904..9206)
                     /locus_tag="IVK93_001517"
     CDS             complement(8904..9206)
                     /locus_tag="IVK93_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386811.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            complement(9224..9466)
                     /locus_tag="IVK93_001518"
     CDS             complement(9224..9466)
                     /locus_tag="IVK93_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386812.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            complement(9463..9927)
                     /locus_tag="IVK93_001519"
     CDS             complement(9463..9927)
                     /locus_tag="IVK93_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386813.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            complement(9954..12044)
                     /locus_tag="IVK93_001520"
     CDS             complement(9954..12044)
                     /locus_tag="IVK93_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6386814.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKTTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            complement(12041..12301)
                     /locus_tag="IVK93_001521"
     CDS             complement(12041..12301)
                     /locus_tag="IVK93_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386815.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            complement(12314..13075)
                     /locus_tag="IVK93_001522"
     CDS             complement(12314..13075)
                     /locus_tag="IVK93_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386816.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGKTKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(13091..13843)
                     /locus_tag="IVK93_001523"
     CDS             complement(13091..13843)
                     /locus_tag="IVK93_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6386817.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(13858..15834)
                     /locus_tag="IVK93_001524"
     CDS             complement(13858..15834)
                     /locus_tag="IVK93_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6386818.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(15887..16558)
                     /locus_tag="IVK93_001525"
     CDS             complement(15887..16558)
                     /locus_tag="IVK93_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6386819.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(16644..16838)
                     /locus_tag="IVK93_001526"
     CDS             complement(16644..16838)
                     /locus_tag="IVK93_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386820.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            complement(16950..17189)
                     /locus_tag="IVK93_001527"
     CDS             complement(16950..17189)
                     /locus_tag="IVK93_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386821.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(17303..17644)
                     /locus_tag="IVK93_001528"
     CDS             complement(17303..17644)
                     /locus_tag="IVK93_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP6386822.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(18263..18595)
                     /locus_tag="IVK93_001529"
     CDS             complement(18263..18595)
                     /locus_tag="IVK93_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP6386823.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(19442..20482)
                     /locus_tag="IVK93_001530"
     CDS             complement(19442..20482)
                     /locus_tag="IVK93_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6386824.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            22294..22512
                     /locus_tag="IVK93_001531"
                     /pseudo
     CDS             22294..22512
                     /locus_tag="IVK93_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            22772..23026
                     /locus_tag="IVK93_001532"
     CDS             22772..23026
                     /locus_tag="IVK93_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6386825.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            23027..23344
                     /locus_tag="IVK93_001533"
     CDS             23027..23344
                     /locus_tag="IVK93_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6386826.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT         6841 a         4545 c         3636 g         8679 t
ORIGIN      
        1 tttctatttc ccgatttcct agttgcttta atggatcaag taccttttga tcattttgtg
       61 gtgaaaaagc tgttttaatt gcttctaaca tgtacagtca cctcttcttg tttgattctt
      121 tacttggtta aaagattttc cttttcccaa cttacccagg cttaaagtgt gaggaaattt
      181 tctagtggtc tcttctttcg gaacatcttt ttctaaccat atcaacggat atttcattct
      241 ctcttcccca ttcctactca tcattcatat tattcattcg ataaagaatc ttgatcaatt
      301 cttttttgta gagtggtttt aaccctaaaa tgccactaga tcgcattaac tgacggtttc
      361 tggtttgcaa atccttgatt gtctttcctt caatgacgaa aaagaagctt aaatcagggc
      421 gttcctgttc tgctcgtttc aattcctgga tttcctctac taatttcttt tcatattttc
      481 ttaatcgctg tagatttctt cctaaggact gttccatacg atttttatct aataactgcc
      541 gcttttgcac gatttgctcc gttagatccg tttctgtggt caaactaact aacttaatcg
      601 ttggcacata cgtacgcatg acagtggtca actgattcat tcgttccagt ttctcttgtt
      661 catttaatcc ttccacatct gtagaaacta cacgataata acgttgaaaa tgatcttctt
      721 tagtaattaa gactccctct tctgtacagg atttataagg aaataagtcc aagatagagc
      781 gctcttctgg ttttccaaac tgcaaaatgg attttctgtc aactgaattt tgacctttga
      841 ctcgcattta ttcatcctcc ttatacgtac catgataatt tcggttagga tctaacgcat
      901 gataatggtt cttgtccgta aaaaaaatag actcgataat cgcaaaaatc tggcgttgac
      961 ctgggttatt atagggtcgc caaacaatca ctaaagctat cgcaatccat aagccataat
     1021 tcaagagtgc aaaaaagagt ggtaagtgga caagattatt gactattttc gcccaaaaaa
     1081 agcaaccacc aatcgttaat agatctaaaa agaaaaaggc gaaaaacttc cattccccac
     1141 taaatcttgg tggatttttc atatgaatgt atttatccat aatgtaacca tactccttat
     1201 gttctttatt tttagaaaca aaaaacactg tgatttctca cagcgttttt ctaaatatta
     1261 taactctaat tcaattttct tcttttaatt caaaaatttt ttgttctagg tttcttttca
     1321 tttgaatcaa ctcataattt tgtgcgcgta attcttcgtt ttccaaatga actcgttgca
     1381 tttcatcgtt taatcgattt aaattctcca atgattcttg tatcgtttct tgcctaaaat
     1441 taagtgagcc aagttgacga tctttttgtt gtctttgctc tttcaattct gacgatattt
     1501 gctcaacaga atgaaatagc tccgttaatt ttactgtgaa tgtgggcata gcatctttca
     1561 aataagtgaa tccttctgaa aggtgtacaa taatgctttc ggtatgtgtt ttttgaccat
     1621 ctaaaataga ctgatgatct ttttgtaaac tctccgtatc tttaaatcct ttaatagtcg
     1681 ccacaaatat cgttaaaaat gctcccagtg cagttataaa cgtagctatt aaaatatcct
     1741 ccacaatttt ctcctttcca ttttaaactt gttttataaa gaaaaaggcc cttaaatagg
     1801 ccttttatgg tttgatatcc ttcaatagtt gttctttcaa tgcattgttc ctgtttttgg
     1861 tatctcttgt cacatttatt tttgtcggtg cagtaaatgc cacatttgct ggtttgtaac
     1921 ctgtgtgaac tttttgaata ggttctggtg cagcttctct tgtcacaaca ttttgaacgg
     1981 acgcattccc tgtctcccca ctttcagata aattgtaaga aacagttgtc agatttccat
     2041 tttcaggcaa acgacttggt ttcatttgaa cattacctgc agttgattgg tgaacaaagc
     2101 tttgatcatt ctccttgaat tgattcaaac gactatcttt cattggtaaa ttttgtgcct
     2161 taggtaaacc atttttcgtt gatttttcag ttccaacttc gaccccatta tcatttggca
     2221 gatcgatgga atcatcatcc gtttgtactt cttgatttcg ttttggtaaa tctgaaatgc
     2281 gcttttcatt ttcagaatta atgttagatt tataacgata ttgttctgga tctgtttttg
     2341 gtaattgacc attttgtcct tcttctgaat tttcgttaga atgcaactca tcttggtgat
     2401 ctttggctga aggaatcgct gcaccttctt gattttcttt ttcggttgtt gtattagctt
     2461 ttactgctgc attgacttgt ccttcaccat acgcatcttt tagtccttct ttgacttctg
     2521 aagctttttc tttttgtttt tctagtgttt cggatggatt cttcacagct tcagccaatt
     2581 ctttagctct cgtgccgtct aatttctctt ctaaggtgtc tttggctttt tctgtaagat
     2641 cttcagctgt tccttttaac ccacgttcat tgacgtaacc aagaccttct gcggctttag
     2701 aaacaccatt tttcgctttc ccgaacattt cggaaactgt tttcttacta ttaggtacag
     2761 ttgtagcttt atcgtctgat tgttcttccc cgttatcagg ggtaacatct tttcgattac
     2821 caaatccatt tttacgaata tcatttaaat tgcttaaacc actcttacca gcacttgcaa
     2881 ggtttttagc cccgctaccc acatttttcg caatatttcc taaggcaaac atgctaagaa
     2941 gagaattgct gccgtttttc agactcgtat caacgccgaa aattttcgta actgattgag
     3001 agcctttgaa tagcgcaatg gttaatacaa ttaaagcgac aacatacaag aatccattga
     3061 tctttcctgc cgcatgcaaa tcaccgatac ctgaaagcat taatgtatag attcgtaatt
     3121 ctaaaatcgt aaatgccaat aacaagaagg attgagaaat atcttctata gcacttttca
     3181 tccgttgtcc tgtctcaaag tccgttgacg cggcaatcac taaatagaat tttttaaata
     3241 ccagctcaat aatgcaagta acagtcgtaa acaagataaa taaataggct accgacaaag
     3301 aagattcacc agctaaaata atacctggac tactaatgta tcggcgatag cctgaagtat
     3361 acacatattt tgctaaacct tcatcactaa tttgaacagg gatttctttc ccattatcgt
     3421 ctaactctag tcgatactgt aatgccttta accgatcttt ttgtactgta ctaatatcac
     3481 ttgattcaga cattttctta gcttgatcag gtgtaataat cccattgata ttcgctgttt
     3541 taaaattttt cgccgtgaga gcatttcttt tgtctgttgg tatgtgttct actttgttcg
     3601 gatcattttc tagtaacgta ctcacatcaa tcacattatt ttgaataatt tgaaaagcag
     3661 gtgaaccagt tttatctgct ttatttgctg cagtcacgtc tccgtaaaag tttttactaa
     3721 tatttagccc ttcatcgatt aaagaactac caccaaaaat caaaatcatg gccataaata
     3781 gattaaccaa gatatttttg atttttggtg gatgtttact cataaaaaat tttaagccta
     3841 aataaattaa tgtgacgaca caaaaaagag ccaatacctt actaatcaaa ctttgaaaca
     3901 gttcattaac accagcggcg ttcaataagt cttttaaatc aaagctttga taaattgttt
     3961 tttctagtaa agtattgaga ttatataacc ctttaatcaa accccataaa aaagtattat
     4021 aaatccaact gaatacattt tgatactgca gataattacc ataatcttgt aagactttcg
     4081 ttacggtttt gggatctgca tccccatccc caaataagcc tgaccaccag tcggcaatgc
     4141 cggaaaatat tcctccaatg atcattcttt caccgccttt tcagcttcta gcaaaagtcg
     4201 tttccttata acctctgtaa tttcttcatt ttgacgcaat ttttcaaggt attcttgcaa
     4261 acgatctatt tgcttctctt cataaaattg aataagtaac ttttgtgccg ttgagttgtc
     4321 taaagattga acgattttaa taaaaatgtt ttctttagct tcctcttcta tttgatcgtg
     4381 catattgtga taaagtcgac gtgcatagat atcaggtaga aaataatcgt aatcaatttg
     4441 taaagcctta agatccaagt ccgcatgtgt acaatgtatg tctatatcat tgatatccca
     4501 cgatggattg aaatcttcaa tatattgata agcataagga agattcgttt ccccatgatt
     4561 aaaaatagga tacggccgta ttttatgacc tttcaaatct tgtcgtttta aaggagcgag
     4621 tacaatcgtt tcaccttcta aaaaagtaga aattctttca ggagttaaga tgcgatctga
     4681 atcaacattg cgattgatat gagtattcat acctaatgaa ttgatggtac tgttctcttg
     4741 ttcaaccgtc ttattcccca cttttttgga aaattcttct atggtattat cgtctgtcga
     4801 cataatataa atatgtgtct gacaattttc tttgataatc gctgcgtttt gttccccata
     4861 cttcgcataa agttgctgat aggattggat aaacaaatcc cacaacatgt ttctaccagc
     4921 tgtgactgtc atgattcctt ccatattatc cattggtggc atattcccaa attcatctaa
     4981 aataaaatga atcctacggt gacatttatc tcctttagtc ttaacacatt gcgtagataa
     5041 ttctttatac aactgactaa gaaaaatact aacaatgata tggttagatg gatcataatc
     5101 aggtgtgagc ataaaaatag ccattggttt atcggtatag tgcatccgaa tagactctac
     5161 taataagtta cttttatcct catctaatgt tagaacggct tctttttcta attctggcaa
     5221 atgggtttct cctcgttttt ttcgataaac gatatttccc tcttcgtctt tcaatactct
     5281 ttgagaaaac tcataccaag caacatattc ttttcctcga tgatcgtatt ttaagcaaac
     5341 atgatctccc tgttctaaat gacaatcaaa gttaatttca caaaaacctc tatagcctac
     5401 tttgacacgt tcctcatgaa atttttttcc ttgatgataa aactctacaa caattcgctg
     5461 gtttaacaga ctctcgtgta gtttgagatc caaatacttc ggaaaaccaa tggacttata
     5521 atcaatggtt gattgagaag tcattttcgc aattttcgtc agtgtaaatg gatttagtcc
     5581 tgtgatcgct gtcgatagaa tgttgccttt cgctttatca gaaccaattt ttgttgcccc
     5641 atattgtttt tttgctgtgt ttccaggtgg taactgattc ataaattcat ctaaagcatt
     5701 gagctgcttg aattcattat caggatcttg gtaattaaaa ccaccaatct cagaaagaag
     5761 ttcgacgaga ttattcggtg ttaatttgga gtaatcccct gtctgatccg cttgttccaa
     5821 taacgcaaaa ataagcgcat tgacagcttt ttgcgcattt tcgttaaagt atttattgtc
     5881 tgaagcgttg gcatcataat aaatcgtatt cgtaaaagta ttggttaact gcgttgcagt
     5941 atctatgtct cctcgcttcc agtatttcac gatgagtgcc aatggattat cactcattga
     6001 ttgcagtgga tcggtgatat taaatgcata cacatcatac cctctagctt ctagttcatc
     6061 tttactagca acatataatt ctgtttttgg atcattaaca accattgaac tttgtctttc
     6121 tgcacgagac aaattgtcaa tggttgctaa aacttctatc tgtcctttcc ctgaacggga
     6181 agtaccaaca atacaagtat ttactgtatc cgtctgaata taccagtact ttttataatg
     6241 agtaattggt attccgccat aaccaggaaa acctcccttt gattctggta tttgcggata
     6301 aatttgttgg atttccttta acgtttttaa tcggttatct cctttttgcc catctgctag
     6361 aggacgaaac cacatgtcca ttttcaatcc cataagtaca gctggaacaa cacaaatcgt
     6421 agaaacaact aaacgtgtgc ctaaacttgt tgttggggtc aaaggattcc aaaaaccagt
     6481 tgaatcaaaa tggaataact gcggcatttc ttgtaactga aaaatattca ctccttgtaa
     6541 acttcctgga ataatctcaa atcgatgaaa tgcttctggt attcttgtaa caacatagtt
     6601 taaaggaaga taagctaagc cactataaaa aatcttttga gaaatacgtt tgtcatgttt
     6661 ttctggtagc tcttctcctg aaggagtata gcgatagaaa tgagaaagat acctattctt
     6721 ttctaaattg aatcgtttgt cttttcgtct cgttgtttct ctaacacgca ttatttcacc
     6781 tcatttgatt ctgaactttc ggaagtgttt gattgtacag ttgaatcgct tgatccttct
     6841 ttagccttac taggatcact ctctttgcta ttttccatac ttgattctgt tgttgactga
     6901 ttggtacgat tcaataattc ttctaattgt tgttcatact cttttcttgc ttgttcttgg
     6961 gcttcttcct tgattgacgt cgttgtggcc acagaatcag cctgtgaaga ttgattgata
     7021 tgattaaaag caaatactgt tcctccacct agaagcaagc ttaaacttgc aatacatccc
     7081 accatccatc cgttaaagga acgctgtggt gaaggagtta gatttgtttg gttgttcatt
     7141 ggggtttgaa cagcagctac tgaaggcgtt ggttgaggtt gtgctagata atttaactga
     7201 gcttgtcgca atgtatccat ttgttcttga cgttccttac gagcagctgc ttctttttcc
     7261 ttttcttggt ttaaccgttg tttttctatt gccatttgtt gttccgcaag atcctgttca
     7321 cgttttttta gttctaattg ttcgcgctct ttttcttttt gccgttgttt ttcttcttgt
     7381 tctttcaatt cttgttgttg aatttcatac tcttttttcc attttggaat ttgcacttgt
     7441 aattcttgct ggatcttttc gtgtttttcc ataagaactt cagtcgttgt ttgatatagc
     7501 ttattcagtt gttcatttaa ttctttgcta atctttagct tatctgtacg caaacctcgg
     7561 taaaattctt cttttcgttc tttaatacgt tcttttaaca agttttcttt ttctttcgat
     7621 aacgcttctt gattcgtctg agtaaaatta ttgatctttg ctacacacaa ggaatacgca
     7681 ttgtcgcgtt tcttttcgta tcgctcttta attttttgta cttcttcttc aatcactcgc
     7741 tgttcttctt tatttagttc ctcttttgct ttctcccaat tagacgactg ttccgtcgca
     7801 tactcattaa tttcttgatc tgtttcttgt tgacgttctt caaaggtggc taataaatct
     7861 tcttgtgcaa gctcatgcca atttgttgat tggacagact ctaactgttc taaaagtgtg
     7921 cctttaccct gtgagattaa ttcttcttta ctctctaaaa atgcttgata ggtttggggt
     7981 aattgatatt tcgaataaag ctcatttaaa aataatttgt ttttttcttt catccattca
     8041 tgctctggcg gatattgatc caaaatataa tcaaatacag ttgccttttg tatctctatt
     8101 tcgttttttt ctacttcttc actgataagt ggttcaatat attcctgaga atccccttca
     8161 gtccatgttg actcttcatt cgcttcaact ggatcaactt ctacttcggc cgtctcttca
     8221 tcctccattg tttcttcaaa gggatctacc tccaattgac ccatcacatc gttctcttcc
     8281 tcaaatatcg atttctgagt cggaatatca gcttgttcgt caacatagac tgtctctttt
     8341 tcttttgact ccttctttgg aaatattggt cgttcccatg gtacttcttc atttacttcg
     8401 acttcactcc cttgattcac ttttctttgg tcgctctcta actcttcttc taaaacttgt
     8461 aaaagttttt cgtttcgata agactcttcc acactcatag tttcatccgt ttctttaaac
     8521 acatcaaggc ggattaaatc catcaatgta ggatagaaac cttctgaaat ttgatagtcc
     8581 agttgcccaa aaacgtattc tgtaccagct ttctctattt ttccttctgt gtcatgtaac
     8641 gtgagagtca acttaacaga agatcgactt tcgtgttcca gtactttccc taacacatgt
     8701 agctgttctt ccgtttcaat gacagaatat ttaattgttt tcggtaaaaa agaagtacgc
     8761 tttcctactt ccttcgttgc tttttttatt aactctttat ctgttttggt attgcctgca
     8821 atcgtaaagt acatgagtat tctcctttca taaaaaagga gtaaaaagta tcgactactt
     8881 cttactccta cttttttact ttcttataat ccttgaggtg tttgtccaaa caatccaaat
     8941 aacgaaacca caatagaagc tgctgctaaa ataactccta cccccatcaa tacatagcct
     9001 acgtgcatct tcccattttc agctcctctt cgtccaaata ggaagaaaag acccccaata
     9061 acaatggaag caacggccgc agcagcagca aaaatcatca gattgttttt taaactagtt
     9121 gcggcagttt gtaatgctct aaaaatatca accgcagcta ctactttcgg ttgtgttaaa
     9181 aaattcattg taattgattc aaacataaaa ttgttcgctc cttttagtgt ttaataattt
     9241 cgtcgaaaac aagcttttca cgttcacgtt gatagatata tccttctaag tccttgtctt
     9301 ttccccgtaa taggtcaaat tgtgcttttt caaggacttc ttgtttccac tgtgtttcag
     9361 atacaccgcg gcgttctaag attttagacg cggctttttc aaatggcgtt ttagcttttg
     9421 ccactgtgat tcctccttca ggacaaataa aaagactagc aatcactcgc tagtcttttg
     9481 taacttatat ttattggatt gtgatccatc gtgaatcaat ttctctttcg ttcgcttttc
     9541 atccttgcta gatccaagaa aaattatttt ttttacgcca ttcttctcat agggaacaac
     9601 aataaataaa gaatcagatt tgtttataaa ttccctaaca ccagctgaat ctactatttt
     9661 gtatctttct aaacctttat agtctttttg cctcttgact gttatttgta caggatctgg
     9721 atgccataca ctctcagagt attcccaggt agtctcgtct ataattttca aattataaac
     9781 aagaccacct acttcttctt tccacatagt tcctgatttc aatacgccat tatcttgatt
     9841 agtttgttta tttttttcat tactacatcc gactaaaata acaatcatgg caatcaagga
     9901 agtaactact attattaact ttttcatttt attcattctt tcgttttttt attttaaatg
     9961 tatctcagaa ccgcataact aaaagttgct aacacaagta atagtaagct gaccgctgaa
    10021 atcgttacta aatgtgtaga acctgtttta ggaacaatga cctgtggcaa gttaggatca
    10081 accccaattg tcacaacatt tgttttatta gaaacattat ctactgctac tacggcttgg
    10141 ttatcaatat ttttttctga ttgtaatgga gttttagctg taaatatcag ctgaatttct
    10201 cgtccatcta aaagattgtt acttaagaag tcagcggaag cttcccactt cagcgttctt
    10261 gaattgctat cataggttag tgtgccttca gaagtaatat cttttccttt actcttatct
    10321 attacttgcg cagaaccttc tttatatgtc aaacgagctt caattggatc ttgaatacta
    10381 aattgtttgt agtgatctga cgcagcgtct acgccttttg tgtgccattt ttgtgtgact
    10441 agatattgta cttcagatcc tgcgtttaaa ggagctgtcg gcgtaccctt cttaccgtca
    10501 tttcgtccgt ttacatcttt tccattttgt tgtacttctt tcactggaat tttagggaaa
    10561 taaacggtaa tgatatttgt atccttttct actttattaa tgatcgacgt tccctgattt
    10621 ttggctacac tattttgatt ggccaattca ttgactttta cattgatcac taattcgtaa
    10681 gtttcgccgt tatacttcat tcgatttttt aatgtatcag ctttcgctgc atattttacc
    10741 tggtgttttg ttttgtcata ggtcacttca ccagatacat cgaatttttt atttgtacca
    10801 ctttcaacgt gtgcatcgac gtaatttact tcttttggta aattatcgat taactcaaat
    10861 cgatcgtaaa ccgctagtaa atccactcct aatcgattca ccttctgttt gattcgatat
    10921 tgaatcgtgt ctcctacttg caacatttta tcgttcacgt tatttccatt tttatcaacc
    10981 cctgtttttt caggttgatc aggaacaaca ctaaagattg tcgcagaaga taaagtaaac
    11041 caggtttgaa cattagaaga accaatcaca aacgtcggat ttgcttcaga tattttaaag
    11101 gacacagtac cttgaccaaa cttcggatct cctaatttat catgcaaatt attgaaatca
    11161 ttatccgctt gtgtcagctt agagcttcct cctgggtggc cacctaccac gttcagattg
    11221 ttgtagaaag atttaaattc tgaaagaacc gtatcttttc tgacatacca atctgtcgta
    11281 ttcattttgt catagtgggc atattctcct ttaagatccg tggtttttcc tcctgcaggg
    11341 ttcaaagagt taaaactgat aaatgtatct cctgaaaatt gaacggggct accgtcctca
    11401 tatgtggcta ctagctgcac attcatttgt gttgaatggt aaacaattcc tgagaaaaga
    11461 ttgtctgaga tccaaagtac tgttcttttt ttagatttac tcattgcacc attcgtattt
    11521 ttatagccat cgtctcgaaa atgagttttt gtatagttgt tatctagtag attccacggc
    11581 aacgtatccg agtataagtg aatattagat agaactagct tgatattgac ttttttcccg
    11641 ttatacgtac cgatttttcc atagtcaata ttgactttgg tgttggttgt tacgccttct
    11701 aaaacaaggt ttgtttcacc gtattctttt tggaaagaag gatagcgtat atttttgttt
    11761 ccctccacta atcctacgta gtcgtctgta aaaccacctt tgttggcatc aacatgatat
    11821 ttgtaaactt ttgacgctcc gtttacagta atatttgttt ttgcattagg aacatctttt
    11881 ggaataataa aattattctt tacgttctgg attggatttg acggatcgac aataactttt
    11941 tcagcaaaga cattggctgt ttttaaccca aacaatgtac ctaacatcat cattatggga
    12001 aaaacatatt ttaaccgtct tgtccacgag tgtttaaaat tcatcccttc atcccctctt
    12061 ttttataaat gactagtgaa attccgacaa taacgatcaa attgatcgta gtaaataatg
    12121 gattcctaat gagcttttcc ataatatcta gcggcatgga agcagctggt atgtttaaaa
    12181 tcctcattag gtatagttgg tagcttggca aaatagatag tttagaaaat aataaaccgc
    12241 agtacaaaaa gactgcggtt aaacaagaaa aattgaataa taataatttg atcagtttca
    12301 ttttatttct tccttaatct ctagaaaaca agtgagtaac aaaatcccaa caattctcaa
    12361 agaagttttt ctttacgcca gtagaaacga ttttttttac ttcttcttta ggttgcgaag
    12421 cttctgattt aggatagatc cacacatcct ttaagaattc accgttttta tcagaaacag
    12481 gtgttaatag caaaatatcc tcgctattag aaatgttttc aggacttgat tcattgacaa
    12541 tataataagc ttgtttctga tttgcctgta cttctatcga gaattcaaag acacctgttt
    12601 gttgatcaat tgtctttgtt tttccattag aaactagctt caattgatca ctgaatagtt
    12661 ttttcactcg atcttttaat tgggattcaa tttggtcagt agtgagatcc ttaacatcca
    12721 tcttttgtaa aatgtccgat acatcataaa cagaaaactt cgcgccattt actccttctt
    12781 gtttttcaag tttttgcccc tggttgtcta attgttcgga agaaataatt ttttgaacgt
    12841 gaatggtctg tttgactatt tgatcgtctt cagcaatttt cggttcttct gaagaatcaa
    12901 ctgttgacga atcgacggtt tcatcgctgc tttgaatcgt gctgctatct gacgtttgga
    12961 ttgtttgttc tgtgctatcc gtgctttctt gtctgtctgc aaacgtcaac tgtgtgtagg
    13021 cagtgaaaga aaaaagagcg gctgcacaaa caaatagcca ttttctattt ctcatgttgg
    13081 ttaccctcct ttattttttc tttttataaa caaatagcga accaatgatc atgactgaaa
    13141 gaaaaacagt acttcctaaa agccatgtcc aagataattt ttgtttctta ttttcttcat
    13201 cttttaattc tgattttgta aaggttactc gcttcccata cactagaaaa cggtgtgtgt
    13261 tgatcccctt aggcgtacat gtcattagcg ttactcgatc ttcattggga tttgattcta
    13321 agtaagtgac aaagtttctt tgtagttcgt ctttttgcac ttcctcaatc cgatcgattt
    13381 gataagcgtg ctgctcacca tcaactttga tataaaactt ttctcccttt tttactgaag
    13441 gtaaatcatc aaaaagatta tctttataaa gtccactatg acctgcgatt aaaggatttt
    13501 taccgtttcc tcctgtaata tcaccagttc cttccgtaat acctacacca ttttctaaaa
    13561 ttttgtcgga cgttccgtca taaattggta gttttaaaga aatacttaga attgtaagaa
    13621 taccaacagg atcgcctaac tcaatcttag caacatccaa agaagaatcc gtttctgctg
    13681 ccgttcctgt tcgatcgatt gcggcaatct gctgattttg ttggtatttc gccacgctcg
    13741 cttcagcagg catcaaaagt aagacaccta cagctgaaag taaccagaaa gcaatgatcc
    13801 atttacccat gattcgcttt ttcttaggct tcctgataat cattggtttt gatccttcta
    13861 ggctttcttc ttaaagtaaa caactgcaga aagtgcccct gctcctagca acaagcaaga
    13921 gattaataca tacgtttgaa aaccgttgct accggtcatt ggtacggaag ctttttgatt
    13981 gtttttgatc gtgattcggt tttcttcttt gtatgaatct ttgtctactt tgaaagagat
    14041 ctctttcgtt aatttttgat agttttcagg cgcttttgtt tcttccagtg aatattcacc
    14101 ttcagttaaa ccagtgattt caaagtgacc ttctttatct gattttagga tcgctgcatt
    14161 tgctacatcg tctacccata ctgcttcttt gacaccgtct ttatccgtat caactaatcc
    14221 accataatat tttccttctg cgtttttagc agcaaattct gcgccttcaa gcggtgttgc
    14281 tttgattcct gttccaaata gtccgctatc gacttttaag aattttgcac cataagtcac
    14341 aacgtcagct ttcgtttttt cgtggtgttc ttcctgatta aatggattgt tgttataaac
    14401 gaaatccacg ctatttgtta atggattggc aacagctgcg ctgtcattta catacatgtt
    14461 ataagtcatt tgaagatctt gtccgtgtaa acttgctaca gtatcactta cttggccatt
    14521 attcacgata aaatcgatca caaagccatt ttctgaattt tggattttat aatctgtatt
    14581 ttcttttaat accacagatc catctttatt tgttactttg atatttgctt tagcagcgac
    14641 ataagaaagt ccttgtcctg ctgtatcccc ccatttaaat acactgtaag gagaagtttc
    14701 gcttttcact ccgtttactg tgataccgtc tgctccgcct gcaatgtctg ttgggatatg
    14761 aatcgttcct tgataattta ctgcgtcacc aatttggaaa gaatgttctt gtcctgttcc
    14821 atttgttact gttttatcga tcactggtgc tttatagttt acgtatgaag cttttaagtc
    14881 acttgtcacg ccatcattcg taatttcaaa ggttaattta ttgtgcgcat cgttgcgagc
    14941 gtctgcgcct aataaatatt gatcggctgt tggttcttta tctgatccca ctacaacttc
    15001 tgaaggaact tccacaaaat agtattttcc taacgtcaaa tcagtagcag tcagctttcc
    15061 ttgatcatcc gatacaagat ctcctaattt tgttcctttt ccaggttcgc ccttgtataa
    15121 ctcaaatttt gcccctttta aaggtgtttg tttgctttga gctgtgccat cgtcaccaaa
    15181 tttcgttaat tcaaaactca acttagaaac aatattttta ggatataagt gaatgtcttt
    15241 taaaaaacct gaacctgtat tatcagtcat tggcgcaatc acaaccattg gttttgcttt
    15301 ttgtgtgact aatccagcag cggatttact ttcataaacc acatatacat tccctttgga
    15361 attgacatag gctggttgat cagcaaaagt gatctcacca ttttcatcta cagctgaagt
    15421 aattttgttg gttgcattct tgacgtactc actgttagat cccttgtctt ctacatcttt
    15481 aacgatttcg tccaccttcg cgttggttag atcagaatct tcagtcggta atacttgatc
    15541 cgtaatattt cctaacgtaa actcaacatc accataatct gccttgttgt atttcgttac
    15601 acctgtagga tattgatcgt gcgtataacc atcgtttttg atcccgtcaa tatcaacatt
    15661 caattgcgtt ccttgatcgt acattaattt atgaaccgtg atattgacct tctctggtgg
    15721 cgttttttgc gtgacatctt ctgcatatgc tcgatatccc tgaaagcagc ctgcaatgat
    15781 tcctaacatt ccaataagca ttccagcttt ttttacatac cgatttattg ccatttttta
    15841 ctctcctcat ttcatatttc aacgactcac gttgattaaa accttgttat tgtagttctc
    15901 cctgtaccac gtatctttct tttccttgtg acgtataaac acaagtaaac aacgttaata
    15961 agggtttctc tcctttcttt ggcaaagaca agaaagagtt ctcttcagga acgccatcga
    16021 ttaatttaaa gtactcaggt acgataaatt tacgcgttac cttgtaacga tagacgccat
    16081 cgttactaac taagtccatc acctccccta atgacaactt ttgaatatca gaaaataaaa
    16141 cacctggcat ttccatgtta tgacctgcta ggacatagtt tcctttgccc atttcagcat
    16201 cttcataaaa ataagtagcc gctccaagac tcaaagtgta ttgatttgcc cctttaaaaa
    16261 ttggcaatgc taaaccaagt gcaggaatct gaatttgtgc aaatacttgt ttattgacgc
    16321 ccatttcgat tgcttcttgt tgtatggaca acatttgccc caaagacata tcattattga
    16381 cgttcgttgc atcctcctta taactctttg catgtgcatt gatttgatct acgggcatct
    16441 ttttttctat attttgctta aacacctctc cttcatgttt ttggtagcta acatagccaa
    16501 acacactgca aaagacgatt aaagcgactc ctaaaaaacc aaataaccat tttctcatcc
    16561 agctaccccc tttcagacaa aacaaaaaat ctttttctca tttccataga aaaagtttta
    16621 aaaatgaatt ctattaatga atgtcatgag acttctaaca atagttgaca attagaactt
    16681 gttttcacat aagttcgtct caactgttca aagtttttca ttgaacctaa caaacaagct
    16741 ttctcttttc ctgttatctg catatcattg atatattgca tgacagataa tcttttaagg
    16801 tgtttttgtt tcatcgtttt tagcaacttt tcttccattt atttcgacac tttttttctg
    16861 ttttcgattt cttcataatt catgagtttt cctaccttcc ttgttttttg caaaaataaa
    16921 aaagcctatg atcacatacg ctctacactt cagtcttggg ttatcttttt ctttcgttta
    16981 attactggcc gtgctggttt caaatccttg attccattat gggttctcca ataatcgaat
    17041 tcttttcgta atctttcttt ttccatatct gttcctttag acagggattt gaatacatct
    17101 ttaggtgaat aaccacgagc taacagctgt acataccttc cttttgtcaa agtatccata
    17161 tccatcgcct ctaaaactgg tcgcgccatc taattttcct cctaaatgaa ttccttactc
    17221 aatgcctaag gtaatactac ctttttatta ttcgtcgtga cttcttctaa gcctttatct
    17281 gtaaaattta ttcctccagt ttttattctt ctttatccgc tacaaattca atgatttcaa
    17341 cgctgacaat ataatcactc aaatatgacc aagtatcttc aacagataat tcgtctccat
    17401 tatcattcgt aagcgtcatt ttaaaccatt cattaccttc atcatcttca ggtagcaagc
    17461 cgaatccaat tggattatca gtcaattttt gatagtataa atcatcctcg tctaatgcta
    17521 aaacatctaa tcctttacaa tttccgcctc ttctaaacgt cacaaatcct ttgtaatccc
    17581 aacgttggaa agaaacctta atagtatgtg tgcccaatcg tatattcgga tcataatttt
    17641 tcatttgtca ttcctccatt acgtaaattt cattttctct atgttttata tcaatttttt
    17701 cactttattc aggactcgtt catattttat cgtttttcgt ttacttaaaa atacataaaa
    17761 aatatcgact cgtttatggc gtccgaaacg agaaacccta gtagaaatcg gtaaatgctc
    17821 gattgatcca atgaccaacg tcctcgcaag tatctacatg tactgcagca cacgacacaa
    17881 tttcttgtgc gtcctcccca ttacgagaag acctactcct tattccatga gtttgcgtca
    17941 aagcctagat cccttagtag ctgctcatga ttaatctagg cgctattcta acttgtctaa
    18001 cttctaaatt ccctcttgtt tactctgtgc atattattta cacaaattca tttcaccact
    18061 atccaaacaa gggcatttac tcgattgacc aattttttac gttaccaatc gtaaaaacct
    18121 accccgtcac cccccgaaag gaattgttga tagatcaagt ctactcctta tttttctagt
    18181 aattctaagt tcttttagaa gtctcgacaa tattttcaag caagcgaaaa acgataaaat
    18241 acaagttatg agaatgatca attcagctga ttaaacgccc catccctttt gcggtacaac
    18301 gcaagactcc attttttctt cgaccaatca gtacaccgtt ttggtcataa acatgtttga
    18361 ttgttggata tagttctttt aaagggatag gatcttttaa aattatttgc ttttgtttac
    18421 cagcgtaata aatcatttcc tctgagactt tgtctttctg gattatatat ttcttacact
    18481 gcacaacggt tttactttgg ccagttgata gtttcttcgt gattttctct gtagcggtac
    18541 accgtttacc gtagtgacaa atatcttcga gactcaaaat tcctgtatat gacataaaat
    18601 aatccaccca cttactttaa atatttttaa taagtttttc ttgttatttt tcaaaaatag
    18661 agaattgatt ttcgttcatg acaaacaaaa aagagggaac gcatccaaac atgcgttccc
    18721 tctaatttca gcgttcgtca tacggcaaag agccacacgg acatttttat aacattatgc
    18781 ttaggcatag attcatcgta atatcacgta tttttaacct taacacagcc aaaaaaagtg
    18841 gtatcacttt tgaatatctg ttatactaaa tccgttgcgg gtaataccgt aacactgaaa
    18901 ggtaaacgca ttaagggatt aaaggttgtc ttaatgccac ctgtggacgt cgacgaaggt
    18961 taccagcctt ccgagtctca cagggccttt cttttttttg tattttcaat tgtccaaatt
    19021 tatcgtccaa aagtgcagaa cgaaaattaa ttcatcacaa gcgtagcaga caattaaatt
    19081 taaaacaact aaatttatac aaaaaaatgt aagcgaataa tattcgtatt gcctgaaaag
    19141 aagtaatatc aaggttttta gcttttttaa aaaatgaaat tacctctctt cgcgtattat
    19201 tataactaaa aaacagcaac atgcaaacgt ttgttctctt ttttgtgttg gaaattaatt
    19261 attaactatt aacacttatg acgtaaaaag ctacgtttgt gatttttatc ataatatttt
    19321 aactcataaa ttctattaaa atagctattt ctttgtaaca tttgtaatat aattagaata
    19381 ataaaaaatt tttaatttta atgctactcc atgaagcaac aatcaatttg tcagtgaaat
    19441 ttcactctaa ccagtctttc gtaatagttt ttgggaagct ttcagaattc aaacgttttt
    19501 cacttgatac tttagcagct attggttttt cgataaacca attttttaca tagacaaaaa
    19561 tcaggttctg gatattttca actttttcgg tttttaattt ttgataggct tttaacagtg
    19621 tcgtgtataa ttcttgttct gcattgacat aataactatc gagttcttca aaaattattg
    19681 aaataccact ttctgtttcg gcttttttct ttgcattatg aatagtctgg acagtttttt
    19741 gagcttcact gtaacttgaa gaaaatgttt ttattaaatt taatactcgt tcagggataa
    19801 acgtagcaat gctgtcgttt gtcataattg tctcaaaatt gtctaacaac aattgatctt
    19861 gtaggcggtc tttttcagtg tctatattgt gtctattagt gtctaatata ttattattaa
    19921 gttctggtat gattttcata ccctctgatt tatcaacgtt ttggcacggt atgattttca
    19981 taccctcttg agccccaagg gattcagcgt ctgatttttc gtcgagggtg ggtatgattt
    20041 tcataccctc tgatttatca acgttttccc tattctctct atcaaatttt tcgagtaagt
    20101 agatatcatt ttctgagact tttaattctg ctagatatag tctattagga ttatttttcc
    20161 ctaactgttt gttaaatccc atctgctttt gtaataataa accaaaggat tccaactctt
    20221 ttttgattgc tatcgctttt tgtttttggc agtttaatac gctacatagt tcatttgtcg
    20281 tataagtaaa ataaacatta ccttcttcat cgatttgccg tttgctgatt gcgatctcta
    20341 atcttgcttt taaaagcata taggcaatct tagttgaatc tttcattttt ttgtattcgt
    20401 cagaaacaat aaagactttt ggaaattgat aaaataattc gttgtaaact ctatccgcct
    20461 taaaaaaatt aaaatcagtc attgtagatc ctctactttc acgttgtttt atttatgtaa
    20521 aattacctac tcttttcgaa aatagcaaag ataaataaaa aatgcaaacg cattaaaact
    20581 gttttttaat taaatactac ctaaaatcag aaataaaatg aattatgtac tacctttaaa
    20641 gtcattaaga actactttaa ataaatatta tttataatta aatactacca gaagaacgta
    20701 tgaataaaat ttagttttaa aattaaagat aattaagtac taccagtgtc aaaacaaaaa
    20761 ataaattaag aactacctta tgcatataaa acatactacc attactagta atatatacta
    20821 ccttatttca gtagtatata ttatattctt cttataagac aaggctttag gctagaaaac
    20881 aatagattat atactactaa aaaatacaat aagtactacc aatgtcagat atatataata
    20941 attatgtact accattaaaa ttaacttatt taataagata ctactgtgtt aatctgaaaa
    21001 aaattacttt aaagtagtta catactacct gttaaattag atactaccat taatacttaa
    21061 attagatact acctatattt atattaagta ctaccgttct aggaataaga taataaaaaa
    21121 aaactaccaa attgcaatta aatactactt aaaatgtaaa aaataagaac tacccaaatt
    21181 aggtagttct taataagaga atatcagata aatttgtaca agtcataaaa aaattaatct
    21241 taaattttta aatatatata cctgttaatt ataaactact ttatttaggt agtacataat
    21301 taacagataa aactacatgc ttgtagtttg tctagctaat gagttgcata tgatttatat
    21361 aaaggtgtgc agcataactt agttgaagtt aagagaatag aataaatatt ttttgttttg
    21421 ctaataatta aacactacct aaatatggta gtgtttaatt atataatatt cattttgtta
    21481 caaataatcg tattataaaa gcataacaat tgggataact attttttaga ttatttaagt
    21541 actacttaaa ttaggtagta cttaaatttt ggtttaaggt agtaggtaat taagaaataa
    21601 tatatatctt cttttataat agaaggggag aggcctagtt aaaaacagcg acaattaata
    21661 acaatgtatt taatgcttca caaaatccat ttggtggaca atgcattgtt attagcgatg
    21721 atctaccgtt ctaagtagtt ttttctattg gttgcattcg tgactaatta tggtataatt
    21781 atgagtaaca aagaggtatt tcattatatc atcaaacccc atcagcgtgt aggctgatgg
    21841 ggtttttttt acgtttctat attatagaat taactggtag gcttactgtc ttttttggca
    21901 attttgcaat caagccactt gcagatgtag tgtgcagtta cctctgcaaa tggcttgatt
    21961 gcaaaaacaa aatcaaaaag gtatttcatt atatctcctc ctccccttgc cagattaggc
    22021 cggggtagca gacagcaaaa ctattataac ataactgaaa tacctaaatg acatatacag
    22081 atatgataat ttatgtattg atttttctaa caaaagcaca tattttaata tatactacct
    22141 attttaggta gtatatattt ttagatacaa tatcgatcag ttttgaggaa atgaaattct
    22201 agatattttt tgctacatag agttactttc aatatttttt aacgtaatta ggcagaagtc
    22261 actcatcctc tataggtttg tgatgaaatt gctattgatc catttaagta agagcaaaaa
    22321 actggaggtt tatcaggtaa ttggtctaat ggatttaagt atgcggtcac cgtgtataga
    22381 gttgcttatg agattcaaga aaataaagtt attccagtat tgctatatgg aacacataac
    22441 aatttttatg aattagccga gaacactcgc aactttattc gtgtagatga atcggcttcg
    22501 gttgagttat aactttagct atatcaatca accgaccgac atatgttaaa tctatatata
    22561 ataaaaatac ctacttaaat ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat
    22621 tttgatatcg tttgtatatg ttttcagcgt tctaagtggc ttattttgtc caataataat
    22681 atggagtgga atcccttaag aaacaccgtt ggcaattgtc gttataacat gttataattt
    22741 atttatcaaa aactaaaaga agtgtgataa catgggaaaa gaactaaagg ttagaaagat
    22801 cggcaatagc gtgggagtga tccttccaag ctctcttgga ttaaaaagcg gagacactat
    22861 ccaagccaaa caagaaggca acttattcat attagatact actcaaatag caaaagaaca
    22921 cgatcgcaaa ttgattgaag aaagtttcca agactttgag aaaggtttaa cagtttcaga
    22981 aattgaaatg gtaaaagctt tcggaaaata cggctggagt gaatagatga aagagtacac
    23041 gctagtttat tctaaaagtt tccagaccag ccttgaaacg ataatagaag aatgggaaaa
    23101 tgagcttgct ctttctccag ccagcataaa aagatttgta ggtgcgatcc agaaggcttt
    23161 agaactaacc aaaaattttc caactatgca tgaggaagtt tccgaaatct atggaatgga
    23221 tatcccaaca tatcggatcg tgatcggcaa aagctatgcc ttattttatc ggatcgacca
    23281 tgaaaaaaaa gagatacttg taggacgtat tttccaatct aaacaaatga aactaggctt
    23341 ttaacaataa ttaggcaaaa gtcgctcatc ctctacaggt gggtgattaa tgccgctcga
    23401 tatggttgtt aaccgtcggt tactcgaaga ttgatatata agacttagta cctttttatt
    23461 ttgctccatg ttatggccga aggtctaaag atacaatcat tagtctagag aaaaattaag
    23521 aaccgtagga actacgggga tcgcttggtg aataagagac actgctatta gtgaaaaaat
    23581 aagatatcaa gtacgctcta ttcccaaaaa tttcgtgact ttactcatga gaggttcaag
    23641 tagtatattt ttaatcaaaa ttgacgtaac ttttaataaa tactacctaa ataaggtagt
    23701 a
//
LOCUS       DACCCR010000038        23571 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCCR010000038 DACCCR010000000
VERSION     DACCCR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.040"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVK93_001534"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVK93_001534"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP6386827.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IVK93_001535"
     CDS             complement(1172..1867)
                     /locus_tag="IVK93_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP6386828.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IVK93_001536"
     CDS             complement(1880..2596)
                     /locus_tag="IVK93_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP6386829.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="IVK93_001537"
     CDS             2926..3531
                     /locus_tag="IVK93_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP6386830.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="IVK93_001538"
     CDS             3856..4179
                     /locus_tag="IVK93_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP6386831.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="IVK93_001539"
     CDS             4361..5731
                     /locus_tag="IVK93_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP6386832.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="IVK93_001540"
     CDS             complement(5861..7162)
                     /locus_tag="IVK93_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6386833.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="IVK93_001541"
     CDS             complement(7392..9383)
                     /locus_tag="IVK93_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP6386834.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="IVK93_001542"
     CDS             complement(9527..9793)
                     /locus_tag="IVK93_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6386835.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IVK93_001543"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IVK93_001543"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP6386836.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IVK93_001544"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IVK93_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP6386837.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="IVK93_001545"
     CDS             13137..13700
                     /locus_tag="IVK93_001545"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6386838.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="IVK93_001546"
     CDS             complement(13953..14474)
                     /locus_tag="IVK93_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP6386839.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="IVK93_001547"
     CDS             complement(14477..15319)
                     /locus_tag="IVK93_001547"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP6386840.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="IVK93_001548"
     CDS             complement(15312..16037)
                     /locus_tag="IVK93_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP6386841.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="IVK93_001549"
     CDS             16213..16509
                     /locus_tag="IVK93_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386842.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="IVK93_001550"
     CDS             17077..18669
                     /locus_tag="IVK93_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6386843.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="IVK93_001551"
     CDS             18682..19422
                     /locus_tag="IVK93_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6386844.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="IVK93_001552"
     CDS             19590..20939
                     /locus_tag="IVK93_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6386845.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="IVK93_001553"
     CDS             complement(21067..21657)
                     /locus_tag="IVK93_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6386846.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="IVK93_001554"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="IVK93_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6386847.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DACCCR010000039        23347 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCCR010000039 DACCCR010000000
VERSION     DACCCR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.041"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(10..2154)
                     /locus_tag="IVK93_001555"
     CDS             complement(10..2154)
                     /locus_tag="IVK93_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6386848.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2511..3884)
                     /locus_tag="IVK93_001556"
     CDS             complement(2511..3884)
                     /locus_tag="IVK93_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6386849.1"
                     /translation="METTMNTEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPIMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3884..4279)
                     /gene="rbsD"
                     /locus_tag="IVK93_001557"
     CDS             complement(3884..4279)
                     /gene="rbsD"
                     /locus_tag="IVK93_001557"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP6386850.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGSEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4307..5224)
                     /gene="rbsK"
                     /locus_tag="IVK93_001558"
     CDS             complement(4307..5224)
                     /gene="rbsK"
                     /locus_tag="IVK93_001558"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP6386851.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5333..6346)
                     /locus_tag="IVK93_001559"
     CDS             complement(5333..6346)
                     /locus_tag="IVK93_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6386852.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6427..7029)
                     /locus_tag="IVK93_001560"
     CDS             complement(6427..7029)
                     /locus_tag="IVK93_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6386853.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7214..7915
                     /locus_tag="IVK93_001561"
     CDS             7214..7915
                     /locus_tag="IVK93_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6386854.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7925..10636
                     /locus_tag="IVK93_001562"
     CDS             7925..10636
                     /locus_tag="IVK93_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6386855.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11033..12661
                     /gene="treC"
                     /locus_tag="IVK93_001563"
     CDS             11033..12661
                     /gene="treC"
                     /locus_tag="IVK93_001563"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP6386856.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12721..13902)
                     /locus_tag="IVK93_001564"
     CDS             complement(12721..13902)
                     /locus_tag="IVK93_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6386857.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14154..15680
                     /locus_tag="IVK93_001565"
     CDS             14154..15680
                     /locus_tag="IVK93_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP6386858.1"
                     /translation="MKRLIGILAMLVLAGMLTACGGASGKAEDSKEKLSVMTTFYPMY
                     DFTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQK
                     TLQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVAN
                     IRDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLAL
                     EYGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGV
                     SLEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNG
                     YFEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGY
                     QTDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGA
                     FRYIQFRDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH
                     "
     gene            complement(15749..16477)
                     /locus_tag="IVK93_001566"
     CDS             complement(15749..16477)
                     /locus_tag="IVK93_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6386859.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16490..17770)
                     /locus_tag="IVK93_001567"
     CDS             complement(16490..17770)
                     /locus_tag="IVK93_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6386860.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYAQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17911..18513)
                     /locus_tag="IVK93_001568"
     CDS             complement(17911..18513)
                     /locus_tag="IVK93_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP6386861.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18759..18965
                     /locus_tag="IVK93_001569"
     CDS             18759..18965
                     /locus_tag="IVK93_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386862.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19194..19913
                     /locus_tag="IVK93_001570"
     CDS             19194..19913
                     /locus_tag="IVK93_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6386863.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(19994..20677)
                     /locus_tag="IVK93_001571"
     CDS             complement(19994..20677)
                     /locus_tag="IVK93_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP6386864.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            20909..23095
                     /locus_tag="IVK93_001572"
     CDS             20909..23095
                     /locus_tag="IVK93_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6386865.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7789 a         4197 c         4563 g         6798 t
ORIGIN      
        1 taatttattc tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa
       61 ggttagtaca ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct
      121 tgccgtaaaa ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt
      181 gttgacgcgt tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca
      241 tttgattggc acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt
      301 tgttgctgct tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc
      361 gttcaatttt tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc
      421 ttctttgtag tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt
      481 cagtccattt ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt
      541 acggaattgt gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa
      601 ggtcacgctg cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct
      661 tgataataag ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc
      721 gtagattcct tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg
      781 atcagttgtt tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga
      841 gccgcctgtc agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa
      901 atcaggcgtg aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg
      961 gataaattgt ccgttcccaa tatagatacc tacgtggtgg gtaacaccag aagaaggtcc
     1021 ccagaagacg agatcaccag gttgggcctg acttactgga atctgtgtac ctgcatattg
     1081 ctgttcgcct gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt
     1141 caaaccagaa cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag
     1201 atgcttgaat gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac
     1261 agcatcaatt ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc
     1321 gctttgattt gctgtatttg gacgagatga agcattgttc gttccgccat ttgatgaacc
     1381 attgtttgga tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt
     1441 tgtttgattc gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga
     1501 accattatta ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt
     1561 actagggact tcttcactag gatcagttgg tttctctgtt gaagtatccg gtacaggttc
     1621 aaattctgca gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt
     1681 tacttctttg gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat
     1741 agtgtaagtt accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc
     1801 caaaggttgc tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa
     1861 agaaggagca attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc
     1921 cggatcaatt tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc
     1981 ttctgtaaaa gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt
     2041 gactgtttga tcagctgttt gattatctac ggcaaatacg gtattggtcg acaaaacaga
     2101 cggagcaatc agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa
     2161 ctagtccttt tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg
     2221 aacagataat cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata
     2281 aaacaatttg taaataagca acgagcattc attcgttttt cattgacgtt cattttattg
     2341 catagaccac ctttgtttgg ggataaacaa gtggggataa aaatggttat gcatatgtaa
     2401 attataccaa agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa
     2461 aaagactgaa atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa
     2521 gacattaggt cttcatcaaa cccgctaacg agagtagtaa tcattgctac gactagacta
     2581 acagccagtc ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc
     2641 agactaccaa atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca
     2701 gcagctgcac caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa
     2761 acagttggtt cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa
     2821 cgagtttttg aacgtttact tcgtagaaat acacctaaag ctgcaccagc cattccaaaa
     2881 ttggctgctc ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc
     2941 atgatagggt ttacagccca ttggatacca aatattacca aaatattcca accaccagcg
     3001 atgattgcgc cgctgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa
     3061 ccttcgccaa ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata
     3121 actaatgaaa gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg
     3181 aatttttcaa caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta
     3241 tactgcatca gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa
     3301 agtaaagagg tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca
     3361 ttcactttaa acgttttggc ggcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg
     3421 gcattcgacg cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc
     3481 gtagtcgcta aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc
     3541 ggcgtaaaga tttccgatat caaaccaaat agtttttcga tcggatttag cttttcttta
     3601 ggagctgaat gatcgatttg acgcgttaat tccttgtaag caggagccaa tccctgatcc
     3661 atgattattt ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt
     3721 tttaattttt ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca
     3781 catggaagga cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc
     3841 tgctcggaca gtaccatctg ttcagtattc attgtcgttt ccattagaag accactccac
     3901 tcactaatat aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt
     3961 tattcaactc ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca
     4021 ataatttgat tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa
     4081 tcgattgtac ctcaagttct tctaaaacgt tttctaaaac agataaaaag cttggaagac
     4141 cgtttgagac agccaaatct attttttcac taccaaaggg gacaggcatg cctgcatccc
     4201 cgatacttaa tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga
     4261 ttacttttcc ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt
     4321 gttctaaagc ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag
     4381 aagaggctag atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg
     4441 tgctgcttaa tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt
     4501 taaaagcagg gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta
     4561 gtgtaatgat tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac
     4621 tgttatgatc tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa
     4681 tgatatctgt tttttcaagt agttcagtag ggatcgtttc acgagctggt gcaggattca
     4741 ggatcgttgt ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc
     4801 tactttcaaa ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga
     4861 cttcttgttc atggaaagca ttatttgcac ctgcatggat caagatacta ttttctcctg
     4921 ctgcgtctac cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag
     4981 atgtatcgat gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc
     5041 caacgcctcc aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttgattgg
     5101 cacctttacc tccgccagcg tgaaaaatct catgcgcatg cattgtttcg cctggcttgg
     5161 gcatgttggt taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt
     5221 tcattttaca aacctccaac tatctcattt attttgatgg gaaaaattat tttttaaaac
     5281 gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa agctatttca
     5341 agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct tcccattctt
     5401 tttctgggaa ttggatccga tctaacaata atttggcaga tgtttgtccc aattcgaaga
     5461 tcggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct atattatcat
     5521 agccaataat gctgtagtct tcaggaatgc tttttccaga ttcctctaat ccgcgatata
     5581 atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa tcaagaagtt
     5641 ctgctgtgat ttggtagcca ccttgttttg agaactgtgt aggtaagagg atcagttggt
     5701 cgtgagagat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga gcgtggacat
     5761 tttccggcgg attttcagga tagacaaggg cgatcgtttg atgacctaga ctaagcaagt
     5821 gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca ctaaatcctt
     5881 cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa ttttcgttga
     5941 ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct cgacgtgtga
     6001 gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat aatatcgtga
     6061 cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg aagaatggat
     6121 tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc atttgtctag
     6181 caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc tgaacagttt
     6241 tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg attgaaacgc
     6301 cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc atactcctat
     6361 ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa tcacgtgtgc
     6421 cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc gtactcgtgc
     6481 agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac gatcactgca
     6541 agcatcttca gcaagtacaa catgataccc atgttggaag gcatctaatg cagtagcata
     6601 tacaccgttt gttgtcgcta caccgcttaa aacgatagtt tcgattccac gccggcgaag
     6661 ttgaagatca agagaagtac caaaaaaggc acttggattg tattttgtga tagtcaacat
     6721 attgtttgta tctttcaagg aatccttcaa caacaagtca gtgaattctg ccggagcaga
     6781 caaagcagat attcctccag catctgtctg atgacttaga taagagaaag tagtgatgtc
     6841 tacattcact aaaactttca aggcagcggt acgtttcaat gtttttgcta acaaatcatt
     6901 gtttgtcaga atagattgtg cagtgtgtgg agagagtgta ccgttattta cgatcccttt
     6961 ttgcatgtcg atcgcgacaa aagcagtttt tgataaatca aaaatcatat tttcctttaa
     7021 atcattcatg atgttccttc tttcaaataa caatgttttt tatgtaagta aatcataaat
     7081 cgtttatttg tcaactaaat aaaggattcc tttctttagt ctagagaaat tcagtggtaa
     7141 aataaataag tatgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa
     7201 tggaggaatc agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg
     7261 agaaacgacc attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt
     7321 gattatactt ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat
     7381 ggatacacct gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga
     7441 taaacagctt actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct
     7501 cgttcctaat ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc
     7561 gcttgatcct gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc
     7621 gtcccaatta tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa
     7681 tcccaaactt cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca
     7741 agtattgaaa ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac
     7801 acataattct gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt
     7861 aaggtctgtt gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga
     7921 ttttatgaaa acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg
     7981 acgattcatt gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa
     8041 aactgctgga cctgtcatgc aaaaaacgat ggaccactat gttggtacag caggactttc
     8101 tgatttgcaa atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa
     8161 gatcccagat gcaaaagtag aaacaggaaa acagatctat tattccaata ctggtaaaaa
     8221 cgaagtgatt caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac
     8281 tgatggaaaa ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga
     8341 agggtataaa atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta
     8401 taccatcacg ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc
     8461 gaacgtcgga aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac
     8521 agatatttat tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag
     8581 caaagcttac aaagataaaa tggaacacaa tcaagagaag gcagaaaagt atttacaaga
     8641 tcgccctgaa gaacgcttag aagagctgaa aaaatcggca aacgaagaat tagaaccagc
     8701 acagcaaaaa ataacagaag gcaaagcaca aatagcacag ggaagaacac agttagaaga
     8761 tgctaaaaaa caattagcgc aacagtcagc agctatccag cagcttcctg aagcgcaacg
     8821 aacaatcgct gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca
     8881 attagttgag aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga
     8941 aaaaattgaa aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg
     9001 ttttcaagaa tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt
     9061 tttctttttc tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga
     9121 aaaccgcagg gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa
     9181 aaaatatgca atctatgcaa gtctagcctc agggataggc attgttttag gaacgatatt
     9241 aggaacgaat cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg
     9301 gagtgcgctt attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat
     9361 tgccgcattt ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc
     9421 attgcttcaa ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc
     9481 gctgtggtca cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa
     9541 gtcaagaatg cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg
     9601 cgtgggattg aaagattctc taagttctgt ctcagctaaa caatttggac cgatcattga
     9661 ctatcaagca atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga
     9721 tatgctgcag aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga
     9781 attgagaaaa aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc
     9841 tgatttggat cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa
     9901 aacaggaatc gctattacgc aaaaagcagc agaactattt gacttatcta caggagatca
     9961 tatttcgctt tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa
    10021 aaactatcta ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca
    10081 atcgatgatg gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga
    10141 aatgctttcc gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa
    10201 acaaatcgaa acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt
    10261 cgtattatca ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc
    10321 cgagcgtgta cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac
    10381 gatgtacatt gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg
    10441 tgtaggatat atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt
    10501 tccattagtg atcacatggt ggtcttatgt cttatcagca ggattaacta tcgtatttac
    10561 ggttattgtg atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt
    10621 gaaatcgaat gaataaaaac agaggttgtg aaatcagcgt tttgagctaa taacgagggt
    10681 cagctgctaa ttgaacaccg tgaatcagag gttgtgaaaa agctgtcctg catcgagcaa
    10741 aaggaatact caaataaaat tagctcctca tgttttacat ttgagaagac tgattgccgt
    10801 agataaaagc ttttgagcac catttataga agaaacaaga atattttgct attttaaaca
    10861 agaggataga caaatacatg tctgatctct tgttttttat tgggcaacag acctcttaga
    10921 atgtataaag ataaatttta caaacgcttt ctgttcagtt ataataataa atgataactt
    10981 gtatatataa gttgcggtag gtaataaatc agttgaatgg aggtaaatat ccatgacatt
    11041 taaagacaaa gtcatttacc aaatctatcc caaatcttac aaagacagta atggtgatgg
    11101 aattggagat ttaaaaggaa tcaaagaaaa actcccgtat ctgaatgatc taggcatcga
    11161 catgatttgg ctaaacccta tttatccaag ccctcaaaaa gacaacggtt atgatatctc
    11221 tgattacaca gccatcgatc ctatctttgg tacgatggag gaattcgaac agctaatagc
    11281 cgaagcaaag aaataccgga tagaaatcat gctcgatatg gtgctgaatc acgtttcgac
    11341 agaacatgaa tggttcaaaa aagcactagc aggggaccag cattatcagg acttcttcat
    11401 tttaagagaa gagccaacgg attgggtatc aaaatttggt ggaagtgctt ggtcgccatt
    11461 tggagatacg gggaaatatt atttgcactt atatgatcgg acacaagctg acttgaactg
    11521 gcggaaccaa gcagtaagag aggaactatt caaagttgtt cgtttttgga tggaaaaagg
    11581 ggtaaaagga ttccgtttcg atgttatcaa tgttatcggt aaagacgaac aattgaaaaa
    11641 taacaaagaa aatgatggca aacctgaata tacggacaaa ccaatcaccc atacgtatct
    11701 aaaagaattg aaccaagcaa cgtttgggac ggattccact atcatcacag tcggtgaaat
    11761 gagctctacg acgatcgaaa attgtatctt gtatacagaa cctagtagag aagaattatc
    11821 aatggtcttt aatttccatc atttgaaagt cgattatgaa aatgggaata aatggtctac
    11881 tcctccattt gattttgcag aattaaagcg actatttcat acatggggag aaaagatgag
    11941 tgagggtaac ggctggaatg ctctattttt taataatcat gatcagccac gtgcgctcaa
    12001 tcgatttgta gctatcgatc agtatcgaaa acaaggagca gagatgctag caaccatgat
    12061 ccatttgaac cgtgggacgc cctatattta tatgggagaa gaaatcggca tgatcgaccc
    12121 agactattca tcgatttccg actatattga cgtagaaagt ttgaatgctt ataagttact
    12181 ccttgaacaa ggattcacag aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga
    12241 taactctcga acacccatgc aatggtcaga cacagaacaa gcaggattta ctacagggaa
    12301 accttggtta aaagttggag gcaagctaga agagattaat gtagaaaaag aactatcttc
    12361 tgaagattct attttgtcct attacaagaa attgattcgg ctgagaaaaa catatccaat
    12421 cgttgcacaa ggagattatc atgcttatga agcaaatcat ccggaagttt atggctttct
    12481 tcgccaattt gaaggacagc agctgcttgt attaacaaat ttctatgata aagatacaag
    12541 gatcaccatc ccagaaggat tcacagaagc caatattctt atcgataatt attccgatac
    12601 tgttcttcat caaagtctgg agctaaaacc ataccaggca attgctttgc tgaaaaatta
    12661 aaacaaaaac acaaaacgtc gttcctacga gctattctcg taagatcgac gttttgtgtt
    12721 ttatttaaaa taatcttctt cagaaaatga agagttttca tactctcctt tatcgccata
    12781 aaatttattg tagcgttgtt tgaatttacg gaaaataaaa gtaacgagta atttgactaa
    12841 cgtatagata ggaataccaa aaataacgcc cacaattccc attagatcac ccatgacaag
    12901 taaaacgatt aagatcgtaa tcggatggat ctgtaaacga tcacccatta ctcgaggaat
    12961 caccaagtca ccgtggagaa gttgtacggc aaaccagaca atgacaaatt tgacagccat
    13021 aaaagtagag tcttggaatg caatgatcaa acctggaaag aaggcgataa acggcccaat
    13081 atatggaatg atacataaca acccagcaga aatagctaaa acactagcaa attctaatcc
    13141 gatcagtaag aaacaaaccc agtagactgc tccaaggata aatgaagcaa tgatttgccc
    13201 ctttagaaat gaacccaatt gttgattagc aatctttgtc aaatttttaa aatctgtacg
    13261 aaatgctggt gggacaatcg ctaaaactga ctgataaaat ttttgaggat ctctcaaaag
    13321 aaaaaaagcg acaattggac cagtaaataa agtaataaaa atagctgtaa cagttgaaaa
    13381 aatgttgctg atccgttgtg cgccactttc ccaataattt ccaataaatc cagctatatc
    13441 agacgtcacg tcgtcaatag aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt
    13501 ctctaaaaat tgtcggagac tctcttgaaa atcttgaaac aaatctggca aactactgat
    13561 caatgtcttt gcctgttctt caagaaaagg aacaagccat acgcttccaa gccccaataa
    13621 aaataaaata atcacataaa cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa
    13681 acgtttgatg actggttcca aaagataata caagatcagt gcaaacaata atgggggcaa
    13741 gatagtagcg ataatgattc cgacaggctt gaatataaac gagatctgat ggaaaaaata
    13801 aatgatcagc gcaagtaaga taactattcc taaaacatag taagaggttt ttcctcctaa
    13861 aaagcggatc aaacgattcg ttgtatctgt ttcttttttc actctttttc ctcctttatc
    13921 tataacttca ttatagacaa aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa
    13981 aatatcgtca atcattcata taaactttct catcaattaa aataagaaaa cgcttgacag
    14041 ttcttttaac catatgataa attaaatcct gttgctaaaa cgtaactatt acgttttgta
    14101 aaaggcgaat cggaacattc aaatgaaaga agatgagaag aggagtgtca taaatgaaac
    14161 gattgatagg tatattggcc atgctggttt tagcaggtat gttaactgct tgcggcgggg
    14221 caagtgggaa agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccta
    14281 tgtatgattt tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag
    14341 cagggactga ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata
    14401 cagatatctt tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa
    14461 cattacaaga aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg
    14521 gaagtgaaga aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc
    14581 tagacccaca tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg
    14641 atcagctgat tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct
    14701 ataccgagaa acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac
    14761 aaagatcttt tgtcacgcaa catgctgcat tcaactatct ggcattagaa tatggattga
    14821 atcaagtatc gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg
    14881 aactgaaaca ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca
    14941 aagatagtat tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc
    15001 ctttggaagg attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg
    15061 aagcaaacct cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt
    15121 tgacccccaa aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag
    15181 atcgctctct ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg
    15241 ggacttttga tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag
    15301 aatataaaga atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg
    15361 agaaagagat ggcatttacg tttaaggatg gaacaacgaa acgttcaaat tatcgttatg
    15421 tagggaaaga aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag
    15481 cagaagataa ggaaagtggc gcttttagat atattcaatt ccgtgatcac acgattgcac
    15541 cggctgactc cgaacatttt catttatatg caggaaatga aagtcaagaa gcattgctga
    15601 aagaaatgga gaattggcca acattttatc cagatacaat ggatggacgt gaaattgcac
    15661 aggaaatgct tttgcactga taggcaaaaa agaaccgaag ggtttggatc ggctagtcat
    15721 ttagtcggtc caaactattc ggttcttttt atgaaatcaa tttctttaat ccacggatag
    15781 aggctgggat ggtctcgccg tttgccaacg tgacttctct ggttttttta ttaatcgacc
    15841 gcacattttg tgtattgata acataggatt tatgacaacg gataaaattc cgatgcattt
    15901 tctcgatttc tttgatcttc tcatagaatt gtaattgggc atgagtcaaa tggacttcca
    15961 gtttatgtgg tgctcctagc gaagtagaaa aatattggat gtctttgatc ggtaaaaatt
    16021 tgatctcatc attaaactct attttgaaga tatctttttg gatctgggac atttgaaaat
    16081 cttcgaaaat tttgctcagg atatgctcga cattttcttt taaatcataa agatcttctt
    16141 tgacgatata atcagtcggt tcgatgttgc tttttaagat tttcatcgac atttccgtat
    16201 gggaagtcac atagattatt tttcctaagg gatctaattc acggatcaat ttaccgatcg
    16261 ctacaccatc catttcactg tcttctagtt cgatatctaa aaagtaaata ccaaaagaag
    16321 aagttctgat cttggctgca ttgagaagtt cttccggatc agaggtagaa atatggacat
    16381 aactgtctaa gttttcaatc atgattctat ttttgatcac cgttgataaa acttctaatt
    16441 gttttggatc gtcttcacaa atataaatgg gaatcatatc attactcctt tattttattt
    16501 gtatataaat cgtttgagaa aaacgctggt tttcaattga tgtttcaagg aaaagataag
    16561 gattggtctt tgtcaattca tccaagataa acaagccaag gccttcgttt tttggatttg
    16621 tttttgttga gaaccctttt tgtttcaact ggtttaacgg caatgtattt gttgttgtat
    16681 tgatcacacg aatcatcacg gaatgattct tcttgagtat ggtcaattcg atttttttct
    16741 tctttgcagc actagctcct tcaatcgcat tgtccaagat aataccaagc atacgtacaa
    16801 ggtttaccgt atgtttcgta tccagttcga ttggctcagg aaaatgaagc tggacgacca
    16861 accctttttc ctgtgccatc atgagtttta atgacagtaa actacggatc tcaggaactt
    16921 tcatattatc caacatactt agattggccg gaaaaaggtt catcatctgg cgtgtaggta
    16981 atatcgtttg agcataataa gaccgtaatc cttctaagtc gccttctgaa atatagctgt
    17041 gaagagaaaa aagcagattt tggtaatcat gtttgaattc tctcaagtct tgatagtttt
    17101 tttctagatc tttgatataa tcctgttgca tcctgacttg ttgctctaat aactgatcag
    17161 ctaatttctt attagagatt ttccaataat aaaccaagat gatcaggcca acacacagat
    17221 atagaatgaa aaaactcgta ttcaaagcta agacatcagg tgtttcacca gaaaaacggg
    17281 tataaaaaat aatgatatag tatgtaaata atatcaatgt cccgatcgta ccgatcatac
    17341 ggcggtccac agtgtgaaac caagtctcta acgatttttt aaaacaaaaa gctaataaag
    17401 cagataaaaa agcggataag gcaatgtgaa aatactgcaa tgaatctcct ggttcaataa
    17461 ccgaagattt caaaacaaaa aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg
    17521 tcaataaccc aaaaggaagc gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa
    17581 aagtaagcat cagcattcct atagcaatta gagcagtaaa aatccctaaa atcgaataga
    17641 cccatccaat caaaatacaa gaaatcagaa tgactcccgc aaacagatat ttgtatcgga
    17701 tgtgatggtc aatcaataag atacaaattg tgtaaatcaa atattgcaac gctatggacc
    17761 ataaaaacat aaagtacagc tcccattgat ttttaccaaa ttatagcaca attagcgtat
    17821 tattttttta atttttcagg gattttgaat tcagaactca taccaaagca gcagccagat
    17881 acaaaaaagt cagataagtt agttaattgt ttaatgattg tttttttcat gaaagattcc
    17941 tccaaaattc atcaagtagt ttgtaatagg aagcaacgta gctatttcta aaacaatccc
    18001 aagtagcaaa taggtagaaa agtaagaagc tgagaaaaat aaagtaatga gagaaaaaat
    18061 tagaaacatg ctgatcgcct tctttaataa tgggggacgt ttctcgggtt caatgggatt
    18121 gattgctgta cctcttgatc catagcgaag cagtaacaaa aagcagctga ttaacaaaaa
    18181 gaccatcatt gggaaggaga tagtcaattg cagatgtttc atcaccaagg gaacgcccca
    18241 taaataaagt agtgtccaca gcagacaaac gaattcagag tcggaatgca atccataagc
    18301 atatctgcgg atgcatatat aagaaagaaa ggtgattcct gtttccgccc atgagcctgt
    18361 caccagagaa aagactagaa cgaccgctag ttttccaaga ttattcaata aaacttctaa
    18421 ggcaaatttt acttggacat acaccatatc ttcttccgat ttgtctccgc tgaataaggc
    18481 ttctaataat ttgttcacaa ctttatcttt catctcatac gtacctctac cacaaatttt
    18541 atgctaaaaa aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga catcaaaacc
    18601 gtcatgattt cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt
    18661 ttgatacgaa aagtactttt tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa
    18721 aaattactga ccttataaaa ggtgatggag ggattcttat ggaaaatgaa caattgtttg
    18781 aagtcggcga aacatatgca tttaaatctg atttatttga atcaccaata aaaggaaaaa
    18841 tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca
    18901 atgataccgc tcataatctg cactggcagt tagttgtacg caaatcaagt acaatagaag
    18961 aataaaaaca gataaaatgt tgagagcata aagaggttgt gagtctccgt ttagctctga
    19021 gttacaagga ataatgttaa aaaacagctt ttcatgtttt atgaatattg ccacttgtga
    19081 ccgaggtgtt ggcatacttt tagagaagta gcttttaagg agcagataaa gtcaaggctt
    19141 ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga aggatgccga
    19201 taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacactg actgaaatgc
    19261 ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat gaagtattgc
    19321 cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc tttttgaaca
    19381 aacgcttttt ggcaatcaaa gatgctttca gccatgatga caatgtacta gaccgctcca
    19441 tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt tctgaagtag
    19501 aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa gaaatttcgc
    19561 cacaaaagaa accagatcta ctggtttata ttcgtgtatc ttttgaaact atgttagcga
    19621 gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa ctctattcct
    19681 actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat atatgtccca
    19741 aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt ggagaacaaa
    19801 tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat gcccagtcga
    19861 tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa taatataaaa
    19921 actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc ttagtttttt
    19981 tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat aaatgaaatg
    20041 tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa tgggcgccaa
    20101 acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga acgacactta
    20161 gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa tcatataagt
    20221 tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca cgcccgttga
    20281 ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc gttactaatg
    20341 cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct tcataatata
    20401 gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt agatactttg
    20461 ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca acacttagcc
    20521 attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga gcagaggcaa
    20581 aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat gtcagcaaaa
    20641 gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc ttttatgaat
    20701 atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt ttcagaaaac
    20761 taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat
    20821 agacaacgaa actttaaata accaacttga aataaccaac ttgaaataaa caaattcaaa
    20881 gaagaaagat gggaaggaaa tcaatcagtt gaatgaaaaa gaaattctcc gattacaaaa
    20941 agaattgaaa cgctgggaga acaagtataa aaagcttcaa caagtctttc ggctacaaga
    21001 agaaatcatt gctacctatg aagcggctta tggcgaagca cccggactta ttgaggaaga
    21061 agtacaagta aaagaaagtg aaggaaacag ctctttcttt aaaatgatgg agaaacgttc
    21121 ctataatcgc ttcaatcaag aacaaaaagc agctatcaca tatgatatga agaaacatct
    21181 tcgaatcata gcaggagctg ggagcggcaa aacgcaaaca atctgtgcaa aagctgttta
    21241 tctgatgacg caaaaacaag tagacgaaga acgcatacta atgatcactt ttacaaggaa
    21301 cgctgcaaat gaactaaaaa aacgagtaga taatttcagc cagagaaaaa ccgctgtcca
    21361 tatcggaaca ttccatagta tcttttttag gatatacaat gaaatttgtc gaaaatttcc
    21421 tgaagtagca atgcaaggaa tacaaggaga cttttcaaag gatactgcac aaaaagtgaa
    21481 tgctgttttg caacagctga tacgtaaata taatctttat atatttgatc aatatggaga
    21541 aaaaacgatt gcttctcggt tagactattg gcagaatatg aacctttcag tagaagaaat
    21601 ggttcagtta gttaaagaaa aatatgattc cattgataag aatgcacgac agccgatatc
    21661 agaacgactg tatggattgc tcacggaact ccaagaacaa aaaagaagcc agcaattgct
    21721 agagttcaat gatgtactcc aaaatctgaa aagcgctttg gagaatgagg agatccagca
    21781 ttatatcggc caaaaatatg attatctatt cattgatgaa tttcaagata ctaatccact
    21841 tcaatgggaa atcgtgaaac ggatgacaaa ggaaaacagg atcaagttga ttgttgttgg
    21901 agatgatgat cagagtatct atggattcag aggatcagag ccagcataca tcaagaactt
    21961 tgaaaaagaa tatcctacca aaactctttt cttattaact aattatcgtt ctcgggcagc
    22021 tattgtccaa ggagcaaata gattgatcag ctataataaa aatgatcgga taccaaaggc
    22081 gatgattcca gcacaaaaag aagcaggtgt tatggaagcc tatttgtttt ctgatactaa
    22141 agcagaaagt cagtggctaa tcaaacaaat tcagacattt attcaaaaaa atgggaaata
    22201 taaggaatca atcatattat accgatcagc ttcacaaacg cagcagcttg tccaatctct
    22261 gctgaaaacg aatattcctt ttgtactgga agcagatagt ccatatgaag gtatctttgg
    22321 tatcaagtct ttccaacatt tctatcaaaa aattgttcat tggcaaacag cactgaatct
    22381 gcaaaagaaa aatcaggcct accagcaatt gctccggcag ctgatggcag actgctacct
    22441 gaaaaaaagt gaatgcgatc aatacttttc tcctaaatat caaaatatag ccgttgcaga
    22501 ttatattttg tcgataagcc ccaatttaaa atcaaaatct gcagaaatcc gagagtttga
    22561 ggaagcgttg cgtaaaacag accagcatca agaaatctat tcgcttgttc aaagctatct
    22621 tcatctgcca cgtatagcta aggaaatcga ccagagtgat caagaatgga tacgagaaga
    22681 agtaaagcag catgctactt tcagaagact ccaagcctta tcgcaagaaa cccaagcgac
    22741 agctaaagaa ttaaaaaccc gcttgattgc ttacagacaa ggaaaacttg atgcactatg
    22801 tatccagagc attcacaaaa gtaaaggtct tagttaccga aatgtctttt tgattggttg
    22861 taacgaagga agtttgcctt ataacggagc gacagaaaag aaagtaatcg actttaatga
    22921 aataaaagca gaaccagtga cgacgataga agaagaacga agactatttt atgttgcgat
    22981 gactagagca agaaatcgtc tgtatctatg tgtacctcaa atgaaaaatt caagaaaatt
    23041 gaaagtatcg agatttatca aagaaaccgg gagtcgtgtg aaaaaaggaa agtgatagaa
    23101 gagagggtgt gacagaagtg gtcagtttca agaaagaaga aggaatgtac gaaaattgct
    23161 tctcaaattt ttgtgaaatt tcagcttatt ttcgaagaag ctatttctgg aacaccgttt
    23221 attcggaaaa aggaggtgtg acaagaattt tgtcacacct cccttattta ttttgaaaat
    23281 gagaatacta ttatcaaaat agagaatata tagaataatt ctcattattc aaacgtaaat
    23341 aatcagt
//
LOCUS       DACCCR010000040        22743 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCCR010000040 DACCCR010000000
VERSION     DACCCR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.042"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            493..1200
                     /locus_tag="IVK93_001573"
     CDS             493..1200
                     /locus_tag="IVK93_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6386866.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="IVK93_001574"
     CDS             complement(1259..1903)
                     /locus_tag="IVK93_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP6386867.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="IVK93_001575"
     CDS             2030..2317
                     /locus_tag="IVK93_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386868.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="IVK93_001576"
     CDS             2425..2745
                     /locus_tag="IVK93_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6386869.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="IVK93_001577"
     CDS             2826..4139
                     /locus_tag="IVK93_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6386870.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="IVK93_001578"
     CDS             4136..4855
                     /locus_tag="IVK93_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP6386871.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="IVK93_001579"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="IVK93_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP6386872.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="IVK93_001580"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="IVK93_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP6386873.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="IVK93_001581"
     CDS             join(8416..8478,8480..9517)
                     /gene="prfB"
                     /locus_tag="IVK93_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP6386874.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="IVK93_001582"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="IVK93_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP6386875.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="IVK93_001583"
     CDS             10292..11185
                     /locus_tag="IVK93_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6386876.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="IVK93_001584"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="IVK93_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6386877.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="IVK93_001585"
     CDS             13122..13979
                     /locus_tag="IVK93_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP6386878.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITVVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="IVK93_001586"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="IVK93_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP6386879.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="IVK93_001587"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="IVK93_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP6386880.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="IVK93_001588"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="IVK93_001588"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6386881.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="IVK93_001589"
     CDS             16735..17493
                     /locus_tag="IVK93_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6386882.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="IVK93_001590"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="IVK93_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP6386883.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="IVK93_001591"
     CDS             18406..19437
                     /locus_tag="IVK93_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP6386884.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="IVK93_001592"
     CDS             19434..20201
                     /locus_tag="IVK93_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP6386885.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="IVK93_001593"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="IVK93_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP6386886.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22048..22353
                     /locus_tag="IVK93_001594"
     CDS             22048..22353
                     /locus_tag="IVK93_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP6386887.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            22353..22703
                     /locus_tag="IVK93_001595"
     CDS             22353..22703
                     /locus_tag="IVK93_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP6386888.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         7463 a         3920 c         4908 g         6452 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggttgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gataggctat ctcttgaaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22021 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc caataaaaat
    22081 gttgttttga caggtacttt agcagggatt ggggaatata tcgggattga tcccactgtt
    22141 atccgtgtgc tttatgtatt cctaagctta gttgcttttg gaagtccaat cattttatac
    22201 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22261 aatgcatatt atgaaaaaaa caattatcgt gaacaaacaa aaaaacgcaa agaagcggaa
    22321 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcagcgt attttggtta
    22381 atacgttaac attcatatcg ttggctgttc tttttcctgc gatggtccat gtgagcagta
    22441 tttggacagc cgtccttgcc agcttcgtat tatctgtgtt gaacatgttt gtcaaaccga
    22501 ttctgacgat cctgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
    22561 tcaatgcagc gattcttaaa atgacttcct tttttgtagg agcagaacat tttagttttt
    22621 cttcatttgg tgcagcgtta ttcgtagcta tcttgatgtc acttgtcaat gtaatcgtta
    22681 gtgaacataa cagcagaaag taaaaaaagc tgtgccgaga tctcggcgca gcttttttgt
    22741 tta
//
LOCUS       DACCCR010000041        21962 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCCR010000041 DACCCR010000000
VERSION     DACCCR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.043"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            282..521
                     /locus_tag="IVK93_001596"
     CDS             282..521
                     /locus_tag="IVK93_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386889.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IVK93_001597"
     CDS             complement(883..1143)
                     /locus_tag="IVK93_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386890.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IVK93_001598"
     CDS             complement(1328..1825)
                     /locus_tag="IVK93_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP6386891.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IVK93_001599"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IVK93_001599"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP6386892.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IVK93_001600"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IVK93_001600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP6386893.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="IVK93_001601"
     CDS             complement(4547..5209)
                     /locus_tag="IVK93_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6386894.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="IVK93_001602"
     CDS             complement(5219..6034)
                     /locus_tag="IVK93_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6386895.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="IVK93_001603"
     CDS             complement(6296..6739)
                     /locus_tag="IVK93_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP6386896.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="IVK93_001604"
     CDS             complement(6873..7211)
                     /locus_tag="IVK93_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6386897.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="IVK93_001605"
     CDS             complement(7199..7576)
                     /locus_tag="IVK93_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6386898.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="IVK93_001606"
     CDS             7800..8993
                     /locus_tag="IVK93_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6386899.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="IVK93_001607"
     CDS             complement(9159..9581)
                     /locus_tag="IVK93_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6386900.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9976..10170
                     /locus_tag="IVK93_001608"
     CDS             9976..10170
                     /locus_tag="IVK93_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386901.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10167..10700
                     /locus_tag="IVK93_001609"
     CDS             10167..10700
                     /locus_tag="IVK93_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386902.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQEAI
                     AQQQAEQARQQSAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
     gene            complement(10862..12034)
                     /locus_tag="IVK93_001610"
     CDS             complement(10862..12034)
                     /locus_tag="IVK93_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6386903.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12240..13055)
                     /locus_tag="IVK93_001611"
     CDS             complement(12240..13055)
                     /locus_tag="IVK93_001611"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6386904.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13205..13651)
                     /locus_tag="IVK93_001612"
     CDS             complement(13205..13651)
                     /locus_tag="IVK93_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP6386905.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13648..14652)
                     /locus_tag="IVK93_001613"
     CDS             complement(13648..14652)
                     /locus_tag="IVK93_001613"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6386906.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14692..15021)
                     /locus_tag="IVK93_001614"
     CDS             complement(14692..15021)
                     /locus_tag="IVK93_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP6386907.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15147..17474)
                     /locus_tag="IVK93_001615"
     CDS             complement(15147..17474)
                     /locus_tag="IVK93_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6386908.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18317..18610)
                     /locus_tag="IVK93_001616"
     CDS             complement(18317..18610)
                     /locus_tag="IVK93_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP6386909.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(19013..20193)
                     /locus_tag="IVK93_001617"
                     /pseudo
     CDS             complement(19013..20193)
                     /locus_tag="IVK93_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20297..20611)
                     /locus_tag="IVK93_001618"
     CDS             complement(20297..20611)
                     /locus_tag="IVK93_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6386910.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20718..20933
                     /locus_tag="IVK93_001619"
     CDS             20718..20933
                     /locus_tag="IVK93_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386911.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(21233..21475)
                     /locus_tag="IVK93_001620"
     CDS             complement(21233..21475)
                     /locus_tag="IVK93_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6386912.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21462..21890)
                     /locus_tag="IVK93_001621"
     CDS             complement(21462..21890)
                     /locus_tag="IVK93_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6386913.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6471 a         4323 c         3716 g         7452 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa cacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agcgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agatcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga gaaacaaaaa
    10141 gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga atctgctttg tcacaagctg
    10201 aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc agctatccaa gcgctacctg
    10261 aaaacaaaca agagttgaca agccgtcttg ctgcaattga ttccacaatc aaagcaaaag
    10321 aagcagaaga agaacggaaa aagcaagaag aaattgcacg acaacaagca gaacaagcgc
    10381 aacaagaagc aatcgcacag caacaagcag aacaggcgag gcaagaagca atcgcacagc
    10441 aacaagcaga acaggcgagg caagaagcaa tcgcacagca acaagcagaa caggcgcgac
    10501 agcaatcagc agctgaacag gcctctcagc aggcacaagc cgcagctcct gagcaaaata
    10561 atgaacaaac tgtctatgtc acaccgaccg gttcaaaata ccatacacat aaatgtggaa
    10621 atggtacgta tacccctgcc actttaagcg aagctcaagc tcgtggttta accccttgct
    10681 ccaaatgtta tggaaactaa ttcaaaagtt atactataat aataaaattc ttctgaaaca
    10741 taaaaaaata gaagatggcg ttccagatag taaaccaaaa ataacagttt acacgtccat
    10801 ttcgtcagct tctctctttt ttcatttagt ctgttccctc tgcttttttg gacatcttca
    10861 ttcattcttt ctcaatcaaa tagtacacct ctttttcttt tcgcagttta tatcttcctt
    10921 ttttcaattt cattgaaaac tgtttcgaat gtttatgttt tacatggatt ttaagcagga
    10981 gctcctcatg ttgcaagcga tagttcctgc cttttaaatc agaaaatctc actagtccgt
    11041 attcatcaga agctttcaca aatggaactt tttctctagt aatatagtgt ttgccgttga
    11101 gcgtttttac agaaaagacc attccttcga ttggtcgtcc gtttgtgttc tttacataaa
    11161 aagataaagg atagtttcgc ttagcgatgg ccaaagaatc taaataagcg atgagtagcc
    11221 aaactaagcc gattgatcca agtagtatac cgataaacca aataaaaaat ttccattttt
    11281 tccattgatc aattccttgg atcgcttttt ttgcctcttt tgcttgataa ggaatacgat
    11341 gccccgtcac aagcagtcga tgtgtattga ccatataagg agtacaggta agcaaagtca
    11401 cgagatctct gcctttttgg atacccaaag aatctacttc atcaggtaag accacttgga
    11461 ttttttctac ttgatacgcc aatgtctttc cattgatctg gatgaaaaac tggtctcctt
    11521 ttttcaactt aggcaaatct gtaaataatt ttgcttctgg gagtccacga tgcccagaaa
    11581 gaacggcatg ggtgtttttc ccgccagtcg gataagaact ttcttccaat aatgacgttc
    11641 ctttttgcaa aaatatttcc gttgtctgat cgaatatagg taagctgaca ttgatttttg
    11701 ggatttcgat gactccaagc atatgttcca taaaaaaggc attactaggt aaatctttta
    11761 atgcttttgc atcaactgct tggttgaaac tgcctatccc tggtgatacg ttttgctctg
    11821 ccaactgttg gtttttcttt gtcatttttt cctgctgttt ttttatttcc tctgaattct
    11881 tattcgatgc ttgcttttga taatggatga tcatttgctg ggcaagaaaa tcattcagtg
    11941 aatcttctac aaaaggataa gaaaagatcc cgatcccaac gactaaaatg attgccatga
    12001 agatgtccag tattcttatt ctgcttttat tcatcatgtc cacctatttt ctaatatgta
    12061 tatgaagagg ttgtgaaatc agcgccttga cctgagtatt agcgaagcaa acggcaaaat
    12121 agttcctcct attttgccgt ttgttgagcg aataccaaga gaccgaccac ttgtcacaac
    12181 ctttctcccc atttgaatca ctatcgttta tttatcgtat gatttttgtt tccaatagtt
    12241 taagcttctg tttcttgatg gcgttttttg aaatagatca ctgctcctcc aactaaagca
    12301 gtacctacca agatgaatac gatgatcccc ataccaccag ttgaaggcaa gattccttta
    12361 ggttggttat cgatcgttgt ttcatcagaa gttgttggat catcatctac tgtaaattca
    12421 gtgtacgcag cttcattctt aggtaatgcg tagccttctg gtgcttttgt ttcttgaagg
    12481 cagtatgccc cttgtttcag cccttcgatt gaaaatttcc cttctgaatc agaaatcaat
    12541 ttcacttctt cccagtcaga acctgtgact tcatctgacc aagcatatgg gtattctcct
    12601 gaggcaaggt ttgctatttt tttcattaat ttaacagttg gatcttttgc tcttagaata
    12661 gaaaattctg catcggctaa tttttcttgc gtgttcacat cttgtttgat gaagtggaaa
    12721 tcaccgtagt tttggacatt ttctacagta atcgtgttat ccgtagttgt attttcccga
    12781 ttgactacac cgtttggatt cccaagattg gcttcatctg cgccatggat gataccggtt
    12841 gtttgttgca attttccgtt ttttactata aaagtagttt ctttttgtgg attggttaat
    12901 tgataaccat ctggtgcttt tgtttcaact aaagtataat tgccgtctat taatccattg
    12961 acactgatgg atccttcagt atcagttgtg aatgtcaacg ctgactcagc aggatcatag
    13021 agagcgacac cagtgttttc atcaaaaccg gtcacgaatt gattctctga atttttgatc
    13081 tggaattgcg cccctgctaa cttatctacc cctgaaaagt tcccagcttt ctggattttg
    13141 atattgcttt ctttgatgac atttttagca tataggtgaa tcgtgtctaa aggtttgtca
    13201 gggtctaggg taagcttaac acgatatttg gcgacgtttc gacaccgggc tttggtgttt
    13261 ctacaaagac gtatacagca tctttcccat cagatttacc tggaagagtc aggttagcta
    13321 atccttcacc agaagtcgtt acacttgcga agggtgtagc gtctgctgga actgctgtgt
    13381 ctttacttgg atagtacgta gcagtgatgt catatacgtt gaattcaacg ccgttcaacg
    13441 gttcgctatt cggaaatgtc aattccgttc cgttgtttat ttgattttct ggtgctgaag
    13501 tgaatttcaa tttatgcaaa ttaaccgtaa cattttctgg tgtaggtgat acggctgcag
    13561 aaacctctgc agttcctagg ccgagaacga aaggaagcag catgatagca gttcctaaaa
    13621 acgtccatat ttttgttttg atgtttttca ttttatttcc ctcctattgt ttcctttttg
    13681 ttgtttttta taaaaccaag ctgtccctgt tgaaaggact agcaataacc ctaaaattga
    13741 gaaacctcca agcatctctt cgcttgtttt tggaaagctt cctccatttt tttcatttga
    13801 tggaccattt gtctgcatct gaactggcgt ttttccatta ttcgtcggat ttgcttttgg
    13861 ttcgttgatg atttccaatg gtataggatg aagcggatca taactgcctt tttcaacttc
    13921 aaagagaatg gttgattgac tcaatatata tcctttaggc ggtttaatct ccactagttc
    13981 gtaacttcca aaagacaatc catggacaga aaatgttcca tctttactgg aagtaaaaat
    14041 cgttgctctt gaatcatctt tggctaccca atgatttatc ccgttttctt gcgctaagta
    14101 ttggtcattt tttttgacta gaaattgagc atctgctagt ttttgtttcg tttgccgatc
    14161 cactttgaca aattgtttgc ctcctgtttg gatggcaaga tgtgtttcta ctgtttgatt
    14221 gtcagtcgtc aaacgtacat cgttatcaaa tgtcgttttt tcactgtttt ctttcaatgt
    14281 catttggtaa cggatcgtta atttttttcc agcaaacgaa ctaagatctt ttccagaatc
    14341 tgataagaca aaaccatgtt ttattttttg tgtgtgaaat gtatgaatct tgtctccacc
    14401 aatcaaaaaa tcgatcgttt ctggttctag ccataactga ggatcagctg tatctttgat
    14461 cgtataggtt cctatctcgt tgatgttctc aggaattgtt acggtgatga gataagggac
    14521 tttatcccca tctgcaaaat ctgtctgctt attctctact gtttttgtta atgtgagtgc
    14581 agcaggtatt ttttgttcgc tctttggata caagtggatg gtcgtcaatt tatgattaga
    14641 tgaatctgtc acaggaagca caacgacaaa tggtgctgat ttttctttta cttaatctga
    14701 agtctttatt tccgtaaatc ggtaaacagc gtcacgacct tgctcatctt tgtccgatgc
    14761 actaaaagaa gcaatgccct cttcctcgtc tactgttttt gtcacatttt ctgccagtat
    14821 tttttcacta tcactttttt gtgctagttt tctttgtgcg tcttccacac tacttccttc
    14881 tgaccgcagc ttgtaaaatt catccgagac atcatatata gcgaaagtaa cgccgttcaa
    14941 cccttttact ccttgaaagt tatccgggat atttattccg tcgttttgac tgatacttgg
    15001 catcttatcg ctttcgaaca ttcgattatg caaagtaaat gtaataggtg attctctagc
    15061 tgacacagtc ggagaaccta taaaaaagac cagtaggata aaaaagagaa cagcaaatat
    15121 atccacacgt acattttttg tttttttcat cgctgttcac ctgctttttt cttacgatgg
    15181 ataaaataaa agaagccaga aaaaataaag atcagtccta aaaagacaaa gggcagtaga
    15241 cctatcccac ctgtcgaagg aagcaagaga gtcggattga tcgtcaactt agcggtatct
    15301 gttgcgataa tcgtataatt attgtttaca cttccttcgc ctaaactaat cgttactatt
    15361 tgtgatccaa tatctgtccg gctgttaact gttagggtat aatccaatga ttcgcctaag
    15421 acaacaccgg taacgtggag atttccaacc gaaatcgagt gactttgggc atcataatta
    15481 aaggcagcgt catcgacagt aatcactgcg ttagctttat agatggtata agttcctgat
    15541 gtgttggcta tatcagtaaa tgacaacttg ggatttgctg ctttgacttt gtctattccg
    15601 ctattcgtta gtttaattgt atagctgcca gcattaacca tattttttgc tgattgtgca
    15661 tctgctgtgt aatccatact gctaagtgcc acttgtactg gagtaagcga actagtgaca
    15721 ccctctgcta gcttgacatg gattgacggg acacctgctt ttccatcata tgtcttactg
    15781 ccactgagct ctaccgcacc gctgatttgc ttaatcgtat agacatcaga tgtctgcacg
    15841 atagtaaata aatagtttgt tctggcagct agtttagcta gtccgctttt tgtcaattgc
    15901 caagacgtat aggttccaat ttcagtaggc gcagttactt caactccctt ttcattcatc
    15961 gctttgaaat cagttggatt ccatgtgatt tgatcaatac tgagatcatt gtttaaaagg
    16021 gatgttgatt ctgttttatc attaaaggca tatttaacga tcggtacaac agaaagacca
    16081 tcatagtact tactgccatt tgcattgatt gatactgttg catttgtctt ataatatagg
    16141 atgacttcag aaatctgttt tccatttgcg tcctttacga ttacatttcc atttgtatct
    16201 aaactagcta aaacactcat tgctgattgg ttctttgttt ctatcgctga aacagctgta
    16261 aaggtatatc cgttgatagt aggcgtatta gtcgtcaggt cttgtgcagc acttccgtaa
    16321 gtaagattca ttattttaga aggagcaatc atctgtccaa aatagtcttg atacttaata
    16381 gtgattggtg tttgtttgcc tgtataggtc accgtatcga cgttactttc tagggctgtg
    16441 aaagaaccgt ttgtttgtgc aaaagaatag cccgttactg ctgttggagt gataaccact
    16501 ttgtcaccca catggattcc agataatgta gtcccgtacg ttccgatttt tgctgctcca
    16561 ccattgactg tataagaaaa agttcctgtc cgattcgcat tgacctgaac cgttgctgct
    16621 tgataggttg gaaaatttga aacatttccc ttaccatcaa cgatctgccc atttttgatc
    16681 gagtaccccg tgaatggaat atcatttgct aactgtatac cgattgaaaa agtactcata
    16741 cctcctccgg caggaagccc tccccatgtt gagggtgtaa aagaataact tttatccgca
    16801 ttgggtgtaa ctgaatcgat cgtatttcca gcggtagcca cccccatata gatacttttt
    16861 gggaatgtga tagtagtacc atttactgtg gagccaataa acagattatg gatatctgga
    16921 cggttatcaa taaaagcgat tgctttggtt acgccttgta acgcagtttt atccgcactc
    16981 atcgatccgt taaccgcaac aatgactcca tctttggcat atatcactgt atatccatct
    17041 ggtatggtat gtccgttgaa attcgcagtg gccgcaacag tactaaatgt ctggcttcta
    17101 cctgtttctg aataagcttt ctcccctgct gcttttgctg ctgtgatttc tttttctgtc
    17161 ggatcaatca gttgagtcga ggatgtggtt gaggttgtct cgttgttgct agactgattg
    17221 gtgctttcta tggactgtat ttcagaatag ggtggactat ttgtctcctc tgtcgtttgg
    17281 ttagcaaaat ttgacgtttc tgtataaacg tttacataaa tacttgataa gctccctaag
    17341 aaagtaaaaa tcaagatgat cgaaatcata atgggatgta agtattttct tttagataca
    17401 gctttgagtc tagaaacaaa atgtttttga catatacgtt ggattttatt ttgagttttc
    17461 ttaatcattt tcatccctcc tctcaataaa aaatcatcaa ataatccttt acacgttatt
    17521 ctaaatttaa tttttattat tttattgatg tatagaaagt ttggggcttt ttttctcttt
    17581 ttattctaag gttattttag tcttgtcctt taatacaaag attttatttt taaataaaaa
    17641 atactctttt tttacgatta ttgaaaaaaa aagataaaac ttgattaatt gaagtattgc
    17701 ttaaatttgg aagctatgag ggaagaataa aagagttagg acgggattat ctactatttt
    17761 tctttgtttt aaatatttgt tatctttgaa cataaaagag aaatgtgtcg actgcagtgt
    17821 tgtgtccctc atcacgaata aacggtgttc aactagctga tcttgtattc acttaaaaaa
    17881 tggtaaagta cgagttgcta ttccccattt tcaattttct aacgctcagg acaaaatact
    17941 gttttcacaa cctcattgga ataaaagcat atatattgtt tcaagaacga aatcttgtta
    18001 tctttttaaa gggcatctgt ccaaaatcta attttaggag gtacgttttg gtatttattc
    18061 gtacaaataa aagagaatta tctttattca tgtaacgcat ccaattgatt ttttcatgta
    18121 cgctcctgga acgattttac tgacgattgc ttatttaact gcgaatcgct cattagcttt
    18181 tatcatagct atccatattt tgaataacgt tcttggcttt gtattataga tacagatgac
    18241 aaaaaaagtt caagtatgcg gtgtagaacc aatatcgcat acttgaactt ttttcaatga
    18301 gtataaatta aaacttctaa ttttcaattc ccatcatctg atatgcccga ccgtctgaac
    18361 ttcttttgaa aaagccaaat gtgattagat aacgtctttg ttcgacataa tcgccactag
    18421 aaaatacgtt cagcagtatc gaattaacct ctgtttctgt ataagtcact ccatggtcaa
    18481 agcgtttcaa gacttctttc aataggatgt atttcttttt tgttgaactt ggtacttgtt
    18541 ttacttttcc atcattcatg aatgtgtcta aaactttcat cttttcttgc tccgtataag
    18601 tataaaccat cttctttctc cttttgattc taagaatact ccatttatgt tagaatattc
    18661 tggatttttt attaaagcgg ttactcttaa gaaagatatt atcatagtgc tctttttttt
    18721 actactactt ataggtagat ttattctaat ccattattct cccctaccaa taattaaaga
    18781 aaaaactgga tcagggtgtt caaattacat gctaagaaaa aagaaccctt gaataccaag
    18841 ggttctttct cagtctgata acaatgatta acgacggatt tctttgatac ttgtgaaaag
    18901 tcgttaaagt attgatataa aaggattttt attacctctg actacatatt gactaatttg
    18961 ttaaaaaatc gtttttcaag aatcctgtaa taccagtgct ttcaccgttt ttttaactct
    19021 ctaaagaact aaaagcaatc agaggtttta ccttttcatt tgacgttaag tggcgataaa
    19081 ttttcgaagt tgtttggtca gtatggccta agcgatcttt caagacttcc cagctacagt
    19141 gttccgctac taagcttgcc attgtatgac gagctaaatg agtatgaaat ggcttattaa
    19201 ttcctgagcg attcacgcac cgtttgacgt gatcatttac atactctcga cgataaggaa
    19261 caccacgttt actatagaat caagatacac aaacaaatat gtttcttcta taataaacct
    19321 gtaatgttgc ctcatctcac ccaaagcctt aatcaactgt tgtagctttt ccataactag
    19381 aggagttact agaactcgtc tagcagaact aactgtctta gtagtttcaa ttcgaaaatc
    19441 cttactctta cctttaactg ccgtttgctt atagaacaga atttcatttc ttttgaaatc
    19501 tatatctcca ggctgtagag caagcgcctc gctgactcgg cagccagtgt aaaatagaac
    19561 atggaaaagt acagacatcg gtaattcctc atgaacaatg ccataattca aaaaagttct
    19621 caattcatcg acagtaagaa acttgtcctc aatattattt atggcttctt ttaaatcagt
    19681 taccgttaac tgatattttg gcattctcac agacttcatt ggattttcac ttatcaaatt
    19741 ctttctttga caatatttaa aaatcatatt tagaacttga ataagtgatt gtaaactatt
    19801 tttggcatag ccaacatttg ttccattaac gccacattcc tttaaggtaa aaagcaattc
    19861 ttgaaggcac tctggtgtca aatcagacaa ctttttatca ccaatccatc gaacaataat
    19921 ttttaattgt tcctctcgct tatcataggt tatttccttt tgttcattta acgaacggta
    19981 atattggaac cattgctcac ctacaacact aaacttgtca ttgcttgcaa caattttttt
    20041 cttaagcctt tgttgtttct ccatttccag ataagcgagt tgcgcctcac gttttgaatt
    20101 aaagccacgt cttgttgttc gatatgcttg aaagtaccat gtgatcctac cagtagaact
    20161 gatttgcttc ttgaatcgat ctgttttttc catttattga ttcctcctat tttgaaggaa
    20221 cccaacaagc agaaaccctt tggaaatctt tctatctatg tcatcattgt accgcatctt
    20281 agaaagaaaa tgtaacttat aaagatcgaa gtagtcgcaa tttttctcga tagtcattcg
    20341 aatcactctt tctcatccat tctaaaacat catcacgaaa atagtgtcct cgaatcccgt
    20401 accaacaggg aggaaactca ccaagtttta ctaaacggtt cagtgtacta tcacttatct
    20461 gaaatagtcg ctttaaatca gtatttgtta ataccagagg taaatgactg actgcaagca
    20521 atttttccgt gtaatcttcc agtttttttt gtaaaattga atagaactct atttccagag
    20581 aattaacatt tgttttaact tccaactcca tcacccttct ctctcaaaat caaaacgacc
    20641 accacggtac cccttaaaat tgtaaaaaaa cactccagta ctccatcaaa acggactaga
    20701 aaagaggaat ttttaatatg aaacaaggaa ttgcagacat aaaaataatt aaagaaattc
    20761 ttgaaaaatc tacggctaat gcaatagcat tcggaacagg aatcaacttg agtacagtaa
    20821 aaaaacttaa aagtggagag agagctgaag aaaaattaaa tcttgccgat gctattaaaa
    20881 tcacagaatt tggcatgaaa aatatgccaa ccaaaatcga aatatggaaa tagtagtttt
    20941 tctgatctat cagctgtttt gccaaagcac ataggaatct cacctccccc cagtaatggc
    21001 gggccgattt ggttacggaa gtatcattat cgtagtcgcg attctcatat taaaatttgt
    21061 cgcttaaaag ctaaccttgt catggcgtaa ctagagaatc aaaatctaca tgtactgata
    21121 gatttgctat tcatttgtca aagagcgcta ggctattttc agcctatgga aatcctttat
    21181 tttgacccga taaaaaactt ccatagaatg aaaaggaaag ggaaaaaata ctttaacgag
    21241 ttgtatcaaa ttcaagtatc ttctcaatca attttgtttc cagttgtcta cgcatatatt
    21301 catctacata cataaccatt cctttttctt cttcataaac tggtttcatg gctaaccgtg
    21361 aaatataatt ttgatatttt gacagaacga tggacatggc aattgaatca ccagaagctg
    21421 cggctttgat tgtcgttagt ggtatgaatg gtatatatcg tttattttgc ttcatatttg
    21481 acacccaaat actttcctag ctcttccaaa gccatttgtc gttggtacca aacactgccg
    21541 agacttttcc caataatatc gccaatttca cgatcattaa gaccgatgaa gaaggacata
    21601 aaaatgattt cttgtctttg actaggtaat gctgcaatcg catttgccaa ctcctcattg
    21661 gaaataaaga ttttcatacc aaggacaata aaagtaccac tactattcaa atttggttcc
    21721 aaataataga actctgtacc taaccctttt tgttccaata tttccattga tatttgccgt
    21781 tggcgaaatc tagcgtactt cttttgaata tttaacgcct cattgcagac tactttctta
    21841 caaaaactgt caaacgtatg ttgaatgtgg gtttcctctg agtcgcccat aatattctcc
    21901 ccctcaaaaa attcattcaa ctcgttttac ttctatccct ttccattatt actacaatct
    21961 ag
//
LOCUS       DACCCR010000042        21911 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCCR010000042 DACCCR010000000
VERSION     DACCCR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.044"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(308..508)
                     /locus_tag="IVK93_001622"
     CDS             complement(308..508)
                     /locus_tag="IVK93_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6386914.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(813..2045)
                     /locus_tag="IVK93_001623"
     CDS             complement(813..2045)
                     /locus_tag="IVK93_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP6386915.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2299..2868
                     /locus_tag="IVK93_001624"
     CDS             2299..2868
                     /locus_tag="IVK93_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6386916.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3046..3486)
                     /locus_tag="IVK93_001625"
     CDS             complement(3046..3486)
                     /locus_tag="IVK93_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP6386917.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3644..4408
                     /gene="map"
                     /locus_tag="IVK93_001626"
     CDS             3644..4408
                     /gene="map"
                     /locus_tag="IVK93_001626"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP6386918.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4440..5363
                     /locus_tag="IVK93_001627"
     CDS             4440..5363
                     /locus_tag="IVK93_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP6386919.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5439..6578
                     /locus_tag="IVK93_001628"
     CDS             5439..6578
                     /locus_tag="IVK93_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP6386920.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6571..7371
                     /locus_tag="IVK93_001629"
     CDS             6571..7371
                     /locus_tag="IVK93_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6386921.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7371..8198
                     /locus_tag="IVK93_001630"
     CDS             7371..8198
                     /locus_tag="IVK93_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6386922.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8176..8910
                     /locus_tag="IVK93_001631"
     CDS             8176..8910
                     /locus_tag="IVK93_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6386923.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9010..9876
                     /gene="rfbA"
                     /locus_tag="IVK93_001632"
     CDS             9010..9876
                     /gene="rfbA"
                     /locus_tag="IVK93_001632"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP6386924.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKISVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9890..10462
                     /gene="rfbC"
                     /locus_tag="IVK93_001633"
     CDS             9890..10462
                     /gene="rfbC"
                     /locus_tag="IVK93_001633"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP6386925.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10484..11512
                     /gene="rfbB"
                     /locus_tag="IVK93_001634"
     CDS             10484..11512
                     /gene="rfbB"
                     /locus_tag="IVK93_001634"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP6386926.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11610..12461
                     /gene="rfbD"
                     /locus_tag="IVK93_001635"
     CDS             11610..12461
                     /gene="rfbD"
                     /locus_tag="IVK93_001635"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP6386927.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12496..14529
                     /locus_tag="IVK93_001636"
     CDS             12496..14529
                     /locus_tag="IVK93_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386928.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14573..15853
                     /locus_tag="IVK93_001637"
     CDS             14573..15853
                     /locus_tag="IVK93_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP6386929.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16063..16869
                     /locus_tag="IVK93_001638"
     CDS             16063..16869
                     /locus_tag="IVK93_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6386930.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16881..18101
                     /locus_tag="IVK93_001639"
     CDS             16881..18101
                     /locus_tag="IVK93_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6386931.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18091..19677
                     /locus_tag="IVK93_001640"
     CDS             18091..19677
                     /locus_tag="IVK93_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6386932.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19716..21854
                     /locus_tag="IVK93_001641"
     CDS             19716..21854
                     /locus_tag="IVK93_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6386933.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6914 a         3709 c         4551 g         6737 t
ORIGIN      
        1 ataaatctat tcgaagggtc aaatttaaac cataattgtg tttttccttc catcagcata
       61 aaagttcacc tcaaaaagag tatacgaacg aatgttcttt ttgtgaaggc ggaatttaat
      121 atactgaata atagaaaaca agtacttgtg ccaaattatg tgccaaaaaa attcgaattc
      181 aacaaaatgt aaacaaatag aaaaccttct atattaagat ttcctgcaat aacaaacaca
      241 acagaagacg tgtaatagtt agtcaggaac gtacaaataa cccctgtatc ctttgcggta
      301 cagggggtta ttttgtataa gtagacattg tagtttctaa actgctatgt cctaaacgtt
      361 tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgc gtatgtctta
      421 gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa gaaaaactta
      481 ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa cgatttcgat
      541 tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag cggtataact
      601 tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt atttttagta
      661 atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa aaaaacggtg
      721 gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat tctatgcttt
      781 atattatttt ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt tcggaaaatc
      841 ggtactgtag agccatcttc acggattccg tcgatatcca tcttgtcaga tcctaccatg
      901 aaatctacgt gggtttgact tctattcaat ccagcttctg ctaattcttc atcggacatt
      961 tctgtaccgc ctttcacact gaaggcataa gcagaaccta gtgctaagtg attcgatgcg
     1021 ttttcatcaa ataatgtatt gaaaaagatg atgccagatt gagaaatagg tgaagggtca
     1081 ggtacaagtg cgacttctcc taaacgacgc gcaccttcat ctgtatcaag cagttttgct
     1141 aagacatctt ctccttgttc ggcagaaaaa tcaacaactt ttccatcttt aaaagtaaat
     1201 ttcatacccg agatgatggt gccagcataa ctcagtggct ttgtacttga aatgtaacca
     1261 tctactcgat gactgtcagg tgctgtgaac acttcttccg tcggcatatt tgccatgaat
     1321 ttttctccac gagcattata gcttccagcg ccttcccaaa gatggttttt aggcaatccg
     1381 ataatgatat ctgtaccggg agcagtatag tgaagggcag aaaattgttc tcgattcaat
     1441 tcttctgctt tttttgctaa tttttcatca tgcttcttcc atgctaagac agggtcttct
     1501 tcatagacac gagtcgtttt gaaaatctga tcccataatg cctcaacttg tttttcttct
     1561 ggcagttctg ggaagacttt tgctgcccat tgtttaccag ccgcagccac gacagtccaa
     1621 ctgactttat ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc attcgctgat
     1681 tgataagacg ctactcgatg gctatctaca ccagcaaatg catcgggatc ggcagaaaca
     1741 acgctgatcc tgcttgctcc tttttcaagc cattcatccg cttgatcgat tttagattgt
     1801 ggtacattct caatacgatc cgttgcagca tgtaaaagaa attctctttg gatctggtcg
     1861 tcagtccatt gaacgataac ctcagcggct cctagtttat aagcttcttg tgtgatcaat
     1921 cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc tttttctgtg
     1981 gctacgccag tttctgcaat taagcgtgca tattttttta gaagttcatt aaaatcagat
     2041 aacataataa aaattcctcc tcaccatttt ttctggtaac tattagcatg ataccattta
     2101 attatgattc aaacaatgag gctttaaata aaaaaaggag gttatttttt taaggttaag
     2161 tttcatgata tatgttacta cttgctattt cagcttgtga agaaaatcaa aaactaattt
     2221 tgcatatcaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt tataaaaaaa
     2281 ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca gattttggca
     2341 gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag aaacatcgcg
     2401 aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat ggatgacttg
     2461 agacaagctc tttttgagca gatctatgat catttacaat atgaagtgtt cactgttgag
     2521 catacaggga atacgattat cgatcttgct tttaattata tccattttgc aagaagagag
     2581 aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga gatgcagaat
     2641 ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt gaatctttca
     2701 gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg gatcgcagct
     2761 ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat gatccaagac
     2821 agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat atactcaaaa
     2881 gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt cgtttttctt
     2941 acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga ataaaagagc
     3001 catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa tttttgcgac
     3061 tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat tcagatccac
     3121 tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat cgaaatcatc
     3181 caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac cgtaaatttt
     3241 tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat caggcagatc
     3301 gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga attcatctgc
     3361 atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa atgcttctgc
     3421 aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga tttttgctaa
     3481 agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata ccaaaacatc
     3541 attgaaaaga aaataaaacc taaagacacc tggggaatgt catttggcta aatgtatgcc
     3601 gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta ctttaaaatc
     3661 agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg tacataaaaa
     3721 tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat ttgtcagaaa
     3781 ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt atgaatatgc
     3841 gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa agcctttgaa
     3901 aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca tttctgattc
     3961 ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga tggaagtaac
     4021 gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga ttggtgacat
     4081 cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc gtgattttat
     4141 tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt acggagaagc
     4201 tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta tggttaatac
     4261 cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa gagacggcgg
     4321 actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa gaattttgac
     4381 atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg gagaggtaca
     4441 tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt gagacgactc
     4501 aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct tattatttac
     4561 tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt ctccacatcg
     4621 atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt taccagttta
     4681 tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta tccgtttctg
     4741 cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt gcgatgaata
     4801 aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc tcactaattg
     4861 tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg ttgggacagc
     4921 tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc gatcagttcc
     4981 aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc ttgatttatt
     5041 taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg atcttcgcta
     5101 ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat tttgcaacga
     5161 ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg cttaattttg
     5221 ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa tacgtgacgg
     5281 gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa ttgaaaacta
     5341 aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat atcagtaatt
     5401 tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct tttagtatgg
     5461 ttataagatt ttttctgaca attttattat cactgatatt aacacctgta ttcaaaatca
     5521 tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat aaagttccaa
     5581 tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact ttattcttat
     5641 tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct ttagtaatca
     5701 tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa agtataggga
     5761 tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat tccttcacgc
     5821 ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg attttttgga
     5881 tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg gcggcaggga
     5941 tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc catgcttcta
     6001 cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt ttttttcctt
     6061 acaattttta tccggcaaag atttatttag gcgatacagg agctctattc ctagggttta
     6121 tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca tcgatttctt
     6181 tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt aggagaaaag
     6241 caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga ctaatcagtt
     6301 taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg atgttttctt
     6361 tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg attattgcca
     6421 tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag aaacatcagt
     6481 ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa gtaaaacaga
     6541 aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca gccgatcctt
     6601 attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa ttttatttca
     6661 actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc acttcccgag
     6721 tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta ttttcatgat
     6781 gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga ttctcgctat
     6841 ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc attggtcttg
     6901 ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa tgaagatgga
     6961 acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt gcgctttgtg
     7021 cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga atgccgagga
     7081 ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat ggtagctgat
     7141 attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta ctttgaagac
     7201 aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac acccttttat
     7261 gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt tcgcgtgttt
     7321 ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg atgaaaccag
     7381 aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca gcttatggac
     7441 cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat aatagtcctc
     7501 aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat gatccgcaaa
     7561 atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa ggaacacgtt
     7621 gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa ctattgcgta
     7681 aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct gggagccgac
     7741 aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca gtagaagttg
     7801 aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg tctgtcaaat
     7861 ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg gatcattggc
     7921 tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta cgaatggaac
     7981 atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt tctattttac
     8041 aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt caccgacgac
     8101 aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat atctggcgga
     8161 tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc ggtttgtata
     8221 gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt atctcagctt
     8281 tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac atgggatata
     8341 ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc tcgtcaagga
     8401 ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat ctttttggca
     8461 gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta ttttgagcag
     8521 aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga gctgcaagag
     8581 atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa taatgtgata
     8641 agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc cgatattttg
     8701 ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg aggaaagaag
     8761 actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa cgcgagtgag
     8821 attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct gattacggcg
     8881 ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt aatatttcca
     8941 tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca aaaaggagag
     9001 cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct gtacccttta
     9061 acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat ttattatcca
     9121 atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac gccacaagat
     9181 acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca gatcgaatat
     9241 gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga agaatttatt
     9301 ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg aggtctgtct
     9361 aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg ctatcatgtc
     9421 aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc tttgtcgatt
     9481 gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta tttttatgac
     9541 aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga actagagatt
     9601 acggatgtca ataaagcata tttggataaa ggaaaaatat ccgtagaact tatgggacgc
     9661 ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc tacctttatc
     9721 gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat tgcttaccgt
     9781 atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa aaaaaatcaa
     9841 tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga tgaaagtaac
     9901 agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg atcatcgcgg
     9961 tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga aatatgactt
    10021 tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc ttcatttcca
    10081 aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag ttttagatgt
    10141 agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat ttatcttatc
    10201 tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat tcgttacttt
    10261 gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag aagcagatgg
    10321 gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc catcaagagc
    10381 gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag cagaaaatcc
    10441 atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa acatcattgt
    10501 taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa acaatcatcc
    10561 agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag agaacttggc
    10621 cggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg cagaattagt
    10681 ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat cccataacga
    10741 taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta cgtatacact
    10801 tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg acgaggttta
    10861 cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag gagaaaaatt
    10921 cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag ctggttcaga
    10981 tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt caaactgttc
    11041 aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag taacgagtat
    11101 cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg actggatcca
    11161 tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa ttggcgaaac
    11221 ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat tgatcttaga
    11281 aatgatgggc caaccaaaag acgcatacga ccatgtaaac gaccgcgcag ggcacgattt
    11341 gcgttatgca atcgattcga caaaattgcg agaagagcta ggttggcagc ctgagtttac
    11401 gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg aagactggtg
    11461 gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat aatacagagg
    11521 aaatttcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg tctctttttt
    11581 aaaataaaaa tcaaggagcg gataaccata tgtttttatt aactggagga aatggacaat
    11641 taggtacaga attgcgccat ttattagatg aaaaaaatct gccatatgtt tcaacagatg
    11701 caaaagactt ggatatcaca gatgctgaga aaacaatggc ttatattaca gaattaaaac
    11761 ccgatgttat tttccattgc gcagcttaca cagcagtaga taaagcagaa gaagaagcaa
    11821 aagaattaga tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt gcagcaaaag
    11881 ctgtgaatgc acaattcgtc tatatcagta cagactatgt tttcgatgga aaaaacaaaa
    11941 atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt cgtacgaaat
    12001 tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata cgaacttctt
    12061 gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta gcggaaaccc
    12121 gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca cgtactttgg
    12181 cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat catttgtcga
    12241 acgaaaatag ctgttcttgg tatgagttcg caaaagaaat attgaaagat acagatgtag
    12301 aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg cagtattcag
    12361 tcatgagcct gaaaaagaca gaagcgttag gttttgtcat accaacttgg caagaagcac
    12421 ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt tatcaaatag
    12481 aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa
    12541 gcgaatcagg cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt
    12601 ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt
    12661 tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag
    12721 aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct
    12781 gaagaggact tttcgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt
    12841 gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc
    12901 tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg
    12961 gtcttgcttg gattcgaagt actgatgata ttaacgaaga ggaaaaaagc tctatcgcta
    13021 atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg
    13081 ggcgatttga ttttctttac tttgggactg atgattgctt tttatcatta tttctacaat
    13141 catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg
    13201 tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat
    13261 tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg
    13321 ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca
    13381 aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga
    13441 ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa
    13501 gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta
    13561 ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga
    13621 cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa
    13681 tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt
    13741 agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt
    13801 attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg
    13861 attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg
    13921 ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct
    13981 tttgtcgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga
    14041 gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt
    14101 aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc
    14161 ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa
    14221 gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat
    14281 acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca
    14341 gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta
    14401 tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa
    14461 gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc
    14521 gtacagtaat aggctagctg agaataatcc tgaaatatta gtagaaagga gagtgaagat
    14581 aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt
    14641 gctgacgcta gtcttttttg tttggacagc aggggtaggt tttccagcaa ctttgtttat
    14701 atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt
    14761 ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt
    14821 cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt
    14881 gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat
    14941 ttatctgatt gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat
    15001 ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat
    15061 caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc
    15121 cagttctttt gaacgatggt atgttcgaat catcggtggg atagcagtcg ttgcagcctt
    15181 tttaggtatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat
    15241 attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcctacac
    15301 ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa
    15361 tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc
    15421 tgaacaagtt ttactaggaa tgaaaacatt tatcttccag cgaggaaacc aatttttagg
    15481 aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc
    15541 tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg
    15601 gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac
    15661 attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg
    15721 atatgatgaa ccagaagtta cggaggaatt ctatgttatg gaagaggcat atgacgatat
    15781 gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca
    15841 acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt
    15901 tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta
    15961 tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg
    16021 cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat
    16081 ttatttatga aggacattta tcagaacaga aaattactgc tacagttttc gatcaatgac
    16141 ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta
    16201 tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat
    16261 gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca
    16321 gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc
    16381 gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccattta
    16441 ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg
    16501 tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg
    16561 ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg
    16621 cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt
    16681 gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt
    16741 ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg
    16801 ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat
    16861 gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat
    16921 cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga
    16981 aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg
    17041 agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta
    17101 cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc
    17161 tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg
    17221 gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat
    17281 ttgctgacat cggcgaccat atttatcaac ctgtaaaaac ctattcaagt gggatgttcg
    17341 ttcgtctagc ttttgctgta gcgatcaatg tgaatcctga tattttgatt gtggatgaag
    17401 cattggcagt aggagatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca
    17461 agaactcggg aaagacgatt ttattcgttt ctcatgatat tagttcgatt cgccgttttt
    17521 gtgatcgttc ctattggttg aaaaatggaa aagttgtcga atatggtgat acgatggaag
    17581 taacgacgaa ttacgaaaac tttttgaaga aaaaatcagt taaaacagta gatcgaaata
    17641 aaaaccaagt agaacgcttc gcagctccag atatcgtaga cgtgacgaaa gccgaattgt
    17701 tggataaaga taaaaatcca gtggatatgc tggaacaagg agaaaaacta tacgttaaag
    17761 taacctatga agtaaaagac gatacaatca aaaatccagt tttaggaatt gctctgcgta
    17821 cagtcgacaa tttttatgtg tgcggactca atactctact agatggtatc aagatccctt
    17881 ggaaaaaagg tgagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg
    17941 aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatacaaga
    18001 caaaatacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag
    18061 accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat
    18121 gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag
    18181 tttggtcctg gaaacggccg attaacgaaa catttgatcg gagcaaaaca atgtgaagtg
    18241 agcattgtcg agctagataa ggaattgttc gattttgtca gcgagtttgc ccaagatgga
    18301 ttttatggag atatcgaaag ttttgaatgg gcaaactatt atgctggaca aacatttgat
    18361 tacgttttgt ttgccgatgt tttagaacat ttggtagatc ctggaaaaac gttgaagaaa
    18421 gttagagaat ttttgaatga agaaggagaa attctaatta cattccctaa ccttgcgcat
    18481 aattctgtca tgattgattt gttcaataat caattgccat gggcttctta tggtttgcta
    18541 gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagca
    18601 ggattataca tcaatattga agattatttg tacttagctg taggtgatac agaattaaaa
    18661 tcaacttatg aagaattgcc ggaagctgta cgttatgatt tcaaaatgcg accatttggc
    18721 gaagtctacc aatacttctt ctcattgatg aaacatccag tagcacaaag ttccattgcg
    18781 cagccgcaaa attctaatta tgtgaaagta ttagaagtaa tccagcaaac gaaacaagat
    18841 gaaataattc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
    18901 catgtatcag aattagcaga acgaatggtg tttcgcttcg cgcagcaacc tagctttatc
    18961 gaattcagcg cagaagctgc tggagaaaaa ctcacattta tcgattcaaa tgctgtagtc
    19021 aaaacagtaa atgattgtta tttgtttgat ggtaaagatt taccagagtt ctttttaacg
    19081 gatattgcag ggaaagaagt gactatccat tgtcactatc gttttatcgg agaattgaca
    19141 ccaacaatga aagaattgct ggaaacaatc cgtccaatgg ctgaagttac aaagcagctt
    19201 tctgagaaaa atgatgaatt agaaagagaa aatcattatc ttattgagaa taatacaaga
    19261 ttagatcaga cgttgaaaac aaccataaat cgctattgca cgttgttgga aagtgatgaa
    19321 tggacaatca aacatagatt agggcgacgt aagaaagaaa catcgaaaaa aatccaagaa
    19381 aaagagcttt ctctatgtat tgacgaaaaa atctgggatg cagaaacaaa gatattgaaa
    19441 atcatcgggt ggggaatcgc taattcggat cgccaaccat tgtcctataa gttatcagcc
    19501 gaccaatcac cattttttga agcaattcct gttttacgag aagaagtcaa tcaagcaaaa
    19561 cagctagctg aaggagcaaa agcaggattt gaactgcgga tcctttgcga gaaggaacgt
    19621 tcattcttag ttgaagcagt ggctcaaaat ggtcaatcat ggatcattga aatgtaaata
    19681 atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat
    19741 ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa
    19801 ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg
    19861 aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc
    19921 aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagcaatc ttagacttcc
    19981 aaacagcaag acatcaaaat ggcatagctg tgaaattaaa cggtaaatat ccttatgatg
    20041 acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg
    20101 caaggactca tggcgtaaaa aaagcaatcc gccgggtcaa actggaattg aaaccaggat
    20161 cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc tgatctgaaa gagcaaagga
    20221 aagaagtaca aggatttgcc taccgcccat tgatttccat acttatgcct gtctataacg
    20281 ttgaaatcaa atggctcgaa aaatgcattg attcggtact tgatcaaacg tatgatcact
    20341 gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctagaat
    20401 cctatcaagc aaaagatgat agaattaaag tcgttttccg acaagaaaat ggacacatca
    20461 gtctggcaac taattctgca ttggaaatgg cagaaggaga gtttatagcg ctcttagata
    20521 atgatgacga attacctcca tttgccttat atgaagtggc aaaagtgcta aacgtacatc
    20581 cagaactgga tttgatctac agtgacgaag ataaaatcga tgcagatggg aatcgctttg
    20641 acccgcactt caaagcagac tggtctcccg atacattgat gggaaataat tatatctctc
    20701 acttaggcgt atatcggaca agcattgtta aggaacttgg cggtttccgt aaaggatatg
    20761 aaggctctca agattatgat ttggttcttc gtgtgacaga acaaattcct gcagaacaca
    20821 tttaccacat tgaccgtgtc ttgtatcatt ggcggacgat cccgggatca actgcaagca
    20881 atggagaagc taaaagctac atctatgatt ctggagtgaa agcattgaca gatgctctat
    20941 ctagaagaaa tatcaaggga agcgtacatc ctggcagaat ttctggtttt tatgaaatca
    21001 cttatgatgt tttgcaagaa gacttggtca gtgtgatcat tccaactaag aatggctatg
    21061 aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttatcca aattatgaaa
    21121 tcattattgc agataatgga agtacagatc ctaaaatgca agaattattt gcagaataca
    21181 agcatcagct gaaagaccga tttatcgtcg aattaattga cattccattc aattattcac
    21241 gtatcaacaa tctggcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg
    21301 atacagaagt gattgaacct gactggatga cagcgatggt ttcttatgca cagtttgatc
    21361 gaatcggctg tgtaggagca aaattatttt atccggatga tacaacgcag catgcaggtg
    21421 tattattagg gatcggtggc gttgcaggac atgcattgaa taactatgat cgaacacatt
    21481 gtggttactt tggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta
    21541 tgatggtcaa ggcagctgac ttcaatgctg tcaatggatt tgatgaaata ttagaagtag
    21601 cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg
    21661 cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa gatacaccag
    21721 aaaaacaaag acgttttgct ggggaaatca aaaaaatgca agataagtgg ccttcttata
    21781 ttgctcatga cccattttat aacgataatc taactaaaga aggaatcggc gatttttcgt
    21841 tgagacctga ttgattaaaa gaactgtatt ttcattttta ttaatgaaaa tacagttctt
    21901 ttaatttaat t
//
LOCUS       DACCCR010000043        21500 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCCR010000043 DACCCR010000000
VERSION     DACCCR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.045"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            2..400
                     /locus_tag="IVK93_001642"
     CDS             2..400
                     /locus_tag="IVK93_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6386934.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IVK93_001643"
     CDS             384..1079
                     /locus_tag="IVK93_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP6386935.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1663..2550)
                     /locus_tag="IVK93_001644"
     CDS             complement(1663..2550)
                     /locus_tag="IVK93_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP6386936.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2735..3574
                     /locus_tag="IVK93_001645"
     CDS             2735..3574
                     /locus_tag="IVK93_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6386937.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3770..4744
                     /gene="sgrA"
                     /locus_tag="IVK93_001646"
     CDS             3770..4744
                     /gene="sgrA"
                     /locus_tag="IVK93_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP6386938.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5042..5398
                     /locus_tag="IVK93_001647"
     CDS             5042..5398
                     /locus_tag="IVK93_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386939.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5401..6558
                     /locus_tag="IVK93_001648"
     CDS             5401..6558
                     /locus_tag="IVK93_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386940.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6584..7024)
                     /locus_tag="IVK93_001649"
     CDS             complement(6584..7024)
                     /locus_tag="IVK93_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6386941.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7184..7690
                     /locus_tag="IVK93_001650"
     CDS             7184..7690
                     /locus_tag="IVK93_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6386942.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7858..9771
                     /locus_tag="IVK93_001651"
     CDS             7858..9771
                     /locus_tag="IVK93_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6386943.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9776..10777
                     /locus_tag="IVK93_001652"
     CDS             9776..10777
                     /locus_tag="IVK93_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6386944.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10979..11185)
                     /locus_tag="IVK93_001653"
     CDS             complement(10979..11185)
                     /locus_tag="IVK93_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP6386945.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11831..12679)
                     /locus_tag="IVK93_001654"
     CDS             complement(11831..12679)
                     /locus_tag="IVK93_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6386946.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12843..13745
                     /gene="gnd"
                     /locus_tag="IVK93_001655"
     CDS             12843..13745
                     /gene="gnd"
                     /locus_tag="IVK93_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP6386947.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13747..14748
                     /locus_tag="IVK93_001656"
     CDS             13747..14748
                     /locus_tag="IVK93_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6386948.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14745..15029
                     /locus_tag="IVK93_001657"
     CDS             14745..15029
                     /locus_tag="IVK93_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6386949.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15062..15859
                     /locus_tag="IVK93_001658"
     CDS             15062..15859
                     /locus_tag="IVK93_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6386950.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15873..16706
                     /locus_tag="IVK93_001659"
     CDS             15873..16706
                     /locus_tag="IVK93_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6386951.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16724..17581
                     /locus_tag="IVK93_001660"
     CDS             16724..17581
                     /locus_tag="IVK93_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6386952.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17603..18112
                     /locus_tag="IVK93_001661"
     CDS             17603..18112
                     /locus_tag="IVK93_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6386953.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18149..18550
                     /locus_tag="IVK93_001662"
     CDS             18149..18550
                     /locus_tag="IVK93_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386954.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18662..19015
                     /locus_tag="IVK93_001663"
     CDS             18662..19015
                     /locus_tag="IVK93_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386955.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19049..19492
                     /locus_tag="IVK93_001664"
     CDS             19049..19492
                     /locus_tag="IVK93_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6386956.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19509..20594
                     /gene="uxuA"
                     /locus_tag="IVK93_001665"
     CDS             19509..20594
                     /gene="uxuA"
                     /locus_tag="IVK93_001665"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6386957.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20587..21300
                     /locus_tag="IVK93_001666"
     CDS             20587..21300
                     /locus_tag="IVK93_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6386958.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7398 a         3301 c         4490 g         6311 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa acttttttaa
     1501 attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc
     1561 cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc
     1621 acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga atatattttt
     1681 tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga ttgttttatc
     1741 catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct cagatttgta
     1801 tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat aatagctttg
     1861 ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt tttttagttt
     1921 aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa aattcatttt
     1981 tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct tggagaaatt
     2041 ttccccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg aaaatagaat
     2101 gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat ctttatctgt
     2161 taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa agagtttctt
     2221 atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg gaccgagttt
     2281 tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt ttccgtatac
     2341 atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat ttgatttaac
     2401 ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg ttgcaatcgt
     2461 ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa ggcacataat
     2521 tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag tctaacagaa
     2581 gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt agttcgtaaa
     2641 aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa gaaagaaaag
     2701 ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc ggttgattgg
     2761 atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata atgcacttgc
     2821 ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg tcggacattt
     2881 agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa gaatagaaga
     2941 ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc tcgttgttgg
     3001 aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt atcggttaga
     3061 ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta ttgcagtggg
     3121 ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta gatttggtcc
     3181 gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat tgaatcctaa
     3241 catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca gcgcacttat
     3301 ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga attggacaca
     3361 aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt tctccactct
     3421 gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag catctatatc
     3481 aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat ataaaacaaa
     3541 gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa ggctgatatc
     3601 ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat tcaagcgaat
     3661 acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca ttttcatttt
     3721 atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa tgaagaaaac
     3781 agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga ttggaatcag
     3841 tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa ctgatcggat
     3901 tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca aaagcgtgaa
     3961 tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa gttcagaaaa
     4021 agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg agtatacaat
     4081 aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag ttgatttgat
     4141 tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca gttggacagt
     4201 tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag aaaacacgag
     4261 cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca cgagcagcga
     4321 aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca gcgaaagcag
     4381 tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga gtagcacagg
     4441 aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt tggtacctat
     4501 cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg tgctaaaaca
     4561 gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa cagataatag
     4621 aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta catggatggg
     4681 tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc atcgagtgaa
     4741 ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt gagtgaatat
     4801 ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc tatttttgtt
     4861 tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata agagcaagaa
     4921 taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac gaatctattt
     4981 gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag gagtatgtac
     5041 tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt tgaactattg
     5101 ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc taaactacac
     5161 cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa ggtatccaga
     5221 atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt cttctcagtt
     5281 tttactgagt aaagtttatt ctatactttt agagggaacg attggttata ttttactgga
     5341 ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa ttatataagt
     5401 ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa ctatcattta
     5461 tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat ccgatacttt
     5521 tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt atcaagtata
     5581 aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac gcgtagtata
     5641 gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa acaaggccac
     5701 tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat aacaaaagaa
     5761 gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc gatgaatcac
     5821 atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa cacctatttg
     5881 gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg gattgaatcg
     5941 tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc agaaaaaaat
     6001 tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg gacaaaaaag
     6061 aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat atttccagtg
     6121 atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc agagcttaat
     6181 acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt cagggatatt
     6241 tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg taaaacacag
     6301 gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa atatagtgca
     6361 tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa aaaggcatat
     6421 gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa tgattgggcg
     6481 cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat attggaaaaa
     6541 ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac attgcaaaaa
     6601 gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg agctcttgct
     6661 ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca gtcaaaagtt
     6721 ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta cgaatctcaa
     6781 tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta taactaatag
     6841 aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag tggataaatt
     6901 catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata tcatggacga
     6961 taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac aatttaatca
     7021 tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa atagtcgaaa
     7081 agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa ctagctagat
     7141 aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa acacagccga
     7201 cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc cgatacacga
     7261 attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa cgattgggaa
     7321 accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa aaccatggga
     7381 gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa cccatgcgaa
     7441 ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc attcgatcga
     7501 attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg ctcatggatt
     7561 aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc cttttatgaa
     7621 agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc tgacattttt
     7681 ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt tatccattct
     7741 gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata aaccatttat
     7801 tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata aaaaggagtg
     7861 aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt tgctgtaagt
     7921 acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc ggaatctcgg
     7981 gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag tacttcacaa
     8041 acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga tcaagaaacg
     8101 accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat gtcaaacgac
     8161 gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga tgatatcggt
     8221 aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca gatgacagaa
     8281 atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt agtcactgag
     8341 cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat tgctacttat
     8401 ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct tggaggtaca
     8461 gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa acaacttcat
     8521 caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata ttatgtcgat
     8581 caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg gaataacggg
     8641 atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt atttgttgat
     8701 tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata tgagtcagat
     8761 gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa gattttactt
     8821 tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga tattcctgtc
     8881 aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca actacgtttt
     8941 acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca gttagacatc
     9001 attggggatc aagagcatga agatttattt atgggccttt tgaaacaata ctattttgat
     9061 caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc agattcattg
     9121 cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa cgcgcaaaaa
     9181 ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc agggcatgga
     9241 aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt tttgaaagta
     9301 gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag tctactcaaa
     9361 gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac agagcaaaag
     9421 acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat ttttaatgag
     9481 acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc aaaaaagcaa
     9541 gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga tcaatcaagt
     9601 gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga tgcaaaaaaa
     9661 agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc aggtggattt
     9721 ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta aatgaatgaa
     9781 atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt tagtcactgt
     9841 atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa aacaatttga
     9901 cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa aaatcgaaca
     9961 aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata atatggatat
    10021 ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc atggaggcgc
    10081 atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat gttcagacac
    10141 gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac atcctacacc
    10201 agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag atcaagcaga
    10261 ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg gagaatattt
    10321 gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga ctttggagaa
    10381 caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg cacttactca
    10441 agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt actttgataa
    10501 taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac atgcggatcg
    10561 ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga aacaaatgga
    10621 agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag attatgcgga
    10681 gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac aagcgcaaga
    10741 aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc aaaagaggtt
    10801 gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag cttttcatgg
    10861 tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca ccttttattc
    10921 gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat tttataagtc
    10981 agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg gaggcaagaa
    11041 gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt ccgttgttat
    11101 tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta gcgaaaaaaa
    11161 gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt gtatgataaa
    11221 tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat gatcatctag
    11281 cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat cagataggta
    11341 caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat aatagaaaag
    11401 atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa cttttttatt
    11461 ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta aaatgaggcc
    11521 aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa ttttttagaa
    11581 aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac
    11641 tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc
    11701 gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa
    11761 aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc
    11821 tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac
    11881 gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt
    11941 gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa
    12001 aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt
    12061 ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt
    12121 gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat
    12181 ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag
    12241 ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa
    12301 tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg
    12361 aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat
    12421 tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat
    12481 ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc
    12541 ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa
    12601 tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta
    12661 gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc attttttccg
    12721 ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg
    12781 taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta
    12841 tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa
    12901 aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa
    12961 gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga
    13021 aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac
    13081 tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct tattataaag
    13141 attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga
    13201 cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag
    13261 aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt
    13321 atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg
    13381 gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg
    13441 acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg
    13501 aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt
    13561 ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc
    13621 cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag
    13681 caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg
    13741 agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt
    13801 ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa
    13861 gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt
    13921 gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc
    13981 cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc
    14041 gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat
    14101 ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg
    14161 gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt
    14221 gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt
    14281 ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta
    14341 gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa
    14401 gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta
    14461 tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc
    14521 ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat
    14581 ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa
    14641 tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt
    14701 cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg
    14761 tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt
    14821 caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa
    14881 cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat
    14941 ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa
    15001 tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct
    15061 agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag
    15121 taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg
    15181 gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat
    15241 tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga
    15301 atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca
    15361 agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga
    15421 tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt
    15481 tgctggttta gttggaggat taggagataa atacgcaaga aatgtggcag cacatgctat
    15541 gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt
    15601 agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt
    15661 atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc
    15721 gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca
    15781 tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg
    15841 ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt
    15901 tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt
    15961 tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag
    16021 ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc
    16081 caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat
    16141 tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg
    16201 gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca
    16261 aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg
    16321 ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat
    16381 tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag
    16441 taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag
    16501 attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata
    16561 ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc
    16621 aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg
    16681 aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga
    16741 taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag
    16801 atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact
    16861 atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc
    16921 atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac
    16981 tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat
    17041 cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg
    17101 aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc
    17161 ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta
    17221 tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac
    17281 aggcctaaag caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt
    17341 gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc
    17401 gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt
    17461 acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac
    17521 acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata
    17581 aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt
    17641 gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt
    17701 tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag
    17761 ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga
    17821 atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga
    17881 cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa
    17941 tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa
    18001 attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc
    18061 tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc
    18121 ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga
    18181 atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct
    18241 ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga
    18301 tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt
    18361 tatctgtact tattacgata cacccgttca tgcattagga agttatttca tgtttgattg
    18421 gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag
    18481 tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct
    18541 atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa
    18601 agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac
    18661 gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga
    18721 aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat
    18781 tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt
    18841 ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta
    18901 tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg
    18961 agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa
    19021 aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa
    19081 gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga
    19141 aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa
    19201 attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag
    19261 tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac
    19321 ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct
    19381 tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac
    19441 tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg
    19501 ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa
    19561 aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc
    19621 ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag
    19681 gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag
    19741 atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg
    19801 gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt
    19861 ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga
    19921 tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg
    19981 gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga
    20041 ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg
    20101 aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt
    20161 ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc
    20221 catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag
    20281 ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt
    20341 ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata
    20401 tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc
    20461 atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa
    20521 tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag
    20581 agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact
    20641 ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt
    20701 aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc
    20761 attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa
    20821 gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta
    20881 tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa
    20941 gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg
    21001 gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg
    21061 aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca
    21121 aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat
    21181 aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat
    21241 gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa
    21301 taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat
    21361 gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa
    21421 caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga
    21481 atcgtccata tgagggtttc
//
LOCUS       DACCCR010000044        21310 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCCR010000044 DACCCR010000000
VERSION     DACCCR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.046"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            68..808
                     /locus_tag="IVK93_001667"
     CDS             68..808
                     /locus_tag="IVK93_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6386959.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="IVK93_001668"
     CDS             complement(1232..1402)
                     /locus_tag="IVK93_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6386960.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="IVK93_001669"
     CDS             complement(1596..1775)
                     /locus_tag="IVK93_001669"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386961.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="IVK93_001670"
     CDS             2162..3274
                     /locus_tag="IVK93_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6386962.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="IVK93_001671"
     CDS             complement(3352..3645)
                     /locus_tag="IVK93_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP6386963.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="IVK93_001672"
     CDS             3813..4127
                     /locus_tag="IVK93_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP6386964.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="IVK93_001673"
     CDS             4148..6127
                     /locus_tag="IVK93_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6386965.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="IVK93_001674"
     CDS             6140..6466
                     /locus_tag="IVK93_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP6386966.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="IVK93_001675"
     CDS             6482..7000
                     /locus_tag="IVK93_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6386967.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="IVK93_001676"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="IVK93_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6386968.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="IVK93_001677"
     CDS             complement(8436..9941)
                     /locus_tag="IVK93_001677"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP6386969.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="IVK93_001678"
     CDS             complement(10033..10623)
                     /locus_tag="IVK93_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6386970.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12449
                     /locus_tag="IVK93_001679"
                     /pseudo
     CDS             10802..12449
                     /locus_tag="IVK93_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            12498..13217
                     /locus_tag="IVK93_001680"
     CDS             12498..13217
                     /locus_tag="IVK93_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6386971.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13696..14643
                     /locus_tag="IVK93_001681"
     CDS             13696..14643
                     /locus_tag="IVK93_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6386972.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14655..15575
                     /locus_tag="IVK93_001682"
     CDS             14655..15575
                     /locus_tag="IVK93_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6386973.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15616..17073
                     /locus_tag="IVK93_001683"
     CDS             15616..17073
                     /locus_tag="IVK93_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6386974.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17418..18032
                     /locus_tag="IVK93_001684"
     CDS             17418..18032
                     /locus_tag="IVK93_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6386975.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18025..19749
                     /locus_tag="IVK93_001685"
     CDS             18025..19749
                     /locus_tag="IVK93_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6386976.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19746..21197
                     /locus_tag="IVK93_001686"
     CDS             19746..21197
                     /locus_tag="IVK93_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6386977.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6869 a         3608 c         4317 g         6516 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtattttgc
    11281 aaggcaatca agaaatgatg ccttatgcgc tgtcgcagat tgtagaacag gtggcacgta
    11341 ttttttatat gctattggct acattcatca tcatgaaagt catggatggt gactatgtga
    11401 cggcggtcac gcaatcaact ttcgcggcat ttatcggtat gctagcaagt attcttgtcc
    11461 ttttgtattt tttgaagaaa catcaagcgt atacatcggc gtttattcac tacagcgaaa
    11521 acaaaataaa tattgcaacg aaggaattgt tgttagatac cgttaaagaa gcgattccgt
    11581 ttattattgt gggttcaggg gtaacgatct tcaaattagt cgatcagttc acttttatga
    11641 agatcatgtc tgcatccacc gagtattcca atgcacagtt actggatctt ttttcgattt
    11701 ttagtgccaa tccagataag ttgacgatgg tagttattgc attggcaaca tcgatcgcgt
    11761 ctactgggtt accgttgatt acagaagctg ttacgttgaa agatcgagta ggtctggcaa
    11821 aattgacaag cggaaattta cagttgtttt cattttttat gtttccggcg acatttggtg
    11881 ttatgttact tgcctatccg ctaaatacat tattttatac accggattct ctaggaagta
    11941 atgtactgat ccaagcaagt tttgtaggac tcttcctagg actatatatg ctagtttcca
    12001 acatgctgca aggaatgttt gaaaacaagg cggcaattaa ttatttggtt attgggtttc
    12061 tagtgaaact ccttcttcaa tacccagcca ttcgtttatt tgaagtatac ggccctttat
    12121 tggcgacaat gatcggattt gctgtgtctt gtaccttgat cttaaagaga atccatgaag
    12181 tagcccgctt taaaccaagt tttgtctggc gcaggacatt gctgatcttt attcttacct
    12241 tgattatgct tttggcttcg tggatcacaa agatgatttt tggtgctttt ttaaacgaag
    12301 atagcaaatt ccagtctcta ttgttgattc ttgtagtagc aggtataggg gctttagtgt
    12361 atggttattt agctttgaaa atccgcttgg cagaaagact actaggacca ggaatagcgc
    12421 gtttacgaca taaattaaaa ataaaataag tgaatcagaa gagctagatt gatgctcttt
    12481 tgaaatgggg gaaacgcatg cgtttagata agtttttagc agatatggga cttggcagcc
    12541 gcaaagaagt caaaggatta atcaaaaaag gaacgatcga agtcaatgga caagtggtga
    12601 aaagcgataa agcgcaagta aatgaattcg aagatgcggt tacttattta ggagaaatca
    12661 ttacgtatca aaaagatttt tactacattt tgcataaacc tgcaggagtc atttctgcta
    12721 ctcaggataa tcatgatcaa accgtaatcg atttattgga agaccaggat tatcgggaag
    12781 atctttttcc agtcggccga ttagataaag acacagaggg cctcttgatt ttaacgaatg
    12841 acggggtgtt tgcccatcag ttactatcac ctaaaaaaca tgtggagaaa gaatatctag
    12901 ctgaggttag cggtataatg acagaagaag atatccgttt atttgcagaa ggactgatta
    12961 ttgatggcga tgagcagaca ttgccggcaa aactccaaat tgatcaagta gatcaagaga
    13021 cagaaacttc aattatccgt ctgattttgc atgaaggaaa gtttcaccaa gttaagcgaa
    13081 tggtcaaagc agtaggaaaa gaagtcttat atttaaaaag aatccgtatg ggaaaatttt
    13141 ggctgcctaa agatttgaat aaagggcaat atcgacccat gaccaaagaa gagctagaac
    13201 aagtgagaga aaaataattg aatggatagc taaaagaata agcagagtag ttgtagcgcc
    13261 atctgaaaag cagacttgtc cgtctgactt tcggatggct tttttgtgaa atttcggctt
    13321 atttccgtag aagctacttc tggaacaccg tttgttcgga ataagggact gtgacaagac
    13381 ttttgttaca gtccccattg ttccttgtga ctcagagtta gatagtaagg acacgatctc
    13441 cctaatactc aggccaaaac gctgaattca caacctctga taaacggtgt tcaatcagct
    13501 gaccctcggt attagctcaa aaaacggcaa aatatgagga actattttcc cgtttttttc
    13561 tctaatactc aggccaaaac gctgatttca caacctctcc tatactttgt ttcattttcc
    13621 ctatactttg tttatttatt ctcattattg aaagcgctat aattacgttg cgaatgaatc
    13681 gaaaggggtt tttagatgaa aaaacgaaag gggttcaatc atttttttat gaatctatgg
    13741 cgttataaag cacttgtcct gatggctgta ccgggtatgc tttggatgat ttttttcttt
    13801 tacatacctg taatggcgaa tgtcgtagca ttcaaaaatt ttcatatttc agcagatggg
    13861 ttcttggcta gcttgaggga aagtccttgg gtgggttggg aaaatttcaa atttttattt
    13921 tcttcggatc aagcatttct catcactaaa aatacaattt tatataatgt agcatttatc
    13981 cttttgaatc tgctgatctc agtcgtattt gcaatcatta tgagcgagtt gagaaataaa
    14041 cgactcgtta aagtttatca aacgatgtcc ttactacctt attttctatc ttgggtcatt
    14101 atcagttact ttgtttacgc ttttttaagt cctgacaaag gaatattcaa tcagtggatc
    14161 actggacatg gaggagaagc tgtcaattgg tataatgaac cgaaatattg gccgtttatc
    14221 ttggtgttca ttggtacatg gaaagggatt ggttataaca gcattattta ctttgcatct
    14281 gttatgggaa tcgatccgac ttattatgaa gctgctatgg ttgatggagc tagcaaatgg
    14341 cagcagatca aacatgtgac gataccacaa cttattccac taatgacgat tttaacgatt
    14401 ttagccgtag gaaacatttt ccgagctgat tttggcctgt tttataacat tcctaggaat
    14461 tccggtgcat tatatgatgt gactcaagtt ttggatacgt atatctacaa tggcctgaca
    14521 tccacaggag attttggtat gacggctgct gcaggacttt accaatcagt tgtcggcttt
    14581 gtcttgttga tgatcacaaa tacgatcgcc cgacgttttg atagcgattc tgcattattc
    14641 taaggaggaa aaaaatgaaa aaaaagaaag tggagagagt ccaagtccgc tctttcaata
    14701 agacggctaa ttttttctct aatataatga ttgcactctt tgctatttcc tgtattttgc
    14761 catttatatt tgttatcgcc atctctttta ccgatgagtc catattgtcg actcacgggt
    14821 acagtttttg gccagaaaag ttcagtatat ttggttatac gtacttgttc gaccagatgc
    14881 aggataaaat tttccaagcg cttttcgtca ctgtacttgt tactgtttta ggtacactga
    14941 tcaatagcac ggctacttct ttatatgctt atgcgatttc acggtcaaac tttccattcc
    15001 gtcgtttttt taccgttttt tgtttgatta cgatgttgtt ctcaccaggg atggtggcca
    15061 attatcttgt tatgacaaat cttcttcagt taaaagatac gatttgggca ttgatcttgc
    15121 caatggctgt cagtcccttc aatattattg tgatgcgcac attctttaaa cgttcggtct
    15181 ctgattcgat tattgaatcg gcacggatcg atggagctag cgaactgcga atatttgttc
    15241 agatcgtttt accgttagct gttccaggta ttgcgacaat cagtttattt gccgcattgg
    15301 gttactggaa tgactggttc aatgctttac tatacatcca agatgataag ctagttccac
    15361 tacagtatct tttgatgaaa atccaaagta atattcaata tctgacacaa aattcaggag
    15421 caggaagcca attgacaggc ggattagcct cagttccagg agaatctgcc agaatggcga
    15481 tcgtagtcat ctcgacgttg cctattgcga tcagctatcc atttttccag aagcattttg
    15541 tcaaaggtct aacaatcggc ggagtaaaag aataaagaaa caaaaatata aatcataaaa
    15601 ttaggaggaa gaaaaatgaa gttatggaag aaactagcct ttacaggtgt ttcagcgttg
    15661 ttgttgggaa cattagcagc atgcggaggt tctggttcta aagaccaggc agaagctagc
    15721 aattcggaca ctttgcagat gtatcaaatc ggagataaac cagataattt cgatcaattg
    15781 atggaagtag caaacaaacg aatcgaaaaa gagatcggtg taaaagtcaa tatcaattac
    15841 attggctggg gcgattacga aaagaaaatg aacgtcatca tttcttccgg ggaaaattat
    15901 gatatcgcct ttgcgaacaa ctatgtttcc aatgcccaaa aaggagcctt tgctgatttg
    15961 actgaattag cgcctaagta cgctgaaaaa gcttataatg atttagatga agcatatatt
    16021 aaaggaaatc tggtcaatgg aaaattatat gctttcccag tcaatggaaa tgtctttgct
    16081 caacaagtac ttacttttaa caaagcttta ctagataaat acgatctttc aattgatgga
    16141 atccaatctt acgctgatgc agaaaaagtc ttgaaggaat tccatgaaaa agaaccaaac
    16201 acggcagctt ttgccattgg acaaggcttc aaagtccaag ggaactttga ttatccttta
    16261 gggaatacgt taccatttgc tattgatctg gatggagatg attcgaaaat catcaaccaa
    16321 tacgataatg aaacgtatat cggactgtta cgtacaatgc atagctggta caaacaagga
    16381 ctcattccaa gtgacgctgc aacgagcaat aaggattatc cactagaagg aaatacttgg
    16441 ctaatgcgtg gagaaacaca aggaccgtat gattatggcg atacaatttt gacaaatgca
    16501 gcaaaacaag aacttgtctc aaaagcaatt actgttccac taaaatctac agctcaagca
    16561 caaatggcaa attttgtcgt atcaaatact tcaaaaaata aagaaaaatc agtagaactt
    16621 ctagggctgc ttaacagtga tccagaatta ttgaatgggc ttgtttgggg gatcgaggga
    16681 gaagcatggg aaaaagtcga tggttcagat gataaaatca aattgctaga tggttatcag
    16741 ccaaatacac atatgtctgc ttggaataca ggaaacaaca agattctata tacacaagag
    16801 tctattacgg atgacatgat tgccaaacgt gatcaatcga ttgctgatgc agaaacttca
    16861 ccgattttag gattcagctt caacactgac agtgtcaaaa cagaattaag taatatttct
    16921 aatgtcatga atcaatattt agatggattg aatacaggga ctgttgatcc agatgaaaca
    16981 ttaccgaaat tgaaagacgc tttggacaag gctggctatg ataaggtatt gaaagaaatg
    17041 caaaaacaat atgatgagtt ccgacaagaa taataatgaa taaagacaaa ggggttgtga
    17101 caaaagacgt gtcacaacct cttgttccga ataaacggtg ttcaaaagcc agtttctcgg
    17161 tattaagcta aatatatgta aaaatgtgga aagccatatt cacaaaattt ttcttactac
    17221 tcgaaactaa gcggctattt cacaaactct tggtaaacgg tgttcaatca gctaaccctc
    17281 ggtattcgct cacaaaacga caaaatatga ggaactattt ttccgtttgt ttctctaata
    17341 atcaggtcaa ggtgctgatt gcccaacctt tttttgtata atgagaaagt ataaacttaa
    17401 tgtttggagg agaagatatg ttagggaagg ttttggaaga gttatttatc cggatctggg
    17461 tcattataaa actaaccttg tatttctgga tatatacatt tgccggcggg atcatctttg
    17521 gattaggtgc ggcatggaaa acagtcaatg aactatttta tttgtacgga ttcgagtata
    17581 aagaaatcac aataaaaaga ggctggaata tatataaaag aaatttttta agaggcaatc
    17641 tgctatttag tttattttta tctggtactg ctttattgag ttacaattta tatttatctg
    17701 ttcaaatcaa agggctcatt tttttagcta ttgattttat tttacttttt gccttgctct
    17761 gcctatttgc tacttatcaa tacagtatga ttttagatag tgaatatacg atctctgttc
    17821 ctaatctgct aaagctaagt tttatttcgg ttttttccag cttttcgtct tttctaaaaa
    17881 taattattgg tagcggtata attttaggcg tgacttggca attcaaagga ttgatcctgt
    17941 ttggtgtcat tggtcttttg acggtttgga atggtacgat gacaacacat tggcgtgaag
    18001 aattagataa acagttggaa agttatgaat aagtttcgga ttctattttc aaaaggaacc
    18061 ttactaaatc gtctgttgcg gaaatacgcg gttatcatga tcggtgtaac gatgactgct
    18121 accgttattt ttagtatcca tacttgggaa caaaatcaaa aacaggcaga aaacatgaca
    18181 agtgatgccg ttcaaagcac aagcagaatg ctgaatgata aaacaacctt gagtaggatc
    18241 atcaaaaacc agcttgtagg gaattccgaa aagatcgaaa atgtgacgac ctacttaaca
    18301 aaacccatag atcagtatct gatgtatgta tacgaacagc aaaacagtac cgatgaatta
    18361 gtttctttcc caaatcaaat caaagatctc tacgcaaatt atgaagaact aagtgcgata
    18421 tatatcgtgt tgaatcaact accggagtat tatgaatctt ctagagaaaa caaaggtggg
    18481 gaaataaaaa aggggacacc aaaattacta gacgcctttt atatcaagtt gcctatcaca
    18541 caaggaggag ctgagtcagg aagtattttt attggttttc aaaagaaaga actagatatg
    18601 attttagaaa atctaacgtc attcaatgga ctctctttat acatgatttc tggaacgaca
    18661 aatcgactat acacattcca tgataataaa atagctaaag aaacgtttga aagacaagaa
    18721 atgatcatca cagagagtct gaagaaaaat tcacaattgc cgattgacga tctggaaaaa
    18781 aacaattttg ttcaacacca agaactgtct gatgattatc gtatattagc tgtactagat
    18841 aaaaaaagtg taaaaatcga aacgggaaaa aatcttgccc cgctgatcat tggtatcatt
    18901 attttagaca atatactgct tatctttctt tataaaacat tcaaacgata ttcacagcaa
    18961 gtatctatcg tcatgcaggc catggatcaa acagcagcag ggaatttaga gacgcggatc
    19021 gacataacaa acacggaata tgaactgaag gaactttcaa tcggtatcaa cgaaatgttg
    19081 gatagcatca atcagtttgt agaagacatc tacaaattag aaatcaaaca gcaagacgca
    19141 catatgcggg cgcttcaagc acagatcaat ccacactttt tatataatac attagaatat
    19201 atccgaatgt atgcaatcag cgaaggaagt gaagaattag cagatgttgt gtatgcgttt
    19261 tccgctttgc ttcgaaacaa tacgaatcaa gagaaaacga tcactttaaa agaagaactg
    19321 gacttttgcg aaaaatatgt ctatttgtat caaatgcgtt atcctaatcg agtagcctat
    19381 cattttatga tcgatcctga tttggaaaag atcgaagtac ctaaatttgt gatccagcca
    19441 ttagttgaaa attattttaa acacggtatt gattttactc gattcgataa tgcgctaagt
    19501 gtcaaagtct tgcaggaagg caaacgggta aggatcatca tcaaagataa tggaaaaggg
    19561 atgactgaaa aacggctaaa acagattgaa gaaaaactgt ctcatcctaa agtggaactt
    19621 cacggttcta tcggtctgca aaacgtgaat gaacgtttac gtgccagctt cggcagcagt
    19681 tattatatgt cgcttgagaa taatgagaca ggtggactga ccgtttctat tacattcaag
    19741 gagggatgag cgtgtatcaa gtgttattgg tagatgatga atacatgatt ttgaatgggt
    19801 taaaaaagat aattgattgg caaagtttag gctttcaaat agtagcaaca gcagagaatg
    19861 ccaaagaagg actagctgta cttgaacagc gacagattga tttagtagta acggatgtga
    19921 caatgccaga aataaatgga ctggaattta ttgaagcggc tcaaaaagaa cgtcataatt
    19981 ttgaatttat gattttatca ggataccaag aattcgatta tttgaaagga ggtatgcaat
    20041 taggcgccgt gaattatttg atgaaacccg tgaataagtt cgaattggtt gaaagcctca
    20101 agaaaatcaa aacacggtta gatcagcaaa atgaacaaaa aaatcaacaa gaaatctatc
    20161 aagaactgtt attcagtcaa tggttgaatg aagaattgga tgaaataagt gaagaagaaa
    20221 taataggaca aataagtgag aagcagcata ggatagtatt gattcaactg gcaagaactc
    20281 atgatactct tatcaataga tggttgcaag aacggcaaca aatcttttac tatcaacgaa
    20341 attacggaga ccgtgtattg atcacgttgt tgttggaggc agatgaagtt gatggcttct
    20401 gttgttttgt tcaagaaaac tttcctgatc aggaatggct gatcagtatc ggggaagaga
    20461 cagattcttt ggacaaaatc cctgaaacat tccaacaagc gaaagacaat ctacagttgc
    20521 atcaatttta tggagataaa gacgagcatg ttttttacgc agtacaagca gaaacaaaag
    20581 atcaaaccat cgatttttca agtttcagac gcgtgctgca aaataaacaa ttggaagtcg
    20641 ctcaaaaaat gatcactgat ttttttgaac agtttcagtt agcagccatg atgccggaag
    20701 atatacgata ttccagtttt ttactgttta tggagattca acgggaattg attgatctgg
    20761 aagatgagga atatttacaa ggaatcgaaa aaatccatca ggcaaaaacg gtgcaagaac
    20821 ttcagcagct acttctgtca ttcatcaaaa agcatcagag acagaaaaag tacagtgaca
    20881 atgtggaaaa agtaatcgta atcctgcacc agcattatca agaaccactt acactcaaag
    20941 aagtgtcaga aaacctgcat ttaaatgtca tgtatctagg acagctgttc aaaaaagaaa
    21001 cgaaaaaaag tttttcagct tatttgaacc atttacgtat ggaaaaagcg aaacaactgc
    21061 ttttgcacag taatcaaaac atcaatgaga tagcaagtga gattggctac aacaacacca
    21121 cgtatttctc taaattattc aagaaaatcg tcggcagatc ccctaaagag taccgagaaa
    21181 acagtggaga atcataaaag aaaaagccaa tgtaatgaaa taacttataa aaaattgtct
    21241 agaaatctaa ttttgggctg ttcttattac ttgaggcaag gcagcttttt catgacctct
    21301 cgataaacgg
//
LOCUS       DACCCR010000045        20361 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCCR010000045 DACCCR010000000
VERSION     DACCCR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.052"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            292..1431
                     /locus_tag="IVK93_001687"
     CDS             292..1431
                     /locus_tag="IVK93_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP6386978.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IVK93_001688"
     CDS             complement(1498..2073)
                     /locus_tag="IVK93_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6386979.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IVK93_001689"
     CDS             complement(2078..3052)
                     /locus_tag="IVK93_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP6386980.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IVK93_001690"
     CDS             3335..4303
                     /locus_tag="IVK93_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6386981.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IVK93_001691"
     CDS             4416..4694
                     /locus_tag="IVK93_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6386982.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IVK93_001692"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IVK93_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP6386983.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IVK93_001693"
     CDS             5160..5906
                     /locus_tag="IVK93_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6386984.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IVK93_001694"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IVK93_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP6386985.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IVK93_001695"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IVK93_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP6386986.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IVK93_001696"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IVK93_001696"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP6386987.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IVK93_001697"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IVK93_001697"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP6386988.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IVK93_001698"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IVK93_001698"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP6386989.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IVK93_001699"
     CDS             10643..11185
                     /locus_tag="IVK93_001699"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP6386990.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IVK93_001700"
     CDS             11208..12764
                     /locus_tag="IVK93_001700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP6386991.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IVK93_001701"
     CDS             12778..13680
                     /locus_tag="IVK93_001701"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP6386992.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IVK93_001702"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IVK93_001702"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP6386993.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IVK93_001703"
     CDS             15123..15545
                     /locus_tag="IVK93_001703"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP6386994.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IVK93_001704"
     CDS             16014..16244
                     /locus_tag="IVK93_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP6386995.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IVK93_001705"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IVK93_001705"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6386996.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IVK93_001706"
     CDS             17655..17831
                     /locus_tag="IVK93_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6386997.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IVK93_001707"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IVK93_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP6386998.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="IVK93_001708"
     CDS             18326..18709
                     /locus_tag="IVK93_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6386999.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="IVK93_001709"
     CDS             18878..19762
                     /locus_tag="IVK93_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6387000.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IVK93_001710"
     CDS             19879..20112
                     /locus_tag="IVK93_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP6387001.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="IVK93_001711"
     tRNA            20200..20280
                     /locus_tag="IVK93_001711"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="IVK93_001712"
     tRNA            20287..20358
                     /locus_tag="IVK93_001712"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta t
//
LOCUS       DACCCR010000046        20047 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCCR010000046 DACCCR010000000
VERSION     DACCCR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.053"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..2034
                     /locus_tag="IVK93_001713"
     CDS             <1..2034
                     /locus_tag="IVK93_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6387002.1"
                     /translation="RIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEELKT
                     KNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIANID
                     YISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQLLS
                     DLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSARFLV
                     SRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIFESK
                     TTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNLSLG
                     VYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHNKLN
                     KQRQRAVVVCTNGVMVSKLVRVNMEKLFPEIHFLASLSVRDFYKFEADYDLVFTTTPL
                     KSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPVSLR
                     EELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLLEHQ
                     IIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFPNGE
                     DVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2045..2665
                     /locus_tag="IVK93_001714"
     CDS             2045..2665
                     /locus_tag="IVK93_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP6387003.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2677..3135
                     /locus_tag="IVK93_001715"
     CDS             2677..3135
                     /locus_tag="IVK93_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6387004.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3154..3432
                     /locus_tag="IVK93_001716"
     CDS             3154..3432
                     /locus_tag="IVK93_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6387005.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3457..4824
                     /locus_tag="IVK93_001717"
     CDS             3457..4824
                     /locus_tag="IVK93_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6387006.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            4839..5984
                     /gene="dgoD"
                     /locus_tag="IVK93_001718"
     CDS             4839..5984
                     /gene="dgoD"
                     /locus_tag="IVK93_001718"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP6387007.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6011..6502
                     /locus_tag="IVK93_001719"
     CDS             6011..6502
                     /locus_tag="IVK93_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387008.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            6945..8144
                     /locus_tag="IVK93_001720"
     CDS             6945..8144
                     /locus_tag="IVK93_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6387009.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8639..9397)
                     /locus_tag="IVK93_001721"
     CDS             complement(8639..9397)
                     /locus_tag="IVK93_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6387010.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9565..10500
                     /gene="pfkB"
                     /locus_tag="IVK93_001722"
     CDS             9565..10500
                     /gene="pfkB"
                     /locus_tag="IVK93_001722"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP6387011.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10497..11942
                     /locus_tag="IVK93_001723"
     CDS             10497..11942
                     /locus_tag="IVK93_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6387012.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            11970..12425
                     /locus_tag="IVK93_001724"
     CDS             11970..12425
                     /locus_tag="IVK93_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6387013.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12444..13418
                     /gene="lacD"
                     /locus_tag="IVK93_001725"
     CDS             12444..13418
                     /gene="lacD"
                     /locus_tag="IVK93_001725"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP6387014.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13529..14090)
                     /locus_tag="IVK93_001726"
                     /pseudo
     CDS             complement(13529..14090)
                     /locus_tag="IVK93_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14234..14848
                     /locus_tag="IVK93_001727"
     CDS             14234..14848
                     /locus_tag="IVK93_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6387015.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15089..15472
                     /locus_tag="IVK93_001728"
     CDS             15089..15472
                     /locus_tag="IVK93_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6387016.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            15835..16344
                     /locus_tag="IVK93_001729"
     CDS             15835..16344
                     /locus_tag="IVK93_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP6387017.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16444..17094
                     /locus_tag="IVK93_001730"
     CDS             16444..17094
                     /locus_tag="IVK93_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387018.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17545..18483
                     /locus_tag="IVK93_001731"
     CDS             17545..18483
                     /locus_tag="IVK93_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6387019.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18496..19548
                     /locus_tag="IVK93_001732"
     CDS             18496..19548
                     /locus_tag="IVK93_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6387020.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            19606..>20047
                     /locus_tag="IVK93_001733"
     CDS             19606..>20047
                     /locus_tag="IVK93_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP6387021.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
BASE COUNT         6335 a         3188 c         4244 g         6280 t
ORIGIN      
        1 cgaattcgtg aaatactcaa actgattatt aaaaaccctg aaatgaaact ttccacttta
       61 acaagtgaac ttgatttgac acgtcgtcaa atcaattacg cgatcaatca atttaatgaa
      121 gaattgaaaa caaagaacat tccgttaatt cagagaagtc attctggaaa tattacggta
      181 ccaatcgagg ttatccaaat gatatcacag cctgattacg acataggtga tgatcaggaa
      241 cttgtcgttc tcactgatag tgaaagacaa gcattgatag tgatgactct gatcgctaat
      301 atcgattaca tcagtctgga tcatttggta gatgcggttg atgttagtaa gacgactgtt
      361 atggaagata tcaaacgaac ggaaccgttc atcagaaatt atagtctgac aattcgatat
      421 aatcggatga atggttacca attggttggt tcggaacacc gtatcttaca gctgttatcg
      481 gatctagtaa aacggtttcc gatttttcgc cgagaaatga ttcgcagaaa attggcacaa
      541 gatgtttctg aagaagaaat cgtccatttg atccataaca tggaacaaat gttgcatctt
      601 agttattctg atgagtcggt agactatttg cagacatcag cgagattttt agtaagccgt
      661 gggatcaaga tgtcagataa gcacgcgttt tttcccgatg atgtgacgga tactcctgaa
      721 ttccgaatgc tgatgatctt ggtacatgaa acaggttgga gtctatccaa aagttatatg
      781 gagtggttca cgttattatt tttaacttcc aatatttttg agagcaaaac tactcaaaat
      841 tatgattcgg atcaagaatt gcgaaaattg atcagccaaa tggttgaaaa tttccaaaat
      901 caaacgttga ttttgatcga tgaccgagaa gactttgaac gtaggatact gagtcatctg
      961 cgtccagcct gttttcgaat acgctataac ttgagtttag gagtttattc aatagatagt
     1021 ctgatcaagg atagtaatca tgcgattttg attgatttga tgaaagaatt gattattcct
     1081 attgagaatt ggctaaataa ggcctttcca aatgatgaat tagatctttt atcctattac
     1141 tttggctatc aattaagtaa tcataataaa ttgaataagc aacgacaaag ggctgtagtt
     1201 gtttgtacta atggtgtcat ggtctcaaag ttagtacgag tcaatatgga aaaacttttt
     1261 ccagagatcc attttttagc ttccctatct gtgcgtgatt tctacaaatt tgaagctgat
     1321 tatgatctgg tctttactac gacgccacta aaaagttcta tgctgcagtt tatgatcgag
     1381 ccaatcatga catatcagca acaaatcagt ttacgatatc gagttttgag tgatttagga
     1441 attactaagg ttgatcgagc aatggatgaa ttgttaacaa tcattaggaa atatgcaaat
     1501 atcaccaatc cagtttcgtt gcgagaagaa ctgcagtact ttttgatcaa ggaagatgga
     1561 agcacgccgc tggacaactt caatgtattg ccttcattga cttattactt gcggccgagt
     1621 tatgtacaaa tcgttgaaga agagatgtct tgggaacgag cagtggaggt ggcttgccga
     1681 ccgttattgg aacatcaaat cattgactcg cgttttatcg ctgattgctt ggatcaaatc
     1741 agacaaccgg gatatgcagg ttacttggga atgaagactt gcattcccca tacaacggtc
     1801 gataaaggtg taatcagtga cggtgtcagc attttagttt gtaaaaagcc gatacgattt
     1861 cctaatggtg aagatgtcag cttgattttg ccgctttcat tttttgattt aacgaagcat
     1921 ctgcgagcca tcaatcaaat cgctgatatc gccaaagatg attcattgat tgagcagtta
     1981 ttgattgctg atgacgaaaa gacagtttat caactattaa gacaaagatc ttaaggagga
     2041 aaacatgaga ctagcagatt atcctaaatt gacagtgatc atgcgtggtt atacttttga
     2101 tcaggcagat gctattttac aagcaatgca ggaatttgac catcaatttg ccgtagagat
     2161 gacaatgaac acaaaaggag ctcttgaaaa tatacatgaa ctaaatcaac gttatggtga
     2221 cacgacttat atcggcgctg ggacggtaag gacattagaa caagcccaag cctgctatga
     2281 ggctggtgca aaatttttat taggaccaca tatgttcaca aaagaaatgt tgtcttacgc
     2341 caataagaaa ggattattgg cggtacctgc cgcaatgact ccttcagaag tcgaccgtat
     2401 gtttaaagaa ggagctgata ttgtaaaagt atttcctgca gcggttgtta ctccacgttt
     2461 tttcaaggat attcaagcac ctttaggtaa gttaccacta atgggggttg gtggaatcag
     2521 taaggagaat gcgaaagagt tttttgaaca aggtgctagc tatttaggtc ttggttccgg
     2581 tatgttcaac aagcaagaca tcgaagagct aaatgttaaa aacttagcac gttcgatgaa
     2641 agaattaatt gaggctatta aataggagtg aggactttgg aactttattt gaatgaggaa
     2701 ctcgtttttc gaaatgttca agcaaaaaca gatagtgaag tcttagcgtt tttagcaagc
     2761 gagatgtaca aaaaaaacta cgtgaaggag gaatatattc aagcaatcca agaacgggaa
     2821 aaagaatacc ctactggatt accatcaaca ccaccaggaa tcgcaattcc ccatgcaaac
     2881 tatgaaatgg tcaataaaac aactttagca attgcgactc ttaaagatcc ggtcctattt
     2941 cataatatgg aagataacaa tgagcagtta ccaatccaaa ttgtgatcat gatggcgatt
     3001 ggggaaccac atgggcaagt tgaaatgtta caaaaaattg ttggaatcat acaagatgag
     3061 ccactgcggc aagaaatgat cagagcaggc aatgatactg aattattaga actattaaag
     3121 aaagcggttt tttaatataa ggaggaaatc ataatgaaac gaattttagt agcatgcgga
     3181 aatggtatcg ccacttcaac agtagtagca acaaaagtta gagagtattt gcaacaacat
     3241 ggcattgatg tttcaacgac tcaaacaaaa ttgatggaag ttccaggaaa agttcaagac
     3301 tatgatctat tggtgacgac tggtcaattt gatggtcaaa caggtggtgt accagtaatc
     3361 aaagggatgc cgattttaac aggtatcggt gccgatcaga caatggaaga gattttgaac
     3421 ttactgaaat aatagctgat caaaggagct gaaaaaatga aaatcattat gaacgtcttt
     3481 aacttcttta ttgatgctgg accaacagtt atgttgccag tcatcatcac gattattggt
     3541 ttgatttttg ggttaaagat ttctcgtgct tttaaatcag gtttgacatt aggtatcggt
     3601 tttgccggga tcaagttgat attggacttt atgacgacca atgttggacc agctgccaag
     3661 gcgatggttg atcgaactgg tgttaaacta gatgctttgg atgttggctg gggttcgatt
     3721 gcggctgtta cttgggcatc gccaatcatt ccaatcttga tttttgccat tctattggtc
     3781 aatatcgttt tgctgatttt gaaacgtaca catacgctgg atgttgatat ctggaactat
     3841 caccatatgg caatcgttgg tgttatagtg tattttgtga ctaagaatgt cttcttaggt
     3901 gtaggtgcat cagtagtgat ggcaattgcg acttttaaga tttcagactg gtcgcaaccg
     3961 atggtagaaa gtttcttcgg aatccctggt gtttctttac caactgtttc tgcgctttca
     4021 tcactggtta ttgcttggcc gttaaattgg ctgctggatc gtatcccgtt attcagaaaa
     4081 tcaaaattca ccatcaaaga tgcacaaaaa tatttgggat tttttggtga ttctatgatc
     4141 atggggttag ttattgggat ggtgattggt gttttagccg gctatgatat caaagcggtt
     4201 ttacaattag gtgtcagcat gtcagctgtt ttggttttga ttcctaagat gacttcatta
     4261 tttatggaag gattgatgcc aatttcagat gctgcacaaa aatggtcaca aaaaaaattc
     4321 aaaggtacac gtttatttat cggattggat gctgcagttg ttgtaggtaa tccagatgtt
     4381 atcacaacag cattgatcat tattccacta acgattgcat tagctttagt attgccagga
     4441 aaccgtgtgt taccattcgc agatttagcg gttgttcctt tccgcgtggc aatggtcgtt
     4501 gccttgactc gtggtaattt attaaaaaat atcattattg gcttagttgt gacagcttca
     4561 ttgctatggt gtgggtcagc tactgcgcca atgctgacag caattgccaa atcggttggt
     4621 attaacttag gaacttcttc aatgttaatc tcatcatttg ctgcgacttc catgatccaa
     4681 agttatttgg tatttatcgc atttgcttac aaaccaatca tcggtattcc agtgttgtta
     4741 ttagcctttg cgggcgcatg gtattacttc gatggtatca aaaaagttaa tcatgaaatg
     4801 gatgaaccat tgaaggcaca atagtaggag ggtttaaatt gaaaatttca gatgtcacag
     4861 tgtataaagt aaaaccaaga tggatttttg ttaagatctc gactgacgaa ggaatcgaag
     4921 gctggggaga aatgatctca ggaactaaaa cagaaacggt tgttgctgga gcttatgaga
     4981 taggaaaccg gctgattgga cgtaatccgt ttgaaatcga gcgcctcttc caagaaatgc
     5041 atcgatcttt cttccgaggc ggtcctatta atggaaccat cgtttcgggt ttagaaatgg
     5101 ctctgtggga tatcaaagga aaagcgttta atgttccggt ttatgaatta ttgggcggtg
     5161 cagcacgtga taaaatcaag gtttattctt ggattggtgg agatcgacca agtgaagttg
     5221 cagaacaagc acaagatcgg tttgatcggg gctttacggc cgtcaagatg aacgcgacag
     5281 aagagctgca ttatatcgat tcctaccaaa aagttgatga agtggtggaa cgagttgcgt
     5341 ctattcgcga acgttttggt gatgcaatgg atattggtgt tgattttcat ggacgggtcc
     5401 ataaaccgat ggcaaaagtg ctagcaaaag ctttggagcc attccatcca atgtttttgg
     5461 aagaagtcgt tttacctgaa aatgaagaac actttaaaga agtagctgat tctgtggctg
     5521 taccattagc tactggtgaa cgcttgtaca ctcgttggca attcaagaac ctctttaaac
     5581 aaggtactgt tgatattatc caaccagatg tggctctttg tgggggaatc ttggaaaccc
     5641 gtaaaatcgc agcgatggcg gaagcttacg acatggctgt tgcgcctcat gcgccatatg
     5701 gtcctgttgc actagcagca actctgcaag tagactcatg tacgccaaac gtctttatcc
     5761 aagaacaaag cttagggatc cactataaca aaggttttga tctgttagat tttgtgaaga
     5821 acaaagaagt tttccaatat aaagacggct acgtagatct accttcaaaa ccaggtctgg
     5881 gcttggaaat ggatgaagac aaaatcaaag aaattgcaca agaaggtttg atttggacca
     5941 atccgcaatg gaaaaattat gacggtacga tcgctgagtg gtgataaatg aaaataaagg
     6001 atgatgaaag atggcagaag cacttatgat cacagttttt ttaggactgc tgattatttt
     6061 gcaaataatc gatcaaaaga aaattatcgt gtttgctcag agtagtcctg taaggaagag
     6121 tttgagtatt ttactgacag tcgccatcct gttgattttc tggccggaac atctgactga
     6181 tcagattaaa ctggttgctt ttgcttctgt cattttgatc aatggttttt tgaaggaggg
     6241 tctagcaaaa gattctgttg ttagatttgg agtattgaac ggtaacttta atcgatatcc
     6301 tcaagtccaa gtagaaaaac taggtgacga taaatcattt atttcatttt acaaaagcaa
     6361 gaataattct ttttcaatga tttttgataa agatcaaact gaaatgatgg atgtctttca
     6421 agaacttgat atccattcca aaattacttt agatcaaagt tcggaagaaa aaagagcagt
     6481 tgtaaaaaaa gataatgaat gattctacta ccgaaattat gatttcttta tcacgaataa
     6541 tgaaacttgt tttaatgaat catattgatt gaagtataca atgtatatta agttttggaa
     6601 agaaatatat tggtacaaga tatagcagga atcgattact cgatccctgc tttttttaac
     6661 ttcctttgca gcaaaaaaag ctcgtaatct aagagatgat ggatagtttt tagaatatat
     6721 atattttctt caattccaga aaaaaatgac caagctagta tgctaaaaaa gtacgatgct
     6781 tctatgatct tttttccgtc tttattaaca gatgttttaa ttgatcaacg catattgtaa
     6841 tctaccctaa tttccatagt tcttcaccac ttttaaaaat agtaaaaccc ctacaagtat
     6901 gctatagtaa attgctaaag aaaaaccaat aaggtgggag atgtgtgcgc gatcaaacaa
     6961 gcggaaaaat gattcgtttt tattatacaa gtttgcattt tctttattgg atgatgtttt
     7021 gttctgtcta tgggtttgcg acattgtttt tgatttattt caagattgat ccagctaaaa
     7081 tcggagggat cttagcattg gtcaatattg tttcaacggt gcttcagcct tttgtcagtc
     7141 aagtgatcat caagaaaaga aagatcccac tgatcaccgt gctgaagacg atgatagcag
     7201 gattaggagt gattctctta gctggggtat tgattccgaa tttgccgatc attacttata
     7261 tcttgggtgg gattttagta gtttctatgc aatcatttct caatgcgctg gcatttgagt
     7321 attcaaatac cggctacagg atcaactttg gcttttcaag agctggtggt tcattttctt
     7381 atgctgtaac ttctttttta ctgggacagc tgttagcgat ttattctgcc gcgattttgc
     7441 caatcttggt tatagttgaa gcagtgatat ttttcgttat tttgcatctg ctgccggatg
     7501 taaaaaatca agaagttttt cctatgacaa aagaacagga tcgctcagtt tctttgagaa
     7561 aaaaatatcc tttcctgatt ttgttatttt taggtttcag ttttcttttt actttccaca
     7621 ccatgatcaa cagctttttg gcacagatct ttgaaaatat ccacggcggc agtaaagaag
     7681 ttggcatcgc attgatgatc gccgctttat gcgaattacc gggaatgatc ttttttgaac
     7741 agcttgtcaa aaagaaatcc agcaaatttt ggctgtctgt tgccagtatt tcatttgctg
     7801 tcagaggatt catgatgctg ctggccagtt cgatcctgat gatggaaatg acgcatctcc
     7861 tacaagcctt cagtttcgct ttgtttattc cagcttcggc ttatttgttc aatcaatttc
     7921 tggcggatga tgatcgtgta tttggacaaa cgatgatcat catcgccaca actcttggtg
     7981 gggtgatagg aaatgtcttg ggcggcagtc tgctgcagca tttcggtgta tgggagatgc
     8041 tgattttcgg tactggtttt gcggtgctag gtgctttatt aactattttg ggcattcgaa
     8101 aaataccgaa gacaggaaac gatagtgatt ctcttgttga gtagcataaa aatacttaga
     8161 tactagtttt atataaataa catatagtga ttattggcgt aattacaaaa aacgagaata
     8221 acttaagtaa gcatttctaa ataattgaga aacctcatct aaaaagataa gaaaatatcc
     8281 ctaataaaac ttctgtaaca ttttagataa ctgattgtaa tctgaataat aaactataag
     8341 gagacggtta gacggtctcc tttttttgcg tattataatt ctagaattgt tggaaattga
     8401 taataattaa caagaaatat tagacaggaa cttcgaactg cttatcacat tcttattatt
     8461 atatatttgc gtaagtagtc ctggtagtct caatgcacta ttctatcttg aaactaagaa
     8521 gttatagaag gagcaatgat agaaagcacg agaatacaaa tagcgattaa atatttttct
     8581 agacaacctt agttaattca cacagatata ttttaggtac agtcttacag atatagagtt
     8641 agtgctgtgt gaatcctgat gttcgcagtg cataggagtc aagtaagatt ctagccagta
     8701 tagaaagtgg aagtctggag cgtacctcat aatctgggaa gaaggagcct tctgatttaa
     8761 agccaacaaa aggaatctcg atatgttcca tcaaacttga atgacgatta gcagtaagca
     8821 aaattgtccc gatctcagct ttataacagt tttcagctgc ggtagtcaat tcctttgttt
     8881 caccgtttag tgaaacaaag atcacgatgt catctttgga taattttcta ctaatgtttt
     8941 ttatgatatt gggatcagta tgcatctcac aatatttacc ggtaagttga aatttgacca
     9001 tcatttcctg agcaatcaat tctgaaaaac ctcgggcaaa aatcatgatt cttctagcag
     9061 ccttgattcg ttgaatagca tcttcaatat tcccgctttc taccatattg atggtgcgga
     9121 ttacctcttg ctcattttta agaatagcag tctttattcc ttcatcaact ttatcaagag
     9181 cggcaaaatt gatatttgta ttttcttttt cttttaacga atgtttaaaa gacgtaaacc
     9241 cctcatatcc ttttttcttc atagtacgaa cgacggttgc tgttgaaacg ttggcatatt
     9301 cacttagttt tacaatggaa taggagggga tttcgagtat gtgactttga ataaaatccc
     9361 agagatattt ctccgattca cttaattttg tatccatatt ttgcctccga ttagttagga
     9421 taattctatt gtaaagcggg agtttccttt acttcaagtt tatttaatat attttgtaaa
     9481 agttttcaat gaatttagtc atttatgaaa acatttacat tcgatcttga tacataatga
     9541 atatgtaaga aaggaggagc gtaaatgtca gtttatacat gtactttaaa cttggcaatc
     9601 gaccttttta tcgaaacaga tgaaatgaaa ccatcccaag tcaatcgaac aaacgatgat
     9661 gatatacaag ctaatggaaa aggtgtgaat gtttcattga tcatgaagca attaggtatc
     9721 gatagtacag ctttaggatt taaagcaggt tttaccggga attatattga ggattatttg
     9781 catcaaaaag atattagcac tgattttatt agcgtaccgg gaatgactag aatcaacgtt
     9841 tttacacaag ttaataaaac caatgaagaa ttcaaattgg ttaataaagg acctgagatt
     9901 cctcaggaac aaatcaaaac ttttctaaat caaatacaaa agctgcaagc tgacgattat
     9961 ttgtgtgttt ctggcagttt gccgcgaggg gtctcacccg aaattcttat tgatatcagt
    10021 cgtatttgtt ttgaaaataa ggtccatctg attattgatt caagctatcc ggaaataatt
    10081 gattgtctgc cgtatcagcc gttcttatta aaaccaaacg aggaggaatt gcaatcttgg
    10141 tataatgtcg agcttagtga tcgtgaagga tatattcatt atgctgaacg cttgattgcc
    10201 gatggagcga aaaatgttct cctttctcta ggaggagagg gaggaatatt tttaaataag
    10261 gatatcgttt attctgggaa ttctcctaaa ggtaaagtag tcaatacagc gtgtgctggt
    10321 gacactttgt tgggaacatt tcttgcagga tatttaaaag gtgaacaggt agatattaat
    10381 ttgaagaaaa gtttagcagc tggaagttca accgcttttc gtaaaggatt aacagatttt
    10441 tcggatgttg cagagttgga aaaacaaatc gaaatcaaag agggagcgag taaaaaatga
    10501 caacttacaa aattgttgcc gcaacaggat gtccaacagg aattgcccac acttatatgg
    10561 ctcaagaagc attggagcag gcagctaagc gcaaaggggt atctatcaaa gttgaaaccc
    10621 atggtcaagt agggatagag aatgaattaa caccagaaga aataaaaaac gctgacgctg
    10681 taatcattgc agctgacaaa gatgttcaag cagaacgttt cgcaggtaag cgtgtaatcg
    10741 atgtgcctgt cagcaagggg attaaagatg ctgaccaatt gatcgaagat gcgttggctg
    10801 gtaaaggttc aatcactgca gaaaacaaaa cagttgacgc tgctggtgag aaagcggaga
    10861 tgggggttgc agcaggcatt ggtcacagta tttataaaaa tctcatgaac ggtgtttctc
    10921 atatgctgcc gtttgtagtg gcaggtgggg taattattgc aatttcgttt gccatctggg
    10981 gtgtgtactc ttttgatccc aatgatccta cttacaatcc gactgccgct atgttaaagg
    11041 gtgttggtga tgcggcaatg ggaatgatgg ttcctatttt agcagcttat atcgctgaag
    11101 gaatcgctaa acgtcctggt ttggttgttg ggtttgttgg cggactgtta gccaattctg
    11161 gcggagccgg tttcttaggc ggaatttttg caggtttctt agcaggctac tttatcttac
    11221 tattgcaaaa ggtcatgaaa ggcttgccta aacaattgga tggtctgaag gcgatcttct
    11281 tatatccggt gatcggtgtc ttgtttgttg gtgtagtcat gtctttatta gcaaatccaa
    11341 tggaagcagt caaccaagga atgatgaatt tcttggcagg attccaaaat tctaatccat
    11401 taattttagg gattatcgtt ggctgtatgt cagcatttga tatgggaggt ccagtcaata
    11461 aagcggctta tgttacaggg actgcgttgt tggcacaagg taaccataca tttatggcag
    11521 gggtttcggc agcttgtatc gcgcccccat tgattactgg atttgccgca ctgttttttg
    11581 gaaaatattt cgacacgaat gatcgcaacg ctggtttagt caactttatc ttgggctcaa
    11641 cgcacattac cgaaggagca attccatttg cggctaaaga tccgctgcgt gttctgccaa
    11701 ctatgatgct aggttcatca attgctgctg tactgacata tatgtttggt gtccaagttc
    11761 ccgcacctca tggaggtttt ctagtattac ctgttgttac tggtaaggtt gcatgggtag
    11821 ggtcgatttt acttggttca atcgttggcg gagtaatttt agggttgatc caaaaacgta
    11881 gtgtagaaaa acgtgaagca gaagcagctg ctgttgaaat gaaagaagca accaacaact
    11941 aaaggttatt ctagaaagaa ggatttcaaa tgactttgat caaaacaaat aaagttttta
    12001 tgaataaaaa ttttacgacg caagatgagg taatgaactt ccttgcagat cagtcaattg
    12061 tcttgagcat tagtgaagac cgagatgcag tttacgaaaa attgatggaa agagaagaag
    12121 aaagcacgac tggtatgatg gatggttttg ccatccctca tgccaaagat gctagtatca
    12181 aggatgccga tattgtgatt gttcggttaa acggtaaggt cgattggaaa agtttagatg
    12241 gagaaggaac ggattttgta atcgctctgt ttattccaga ttcagaggct ggaacaaccc
    12301 atctaaaatt tttatctcag gtagccagac ttttgatgca caaagatgtg acagaaggtc
    12361 tgaaacaagc ccagtctgct gaagaaatag ctgaactttt aaaccaaaaa ttagaagaaa
    12421 actaaaaagg aagtagtgaa atcatgcctt taacagaaaa aaagaaaact tatattcgtc
    12481 aactaagtaa tgaaagtggc atcatcagtg cgttagcaat cgatcaacgt ggtgccttga
    12541 aaaaaatgat cggtaaattt caaaataaat cagcaaccga tgaacaaatc atcaatttca
    12601 aaaaaatcat ttcttctgaa ttgactccat atgcttcagc aatcttatta gatccagaat
    12661 atgggttgcc ggcagccgaa gttcgcgata aaaatgctgg tctgttgttg gcctatgaaa
    12721 aaactggtta cgatgcttca actccaggac gtttaccgga tattttatct atttggtctg
    12781 taaaacgtct aaaggagcaa ggggccgatg cttgtaagtt cttgttatac tacgatgtag
    12841 atgaatctga tgacatcaat gaccaaaaga aggcgtttat ggagcggatt ggttctgaat
    12901 gtgttgctga agatttgcca tttttcttag aattggtttc ttatgatgcc aacgatcctg
    12961 atacttcttc gaaggaatat gccttgaaaa aaccgcataa agtaaatgaa atgatgaagg
    13021 aattttctaa gcctcgctat ggagttgacg ttttgaaagt ggaagttcct gttaatatga
    13081 actatgtgga tggttacact aaaaatgaag ttgcttatac caaagaagaa gctgctgctt
    13141 atttcaaaga acaatccgag actaccaacc tgccatttat tttcttgagc gcaggagtaa
    13201 ctgctgaatt gttccaagaa accttgaaat ttgcaaaaga ggctggctct acattcaatg
    13261 gggtactctg tgggcgcgca acttgggcga atggtgtaga accattcgtt actaaaggtg
    13321 aatatgctgc caaagaatgg atgcagacac aaggacgcaa gaacatcgaa gagctgaata
    13381 atgttttgaa agaaacagca acagctatta aattgtaaga aagctttaac tataacctta
    13441 agtttgtaac ctaatgattt ttaataatca atttgagctt ttaaataaag aattggaagc
    13501 gtcccgacgc ttcctttttc gtttgttttt aaggcatgat aggttctatg ttgtcgttca
    13561 ataagcgcaa gaaggtcagc atttttttct tagagtatgc aaaattgctt ttctacccaa
    13621 tgatttaaaa cggagaggca cccttggata tggtagatgg tgaagtaacg catttcttct
    13681 gtgatcacgg tttttttgtc ttcttgtagc cagattttta aataattttc ttcgatcaga
    13741 ttatttaatc tcttttgaaa ttggatatct ccgtggttga tgacgaataa cttgtatacg
    13801 aacgtattcg tatagacata gtcgatcaat tgagcgtata gatcctcgta gctatgtgtc
    13861 gtatcgtagg aaatgatttc atccaagtct tggaaaaagc tattttccag tttttcataa
    13921 agatcataga tatccgtata atgtgtataa aatgtcgaac ggtgaagatc agcagtttcg
    13981 gtcagttcac ggatggtgat gttttgaatt tttttatcta ctaataattt tgataatgct
    14041 tctgctaacg ctttttctgt ttttttgacg cggcgatcct cttttttcat tttgcagcct
    14101 cctaatcaac ataatcaagg aattgtgtcg gttatccgac aatttcaccg ctatctgaac
    14161 atagataacc gacaccttgt ctattataat ataagtatag catatcaata tttaaagaat
    14221 aggagaataa gtgatggaat tagcctttaa gcaactggaa aagaaagact acaaaaaagc
    14281 cattcaattc gcgattaaag gtatgcactt tgattggtat accaagaaca aactcctttt
    14341 gaatctttat ggaagatact tttggtatct agaattgaat cgcgcgacac aaatcattgc
    14401 tttatatgcg gatgatgagt tggcaggtgt tttattagca gacatcaaag gaaaatcaaa
    14461 aaagcatcat tcatttagtc aaaagttata tgttaaacta tttgatttct tacaaaatgc
    14521 atttgtgaaa gacagcaaag gcgggtatga cgacaccaat gacgaattgc tttcagacta
    14581 tttaaaaaaa cattccccag atggagaaat tgtcttttta gctgccaacc ctgatttaaa
    14641 gatcaagggg atcggtagta aattattaaa agaatttgaa cgccgagagc aaggaaaaga
    14701 agtatttctg ttcacggata gcggctgtac gtatcaattt tatgaacgac gcggtttcga
    14761 acggaaaaaa gaaaaagata acgtcatgaa gatccttaat aaagaagtag atctccgctg
    14821 tatgttatat agtaagatca ttcagtaatg ttttttacta tcagaaagca gaagtaccac
    14881 gaaggtcaat ggaaaaagcg agtaattgat ccatttagat gaagataggt gtagtctcaa
    14941 agatattagc tggcaaagat atccccagtt aaaaatagat ttctgtatcc aacaataaaa
    15001 atatttttgt atttattagt atatatactt tacaaaaatt tgtatatata ctaatttttt
    15061 tgtaggatca atgaaatgag gggaaaatat gccaaaatat aaagaaatag ccttggctct
    15121 aaaagagagg atcgttgagg gaaaatatca ggaaggttct aaattacctg agcaagaaag
    15181 tctggctaga gagtttaaaa caagccgtgt tactatccgc aaagctatac aactccttat
    15241 tgatgaaggt ttgttatata cgagacgtgg atctggtact tttatacgaa gcaatatcag
    15301 acaggacaat gagaatgtca ctcaagtaaa taatgtattt ggcacttcct cacaagaaaa
    15361 tacaaatgtg acaagtaaaa taatccgttt tgatgtccgg tttccgacag attatgaaag
    15421 gcggctttct gcaacctact tccgatcccc ttcctgcaaa tcatgggtat aaaaaatagg
    15481 catattaagc gggctaggag gagaaatatt cacatatcat tttttcattc cgcaagctgc
    15541 gtcaagttat ttggaatttg tagaacagat gagagaggta gaagcagcac tacaaactac
    15601 ttttcaataa gtaggaatta tagaaatttt actcaaaaaa tcttcattaa aaaatctgac
    15661 taaataagtt gagaaaacag aatatacttc aatatccagt gatttataaa tatcattcac
    15721 tcatgatacc cagctccgta tcataagtga tgcggttttt atcattgatc ctaaaaatct
    15781 tagcaagaca aaaagtacgt aagaaagtaa agactaaata cttcggagga acaaatgaat
    15841 aacaatgata ggttattgcg attgcgttat gcgattgatt tgaaagatac ggatttgatt
    15901 aaagcttttg agctaggcgg tgtaccactg actaaagaag aagctcaggc ggtactgacc
    15961 aaagtacagg ataagaacaa agataatgca gaaaataatg tgtatgaaaa aaccatcaac
    16021 aaccaagttt ttgacgcatt cctaaatggc ctgattctct tggcgcgtgg tccgcaaaaa
    16081 gataagccga tagtagacac gccgcaaaaa agcaaggaaa tcaaatatat caacaatgtg
    16141 ttgctaaaaa aattaaaaat cgccttgtct atgaccactg atgatatttt agaagttttt
    16201 gctgaggcag aactctatcc atcaaaaggc gaaatcgggg catttttgag aaaagaaggc
    16261 caacgaaatt ttaagccttg cggagataaa tatatgcgta attttctcaa ggggttagga
    16321 atttataatc ggagaaaagt ataaagaaat ttcatgagaa atcaaaacaa tggttgtttt
    16381 gatgagagct agtttttgta tgagattaaa atattatctt aatcgaggag ctggattggt
    16441 tttatgaatg atgaatataa gaatgatgaa gataagatgc tttttgaaga gatagagaat
    16501 cgatgtaggc tcaattttga gcttcgggga aagatgtctt taatacaaca aaaaaagtat
    16561 ttagcgaata aatcagagtt tactctggga catgttgaaa aattaatctc ggattggatt
    16621 agtagtagaa gcgaatttac aaaaataaaa cagccgatta aatttgacat gaaaaaactt
    16681 ttgttaaata agagtgagat aggtaataga gatcaatata ttagagctaa agggcaagaa
    16741 ataattgatt cattaggtga agtgaggtca tacaattatt tgtatgtaac acatagagct
    16801 gatggaatgg tcattactgt ggggaaatct tcttctaatg atattttttt agatggagat
    16861 cttttttatc aactcaatac aaatcacttg tctggcactg aaaatataat tttaaggacg
    16921 gaatatggca atgaaatatt tgcaaaatat gatgagattt taaaaaatta tttggactgg
    16981 gcttggataa ttccggttga gtcaggagat gctaagaaac tggagagatt attgggcgat
    17041 gaactaatta ataaaaaagt accaatttta aattattatt cccatagaca ataaaaggct
    17101 atttttcatg ataatactag ttataactgt aaaaatggaa gaaaatctgg gaaagaagtt
    17161 tttaaataag tgtattgttc gattttaata aataataagc aataccatcc gtctgtgttg
    17221 cgcaacatcg tactacaatg aatatttttt cacaaaaaga aaatcatcca tcgaatgaat
    17281 ggaaggtttt agtatacacg aggttaatgt cccctctttt ataatcagag gagtgaggtt
    17341 ttttctatga atgtttctat tttcttattg ctagtctagt ttctcttacg atgaaattgg
    17401 gattctattc cagtttttat tgatttgctg ataaaataac ttgaatggtg gatataaaca
    17461 tgataaataa gagaagaata acaaaacaag gttgtgaaat gattctcaaa taagtataat
    17521 agaaagaaaa aggaggaaga atcaatgaaa atagcaattg caggcgctgg cgcaatggga
    17581 agtcgctttg ggttaatgct tcaccaaagt gggaacgaag tattattaat cgatggctgg
    17641 gcagaacatg tccaacaaat caaagaacat ggattacaag caaattttaa tggtaaagag
    17701 gtagaagcaa aactaccaat cgttcttcaa tccgaagtag aaaaagaaga tcaagttgat
    17761 ctgattattc tatttaccaa agcgatgcag ctggaaaaaa tgctgcagga tatccaatca
    17821 ttaatcaaaa aagatacaga ggtcttatgt ctattaaatg gtatcgggca tgaagatatt
    17881 attgagaaat ttgtaccaat ggaaaatatc tatattggaa ataccatgtg gacggcaggt
    17941 cttgaaggtc ctggtcaggt caaattattt ggaagtggtt cagtagaatt acaaaattta
    18001 ggtgatggaa aagaagcagc tgcaaaaaaa ttagcagaca aattgtctga atcaggatta
    18061 aatgctcatt tttctgacaa tattcactat tctatttatc gcaaagcatg tgtgaacgga
    18121 acaatgaacg gactatgcac aatcttagac gttaatatgg ctgagctagg aaaaacatcg
    18181 actgctcata aaatggtggc gacgattgtc aatgagtttg ccaaagtagc agcagtagag
    18241 aagattgaac tagatgtccc agaagtcatt gcacattgtg aatcttgttt tgacccggaa
    18301 acaattgggt tacattatcc ttcaatgtat caagacttga ttaagaacca tcgattaaca
    18361 gaaatcgatt atatcaatgg cgcaatttct agaaaaggga agaaatatgg tgttgcgacg
    18421 ccttattgtg atttcttgac tgagcttgtc catgcaaaag aagatagttt gaacgtaaaa
    18481 taaaggagag attgaatgga aacttcacaa aaaatatcac cgaaaatttt tctgaataaa
    18541 gtcctcgctg gtacagcaac aggaatcatt attggattga ttcctaatgc agtattaggt
    18601 gctattttaa aatattttag tgcgtatccc tttgcggtaa cgatcgcaca gttagcagtt
    18661 atttttcaat tagcaacacc tttgatcatt ggtggtttga ttgccttaca atttggattt
    18721 gatccgatga gaatgatggt tgtagccgga gcttcttttg ttggttctgg tgtagtcaaa
    18781 ttcacacctg atttgggtga aacgggtgtt tacgttggtg ctggtacagg cgatattatt
    18841 aatacgatgc tgacagcatc gattgctgtc gggctgattt tactgattgg aaatagtttt
    18901 gggtcagtgt cgattgtgtt aacaccgatt gttgtaggtg taggtgcagg tttacttggt
    18961 ttttatttat tcccttttgt aacagcaatt acttctgcaa ttggatcatt gattaataac
    19021 ttcactacct tacaaccgct gttaatgtct atcttgattg gttgttcctt tgcctttttg
    19081 attatctctc ctatttcaac agtagcaatc ggaatggcaa tccaactaga tggtgtttct
    19141 gctggtgcgg ctgcgatggg cgtagctgca accacttttg ttttagtagt gaactcttgg
    19201 aaggttaata agtcaggtgt gacaattgcg attgctttag gcgctatgaa gatgatgatg
    19261 cctaatctat ttagaaaacc tgtgattctt attccttgtt tagttagtgc agtgattaca
    19321 gctattcctg tcgctctatt ttctatttca ggtacgcctg cttctgctgg tttcggtgtg
    19381 gtcggacttg ttggtccgtt ggcttcttta gatgcagggc taaatggctt tcttgttgtt
    19441 ctttgttggt tagtcatccc tgtttttgcg gcactaatta ctcaattttt atgcgagaaa
    19501 gtatttaaac tgtatgatcg agaagaagtc ttcaaatttt taggctagta gattttacta
    19561 agaaattttc aatcaatgga tgcgtaagtg agatgtcaac aacatctgac ttacgttttt
    19621 ttgccaaaat atggttttta ttttttatta ttaggtgtga ttagtgattt atcgacaccg
    19681 tatatccttg gcctttttta tcccaaactg aatcaaatga ctaccgtaat tagtgtgttt
    19741 ggtgacgtag atagtccagt cagacgtgct ttcttggtct ggtcagttgt atctggactt
    19801 ttttttgttc tttcgttgcc tgcgttgtat cacctatttg ttggaacatc aaaaacactt
    19861 gcaatacttg ctgttgcaac agttggttta tatgggatag gtgattgtat ttttactggg
    19921 ttattcagta tcaatacgaa tgaaagctct tggaatctat cgacttggat ccataataca
    19981 ggttctgggc taggctatgc aggctttcta cttttcccat tgttacttgt tctgctttat
    20041 cgtcaaa
//
LOCUS       DACCCR010000047        19858 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCCR010000047 DACCCR010000000
VERSION     DACCCR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.054"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(55..399)
                     /locus_tag="IVK93_001734"
     CDS             complement(55..399)
                     /locus_tag="IVK93_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP6387022.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(536..1672)
                     /locus_tag="IVK93_001735"
     CDS             complement(536..1672)
                     /locus_tag="IVK93_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6387023.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSCQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLEQLVRAVKK"
     gene            complement(1861..2835)
                     /locus_tag="IVK93_001736"
     CDS             complement(1861..2835)
                     /locus_tag="IVK93_001736"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6387024.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(2915..3790)
                     /locus_tag="IVK93_001737"
     CDS             complement(2915..3790)
                     /locus_tag="IVK93_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP6387025.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     ALIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKEWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3920..5827
                     /locus_tag="IVK93_001738"
     CDS             3920..5827
                     /locus_tag="IVK93_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP6387026.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(6367..9681)
                     /locus_tag="IVK93_001739"
     CDS             complement(6367..9681)
                     /locus_tag="IVK93_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP6387027.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESCTYT
                     NAQEGFALMVRDAVPKDHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIKLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSFLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTVSKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNESPNESPNESPNESPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGG
                     ILLIGVVVYVMKQRKK"
     gene            complement(9953..10498)
                     /locus_tag="IVK93_001740"
     CDS             complement(9953..10498)
                     /locus_tag="IVK93_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6387028.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(10510..11202)
                     /locus_tag="IVK93_001741"
     CDS             complement(10510..11202)
                     /locus_tag="IVK93_001741"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP6387029.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(11221..11505)
                     /locus_tag="IVK93_001742"
     CDS             complement(11221..11505)
                     /locus_tag="IVK93_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387030.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(11518..12081)
                     /locus_tag="IVK93_001743"
     CDS             complement(11518..12081)
                     /locus_tag="IVK93_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6387031.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            12308..12958
                     /locus_tag="IVK93_001744"
     CDS             12308..12958
                     /locus_tag="IVK93_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP6387032.1"
                     /translation="MIKRNWPWLLALVLLFLFGFSFVLGLLLIGVVLVLWGLFGSRRK
                     KGKQEEIPALTNELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAKLKAI
                     DLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIKNKQT
                     YEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQKD"
     gene            13082..14278
                     /locus_tag="IVK93_001745"
     CDS             13082..14278
                     /locus_tag="IVK93_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP6387033.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(14379..15755)
                     /locus_tag="IVK93_001746"
     CDS             complement(14379..15755)
                     /locus_tag="IVK93_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6387034.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSFSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIISILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(15792..16658)
                     /locus_tag="IVK93_001747"
     CDS             complement(15792..16658)
                     /locus_tag="IVK93_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP6387035.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(16747..17772)
                     /locus_tag="IVK93_001748"
     CDS             complement(16747..17772)
                     /locus_tag="IVK93_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6387036.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYRLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(17790..18464)
                     /locus_tag="IVK93_001749"
     CDS             complement(17790..18464)
                     /locus_tag="IVK93_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6387037.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKP
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(18645..19541)
                     /locus_tag="IVK93_001750"
     CDS             complement(18645..19541)
                     /locus_tag="IVK93_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6387038.1"
                     /translation="MKKRTGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     RNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYWKFKRQKQLRKNNRS"
     gene            complement(19615..19740)
                     /locus_tag="IVK93_001751"
     CDS             complement(19615..19740)
                     /locus_tag="IVK93_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6387039.1"
                     /translation="MKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKFKMKSEV"
BASE COUNT         5799 a         4224 c         3556 g         6279 t
ORIGIN      
        1 tagtcatcta acaataggcc gtttcaaaac tagttgtctt tttatatcca cagatcattc
       61 gactttttcg aataacccac ggctaatcat cccatccatc agaaaataaa atttatcttg
      121 gctcattttc tgttttggat ctttgaaaat attgactgct ttcaatccat ttgctgtagt
      181 gatcgacatt ttcaatgttt gaatatcttt tgatacagtg atttttaatt tgaatccttc
      241 tttgctttgc ggcgtggcat ctaatggtcg gatcaattga taattgcctc cgtttgtttc
      301 tgtaaatcca ttatcgcgca atgtatatct ttttgcatca ggatgcaaac gatagctaat
      361 tggtgcgccc agtaccgttg ctgttgtttc aaatgccatg attttcacct cgataaaatt
      421 agtttctctt tattataaaa gtttatcctg atttttacta ggtttttcag ttaagttccc
      481 taaaaaatga aggcgttctc tcagacatct gttcatataa gaatgacttc actagttatt
      541 ttttgacagc acgaactaat tgttcaagga aatactggat gtcgtcttcg gtatttccca
      601 aaccaaagct gatacggatg gattctttta gtatcggtgt atcttctcca tacattgctt
      661 ctaatacgtg tgaaggctcg atattacctg ctgtacaagc tgaaccagta gaaatagcga
      721 ccccattaag atcaagcttc atcagtagca aatcatttgc ttcgccaatt aagcgaagat
      781 tcaacacatg agggagttta ttcgttaaat caccatttac ttgataaggg atttcgtttg
      841 cttctaatgt atcaagaatc aattgctgga atcctttata tttgttgtta cgtgcttcac
      901 gttcttcagg tgacagcagt tcaactgctt ttgccatacc ccagatccct gctaaatttt
      961 ctgtacctgc acgccgcttt tcttcctgct ccccgcctaa aagcaatgca gggatattta
     1021 ctccgtctcg tttatataag aaaccgacgc cttttggccc attgatttta tgagcagaga
     1081 tacttaataa atcgattcct aattcttctg gatggatcgt ttggtttccg tacgcttgta
     1141 ccgcatctgt atggaaataa gctggatgat ccgctaaaag ttcaccgatt tctttgattg
     1201 gcatcaaatt tccgatctca ttgttcccat acatcacaga gaccaagatc gtatcttctc
     1261 gtaacgcttt tttgacctct tcgatcgaca gattcccttt ttcatctaca ggcagatatg
     1321 taacgtcaaa cccctttgtc tctaaatatt tcatggtatt aagaacagca ggatgttcga
     1381 tagctgtagt gattagatgt ctgccttctt tttgacacga gaacgctgtt tctaagatag
     1441 ctgtattatc cccttctgtt cccccactgt taaagatgat ctcgtgaggt tttgcttgca
     1501 gactgtctgc aatgatttgt cttgctgttt ccaatttttc atgcgcgtgg cgaccaaatc
     1561 catgtatgct tgaaggattt ccaaacgtgt cttgcatgat tgccgtcatc gcttcaatta
     1621 cttgtggatg catgggggta gttgccgcat gatccagata aatagttttc acttcattca
     1681 tcccttccaa attattgcta ggcacattct agcataaaat agcgaacaaa ccaaagaaga
     1741 gctcattttt gtctagagga cgtttcatgg gcatagaaaa aaggagctat gacaaaagtc
     1801 ttgtcgcagc tccttgaagc tgctcacttg tgtcacaact tctttccctc ttttttttgt
     1861 ttattcgaat tctttttctt ccagacggaa taacggactc atcggtgtat tttcatggat
     1921 gcgtttgacc gcttctccca ttaattctga acatgtaacg attgttaagt tttctggatg
     1981 acgattttct tctgtgaaaa cagaatcagt gatacaaata tctttgattg gtgcattgtc
     2041 taaaatactt ttcgcatgat cggacaataa tccatgtgac gcacaggcat atacttcttt
     2101 tgcgccgttt tcttttaata catttgctgc tcgtgcaagc gttgctcctg tgttcaagat
     2161 atcatctacc atgatacatg tctttccttg aacatcaccg atcacataac caccctcagt
     2221 tgaagcatca tctgcatgat ctacgatggc taatgtgctg ttcaaatatt ctgaaagact
     2281 acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact acgatgtcat ctcccgtcag
     2341 acctaattga cggtaataat gagcaaacaa cggcatcgta aataaattat ctacaggaat
     2401 atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa gtcagtactc gagttgcccc
     2461 tgcttcttcg atcaagttag cgatcaattt cgcagtgatc ggttcatgag gtttcgccgt
     2521 gcggtcttgc ctagcatacc catagtaagg caatacgaca ttgatcgttt tcgcacttgc
     2581 tcgcttcatt gcatcgatca tgatcagtag ttccatatag tagtcattga caggtgcatt
     2641 cgttgattga acgatataca catgatcccc tcgaatactt tcttcgatat tgatggaaat
     2701 ctcgccatca ctaaattgct tcacgacgct tttacctaat tctgttccga acactttcgc
     2761 gattttttca gctaatggac ggttgccatt caaactaaaa atcttcaacg tgtcatcctg
     2821 atacttatta ctcatgtagt cctccccaaa acttctctat tcttcgatag tttaacatat
     2881 ttttccaaga ttttctgtta atttcagcaa attcttaagg aagtgaaact gttccgttac
     2941 tccaactggc aggaatgact ttatgataat cgccccagtt gtcgtgtact ttgtaataac
     3001 gttctcctgt ttcagtttca aaataagcgt acgctgtcac ccaatgattg ccatatgtac
     3061 tgccaagtac tttcaaaata ccaatcatca caggtttgcc ttcacgtata cgttttgtag
     3121 ctcgctgcca tgaacctaga agagttgctc gtgcacgctc atgattgcca ttcttcttta
     3181 aaaaactaga aatccctgta ctaacctgaa aaggaatcgt aggaagtcct agtggttgga
     3241 tctggctacg caatgccttc gtcaaaacca acgaatcagc cgtatttttc ttgcgaatct
     3301 catttggaag catccactcc ttacgatagt cttgataata ggcaaggaga actgctgccg
     3361 cgtaagtacc acaaagatag cctgaggggg tagcccattt cttatacctc aaaaaacgtt
     3421 cttctggcaa tcccacccag caatttgcga gttcctttcc tgaccttttt tcttcgatca
     3481 aagcgggaat atcttctttt ctgttgatcc atctagataa ctcgattggc tcttcattga
     3541 tcagcttcca ttttttacaa gaaagaccct cttgataagt atattttgct ggagaatatt
     3601 ctaccggatg tcctgattga tcgataatca cataactttg atcttcaaaa accagagcta
     3661 aaaaaagcaa tttacttttt cttgactgca gctgctgagt gctggctaat tttttaattt
     3721 ttttatctac tttgaaatat aaaggtttat ccactacttg attcagttgg gatcgcctcc
     3781 tttttttcat tataagtgaa ggattgaaaa aaacaaatca gtttgattgt tcgaattttc
     3841 aattttttat gttctatttg ttaattcctt cattttgtaa gataatgggc acacgaaaca
     3901 aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa caagatttct atgaagcagt
     3961 caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat aaaccagcta caggcggttt
     4021 ccaagattta gttgacggga tcgaccaact tttgatggga gaaacagacg atctgcttgc
     4081 ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc cttgcatatt atcaactggc
     4141 aaacgattat gaccgaagaa atgagttgca tgctcaacca cttcaaccta taatcgaaaa
     4201 agttgaatca ttgcagtctt tcgacgaatt gaatgagcaa tttccagaat ggctgttgga
     4261 tacattacca ttgccattta atctggatgt agatgcagat atgaagaatg cgcaaacgaa
     4321 cgccttcttt gcttatccgc cttctttatt ccttccagat aagacgtatt atgcatcaga
     4381 ccatcccaac ggtgcacaac taataggcgt tttctttgat atggctgttc agttattaga
     4441 gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg gaacaagcag tgcaatttga
     4501 taaattgatt gctcctcatg taaaaagtgc agaagaaaac gcagattaca gcaaaatgta
     4561 taatccgcaa tcttttgata catttattca atacacagat aaactggact tgaaaaaact
     4621 tgtcacgggt ttgattggga caactccaga taaagtgatc gtaactgatc cagtgtactt
     4681 cgatcatttg aatgacatcc tgacagaagc tcattttccg ttattcaaaa gctggatgat
     4741 cattaaaaca gtacgttctc ttagtggtta tctatcagaa gaattccgcc aagtcagcgg
     4801 aatctattct cgcgtattgt ctggaacaga cgaagcaatg cctcagaaaa aagcagcgta
     4861 ttaccttgcc tctggacaat tcgatcaagt agttggcgat tactacggaa aaaaatattt
     4921 tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa aaaatgatcg ccgtttataa
     4981 aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca aaagaaaaag ccgtcataaa
     5041 attaaataac ttaggtatcc aagtaggcta ccccgataag atcccagcat tattcgacca
     5101 gttcaaaaca gtacctgcag atcagggtgg tactttgctt tcaaatactc ttgttttcag
     5161 caaactcaca ttaaaagatc gtttcagcaa atggaacaaa ccagtggatc gttcagaatg
     5221 ggaaatgagt gctgacacgg tcaatgctta ttatcatcca tttcggaatg tgatcgtctt
     5281 tccggcagcg atccttcaag caccatttta cagtctagaa caatcaagct ctgctaatta
     5341 tggtggaatc ggagctgtta tcgctcatga gatttcccat gcatttgata ataacggcgc
     5401 tttatttgac gaatatggca acttgaacaa ctggtggaca gaagaagatc ttacacaatt
     5461 tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt ctgccttttg ctgacggaaa
     5521 agtaaacgga aaattaaccg tttcggaaaa tattgctgat gcaggcggtc taagctgtgc
     5581 attggaagca gcaaaaacgg aatcagatct ttctatcgag gaattctttg tcaattgggc
     5641 aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa cttttactgc aaatcgatgt
     5701 ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa aaccttgacg aattctatga
     5761 aacatttggt atcacggaac aagacgctat gtatctagcg ccagaaaaaa gagtgcgaat
     5821 ctggtaagag gttgtgccag aagtggtcag cttcaagaaa taagaaggaa ttggggaaaa
     5881 ttgcttctca aatttttgtg acatttcggc ttatttttcg aagaagttac ttctggaata
     5941 ccatgtatca gaggttgtga aaaagccgct tagtttcaag tagtaagaaa aattttgtga
     6001 aaatagcttt ccacattttt gcataaattt gacttactac tgagaaactg gcttttgaac
     6061 accgtttatc gagaggttgt gaaatcagcg tcctgacctg agtattagag aaaaaacgga
     6121 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
     6181 accgtttagt ggagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
     6241 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
     6301 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
     6361 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
     6421 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
     6481 atttttttca ctttcttttg ctgtcctgtc cggatgagaa ggttttccat ttggtgattc
     6541 atttggtgat tcatttggtg attcatttgg tgattcattt ggtgattcat ttcctggatt
     6601 ttctctttct ggtttgtcat ttcccggggg cacatcggca gtttctcgca cagtgatcgc
     6661 tttttgctgg gtaacagatt ctgtatgagc agaccatttt tccccatcaa atatttccga
     6721 acgataagtg acttctagcg tatattctcc ttttgaaggt gctgaaaaaa tagcggtatg
     6781 attagaaaat tgagcacttg actgtgtttc aacgatttgg atcttttctg ggaaataacg
     6841 catatcttca aagacaggcg ttccttgatt tggtgtgaaa gttgacacaa aaactgtaaa
     6901 aggcttcagt tcaatgatat ttccttctgc attctccgcc acaccatttg cttcttgtat
     6961 aataatcgga ttattttgtt catttggttt tgtctgattt ggttcttgta cgattgcata
     7021 ctctggatat tccgaagatg gcgaggtttc tccgtcaaaa atagcaccta cagataatgg
     7081 aagaccatcc actctttcct tttctgtttg acgattcccc ggtttaatct gcatcagtcc
     7141 atgaacatgg atacttccat tttcatagcg cgtaacaggc tgctgacctt ctttcgaaca
     7201 tgtatttaga gactcgatcc aatcttttga tacggtatgt ccaagagtat tttctgattg
     7261 tttctctcgt tcattccaat agtagttcaa cgagtggtag atttgatctt cgttttgatt
     7321 caatgggcgg atctgctctc tcatctcaag attctctgtt cggaaagata agataccaga
     7381 agcatggtag tctgtgtttc ctgctgaaga ggtataaaga gccacattat atgaaccgtt
     7441 attaaaactt gtactgctat tgacgataat atctgttccg ctattggaat cgattccttt
     7501 cgcaagattt tcgaaagaca aactattgat caattcatga gggcccgtta aaaaagaacc
     7561 gcccattttg aatccattac cgttaccttc tccctcaatt cccggtaccc agccgtttcg
     7621 ataagcaact gagtttttga tgattacttt tccgatcgaa cctgtttcat ttttagcaaa
     7681 aaggtcccaa ccgtcatctg cattatgata cgcgatacaa ccgtcaaata catttccttc
     7741 tcctattgtc agtttggccg caaatccatc ggcatcttca aatcctggat cggcattttc
     7801 aaaagatgta cagttcttga ctaagttatg cgcgggccat gcttcgaaag gatcatttga
     7861 gctaccgctg atttggattc ctgtatttcc attccgataa gcatgaatct cttcgatcaa
     7921 attatgattt ccagaaatct gtaatccttt ttgcatagct gcactatttg taatgtcaaa
     7981 ccctcgtaaa ccccagtagt gactgaacag tgtcatccca tttccagttc ctttaaaatc
     8041 aagtacagga cgcttgtttt ctggattttt ctctgcaatc aacttaatcg ggttttctct
     8101 tgtcccgtta acgcctttgg gaatcgtcaa tcctctttcg aaggaataag ttccaggcgc
     8161 catgacgatt gtctgcccag catatgcgta tctcaatgct tgtgtcagag ataatggatt
     8221 ttgtctgctt ccgtttcctg catctgaccc ttctggtgtt acataaactg tcgaatattt
     8281 aggtttacgg tagtccactg tatgaagaat ctcttttgtt tcataactag tcatctctac
     8341 gtattcttct ggcggatagt ttttatctgg cgtaaaactg aaagtaaatt cgttcgttcc
     8401 ttccatcagt tttgtctgat aatcaaattc ccgattcgca gtgatagcca agtcttttgc
     8461 gttcttaagc acctctcctg ttgctttatt tttaattgtc aataatccgt cagcatttgc
     8521 tctaaaggac atagtatgat cttggtttcc tgaatgatta ctagaggtca cagtgtaaat
     8581 cggatcaatc aagcgagtag gtctttcttg cccaggagga tctgtcgcag gatctgagta
     8641 agcaacttcg attccttcta ccttgatcgt catattcctt gctgcagcaa atcctgcata
     8701 aataaccgat tcatctgaag cgtataattt ttcccagtca tacatgatgt cagaagccat
     8761 ttcttctcct gtttcaggat cgtaataata tgcctctaaa ccagtattcg tttttttcat
     8821 ctctacgtcc agtttagtaa tcgagttcgt ggccggaaac agattcccat gaccgtttcc
     8881 gaatatatta taagaacctg tttctttttc caagaaccct gctgaagtct ctaaaggtgt
     8941 tgtttccact gagacactcc caggagctgg cgcagcttgc ggatcttcag atgatattcc
     9001 tgtgattgcc cgtgtcccca atccaagtcg catcgtatat ttgctgccgc tcgtgttgac
     9061 cacttcttgt ctatccgagt cccagacata ttcgatccga ctgcctagta tggcaaatga
     9121 atttgaataa aatgcctgcc caaacaaatg atcttttggt actgcgtctc ttaccattaa
     9181 agcaaatcct tcttgggcat tcgtataggt gcaagattct atttcgatcg tcgcccgcat
     9241 cctaaagttg atgtctttgg ggattgcttg ataataatag gtcattccat ctgatccgct
     9301 tgactggaat tttccgccgt tattcgtgga agagagccgc acgccgtctt ctaatggttc
     9361 aatggcatac ctactagcac ttgtgctttc tccgaaaaca acccctttcc actcttcatt
     9421 tgcttcttca gattgctttt ttgtcttttc attttctgat tctttcatca taggatcatt
     9481 tccttcttcc gtatccatat cttcactagt tcttggtact tcctccattg ttgacatccc
     9541 ttcaatgttc acgtgtgcat tttcttcgtt cacagactgt tgttcgttag cagcaacttc
     9601 aacgacagaa attaaaaccg gggtactgag cataaggaga ttcatcatta gcgcactgta
     9661 ttttatacgt ttttttaaca agtgtattcc tcccgcaaca aattagaaaa cgtttactta
     9721 aagtattacc taatttattt ttactgtcaa taaaaaacaa aattataata agtgtatttt
     9781 ataaaaagaa aaaacataac ttaagttgtt gataacaaag agatttatta acgtaaaaag
     9841 aataagattt ttttaaaata acgtataaag aagagcataa aacaaagtcg tttgttttat
     9901 gctcttcttt tattattttg tactttttat tttatcaaat cactttgttt ttttataaaa
     9961 tttctgcgcc tagtgtggat tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga
    10021 aacttgccac ggcatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg
    10081 ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc
    10141 gttctcgtaa acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta
    10201 tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca
    10261 attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa
    10321 aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa
    10381 acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca
    10441 gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct
    10501 tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc
    10561 tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat
    10621 tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc
    10681 ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg
    10741 cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc
    10801 ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat
    10861 tcctggtaat tgtcctggtt tgtagccaaa tcctgttgca tctgcatcaa aataagctac
    10921 tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc
    10981 tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc
    11041 taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga
    11101 aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat
    11161 ttttacttct tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt
    11221 ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga
    11281 tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt
    11341 gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg
    11401 ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg
    11461 tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta
    11521 ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat
    11581 caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac
    11641 ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat
    11701 atgaaggact tcattagcaa aacctggtga aagatacatc gaagtaagat ggctcagcga
    11761 cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg
    11821 tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt
    11881 tcgaaattgc ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccaacacc
    11941 gccaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc
    12001 tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc
    12061 aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt
    12121 tcatttttca atttttctca aaaactcctt attagtttac catagataaa ccttagattt
    12181 tctctaaata tagaaaatcc ttctttagtc cgatactctt tctagtagtc cgtatgctaa
    12241 aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat gacatggagg
    12301 gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc tatttctctt
    12361 tggtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
    12421 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctaacgaact
    12481 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
    12541 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
    12601 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcaaaagctt tattcaaaga
    12661 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
    12721 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
    12781 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
    12841 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
    12901 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
    12961 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
    13021 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
    13081 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
    13141 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
    13201 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
    13261 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
    13321 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
    13381 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
    13441 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
    13501 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
    13561 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
    13621 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
    13681 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
    13741 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
    13801 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
    13861 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
    13921 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
    13981 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
    14041 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
    14101 tgaacgtgga atcgtggata tcgagaccct tcaaactact caaaatgatt tgatcgaaac
    14161 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
    14221 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
    14281 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgttggg
    14341 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
    14401 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
    14461 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat ggaaatgatt
    14521 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
    14581 ctcaacttta gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
    14641 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
    14701 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
    14761 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatt
    14821 gttaccgtca gtagcgttaa aatggcccct acagcgatca ccgcgcccac tgttccatgt
    14881 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
    14941 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
    15001 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
    15061 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa
    15121 ccaaaaggca tgaatggctg ccaattatca ggtttaacga aaaatattcc tacaatgata
    15181 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
    15241 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
    15301 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
    15361 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
    15421 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
    15481 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
    15541 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaaatga cagtgctgga
    15601 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
    15661 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
    15721 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
    15781 gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa
    15841 aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa
    15901 atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa
    15961 ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa taatccacag
    16021 atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg
    16081 cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag
    16141 ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc
    16201 ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca
    16261 gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc
    16321 gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa
    16381 ccagtagaca atggtaaccc tacagaaaca cccatgtact tcattccatg gaactgtcca
    16441 ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg
    16501 attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt
    16561 cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc
    16621 catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac
    16681 atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac
    16741 gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt
    16801 cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt
    16861 tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt
    16921 aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt
    16981 aactgaaagg ctgactcctt gtgcttcctt ttctatgctc acaatgatgt ttccgtgatc
    17041 cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc
    17101 atcggtcaaa acagtatgat cctcaccaac aatctcgtag cgaatatttt tttggataaa
    17161 ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagcctat attcttggat
    17221 gaggtagtcc cgcgaaaaac tgtctagtct tgcataatac aaaacttgtt ctacgtattc
    17281 atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc
    17341 gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga
    17401 gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg
    17461 ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg ttcgtccacc
    17521 gctcaaatag ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct
    17581 tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc
    17641 taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat
    17701 cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt
    17761 aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt
    17821 gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg
    17881 actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt
    17941 tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt
    18001 ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta
    18061 atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt
    18121 aatgcattga tttttgcaat cagtacatcg atttggaaag gttttgtcac aaaatcatct
    18181 gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg
    18241 atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga
    18301 agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga
    18361 ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat
    18421 tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc
    18481 tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa
    18541 aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcttttgtct
    18601 tttcaataca gcttaaattt attatgttat tcttaacaat tggtctatga tctgttattt
    18661 ttccttagtt gtttttgtct tttaaatttc caataacacc aataaccgaa aagcgttagc
    18721 actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt
    18781 tctatctttg aagagacttc ttctgtaaag ggaactcgcc ttcctgtcac tagtaatcgg
    18841 tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctctg
    18901 attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg
    18961 agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc
    19021 gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct
    19081 cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca
    19141 ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat tgctccgatc
    19201 atatgttgtt cgtagtattc ttttcctgga tttcggacat ttctaaccgc ttgctcaaaa
    19261 ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc
    19321 ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt
    19381 tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga
    19441 tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga
    19501 ctgatttggg tttgaggtct attacctgtt ctctttttca ttttcttcac cttcttattt
    19561 aaaaaaactg cagcaatcgg ttttataaag gttgctgcag cttatttttg ttgcttatac
    19621 ttcagacttc attttaaatt tacggtacca gctgtacgct ccaatcatca aacttattcc
    19681 gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat
    19741 attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata
    19801 gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataa
//
LOCUS       DACCCR010000048        19485 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCCR010000048 DACCCR010000000
VERSION     DACCCR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.055"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..411
                     /locus_tag="IVK93_001752"
     CDS             <1..411
                     /locus_tag="IVK93_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP6387040.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="IVK93_001753"
     CDS             509..697
                     /locus_tag="IVK93_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6387041.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="IVK93_001754"
     CDS             complement(755..1108)
                     /locus_tag="IVK93_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387042.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="IVK93_001755"
     CDS             1639..2061
                     /locus_tag="IVK93_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6387043.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="IVK93_001756"
     CDS             2058..2288
                     /locus_tag="IVK93_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6387044.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="IVK93_001757"
     CDS             2786..2986
                     /locus_tag="IVK93_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6387045.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="IVK93_001758"
     CDS             3013..4206
                     /locus_tag="IVK93_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6387046.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IVK93_001759"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IVK93_001759"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6387047.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="IVK93_001760"
     CDS             complement(5898..6821)
                     /locus_tag="IVK93_001760"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP6387048.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="IVK93_001761"
     CDS             7017..7622
                     /locus_tag="IVK93_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6387049.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="IVK93_001762"
     CDS             7930..8946
                     /locus_tag="IVK93_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6387050.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="IVK93_001763"
     CDS             9087..10388
                     /locus_tag="IVK93_001763"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP6387051.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="IVK93_001764"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="IVK93_001764"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP6387052.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="IVK93_001765"
     CDS             11117..11515
                     /locus_tag="IVK93_001765"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP6387053.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="IVK93_001766"
     CDS             11581..12663
                     /locus_tag="IVK93_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6387054.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="IVK93_001767"
     CDS             12875..14437
                     /locus_tag="IVK93_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387055.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="IVK93_001768"
     CDS             14430..15563
                     /locus_tag="IVK93_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387056.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="IVK93_001769"
     CDS             15563..16519
                     /locus_tag="IVK93_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387057.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="IVK93_001770"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="IVK93_001770"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP6387058.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="IVK93_001771"
     CDS             17808..18626
                     /locus_tag="IVK93_001771"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6387059.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="IVK93_001772"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="IVK93_001772"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6387060.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6227 a         3514 c         4192 g         5552 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaa
//
LOCUS       DACCCR010000049        19452 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCCR010000049 DACCCR010000000
VERSION     DACCCR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.056"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            133..3660
                     /locus_tag="IVK93_001773"
     CDS             133..3660
                     /locus_tag="IVK93_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6387061.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IVK93_001774"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IVK93_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP6387062.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IVK93_001775"
     CDS             7448..7789
                     /locus_tag="IVK93_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387063.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IVK93_001776"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IVK93_001776"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP6387064.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IVK93_001777"
     CDS             9146..11566
                     /locus_tag="IVK93_001777"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP6387065.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVK93_001778"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVK93_001778"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP6387066.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IVK93_001779"
     CDS             13000..13737
                     /locus_tag="IVK93_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6387067.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IVK93_001780"
     CDS             13751..14566
                     /locus_tag="IVK93_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6387068.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IVK93_001781"
     CDS             14579..15226
                     /locus_tag="IVK93_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6387069.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IVK93_001782"
     CDS             15242..15898
                     /locus_tag="IVK93_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6387070.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IVK93_001783"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IVK93_001783"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP6387071.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IVK93_001784"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IVK93_001784"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP6387072.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IVK93_001785"
     CDS             18240..18758
                     /locus_tag="IVK93_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP6387073.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IVK93_001786"
     CDS             18865..19365
                     /locus_tag="IVK93_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6387074.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3340 c         4177 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga ca
//
LOCUS       DACCCR010000050        19314 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCCR010000050 DACCCR010000000
VERSION     DACCCR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.057"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(59..2743)
                     /locus_tag="IVK93_001787"
     CDS             complement(59..2743)
                     /locus_tag="IVK93_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387075.1"
                     /translation="MKIIGKKIANRKINWLLISFLILIAMIIFTSNVAEGSIFNWFDD
                     TKSQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIVGYKMIIGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDSTTITDSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLNAGEGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDIANDTKNKFKEGLDDGTVSAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNTNHASAIQQKVKQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEIMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQDRRSVDVRENLQNTTSSQSGNRIVDVRENVQSSASNQSRTVDV
                     RENLQNTTSSQSGNRTVNVRENVLSSGTSQPRTVDVRENLQNTTSSQSGNRTVDVREN
                     VQSSTSSQGFKTVDVRENVQKDSGEMKEKAQKINIVEDRKSAKKFDSNHETVIIDSEI
                     RENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLKK"
     gene            complement(2968..3846)
                     /locus_tag="IVK93_001788"
     CDS             complement(2968..3846)
                     /locus_tag="IVK93_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HAP6387076.1"
                     /translation="MKSMAEKRMLSKTFLNNASFMKMSDSAKLLYVYLNTNADDDGIV
                     EAYPYVKMLGTPDDDVKILFTKGFVFFLNEDLLAFLPHWLQNQNIRIDRYKPSIYRFK
                     LLNLKPEFFSKLKPIKLDVILKSGQVEPLRVDLMEKWYPVGIPSINHLEVQSSQDQIS
                     SDKSSLVKNSIDKINQVDTIINEEIINEKGIQLIHKLGDRFTKSQREKFVQLIVDMKN
                     STEREFKIRIDGPHYYEQTNKMIIRLFNTINEKLNLGKELNEMKYVNTVSRNHWKEIA
                     LEEQDNRMYIIDALSK"
     gene            complement(3991..4293)
                     /locus_tag="IVK93_001789"
     CDS             complement(3991..4293)
                     /locus_tag="IVK93_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6387077.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGESFERK"
     gene            4495..5292
                     /locus_tag="IVK93_001790"
     CDS             4495..5292
                     /locus_tag="IVK93_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387078.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            5343..6485
                     /locus_tag="IVK93_001791"
     CDS             5343..6485
                     /locus_tag="IVK93_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6387079.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKMYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(6583..7974)
                     /gene="sufB"
                     /locus_tag="IVK93_001792"
     CDS             complement(6583..7974)
                     /gene="sufB"
                     /locus_tag="IVK93_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP6387080.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(7979..8449)
                     /locus_tag="IVK93_001793"
     CDS             complement(7979..8449)
                     /locus_tag="IVK93_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP6387081.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(8436..9671)
                     /locus_tag="IVK93_001794"
     CDS             complement(8436..9671)
                     /locus_tag="IVK93_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6387082.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHMTIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VIAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFKHGTI"
     gene            complement(9668..10954)
                     /gene="sufD"
                     /locus_tag="IVK93_001795"
     CDS             complement(9668..10954)
                     /gene="sufD"
                     /locus_tag="IVK93_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP6387083.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(10967..11740)
                     /gene="sufC"
                     /locus_tag="IVK93_001796"
     CDS             complement(10967..11740)
                     /gene="sufC"
                     /locus_tag="IVK93_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP6387084.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(11937..12785)
                     /locus_tag="IVK93_001797"
     CDS             complement(11937..12785)
                     /locus_tag="IVK93_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6387085.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(12809..13495)
                     /locus_tag="IVK93_001798"
     CDS             complement(12809..13495)
                     /locus_tag="IVK93_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387086.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(13488..14522)
                     /locus_tag="IVK93_001799"
     CDS             complement(13488..14522)
                     /locus_tag="IVK93_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6387087.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(14917..15255)
                     /locus_tag="IVK93_001800"
     CDS             complement(14917..15255)
                     /locus_tag="IVK93_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP6387088.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(15257..15613)
                     /locus_tag="IVK93_001801"
     CDS             complement(15257..15613)
                     /locus_tag="IVK93_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP6387089.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(15619..16806)
                     /locus_tag="IVK93_001802"
     CDS             complement(15619..16806)
                     /locus_tag="IVK93_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6387090.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYFYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVVGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(16997..17842)
                     /locus_tag="IVK93_001803"
     CDS             complement(16997..17842)
                     /locus_tag="IVK93_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6387091.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YPLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18076..19041
                     /locus_tag="IVK93_001804"
     CDS             18076..19041
                     /locus_tag="IVK93_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP6387092.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         5486 a         4045 c         3297 g         6486 t
ORIGIN      
        1 agttctaaga aatcatgcgt actaatcttt agaattaatt gcatgacaat cccccctttt
       61 atttttttaa tagattatcg aaaagtggat taggatcatt aagtgtatct ctgaataact
      121 cattgtcttc ataagtgaat cttcgtcttg attttgatcc cttatcattt tcacggattt
      181 cactatcaat aatcactgtt tcatgatttg aatcaaattt tttcgctgac tttctgtctt
      241 caacaatatt gattttttgt gctttttctt tcatttctcc actatctttt tgaacgttct
      301 cccgaacatc tactgttttg aatccttgac tacttgtaga actttgtaca ttttctcgta
      361 cgtctactgt tcgatttcca ctttgactac ttgtagtatt ttgcaagttt tctcgaacat
      421 ctactgttct cggttgactt gtcccactac ttagtacatt ttctcgtaca tttactgttc
      481 ggtttccact ttgactactt gtagtattct gcaagttttc tcgaacatct accgttctcg
      541 attgatttga cgcactactt tgtacatttt ctcgtacatc tactattcga tttccacttt
      601 gactacttgt agtattctgt aagttttctc gaacatctac tgagcgtcta tcttgactac
      661 ttgtagaatt ttgtagattt tctttgacca atttttctaa tccggtattt gaatttttag
      721 cttcttgaat tctttctttg gtcaacgctt ctttttcgtc catattagcc cctgtacgcc
      781 cttctacacg tttcctcaac atttcctcac gattcattat ttcaggcgtg tattttgctt
      841 cctgtcgctt ctgattaatt aattcttcag cagacttagc ttgaccgtgc atagcatttt
      901 tacgttcttc aatatcttgt ttaacttttt gctgaattgc acttgcatga ttagtatttg
      961 attcagcatt atttttcatt gcttcacgca tacgttcaga actagacata attctatcag
     1021 cgttttcaac tttagcgcca tcttttctat ccgaaagacg cgttggcata gaatcagcat
     1081 attttccagt ttcatcttct ttatttttag ctactatagg tttgctattt ttattaatag
     1141 cggacacggt accatcatct aaaccttctt tgaatttatt cttagtatca ttagctatat
     1201 cacgcatact cttaaatggt tttgttgctc tttcactagc cattgtagct ccatccgaag
     1261 ctaagcctga taatccacgt tcacgagcat acccaagcgt tttccccgtt ttattagctt
     1321 tttgagaaag tgattgacca aagttgcgtt caggttttcg ttcttcccct tccgcttgat
     1381 cgtttccacc actatttttc ccacctttag atcggttcat accacctgtt cctttacgaa
     1441 acagcatagc tcccatacca aatgctgaag caagctggcc atagccttct tttaaaccaa
     1501 tatccacgcc aaagtaacgc atgactgttc cactaccctt tattagaacg aacacggcac
     1561 agatataagc aattgttttt ataaaaatat tcgttgagat accttcacca gcgtttagat
     1621 aattaacaaa cattgcaaac atagacaatc cgaatccctg aaaaccgaca gtcaaataac
     1681 actgtagaat atctgacaaa acttgttttg atcgttgtcc tgtttctaag tcagtagcaa
     1741 ataccaaaac tccaagtacg cgtttaaatg ctaactcgag tattgctgta attataatga
     1801 aagcactaaa aacatacgct actgctaaag caatcaatcc aaatgaaatt ccccacaagt
     1861 tcgcttgata gcgataatat cctgatttga taccatcaac aaaggttgac aaaaaattat
     1921 ctgaaaattt agtagcaaca aattcattat cagaattttc aactaattca taacgcagat
     1981 tatcagctat tgggttctct ttttccattt tgtcgagtac attatctgtc aatacttgcg
     2041 taagatccgt tttccaaaaa ttttcttttg ttagattgtt ccggtcattc gttttatcga
     2101 tcatggaata ttgatccgtt ttattgatat aagctaaatc agtaacccct tgttcaatca
     2161 gcgaccacgc cacgccttcg tctgaatctg taatcgtagt tgaatcatta taaaaggttt
     2221 tagaaaactc tattccgctg tgaatcaaag ttggcattaa aagaatcaat gaaattgaca
     2281 ttacaacatt cattccaaca gattttagat caaccgttcc tttacctata atcattttat
     2341 aaccaacaat cactagtgaa agtatcatta agccaacaac aatggtactt aaaactgatt
     2401 gatagacttt gttcaatcca gcactttcca caaagctaaa caaatctaaa acctcgggaa
     2461 tcatattttg aatgccatcg acaacagcga acattgattt tataacagac cagcctatcc
     2521 acataccgaa ctgactgtaa aaatttccgt tatatttttt aagataattc tgataaagtg
     2581 gattagttaa aaactcagtt tggctttttg tatcatcaaa ccagttgaaa atactgcctt
     2641 ctgcaacgtt actcgtaaaa ataatcatag ctatgagaat aagaaaacta atcaacaacc
     2701 aatttatttt tctattggca atcttttttc caataatttt cattgttacc tccctcagcc
     2761 cagtttctga ggctctcaga gagcttttga gcaatttttt ggtcaatgat gatatgagtt
     2821 acataactat cattcactgc tcttaaatcg agaacaatag tgttgaagca gatacactcg
     2881 attaattcaa gtgtatctgc gttttggtca agacagcaga tttgatctat tagacgattt
     2941 ccaatcatat taagagtcct ttcttgttta tttacttaga gcatctatga tatacattct
     3001 gttatcttgt tcttctaaag caatttcttt ccagtgattt cgagataccg tgtttacata
     3061 cttcatttca ttcaattctt tacccaagtt taatttctcg ttaatcgtat taaataatct
     3121 gattatcatc ttatttgttt gttcatagta atgtggccca tcgattcgaa ttttgaactc
     3181 tctttctgtt gaatttttca tatctactat taattgaacg aatttttctc tttgactttt
     3241 agtaaatctg tcacctagtt tatgaatcag ctgtattcct ttctcgttaa ttatctcttc
     3301 attgataata gtatcaactt gattaatctt atctatacta ttcttaacta aactagattt
     3361 atctgaacta atctgatctt gacttgactg aacctccaaa tggttgatgc taggtatacc
     3421 aactggatac catttttcca ttaaatcgac tcttagcggt tctacttggc cagatttcaa
     3481 aatcacatca agttttattg gctttaattt tgagaaaaat tctggtttta agttcaataa
     3541 cttgaagcga taaatagatg gtttatacct atctattcgt atattttggt tttgtaacca
     3601 atgcggtagg aatgctaaca aatcttcatt taaaaaaaat acaaaacctt ttgtaaaaag
     3661 tatctttaca tcatcgtcag gagtcccgag cattttcacg tatggatatg cttcaacaat
     3721 tccgtcatcg tctgcatttg tgttcagata tacatataat aattttgctg aatctgacat
     3781 cttcataaaa cttgcattat ttaaaaatgt tttggacagc attcgttttt ctgccatgga
     3841 tttcacactt ctttctattt gaatggtaaa ataaattcaa ataaagtttt atcagaactt
     3901 tattgttggg tgtttccctt cgtctacaaa ctatcgggag cactctttta tatatttttt
     3961 tcaattcttt tcaagtctaa atttgttatt tcattttctc tcaaaacttt cacctctttt
     4021 cattcgagca aacataaact cattaactgc ctttttcgaa taaaattttc ttgcccctaa
     4081 ttcaacaacg ggcaatccct catttctcac cgcatttttt aaaaagtttt cgctcacatt
     4141 taagtattct gccatttctt tttgatttag atattctcta gtcatacccg cattttcttg
     4201 agccttctca atcgcttttg tagcaatttg ataaaactgc tttagaaaca tttcctcctg
     4261 ttcttctgaa atcattaatt ttattgcttc cacaaaaacc tctccttttt aattaattat
     4321 tctataactc ctctaccttt ataagtacaa ataaatacaa attgcgtaaa aaaatgtatt
     4381 tatttgtatt tataagaata aagtatcata ctccagataa aaagtaaata cttttaaata
     4441 cttttatttg ataaatatga tatactatct ttaaaaataa aaaggagaaa aaagatgaag
     4501 ccaaatcctt cagaagcagg aaaacgaatc aaacaattac gcctttcatg tggttttact
     4561 atggaagaac ttggaaggaa aatagataat tcaccacgtg cgaccatatc caactgggaa
     4621 cgtgggacca atttaccaaa tccacaaaag ctgaaattat tatcaactat cactaattca
     4681 acaattgact ggattaaatg gggaacttta gaagaatata taacttcata ccttatcgat
     4741 atagggtatg agctatatat taaggacttt ccagaaatac ctcacaaagt ctttaaagac
     4801 attcaagaaa gatatagtga tacgttttcg ctagataaag actatgagtt attgaatcct
     4861 ataataaaaa atatatttac aaaatattac tcaaaagatt ttgaaattta tctaaatgag
     4921 atacttgcta ataatatcaa tcctctgatt gaagagtacg ctaaccaaag acactctatc
     4981 tctaaagagc tatttttcaa aagagccaac agtaaacttc aagacaagat aagaaatggt
     5041 gatttcaaat acggtgaaaa agaaagaatg attgattctt taaaacagct attagacgaa
     5101 atggacttag cctataaaga tatttcaaag tattcatctg tcgaggattt cttttataaa
     5161 attactaaaa accaatttga aacagaaaaa tttatttcag atcttagtaa aaaatacaat
     5221 ttcccatatg agaaaaatag tataatcgca aaatttttaa ctgataatca cgataaattt
     5281 aaaaataatt gattcttcaa ataacttctc tacctgcaat aatgaagtaa ggagagaatc
     5341 aaatggcaac attttttagt tataaattac aaaatggcga aaaatattgg ggatttgaaa
     5401 cttatttagg gaaagatccc aagactggaa aagatatacg tacaaagcgt agaagattca
     5461 aatcaaaagc tgaggcgcaa acagcactta atagactact tgtagacttt aaagaaaaac
     5521 aagagtttaa aatcaatgtt acaacttttc aagagttgta tgaattatgg attgataact
     5581 acagaacaag agtaaaaccg tcttctgttg ccgttgctca acgtttttgc aaaaaccata
     5641 tattacctgc ttttggggaa ttacgattgg ataaaataag tgttagctat tgccaaaaac
     5701 aggtgaatga atggcacaaa acattaaaac aatatggttt cttgagaaaa acaactgctc
     5761 aaatttttaa atttggagta gcaatggaag tctgtaagga taatcctatg tctaaaacat
     5821 tgcttccaag aaaaattgaa gaagaacaac ccttgaagtt ttacacgaaa gatcaattac
     5881 aactgttttt acagaatact aaagaaaata attcagttaa aatgtacact tttttccgtt
     5941 tattagctta tacaggaatg cgtaaaagcg aggcattggc gcttcaatgg gaagatatag
     6001 actatttcaa caaaacaata acaattggta aaactatcgc tcaagacgag tttaatcaag
     6061 tagttttaca agtacctaaa actaaaaatt catcaagaac gattcagctt gatgatttta
     6121 cacttaaaca actgcgtatt tggcaacaag aacaaatgaa aataatgatc ttatatggct
     6181 ataacacgaa ttcaccaaaa cagtttcttt tcactacaaa cacaaacaag ttgtactacc
     6241 cccaagtagt aaacgattgg ttagactgga tttacaaaaa gacaccgatg gagcctcaaa
     6301 ttactccaca tggatttagg catacccact gtagcttatt gtttgaaagt ggtgcgtcga
     6361 taaaagaagt acaggaacga ttaggtcata aagatattaa aactaccatg aatatctatg
     6421 ctcatgtcac accacaatct gttaagaaaa ctggtgaccg atttgcaaaa tttatgggcg
     6481 aataattcca aacgtagcca aaaacgtagc caaccattaa aatacaaata aaaaaacgct
     6541 cgaaacgctt taatatcaac gtttcgagca atgaaatagt tgttaaccca cagacccttc
     6601 catctcataa ctgatcaatc ggttgagttc gactgcatat tccattggaa gttctttggt
     6661 gaatggttca acaaagccca tgacgatcat ttcagttgct tcagattcgg tcaggcctcg
     6721 gctcatcaga tagtagagtt gttcttctga gatttttgag actttggctt cgtgttctaa
     6781 agagacttgg ctgttgtgga tctcattgaa tgggatcgta tcggatttcg accattcgtc
     6841 catgatgatc gtgtcacatt cgatatgaga gatcgaaccc gcactgtttt tggcaaaggt
     6901 tacttgtcct cgatagttta cttctccgcc gtcttttgaa attgatttag agacgataga
     6961 gctagatgta tttggtgcat tatggatcat ttttgcaccc gtatcttgga tttggtttgc
     7021 tcctgcaaag gcgatggaga gcatggtgcc tcgtgcacct tttccatcta agtaaacact
     7081 tgggtatttc atcgttgttt ttgcacctaa gtttccgtcg atccattcaa cggttgcgcc
     7141 ttcgtatgct tttgctcgtt tggttactaa gttgtacaca ttgtctgacc agttttggat
     7201 cgtggtgtaa cggcaatacg catcttttct tgtaaagatt tctacgatgg cagcatgcag
     7261 actattactt gaataagtag gtgccgtaca accttcgaca tagtggatgc ttgctccttc
     7321 gtcaacaatg atcagcgtac gttcgaattg ccccgtgttt tccgcattga tccggaagta
     7381 cgtttgtaat ggaacatcga ctttgacgcc ttttggcacg taaataaagg tgccacctga
     7441 ccatacggct gaattcagcg ctgctaattt gttgtcggtt ggcggtacaa gttttgaaaa
     7501 gtactctttg aataattcag ggtattcttt caaagcagag tcagtgtcag taaagacgat
     7561 tcctaacttt tcgaattcat ctttcatatt atggtacacg acttctgatt catattgtgc
     7621 ggaagcaccg gctagataag ctcgttcagc ttctggtata ccaatctttt caaatgtatc
     7681 tttgattttt tctggtacgt cttcccagtc acgagcgggt ttgtcacttg gtttttggta
     7741 atatttgatg gcatcgaaat cgatgtctga aaggtcgggc ccccatgttt gcattggcat
     7801 tttattgaat gtttctagag atttcagacg gaattcaagc atccattctg gttcgccttt
     7861 gattcttgac atctcacgga tcacttcttc tgtcaagcct tctcctgtac tgaaaaccgg
     7921 ttcgacatca tcgtgaaacc cgaatttata ttcttctaat tctggtacgc ccatgttctc
     7981 actcctcttc cttgtggtaa tgatggaaag ctgcggtacc atcgttttcc attgcttttc
     8041 ctagtgcttt ccaggcaagt gtcgcacatt tgatccgagc gggaaattta gctacaccac
     8101 caagcatagc agcatcgcct aattggtcgg gatctttgac ttcttttcct tggacaagct
     8161 gtagaaagtc tacagttaaa tgttctgctt cttctattga ttttccaatg acggcatctg
     8221 tcatcatcga ggcacttgct gtactgatgg aacagccgct gccgtcaaag gcgatatttt
     8281 tgatgattcc atcttttaca tcaacttgca attcgatcac atctccacat gtggggttgt
     8341 tcaattcgat cttgcttgtc gagtcagtca agcttccacg atgatgtgga tgggaagaat
     8401 gatccaagat cacttgacgg tataaattgt ctaatttaga tagtcccatg tttgaaaaac
     8461 tcctttgttg ccatgattgc ttcaactaaa cggtcggcgt cttgcgttgt gttgtagaaa
     8521 taaaagctgg cgcgagcagt cgctgatact tcaaggtact tcaatagcgg ttgcgcacaa
     8581 tgatgacctg ctctaacggc taccccttcc atgtctaaag ctgtcgcgac atcatgggga
     8641 tgtaacccat ccaaattgaa cgcaatgact cctgtccgaa aattcgggtc ttgcggacca
     8701 taaacagtca atccatcgat tgcttgtaat tttggcaaca cataagcggc tagttcttct
     8761 tcatatcgat ggatattgtc catgccgagt tcttgcaaat aatcgattgc atgacctaat
     8821 gcgatagcgc cagcgatatt tggtgtccct gcttcgaatt tccaaggtag ttctttccat
     8881 gtactgtcgt ataattcgac aaaatcgatc atttcgccac caaattcaac aggttccatt
     8941 tcttctaacc attttcgttt accatataaa acaccgatac ctgttggtcc gcacattttg
     9001 tgtccgctga aagcaaagaa atcacagtct agtgcttgaa catcaatggt catgtgcggg
     9061 gcagattgag caccatctac tacgatgacc ccgccgtttt catggacaag ttcagctaat
     9121 tcttgaacag gattgatgac acctaagaca ttggatacat gagcaatgga gacgattttg
     9181 gttttgtccg taatcttttt cgctgcatcc tccatatcca ggaagccttc ttctgttagt
     9241 tcgatatatt tcaagacagc acctgttcgt tttgccaact gctgccacgg gataacatta
     9301 gaatgatgtt ccatgtaaga gagcactatc tcgtctcctg ctttgatata gcggtcgccg
     9361 aagcttctgg ctacccaatt caagcttgtc gtcgtaccac gagtaaacag cacttctgct
     9421 gtttcttttg catggatgaa cgcccgtact ttttctcttg cttgttcata atcatgggtc
     9481 gcgcgttctg ctaacgtatg gacaccgcga tgaacattgg cgttatcgtg ttcgtaatag
     9541 tgcgctaatg cctggagtac ttgttttggc ttttgggtcg tcgctgcatt atccagataa
     9601 actaaaggtt catcattgac gtcttgaaat aagataggaa aatcattgcg tagcttttta
     9661 gcatcgatca tccattcaac ttcccttcga ttacttcgac taattctttc cgaacttgtt
     9721 ctacagggat tgctgttaag acagaaccga ggaatcctcg aatcactaag cgctctgctt
     9781 cttctttatg caatcctcga ctcatcagat agtacatttc ttctggatca actcgtccta
     9841 cactggcagc atgtccagca gttacttcgt tttcatcgat caaaagaata ggattcgcat
     9901 caccacgtgc tttatcagaa agcatcaaga cacgactttc ttgttgggca tctgcacctt
     9961 ttgccccttt caagatgtgg ccgataccat taaaggtcaa tgttcctttt tctcggatca
    10021 ctccgtgttg caaaatatgc ccgatcgtat gaggggcttt attggttact cgcgtatcga
    10081 ttccttggat ttgtctgccc gagctgattg ccacgatttt gacttctgcg tgtgcacctt
    10141 ctcctgctaa atctgagtca aagtcagcga tcacatgacc gtcattcatg acaccaattg
    10201 cccagtcgac agaagcatct cgtaaaatgt gcccacggcg attcatataa gaagtaatat
    10261 tttctcctaa ttgatctacg gctgaatatt tgatttttga accagcttta gcgatgactt
    10321 ccacgatgat attacctgat gattttgcta cttgttcttt ggttgtctgg aaacgttcca
    10381 gataagaaaa ttcactatgt tcatctgcca cgatcaacac atgcttgaag aagtgctcat
    10441 cggatgcgcc atcttgaatg aataaggact cgagtggttc atcgatcacg acatttttag
    10501 gcacatacag gaaaataccg ctgttcataa aagctacatg gagtgcagtc aattggtctt
    10561 cgttcatgtt tactgctttt ttcatataat attcttggac tagttctgga tattcgatca
    10621 ttgcagtgaa caagtcggta aaaatgactc ctttttctgc taattccaca ggcagttgtt
    10681 caaaaatcgt ccatgaacct tgttggacaa gcacaggatt gtctttcatt gcatcaaatg
    10741 ccggaatcgt gcctgtttca ctgatggttt gtgtttcttt cacatcaaat aacgaccagc
    10801 gatggaattt cacccgatcg atatgcggca atggcagttc atctgctttc gctagtgcgt
    10861 cttgacggaa ggttctcatc caatcaggtt cgcctttact taaagaaaaa tcatttaccg
    10921 cgtcaagata atcgagccag tttttttctt tcattcttgc tcccccttac gcttcttctt
    10981 ctttatagtc gattcctaat tctttactga taccagcgta gccttctgct tccagacgtt
    11041 tagctaggtc agcattccct gtcatgacta ctcggccttc catcatgatg tgaacgacat
    11101 caggtgtgat atagttcaat aatcgttgat agtgggtgat gatcaatgca ccgaaattgt
    11161 ctccgcgcat ttcatttact cctttagcaa cgactttcaa agcatcgata tccagacctg
    11221 aatcgatttc gtctaaaatt gcaaatgttg gttctaacat caacaattgc aggatctcat
    11281 tccgtttctt ttctccgcca gaaaaacctt cgttcagata acgttcagcc atttcttctg
    11341 gcatatttag taaagccatt ttttcatcta actttttcaa aaatgccata acagaaattt
    11401 tgtcgttttc atcacgtttc gcattgatcg ctgcacgcat gaattccgca ttcgtgatac
    11461 ctggtatctc acttggatat tgcatagcta aaaataagcc aagtcgcgca cgttcatcta
    11521 cttccaagtc taaaacattt ttcccgtcaa acaaaatttc gccttctgtt acttcatagt
    11581 ttggattgcc catgatagcg gcagataagg tagattttcc tgtgccgtta ggtcccatga
    11641 tggcatggat ttctccggtt ttcattgtta ggtttacgcc tttaaggatt tctttttcct
    11701 cgatcgacac atgtagattt tttatttcta agacagacat gcattatacc cccaaattct
    11761 ctttcgttct cactcatttt agactaattg agaatgaagc gcaatgacct tccttactaa
    11821 aatcaaatat cttttattta ttattaaaca attttgattt ctaaattaga ataggtataa
    11881 aaaagatgag aaataagcca ttacagactt atttctcatc ttttttgttc cataccttat
    11941 tttgcatcag cctttactgg tttaacagag ccgccccatt tgtctgtgat ccattcttgg
    12001 acttctttgc ggtgaagcac ttcaatcagt tttttgattt tgtcactgtc ttcatcacct
    12061 ttacgagtcg caataatatt cacgtaagga gagttgtcct tttcaagcaa tacggcatct
    12121 tctaatggat tcaaccctgc gccgtaagcg aagtttgcgt tgattgcaac taggtcacct
    12181 tcatcatttt ggtacgtaga agctaaaagt gatgggtcga tcgtatggtt gaacttcaag
    12241 ttcttagggt tttcttcaat atcatcgaat gttgctgttt ccagatctac tccatctttg
    12301 actttgacta agccagcatc ttgtagaatt gtgatgatgc gtccccagtc tgattcagaa
    12361 tttgaagtaa tgattgttgc accatctttt aattcggaaa catctttgac ttttttagag
    12421 tacaagccca ttggttcgat gtggacagca ccggcattga caaagtcata tcctttttct
    12481 ttgatttctt tgttcaaata aggaatatgc tggaagtaat tggcatcaat gtcgccttct
    12541 tccaaagatt ggttcggtaa tacatagtca tcgaatttga cgatttcaag atcgattcct
    12601 tcatctttca ataatggttt cacatgttcc aaaatctctg cgtgcggtgt cggtgaagcg
    12661 ccgactttca atgtcgtgtc gtctgcttta tttgctgaat ctttgctgtc gctgttgtta
    12721 ccgcatgctg ctaatcctac agtcagcaaa agcgctgctg caaatccaaa aattttcttt
    12781 ttcatatcct acatccccct atatcctatt atcttttgtc tgttttctta gttaagaaat
    12841 caccgattcc ttgaacgata aatacgatta gtaaaataat taaagtcgca acgaatgtca
    12901 ctgtcagatt accacgctgg tagccttcct gccaagcaag acctccaagt ccacctgcgc
    12961 cgatcgcgcc ggccatcgca gtaaatccga tcatcgagat cgttgtaact gttaatccag
    13021 aaatcaatgc gggaatactt tcaggtatca agaccttcca gatgatttcc cagtaacttg
    13081 ctcccattgc atctgctgct tccagtacac cagggctgac ttcacgaaaa gcaatctcta
    13141 ctagacgggc ataaaatggt gctgcggata aaacaagagc tggaatggct gcttgcgcac
    13201 ctagcatagt gcctacgatt gctttagtaa atggaatgat caaaaccatc aggatcataa
    13261 atggcgttga ccggaaaata ttactgatga tcgatacgat tccataaatg attcgagcat
    13321 acggtttgtt tttgcgtcca atagagtaaa ggatcaatcc caaaactaat ccgatgatcg
    13381 tcactaagac cattgagatt aatgtcatat atagtgtatc aaggatagcc tgattcatcg
    13441 tttccgggtt gatttgttta aaatctagat atgtttctga aaagctttta tccacgatgg
    13501 atcacctcaa ttcctactcc ttgttctttg atgaacactt ctgcttgatc taattctttg
    13561 gtttcaccag tcatcaagac agtcaatgaa ccaaaggaac cttctttcac ttgtttgatc
    13621 tttccataca caacgttgac atcgatgtta tagcgtcgaa tcgcttggga aatgactggc
    13681 tcgttggcat ttctttcgct aaaggtcaaa gttgccaaac ggccgcttgg attttctttg
    13741 atgagttcag ctaatagttc tgtcgaatca gcttctggtt cgatgtcttg ttgtacaaag
    13801 cgtttggtta cttctttttt cggttgtctg aagacagtca atacgtctcc ttcttctacg
    13861 actttcccca actccatcac agcgactttg ttgcagattt tgcggatcac attcatttca
    13921 tgtgtgatca agacgatggt caaattcaat ttcttattga tcgctaacaa taagtctaat
    13981 acttcttctg ttgtttgcgg gtccaatgcg ctagtagcct catcacaaag caggatctca
    14041 ggatcatttg ccaatgctcg tgcgataccg acgcgttgtt tctgcccgcc ggaaagttgt
    14101 gaaggatatg cttgtccgcg tccttctaac ccgactaaac gcaacagttc ttctgcccgt
    14161 tctttacgtt tgctctttgg tacgccagct aattccagcg gcagctggat attttcaagt
    14221 acagttcttg accataacag gttgaaatgc tggaagatca taccgatttt ctttcggaat
    14281 gtacgtaatt ctttgttttt taattttgta atatcttgat cgtttacgat cacttcgccg
    14341 ctcgtgggaa gctcaagtcc attcaggaga cggactaatg tactttttcc tgcgccagaa
    14401 tagccgacga ttccataaat atcccctttt tcaacgttta gtgaaacgtc attgacggct
    14461 tggatgtttc cgtttttccc attgaatgtc tttttgacat tcgttagttg gattaatgac
    14521 atgccattca catctttctc ttatgatttt tcaaacaaaa aagccttcgc cctcttgcct
    14581 taagcaaaag gacgaaagct tgaacttccg tgttaccacc ttgatttacc gttacttcgc
    14641 aataacagcc tcttccagta cttggactta gcttctttcc gtatactgtc gcgctgtaac
    14701 gggcactccc gtagtaagtt tgcttacgct cgcttactct gctctaagac catcttccat
    14761 ctcattttcg ttacccattt tcagcaaaca gggctctctt caaacgtccg tgacatgtac
    14821 tcttcttttc aaagctttct cgtttcatat aggattgctt atgattatag caactggaag
    14881 aatcagtgtc aacttttttc aagtgatttt ctgattttat aattcgtcaa attcttttgg
    14941 atcaacatct ttgaaactta agatccacgc attcatttca tctgaatcat tcaagatatt
    15001 gatatcttca ccgattttat cattaacaga agaaactgta cccgttagcg ggctggcaaa
    15061 ttcattgacc gctttttccg cttcaacttc gatcagaggt tcatcttttt ttagcgattg
    15121 tcctgctttt ggcagattgg caaacgtgat gtctccaagt tcttcttgcg cttcatttgt
    15181 caaaccaaca cagtattctt ttccattaaa taagatccaa aggttgtcct ttttcttcaa
    15241 atgcttgcct gccattttat atcagttctc cttcataaat tttttcattg aatccaattg
    15301 caacaagctg gtcctctcgt aagatcagtg gccgcttgat cagcatacca tctgtcgata
    15361 agacttggct cgcttttgaa gccgtaaact cgcctacttg atctttcagt ccaagttccc
    15421 gatatttcat cccgcttgtg ttgaacaggc gacggattgg tacttgactc gtttctagcc
    15481 aagtctcaat cgttgaggcc ttcggtactt ctttgatcat atcaacagtt tgataactaa
    15541 cgccatgctg gtctaaccac gctttggctt ttttgcatgt tgaacatttc gggtaccagt
    15601 aaaaggtgta caattttctt acctccttga agatcttgca ggtgcttcat tttggtatct
    15661 catcgataat atcaacccga taccgatcat gttccctaaa atcgaagatc ctccctgact
    15721 aatgaaaggc aatgggatcc ctgttaaagg aagtaatccg atatttgctc caatattctc
    15781 aaagacatgg aacagcagca tcatgatcaa accgcttgcg atataggcgt aaaattcatt
    15841 attcgtatcg aaacacaccc gaatcatacg atagattaaa ataaaataca agaaaatgac
    15901 aaatgcacca ccgataaagc cgaagttttc accgacaacg gtaaaaatca tatcggactc
    15961 acgaacaggc acataaatat tgcttacgtt gaatccttta ccaaacaagc caccggtacc
    16021 aattgccaaa agtccttggg ccggctgata actcattcct gacgtatcat ggaaagggtc
    16081 cagccatgaa tcaatacgtg caaactgata agatttgaag ccaactttgt aaagcaagtc
    16141 tcttccgcct tcagtcgtaa ccaagaagat cgttcctgct ccaacgacta ctgccaagat
    16201 tactacaggt gcgatgatgc gccaagagat tccagacatc aagaaaatcc cgccaaagat
    16261 cgcaagaaag acaagcatcg ttccaaagtc atcttgggcc atgatcaatg ctaaaaccgg
    16321 tattgtcacg atcaagagct tcccaatcag ccaaaagtcc gatttcatcg tccgttcttt
    16381 tacttgcgta ttatgtttag ttacgatcag tgccatcatc aagatgtacg caatcttcat
    16441 caactctgct ggctggaaag taaacgcacc gaatcgaaac cagtttttag accctgttga
    16501 agcagctaaa ttctgatcat agaatttgag caagagtccc attaccacta accccaaacc
    16561 ataaaagtaa ggagtcagtt tccagatcca tttggaattc aaatgcatga tgacaacgat
    16621 tgctgccgct ccaactaaat accagacaac ttgcatccct acgcctttca agactgctgg
    16681 attgttcgga tcatggctaa gtgctacata taatgagagc agaccaatca aacataacag
    16741 aaagactggt aaaatcacgc cataatcaat acgattatcg ttcgaagctt ttaatttttt
    16801 ttccatgttt catccctttt cttaaaaacc atactgctga ttattatagc ggtccagtta
    16861 gataatggaa agtctttcca tagacatttg ggaaaaatta actttccgtt tgtgcagttg
    16921 cttgagcaaa tgcagtacaa aagcctactg cttatcttta gtgtatcata gaactttttt
    16981 tgatacatgc aattatctac caactgagat tctcctcgct gtgagaagtt tctgtgcttt
    17041 cattgtatcg aaggatctct tcggcaatct ctagcagctg atcagaaacc agattggttc
    17101 gttggatgaa ataaaaataa cgatggaaat tcaacggagt cattggcaca ttttctggaa
    17161 cagagcgctt agacaaaatc gagcgaccga ttccttcctt cagcaacgca acaattacat
    17221 cgttactttt cacgatcatc ttcttatctt ggatattcat ctcttcaaaa taacgctgcg
    17281 tgtaatgaaa aacaccagag tttgcttctc ttacaagcca caatttgctt tttgggtccc
    17341 cagcgatgac tagctggtct tccatcaaag agtatctttg tacgccggaa gtctctaatg
    17401 gcttttcgat aaaaccaaaa tcggattcat gtttcgctac ttgttctaat gattcatgag
    17461 aattcgcgat ttcgatctca aaatgcactt taggaaacct tttgatcaga tgtttcatca
    17521 actgaggcaa aggatagaca gcaaaggtgt gggaagcaac gattcgacat acttcttctt
    17581 cattttgttc attgagcaat ttttgctgag tttcttccca ttcttctaac aaaaccagtg
    17641 tccgttcata taataagtcg gcttgcttag ttggaagcat ctcttttctt ccgttacgtt
    17701 gaaaaagatc gatattcaat tcccgctcca actgtttgat ttgattcgag actgctggtt
    17761 gagataaaaa cagtacttct gctgctctgg aaaaatttct tgtttcatat actgctcgaa
    17821 aagtcgttaa taacttaaac atggcaccac ttccataact ttttatgata attactataa
    17881 aaactatttg atttaaatat acaaaaaacc atctataata gaaatgtaca acaaaacagt
    17941 tgctgatcca actagtaact tagatgaaga caatcagctg tctgcttgct cgtaatcata
    18001 cattcctatt cattttacaa tgtattcgtt catcttggta ctaaaatcta ttaaataaaa
    18061 aggagaatat tatatatgaa gaactttttg aaaattattc cgatcccgat ctgcgggttg
    18121 atccttgggt tagcttccct tggaaatctg tttaaagact atcaccatgt tgctttaggc
    18181 aatctaactg gcggtatctc tatgattctt atgatattca tttttgttaa attgtttatc
    18241 ttattcgaac acacgaaaca aacgttgcat gatccaatca tcgcctccgt ttcaccaact
    18301 tttacgatgt cactcatggt tatttgtact tattttgttt catatgaaag tattgcacca
    18361 attgtcaaat tcatttggct gtgcgctgtt atcttccaag tgatccttgt cttttatttc
    18421 aattatcacc atgttatcaa agctgatctt tcaattgaag caatttatcc aagctggttt
    18481 atcatttatg ttggatttgg ggtcatcact gtaactgccg ggaacttctt cccgcttatt
    18541 ggaaaaattt tcttttggat ttcattgatc tgctatgcgt tcttattgcc gatcatcatc
    18601 aaacgtgtct ttttcgtaag aaatatggct catccgacac gtccgctgat tacgatcgtt
    18661 gcagcacctg gttctctttg cctaacaggt tatcttaaaa actttgctga acctaatttt
    18721 tatcttgtcg tagcattgtt cgctttatct caagtattgt atttcgttgt gctgtctatg
    18781 cttccacgtt tgttcaagct tcgtttttat ccaagctatg cagcctttac attcccgctt
    18841 gttatctctg caacagctat atttactact gttcattact tcaacagttt aggtatctat
    18901 agtagcgtat tggatttctt aaaagttgcg gaatgtctgt ttgcaagtgt agttgtcttc
    18961 tacgttcttg ctcattacgt acgtttctta attaaagaac atagcaaaca tggaaaattt
    19021 gtttctaaaa aaatcgctta ataatcgatt caataggttg tgaaaaagct gttctgcatc
    19081 aagtaataag aacggccaaa caaaaacagc tgttcatgtt tttgaattgg ttgggcttat
    19141 taccgcagat gcaagctttt gaacaccgtt taccaagagg tcgtaaaaga agtggccagc
    19201 ttcaagaaat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga aatttcggct
    19261 tatttccgaa aaagctactt ctggaacacc gtttaccaag aggtcgtgaa aaag
//
LOCUS       DACCCR010000051        19308 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCCR010000051 DACCCR010000000
VERSION     DACCCR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.058"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            968..2218
                     /locus_tag="IVK93_001805"
     CDS             968..2218
                     /locus_tag="IVK93_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP6387093.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2237..3247
                     /locus_tag="IVK93_001806"
     CDS             2237..3247
                     /locus_tag="IVK93_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP6387094.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            3257..3910
                     /locus_tag="IVK93_001807"
     CDS             3257..3910
                     /locus_tag="IVK93_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6387095.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3929..4927
                     /locus_tag="IVK93_001808"
     CDS             3929..4927
                     /locus_tag="IVK93_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP6387096.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            5003..5764
                     /locus_tag="IVK93_001809"
     CDS             5003..5764
                     /locus_tag="IVK93_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP6387097.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5782..6888
                     /locus_tag="IVK93_001810"
     CDS             5782..6888
                     /locus_tag="IVK93_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6387098.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6878..7111
                     /locus_tag="IVK93_001811"
     CDS             6878..7111
                     /locus_tag="IVK93_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387099.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7310..7765
                     /locus_tag="IVK93_001812"
     CDS             7310..7765
                     /locus_tag="IVK93_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP6387100.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7779..8552
                     /locus_tag="IVK93_001813"
     CDS             7779..8552
                     /locus_tag="IVK93_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6387101.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8539..9669
                     /locus_tag="IVK93_001814"
     CDS             8539..9669
                     /locus_tag="IVK93_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6387102.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9700..10935
                     /locus_tag="IVK93_001815"
     CDS             9700..10935
                     /locus_tag="IVK93_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387103.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10960..12069
                     /locus_tag="IVK93_001816"
     CDS             10960..12069
                     /locus_tag="IVK93_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387104.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
     gene            12111..13445
                     /gene="gmhB"
                     /locus_tag="IVK93_001817"
     CDS             12111..13445
                     /gene="gmhB"
                     /locus_tag="IVK93_001817"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAP6387105.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13480..15117
                     /locus_tag="IVK93_001818"
     CDS             13480..15117
                     /locus_tag="IVK93_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP6387106.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            15219..15614
                     /locus_tag="IVK93_001819"
     CDS             15219..15614
                     /locus_tag="IVK93_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP6387107.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15632..16363
                     /locus_tag="IVK93_001820"
     CDS             15632..16363
                     /locus_tag="IVK93_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387108.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16387..17838
                     /locus_tag="IVK93_001821"
     CDS             16387..17838
                     /locus_tag="IVK93_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP6387109.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            17864..18475
                     /locus_tag="IVK93_001822"
     CDS             17864..18475
                     /locus_tag="IVK93_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6387110.1"
                     /translation="MIDIISAIMVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            18657..19172
                     /locus_tag="IVK93_001823"
     CDS             18657..19172
                     /locus_tag="IVK93_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAP6387111.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNGERLSF"
BASE COUNT         6223 a         2699 c         4217 g         6169 t
ORIGIN      
        1 ttaccaacat gtttagacat tttcctctag gaaaaaatta actagcacca cgggtgatat
       61 atatgtatat ggtgactgaa gacaaaaaaa cagaactaat gaaactgaat aaaaatatag
      121 agtgaactag tttctaagag ggattttaaa ctagatttca ttggtaataa aatttggaga
      181 gatggaaaaa cagcacttaa ggaagcagat tttgttgtca tttttgttcc tacaaactat
      241 ggtagtgaat gtgttattga attagtgatg tctgttattt cagaggcatt ggggataatt
      301 aaatccacta tacctgtagg atatacaaaa agtggaagaa aaaaatatta tcaatagatt
      361 ttatttagtt cagaattttt acgtgaaagt agattattaa tgataatctt tatccaatta
      421 gaattattat cgtaggatgt gatgatgaaa gccgtgaaat tgttcaagta tttaccaatg
      481 tctccctgcc agtaaatccg cttagaattt gagccttgaa aatgtgcaat gataaaaaac
      541 aaaaactcgc tcaaaataga cagtttgggg tgggaataga ccaataatgt accagacaaa
      601 cttccatttg gggtgaaaat aggtcaataa agcgatgtta aacatacctt tggagtaaga
      661 atagagggta ataagtgaat ttataaactt tttggtgact ggttgagtgg aaaatgtagg
      721 tacatttcaa tgcgaaaatg tattataacc ccaaacgagg tcaataaaaa tgactcaaac
      781 ttgcaatata accctaaacg ggtaggggat ttatgaaaaa ttgaatttgc cggcgtagga
      841 gctggctgtt ttaagtatga atgaacacag ctttcgggct gttttatata aatagcaggt
      901 tactgttcac aaacagcatt ttgttaagaa agtagtgacc acagcagtgg tggaaagagg
      961 aaatattatg aagaaacagt ataacattgc agtcgccggc accggttatg ttggtttgag
     1021 tattgccact ctgttggcac agcaccataa ggttacagcg gtggatatca ttccagagaa
     1081 ggttgaactg attaataacc gtaaatctcc aatccaggat gattatattg aaaagtacct
     1141 ggctgagaaa gaactgaatc tgaccgcaac attggatgca aaggcagcat accagtctgc
     1201 ggattttgtt gttattgcgg ctcctactaa ctacaatagc cgcactcaac attttgacac
     1261 atccgctgtt gaggctgtta ttaggctggt catggaagta aatcctgatg ccattatggt
     1321 tattaagtcc accattcctg ttggatacac cgcttctgtt cgtgtaaaga ctggtagcaa
     1381 gaatattatt ttcagccctg agttcctgcg tgaaagtaag gcactgtatg acaatctgta
     1441 tccttcgcgt attattgtgg gtaccgatat ggatgatgag cgtttggttg aggatgcaca
     1501 cacttttgct acactgctac aggaaggcgc aatcaaagag aatgtggaca cattgttcat
     1561 gggctttact gaagcagaag ctgttaagct gtttgccaat acatatctgg ctcttcgtgt
     1621 cagctacttc aatgagctgg acacttatgc agagatgaaa ggcttgaaca ctcagcagat
     1681 cattaatgga gtttgccttg atcctcgtat tggtactcat tacaacaacc cttcctttgg
     1741 ttacggtggc tattgcctgc cgaaggatac caagcagctt ctggccaact acgcagatgt
     1801 tcctgaaaat ctgatcgagg caatcgttga atctaaccgt actcgtaagg actttattgc
     1861 ggatcgtgta cttcagatgg ctggttatta caattacgca gaagcaaatc agtatgatgc
     1921 tggcaaagaa aagcctgttg tgattggtgt ttaccgtttg accatgaaat ccaattctga
     1981 taacttccgc cagagttcta tccagggcgt tatgaagcgt gtaaaagcta aaggtgcaac
     2041 tgttgtgatt tatgagccga ccttgaaaga tggagagact ttctttggca gtaaggttgt
     2101 gaatgatttg gaacagttta aggcaatgtg tgattctgtt atagcaaatc gctacgatgc
     2161 ttgccttgat gacattaagg aaaaagtata tacccgtgat atcttccaga gagactaaga
     2221 ttaggagaga gtatttatga ttattacaaa aacaccgttt cgtatgtctt tttttggcgg
     2281 tggaacggat atggaggatt atttcaaaga gaatggcggt gcagttcttt ctactacttt
     2341 tgataagtac tgttatgtga atgtgagaca tctgcctaga ttttttgatt acagcgctga
     2401 gttatcttat tctcgtactg agagagtaac tagcattgaa gatattgagc atcctgcgat
     2461 tcgtaatgcg atgaaaatgc tcgatgtgca tgaaattcgg cttacatacg aagctgattt
     2521 acctgctcgc tctggtctgg gtacatccag ttcttttgca gttggtatgt tgaatgcttt
     2581 ttatgcactg gagggtaagt atgctgacaa gaagaaactt gctgatgaag ccatttatct
     2641 tgaaagagtt ctgtgtaatg aagccggtgg atggcaggat cagattgccg catcttatgg
     2701 tgggttcaat cgaattaatt tcaatgtgga tggttatgag gtgctgcctg tcattatttc
     2761 gcctgaacgt aaaaagcagc ttaacaacaa tctgatgatg ttctttaccg gattcactag
     2821 attttcttct gatgttcaaa aggctaacaa cgtcagtggc acagaggata agagagtaag
     2881 actgaaaaag atgtatgagc ttgtagatga agctgaggca attcttactg ataagaacag
     2941 aaatttagat gatttcggta gacagcttga tgttacttgg agattgaaaa aaggtactgg
     3001 tggagcaatt agtacaggaa gcattgatga actatatgaa aagggtatgg ctgcaggtgc
     3061 tttaggcgga aagttgctcg gtgcaggtgg tggaggtttc ttggttttct atgtacaacc
     3121 tgagaaacag gatgcagttc gttgggctat gcgtgatttg atgtatattc cgtttgaatt
     3181 tgaagatggt ggaactagag ttattcacta cacgccggaa acttatattg aaaaacagaa
     3241 tggataagga gtaacaatgt tagcggaaaa acttaataaa catattgact tattgattaa
     3301 acgatatcct acacttgaga cgattaaaca ggacattatc gatggatacc taattatgga
     3361 ggaatgctat ttgaatggtg gtaaacttct tgtggcagga aacggaggat cagccgctga
     3421 ctcggaacat attgctggcg agttgatgaa gcggtttaag acgccacgtc ctgtaagtaa
     3481 ggaatttgcc gataagttag ttgcaattga ccctgtaaga ggtccggaat tagccaaaaa
     3541 tctcgagtgt ggtttaacgg caattccttt ggtcgcacac gaagcattgt ctactgctta
     3601 tattaatgat gtcgatggtt tgggtgtctt cgcacagcag ctgtttggct ttggtagatc
     3661 tggtgacgta ttccttggta tttcgacgtc cggaaatagc aagaatatta tgagcgcaac
     3721 tgttgttgct agggctctcg gtataaaagt tattggcttg acgggcgcaa agggtgggga
     3781 acttgccacg gtagcagatg tttgtgttaa ggctcctgaa gttgaaacat acatgattca
     3841 agagtatcac ttgccgatat atcattgttg gtgtttgatg ctggaagata aattctttgg
     3901 aaacgaataa aatttggagg aatgttaaat gaaaaagaca attatggtta ccggtggttg
     3961 cggtatgatt ggctccaatc ttgttaagag actggtgaag gaaggctgct gggatgttta
     4021 tgtagctgat aatctgtgga gaggaaagtt ggagtacctg aacgacgaag atgggcatcc
     4081 tgtcatcgac ctggatactc atttcttcaa tgctgatctg actgattatg agcaggctaa
     4141 aaaggttatt ggtacgactg agtatgtagt tcatctggct gatgttgtgg ctggcattga
     4201 ctatgtgttt aagaaccagg gcgaattgtt ccgaatcaat aatctgatca attctaatgt
     4261 ttttgattgt tgccgtaagg ctggcaagga gaaaatcaag ggtgttatct atgttggtac
     4321 cgtatgctcc ttccctctga ccagacagaa tactttgaat cctgagcctc tccgggaaga
     4381 ggagttgttc cctgctcttc ctgagtctgc ttatggctgg agcaaactga tgggccagtt
     4441 ggagatgggg tatttggaaa aagaaaccgg aattccgtgc tgtatcctga tgcttcataa
     4501 tgtttatggc acacctactg attttggcga acgtagtcag gttattcctg ctctgattcg
     4561 taaagcaatc aataatcctg aagagccttt taatgtgtgg ggtagcggag agcagggacg
     4621 tgctttcatt catgtcaatg atatcgtaaa tgcattggtg ctcgccctgg ataagggttg
     4681 gggtcatggt catattcaga ttggcccttc tcactgtact tccatcaagg aaattgcata
     4741 taagatcatt gaaatttctg gcaaagacat caagcctttc tttgatactt ctaagcctga
     4801 aggtgataag gctcgttgcg cagattattc taaggcgaaa gagattttgg attggaagcc
     4861 tactgtttct ttggaagagg gtcttcgtga atcctacaat tggattgaaa agcagattaa
     4921 gggttaataa gtatataagc tgttcttcct ccgtgtaatt agaggatgcg gaggaagaac
     4981 atatgagtaa ggatgtactg agatggaaaa gaatatgtat aaaagaaaac gtgatttcac
     5041 tgtgtgtgga atagtaggtg tttgtgctac tacaaagaac gagtttgtaa gggaagtttt
     5101 ttccatgcca acatctccgg taagtgcgac tgttgatctc gtgggagtgc cggccattat
     5161 ttcggcaaag gaaaatccca aaattgctga gatgtatagc caggctacaa tggctgcaat
     5221 agatggtatg cctattgtta aaattggacg gcgcaaagga tttacctgtg aacgatgtgc
     5281 tgcaccagat attatgggaa tggtttttga agaaggcgtt agacagggaa aaacacatta
     5341 tttctatggt ggaaaagata acgaagtgtt aaataagctt cgtgcaaacc tagaaagaga
     5401 ttacccggga atcaaaattg ttggaatgta ttccccacca tttcgtccgt taaatgaaga
     5461 agaggatagc aagatttgcg aggaaatcaa caacttaaat ccagattttc tgtgggttgg
     5521 tattggtgcg cctaagcaag aaatgtggat gtgggaacac cagaagaaga tccataacac
     5581 agtaatgttc ggtgtaggcg ccggctttaa cttttttgcg ggcactttgg aaaaagctcc
     5641 tgaatggatg gaaaaagcta gtctagagtg gttatttaga ctggtaaagg aacctcgtag
     5701 actatggagg cggtatatct tgggaggtat aaagtatatc tactatacta ttgaatctaa
     5761 gtaggggggc aataaaaata gatgaaaata cttatagtag ttacaatgac gaataacttt
     5821 ggtaagaagg ggtcctataa ttctcaggaa ataggattag ctcgagggtt agctgaatta
     5881 ggccacgaag ttattgtata taaaagtgta aaaagtgatg aggaagttgg aacagagcaa
     5941 ttaagcaata cggcgatgat tcactatatc aagtgtcgtt atgtaggatc aaatggattc
     6001 ttatcaacaa agaattttga taagaatgcg gatgttctaa tacaatttgc ggaccttcaa
     6061 ctatcagtac ctcatgtata ctcatggact gtaaaaaatg gaataaaata tataccttat
     6121 aatgggataa cgcgaagtca aagtggcaat aaatatataa agacaattat tgaaatattg
     6181 gcgcatcgaa attataaaat ttataagaaa aatgggtgtg ttgccaaaaa tgatcgagtt
     6241 tctgaaaaat tagtacaatt aggagtaaat aaagtgaaac ttgctcctgt ttgtgtggat
     6301 tttgaacagt tacataagaa ttacgaagca atagatttta ctactctcag agaaaaatgg
     6361 aatattcctc gcgatgttcg tgttatttta tatgtaggac ggtttgaaaa ggataaaaga
     6421 ccgttagata tgattagttt atttaataaa cttgatgagc ataattattt gctgatggta
     6481 ggaactggtg ctttactcga gcaaacgaag cagcagataa atttacttgg actgcagaat
     6541 agagtaaaaa taattgagcg tattcctaac aaagatattt gggagttata ttgcttatcg
     6601 gatgtatttg taaatctaaa ctatactgaa atttggggta tggttttgct agaggcaatg
     6661 tactatcgcc tcggaattat tgctgttaat gcacctggcc cacagaatat tattttggat
     6721 aatagatatg gaatgttggc taaatctgac gatgaaattt tgacattctt aaaagacgga
     6781 cgatttacca acgatatcat agatgcagca tatcagcgtg taactacaga atttagatgg
     6841 tcaaagtcgg gagaaattat gcttagcatg ttggaaggtg ataaatgata tggaagtaac
     6901 gaaattaatg gcattgagga atcgatatgc tttaaacata gttgataatt gcacgagaaa
     6961 aatagccaaa attcttggtt gttgtattgg taaaggtgca cagataggga actctgttga
     7021 atttgttcat aattctgtag gcactgtgat tcattctgac acaatccttg aagatggtgt
     7081 taaagtttat cagaatgtga cgtgtggttg agcagatagt aatgaacgta tcatcattcg
     7141 agtaaaaaag aacgctgttt tatgtgtggg tgctaaaatt ctttgtaaat ctaacacaga
     7201 attatgcgtt ggggaaggtg ctgtagttgc tgcaaataca gtggtattga ggtctattcc
     7261 accctacgaa gtgtgggggg tacctgctaa aaagatgggg agataaaaca tgaatgcata
     7321 taaattatac aaagtagaac gatggtttta cttacacaaa atggagtttt ttgccaagat
     7381 tgtaaaggca ctaatttatg tacttcataa tagctcaatt tcatatcaaa cagaaattgg
     7441 agataattgt aagttcttat acggtggcat aggttgtgta attggaaaag atacaaaaat
     7501 aggtaatcac gttataatcg gaacgaatgt actgactggt ggtagatcca ataagcctgg
     7561 tatgcctaca attggaaaca atgtatatat cgctactggc gcaaaaattt taggtaatat
     7621 cacaataggg gacaatgtaa tagtaggggc taatgctgtg gtcattaatg atgttgaacc
     7681 taattgttcg gtaggaggcg ttccggctag agtattgaaa aaagatattg atgtaaatga
     7741 ttattgtagt ttgaatcgtt actaaaagga gagattgtat gttgtatttt gttattcctt
     7801 taatgagtgc gaaggcagca aacaattggg agatggttag taccttattt aaccgaacat
     7861 tatggtcgtg ttataatcag actgatcctg atttcaaaat cattgttgct tgccatgaaa
     7921 ttcctaaact cacgcatgaa tatgatgaca gagttgaatt tattcaagtt agcgaaaagg
     7981 atgcccctat tcccacaaat caagacgaaa aaatgattga taagggttat aaaacacatg
     8041 ctctcgcaat gcgtttgcga gagttgggcg gcggtttcgc tatgatggtt gacgcagatg
     8101 atcttgtaag ttctcatttg gcggaatatg tgaattccca tccccaggaa aatggatttt
     8161 atgtcaaaac gggatacgtg tactttgttg gagacgatta tatgaaggtt ctaccaaagt
     8221 tctcatcagg aagtgcgtgt atagtaaaat atgcagtgga agacctgcct gaggcatatc
     8281 cggaagttat gacagcaaat tgtgatgata atccatggat cattagaaaa cgacatgggg
     8341 gcgttgttcc agcatgtgaa gagcaagggc gtccattaaa acctcttcca ttcaaagcag
     8401 cagtatatgt attaggaaca ggggacaatc actcgcttta tggtaaaacg acaaaatatc
     8461 agacgaagat gcgagagata ctggaaatgt ttatagcaaa acgtaaaatt aaaggaaaac
     8521 tgaaagcaga attttctgtt gattggttat aaggtgagta ttatgaaggt attgatgtgt
     8581 gaaagccaaa tgccagagaa ttggtattcg tttgatcttt ctaaagaact taatcaatat
     8641 gttgatttaa cattgctgtg taagcgtgat gttcctcctt tagatcctaa aattaagagc
     8701 aaacatattt tgtatcataa aagtacaaat aaagtgtttt cggcacttgt ttttctgaaa
     8761 agcttgttct taatttggaa ggaaataaga tttggtgatt atgatattgt tcatttgcaa
     8821 ttgatgaaaa gtctcaaata tgaaggcgga ttgtacgttt tatgtaagag acactataaa
     8881 aaattaatta tgacagttca taatgtgtta ccacatgagg ttcgtgaaaa ggatcgccaa
     8941 gagcacttta atatatattc aaattgtgat ggtttcattg ttcacaatcg agtaacagaa
     9001 cagcatttat atgctgaatt cccagaatta aatgcaaagt catatgtttg tccacatgct
     9061 atctatggag atgttaaaca tatatctaga gcgtatgata aagataagtt tgaatttatc
     9121 ttgtttggac aaattcgtcc atataaggga attgatgtac tgcttaatgc aatcacagaa
     9181 cttccaatgg atgtaagaaa gaaagttcat tttaccatag caggtcaaca acatcctaaa
     9241 caggatagta ctgactataa aaaaatgatt gaagatttaa aggtttcaga ttgtgttcta
     9301 tataagccag agagaatttc tgatgaagaa atggacagta tgttcagaaa tgcagatgcc
     9361 gcaatatttc catataaaga aatctatgga agtggagcgc ttcttatggc atacacttat
     9421 tgcttaccaa ctattgttag taatgtacct gcttttatgg aagaaaccga ggaaggaaag
     9481 atagggttac ttttcgaaaa tttgaattct aagagcctag cagacacaat tgtaaagtat
     9541 gtgaacctta gcccagctga gaaaagcaaa atagaggatg atatatatga gcttgtaaac
     9601 cgcaagtata cctgggcggc atctgcaaaa ctcacatacg aggcatacaa acaaattttg
     9661 aatgagtagt aaaacaatta gaaaggaata ataattaaca tgcagattag taaatggaaa
     9721 atcatacagt ttctaatggt ctttctaatg attatctcat caagcatatt gaagcctttg
     9781 ggcggctggt tgggagaaac gatgaacgtt agacttgtga cagtcattct agcaatagta
     9841 tgtttgttaa tagcctcatt accagcattt agaaaaaata gatatttaaa tttctgcact
     9901 ttactgattt gtgttgttat aatagcagct tacatctact cagataagac aatatgggtt
     9961 aaatctaggg aaagtatgag tttatactat gtattattgg cgtatccatt gttctgtact
    10021 ttaattaacc aagcgtggaa tttggattca atgctaaata cgatatgtgg tgtaacattt
    10081 ctttcataca ttcttagaac atctatttca cttattcaga aattttcagg agtacttctc
    10141 tatgaaaaca tatatcgtga gtgtgccccg gaaaattggt atcgtggagg ctttttaaga
    10201 attaatccac catgttttac aattataata ataccgattg ctttgtatat gattgagcga
    10261 caagttgtag ctagaagaaa agtaaaatat tatttactga ttgctagcgc actggcatat
    10321 tctgctgtta ttcatggttc tagatctgtc cttgtctatc aaattattgt acttggttgc
    10381 tatatccttt ttaagaaagg ttcttcgaaa agaaaaattg caatgtgggt tttagctggg
    10441 ttgcttgtag tcatatttat taattcgacc atgttctcaa catttgttga aactttcaca
    10501 aacagtacag gtgaatatgc aggatctgct gaatctcgat ttgcttcagt ttggtttttt
    10561 gttcctaaat ttttgcaatc gcctttgttc ggtactggta ttcttactgg tgaggaattt
    10621 acgtttatat taccaggagg agttagagga cacctatctg atattggatt tttctataca
    10681 attactcaat atggtgtagg tatacttata tttgatattc tgtttattat aaaagggttc
    10741 aaaacagcat tacttgcatc aaaagtgggt atgactggct atcaatattt agcatttgga
    10801 attacattaa gtgttttgct aacagggatt aatatagact ggttttatcc gatatttact
    10861 ccagctattc ctgtatgctt agcggttata gaatacatct atcaagaatg tagaagtgtg
    10921 gctaacatag aataggaaat attaatagga gtgcatctca tgaataagaa agagatttta
    10981 tataacatat tgaagaagta cgaaaattta ccatatgtgt ttaggaacat gtttggtcat
    11041 aatataagta aactcgtatc ttggaaattg actgctgatt gtgcaacagt agaagaaaat
    11101 tctgaagttt atgcagcgtt tcaattgcgc tcaaaggaca tgtctgatgt tccagtgatg
    11161 ggtttagtta attgttgcaa agatgtagca attgtaatgc aggggccact atgtttaaag
    11221 gatgatttct cttataatac tctggcctac tataagaaat gttatccaaa tgctttaatt
    11281 attctttcga catggaagga cgaagatatt aaggcattga aaaggataga ggatcttgga
    11341 gttatagtta tttgtagtga gaaacctgaa aagcctggac acttaaatat taattatcaa
    11401 gttggaaaca ctttgcctgg aatagtcaga gcaaagcagc ttggtgcaaa gtatgtttgt
    11461 aaaactagaa cagatcaaag aatataccat ccaaatgcaa tggcgttttt ctgcagttta
    11521 cttgaacagt atcctgttaa taacgaagat gattggaaat taatacaaaa tcagcgatta
    11581 ctgttactat caatgccgta tggagacatg tttttcccgt attgtttgtc agacttcctg
    11641 tattttggtg atgttgatga tatgataaac ctcttctcga ttcctagtga tgtgagagaa
    11701 aagggagctg tgtctagagg agtctcaaga agggagatat ctgaaaataa tttagctcca
    11761 gaagttcagc ttcttcgaag ctatatttca cgaatggggg gcaatgagga gtgctctatt
    11821 agggcatatt gggagtttgt aaaaaaccat gttattacta ttaacaaaaa tcaaattgat
    11881 ttgtactggc ataaatatgt aggtcgttat tcaaataata ctatttatgg tacgtattat
    11941 attgatgata gccaagatgc acttcactgc tataattttg attttataaa ttggttgaat
    12001 ttgtacactg gaaaatacga atacaaagca aaatatgaaa aatatatgga ctttgtttgg
    12061 gaagagtaat taacttttat agtaaaaagt actttaggga agggcatatt atgaaaacag
    12121 taattatggc gggcggacga ggaagccgaa tttcggaatt gtttccggat attcctaagc
    12181 caatgattcc tatagatggc attccagttt tagaacgaga attgtgttcg cttagagatc
    12241 aaggctttaa tgatattatt ttgactgttt cctatttgca tgaaaaaata gaggagcatt
    12301 tcggagatgg ttcaaaatta ggaattaaaa tcaaatattt tattgaaaaa actccacttg
    12361 gcaatgcagg tgcattgtat tttttgttgg atgaactggg tgatgaaccg tttttacttt
    12421 tgaatgctga tgcagtattt gatgttgatt tcaggcagat gattagattt catgttgaga
    12481 ataacgcaga tgttactttg tttacgcatc ctaactcaca tccgtatgat agtggattaa
    12541 ttatatccga tgacaataag aaagtgcttc aatggttagc gaaggaagat caaagacctg
    12601 aatattataa aaaccgagta aatgcaggtc ttcatgttat aaatccgaag gtgttgagag
    12661 aaaatccatt aatggaaatt gtaaataaat caatagttga aggtgggggg ctaccataca
    12721 aaattgattt agatagaatg attctgaaac cattggctaa cacagggcgt atgtattgct
    12781 acgattctcc agagtatgtt aaagatatgg gaacaccgga ccgatataga gctgtaatta
    12841 aggactttca aaagggaatt attgttggta aaaatctatc caatccacaa aaagcaatct
    12901 ttttagatag agatggaacg attaataaat acgttggttt tttgagaaat gcagaagaat
    12961 ttgagttaat tgatggagta tcggaggcaa ttaagattat taattctcta ggttttctgt
    13021 gtattgttat aacaaatcag cctgtaattg caagaggtga agttactgtt gatgagctta
    13081 atagtattca caataagctt gaaacaatgc tagggcatgc tggtggattt ttaaacggta
    13141 tttattactg tccgcaccat ccccataaag gatatgcagg agaggttata gaattaaaaa
    13201 ttgattgcaa ttgtcgcaag ccaaaacctg gactgttatt agaagcatca aaggatttta
    13261 acataaacct aagtcaatca tttatggttg gagatagtga aagtgatgta attgctggaa
    13321 aaaatgctgg ttgtaagaca gtactgatta cagaagacat caccttgcct gctaattatg
    13381 gccaagacta tagtgtctgt tcattgttag aatttgttaa aaatcattta tgcactatgc
    13441 aataattggt ttagtgaaac ttgtaaaagg agtaattaga tgaaggtatt aattccagca
    13501 gcaaaagttg ttgaaaaaga attgcagagt ataggaatgt taccagcaat tatctatcca
    13561 attaataaaa aaattgtctt tgattatttg tataatcagt ataatgcaat ctgcgattca
    13621 ttggatatta tctgttatga aaaagcggat aaggttcata gaagactgaa aaactatcat
    13681 gattccagaa ttcatatatg tgaccttcct gaattagggg atctagggca aaccgtgtat
    13741 tttgggttat cagatgaaga tgagcctgtt ctaattaact tcgcagatac aattgttatg
    13801 gagagtatgt ctgatataga aatggataca ttctattatt ctgaagattt tgtgtcgagt
    13861 acttggacat actttaaaat taatgaagga ataattactg atatccacga taaagaggaa
    13921 gcttacgtat ccgataaacg tgaaaaatta tttattggcg tatttatgtt ttcttcgtca
    13981 aaacttctga agagttgctt aagagaggca gttgagagtc ggaaacaggg gggaataaac
    14041 tcattttatc gggctttaca gctatacagc gaacgttgtc cgctaaaggc cgtaagaaca
    14101 gaaaagtggt ttgatattgg acacatcgat aaatattaca atacaaagtt ggaagttaaa
    14161 gcacgagagt ttaatcatat ctctatagat aaagatcgag ggatattgaa gaaaactagc
    14221 gatgacaagg ataaatttat tggagaaata aagtggtatt taaaacttcc tgctgatatt
    14281 gaatatgtaa gacctagaat ttttgattat tctacatctt acgtagctcc ctatgttgat
    14341 atggaatatt atgcatatca tacggttcat gagttatact tatatggtga tttaaaatat
    14401 aaccagtggc atgatctgtt tagccgtata aagtttatct gtagcgattt taagcgatac
    14461 accttaaaag atgaaaagat tagagagtca ttggaagata tgtatttgcg aaaaaccatg
    14521 cagagatttc aaaaattgtc tgaagataaa agattttcag ctttttttga gaaacaaatt
    14581 caaattaatg gtcgtaagtt tatttcttta accggtgtaa ttgaacttct aaagaatctt
    14641 atcccaagta tgctctatga tgtaaatgaa ttttgtatta tacatggaga tttgtgtttt
    14701 gctaatatga tggtagattc caattgttcc tttgttaagg ttattgatcc taggggaaaa
    14761 tttggaaaat atgatattta tggggacagc agatatgaat tagcaaaact atttcatagt
    14821 ttagatggta aatatgatta tatcatcaag gatctattta ctgtagaata tgataccgag
    14881 tgtgcagtga tagattatac aattaatacg cgtgtaagag attacgattt atttgaattg
    14941 tttaagtcgg tcttttcgga cgagattaag gacgattttg ataagattgt attgatagaa
    15001 gcgttgctat ttcttagtat gattccatta cacggtgaaa gttttgatca ccaaatagtg
    15061 atgcttgcta caggtatgga acttcttgca agagtaagta ataaagttat actctaatat
    15121 aaattgtgcc ttaatgaata acggttacaa tactgtttta gcaatgtagg cattaaggca
    15181 cgattatatt aattaataca atagaaggga agagaaaaat gtatagtgaa tattcttttg
    15241 tgtttgatat cgatggcact ttgtgtccga taaagaagaa ggatgagcga tatgaggatt
    15301 tggtgccgta tgagaatatg gttgaaaaac tgagattcta taaggagaat ggtgctaaaa
    15361 ttacattgtt cacctcacgc aatgttaatt cctataaagg aaatttggga atgataaatg
    15421 ctaatactgc aaaagtgctc ttggcgtggt tggataaatg ggacattccg tatgatgaaa
    15481 tcctgtatgg taagccttgg ccggggcata aaggcttcta tgtagatgat cgaacggtta
    15541 gacctgatga gtttttgaga tgctctgtag atgaattaaa tgaaatctgc aacagttcaa
    15601 aaaaaagatt ctaaataagg aggttagttc tatggaaaaa aagatgacag ttatcattac
    15661 gatggcaggt ttgggaagtc gttttcgaaa aattggttat gcggttccta aatatatgat
    15721 taatgccaaa ggaagaactt tatttgattg ggccatggat agtttggttg gatacaataa
    15781 aaatgttgat aagtatattt ttgttgttcg tcaggaagac aacgcttcag cttttataaa
    15841 ggagcattgt gcttactttg gtattgaaaa cgtagaagtg attgaaatag actatatgac
    15901 agatggtcaa gcaactacat gtatgttggc tattccttat tgcaatcaag acaatgagat
    15961 tatggtttac aatattgata catacgtgga atcaaatgag atgaaatacg aagatatttg
    16021 tggagatgga catattccat gctttcatgc tgatggcgat cattggagtt ttgcaaagct
    16081 taacgatcga aatgaagtaa tagaagtccg cgaaaaagtt cgcatttctg ataattgtac
    16141 tcttggtgcg tattatttct catcggcaaa gctgtataaa gctttatatg aagagtatta
    16201 tgcagactcc tctaatatgg agaagaatga aaaatatata gcaccattat ataactttat
    16261 gataaaaaaa gggttaaagg tgactattag catagtagat gcgaaaaagg ttcatgtgct
    16321 tggtactcca gaagagctca atgtgttttt gaatcaagaa taaatagtgt ttaggagaaa
    16381 atagcagtga ccagtaataa tctaattgta aaaacattaa gcagatataa gaatactcca
    16441 cttcctgtta aggctgcatt atggtttatg gtatgtggtt ttttacagag aggggtttca
    16501 atgattacaa ccccaatttt tactagagtt ttgtctacag aacaatacgg tttatacagc
    16561 acttatctat cgtgggaatc cttattgctc atgattacat cgcttagttt gttcaaagcc
    16621 atgatgaatc tttatgtaaa atatgataat caagaacata ttctttcgac agtatccgga
    16681 ttgactttag tactgactat tttttggttt gttattgcta tttttattaa ggatatattg
    16741 gcagaaattt taggattgac accagttcta gttgtctgta tgttctcata ttttatattt
    16801 caggcagaaa tacaatgttg gtctttacac aaacgatata tatatgatta taagacttta
    16861 ttagtagcta cccttgcagt tactgttggc tcatctgtag ttggctgttt agcggttctg
    16921 tttatatctc ctaccgctga ggcgagagcc ttaatgaatg tcctagtgac tggtgttgtt
    16981 ggcgtggctg tgtatatatc ggtatttaga agatgtaagg attttaagga taatgaagtg
    17041 tggaatttcg cagtagtatt ttgtgtatcg ttgatgccac actatttatc tgaatttatt
    17101 ctgaatggtt ctgataaaat tatgataaat tacttatgtg gttcgtcaga tgttgctatt
    17161 tacagcgtgg catattctat tggtgctcta attaaccttg ttacaaattc tgtcaattcc
    17221 acttttgcac cttatcagta tcaaaaaatc aagtcaggag aatatgattt actatcaaaa
    17281 agagctaatg aagttcttgg tttggtagcg gctatgcttt ttgcattaat gctatttagt
    17341 aaagaaattg tgttgatatt tggtgggcag aaatatatcg acagtgttaa tttaattgta
    17401 cctatatgta tcggtattta tttcaactat atgtttcagc tgtttgctag agtacaggaa
    17461 tactttgaaa agaaactcac tgtcgttatt ccatcggttt tgtgtgcaat tatgaatatt
    17521 gtactaaatt atgtttttat aaagttatat gggtatcagg cggtagcata cacaactgta
    17581 ttctcatata tgtttttctg cgtcatccat tatatttttt atagaaaagt ttgcaaagaa
    17641 ctattggatg gaaaaaacat atatgatgtc agaggaatac ttttgatttc tgcaatcacg
    17701 atagctggtg gcatcctaat ttcaataatt aataaatttg atttcataaa atatttaatt
    17761 atagcaattg cttgtgtcgc tgcaattata tttagaaaac gattgctact tttagtaagg
    17821 agaattatga gcaaataaac atttgctcat agagagatag agaatgatag atattatttc
    17881 tgctataatg gtaaatgtat taacagcatt atatcagccg ttcttatttg ccgtattaac
    17941 agcgtatctg tttatgtact ggtatctatt tatagttgat aactctcgag atcctaaaaa
    18001 cttaaaagat tctgtattaa tctggttgag atattttaaa gcatctagta aatttcgcag
    18061 actctttttt cttgcgtttt attttacttt gatattattt aaaaccttgc ttaatcgaaa
    18121 tatgtggatg aacccgcttt caaacgttat tggacagtgg tcattatata ttatagataa
    18181 tgctacagga gagagcagat tgacaacaga atgcgtggag aacacgatgc tgtttatccc
    18241 tttttgcatg ttattgttat ggtggcgtga cattaaatgt gatattatac gcaccatcta
    18301 tgttgggatt aagtatgtgt ttttattctc attaagtata gaatttacac aactgttttt
    18361 taggcttggt acatttcaac tatcggattt gttttataac accctagggg gcttaattgg
    18421 tgctttgcta tattggctat tctaccgatt aaataagtat atagacttaa aatagaactg
    18481 ctttgaattt taatatggtt aatatttttc tgcagacagg attgtgtaat ctcatcaaga
    18541 atactttaca tgccatctat ttttgctaaa acattgttgt tatatcttca agaggtaggg
    18601 aattttattg ctttaaatga ggtcgagcga attaaaaaat tggtaaaata tataacatgg
    18661 tggtagttat ggtaaaagtg tttgatataa ttaagttttc ggatttcaga ggatatactg
    18721 ctgttgcatt ggataatgag actcttttct cgattggtat tgagtttcac attaagcaaa
    18781 taaatcaagg atttagtcat aataaatata ctatgcgagg gctacattat cagagtggag
    18841 agcatgctca aggaaagata gtgatgtgcc tacatggtga aattatgaat atcgcacttg
    18901 atattcgaca aggatcttcc acttatggat gtgtgtatgt caacacattg agtgaaagca
    18961 atggtaaatg tgcgtatatc ccacgtggat atgctcatgg ctatcttact atggaggaca
    19021 atacgctagt tcagtggtgc gtagataatg atttcaattc taatgctgcc aaatgtataa
    19081 gttatgaaag cgtagatatt gaatggccgg tcagtcggca gtgtttcgtt ataagtgaga
    19141 aagacaaaaa tggggaaaga ttaagctttt gattaaaatg agctgtggag aaaagaagga
    19201 tactggaaaa taagaagaac aataaccgag caaatgctcg gttatttcta tgaggaatac
    19261 ataaaactaa atttagatat agatatttat tcacgaaaaa ctaatatt
//
LOCUS       DACCCR010000052        19297 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCCR010000052 DACCCR010000000
VERSION     DACCCR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.059"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            236..1627
                     /locus_tag="IVK93_001824"
     CDS             236..1627
                     /locus_tag="IVK93_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6387112.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1686..2489
                     /locus_tag="IVK93_001825"
     CDS             1686..2489
                     /locus_tag="IVK93_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6387113.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2522..3376
                     /locus_tag="IVK93_001826"
     CDS             2522..3376
                     /locus_tag="IVK93_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAP6387114.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3373..4074
                     /locus_tag="IVK93_001827"
     CDS             3373..4074
                     /locus_tag="IVK93_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAP6387115.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4082..5149
                     /locus_tag="IVK93_001828"
     CDS             4082..5149
                     /locus_tag="IVK93_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP6387116.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5142..6107
                     /locus_tag="IVK93_001829"
     CDS             5142..6107
                     /locus_tag="IVK93_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6387117.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6152..7126
                     /locus_tag="IVK93_001830"
     CDS             6152..7126
                     /locus_tag="IVK93_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6387118.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            7197..8684
                     /locus_tag="IVK93_001831"
     CDS             7197..8684
                     /locus_tag="IVK93_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAP6387119.1"
                     /translation="MFIYILRIVIQFVGRSFFIYFLGAKYLGLNGLFTNILSFLSLAE
                     LGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIGIVGVLIIPLLPLFIKN
                     STDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIA
                     LYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIG
                     NMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGN
                     FAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILI
                     VLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIG
                     TIGSSLGFVIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANGG
                     STFLQFITKTLITIVTTSILYLLLYFRREEFTFIKKILFRYILKK"
     gene            8741..9856
                     /locus_tag="IVK93_001832"
     CDS             8741..9856
                     /locus_tag="IVK93_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6387120.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            9853..11268
                     /locus_tag="IVK93_001833"
     CDS             9853..11268
                     /locus_tag="IVK93_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP6387121.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIY
                     IVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNA
                     TKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNA
                     RITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGI
                     LGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTV
                     GTFVFWLFLGRVNSKNNSSYF"
     gene            11301..12248
                     /locus_tag="IVK93_001834"
     CDS             11301..12248
                     /locus_tag="IVK93_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP6387122.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(12288..13448)
                     /locus_tag="IVK93_001835"
     CDS             complement(12288..13448)
                     /locus_tag="IVK93_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6387123.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            13613..14788
                     /gene="mltG"
                     /locus_tag="IVK93_001836"
     CDS             13613..14788
                     /gene="mltG"
                     /locus_tag="IVK93_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP6387124.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            14924..15403
                     /gene="greA"
                     /locus_tag="IVK93_001837"
     CDS             14924..15403
                     /gene="greA"
                     /locus_tag="IVK93_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP6387125.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            15571..16302
                     /locus_tag="IVK93_001838"
     CDS             15571..16302
                     /locus_tag="IVK93_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP6387126.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            16299..17361
                     /locus_tag="IVK93_001839"
                     /pseudo
     CDS             16299..17361
                     /locus_tag="IVK93_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            17368..18000
                     /locus_tag="IVK93_001840"
     CDS             17368..18000
                     /locus_tag="IVK93_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6387127.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            18140..18805
                     /locus_tag="IVK93_001841"
     CDS             18140..18805
                     /locus_tag="IVK93_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP6387128.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            18833..19126
                     /locus_tag="IVK93_001842"
     CDS             18833..19126
                     /locus_tag="IVK93_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP6387129.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(19194..>19297)
                     /locus_tag="IVK93_001843"
     CDS             complement(19194..>19297)
                     /locus_tag="IVK93_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6387130.1"
                     /translation="FQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         6735 a         2702 c         3714 g         6146 t
ORIGIN      
        1 tatgaatatt gtaacttgtg aataaggaat tagcaggaga atactagttc agcataaagg
       61 aagtgatggg acttctgaga caactttctg ttatgcagaa atacacatat aaacagacaa
      121 taaacgcttt tccccgtttc ttttaatttc ctttacaatt tggttattca ctaggttttt
      181 aaactttgct atggtaagat gatagaggca ttattttatg gttaggagtg tatatatgaa
      241 taaaaatggg gagtggaatg cggcacgcag aatattcatt attcttatgg atgtacttgt
      301 attaaatgta tcgatctatc ttagtttttt gctgaaattc cagggagaag taccagtgag
      361 aaacttagaa acctttcagc attcagcaat ttttatttcc atcatcttta ttgcgttgaa
      421 tattttgtta ggagcttatg tattctataa ccgaatgatc agtgatattg tcttcattac
      481 tgttattgga caagtactaa tagcattggg gatcatggta gttacttttg ctggccgatg
      541 gtttgcattt ccgcgtggcg tcattttgat ctcgtttatc ctaggtacga tattacttag
      601 tgtttaccgg atagtcgttt acaaactgta cttaagagtc agtcgtgata agaaagtcct
      661 tatcgtgggt ttggagaaag atgtcgcacc agctatccaa aattttcaat ctaagaaaaa
      721 caataaacat aaggttgaag cagtggcaat cgataattat tacgaaaata caaaaaaatt
      781 gatcgatgat atggatatcg tctatttggc ttctaaaatc ccagaagatg aaaaaatcaa
      841 catttatcaa ttagtgacta aaaaagagaa gaaattattt ttaaatacga cttttgagaa
      901 tctgacaatg ataaatccga atattatgaa ttttgaagat gaaagcatca ttgaagcttc
      961 tgggttcaga atccaaccag aatacgaact attcaagcgt ctctttgata tcatcgtttc
     1021 gcttatttta ctgatcctcg cctcaccttt tatgctaatc acagcgattt tggtaaaaac
     1081 aacttcacct ggaccaatca tctataaaca agtacgtatt acgaaaaatc aaaaggaatt
     1141 ttcaatctat aaattccgtt ccatgagtgc gacagctgaa gcaaaatcag gaccagtctt
     1201 ggctaaaagt aacgatgcac gtgtgacacc agtaggtaaa tttatccgtg ctgtacgttt
     1261 tgacgaatta cctcaaatct ttaatgtatt gaaaggcgat atgtcgattg tcggtccaag
     1321 gccagaaaga ccatttttcg ttgatcagtt caatgaagaa aatccttatt attacttaag
     1381 acacaatgta cgtgcgggaa ttaccggata tgcacaagta tacggaaaat atgtatcaga
     1441 ttataacagt aaactacgtt ttgatttgtt gtatatcaaa aaatattcat tactattaga
     1501 tttgaagatt ttacttcaaa caatcaaaat cttatttgat aagatgtcat ctcaaggact
     1561 agatgaagat gaaaatatta gttcattagc agaaatcgac ttttcacaag agaaaattta
     1621 tcgctagatt ggaaaaattt tgagcaaaga ggtaggagag aacattggaa aataaaaatg
     1681 taagtatgct gctttcttta tatgataaag aaaagccaga gtatttaaag gaagcattaa
     1741 ctagtatttt tgagcaaaca gtaatgccaa aagaaattgt attagtttat gacggcccta
     1801 taaatgaaaa acttcaagta gttgtcactt atttccaaca aaaagatcct cacttgttta
     1861 cagttgtaaa attagaaaaa aatcaaggat tggggattgc tttagcaatt ggattagaac
     1921 atacgaaaaa tcgcttagta gccagaatgg atacagatga tataatggaa ccccaacgat
     1981 tagaaaaaca attagagatc ttccaaaaag atccagaaat agcgattgtt gggagtaata
     2041 ttgaggaatt tactggcacg cttagcaatg ttatcggaaa aagaattgta cctgaatata
     2101 acaatgcaat tcgtgtattt tctaaaagaa gaaatccctt taatcatatg accgttatgt
     2161 ttgataaaaa agctattttg gaagtgggaa attaccaacc attaagtggg tttgaagatt
     2221 attatctttg ggcaagatta ttaaaggcag gttataaagg atataatatt caagaaacat
     2281 tagtctatgc gcgaactggc aaagatatgt atgcaagaag aggcggaatg aaatatcttg
     2341 gttctggtct tatgggtcgt tatcgtatct ggaaagacgg tttagggtca gtaaaagatt
     2401 ttataattgt ttcttgcgga catattttta ttagcttact accaaatcaa ttaagaggaa
     2461 aattttatca aacgcaatta agaaaatagg aggtttttct ttgtatagca ctgatccaaa
     2521 aatgaaaaaa atacaacaga ttacgttgaa aatgtctcaa gagattgttg atttttgtct
     2581 aaaaaatcaa cttctttgtt atttctgcgg tggtggtgcc atcggtactg tgagagaaca
     2641 agggtttatt ccatgggatg atgatttaga tttctttatg cctagagagg attatgaaaa
     2701 attttatcaa ctatgggtta attcagaaaa aagtaaaaaa tatcccatac agaaggcttc
     2761 taaaaattat aatgatcata attcttttac aactatccgc gatgcagata ctacatttat
     2821 taaaacttat caaagagatt tagatatagt ccatggaatt actattgata ttttcccgtt
     2881 agatgttgcc cctgtttcga aatttagccg taaaattcaa aaactatggg ctttagtata
     2941 tgctttattt tgttctcaag tcgtccctga aaaacatggt ggaataatgg ctacaggtag
     3001 taagatactc ttatctattt tccgctcacc tcgtacgaga tataagatat ggtcttttgc
     3061 tgaaaaacaa atgacaaaat ataataatga accaacgggc tatgtgactg aactttgtgt
     3121 cggacctaaa tacatgggaa atgtttatcc tgcaaaagac tttgaatctg ctgtgtgggt
     3181 gccattcgaa gatacagaga tgccagtacc agttggttat gataattatt taagtcaagt
     3241 ttttggtgac tatatgcagc ttccgcctga aaaagatcag gtttctcatc atgaagcagt
     3301 ttatattgat ccagaacatc catacacaat gtacaaagga aaatattatt taactaaagg
     3361 agcagaaaaa aaatgattac agctattatt attgctggtg gtgtgggtaa acgaatggga
     3421 caagatatcc caaaacagtt cattacaatt gaaggaaaac ctattattat ctatacttta
     3481 gaatcatttc aacaccatcc tcaagttgac cggattttag tcgtttgcaa atctggttgg
     3541 gaagaaacga tgtgggcgta tatcaaagaa ttcaatatta gcaaagtcaa atgggtcatt
     3601 acaggtggat caaagggaca agagtcgatc aataatggtg ttcagttttt gaaagactat
     3661 tctgaagatg atgatacgat catcatccat gatggaatcc gtccactagt agacgaactg
     3721 gtattgtcag atgtaatcgt taaatgtaaa gaatttggta acggtgtaac ctctctacct
     3781 tacaacgaac aaatttttat caagaaaaca gaagaaacga cggaacagta tgtcgatcgt
     3841 aacacattga ggcgtgtctc aactccacaa gcttatcaat acggaaaact acgggcagca
     3901 tatgaccggg cagtgaaaga agacatcggt atgactgatt cttcttatac gaataccatg
     3961 atggtagatt tgggagaaac tttatatttt gctgctggtt ctgataaaaa tatcaaattg
     4021 acaacaacag atgatctaga attgttcaaa gcatatttaa aaatgaaaga ctaggtgaaa
     4081 gatgatgacg ttgattaaac aaccaatgta ccaagaagat ttggcaaaag taatcaatca
     4141 tacaccaaaa ataaaacagc tacatggaaa atccgttctg atgattggtg cttctggaat
     4201 gatcggctct tttttgattg atacgttgat ggctgctaat gaacaactag gcatcaaaat
     4261 caaagtttat gcaatgggca gaaaccgtca gaagcttgaa aaaaggtttg ccagttatct
     4321 tgaattagat actttcgaaa ttgttgaagg tgacgtcaca gaacccttac caaaagaaat
     4381 tcaagctgac tatttgatac acggtgcaag taacacacat cctaaggctt acgcaactga
     4441 tccaatcggt acgatcatga ctaatcttgc aggtacagag caagtattga aacatgcagt
     4501 agcaacccaa ccggttagag tattattttt atcaacagta gaaatctatg gcgaaaatcg
     4561 aggcgatata gaaaaattca cagaagatta ctgtggatac atcgattgta atacactgag
     4621 agcaggctac cctgaaggaa aaagagtcag tgaatcatta tgccaagcct acatcgcaaa
     4681 acacgacctc gacatcgtga ttccaaggct ttgccgtacg tttggtccga cgatgctttt
     4741 atctgatacg aaagcatcct cacaatttat tttaaatgca gtcgaacaaa aagatattgt
     4801 tttaaaaagt gaaggaaacc aatatttttc gtatgtatat gttggagatg ctgtttctgc
     4861 tattcttcat ttactgcttg aaggagaaaa aggagaggcg tacaacgttt cttcagaaaa
     4921 ctttgattta cgactaaaag aattagccca acagctagct gatatcagcg gcaaacaagt
     4981 aatttttgaa ttgccggatg agattgaaca aaaaggtttc tcaaaagcta gcacagcagt
     5041 acttgaaaat aaaaaaatta aagagacagg ttggaaacca atttttcctt tgaacgaatc
     5101 attggttcat acagtagaat tagttaaagg tgagaaatat aatgtgtgag attagtatta
     5161 ttgttcctgt gtataaagtt gaaaaatatt taagaaaatg tgtagattcg attttagccc
     5221 aaacatttac tgattttgaa gttatattag tagatgatgg atctcctgac aatagcggga
     5281 aaatctgtga tgagtatgct gaaaaagata atcgagtacg tgtgatccat aaagaaaacg
     5341 gcggattaag cagtgctaga aatgcgggga ttgatgttgc gagaggcaaa tacttaggct
     5401 ttgttgacag tgatgattat attgatgaag atatgtatga aattttgtat gagaatttga
     5461 aaattcatga tgccgacatt agctctgttg aattgattcc tttttatggt gatagatata
     5521 agaaagctaa taaagaaaag aaagtaatta tattaaataa aaaggaagca ataaaatctg
     5581 ttttagaagg aacgcaattt tatgcatacg catggaataa actttatcgt aaggaacttt
     5641 ttaaagataa tcgttacttg gatggaaaaa catttgagga tgcatatata ataatagatc
     5701 tattatttca aacagaaaaa attgttgttt ccaatgaaga gaaatatttc tatcttcaaa
     5761 gaaatgatag tattatggga aaaagttttt ctatgaataa tttcgatgtt atagaagcat
     5821 ggcaatataa caaagagaag attttagatg cttttccaga tttgcatgat tcctattatc
     5881 gccgactatg ttgggcatat ttttacgtgt tggataaaat ggttttatca agtagttatc
     5941 aacaaattcc acgtaaaaaa acagtaattg aatttttaaa gaagaacaga aattttatat
     6001 taacttataa gggattcact aaatcgcgca aaatagctat tattgctctg agctttagag
     6061 tagattggta taggaaattg gttgaacaaa aaaataaaaa aaattagaat gtttaaatta
     6121 gtaaataaaa gaaaaggaat tgaaaactaa aatgtgcaga ataagtataa tagttccagt
     6181 ttataatgtt gaaaaatatt taaaaaagtg tgtcgattca attctaggcc aaacatttac
     6241 cgattttgaa ttattattaa ttgatgatgg ttctacagat aattcaggaa gtatatgcga
     6301 tgaacttgcg aaaacagaca accgaataaa agtgatacat aaggaaaatg ggggtctaag
     6361 tgatgccaga aatattggta ttgaggtagc aaagggagat tttattgggt ttatagatag
     6421 tgatgattac attgatgaag atatgtatgc atttttatat aacaatatat tgaaatatga
     6481 tgcagagtta tcaatgtgtg gtatttatga cgtccataag aataaagaaa ttaaaaagtt
     6541 aactcctttt tctcaattgg ttactaaatc tgaagcaatt gaattagtat tagatggtaa
     6601 gctagttgtt gctaatgcag taagcaagct atataaaaaa gaattatttg agaatgtgag
     6661 atatccaaaa ggaaaaattg ctgaagacgc agcagtaata ctgaaaatta tcaatcagtg
     6721 taataaaatc catgttgata cttctcaaaa atattattat tatcatcgtg gagatagtat
     6781 tactagtaaa aaatttacaa aaaaagattt tgatataatt gatgtatgga atgagaatga
     6841 aaaatggatt attaaaaatt atcctcaaat gtacgataaa gtacatacta gagtttgttg
     6901 ggcttacttt gttgtgttag ataaattagt tttaactgaa gaaaaaggac tgtatcaaga
     6961 aaaaaaacaa gtaagagagt atttaattga taatttcttt tttattataa aaaataaacg
     7021 attaactttt caaagaaaaa tatctatgtt attgcttact tttggaattg ctgcgtataa
     7081 aataccggtt aaatatcagt ataagaaagt aaaggctgtt aattaatgct ttagttagga
     7141 tgaattagat gaaaaaaagc aggttagagt attctttaat aaattcttct atatcaatgt
     7201 ttatatatat tttaaggata gtaatacaat ttgtaggtag aagttttttt atttatttct
     7261 taggagcgaa atatttagga ttaaatggtt tatttacaaa tatacttagt ttcttgtcgt
     7321 tggctgaact aggcataggc tcttctatta tttattccct atacaaacca ttagcccaaa
     7381 aggatgaaag taaagtaaag gctttaatga atttgtataa gaagacatat gaatttattg
     7441 gtatttttat cggcatagta ggagtattga ttattccgct gttacctcta tttattaaaa
     7501 atagcacaga cataccacat atgtatcgat attatgtatt atttttatta aattcagttc
     7561 ttagttattt tttcacgtat aaaagatcac ttgcaatagc agatcaaaaa aattatctgg
     7621 ttgcgattaa tgattttgcc tttttattct ttatgaacat tgtacaagta attgctttgt
     7681 acttttattc taattttact ctatttctaa tagtgcagat tttgtttact ctattttcta
     7741 atataaatat ttccagaaca gttgacaaga gatattcata tttaaaagag aaagatgtag
     7801 tttctctaga taagcaaaca aaagatgaag taaagaaaaa tgtcataggt aatatgtcct
     7861 caaaaatagg cggggttatt gtaatgggga cagataatat attaatttct acatttgtta
     7921 atttaacagc tgtaggaatt tattctaact attcattgat tgtcacaagt gtgcaaaatt
     7981 tatgtaagca aattactaat tctatcacgg caagtgtagg aaattttgct gtatcaaata
     8041 aaaaaaagga ttcatttgag ctatttaaaa aacatttttt tatcaaccac tcactaatct
     8101 ttttttcttc tatattgtta ttgactttaa tgaatccctt cattagcttg tgggtaggag
     8161 ccaaatacat gctacctatg tcaacgacta ttttaattgt tttaaattat agtgtacagg
     8221 tttatagaaa tacatccttc gtttttatag agtcatttgg tttatattgg tctcaacgga
     8281 aaaagcctat aattgaagca ggtgtaaact tgttattatc tttgcttttg ttaatagttt
     8341 ttgatatggg aatcaatgga gtacttatag ggactatcgg atcgtctcta ggatttgtga
     8401 tttggtatga agcatttatc atatataaaa cggtatttga gaaacgtttt attcatttct
     8461 ttttaacttt ctggcgaagt tttatagaat taataatttc ggcaatagtc gtttttctat
     8521 taaatgaata tttagcaaat ggaggcagta cgttccttca atttataact aaaaccttaa
     8581 taactatagt aacaacatcg atattatatt tgttattata ttttagaaga gaagaattta
     8641 catttatcaa aaaaattctt tttaggtata ttttaaaaaa atagctttct ataaaggagt
     8701 gttttatttg aaaatagcta tactatctcc agctttctca atgggaggag ctagtatcgt
     8761 ggcctcaaaa attgcaaaag gattaataga tagtggccat gaagtttttt ttgtttctta
     8821 cttagaaagt gaaaaaccac aagtagaatc taagtattat gatttatacg aaaaaaaaat
     8881 ttttttattt aaaaattttt ctcgatttca taagatatta gagtatagat tgtttggagg
     8941 ctttgttcct agtagatata taaaaaaaga aaaagaacaa ctaaaaaaag ttctaaagaa
     9001 agaaaaacct gatgtcatca tattcaatac ttttatacct agtgtactat tctccgaatt
     9061 cctaaagtat gaatttccaa cagtgaaatt aattacttgg atgcactctg atcctgatta
     9121 cagcttaaat catattgcta aattttataa aaaagaatat agaaaaagtt ttgaacgcgt
     9181 ggattccatt gtttgtcttt cgcaagatac aaaagaaaaa ctttcattat acaataacca
     9241 tattaaagtt atttataacc cattgacatt aagcggctct agtttaataa gtaaccttga
     9301 taaaaaaatt atttcattta ctgccagata cgatataaat attaaagggt ttgattattt
     9361 atgtgaagta gctaaaaact tacctgttgg atgggctata aggattgctg gagatggtac
     9421 agaagaagaa cgtaaagagc tgaaaagatt aattgagaaa aatggtgtgt tagataaaat
     9481 tatttgcgca ggtgcattat ttggagatga attaatcaat cattatatag aaagttctat
     9541 tttcatatct acttctagaa gtgagggact accattagtg ttgatagaag caatgactta
     9601 tggattacct gtaataagtt ttgatcatgg tggtgctaag gaaatattac aagatggaaa
     9661 ttatggaatt ttaattaaga attttgacgt acaagatatg aataatgaga tagaaaaatt
     9721 gtctaataat cgtttactta tggaaaaata tcaaaaattc tctttagaaa gagctaagga
     9781 ttttaatgaa aaaaaaataa ttggtgaatg gcaaacattg ttaaatgaat taggaaatga
     9841 ggatgttaga aattgaggac ttcgattaaa aattttaatt tatggaaaga atggtatcta
     9901 tatatatttt ttatagggat gattattcgt gcaataaatt tttatagttt attaccgtcc
     9961 acttttgata gtattttttt taaacttata ggaatttcag gagttttgct attagggata
    10021 gatttcataa ttaaatttaa taaaagaaaa gtaacttata acattttatt gatagcatat
    10081 atggtgattt tagtaataac gagttttgtt cataaagaat atggtctagg agaaaactta
    10141 aagactataa tgtgggcaat cttacagtat tttttgattt atcagtttgc ttttgagaat
    10201 agcgataata aacgattttt taaaaggata agttggggat ttatttatag ttggtttgtt
    10261 ttagtatcaa tgtcaatggt tcttttcttt ggtaaattcg gctatgagaa atattataat
    10321 gctagacata ggatcagaat tggtttttta gaatcaagac tgtttgggac gttttctgat
    10381 attaacaatg ctgctatagc atctttagtt gttattgtcc taatcttatt ttatctttat
    10441 aataaaaaag tgacaggatt aaataaaatc atttttttaa ttaatcttcc attacaattt
    10501 atttatatag tattaagtgg gtcacgatct acctatgtgg taagtataat aatagttgcc
    10561 atatcatctt ttattgttgt gttaaaacga aatggaacaa aaaaagattt aaaaaactat
    10621 atccttgcta tagtatcttc tattggttct gttctgattt tattcgcttt gtttaatgca
    10681 actaagatac tttttcaaga attattacct catttaaaag aaataaattt ttttaatttt
    10741 acagagcgtc ataaagaatt aatgaataat agtcaacata ttaataatac tcaaacacct
    10801 gataatactc ttgttaggaa agacgtagct gaaaatacag atatttctaa tgctagaatt
    10861 actatatgga aaagtgcatg ggaaatattt aaaacaagtt ggcttatcgg agtctctccg
    10921 aaaaatgtag tatcttatgc acagtcaatt attccagatg gatatattgc gaaagtagga
    10981 attgccattc ataatgcata cttgaatgta ttaacatcga caggtattct aggaataatt
    11041 cctttcatgg cttttttaat aaagagtttt gtgctgaatg tttatcgaat catctataat
    11101 aaatgcattg gtaaagatta tcattttgtt tactgtttag ttattttatc ttatgcacta
    11161 tacgcagctc ttaacaatga attagtctat gaaaacactg taggaacgtt tgttttttgg
    11221 ctatttttag gaagagttaa tagcaaaaat aattctagct atttttagta ataaatttta
    11281 tcttgaaaag aggagaaaaa atgttgaatt acttgattac cggtggcgcc ggcttcattg
    11341 gctcgacgct cgcgaattat ctaggaaaag aaaacaatgt agttgtgatc gatgatttat
    11401 cgatggggaa aaaagagaat ctggatgaat cgaagcacat cacgtttatt gaaggggatg
    11461 tcgctgacaa aaatttgatg gaaaaggtca tgaaagaata ccatttcgat tatattttcc
    11521 atctagctgc tgtagctagt gtggcagatt ctgtagaacg tcctctagaa actcatcgtg
    11581 taaattttga cagtgcgctt ttattgcttg aattggtacg aaaatatcaa tctggtttaa
    11641 aacgattggt atttagttca tcagctgcag tttatggcga tgaaccaacg ttgccaaaaa
    11701 aagaagaatc ggtcatccgt cctttaacac catatgccat cgataagttt gcagcagaac
    11761 aatatgtttt ggattactgt catttatatg atgttccgac tagtgctgtc cgatttttta
    11821 acgtttatgg tccaaatcaa aatcctaact ctccatatag cggagttatt tcgattctag
    11881 tggaccgata caaaaaacaa ttggcaggag aaaaaacaga atttacttta tttggtgatg
    11941 gtagccaatc tagagacttc gtttatatag aagatgttat acaagcactt ttacttgttg
    12001 ctaaggaaga aaaagcttta ggacagcaat tcaatgtcgg tacaggaaaa agcactacgc
    12061 tacttgaatt gattcattca attgatcaaa tcttaggaac agagctagcg cttaaatatg
    12121 aagcggagcg ttcaggggat attcgtgatt ctttagcgga tatttcaaag atccgttctt
    12181 taggttatca acccaaattt gatatattga acggaatgga acgttattta aagacagaaa
    12241 tcgattgaaa caaaaaacca gcatcctgct ggtttttttg tctcgactta gctttttaaa
    12301 ttaacacctt ccatcagttc gtaaatctga tttcttaaat cagtggccgg caatttgatg
    12361 tttttatcag attggatatt actttccatg acgattgcat cttttgtgtc ttttttcatc
    12421 gccgctactg catggaatcg attgatattt ccctgagcaa ggatataatc atttccactg
    12481 taaacaccgc cgctgtaagg atgtttatca ccattatttt gccacaatga gaaaagtagt
    12541 tcttttggta taatatcgcc agacagaaca ctggtaaaga atttgttcaa gtcataaaca
    12601 gacatactta tatttcctgt gcctaattca tttcggatgt ctgtttcagg ctcgaccaat
    12661 gcttttgaat aatcgtcagt tgcagacatt tggtaagata cagcatgctg aggggaattt
    12721 tttacatttt cataaaaatc tgtatgttct agatggagtg gccgaatgat ttcttttttt
    12781 aaaagagttt cataaggctg attcattagt ttgatcaata cccctgtaag caatgtataa
    12841 tttaatggtt cataatgata cgtatcataa ccagtaaatt tcaaatgatg aagggcaaac
    12901 tgaatgatct cactatctga cataggtttt tttggagaag cggttcgatg aagaccagag
    12961 cgcatgtaaa gcatattttt gattgagatt tgctgacttc caggaatctg aggataaaat
    13021 tcagatagct ttgtgtcaaa agttatcttg ccagattgaa tttgttttag gataagtaaa
    13081 gcagtcatcg ctttttggat cgaaccgatt tgatagtaaa gagaaggaga attcgattct
    13141 tttatgcgta gatttgcgta cccatatcct ttttctaata tcactcgatt atttttgcgt
    13201 acataaatcg aaccgatata atctgatttt tgcagtaatt gatcaatttt gctggcaagc
    13261 atctgctcat tagatgttaa attggaagca agttgtgcgg gttccgtttt ataaatttgt
    13321 ggttgaggtt gttttgttaa ttgccatcct gtaatgatgc ctgttgaaaa gaagctgaca
    13381 gccagcaaca aaactgcgag aaaagaaaga gagatctgct gttttttttg atgttttcct
    13441 tttgccatat taaatccttc ctatccattt ttaactattt ttcattgtaa tcctcaaaaa
    13501 gagcatttgc cacttacaat tttgacataa tatttggtaa aataattcac ggcagtagaa
    13561 atttcatgta taataaacag agttacgaag atgatagtaa ggaggagttg ttttgtcagg
    13621 aaacaaacaa cacccaagca aagacaaaaa agaagccagt aaggcagcta cagcagatat
    13681 aatcgacaag cggagaaatc tgcgtaaaaa agaagataag atcgtgagga agatcatttt
    13741 agtgatcgct cttacattat tgattattgg cggatttctc gggtttaccg tatatcgtta
    13801 tgtagatagc gggttgaagc ctttagataa atcggacgac caattagtcc aagtggaaat
    13861 cccaagcggc tcttctaata agcagattgg cgaaattttg gagaaagaca atattattaa
    13921 aagtgggatt gttttcaatt attatacaaa atttaaaaac ttaactggct ttcaagctgg
    13981 ttattatcag ttagcgccta atatgactct tgatgagatt ggcaagcagt tgcaagaagg
    14041 cggcacatct gagccaacaa aagttgcaga tgggaaaatt gccattccag aaggctacga
    14101 catcgatcag attgctgagc gggtagcgaa ggtaactggt aaagacaaga aagaattttt
    14161 agatttagta aatgatgaga ctttctttaa cgagttgtat caaaaattcc cagaattgtt
    14221 ggaaagtgcc tctaaagctg agaacacaaa ataccgtttg gaaggctact tattcccagc
    14281 tacttatgat tactacacga gtacttcgct taaggatctt gtgattgaga tggtgaacaa
    14341 aacaaatacg gtcatgcaaa actattattc agctatcaaa cagaaaaatt tgactgtaca
    14401 agaagtttta actttggctt ctctagttga aaaagaaggc gtaaaagaaa atgatcgtaa
    14461 aaatattgca caagtcttct ttaaccgaat caaggcaaat atgccacttc agtcagatat
    14521 ctctgtttta tatgcattag gagaacataa agaattggtg acgtatgaag atacagctgt
    14581 agattcacca tacaatttgt ataccaatac cggctatggg cctggtcctt ttgacaatcc
    14641 aagcgaagag gcaatcaagg cagtattaga accagcagaa aatgactatt attatttcgt
    14701 agcagatacg agcactggaa atgtttattt cgctaaaacg tatgaagaac atatggaact
    14761 agtgcaaaaa tatgtaaata attcttagaa aaacaggggc tagtcattga tcatttttag
    14821 aaaaacattt acattttaag aaaagcgtgg taatcttttt tggactatat tcctcaaaag
    14881 attacctttt gcggtatttt atcgaaaaaa ggagacgtta cacatggtag agaaagttta
    14941 tccaatgact ctagaaggta aagaaaaatt agaaaaagaa ttagaagaac taaaaacagt
    15001 taaacgcgga gaaatcattg aacgaatcaa aatcgctcgc ggtttcggag atctttccga
    15061 aaactccgaa tacgaatcag ctaaagatga acaagcattt gttgaaggaa gaatcacaac
    15121 aattgaaaat atgatccgtt ttgcacaaat catcgataat gacggcgtag atgcagatga
    15181 ggtatcgatc ggtaaaacag ttacgttcat cgagttgcct gatggagatg aagaagagta
    15241 cacgattgtc ggaagtgctg aagcagaccc gttttctgga aagatttcca atgattctcc
    15301 tatcgcgcaa gcattgattg gcaaacgtaa aaatgatgaa gtaaccattg ctacaccagg
    15361 tggcgatatg ttagttaaaa tcgtcaaagt agaacaagcg tagtctgact gaaagatcta
    15421 gagagttcat cttactgatg acgcactcta gatcttttct ctttgagtat tgcaggtttc
    15481 actgaacgat cttacatcta aagtcctata gccaacagtc aaaaaaatag gtatgatgaa
    15541 atgagaatgt gtcaggaggg agaaaaagcg atgaatagtt cttggcgttt ttttgtggta
    15601 gtcgaagcac tgctgctgtt atttgctgtt tggcaaatcg taaacaatac tggacttttg
    15661 cttttagtaa tattcggtat tttcaatata tatctagcta tgcgcaaata tcctcgtact
    15721 aaatttcaaa attttcaatt gatcttaggg agcttggtca ttttttttag cttagtcaac
    15781 agtcctgctt tatggatgat ggttgtattg gccgttttat ttatcgggct gaaaggtgta
    15841 gagatttcag gaatagattt gacaaaaaat gctttttggc gaaaaaaaca gatcatgatg
    15901 gtccaaacag agcaattaaa aacacataat aatgagcgta aaagacagca attatttggt
    15961 aaccaacgta ttggaaacga cgtctatgaa tgggatgata tcaacattgc catcatttct
    16021 ggtgatacta tcatcgattt gggcaataca cttttgccaa aagatgataa tattgtgatt
    16081 gtaagaaaag ggataggcag aacacggatc ttagtacccc tgggagtggc tattcgttta
    16141 gagcatgcga cattagtagg aaatgtcttg tttgaagaag agcagttttc cttaaaaaac
    16201 gaacagatca aaatctgcag cagtgattat gatgaaaatc ctcgcagatt gaagatcatt
    16261 accaatactt tggttggaga tgtcgaggtg atacgaatat gatgggaaga atatccaaag
    16321 caatgctagc tgtttattcg gggattgcgg ctttccttat tattctattt tcacttttca
    16381 cttattttta tgccagtaac caaagtcatt ggtggggaga attgctgcgt gcgcgtttat
    16441 tatacgttcc gcttatcttc catttgatgg caatttcatt aggtgtagga ttgatcgtat
    16501 ttctattgtt atcacttatt caaaaggcaa aatacgggaa aatcgaagaa aagcttcgtg
    16561 cactttcttc tggcaattat gaatccaaac ttttgcatca gccgatccca agcgcgtcgg
    16621 atgatctgta catcaaagat attgatatgg aaattactaa aataaaagaa aaaatgatcg
    16681 aagtatccag tgaattacag attatgacta gccgtcctca atatgtagat ggacaaacca
    16741 aagaagagat tttaaaatta gaaaggcatc gattagctcg tgagcttcat gattcagttt
    16801 cacagcaatt atttgcagcg atgatgatga tgtcggcatt gacggagcaa gcagaaaaaa
    16861 gtgagacacc agaaatgttc cgtaagcagt tgaaaatggt ggcagagatc atcaacgctt
    16921 cccagtctga gatgcgtgcg ctgcttcttc atctgcgtcc agtcaattta gaagaaaaaa
    16981 gtttaaaaca aggcattgag cagttattga aggaattgca gaataaaatc cagatttcgc
    17041 taaaatggga ggtagaagat gtaaaattaa ctagttccat tgaggatcac ttgttccgaa
    17101 tcgttcaaga attgttatca aacacattaa gacatgctaa ggctaatgaa ttagaagtct
    17161 atttgcatag ataacaatct tttattacgt atcattgacg acgggacagg atttaatatg
    17221 aatgaaacca aaacgggaag ttatggattg aacaatatca aagaacgagt agctggtatt
    17281 ggtggaacag taaaaatcat tagttttaaa gggcaaggta cgagcgtgga gaccaaggtc
    17341 cctttgatga aggaggcata gataaagatg ataaaagttt tattagtaga tgaccatgaa
    17401 atggtacgct taggcgtctc ttcttattta tctatccaag aagacattga agtgattgga
    17461 gaagctgaaa atggacgtca aggctatgaa aaggcgatgg cacttcgacc ggatgtcatt
    17521 ttaatggatc tagtcatgga agaaatggat ggcatcgaat caacaaaagc aatcttaaaa
    17581 gattggccaa aagccaaaat cattatcgtc acaagtttta ttgatgatga aaaagtttat
    17641 cctgcaattg aagcaggagc agcaggatat ttgttgaaga cgtctacagc acatgaaata
    17701 gctgatgcta taagggctac gcaacgcggc gagcgagtac tagaacctga agtgacaaca
    17761 aaaatgatgg aaaaaatgag tcggcgaaat gatccagtat tgcatgaaga attaacgaat
    17821 cgagaaaatg aaattttgat gctgatttct gaagggaaaa gtaatcagga aatcgcggat
    17881 gaattgttta tcacactgaa aacagtcaaa acgcatgtgt cgaatatatt agctaagtta
    17941 gaggtagaag accgaactca agcagctatc tatgcgttta aacatggttt ggtgaaataa
    18001 aaacgatgat aaagaggata atcaaaagct gagaaaataa ctattatttt tcttggcttt
    18061 tttacaataa tgatgcttgc ttttgttttt ggaaaggctt tgttatactg agaaaagaag
    18121 atactaaagg aggaaaatgg tgaaacaaaa ttttgcaatt gtaggccttg gacgatttgg
    18181 cggaagcatc tgccgtacac tgatcgaagc tggacaagaa gtactggcta ttgacagtaa
    18241 tgaagatcga gtcaatgaat atatgaatat agccacccat gctgttgtgg caaatgcaca
    18301 ggatgagatg acacttcgtt cattaggtat aagaaatttc gatcatgtaa ttgtcgctat
    18361 tggagaagat atccaagcca gcatcttagt tactctgatg gtaaaggaaa tgggcgtacc
    18421 gaatatttta gcaaaagccc aaaatgagta ccatgcccgt gtactagaga agatcggagc
    18481 agatcgtgtc gtacaccctg agcgagacat ggggatacgg attgcacata acttagtatc
    18541 aaagaatatt ctagactact tagagctgtc agataaatat tctttagcag aaatccgagt
    18601 ctccaatcct aaatttttca acaaaacatt agctgaatta aatttcaggc aacgttttga
    18661 cttaacagta gtcgccatac gaagagtaga tcaaacggtt atcgcttctc ctgcagcaga
    18721 tgaatatgtt cgtgaaaatg ataatctact ggttattgga gagacaggcg atgtggatat
    18781 tctagacgat aagatgaatc aatagaaaga aaagagaaag taggttgttg ggatgaaatt
    18841 aacggtaacg ccaaaagcac aaaagtggtt tgactcagaa attgatctgc cagaaactta
    18901 cggaatacgt ttttttggaa aagtctacgg caaaacagag gttcatgatg gtttttcgat
    18961 tggaatgtca gttgaacagc cagaacatcc tgtaaagaaa gtggaaatgg atgggacact
    19021 tttttttatc gaggaagcag atgaatggtt ctttaaagga tatgatttac ttgttgatta
    19081 tgatcccaac ctggaggagc caacctatca ctttaaaaaa caataaaaaa agagagggtg
    19141 acacaatttt tgtcaccttc tcttttttct tttgaaaata ttgttttcag ctattaatat
    19201 cctgcagaca attttacttg attggtttga agctcatggg tggcaaagta gcttttcaga
    19261 ttggttaaaa aaatagccat taatttcttt tgaaaat
//
LOCUS       DACCCR010000053        19240 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCCR010000053 DACCCR010000000
VERSION     DACCCR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.060"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            169..363
                     /locus_tag="IVK93_001844"
     CDS             169..363
                     /locus_tag="IVK93_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387131.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            632..931
                     /locus_tag="IVK93_001845"
     CDS             632..931
                     /locus_tag="IVK93_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387132.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1028..1462)
                     /locus_tag="IVK93_001846"
     CDS             complement(1028..1462)
                     /locus_tag="IVK93_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6387133.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1694..2404)
                     /locus_tag="IVK93_001847"
     CDS             complement(1694..2404)
                     /locus_tag="IVK93_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6387134.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2394..3254)
                     /locus_tag="IVK93_001848"
     CDS             complement(2394..3254)
                     /locus_tag="IVK93_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP6387135.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3259..3897)
                     /locus_tag="IVK93_001849"
     CDS             complement(3259..3897)
                     /locus_tag="IVK93_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP6387136.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3912..4211)
                     /locus_tag="IVK93_001850"
     CDS             complement(3912..4211)
                     /locus_tag="IVK93_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6387137.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4254..5738)
                     /locus_tag="IVK93_001851"
     CDS             complement(4254..5738)
                     /locus_tag="IVK93_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6387138.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5758..6219)
                     /locus_tag="IVK93_001852"
     CDS             complement(5758..6219)
                     /locus_tag="IVK93_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6387139.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6237..7304)
                     /gene="ulaG"
                     /locus_tag="IVK93_001853"
     CDS             complement(6237..7304)
                     /gene="ulaG"
                     /locus_tag="IVK93_001853"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP6387140.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7522..8292
                     /locus_tag="IVK93_001854"
     CDS             7522..8292
                     /locus_tag="IVK93_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6387141.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8565..9392)
                     /locus_tag="IVK93_001855"
     CDS             complement(8565..9392)
                     /locus_tag="IVK93_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387142.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9960..11195
                     /locus_tag="IVK93_001856"
     CDS             9960..11195
                     /locus_tag="IVK93_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP6387143.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12194..12454)
                     /locus_tag="IVK93_001857"
     CDS             complement(12194..12454)
                     /locus_tag="IVK93_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6387144.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12481..13782)
                     /locus_tag="IVK93_001858"
     CDS             complement(12481..13782)
                     /locus_tag="IVK93_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6387145.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14074..14532)
                     /locus_tag="IVK93_001859"
     CDS             complement(14074..14532)
                     /locus_tag="IVK93_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6387146.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14545..15870)
                     /locus_tag="IVK93_001860"
     CDS             complement(14545..15870)
                     /locus_tag="IVK93_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP6387147.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15887..17299)
                     /locus_tag="IVK93_001861"
     CDS             complement(15887..17299)
                     /locus_tag="IVK93_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6387148.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17316..18080)
                     /locus_tag="IVK93_001862"
     CDS             complement(17316..18080)
                     /locus_tag="IVK93_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP6387149.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18184..19059)
                     /locus_tag="IVK93_001863"
     CDS             complement(18184..19059)
                     /locus_tag="IVK93_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6387150.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6118 a         3954 c         2951 g         6217 t
ORIGIN      
        1 ttaagaacaa tagcctttta ttatatatat tattcaaatt agttatatcg agtgaaaaaa
       61 actttcaaat tgattctttt tatttatcat aaagatatag atacataatc attttcactt
      121 acatatattt gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta
      181 gaagtaaaaa caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat
      241 cctttacttg gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata
      301 aagaacgagc tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag
      361 taaatccaat gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat
      421 tatagaatta gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata
      481 ccatatgatt atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat
      541 gaatcaaata tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt
      601 ataatgtatc agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga
      661 actacttaag caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa
      721 agtgacagag tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc
      781 aaaaggaata tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc
      841 tttaaaaaaa aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt
      901 tgataaagag gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata
      961 ctaagtctat cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta
     1021 ttatatgtta tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag
     1081 aaataccacc catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt
     1141 tctcagtatc atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca
     1201 aagcttcatt caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga
     1261 tatcttgaat aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct
     1321 tcccttcccc attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg
     1381 cagtgtcttt tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga
     1441 tcataaggga atcaatattc atttttttct tcctttctta tacaattctc cctctaataa
     1501 tacagttctt tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt
     1561 ccttaaaatt cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat
     1621 aaattttctt cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct
     1681 taccctagca tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt
     1741 tatctagtaa atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg
     1801 ccatcagaca aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc
     1861 cccatgtgaa cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt
     1921 ttctttcctt aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta
     1981 cttcttcttt cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag
     2041 tcgtgccata agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat
     2101 gagtatgagt aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga
     2161 gatctgaaga tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca
     2221 ccatttgatc tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc
     2281 ctagctcacg gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg
     2341 gcaatgccag atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag
     2401 acatcatagc cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct
     2461 cttcgaaagt cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct
     2521 agacgtttca aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca
     2581 tcacgaaact ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca
     2641 gtgatataat cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac
     2701 aaattgccta ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct
     2761 aaaaattttg aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg
     2821 ccatatttag aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa
     2881 ttcattgatt tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt
     2941 agattatgcg ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct
     3001 ggatcagcag aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt
     3061 acaccggagc tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat
     3121 cgtgctaacc gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct
     3181 tttacaaggg aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc
     3241 ccaatttgag ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc
     3301 ggccttcatc ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc
     3361 gatcccttca aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa
     3421 gcctagttca atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga
     3481 taacagcgca tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc
     3541 catggcttgt tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt
     3601 ttgtgctgcc tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc
     3661 tgcttttgca acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac
     3721 tagtttttta tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa
     3781 gcaaagaatt gtgcccactt caataatatc tactacatca ccaactttca taacatctcc
     3841 taatgcatgt tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt
     3901 agttccttcc tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt
     3961 tctttgactg acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga
     4021 gtaaacatat tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt
     4081 tttccctctg aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt
     4141 gctttttcca cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact
     4201 aaaattctca tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact
     4261 ctctgctttt ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt
     4321 ttccgatatt gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca
     4381 taacctagga ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc
     4441 ccgatataac caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct
     4501 acttgtatga ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta
     4561 ttggcataaa ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg
     4621 atcgttggtg atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca
     4681 aacaagaacc caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca
     4741 attcctggaa cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt
     4801 tcatcgacga atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa
     4861 gaagtcgtca taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc
     4921 aaataatctt tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa
     4981 gtagcaacca tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt
     5041 tttttgtctt gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct
     5101 aattttgcaa atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct
     5161 tctgtcaatt cctgcgtgat tcctactgtt aagttagaag ctactgccca ataacaacct
     5221 aaaatcagtc ccataaaaat caatacttgt atcctaccta gatcaggaaa gcaaaacagt
     5281 aaaatccaaa aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa
     5341 cgcagtttcg tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt
     5401 aataaaatca tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca
     5461 gcattttgtc caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt
     5521 ccaaccaaga ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag
     5581 taaccgaccg ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa
     5641 atatagccta ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt
     5701 gcaaaatacg tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta
     5761 attatattct gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc
     5821 aaccaatcca tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc
     5881 tggatctttt gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc
     5941 actttcaaaa cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc
     6001 atcacttttt tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc
     6061 gtatttttct acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc
     6121 gatcaataca agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg
     6181 gtaatgaatc aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat
     6241 aaaaacgacg gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa
     6301 aaattaaact caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag
     6361 atataaggtt taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc
     6421 tcttttggat cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt
     6481 gcattcaatg actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct
     6541 cgtgggtttt caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg
     6601 gcaaataaat tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg
     6661 aataaatagt ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct
     6721 tctggatctt cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg
     6781 atgtctttga ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt
     6841 ccccattttt gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa
     6901 ttttgatgaa ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg
     6961 attaaagcgt ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa
     7021 ttaggctgca tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg
     7081 ccattcccat gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct
     7141 tcatgacttt tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga
     7201 tccactactt ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct
     7261 actaaccact tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca
     7321 tatcgttgtt ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt
     7381 tatatgaccg tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata
     7441 ataaagacag atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat
     7501 gtccctcagg aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa
     7561 gaaattttgg aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc
     7621 cttgatgtat ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag
     7681 atcatacgca attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa
     7741 tctggtcctg aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat
     7801 tatgatacgc tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa
     7861 tctaaccatt tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat
     7921 aattgtagtt atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc
     7981 gacatcgcat taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc
     8041 ggagtcgatg tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa
     8101 atgatggtca acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga
     8161 ctgacatcaa agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa
     8221 ttttgttctg aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta
     8281 gattcatttt aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac
     8341 aaatttatcg ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt
     8401 atccatccaa tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct
     8461 aaataggaat caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt
     8521 cgaataaacg gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt
     8581 tcaatcacct ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata
     8641 tatctttcta aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc
     8701 aagtattcca atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat
     8761 aattggattg ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat
     8821 acaatgcaga ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc
     8881 acataaactt tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca
     8941 gctgcttgta agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa
     9001 aataatgctg catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa
     9061 atttgattta aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta
     9121 taagattctt ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg
     9181 attttgtctt cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa
     9241 agcgcactta agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt
     9301 gcttgttcac aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat
     9361 tcttttagta atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat
     9421 ttctccttta tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa
     9481 taaaaatttt aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat
     9541 ttctttttgt gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc
     9601 atggcgagaa agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa
     9661 tgacgacagc acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta
     9721 tttttaatga cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt
     9781 gttgcgtgtt tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt
     9841 tgtgaaaaca gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt
     9901 gttttttagt atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca
     9961 tgaagacaat tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg
    10021 aggttgccaa agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt
    10081 ttgctccttt gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga
    10141 aaaaacaaga agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta
    10201 ccaaagaaat ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc
    10261 ctcgactgat tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga
    10321 ttcctttagt atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg
    10381 cgctgatgcc ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga
    10441 caaaaaagcg tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa
    10501 aagaaatgct tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca
    10561 tcacaacgcc taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg
    10621 gtccaatttt tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc
    10681 gaaagcaaca agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc
    10741 gtatgttacg caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac
    10801 ctattgacta tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat
    10861 atctgtctag tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag
    10921 ggaccgtcca aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg
    10981 agcaaagctg taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac
    11041 cagtgactga aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc
    11101 agcaggcacg cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag
    11161 aaatcgaaac cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa
    11221 tagagactca tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta
    11281 aatagtggat tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat
    11341 ccttaaaata aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc
    11401 ttagtcaaat tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa
    11461 agaccataat aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat
    11521 cgctttgtag agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt
    11581 taccacttta tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc
    11641 gatgcatact cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa
    11701 tgttctatgt cgtcagcaac gatacactaa aagtcacgtt caccgtgact gattgctatt
    11761 cccagcacaa ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata
    11821 ttaccattca tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata
    11881 tttgacttga tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt
    11941 ccattttttc ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa
    12001 aagggctgtg acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc
    12061 ttgcatctgc ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt
    12121 aagaagcaat tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc
    12181 acttttagaa catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat
    12241 tgatagcaaa aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg
    12301 gtacaatgat ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat
    12361 tgatttgaat atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa
    12421 agataaaaac aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac
    12481 ttagacagtc tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc
    12541 ataagctttt acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa
    12601 gataacaaca cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt
    12661 ccatgttggc aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat
    12721 aatagtcaaa actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat
    12781 aggagctcca aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg
    12841 accaacctct ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat
    12901 cgttgctcct gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata
    12961 gggtaattgg gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat
    13021 cagccacatt ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca
    13081 caacaaatgg atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt
    13141 ggttaaaggc tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg
    13201 aatcagccaa gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc
    13261 aacagcaggt ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg
    13321 atatacttcc actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa
    13381 agaactcata ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg
    13441 aacttgtaac ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc
    13501 atatgattta gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat
    13561 cgtcatccta aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc
    13621 aggaaacggt aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt
    13681 tgaaacaacc ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg
    13741 tgggacaagt ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca
    13801 ataaaatatg aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa
    13861 ttaagaaatt ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg
    13921 aaaataagtt ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa
    13981 atgattaaat ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata
    14041 aatatcaccc tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat
    14101 ctttacttct tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat
    14161 gatcaccgta tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt
    14221 cccttctttt acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc
    14281 aactgtatct aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat
    14341 ggcatgtcgg gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc
    14401 atcatgattc gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt
    14461 tactgctgtt aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt
    14521 aaagaaatgc actttttatc acctctactt taatgctggc caatattttt tgtttacttt
    14581 gatcatttca tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt
    14641 aaatgcttgt aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc
    14701 tactgcaact tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt
    14761 aattggctcc acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg
    14821 tagattcgga atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt
    14881 caacaaggaa atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa
    14941 tacttcttcc acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg
    15001 tccatccatg accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc
    15061 aggatataag taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat
    15121 gactgacatc atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac
    15181 ttgcatttct tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc
    15241 tttatcataa atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca
    15301 attcttacga tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc
    15361 acaggcatta atcattttta cattcggata tttacgacga acagcttcag aaacgatcga
    15421 ctctggattt gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac
    15481 aatttccaat aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt
    15541 ttcttgtcct actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa
    15601 tcctccaaca cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc
    15661 atccgttgca cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat
    15721 caagtacata tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg
    15781 caaccgtttt tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc
    15841 tccagctata gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata
    15901 atttattgaa ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt
    15961 tttcagttgg tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca
    16021 tggtgggatc cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat
    16081 tggcttttcc tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg
    16141 ctaaccctaa ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac
    16201 gtcctaatgt ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg
    16261 caaaaaagaa aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga
    16321 tgagataatc aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac
    16381 agacaatccc tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa
    16441 acatgaaact aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc
    16501 ttcccatgat tcctttgacc atctctttat tttctttata agctgcttta tagatagcta
    16561 gacatatagc tggtatacca aacatcatta ctacaaaaaa acctgataaa aacagtccag
    16621 cctgaggatc tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag
    16681 atgtatagct tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg
    16741 ggatcagcaa acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga
    16801 gcagttttgc aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa
    16861 cagctgcgat tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat
    16921 aagaaaaaac tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta
    16981 aaccggaaat aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc
    17041 cttttttcaa ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat
    17101 ttttgacaaa accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg
    17161 catttccagc tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga
    17221 tcaatcccgc taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca
    17281 catttgattt aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac
    17341 tcaatcaaag gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact
    17401 tggtgggtac gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct
    17461 gcgtgacaca tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa
    17521 tattcttcca cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg
    17581 aaacaaggct caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga
    17641 accggcaaat catatttttt tgctaatgta acaactacct cctgattttc atccaaagta
    17701 tgagtatgat gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga
    17761 atctgagcat tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa
    17821 taagataagg atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt
    17881 ttgccacacg tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt
    17941 ttcacagcat gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct
    18001 cgctgataag catccataat cccataattc acaccatggc tatagccaaa atcatccgaa
    18061 ttaaaaatca cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca
    18121 aataatcata gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt
    18181 attttaatag attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc
    18241 aagttgaaat tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct
    18301 atgtttaaat gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat
    18361 caagcgttta accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt
    18421 atcataaacg tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac
    18481 tatttttaag ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt
    18541 gatgaatcct acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac
    18601 cacttctact gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat
    18661 tgctgtgaca tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa
    18721 tgtaatatag atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac
    18781 ttcttccgca aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt
    18841 gatcttttct ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt
    18901 actcacttta gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc
    18961 atcgatcaat acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc
    19021 tctatatgat aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca
    19081 atttttaaag acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa
    19141 tagtagcacc ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata
    19201 tcttttttac tcgttttatg ataatacttg tatcgaattg
//
LOCUS       DACCCR010000054        18895 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCCR010000054 DACCCR010000000
VERSION     DACCCR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.061"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(21..251)
                     /locus_tag="IVK93_001864"
     CDS             complement(21..251)
                     /locus_tag="IVK93_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387151.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IVK93_001865"
     CDS             complement(687..1253)
                     /locus_tag="IVK93_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6387152.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IVK93_001866"
     CDS             complement(1253..2209)
                     /locus_tag="IVK93_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP6387153.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IVK93_001867"
     CDS             complement(2209..2865)
                     /locus_tag="IVK93_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP6387154.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IVK93_001868"
     CDS             3235..5253
                     /locus_tag="IVK93_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6387155.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IVK93_001869"
     CDS             5680..8094
                     /locus_tag="IVK93_001869"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP6387156.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IVK93_001870"
     CDS             8442..9995
                     /locus_tag="IVK93_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP6387157.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IVK93_001871"
     CDS             complement(10376..12160)
                     /locus_tag="IVK93_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387158.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IVK93_001872"
     CDS             complement(12175..13896)
                     /locus_tag="IVK93_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387159.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IVK93_001873"
     CDS             13996..14757
                     /locus_tag="IVK93_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6387160.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IVK93_001874"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IVK93_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18895
                     /locus_tag="IVK93_001875"
     CDS             16664..>18895
                     /locus_tag="IVK93_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387161.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3353 c         3786 g         5551 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DACCCR010000055        18708 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCCR010000055 DACCCR010000000
VERSION     DACCCR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.062"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(93..1004)
                     /locus_tag="IVK93_001876"
     CDS             complement(93..1004)
                     /locus_tag="IVK93_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6387162.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1381..2019)
                     /locus_tag="IVK93_001877"
     CDS             complement(1381..2019)
                     /locus_tag="IVK93_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387163.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2023..2793)
                     /locus_tag="IVK93_001878"
     CDS             complement(2023..2793)
                     /locus_tag="IVK93_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP6387164.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(3211..3627)
                     /locus_tag="IVK93_001879"
     CDS             complement(3211..3627)
                     /locus_tag="IVK93_001879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387165.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(3678..4059)
                     /locus_tag="IVK93_001880"
                     /pseudo
     CDS             complement(3678..4059)
                     /locus_tag="IVK93_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4185..4850
                     /locus_tag="IVK93_001881"
     CDS             4185..4850
                     /locus_tag="IVK93_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6387166.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5247..7280)
                     /gene="brxL"
                     /locus_tag="IVK93_001882"
     CDS             complement(5247..7280)
                     /gene="brxL"
                     /locus_tag="IVK93_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HAP6387167.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7398..9920)
                     /gene="pglZ"
                     /locus_tag="IVK93_001883"
     CDS             complement(7398..9920)
                     /gene="pglZ"
                     /locus_tag="IVK93_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HAP6387168.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10036..13668)
                     /gene="pglX"
                     /locus_tag="IVK93_001884"
     CDS             complement(10036..13668)
                     /gene="pglX"
                     /locus_tag="IVK93_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HAP6387169.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13732..17403)
                     /gene="brxC"
                     /locus_tag="IVK93_001885"
     CDS             complement(13732..17403)
                     /gene="brxC"
                     /locus_tag="IVK93_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HAP6387170.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17428..18003)
                     /locus_tag="IVK93_001886"
     CDS             complement(17428..18003)
                     /locus_tag="IVK93_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HAP6387171.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(18003..18602)
                     /locus_tag="IVK93_001887"
     CDS             complement(18003..18602)
                     /locus_tag="IVK93_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAP6387172.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5769 a         3566 c         2619 g         6754 t
ORIGIN      
        1 ataattaaac tactgttgat ttagttctaa taaaaagatt tagatgaaca gttttattga
       61 tttaaatagt tcatattttt tatttatacc gcttatttac tacttgctgt actagaatct
      121 gaagtgaatg tactcaaaac attttcaaag tctttgtctt tgatttttac attagcgtct
      181 ttcaatactt cgccaattac tttattctgg aaggtactgt cacttaattt tgtgtctgtt
      241 gcaatttctt tcaattcttt tttgtattta tccatgtcgt tgcctttgtt ttggtttttc
      301 accattttaa ccacataata ttccgtagta taagttgaag cattggtaga agtgatcaca
      361 tcagaaactt gaccatcttt caacttaaag gctgcttctt tgacttctgc cggaaccgtt
      421 gtagtagtgg aatcaaattt cacttttccg ccatcttctt ttgtcgtagt atcagttgac
      481 ttgtctttgg ctaatttaga gaaatcgtct cccttatcag cagctttctt cacatctttt
      541 gcatcatctt cacttgctac ttggataatt tgagcttcca cttctggatg gaaagattcc
      601 caagccgttt ttaaatcttc atcggtcaat ttgatatgtt tctttagacc ttcttgaaag
      661 gctagacttt gtttgatact gtctttgaat gtttgttctg tgtaacctgc agatttcaat
      721 tgtgaatcaa atgaatcacc caattgtttc tttgtttgat catattggga atcgatttct
      781 ttggttgata cgtcatctcc atatttatct tcaaatactt gataaacgat caactgttgc
      841 acagcggaat ctgctccttg catttttttc aagctatcca ttacttcttg ttcgctgatt
      901 gtaccgcctt tataactaac ggctgttttt tcaccggcat tcgtacagcc tgacaataag
      961 ccagtgccta gtacgatggc aagtgctaaa aatctttttt tcatttatca attctcctac
     1021 ttttcgtttt ttgcgaattg ctaaaaacaa aggtgttcaa gatgaaaatc atcaagaaca
     1081 cctttactaa tctgctcgat acgtcctgaa tagatataag gctacacaat ccagaaagtt
     1141 cgctcagcag aataaatgtc aagtagcaca tcacacctta atatactact tcttttgtta
     1201 ccgtttttca agtctatctt gcgttccgta tttaacgtaa ctaattctta gaacaaatgt
     1261 tatcgatttt gtttttcttc ttccgctgtt gggatattca aatcagacac ttttatgttc
     1321 gattttccag acattttttt gttgttttta ttagaaaaag tttgtttttt catttgtttt
     1381 ctaaagcatg atcttcttgt ttcctatttt ggtcagatat ggatcatgcg tgaccacgat
     1441 aactaacgta ccttgggaag cctgttgcga taagatttca acgacttgct ccttatttcg
     1501 tttatctaat gatccagttg gttcatcggc taaaatgatt ttgctattct tcaataagac
     1561 tctcgcacag gcgattcttt gcttctcacc accagataat gtgtagatag gcgtacttag
     1621 cttcttgttt atatttactt ttttcaatgc ctcatacttt gctgttcgct tatctgaaac
     1681 atttaaattc gaaaacttca atccaatatc taaattaaaa tcgattgttt catattcaat
     1741 caacccaaaa tcttgaaaaa gaaaagagag ggcatttctt tttatcaagc gcttctctct
     1801 tccagttaaa tgttttgttt cttgattaaa taaataaaat tgccctgaac tgaattgatc
     1861 attcaatcct aatatattca ataaagtact tttccctgct ccgctctctc caactacagt
     1921 cacaaaatcc ccagcatccg cagtgaaacg atccaaatga agaatcgttt tgtttcgaat
     1981 gatttttttc gcacctttta tcactatcaa cggctccaat tgttaacctc cattcatcag
     2041 caataactga cttttctttt ctgtagacaa aataaaaaca actgaaagaa taaaatcaat
     2101 gaagctaaag aaaaaaatat taaaaagata tttaggctct ctttctataa gaaaaaggaa
     2161 gattacactt gtcgttcccc atttgatgag gttcatcatg aagaaatctt tatacgtttc
     2221 aaagagcgaa taaccacttg aacgaaagat agcgattttc cgacaattgt agacaaaata
     2281 tatgcataac atgacatagc tcaacaagaa gaaagaaaag aaagtgagta aaaatatctg
     2341 tatcaagtaa tgaaatgatc tgcttaacac tttgacagct atctcttcag tctgagtata
     2401 cggaaatgat aactgcaagt tatcacgtaa ctgactttct tcaatcagct caaataacct
     2461 ttctttcgac aaagagccta tcttgatctt taacggtgga tagatttctc cgctgagaat
     2521 atttctttcc caatacgtac tgtttttcaa tgtcagtatt tcaacaaccg gataatgttc
     2581 gatccagggg atagaaggaa taaatgtata aatgggttga ttgtttttta taaatagaaa
     2641 tttcggttct ttttcactga tttcttgata atattctgtt atttgcgcca ggtcattttt
     2701 taaatctgta tgtctatatc gttcagggat caatataaca gggtgcgttt ctttttcggt
     2761 tatttggatc aaccttccag agacatcaat caaaggaaat ttcttaagat aattcagatt
     2821 tatttttatc cctctgccaa actgttcatt ttcttcattc aagtaatctg atacattgac
     2881 taatatactt ccgtttttat tcaaagtttc atataattcc tgatcggctg ctgatgcaaa
     2941 acgattggaa taaataaaat ccacgtggtt tttcccgatg gttactggat aaaatacatg
     3001 atagtcctca ccagcgattt cttttggtgt ttcatgggaa acagctaact gtgaagaaga
     3061 aaaaacgata gttgctaatt cgacattggc aacggtcaaa agaagagaaa acaacaatcc
     3121 aaatgtaaaa agaatgtatt ggatatagtt gaacgcttct cttaacgaag cgattttttc
     3181 aactaataaa agagaaatca aagagatacc ctatattccg ctgaacaaga aaacgtacat
     3241 ctggaacaaa taaagaaatt gctccttata atacataaaa ataaaaccac caatgattcc
     3301 tgcaattatg aatgtaataa tgattttctt tttccccaat aaacaccaca atacttgatg
     3361 taatgaaaag ccattattca acaataaact gatttcttta ttcatcaata agaaaaagga
     3421 tgataacaaa aggaataaaa gaatcaatcc aatagttaaa tacactcgat aattatatac
     3481 gtctgtactt gaaataaccg aatacgaata gctattttca aagtcttcaa aaatattagt
     3541 ggcataatca gtgtgaaatg cttgattgaa tcgttccaca aatagtaaaa tgaaccgttc
     3601 ataggcttct ttttctctcg tcatcaagaa aaattcacct tcaaaatctt ttattttgat
     3661 cgattcttca atggacgtta cgcgaaagga ggtattagaa tagggtacct tctcgtgaga
     3721 agatttgaca tccccttgat aatacaatgt catttcaagc tttggctgta tcttgaagtt
     3781 ccaagatgta tctacttcat tggattcatt tactgttttt ttcatataag aaagctcagt
     3841 ttctgatgag acattttcaa ataccatgat tagctgctca tattcttctt gcccaataaa
     3901 atgttgtggc aatcgaatcg gagtataagt atctgtttga tggaaaaatt catttgtccg
     3961 atagagtacc tgtttattta taataaccaa ggcaaaacca ctcatgatac cggtcactat
     4021 gattgtgagt aaaactgtga actttttttt gattatcacc actctccctt acttaaatga
     4081 ttctttgata aaatcatacc gtttttcatc aaaaaaacgt atctatcaaa tgtataggtc
     4141 atgtagaata aatctatata ctgtagaatc gaaaggagaa gaagatgaac atcggagaaa
     4201 aaatacgaac ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc gaaaaaatcg
     4261 gtgtttccga taaaacaatc tctagttggg aaaattctcg tacaatgcca gaccttgaaa
     4321 tgctgtcact tttacatcag actttaggtc tgccgattga ttttccttct attgaatcag
     4381 ctaccgaatc aatagaacca acattgccgg atgaacctct tgttatcaag aaatcttatt
     4441 catcaaaaca acccttctca ctcaaaaaga cactatcttt ctttctgctc gtcttttgtt
     4501 tggtctatct ttttcttatc aattatcaat ataaaaatga aacggttgat cgattcaatc
     4561 cttttttgaa accacaaatc ggctatacca cactccctgt aggatacgtg gaaaattcca
     4621 tcgaacattg ggtatgtaat gatatctttg ttcatggtac atggctgtct tttcacgctg
     4681 gagaggtaga aaaaggtaag aatcatgctg taatcaaaca caaaggaaaa cacgtcatga
     4741 acgttcgcct gctcacttgg gaagccattc cctcagatta ccaagaaatc ctcatccaag
     4801 actatgacga tgccttaaaa agaagccctc aggagccaga taggcattaa gtaggagatg
     4861 aacgtagaga tagaaaaaat caatgcacat gatcttatca aaaaaaaaca ggggtacaaa
     4921 ttaattgaaa aagctttgag atgaaagttc ctaaataaaa aagttcagaa tgataggtaa
     4981 aaaaatgact aactattcta gtattaggct atttgtatta ttcttctgtt atttttatat
     5041 caaaaaatcc agaaataact aaatataggt atttctggct tatagtataa tatttacgcc
     5101 acagccccca atgattcttt ttggaatcat gatcaaggac ggacatacac aaaaatattt
     5161 tgcccattcg atccgaatag attaacgact aatctagtaa gtaatataga ttggtacgta
     5221 gtcaaaccag caaatggaaa agttgtctat tccaccccaa gggctttaaa gacagcatct
     5281 tccggcgttt gataaaaaat caaattaaag gtacccatta attctggggg tacagttcca
     5341 atatcagcag cgcttatcat aggtacaagt acttttttcg ctccggcatc ttttgctact
     5401 tgtaaacagt tagctaaatt atctacctta atcatagaac caccaattgt aatatcccct
     5461 aaaataacta aacttggtaa tacaggttta tttaaggaaa ctgagcataa agctattata
     5521 gttgataacg ataatgtttt tgtcatacca atgccattca tatcttgaac atgaacgaca
     5581 aaatcttttg tcgtcaaact aatagactga ctaacacttc cataattagc tcttaaatat
     5641 cggtatgccg aatccatcgc ttctcttgct ccagcatctg aattgatccc agtacgttca
     5701 aactttccat ttcccgaagt catttgcgtt tccaatttat aggtacctaa ttgcccagtt
     5761 ttgccataag ataccgtgta caaatgccct ggcttcccta acccttctgg aatcaaagtt
     5821 cctccacctt gttcaggaac agaaacatac tcttctcgct cttcttctaa atcaatgtat
     5881 gaaaaattga catcataaaa ttccattcca ccaattttct ttaattgttc ttttacacga
     5941 cgacgcattt caagagcaaa tcttagtatt tcttcgacat cacttttttc aaatctacca
     6001 tcaggataca acagtttcat catcccagat acaatccgac gaactgcaat cgtatcccgc
     6061 tgatttaatt gagaaccaag tttgaaatat ttatcaaaac tatcgccaaa actaactttt
     6121 cgaagctcac gcatgacttc agaaaaataa tctgtaatga agccatagtc atttgtaaaa
     6181 aaatctggtc gatatttagg aatttcccaa cctggtaaat aacaatgcat ccgatccaaa
     6241 aaggctgaat ccgtccccat aacctccgga aatggctcaa ataaattcga agttttcaat
     6301 attacatcaa cgctttgatt aatatttcct acaaaagcca ttgatgctga agcattcttt
     6361 tcttctttgc ctctagcaaa agatcctgaa gccatgtaat ctttcatgat ttgaacgcca
     6421 tcattatcct taaatttaat cccagcaact tcgtcaaaag caacacagtc ccacatacct
     6481 acaagtccca ttgtacgact agccatatta tagaaaaggt ttgctactgt tgtttgtcca
     6541 cctgaaacaa ggattgagtt tggtgaaatt tctttatata catgcgattt acctgtacca
     6601 cgtggtccta gttcacagag attaaaatta ttttcaatta atggaataag tcttgctaaa
     6661 tgaagccatt ttaccctttt attaaattgt gtaggttcca taccaatgga ccgtagaagt
     6721 aagtcaatcc attcatttgt agtaaactcc ttgcgagcca tcttgtaagc ctcaatgtct
     6781 aaaccaggca tttggattgg atcaaccttt tttgcaataa aagggctatt ccaacgatca
     6841 ttctcatcat accaatattc catcgaaaca atacaccaga tccccccacc taataatcga
     6901 tcaaattttt tcggatactc ttcactaatt tgaacatttt ttaatcccag gttagagaat
     6961 tctgcttcat aacaatcttt cttttcattt aaatgtacag ttactttatc aataactgta
     7021 taaccaccca attcacgaat ttttgctttt actttttctg cttcatcagg acgcacatag
     7081 tttcgagcaa gaatattttt aacattttcc acaccttttt caaccaatgt ttcatccaat
     7141 gacgaagcat acataccaag taaatattcc aatacataaa caggtacatt cgcaccttct
     7201 ttaattttct ttgttaaatc ttttctgaca atctttccgg gaaagaattc agataacttt
     7261 aaaatttcct gttgttccac tagaattcct ccatgtgttc agtcattata atagcaatta
     7321 aagtatatca tacttaacca gtaggcttga taatacaaaa gaaaaagtat ccacaaaaaa
     7381 ttgtggatac ctcgttttta tatatcaaaa tcaaatgatt ctgcaaaagg attatctata
     7441 atgaatttta ttcgctcggt caaatcattt ttttgatttt ttatcactaa ataataagta
     7501 tcttcaactg tatatcgacg atttacaaat tcaaaaacaa atttagtaaa ccgctgattt
     7561 gcatcttctg tttttgaatc cgcaacatag atttcttcat ttgagatacg ttcaccatat
     7621 tcatcttcaa aataaagcca gaaagatgcg gggtcataaa catcactaat tgcatttttt
     7681 tgatagaatt ctaacgtggt tgataatcct gtaattctgc ggttgctagt cattaattca
     7741 attgctactg gttcctttgc attgctacct ttggctaccg taatttctag aacagggact
     7801 agcatttctt gaatagatga ccctccatga acgtagcttt gtcccccgcc agctttctta
     7861 aaaatacttg aagtaatcgg gacacaaatg tttctagtgt catcatttcc taaagaatct
     7921 cccaaagagt aatttttcgt accaattatt tcgtattctt cttcactaag aataaaacga
     7981 cgttctacac gatctttttc atttctagat ggattttcaa tcttatctac ttcttcaacg
     8041 tggcttcttt tataaataaa tccatgatca gccgtaacta taaaacgata gatactgcca
     8101 tcattatgca agcgattaat aagtcgggaa atttcgctta tcgctaaatc acaggcctca
     8161 aaaactcgat tttcattcga gtgatcacct gttctatcca cttgatcgtg ataaatataa
     8221 atgactgatt tgccattaaa tacttctcgt acctcagcct tattcatctt aaccacatca
     8281 tcataattta aagctacagc atttgcatct cgttgctgga gaatcgcatt tcgattggat
     8341 aaaccattcg tttttttccc atcaatcagt acttctgttc cttcattgta agttagttga
     8401 ttgtttggta ataaggcggc tttaccgaat tcagtaactg aaggtaatac agaaagaaag
     8461 ctagccatgt ttgttgaata cttcttttct ctaactaact tacgttctaa attcatccct
     8521 aactcatatc gaaaagcatc tgaaataata actacagttt taacagagcg tttcattaca
     8581 gcttgattat aaaattttcg aatggatgga cgattcttaa attcaaaagt ttgattccaa
     8641 atttgtcctg attgatcaag aaacttcgaa taatttgctt caactaatcg atacaattgg
     8701 ccatattttt gcttttcttc tatatgatca ctattccaaa taaatttccg ataatgcgta
     8761 tcaacccaat acatttcctt ctcatattga gttagcaaat tatccatgtt catcggcaag
     8821 cacaatctgt ttttatccac aagataataa gcatgtttca ataattgata ctcataaata
     8881 taatccgatg cgaaatgcat tttttcgcgc tgctttaaac tatcaaaaat tgtttgtcct
     8941 gcaattttcg ttgtagtgtc agaagccttt aatcgttctg cataaaacat taatattttt
     9001 gcatgaatct cagaaaatac gtctgcttca attagatcat caatttctat actgtctaat
     9061 aatccatcac catcaatagc ctgatatact tttttagata gctgttcaaa gctttccatg
     9121 taacgtgtat cattcataaa ctgatccatg aaagtaataa tgtttgcttt caaatgtagt
     9181 atttcaaatc ctttgaactt cttcggcatc tcttgatcaa tttgataata aaaatagttc
     9241 aaataaagac acatcgtcag cttttccaat gttggctggc tatccgtaaa gccaaatttc
     9301 atttgaataa attcccaaaa tttagctgtt aactgatact tatcaagttc ccttaaagga
     9361 ttctccctac tttctgtaaa gctttttaga actacacgca gtaaatcatt taatcgaata
     9421 cttttcgatt tcaacaaaac agccattatc attaattcaa tcgtttctga tttattaatt
     9481 gtcatatgcg tactaaattc accaaaactt ctcatgcgat cctttgcatt aaagaaatta
     9541 cgatactgtt taactgtctg atgaaatcta agtggaatag ccaattcatt catgataatc
     9601 gctaagcggt ctgcactaaa ttcctctgaa taattcaata cactcaacaa atgattatct
     9661 tcatctttat catcccaacg aggaaatggc gcatagatta aatagttagt ttcgggatct
     9721 tcatattcca ataaacgctt agttttaaat tgtgcgtcct tttctaatcg atagattttt
     9781 gcattttgaa tgaccaatga gtcaatttct tcaagaaaat cctgatttga atcaaaccaa
     9841 aaaatgattt tcctacatcc ataattagga aaacgttgat ttaatttttc ttcaatttgt
     9901 tgcgtattta tatctgtcat caatctcacc taatcgttct ctttctatac cccttatatt
     9961 gacataattt ggataatttc gcacgcaatt taagaataaa aaagaaagtt gttagaaaac
    10021 aactttctta gataactatt tttttggttc tttgacccct tttgcaaaaa tttggaattt
    10081 ctcgccggtt ttaggatcac gctgcaactt atcataattg acaattacac catcatctaa
    10141 gtctaattca attcgtttta atgctaagtg acccaacttc tcgtcatact cacgaacttc
    10201 ttttaattgc ttctgaatct ttacaatttc ttttgtcatt gcatttcttt cttttgggtt
    10261 atcacttgtt gaaatttgtt gctgacgcaa ttcgattcga ttgtcatacg ctttttgtag
    10321 ttcgtgtagg tattcagtac gtacttttcc taattgatct ggtgtatagc gatgcaagta
    10381 cattaacgct ttaaagccat tttgttttcc tgagtccaat tgccaataaa ttggtcgatt
    10441 ggagtatgtt tttaaatggt ctttgtaaaa atcttgtacg aagtaacgac gaataatttc
    10501 tcgacttgtc cctttgccac ctaaagtgtc agcgataaac tggagatttt cttcaagggt
    10561 ttctttacca aaagtaatct caacgaaatc aatgaaacga ttgacaatgt caatttggct
    10621 atccttgaaa tactcatcat cggtgatgag taaaacatta tcagtatcag gtttaaattt
    10681 ttgatagcgg gaaggatccc attcgccgcc agcgaagatg agaccatctt cgtctaggga
    10741 gtaacgaccg aagatacaac ctattgaata agaaataaaa tccttaattt ccctcgcctt
    10801 atcaggtaac cttgcagaga tatattcatc ttgaacctct tttgttaatt ctttttcaag
    10861 agaataatga ttaatgaata tgtcgttcag ttcctcttca ttcgatttta gagtattaaa
    10921 ttgattaacc aactgcctac ttagacagtt aaatgcctct gagagatctt ttttaaacat
    10981 ataacttaac aaaatgtttc ttctaaactc gtatgacgtt tcaagagcat tatagtcttt
    11041 tctagctata tcgatatttt catccacttt ttctcttatt ttttttaatt ttctctcaga
    11101 tgtaacttta agaaaaggta gttttcccat tggtccttca tggtaatcca aggtgggaga
    11161 aataaaagtt aaaaagtgat taactaattt agagtttaca tagccaaata tttcttcaaa
    11221 gtcagttata tcctttgaga acagaacaga tcctttagtt tcaaaaattg cctgattctg
    11281 caatctaaaa gatacttgtc cagatgaaag tgtcgaccaa gtaattccct ttttaaaata
    11341 gtaattaaga ttttgtggtc tagattttaa cttcccattt tcattaacat aattttttat
    11401 ttcatctcca tcattctccc agttaaccac gtattcattg tttccatacc attttctaaa
    11461 acgaccacct ttagttgtag gaaaccattt ttttctagac tttttagcgt cctctaatgt
    11521 tttagcatca aactttattt tgttaaattc tacttcaaac caataccgca aaaatttatt
    11581 gttatttccg gttgccaacc cttgttttgc atctgcaaca tcgttagtta attttccttg
    11641 agcaaaatca gtgattacgt tatcgcttgc ccagtaagca atcggcattc ctgggatttt
    11701 tttgaagttg tcttgattag tactataaaa ataatcgcat tcaccatgct gatgaattgc
    11761 ttctaacgtt ttaatttttt gaacttccat tccaccttta aaatctgata attttatata
    11821 gcttccatct tgttcatttg aattttttat cgtaaacgta caaattggaa cggttgcttc
    11881 ttcaaaagct gaatactcca tttgaatcaa actagaaatg tttctttcat taattaaaaa
    11941 tgagcgtaac ttttcatagc tagaaatgaa catccaaaca aatggcgtca taaaggaagc
    12001 atatccatta tcaatcagca tttggctatt caatttaata aacacagaaa atagatcttt
    12061 tttgacatca gaatagttat catttacata tttttttaat ttcttatcca ttttattaag
    12121 ataaggcgga ttagtgacta ccgctacata tttatcaact agcatttcag ccacgtcaag
    12181 aatgtgcaag tatttttctt gtatttcctt catcccataa agaatatcca tattcgtaaa
    12241 ttcgttgaat tttgatatat actctcttac ttttcctaag tattctttag ttattgcctg
    12301 attattttta tcgactaact taatgattga tccaagttct ttcccatttt caaattgatc
    12361 aaaaattaac tctttgtatt cattccagcg ttcactatac ttatcattgt tcaattcact
    12421 aatacgctct tgatactcgt ctgtcacttt aggagaatca ataaactctc ttaaattcgg
    12481 cgatagactt tttttcaaaa aaagacgatc gtattgacgc cctttcatca taattgcaaa
    12541 ataagacaat tgatacgctc ttttatcgat ttcaagacca tataaattgt attcaacgat
    12601 tgcacgtgca gcatcacgct cagaatatcc taacgtaaga taaatttcca tcaatacatc
    12661 gaaggcgtaa actaaaatat gcccagaccc cattgcgtta tcgataattt ttagttcttc
    12721 tggttttaaa ttacttacag atgttgcttg ttcaaaagca tatttatctg gaattaagta
    12781 tttcaactta tttcttaatt cactattggg ataatgatct aaccaatact ttcctaaact
    12841 attgtcaacc atataacgaa cgacccaatc tgtggtaaat agttgagttg ctgccggaat
    12901 atcttgtttt ttgacaatcc ctttataaat attcaccact tgattatgtg gctcactatt
    12961 atagtactga tacaaccacc caataatctc tacctgtcca ccagcttgaa catagaaatc
    13021 atcctctgga atacttaaaa gatcagcgat tacaccatct tttttaatat aagaagctgt
    13081 aaacaaaagt tctgtataat cttctacttc ttcaaatagc tcaggtaagt aatcattcaa
    13141 ttgattacag atttttataa aaatcaattg atacagcttg tccattgctt ctgctgaatt
    13201 atctgttttc caagttctga cttgttgctg ttcattacta gataattctt caaagatatc
    13261 ggcatcataa atatctgtaa taatatctgg ctcaattttc ccacttattt cactagataa
    13321 tacacgatac gtgtcaggta aataatcatt tacctccata aaccgaatgg caatcaatcg
    13381 gttaaaccac gtataagcaa cctcttcgat taactcttga aaagcttctg aataagaagt
    13441 gacagaagca cgttgtttta gttctgcaat caatttgttt ctttgcgcta tttcttttcc
    13501 ttgaattctt attcctgatt tttcgatttc ttgtaaattg ctatcaattt tcttcacatc
    13561 ttcaattttt gattcatcaa ttcctaaacg ttttgcttta gtcattactt catcaattaa
    13621 ttttctacgg gcattgaccg cgaaattctt tatcgctttc ttatccattt ttctttccct
    13681 tctatatgta acctttgaaa agacaggcac ctttttatgc ctgtcttttc actagaattg
    13741 aatcgtgatt tctgtatttt cttgaagttg ttccttcagt ttttgattaa tttcagctgt
    13801 taactcatca atatctgctg ctgacttaat agttttaaca tgaacgttaa gtgttctcat
    13861 tggtactgtt ttctgttgaa tagtaggttt tactatcgct atttcttcag cttttgctgc
    13921 agagtctatt tcatcttttt cagtttcacg aatcaagttt gctgcttcaa tttgtttacg
    13981 tgcttgttct tcttgacgtc tgcggttttc ttcattatcg aaacgttgat taaagatcaa
    14041 atactgttct gctacgttta atgataagaa aacaagatca tctatagtat tcgcttgttc
    14101 aatttttgct ttcagttctt gaaattcttg gtcaactatt ggtacaaatt gatccttaaa
    14161 atcttttcct tctaataatt taaatgttgc ttgtttttct tccgttaatt tatttaatat
    14221 ttcctgactt tccaactcta acagttgatt atattgttca ataaattctt gaatcaactc
    14281 atttagtttc atcatatctg aataaggatc agcctttttc aaaatggttt tcatctcatg
    14341 aatcaattga tcaatttctt ggctagaagt ttgacgccga cttttttcga ttaatctaat
    14401 tttatcttgt gcattctccc agattttttt ctgagtacta ttttgcttgt ggaaattttt
    14461 aattttatca tatgtttcag cccagtcgaa taattcatcg cgatattttt tgattgtata
    14521 aaaaaagtct tcaacatctg acgtttgaac aattacttca agatattttt ttatttgctc
    14581 atattcataa gcactagggt aggtataagt gttttgtaaa tcaaaataag agcgtaaatt
    14641 gtctaattga ccttttaaat atttcttgaa attttccatt gtatgttcat tgttattttc
    14701 atcaacaata ttacgacttt gaaacagttc tctaccgatt tcgtcaacca atttttttgc
    14761 ttttggcgaa atcaccgcgc ttggttcaat ttttatcttt tcactttctc gtcgtttttg
    14821 aataatcatt acaatactat ttggatcatc catccgtgaa atttgtacat tgttataaag
    14881 taaatcaatt ttaccagaca tgaaaagttt agcgatgcac cattccacat caagttctgt
    14941 gaatccatac ggttgttttg tttcaaaacg atcacgaatt gttttcatcg taatcgttag
    15001 gtgatttctc ttttgatcat tgataaaatt gaaaatttcc tgaactgctt gctcatttga
    15061 tggatcttca ataatttcat ttttgctttc tttcactaaa tataagatat cttctttttc
    15121 tttaggcgca tcaatttggt ttaatttatg gtatacttct tcagccattt tctgaagtgc
    15181 tgattggaat tttgctttaa agtcggagcc tgtcagttga atttctgcac cgttatagaa
    15241 aaattttgca gatttcaaca gttcatttaa ttgatcgttg atacttgcac ttagttcctg
    15301 taattcacca gatttttccg aacgaatcgc gtcaaagttc tttactacac tcgaataagt
    15361 cgttgttaaa taaagattca ttctagcata cattgctata tcatctttaa attctttatc
    15421 tgatggcaat tctacgaata ataagttacg ggaactctta agcgcaagtt ctgcctcttg
    15481 acctgctaaa tcagagtatg gtgaaataaa attaactgtc atttcagcat ttccagcttg
    15541 cttgtatgga cgttgatcta acacttgtgc ataagtaaac gtataccggt ttttaaattt
    15601 atttccacct gacatgccta atttttcata ggcaaaacta tttcggtcta gaacgccgtc
    15661 aaaaattcgt ttagatagtt ctacgatcaa atcactatct gtaatatttt gatttttaat
    15721 ctcacgagta atttcctgtt cttcatcagt ttggaaaata taactatcta agttcttccg
    15781 cacaaagttt tccttaatta aaaggtccaa tgcttcttct actcgttttt tagtttccag
    15841 gcggtcttcg ttgatatttc ttatcatcaa gctcgtaata ttttcaacat ttggtttgat
    15901 ggcttgatca acatatttaa tcatgaataa aattcgtaag acatttacgt taaagttatc
    15961 tatatcagtt ggtctttgaa tatgagaatt atttgtagct tgtgtaatca ctcggctaat
    16021 tgctgtatca atccaacgtt ctaacgagta ataaaactga tctaaagaaa ccaaactggt
    16081 tgtatcattt tccttatttc gttcagcggc ttctttaaac attgctagta atgaacgttc
    16141 accagacgat aagtttgatc cttgatagct gtgattacga atctcggtta gaatttgccc
    16201 caataaattg aattgatagg gaataaatgg ataaacttct gcgtaatttt cagcatcttg
    16261 gaagagcttt aattcaggac tatcaacaaa gagtaattta tttttcaatt ctgtttgaac
    16321 agtttcaaat tgtaaggcta aagaatcatt ggcaaactct tttttcttca aaatacgttc
    16381 tttaattact tcatcggcat ttgctgaaga taaactcaca gttgtggcaa aacgatcctt
    16441 gatcttagaa aaatcattat cagaattgtt atcaaagttt gttacgtgat caatcgcttc
    16501 ctgacttgtc actaataccc aagccttacc ttcacaaatt cgacccaaat cttccacaac
    16561 ggtttgtaac tgcaaagtca actcacgatt accgccgata tattgtccga cctcatcgac
    16621 tagaaaggca atatgatggt ctgcttcttt ggaatcaaga tagcttttaa ctagatttgc
    16681 aaagtcatca atgcttatta aataatttcc tgatgctttt tctaaaaaat tatcaatagc
    16741 ggcttcactc ataaaatcga ttttaagtgc cgcttctttg atatcatctt ggatccaatc
    16801 aaactcatgg cgctcttctt gccattttgc cccattaatc tcttcaaaag cagctttgaa
    16861 tgcatcaaac ttttcttctt taattaaccg acgttcaaaa tctgctagat gtggaatcgt
    16921 gcttaatcct tgcatttcgt taaatacctt taaaaaaaca ttcacgattc cgtttttgtc
    16981 ttttttacca cctgcatcac tttttgaatc aatattaaac agaataacat ctgtatttat
    17041 ttgtgagcac cgttccatcg ttgccaaagt cagtggatct ttgattttcc catcttcttt
    17101 gaagtaatca atggctttct tgtcatttat ttctcgattt gcgagcaagt aagaaataat
    17161 ttttaagaaa tgagatttac ctgaaccaaa gaacccctga atccaaacac ccattttatc
    17221 tgttggtgta ttgatactcc gttggtaggc atcaaaaatt gttttaaagt gtttttgtag
    17281 ttctttcgta accacgtatt cttctaattc ttgttgaaca ttttcatcct ttgcttgacc
    17341 gaccttaata actccttgaa tatttcggtc aattggtttt gcaaaaatat ctttaattag
    17401 catttaatct cctaccttcc atcccaacta aattctaaat gctcgataat aattatcatc
    17461 tttattggta ttaagaattg tcaaacttaa ttcatcatat tcccctggat acatcatcac
    17521 aactggtacg cgatcaaata cctgatgcat tttatttaaa atagagtgcg ctctaattaa
    17581 tggatataat tcgccaattc ctgtcaggaa cacaactgat ttttctggtg tatgggcatc
    17641 cacatattca aaaaatacat tttcttcctc aggtaaatcc attttcaata aacgaccaat
    17701 gctttccact aatttttgta aaccacgtcg ttcttccatc ttaatcgctt tttcagtgaa
    17761 accgcgtttt tctagatact ctaacataat cacatagaga ttataaactt tcacttcata
    17821 attatcacca acagatgttc cagatatatc attaatataa tctcttacaa ttaactcgtc
    17881 tttcggatcg tagtcaaaga tataatagcc tacttcatta cctaaaccac gattcaataa
    17941 aaaatcttct ttttgaatta cttcattcaa cttttccaaa tgctcttgaa tcgataatga
    18001 cattataaaa ttcctcccag tgctagtaca atatctttac gattcttgtc aactaaccat
    18061 tgaatcaatc gtttatctaa taatatcgga gtcattcgtc catattcttt ttcccctctg
    18121 actaatcctg tttcaattag attcgtttga taagcggcaa taagccgttt aatcgtataa
    18181 tctttccatt ttgaaatatc ttctgaactt ctccgcttct ctgtaaaaaa agaacggtaa
    18241 tcaactggtg ttaattgcaa atttccttta cgtagatttt cagcatatac ctcagtcata
    18301 aattcattaa aaagatcatc aatcataagg atggaaatca aattcactaa cttttgatta
    18361 tctatattta actgattgaa aagttgcaat ccagcttcat ccagttgact aactctcctt
    18421 ttcacgttct gatagatttc tttttgacgg ttactgctca ctgcaaaaaa aatattttca
    18481 tgttcatttt tctctttaat ttctatatct gtatatcctt ctagtcgtaa ttgaatatat
    18541 aacttgaact ctgaaaacca aaagcgttta gaaactagac cagcagaata ttttttattc
    18601 aatagattgc ctcctaatca tctataattc aataagtaaa caagtgtttt ttgctcatcg
    18661 ttagcattca ttttttagta tctactatac aatacatatt tattatag
//
LOCUS       DACCCR010000056        18406 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCCR010000056 DACCCR010000000
VERSION     DACCCR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.063"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            155..2341
                     /locus_tag="IVK93_001888"
     CDS             155..2341
                     /locus_tag="IVK93_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6387173.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYL
                     ALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHL
                     AEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGL
                     LFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQ
                     RKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIE
                     FLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWF
                     AIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSAS
                     LKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2338..4131
                     /locus_tag="IVK93_001889"
     CDS             2338..4131
                     /locus_tag="IVK93_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6387174.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            4331..4645
                     /locus_tag="IVK93_001890"
     CDS             4331..4645
                     /locus_tag="IVK93_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6387175.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            4666..5043
                     /locus_tag="IVK93_001891"
     CDS             4666..5043
                     /locus_tag="IVK93_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6387176.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            5053..5826
                     /locus_tag="IVK93_001892"
     CDS             5053..5826
                     /locus_tag="IVK93_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387177.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            5848..7251
                     /locus_tag="IVK93_001893"
     CDS             5848..7251
                     /locus_tag="IVK93_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6387178.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            7433..8617
                     /locus_tag="IVK93_001894"
     CDS             7433..8617
                     /locus_tag="IVK93_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6387179.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            8614..8904
                     /locus_tag="IVK93_001895"
     CDS             8614..8904
                     /locus_tag="IVK93_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387180.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            8901..9122
                     /locus_tag="IVK93_001896"
     CDS             8901..9122
                     /locus_tag="IVK93_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387181.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            9164..9943
                     /locus_tag="IVK93_001897"
     CDS             9164..9943
                     /locus_tag="IVK93_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP6387182.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            10010..10504
                     /locus_tag="IVK93_001898"
     CDS             10010..10504
                     /locus_tag="IVK93_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6387183.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            10560..11198
                     /locus_tag="IVK93_001899"
     CDS             10560..11198
                     /locus_tag="IVK93_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387184.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            11270..11665
                     /locus_tag="IVK93_001900"
     CDS             11270..11665
                     /locus_tag="IVK93_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6387185.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            11649..14102
                     /locus_tag="IVK93_001901"
     CDS             11649..14102
                     /locus_tag="IVK93_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6387186.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            14099..16126
                     /locus_tag="IVK93_001902"
     CDS             14099..16126
                     /locus_tag="IVK93_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387187.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            16123..17145
                     /locus_tag="IVK93_001903"
     CDS             16123..17145
                     /locus_tag="IVK93_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6387188.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            17162..18091
                     /locus_tag="IVK93_001904"
     CDS             17162..18091
                     /locus_tag="IVK93_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6387189.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            18336..>18406
                     /locus_tag="IVK93_001905"
     CDS             18336..>18406
                     /locus_tag="IVK93_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="regulates the transcription of tet(M) by
                     attenuation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP6387190.1"
                     /translation="MILKYPENICMLCIPMVMHKNPS"
BASE COUNT         6394 a         2955 c         3526 g         5531 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa
     1081 atttctttca atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct
     1141 aaatatgatt tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact
     1201 acttaatatt atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac
     1261 tctgttcgaa ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc
     1321 tgtacattta gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg
     1381 caaatctatc gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa
     1441 aaagaacaat ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg
     1501 attgcttttt tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc
     1561 tcaaatagta aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa
     1621 tgttggtagt acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag
     1681 aaagtgtgct attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta
     1741 taaaagcact gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa
     1801 tgaaaacaat tgggttaatt gtttttatgc taatacaaca tttgaaattg agtttttaga
     1861 tgtgaataaa gaaaatattc taacttatgt cagtccaatt atagatgacg cattcaaaga
     1921 taaaaaaaca ataactgctt ataaagaaga aatacaaagt gaacaatatt tagaaagaaa
     1981 tttagcgatg ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg
     2041 tgaaaaaatt gactttttaa tcaaaattcc taagtatatt cttaatgcta ttgcttttgc
     2101 atgtcaggaa atagtagatc tctcaattta taaaaaaatt ctactccata caatcgactt
     2161 gtacgaggaa actacagaat tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc
     2221 tgatttgaat atagcaataa tttctttatt agagtacttt ggtaaagatg aggatgttgt
     2281 gtatctaatc aatcagatag aaaattacac aaaaatactt tatgctgagg tttcaaaatg
     2341 acaaatatct tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa
     2401 agtgaagata acctttatgt aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca
     2461 aggaaaattg cttaccaagc aattaaacac gatggatcac taaaaagaat aattgctatc
     2521 acatacacta atagagctgc tgaagaaatt aaggaacgcc taagtttact tagtatagat
     2581 gatgatatta atatttgggt ggggacaata catcagtttt gtcttgaatt tattatttat
     2641 ccttttaaaa tgaatctctc aagagtatca aaaggattta caattattga tgattatact
     2701 caaagagctt acgtcagcca aattaataat atgctgaatt taaatctcaa aacgtatgaa
     2761 ctgaacaaaa ttgatttgtc actttctcat gattttaaaa ttaatgaagt tcagtatact
     2821 caagtttctg aaatgtatca cactatgttg gaaaaaaata aagaaattga ttttgatatg
     2881 attttactag tagcgtataa aattttaact gtaaacagta acgttgcacg taatattgct
     2941 agtaatataa gggcgattta tgtggatgaa tttcaagata ctagagaatt acaatacaat
     3001 gtaatagcta aattacttca aaacaaccct caaattcagt caatgtttgt tggagatatt
     3061 gaccaagcaa tttatggatc attagatggt atagctaaat cagttgaaga actagaaatg
     3121 ctaactagtc acacttttca atcaaagacg ctacatggct gttacaggtc taatcagaga
     3181 ttagtagact tctactctaa ttttcagagt tgtccttacg agattgaaag cagagggaat
     3241 aataaaaacg accgtggttt tataagttat gattgcaaag ttactaagga ggatttaccc
     3301 aacataataa aaaatataat aaaggaaaag attgaaagcg gcattcatga aaaagatatt
     3361 tgtgtacttg ctcctcaaca ctatcctctg ttttcactgg gtgagtattt aaagaaagaa
     3421 ttaccgtatt gcaattttga tgctataaat atatcaccaa ttaaggtaaa taatcatagt
     3481 ttatttttca agttagcaat tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga
     3541 aagctaattg caaacgatat tttaattata ttaaaaaatg aatttggaat agaagttaaa
     3601 gattatttta ttgacttaga tttattacag ctaataaaca gtgtgaaaag aatgtatagt
     3661 ggagacgaca acggtgttga tttatacaaa tttgttacca aacatatatt tgttaatctt
     3721 aatattgacg agaaaaatta cccgtctatt tttaagcact atctattttt tattaacgaa
     3781 gtagaagatc ggataaaaaa taattcatta tctagtagtg tatctagttt taagaagatg
     3841 tttaaatctc gagatggtat cacacttact acagcacata aagttaaagg aaatgagtat
     3901 gatactgtta tcgctattaa tcttctaaac gggaaaattc ctcattggaa tgaaattttt
     3961 aatgtttctg ataaaggaat tagttcagcg agaaaactac tatacgtaat ttgttcaaga
     4021 gcaaaaaata atttatttct tttctcagag gtgggttatt ttacgagtaa aggagacttg
     4081 ctttatccca ctcaattatt agataaaata gaatttgatt ataatattta aaatacctag
     4141 gtagtcgttg ttttaaatat agtaggaatc ggacgctcag tacggagcgt ctattttttt
     4201 acccaatttt gaaaggaagt gaacttatga aaataaaaaa tcaagaatca aaaggtcgct
     4261 ctcccccctt taaaaccatc acacaaataa gattcattca aacataaaaa cagaaaggat
     4321 aggtaaaaat atggaactta aatatgtcat tcccaatatg gaaaaaacat ttggacaatt
     4381 agaatatgct ggtgaaggga acattgaaca acgtcgaata aatggcagac caaccattct
     4441 ctctcgtagt tacaatttat actcaacgat tcaacgagca gatgatattg ttgtcattct
     4501 tccctccgaa gctggcgaga aacattttga atccgacgaa aaagtcaaac tcatcaaccc
     4561 acatattacc gcagaaggtt ataaaattgg caatcgagga tttaccaatt acattatgca
     4621 cgcagacgat atggtgaaag cataagaaag gaaaggtgaa aaaatatgag attagcagaa
     4681 ggtattgtga ttgaaaaaga ccagacattt ggcacattga aattttccgc cttgcgtcgt
     4741 gaggttcgta tccagaatga agatggtacg gtttccgaag aaattaaaga acgtacctat
     4801 gacttaaaat caaaaggaca aggacgcatg atacaagtca gtatccccgc ttctgttcct
     4861 ttgaaagaat ttgactacaa cgcagaagtg gaattaatca atcctgtggc agataccgta
     4921 gcgacggcta cttttcgagg ggcagacgtt gattggtacg tgaaagcaga cgatcttgta
     4981 ttaaaaggac agacaaaacc tcatcagaaa tataaaaaag aagcgtccga aagtgaaaaa
     5041 taggtgttgc ttatgacaaa cacaccattg attttgaaag aaattccgaa ggatgaagcc
     5101 atttctttta ttcggcaata ccattattca aagatactcc ctagattgtg taaatacttt
     5161 ctagggattt tttcagaaga aaaattatta ggcgttgtgg aattaggctg gggtacgcaa
     5221 cccctacaaa ccatacgcaa actctttccc gacagttccc ttcaaaccac agattattta
     5281 gaaattggaa agatgtgttt tcttccagaa atgaatcaaa ccaattattt tggcagtcaa
     5341 gctctttcag ctctaattaa gtggttgaaa gaacatacgg actgtcattt tttatataca
     5401 ctggcagatg gaattgaggg aaaatgtggc tatgtctatc aagcgtccaa cttcttttac
     5461 tgcgggtatt tcaagacaag tgtctatcgt gataagcagt catgggaaaa gatacaccct
     5521 cgaagtgctc gccttttatt agaggaaaat gctcgctttg aacaggtaga gaaaaagcat
     5581 tggctttccc aggcattttg cgaatacaaa gggattgaga aaatcaatgg acgcatgttt
     5641 cgctatctct atccgttgac aaaagaagcg aaaaaactgt taggacacac tctctatcga
     5701 cgtcattatt atccgaaaga aaaaaattta cgctttgaaa agcgaatcgc ttatcagaaa
     5761 tatgaagcca tttctcaacc aacttttgat aaacaggctc gtatctacaa tacacaatta
     5821 ttttgatagg tggaaaggag gatttttatg ccattgcttc atgacagagg agagcgaatc
     5881 aaagccaacg ataagcactt ggtctatcat tttgtactaa tttcattgct ggtagttttt
     5941 ctagtcgtcc tactcccttt tcattggaga gcgttacaat cggtgaactg gcaagcagtt
     6001 cagttagata catttttcta tcaacttcat acgccatact tttggagcag tattctcact
     6061 gctctttttg tatgcggact tatcagctgg ctttattatc actatcgtat agattctata
     6121 aaacagttag agcaccgtca aaagttagct cgtatgattt tagaaaatca ttggtatgaa
     6181 gccgaagaaa ctcaatcaga gggattcttt aaagatttac ccagcaagaa aaccaaacaa
     6241 cgaatccgcc attttcctaa aatatattat cgaatgaaag atggactaat ccatattcat
     6301 gtagaaatca ccttagggaa atatcaagat cagcttttgc acctagaaaa caaactagaa
     6361 acaggattgt attgtgaatt agtggaaaaa acgttgaaag attcttatgt agagtatgtc
     6421 ctactttatg atactattgg caatcgaatt ggcattgaag acgtggtagc tcaagatgga
     6481 cgtgtcaaat tgatggaatc ggtctattgg gagttcgatt ctctccctca catgttaatt
     6541 gctgggggca ctggtagtgg aaaatcctat tttattctca cattgattga atctctcctg
     6601 catacaaatg caaagcttta tattcttgac cctaaaaatg ctgatttggc agacttaggg
     6661 gcggtcatga ataatgtcta ttaccgtaaa gaagatatgt tggcatgtat caatcaattt
     6721 tacgaagata tgattgatcg cagtgaaacc atgaaacaac accctaacta taaaacagga
     6781 gaaaattatg cttatcttgg tttgtctgcc aattttctta tttttgatga atatgtggca
     6841 atgatggata tgctaggtcg tgaaagtacc accgttattc ataaactcaa acaaattgta
     6901 atgttaggac gacaagcagg cttcttttta attcttgcct gtcaacgtcc agacgcaaaa
     6961 tattttagtg atgggattcg ggacaacttt aacttccgtg tggctttggg tcgtatgtct
     7021 gaaatgggct ttggtatgat gttcggaagc gatacacaaa aacaattttt cttaaaaccc
     7081 atcaagggaa gaggttacgt ggatacagga aaaagtgtga ttagtgagtt ttatacaccc
     7141 cttgtaccaa aaaggtacga tttcttagga gaaataggaa aagtcattca aaaaaagcaa
     7201 tctgagcctg taccgcacga agtgaaaggt acaggttcag acttgctttg attggtgtgg
     7261 cgttagccac gccaatcctt tagccccctc tatctaacag gggggcacaa atacaactga
     7321 aaacaggcaa ttctcatcaa ccaatgttga tggtacaaag gattgcggaa aatatgaaag
     7381 gtgtaaactc aagggtcata gtttacatct tttttagatt ggaggtttgc gaatggaaaa
     7441 cgaaaaaatt tggattaagg aattgaaaga aaagcgactg gcttatggcg tttctcaaaa
     7501 taaactggcg gttgcttctc atattaccag accttaccta tcggatattg aaacaggcaa
     7561 ggcagtccct actcaacagg tgaaagaaga tttactgaac gctttggaac gcttcaatcc
     7621 cgataaccca ttggagatgt tattcgacta tgtacggatt cgattcccta ccaatgatgt
     7681 ggctcaaatt atcggtgagg ttttacgttt gaatatggat tatatgctac acgaagactt
     7741 tggctattat tcttatcccg aacactatcg tttcggtgat attgttgtcc tagtctctca
     7801 tgatgtaagt aaaggtgttt tattagaact gaaaggaaaa ggctgtcggc aatttgaaaa
     7861 ctttctattg gctcaacatc ggagctggta tgatttcttt caagattgca tggaacataa
     7921 aggtattttc aaacgcttgg atttagctat caacgataaa acagggattt tgaatattcc
     7981 agatttggca aagaaatgta aacaggaaga atgcatttcc gtctttcgaa gctttaaaaa
     8041 ttatcgcagt ggtgaattgg ttcaccgtga tgaaaaacca tacatgggaa acacacttta
     8101 tattggctca ttaaaaagtg aagtctattt ctgtatatat gagaaagatt atgagcaata
     8161 cgtgaaaaat gatgttccct tagaagacgc agaagtaaag aatcggtttg aaattcggtt
     8221 aaaaaatgac cgagccaccc aagccatgaa agatttattg gcacatcaac aggcggaaaa
     8281 aacagccttt gaaattatca atcgctatat ccgctttgca gataaagacg acactaaacg
     8341 tcggagtgat tggaaaacta atgaacgttg ggaatggttt atcggtaaaa atcgtggagc
     8401 attacgccta accacacagc cagagcctta ttcttttgaa cgaacattga actggttaca
     8461 tcatcaagtc gcccctacat taaaaatcgc aagcatttta gatgttctca acggtacaac
     8521 cattatctcc accatgattc aggaagccaa actgacagaa aaacatgaga aattaattga
     8581 acaacaacat ctagccatgg aggatttgat tacatgagcc ttttaacaaa agaattaaag
     8641 aagttagggt ttcagtgtgg cattgagttt caagcgtata tacaaaatac agggaaatat
     8701 acgtctctca ttattgaggg aaaacggcaa gctggtgata ccatctatac ctatgatttt
     8761 tacaaggtga gattttataa taattatacc aatcgagtaa ccgtctatgg agagcattta
     8821 accccttttc aattattaag gcgtgtaaaa agctatatct attatcgaga aaaatatctt
     8881 aaggaaagga ggacaatcac atgaattttg ggcagaattt gtacaactgg ttcttggata
     8941 atgcacagtc attggtattg ttagcgattg ttgtcattgg actgttttta ggttttaaac
     9001 gagaattttc aaaactcatt ggcttcttga ttattgctct aatcgccgtc ggacttgtgt
     9061 tcaatgcagc tggcgtgaaa gatgtgttat taaacttatt taaccgaatc attggtgctt
     9121 aacagatatt tttcatgata aaataggaag tatatatttt gttatgaaag gatttatgaa
     9181 tatgtttaca aatgatcaat ttaaatctct tttaattaaa acattagaaa gaaagagctc
     9241 ttcaaattat tatgaaggag gtaacgagat tgtttctaaa atgaaaatat cagaggtaac
     9301 tttagatgag actgacgatt ttacttatta taaaggatat aaaaagggct ggaatacgct
     9361 ttgcacatat cttaaaataa gagttccttt tgatgattta gacttttttg aaagtcataa
     9421 agatttgatt acccagacag caagctccat atatgataaa caaggtgata atgtattagt
     9481 ggatactatt ttagtaccac ttccagagaa ttacgaagta attaacttta gtcagttgaa
     9541 aattagtgat gtggtttcac aagctattga agacgctgag tcttttatga gtaatggtga
     9601 atatcaacgt gcctttgaca gagttcacac tgcttttcat ggatatttaa ttgaaatact
     9661 aaaaaaatat gaaattacag ttcctagaga cgaaaatcta tctaaattat atagtaggat
     9721 acaacaatta attgaaaaag aaattcaacc tactgaactt gctgatatag tgaagacaac
     9781 gataaggtca tcaaatggaa tgattagctc tcttaatgaa gcaagaaata ggcattcact
     9841 tgcacaccct aatacaaata ttattggaaa gagagaagca aaactaataa taggtatttc
     9901 aagtacagtt acagattata ttagcggata ccttgataag tagcgtcatt aactgattgc
     9961 tacttttttt atgactattt ttataaaaag gatgtgataa gaattggaaa tgaaagtcta
    10021 tgtggcaaat ttaggacggt ataatgaagg tgaattagtc ggagcatggt ttactccacc
    10081 aattgatgag gaagaaatgg cagaaagaat cggtttaaat gaggactacg aagaatatgc
    10141 aatacatgat tttgagcttc cttttgatgt ggacgaatac acgccgatta gtgaaatcaa
    10201 ccgtctatgt gaagccattc aagaaatcga aggtacacct atttataatg aactcaaaga
    10261 aattcaaggc atgtggttta gtagcttgga agaattactg gaaaacaaag aggatattca
    10321 ttgttatagt gattgtgatt cgatggaaga tgtcgcccgt tattacgtgg aagaaacagg
    10381 acagcttggt gaagtccctt ccaatttaca aaattatatt gactaccaat ctttgggacg
    10441 tgatatggaa atagagggta attacctagt tacctctcat ggggtatttg aatattgtca
    10501 gtaagtttaa caatccgtgt tataattaaa ttaataaata gcacggagag gtataaatta
    10561 tggtaaatgt atttgaaaat cttaaactat taaaactaga catgaaagat aaaggctggg
    10621 tgatagattc cttttacttt cgttacaaac agcaaaatta tattgtactt gttaagctat
    10681 ttgaaaaaga ggaaaaagtt ccagaatatg ctctgttaaa actagaattt ttaaaggaaa
    10741 acgatttttc agatatgcta gctgtctatg ctaattcggt aaaattatat acagacacga
    10801 aaacaataag agaatatttt ggtatcgaat acagtagtaa cttgggagat gttttatttc
    10861 aatttagcca aactcttgct cgatttattc cgacagaagt tagtgagaag aaaaatgaag
    10921 accaaaaaga agccatgtgt ttctcattaa gtcaaagtga ttcagaagat cccagaaaaa
    10981 aatattgttt ttcagttaga agaaatcctt taaagggaaa tggtgagctg ggaaaacgaa
    11041 gtccttttaa tgacaataaa acgagattgt atagacccag cttatatgaa cgattagggt
    11101 cagataccaa tttaagtttc agatattcaa tgaatccaga tgatgaagaa actgatgaag
    11161 gtattattgc aaaatggaca aaaaataaaa ttgaatagtt aaagcattga aagagcaatc
    11221 acctattttg attgttcttt ttctattttt acggaaagga cgtgaagaca tgaaaaaaat
    11281 aagaagttac accagtattt ggtcagtaga aaaagtgatt tatgcgatta atgatttaca
    11341 actccctttc ccaattacct ttacgcaaat gacatggttt atcgtttctt tgtttgtcgt
    11401 catactcttt ggcagtttac caccactttc tctgattgac ggagcactac tcaaatactt
    11461 agggattccc attgttctca ctgggttcat gtgccaaaag acctttgacg gaaagaaacc
    11521 ctatggattt ctaaagtcta tcattaccta tgctctacgc cctaaagtaa cctacgctgg
    11581 caaacaaata cgattaaaaa aagaaacaat gaatgaatcg attacgattg taaggagtga
    11641 acaactagat ggcatatccg attaaatata tggaagataa tctcgtattt aatcatgatg
    11701 gcgaagtatt cgcttattat gaattaattc cctataatta cagtttttta agttccgatg
    11761 aaaaaattca agttcatgat aatttctgtc aattgattgc ccaaaatcgt gatggcaaga
    11821 ttcatgctct gcaactagca accgaatcaa gtattcgagc cacacaggaa caatcgaaaa
    11881 aagaaatcac tggacgatta aaagagatag cttacgaaaa aatagatgaa cagaccgaag
    11941 cgttaatttc tatgattggt gacaatcaag tagactatcg cttttttatt ggttttaaac
    12001 tctacctcaa tgaacaggaa gtatccgtaa aaaatgttgg gaaagaaatt caaaaagcat
    12061 ttgcggaatt ctttcatggt gtgaatcaca aattaatggg cgattttgtt tctatgccta
    12121 tggcggaaat cgaacgcttt tctaagatgg aacagttgtt agaaagtaaa ttatccagac
    12181 gctttcagat tcgtaaattg gagaaagatg attttggtta tattattgaa catctttacg
    12241 ggcaaacagg tgtccctttt gatgactacg actaccattt accgaaacga tacgaagata
    12301 aaaaagtgtt ggtaaagaaa tacgatattc tcaaacccac tcgctgttta atcgaagaaa
    12361 atcaacgcta tttacggatt gaacaggaag ataccactac ttatgtggcg tattttacaa
    12421 ttaatagtat tgttggtgaa ttggatttcc ctaatagtga aattttttat taccagcaac
    12481 agcagtttac tttccccgtt gataccagta tgaatgttga aattgtcgcc aataaaaaag
    12541 ccttatctac cgttcgcaat aaaaagaaag aactgaaaga tttagatagc cacgcatggg
    12601 aaagcgacaa cgaaaccagt actaatgtgg tggacgcttt agaatctgtc gatgaattag
    12661 aaagtaattt agaccaaacc aaagaatcta tgtacaaact aagctatgtg gtacgggtat
    12721 cagcaaagaa tttggacgag ctgaaacaac gttgtgatga agtaaaagat ttttatgatg
    12781 atctgtctat caaattagta cgaccttttg gcgatatgct aggcttgcat aatgaattta
    12841 ttccctcaag caagcgatat atcaatgact atattcaata tgtcacgtcg gattttttgg
    12901 ctggtcttgg tttcggtgct acacaaatga ttggtgaaaa ttctggtatt tacgtcggtt
    12961 acaatctcga tacaggcaga aatatttatc tccaacctag ccttgcaagt caaggaatta
    13021 aaggctctgt aacaaatgca ttagcgtccg cttttattgg ttcattaggt ggcggaaaat
    13081 cattctctaa caatatgctg gtctattatt cagtactctt tggcggacag gcaaccattg
    13141 ttgacccaaa agctgaacgt ggtgaatgga aagaaacctt acctgatatt gcccatgaaa
    13201 tcaatattgt taatctcacg agtgagaacg acaataaagg gttacttgac ccttatgtga
    13261 ttttaaaact cccgaaagat tcagaaagcc ttgccattga tattctcacg ttcttaacag
    13321 gtatttccag ccgagatagc gagaagtttc ctgtcctgcg taaagctatt cggagtgtaa
    13381 ctcaaagcaa acaacgaggg ttgttattgg tgattgatga attgcgaaaa gaagatacgc
    13441 ccatcagcaa tagtattgct gaccatatcg aaagctttgt cgattatgat tttgcccatc
    13501 tgttattcag tgatggcagt gtcacccaat ccattagctt ggaaaaacaa ctcaatatta
    13561 ttcaagtggc agatttggtc ttacccgatt ccaatagctc ttttgaagaa tacactacca
    13621 tggaattatt aagtgtggca atgctgattg tcattagtac ctttgcttta gactttatcc
    13681 actctgaccg ttccattttt aaaattgttg accttgatga ggcttggagt tttttacaag
    13741 tggcacaagg aaaagctcta tctatgcgat tggttcgagc aggacgtgcc atgaatgcag
    13801 gggtctattt cgttactcaa aacgcggacg atttactaga tgaaaagatg aagaataata
    13861 ttggcttgaa atttgccttt cgttccactg acctagtgga aatcaaaaag accttagaat
    13921 tttttggtgt ggacaaggaa gatgaaaaca atcagaaacg cttacgtgaa ttagaaaatg
    13981 gacaatgttt agtttcagac atctatggtc gtgtaggggt tatgcagttt cacccgattt
    14041 ttgaagagtt gcttcatgcc tttgatacca gaccgcctgt gcgaaatgag gtggaagaat
    14101 gacacaaaaa caaaagaaat ttcttcacta tagttggata acgctccttt gtgtgggcgt
    14161 tattttactt ttattaggca cgctgggcaa tgcagtacaa gcgacaggct tagttgatga
    14221 aaccattgat acaagcaatg aatactccaa gtatggtttg aatcaatatc agcttgatta
    14281 ttatgtggac aatagctggg gctggcttcc atggaattgg agtgatggca taggacaatc
    14341 ggtgatgtat ggattgtatg ccattactaa ttttatttgg acaatttctc tttatctatc
    14401 gaacgccaca ggttatttgg tgaaagaagc ctatgcctta gatttcatta gtcaaacatc
    14461 ccaagcaatt ggcgaaaaca tgcagacgat tgcaggcatt acaccgaatg gcttttccag
    14521 ttcagggttt tatgtcggtt tcctccttct ttttattctc attgttggaa tctatgttgc
    14581 ctatacagga ttggttaagc gagaaacgac gaaagtgata cgagctgttt taaactttgt
    14641 cgtggtgttt attctatcgg cttcctttat tgcgtatgca cccgattata ttgcgaaaat
    14701 caatgatttt tccaaagatg tcagtaccgc cagtctgaat attgggacaa aaattgttct
    14761 tcctaacacg gatagtcaag ggaaagacag tgtggacatg atacgtgaca gcttattctc
    14821 tgttcaagta aagcaaccat ggctattgct tcaatatggc tccactgatg tagaagcgtt
    14881 aggggaagaa cgtgtggaaa gtttattgtc taccagtcca gatacaaaca atggtgaaga
    14941 tagagaaaat gtagtgattg aagaaattga agatcatgac aatacctatt taaccctacc
    15001 gaagacgatt tccagattag gaacagtctt ctttttgttt gtattcaata ttggtatctc
    15061 gatttttgtt ttccttctaa caggaatcat gattttttcg caagtgctat ttattatttt
    15121 cgctatgttt ttgccaatca gtttcctatt aagtatgttg ccgagttttg atggcatggg
    15181 caggcatgcc attaccaagt tatttaatgt gattatgaca agggctggta ttaccttgat
    15241 tattaccgtt gcctttagta tttccactat gctttattcg ctgtcagcaa gttctccctt
    15301 tttcatgatt atgtttttac aaattgtgac ctttgcaggg atttatttta aactgggcga
    15361 tttaatgagc ctattcactt tacaaagcaa cgactctcaa aacatgggca gacaagtgtt
    15421 tcgcagacct cgtcaaatga tgaatcgaca aaccagaaga tttcaacgca gtatgagtaa
    15481 gatttttcgt ggaaaagcag ggtcaacgaa gcaaaagaca atgacaacaa agcagaatca
    15541 aaccaataaa gctaaccata ctcgtcaaaa tgaacggact acaccaaaga aagaaacagc
    15601 actttccaat gtgaaaaagc aacaaggcgg tatcgggcaa cgattaggtg aaaagacagc
    15661 ccatgtattg gacacgaaaa atcgtctagt caataaaaga aagcaggcaa aacaacagat
    15721 acaggcgaca ccaacccatg tcaaatatgc ccttcatcaa ggaaaagaga atgtcactca
    15781 taatgtaaca gactttaaac aatctatggc agataccaaa gctcaacgca aacaacagcg
    15841 tatgaaccag caggagcaaa aacgaaagaa cattgctcaa aagcgtttgg agctggataa
    15901 agcaaaagaa aaaagacgaa aagaaaaccc atcatctcct gttcagaata accagcagca
    15961 tgttcgcaag cgtcagcaaa ccgtcacgcc tgttttgtca caaaacacta aacgtgaaaa
    16021 tcatcagcga ccattgaaaa aagagcaaaa acaaaagaca ccaccgaagc aattcaatca
    16081 gaggaagcta aataaaagaa cggataagcg aggacaacaa acatgaagct aaaaataaca
    16141 gtgatttgta gtgtggtctt ctctctcttc ttttttctct tattattcct agtcattttc
    16201 tttgcagatg atacggacag tggcgaaaac aacaaggatt cttctattcc acaaggcggt
    16261 gtgaccgttt caccagaagt gttggcacac cgtccgttga ttgaaaaata tggtaaggaa
    16321 tatgggattg aagactatgt ttcttatatc ttagccatta tgcaagtgga atcgggcggt
    16381 acagcagaag atgttatgca aagttccgaa tctttgggtt taccgcccaa cagtttgagt
    16441 accgaagagt ccatcaaaca aggggttaaa tatttcagtg aattattaac cagtgcagaa
    16501 caacaaggag tggatataga ttctgttatc caatcttata attatggcgg tggcttttta
    16561 aattatgtga ggagtcatgg gaaaaaatat acctatgaat tagccgaaca gttttctaaa
    16621 gagaagtcgg gtggtcaaaa agcagactat cccaatccta tagccatacc tgtgaatggt
    16681 ggttggcgat ataactatgg aaatcagttt tatgttcagt tggtttcaca atacctaacc
    16741 gacacttccc caacagaatt tgatgatgaa accgtgcaag tcattatgga cgaagccttg
    16801 aaatacgaag ggtttcccta tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt
    16861 agtggcttaa tacaatgggt gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa
    16921 gatcagtatg acgctacgca agaaatctct atggaagaag cccaagcagg cgaccttatc
    16981 ttctttcatt caacctataa tgcaggcaca tatgtgactc atgtggcgat atatttagag
    17041 ggaaatcgct tttatcatgc aggcgaccca atcggttatg gcgacttaag cagtcgttat
    17101 tggcaagacc atctgattgg ggctagacgt gttattcata actaagaaag gaatgaatca
    17161 aatgattata aaaatcgagc gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc
    17221 gagcaagcaa agaaaagttc cgatgattaa agttggcacg cataagaaat taacctttgt
    17281 tttatgggtt ttattgattg gtagtgtcgg atttggaatc tataaaaatt ttactgccat
    17341 tgacactcat actgtccgtg aaacagaaat cattaaacag cagattgtcg ataccaacca
    17401 agtagaaagt tttgtgaaat cctttgcgaa agactacttt tcgtggcaac aatcccaaga
    17461 agccattgat aaacgaaatg aaaaattgac ccattatttg acggaagaat tgcaggtatt
    17521 aaatgaagaa atgattcgta aggatattcc tactagttct agtgtgaacg atatacaggt
    17581 gtggcaggtt tctcaagtga atgagaacac ctttgaggta ctgttttctg ttgaacaagt
    17641 tattaccgaa gacaaagaca aggaaactat atcgtctagc tttcatgtgg ttgtccatat
    17701 agatgaatca gataatatgg ttattatcaa aaatccgaca atgagtaaga aacctcaaaa
    17761 atcggactac caacccaaac agctagaaag tgaccataca gtagatacag aaacgatgga
    17821 cgaaatcact agctttttag aaacgttctt ccagctctat cctaccgcca cagaaaaaga
    17881 attgacttac tatgtaagta atcatgtgtt acctatgatt aacaaggaat atgttttcga
    17941 ggaattggtg aatcctatct ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt
    18001 gaaatattta gatcaagaaa caaaggcaac ccaaatctcg caatttgagt tgatattaga
    18061 aaaacaagat aattggaaga ttgtaaaata acaaatattg gtacatgatt acagatactt
    18121 tgtaatcatg tactcttttt gataaaaaat tggagattcc tttacaaata tgctcttacg
    18181 tgctattatt taagtgacta tttaaaagga gttaataaat atgcggcaag gtattcttaa
    18241 ataaactgtc aatttgatag cgggaacaaa taattagatg tcctttttta ggagggctta
    18301 gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat ccagagaata
    18361 tctgtatgct ttgtatacct atggttatgc ataaaaatcc cagtga
//
LOCUS       DACCCR010000057        18054 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCCR010000057 DACCCR010000000
VERSION     DACCCR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.064"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            214..498
                     /locus_tag="IVK93_001906"
     CDS             214..498
                     /locus_tag="IVK93_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP6387191.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IVK93_001907"
     CDS             complement(779..1198)
                     /locus_tag="IVK93_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP6387192.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IVK93_001908"
     CDS             complement(1200..1784)
                     /locus_tag="IVK93_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP6387193.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IVK93_001909"
     CDS             complement(1831..3306)
                     /locus_tag="IVK93_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP6387194.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IVK93_001910"
     CDS             3487..3798
                     /locus_tag="IVK93_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387195.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IVK93_001911"
     CDS             complement(3913..6249)
                     /locus_tag="IVK93_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6387196.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IVK93_001912"
     CDS             6391..7059
                     /locus_tag="IVK93_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6387197.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IVK93_001913"
     CDS             7062..8105
                     /locus_tag="IVK93_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6387198.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IVK93_001914"
     CDS             complement(8342..9223)
                     /locus_tag="IVK93_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6387199.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IVK93_001915"
     CDS             9382..10110
                     /locus_tag="IVK93_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP6387200.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IVK93_001916"
     CDS             10266..10583
                     /locus_tag="IVK93_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6387201.1"
                     /translation="MHIQIPTVLLGGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IVK93_001917"
     CDS             10576..11184
                     /locus_tag="IVK93_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP6387202.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IVK93_001918"
     CDS             11200..12030
                     /locus_tag="IVK93_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP6387203.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12680
                     /locus_tag="IVK93_001919"
     CDS             12168..12680
                     /locus_tag="IVK93_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP6387204.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="IVK93_001920"
     CDS             12706..13578
                     /locus_tag="IVK93_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP6387205.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="IVK93_001921"
     CDS             13754..14116
                     /locus_tag="IVK93_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6387206.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="IVK93_001922"
     CDS             14125..14877
                     /locus_tag="IVK93_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387207.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVKPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="IVK93_001923"
     CDS             14891..16693
                     /locus_tag="IVK93_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387208.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="IVK93_001924"
     CDS             16970..18010
                     /locus_tag="IVK93_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6387209.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         5662 a         3139 c         3730 g         5523 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcatcg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttggtgg tactgtgtta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa ggttgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgactagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg taatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aagacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgcat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg catgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtt ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaaatc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagact tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcaaacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttctggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cctggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctag gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgata ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtatagga gtagccttag ttacgatcgt
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctga aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagacat ggaaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggtatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt ttta
//
LOCUS       DACCCR010000058        17206 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCCR010000058 DACCCR010000000
VERSION     DACCCR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.065"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(93..1112)
                     /locus_tag="IVK93_001925"
     CDS             complement(93..1112)
                     /locus_tag="IVK93_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6387210.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1134..2141)
                     /locus_tag="IVK93_001926"
     CDS             complement(1134..2141)
                     /locus_tag="IVK93_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6387211.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2134..2799)
                     /gene="pgmB"
                     /locus_tag="IVK93_001927"
     CDS             complement(2134..2799)
                     /gene="pgmB"
                     /locus_tag="IVK93_001927"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6387212.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2792..5086)
                     /locus_tag="IVK93_001928"
     CDS             complement(2792..5086)
                     /locus_tag="IVK93_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6387213.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5346..7511
                     /locus_tag="IVK93_001929"
     CDS             5346..7511
                     /locus_tag="IVK93_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6387214.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7815..8648
                     /locus_tag="IVK93_001930"
     CDS             7815..8648
                     /locus_tag="IVK93_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP6387215.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8750..10450)
                     /locus_tag="IVK93_001931"
     CDS             complement(8750..10450)
                     /locus_tag="IVK93_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387216.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10957..12117
                     /locus_tag="IVK93_001932"
     CDS             10957..12117
                     /locus_tag="IVK93_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6387217.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12133..12576
                     /locus_tag="IVK93_001933"
     CDS             12133..12576
                     /locus_tag="IVK93_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6387218.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13059..13589)
                     /locus_tag="IVK93_001934"
     CDS             complement(13059..13589)
                     /locus_tag="IVK93_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP6387219.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13694..14245
                     /locus_tag="IVK93_001935"
     CDS             13694..14245
                     /locus_tag="IVK93_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6387220.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14398..14871)
                     /locus_tag="IVK93_001936"
     CDS             complement(14398..14871)
                     /locus_tag="IVK93_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6387221.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15009..16481
                     /locus_tag="IVK93_001937"
     CDS             15009..16481
                     /locus_tag="IVK93_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6387222.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16478..17188
                     /locus_tag="IVK93_001938"
     CDS             16478..17188
                     /locus_tag="IVK93_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP6387223.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5352 a         3093 c         3533 g         5228 t
ORIGIN      
        1 tacagataaa caatgtgtta gaagtagtat cttcttttac ataaaaagct gaccatagga
       61 taaatggtca gcgtatttag tgagtcagct atttagtatg ggttttctaa aggaacgact
      121 gtctctcttt tgatcaagcg atgagggatg accaaacgta cgccactgct caattcttca
      181 tggatcaatt cgcgcagctt ttgcatagcg acacgtccta attcggagat atcgatatcg
      241 atacttgtaa ggtaagggtg tgttaatgtt gagaagatcg agttgttgaa gctaatgacg
      301 gagagtgtct ctggaatgac atagccatac atttgcgcca actgcatcgt gcgaagggcg
      361 aagatatcgt caatgaccac caatgctgtg gcttcgtttt ctttcaacgt tttctcaaag
      421 gacagataat cctccggatg ctctagtact acactggata acgcaggaag accagccatc
      481 atcattgctt tttgataacc gaaatatcgt tcgaaataca aactttcatg agtcgtgttt
      541 gtcacaaaca ggatattctt atgtccattc tggatcaaaa attctgttgc ttgtttacct
      601 agaagctggt tatcattatc tacataaacg atttcgttct catgatgata aggctgtccg
      661 atcaaagtga aagggatgtg gttttggtat aaataatcaa tgacagtatc ttgactatct
      721 gaatacacta gaataaaacc atctacttgt ttttgtaaat gcatacgctg gacgttctct
      781 aacagcaatt caaaattttt agcagtagcg attgctgtag tcatatcata attccgcgct
      841 tcttcgttga ttgcttccat gatctcaaga taaaatgggt taccgatccg ctcttttgaa
      901 tcaagagggg gtaagattac gccgatggca ctggaaatgc gttttcctag attgcgggca
      961 gtcatgttag gaacataacc tagttcatcc ataatttttc ggactttctt ctttgttgtc
     1021 tcggaaatac taggatgatc gttgatgaca cgagagacgg tcgaggtggc aacaccggct
     1081 tttttcgcca catcttttac ggtaatagcc atgataaaaa cacctttctc ttgttattgt
     1141 gcagagaacc gataaatcgt ctgactgatc cgttcttgac caggatggag gttgatcgat
     1201 ccaaatttgg ggaaatgaac gatatcaggg atttcttgcg gttcgattgc taatccgtag
     1261 tttgaatgca tctgttttcc attgacagag aaatcagctt cgaatccagt cgtagaaaat
     1321 aataccatac ttttattaga agttgctata gtcatttggc gaccagaatg cttctcgaat
     1381 aattgtaagg aagttttgga aagcctagga tgatgcaaaa gaaatacatc atctagtcca
     1441 tctggataac atgcaaggat ctcttgcagt gttggggatt ttctaaagtc gattggcagt
     1501 ttttcttttt ttgtccaatt tccagtaggc agtttttctt gatctaattc aagcacacca
     1561 tctaaaaaga ccgttaactg atgttcatag atgtctcttg ttccatttcc tgaaaggttg
     1621 aagtaggcat ggttggtcgg attgcagatg gtttcaacaa ggctactagc agaggttgtc
     1681 atctctaatt cgttttttgt taaacggtaa gtgatcgtag cagtgattgg tcctggatag
     1741 gtagaaaatt catcttttaa gtaaaagacc acacctattg atgatggagt tttgaaaact
     1801 tctacttccc aaaattggaa agaccagcct ttcgtgccac cgtggatatg gtgtgatccg
     1861 cagtttttct ccaatctatg ctcaccccaa gaagcatccc ggatccttcc ggctacaggt
     1921 ccgacagttg ctccaaaaaa gcttttgtct gttagaacat cttcgaaacg atcgtaagac
     1981 aataaaacat tttctgattt tccttctttg tctggtgcga agatttggtg caggcgggca
     2041 ccgtaattga gaagaacgac ttctaagtag tcgttcttca atgtgatttt ctttatttca
     2101 gtctctggaa ctggtacgac ggagatctct ggattatcca tggtcatgcc acttttcttt
     2161 caagtaatcg atcgtaagaa aagtcgtatc agggacaagc gggatctcct ctccaaggtc
     2221 ttcagcacgt cccacgccaa ttggttgagc gccacttgag aggatggctt ggataccggc
     2281 ttgcgcgtct tcgatcccga tacattcttc tgcttttagt ccgacagctt ttgctgcaag
     2341 gaggaagata tctggcgcag gttttccgtt tgttgcttct gccgggtcgg caatagcatc
     2401 aaagtaaggt gtaagaccca ttttttcaag taggaatggc ccgtttttac ttgcagaagc
     2461 aagagcaatc ttgatttttt cttcttttaa agaatccagt aaaggcacga ccccggggaa
     2521 gacatctttt ggttcgatcg tctggatcat ctctaaataa taatcgtttt ttcttttcgc
     2581 taattctgca aattcttcct ttgaaaaaga gtgttctttt ccgccataag ctaaaagcaa
     2641 agagagtgag tcttctcggc tgacgccttt tagctgttcg ttgaatgtgt ggtcgatcgt
     2701 aatgccgatt tcctgaccaa gctttcgcca agcacgataa tggaattcag cagtatcggt
     2761 aatgacaccg tctaaatcaa acaatacccc tttaaacatt tgcttcctcc tttcttagcg
     2821 ttatttctag acgatccgct aaagaataag actgttcata gacgtaaatt tctagaggag
     2881 aaccttctaa taactcgaaa gtaacttgtt catctgtgac tgagatgaac aataaacgtc
     2941 cgcgataatt gatatggaaa gaataagctt tccaagtttt tggcagaaat ggtgcgaatc
     3001 tcaactgatc atgggcagtt ttcatttgag caaatccttg gacaatcgac aaccagctac
     3061 cagtcatgga agtgatgtgc aagccgtctt ctgtgtcgtt attgtaatta tccaaatcca
     3121 agcgagctgt tcgctggtac atttctacag ctttgctttc cattcctaat tctgctgcta
     3181 aaatcgaatg gatacttggc gaaagggaag actcatggac agtcattggt tcatagaaat
     3241 caaagttttt ttgcttttct tcttttgtaa aagtatcatt gaagaaataa atcccctgta
     3301 gtacatctgc ctgcttgatg aagcaagatc taaggatttt gtcccaagac caatgctgat
     3361 tcagaggaag atcgcttgtg ggaatatcgg atacaggcat gagatcttta tctaagaatg
     3421 tgtcatgctg gacaaagatt cctaattctt cgtcaactgg gaagtacatg ttctctgcga
     3481 tatctttcca tttggctagt tcttcttcgc tgatagtgat agcagcttct ttttgataag
     3541 ttaaataatt ttctcgcgtg taacgcaaga cccaagctgc gatcgtattt gtataccaat
     3601 tgttattgat attgttctca tattcgtttg ggccggtcac accgtggatc atgtatttat
     3661 ttgctcgttt tgaaaaatgg acacggtctg cccagaagcg tgcgatttct gctaaaactt
     3721 ccagtccttc atttttcaaa tattccgtat cgccggtgta attcgtataa ttgtagattg
     3781 cataggcaat cgcgccattt cggtgaatct cctcgaatgt gatttcccac tcgttatggc
     3841 attcgacacc agtgaaggtg accataggat aaagcgcgcc ttttaatcct tgttgctgag
     3901 cgttgtggat tgcttgcggc agttgattat gccgatattt cagcagattc ttagttactt
     3961 caggtttggc taaagcaaga taaagaggga cagcatacgc ttctgtatcc cagtaagtgg
     4021 ctccgccgta tttttctcct gtgaatcctt tggggccgat attcaatcgt tcatcttcgc
     4081 cgtagtaagt agagaatagt tggaacaaat tgaatcgaat accttgttgt gcttcttcgt
     4141 ctccttcgat gaccacatcg gcaagcttcc atcgttctgc ccaagccgct gtttgctctt
     4201 cttttgcttg gatataattg tttgtctggt tcgttaattc tgagacagct ttttctgctt
     4261 gctgcgcttc tggaatatct ctactagtaa gaacgataat ttctttttcc actgaccatg
     4321 tctcaccttg agataattct gcagacaagc aaccatgtaa tgctaaagta tcttcgtgat
     4381 agtcagggat gactgatttt ccattaacga aatggcgcat tgctgcagta acagtaaatt
     4441 gttcaatccc aaagttgttt ggaatagttt tagtcgtcaa atagccgatt tcttctatag
     4501 ttccacgatc aatctcattc caaaaatgtt catcatagtt gctgtcttcg ttttgaacat
     4561 ttccatccaa ttttgatacg agtttgactg ttccgctacc ttcaagcatt tctatagtta
     4621 aatagatata agctgattct tttttgacaa tgctcagaaa acgttcgaag gaaatgcgta
     4681 ctttattagt agaagtagtg attgtgaatg tacgagataa tacaccattt ttcatatcta
     4741 attcccagta aaagtcttct ggttccaaag aagccaagtc gatcagctga tcgttgatcg
     4801 aaatatctat cgcgataaag ttcaaggcgt tgatcacttt tccaaagtat tctggataac
     4861 catttttcca ccagccgaca cgtgtcttat cgggatacca gacacccgcc aaataagtac
     4921 cttgatggtg gtcccctgag taagtttctt caaagttccc gcgcatcccc atataaccgt
     4981 ttccaatact tgttaaagat tcttgcagtc gaagattttt tttatctaat tgtgtcgtgc
     5041 tgattttcca tggatcaata gcaaatagtc gtttgatctg tttcattttt ctcctcctaa
     5101 tgagctgttt tatacgttca taataaacgc aaccgattgc acaagtcaat agacaaaaga
     5161 aaatatttca tagaaaaaag cttaaaaaaa taaattttta ggagaaaatt atagaaggga
     5221 acacacgtat tataggtttt atagaatggt atagacagaa aaataaaaaa taaagcattt
     5281 acaaaaaaga aaacgcacgc tataatacgt ttaacgaaac cgattgcgta aaggagctaa
     5341 acggaatgaa aaagatattt agttttgaat tttggcaaaa atttggaaag gctttaatgg
     5401 ttgtggtagc agttatgccg gcagctgggt tgatgatcag tatagggaaa tcaattccct
     5461 tgatcaatcc tgattggacg ccgcttgtca caacgggcgg agtaattgaa aatattggtt
     5521 gggcaattat tggtaatctt catattttat ttgctttagc aataggagga agttgggcaa
     5581 aagaaagagc aggcggagca tttgcagctg ggatctcatt tattttaatc aatcggatca
     5641 ctggggctat ctttggtgtc acaagtgaca tgcttgcgaa tgaagatgca tttacccaca
     5701 ctctgttcgg tacgaagatc atggttaaag gattttttac aagtgtattg gaagcaccag
     5761 ctttgaatat gggagtattc gtagggatca tcgctggatt tgtcggggca atggcttata
     5821 ataaatacta taattataga aaattgccgg atgctttgtc atttttcaat ggaaagcgct
     5881 ttgttccgtt tgttgttatc ttatggtcaa caatcgtggc attgattctt gctgtggtgt
     5941 ggccaaacgt acaggctgga atcaataatt ttggtttatg gattgcagaa tcacaagaaa
     6001 gcgcaccgat tctagcaccg tttttatttg gaacattaga acgtttacta ttgccatttg
     6061 gattgcatca tatgctgacg attcctatca actatacaca gcttggcgga tcgtatgaga
     6121 ttttatcagg cgcacaggcg gggacacaag tatttgggca agatccgtta tggttggctt
     6181 gggccactga tttagtgaat cttaaaggtg caggggatat gtcgcaatat gagtttgtgt
     6241 tgcataactg gacgccggct cgtttcaaag taggacaaat gattggttct tcaggtatct
     6301 tgatgggaat ggcttttgcg atgtatcgta atgtcgatcc tgataagaaa gcaagatata
     6361 aatcaatgta tttttcagca gcgttggctg tctttctaac aggtgtaaca gaaccattgg
     6421 agttcatgtt tatgtttgct gctgtgccat tatatgtcat ttattcagtg atccaagggg
     6481 ctgcatttgc gatggcagat atcttgccat tgcgggtcca ttcatttggg aatatcgaat
     6541 tgctgactcg gacaccgtta gctatcaaag cgggtctagg tggagatctt atcaacttcg
     6601 tgttgtgtgt gatcgctttt ggtgttgtta cgtatttctt agcaaatttc ttgatcaaaa
     6661 aattcaactt tgctacaccg ggacgtaacg gaaattacga caatgacagt gaagaaaccg
     6721 ttgtatctaa cagcggaaca ggaacagcag atcagcaagt ggtccagatc attcatttgc
     6781 tgggaggaaa agaaaatatc aaagatgtcg atgcttgtat gactcggtta cgcgtaagtg
     6841 taaatgatcg agaaaaagta gggactgaag aagcctggaa acgagcaggg gcgatgggat
     6901 tgatcgtcaa agacaatgga gtccaagcag tttatggacc aaaagcagat gtcttgaaat
     6961 cagatatcga agatctgctg caatcaggag cagagattcc agagccagaa atggtttcta
     7021 caggaaatgg acaagcaggt agtaagcaat acttaggtat agaacaagaa ctagtttccg
     7081 cagcttctgg agaagtgatt cctttatctg aagtaaaaga tccggtattc tcacaaaaaa
     7141 tgatgggtga cggctttgct gttattccaa catcaagaga agttgttgcg ccgatcgctg
     7201 gaaaagtgac aagtattttc cctagcaaac atgcaattgg gctggaaacg aaagatggta
     7261 tcgaagtttt gatccatatg ggaatcgata cagtccaaat gaatcagcca gcttttgaga
     7321 ttgctgtcaa agaaggccaa gaagtaggaa caggaacgaa attagctgag atggatcttg
     7381 atgtgatcca aaacgaagaa aaagatccaa cgatcatgat cgtatttaca aacgataaag
     7441 tagaagaagt agtgatcaag caattaggta cagtgacagc tggtaaagtg atcggctcca
     7501 ttaaaatgta aggagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaacagccaa
     7561 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgagaa gcagcagttt
     7621 gaatactgtt tagtggagag gtcgtgaaaa agctgccctg caccaagtga taaaaacagc
     7681 caaacaaaaa atagctcctg gtccattgag cttattactg aggagttagc aggagaacac
     7741 tatttgctgg agaggttgtg acaaaaattt tgtcacaacc cttttgtttc gaaatgaaga
     7801 caaggagaaa accaatgaaa ttcatgacac tcaacacgca tagctggctt gagccagaac
     7861 cagagaaaaa attgcaagaa ttagcagata agatcctgtt agaagattat gaagtgattg
     7921 ctttacaaga aatcaaccaa ttactggaat ctgaagaagt agaacctgcg aagctaatga
     7981 agttctgtcc ggtaaagaat caagtaccta tccgtaaaga taattttgcc taccgtttag
     8041 ttcagctttt aaaggaacac ggaaaagagt atttttggag ttgggaaatg agccacatcg
     8101 gttatgataa atatgaagaa ggaaatgcac tgcttacgaa aaaaacattg gaaagtcagg
     8161 tccttaccgt ctcccaattt caaaagaaag aagattatca gacacgcaag atcttgattg
     8221 ggaaaacaaa gatagatgat caggacattt ttgtctcgag ctgccacttt tcttggtgga
     8281 cagataaaaa aagtggcttt tattttgagt ggaaaaactt ggaaaactac tttttggaaa
     8341 ctcgcgtacc actttttttc ctaggtgatt tcaataatcc agtagactct caaggttatt
     8401 atactgtaag agaaagttgc cttcttcttc aagattcgta tgttgtcgcg aatgaaaaag
     8461 gaaaagcagc tactgtggaa aagaaaatag atggttggga acaaaataca gaaaaattgc
     8521 gtatagattt tattttcgtc cctgaaggaa tgcaggttaa aaaatatcaa cggatttttg
     8581 atgggatcga tagtccaatc atcagtgatc actacggggt ggaaatcgaa ttagatgtga
     8641 acgaataaaa aatataacat ataacataaa aaaactgatt aaaataaaac tctccaaacg
     8701 attataccgt ttggagagtt ttttgatcgg atgtatttat cccttttgcc taagcacgta
     8761 aaagcttgta tctatgataa ataaataaac atagcaaggt aaaccagcca atcaaagtaa
     8821 gagaaagaat gaaatagaaa tagattggcg tttgataatg aagcgtgagc tcgttgattc
     8881 ctttttgctg cttcacgatt ggggtcccga tatctgttac ttcataatca gtcaatgttt
     8941 ttccattttg ctgtagcaca gtccgatcgt agacgataac agggatatga acagttttgt
     9001 ccccagtgtt tttccacttg attgtcaaaa gaccgcctgg atgtgttttt ataaaagttg
     9061 tttcgttttc tagtatttct tcgttatacc ggtcgtattt attgtctttt gtctccttat
     9121 agattggcag atagtcaggt gtggtttttt ggaaaacgaa caagctggta gacatatctt
     9181 tgctgaagaa tgttttcctt gcttcttccg gcgtatcgaa tagataagta tgtcgactga
     9241 caaatgtttc attttcccac ttttccagat gctggtataa gttctggatc gtctgtgctg
     9301 aaaaaataag aatgccaaat atacttaaaa gacggaacca cttttgttca gaccactggt
     9361 tcagtgaaag ggcaaggtaa agtagaagca atagcgtaaa tggcacgaaa aatcgaaatg
     9421 gaaactggat caaactaata aaaggcacat ttttttcgat caatgtcgtc catggaatca
     9481 agctagtaga aagaaaaaag aaaacaagac ttgtgtacaa cacgattttt tggaataagg
     9541 tgttcttttg ttgaaagaaa ataaaaagca aaaaacctga taatatcaat ataggaaagg
     9601 ccttagggta atggagccaa taactgccat ccagatctat cgccttttga ctcatttctc
     9661 tgttaacaaa gggaggaaga atcttattcc cactgtaaac aatagctaat cctgcccaaa
     9721 tatttgctgt cagaacaaaa taaagcaaga ccgacttaat cagttgaagc agtgcttttc
     9781 ctttttcagt ctggttaaaa aagtaataaa gataaaaagg gatatacatc aaagctaaca
     9841 tgatcgaaga cagtaaatgg atctgtgtca taagtcccac tgagacagcg actttgataa
     9901 cagggaactt tttatgaagg acgaaatcag caatagggat catacataaa ggataaaaac
     9961 aagtccccca gctagtgaat ccttggttga tagaccagta ttgaatagaa tacgtactca
    10021 tgaataaaac ggctactggt acacttaccc aatttcgaac attgacatat ctcagtaaaa
    10081 tcagcataga aaatcctgaa ataagataaa gaatgaaatt tgaaagaatc tgataatgaa
    10141 accagctacc tgcaagcaac acgatcaatc cttgtaaata agcgaaaaat gggccgtaaa
    10201 cggcattcac gatcctgcca ctttgctgga atccataaag ggaaggaaaa taatggaaat
    10261 ccccatattt aatttgttgc gcggcatcat agaaccgatt gtaatggaat ggggcatcag
    10321 cacccaaaat tagtttatgt gtgaataaat gaggcatgag aaatgccaaa gcaatgaaaa
    10381 aaattgctaa aaatgggcca tattttttca attgtcgaac tcctttgcaa actttagata
    10441 aattcatcat atatcctatg cgagaggtag gcaagaaatt agaaaattca tgaaaatatt
    10501 tatgaatttt acaagaaaaa cataagtgaa agttctaagg agagaggatg cgacataagt
    10561 tttgttgcat ctccttgttt tgaaacagaa tttttcttaa tactcgaaac aaagtagctt
    10621 tttcatgacc tctcggtaaa cggtgttcaa tcagctgctt ctcggtaata agctcactag
    10681 cctagaaaat atgagaagct attttctgac tagcgctctt attactcaaa gcataacgca
    10741 gttttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10801 acccaacaaa aaatatgagt agctattttt ttgggctgta cttattactt gatgcaggac
    10861 agctttttca caacctcatc ggctttcatt ttctattttt ttcctgaacc catataataa
    10921 aagaaagaac aaaaaaactt gcaagggagg acacatgtga agcagagtaa gcaaaaacag
    10981 tctcgtcgac ttttgtttcg tttgttttta ttaatatgta tcgtcgtagg gttactgatc
    11041 gctttatatc cattttatgt ggattcttta aatagtttta ttgatcaaaa acgtatcgaa
    11101 caagtacaaa aacggaccgc agctgaaatt gaagtgcagc gaaagaaaat ggaggagaaa
    11161 aaccaacgat taactgacca aggattcaat cctggtactg atcctttcaa tgaaaagaac
    11221 agaaatgaaa gtacaacatc cagtcaatta gaggaatggc tgatcggaag tatcaatata
    11281 cctaagatcc aattaaatat ttcactttat gatcggctca atggtaggat ccttgaaaat
    11341 ggagcaggcg tcttgcaagg aacctctttc ccgcttggtg gaaattcgac gcattcagtg
    11401 atctctgctc acagcggttt acctaatcga cggttattta ccgaattaga tcgtttagaa
    11461 aacggagaca cgtttatttt gactgttttg ggggagaaat tggcttatca agttaaaaat
    11521 atacaagtgg tactgccaga tgacacgtct gttctaacga tttcagaagg agaagactta
    11581 gttaccttgc ttacatgtac gccgtatatg atcaacacgc atcgtttgct agtgacagga
    11641 catcggattc cttataacga atcggtaaaa aaagaagaag gaaaaggaaa tcaggaaaga
    11701 accatgcggc aatggtttat tcttgcagga acgataatag ctgtagtcat actgttagtg
    11761 tttatcggac gactaatcta tcagtataga ttaagtaaaa aagtgcttga tttttcattt
    11821 attattagtg attcgtcagg gaaaccggta aatggtagtt cattcatttt gaaacataag
    11881 aaaaaaacac ttactagaaa tggtgtgccg ttttcagtac aaagcgacca tcatgggaaa
    11941 gtcaagctgg atcaattgcc tggtggaact tatcgaatcg tttcagctga tgatcctaaa
    12001 gttgctgttt cttttggtat tagaaaagta aaacaagaga agatatactt ttttgagggg
    12061 cgaaaacttg taaaagagct tcaaaaaaat ggtttttatt ttaaaatcaa tgaataagta
    12121 aggagagaaa aaatgatccg cacaacaaca aaattgtctg ccaaagaact ggaatctgtt
    12181 ctatcgattt ggctgcatgc taacttggat tctcatgaat tcattccatc aacttattgg
    12241 gagaatcaag tagaatctgt taggaaagct ttaccagatg cagtgattta cttagcagaa
    12301 atagatcaag aaattattgg ttttgctggg gtgattgatc agtttatcgc aggaatcttt
    12361 gtgaaaaggg aataccggca acaaagaatc ggtacgcaac taatgaaaaa aataaaagaa
    12421 accaataaaa cactgacttt gacagtctat caaaaaaatc aatcagctat tcatttctat
    12481 ctttcacaag gttttacaat tcaaacacag caaaaagatg aagcagtaaa tgaggaggaa
    12541 cttgtgatga aatggaatca aaacgctgat ttttgatgaa cagaataacg aaataatgct
    12601 cccagcctaa ttttttgctg ggagcattat ttcattttga agtcactaat ttaactgtgt
    12661 gaatatgcag ttgttacaat ctttgataaa cggtgttcaa tcagctgacc ctcggtatta
    12721 gctcaaaaaa cggcaaaata tgaggaacta tttttccatt tgtttctcta atactcaggt
    12781 caaaacgctg atttcacaac ctcttgataa acggtgttca aacggctgct tctcggtaat
    12841 aagcccacta gcctagaaaa tatgaaaagc tattttctaa ctagcgttct tattactcaa
    12901 agcataacgc tgatttcaca acctcttgat aaacggtgtt caatcagctg accctcggta
    12961 ttagctcaaa aaacggcaaa acatgaggaa ctatttttcc gtttctttct ctaatactca
    13021 ggtcagaacg ctgatttcac aacctctctt tcacaacctt attttactcg tttataattt
    13081 tcatcgaagt attttccgct acggatatca tctggcattc catcgtatgg ttcttcgcta
    13141 ataacatctt cattgtctat acctgcatca cccatataag tgatcgtagc aaattcagga
    13201 acaagtaatt ttggataagt ttcatatttt tcacgagctg cttccatttc tgcgatatgt
    13261 tcgttttgga aagtaacagt gatttctgga tgttcttcaa cccatttagg gaaaaagatc
    13321 attccgtgaa gtttcttttc atttggcata aagtccaatg ccacgtctgt ccctgttggc
    13381 tctgtccaag tgattttgaa tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct
    13441 tttacccaac ggcctttgac cattccgcca tggatacggt aatcaacagt atggtcattt
    13501 ttggcatacc attcatattc ccaaccattg tcatacgtat agataaagtg agttccaata
    13561 aaatcttcca attctttaaa ttctttcatg tttaagcatc tccttataaa ttaaaacatg
    13621 taataaacta catgttttcg attactcatt tatgatagaa tggatttaaa gatttgtcaa
    13681 ggagggaaaa ctgatggcaa gaaaaaatac acttcgatat attttgttag ggttattaag
    13741 taagaagaag atgacaggat atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt
    13801 ttggcaagca aaacatagtc agatttatcc tgaacttgca aaaatggaag agcaaggaat
    13861 cattcagcat caagtagaga tcacaggtga aaaattggaa aagaaagttt atgaattaac
    13921 agaagaaggg aaggaacaat tggcagagtg gattcataca cccaccaatg aactaccggt
    13981 taatcgagac gaatttgttc tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt
    14041 agtagaaatt attcagcagc agcgtgacct tcatgaagaa aaacgacttc atctattaga
    14101 gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat gaaaattacg gacactacct
    14161 gattttagcg catgcgatca atcgggaaac agaatatgca aattggctaa cagatgtatt
    14221 agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca gtgatggaga attcctttct
    14281 atatgaaaga aacgcagatc accgttcgga tattcttatg ttcaaacagg ccttttttca
    14341 agtatttatg tagcatctac ttattaaaaa gcttggtact taggagatta gtggcgttta
    14401 gagttctttt aacaactgtt cgtagtcagc tttttcatcg acaggaagga gcttgtaagt
    14461 taattcgtct gtctcgttgt catgaatgat gtacatctta gcattctctt ctttgaagtt
    14521 accgatttgt aaaatcgtat agcgttcgtc gatcaattcg atatctttga ttgcttttga
    14581 aaaaacttta cggacatggt gcattttctt ttggcggatt tctgcctttt ctgcagcttt
    14641 tgacaaagta aatccgtcat ctaaatattt tttgatcagt tctactttta tgactgtagg
    14701 taaacgatat tttctaggac aattcgcatc agcttcgatc gaacgaatga atcccttttg
    14761 ttcccaatac cttaattgac gtggagacac acctacgata tcacttaatt cactgatgcc
    14821 aacaaccaat tgatcatttt ccaacaaacg tttaagggaa gccccaacca tttttgtcac
    14881 tccttttgtc ctcttgcttc ccttaattat atccgtatta acttatgtgt caagtaggtt
    14941 gacaaatagc gaatttagga ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa
    15001 ggagaaatat gagtaacaac caacccgtag atatttacgg aaaaccatat aatcggacac
    15061 ttttagttgt tgtcttatta attggtacat tttgtaccgt tttgaatcag accttattga
    15121 cgacagcttt tcctacattg atgaaagctt tcgatatttc agcgtctgat gtgcaatggt
    15181 taacgactgg tttcttgcta gtcaacggga tcatgatccc aataacagca tggctgatca
    15241 ataaattcag ttcaagaaat ttatatcttt ctgccatgac gatctttttg atcggaacga
    15301 ttacttgctt tacagcacct aattttagta cattactaat cggtcgtttg atccaagctt
    15361 gcggtgtggg agtatcgatg ccattattgc aaaatatcat gctatctatt ttcccgccag
    15421 aaaaaagagg atcagcaatg gggatgtcag ggatcgttat tggacttgct cctgctttag
    15481 gccctacatt atctggttat atcattgata actaccattg gcgtgacttg tttggtatgg
    15541 tactgccaat cgttgttctt gtcttggttt tagcattctt cctaatgaaa agtgttattc
    15601 cattgtcaaa tcctagcatt gatattttat ccgctatttt atcaacgatt ggattcggta
    15661 gtttacttta cggattttct agtgtaggag atgacggctg gggaagcttg aaagtaattg
    15721 gtttcttagt tgttggtgtg attttcatcg ccttgttctc atggcgtcaa ttacatttgg
    15781 aacatccatt cttggaactt cgtgtattca aatcatctac attcacgatt gcagctgttt
    15841 tagctggtgt aacaaatatg gcgatggtag gggcagaaat ggtcgtacca ttgtatattc
    15901 aaaatattcg tggagaatca gcattccatt ctggcttgat gttgttgccg ggtgcattga
    15961 tcatgggggc tatgatgcct gtgacgggtg cgattttcga taaatacggc gcgaaacgtt
    16021 tagccatcac agggatgttc attttgacag cggcaactgc accatttgct ttcttgacaa
    16081 aaacgactcc agttttatat atcgtgatcc tttatgcgat ccgtatgttt gggatctcca
    16141 tggtcatgat gccggtaacg acttctggta tgaatgcatt gccaaataac ttgatcagtc
    16201 atgggacagc ggtaaataat acattccgtc aaatcgcaag ctcgattggt acagctattt
    16261 tgatcagtgt gttgacgaat gtgacaaaag caaatatccc aagcaaatct gtattggata
    16321 atacgccatt agcttataaa gatcaggcaa tcaatgccac actgtcaggt taccatgccg
    16381 cgtttatcgt agcgatcatc tttgggattc ttggctttat tattgccttt ttattgaaga
    16441 aaaaagaacc tgttctaatg aaagcggggg ataatgcatg attattggag ttttgattgt
    16501 cagcttgctt gcttttgctt taacgaacgt gttcgctaaa aaaacatggc aaaccttttt
    16561 atctttgatt ttcggactaa tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc
    16621 caatcacttt ggaatggaaa aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc
    16681 tgattcccaa ggagcagata tgcttttgta taaagcttta gggaatggaa aagaaaaagt
    16741 gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa gcaactggta cggataacga
    16801 aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg
    16861 ggaatacaaa aacgacatgt ataaattctg gttcaatatt gctgataaca atcacgaata
    16921 tgacagccga gtgaatacat tcaaaatccc agagacatgg gtagaattaa gtacggacca
    16981 agcagccaag ttagctgaat tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa
    17041 agcttatgtt caagacggta tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc
    17101 agagcaagaa caacgtacga aagccttagc tgctgaattc caacaacaag cattcgcaaa
    17161 attagtgaaa gaagcaaaag gcgaataata ttttgaacac catgta
//
LOCUS       DACCCR010000059        16629 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCCR010000059 DACCCR010000000
VERSION     DACCCR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.066"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..1497)
                     /locus_tag="IVK93_001939"
     CDS             complement(<1..1497)
                     /locus_tag="IVK93_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6387224.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAE"
     gene            complement(1494..2180)
                     /locus_tag="IVK93_001940"
     CDS             complement(1494..2180)
                     /locus_tag="IVK93_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6387225.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2373..3794)
                     /gene="gndA"
                     /locus_tag="IVK93_001941"
     CDS             complement(2373..3794)
                     /gene="gndA"
                     /locus_tag="IVK93_001941"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP6387226.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(3964..4143)
                     /gene="rpmF"
                     /locus_tag="IVK93_001942"
     CDS             complement(3964..4143)
                     /gene="rpmF"
                     /locus_tag="IVK93_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP6387227.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4201..4770)
                     /locus_tag="IVK93_001943"
     CDS             complement(4201..4770)
                     /locus_tag="IVK93_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP6387228.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            4956..5858
                     /locus_tag="IVK93_001944"
     CDS             4956..5858
                     /locus_tag="IVK93_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6387229.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(5893..7677)
                     /gene="pyk"
                     /locus_tag="IVK93_001945"
     CDS             complement(5893..7677)
                     /gene="pyk"
                     /locus_tag="IVK93_001945"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP6387230.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7751..8713)
                     /gene="pfkA"
                     /locus_tag="IVK93_001946"
     CDS             complement(7751..8713)
                     /gene="pfkA"
                     /locus_tag="IVK93_001946"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP6387231.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(8881..12195)
                     /gene="dnaE"
                     /locus_tag="IVK93_001947"
     CDS             complement(8881..12195)
                     /gene="dnaE"
                     /locus_tag="IVK93_001947"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP6387232.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12426..12611
                     /locus_tag="IVK93_001948"
     CDS             12426..12611
                     /locus_tag="IVK93_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP6387233.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12673..13869)
                     /locus_tag="IVK93_001949"
     CDS             complement(12673..13869)
                     /locus_tag="IVK93_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6387234.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14039..15475)
                     /locus_tag="IVK93_001950"
     CDS             complement(14039..15475)
                     /locus_tag="IVK93_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6387235.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLEMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            15768..16577
                     /locus_tag="IVK93_001951"
     CDS             15768..16577
                     /locus_tag="IVK93_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6387236.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4869 a         3507 c         2925 g         5328 t
ORIGIN      
        1 ttctgccttt gcgataggaa taaagattct aaaaattgtc ccttgcccta ctacactttc
       61 tgctaagata cgtcctttat agttttcaac cagttgttta gcgatagaca accccagacc
      121 atttccaccc ttcgttctag ctcttgcttt atccactcga taaaaacgat tgaagatctt
      181 attaaggtct tctttgggta ttccttcgcc aaagtcctga atcgcgatct caaattcatt
      241 cagagtggaa gaaatagaga tatggacttc ttttcgtgta gttgaatact ttactgcgtt
      301 atccaatatg ataatgatta attgttcaaa gtgattacga taaatttgca gggtcacttc
      361 ttttgtcaaa tcatcatcta aagtgatcac atagtctgga tataatatct tgaaattatt
      421 ataaacttga taggtgactt cttttccttt agttgtttca ttcgcataat gaacatctac
      481 ttgttctgct cttgataagt ctagcatctc ttgaactaag cttttcatac ggctgatttc
      541 ttgcatgctg gcgcttaatg attcttctaa tatctcagga tcatctttcc cccaacgatt
      601 tagcagacta agatgccctt caataattgc aacaggtgtc cttaactcat gagatacgtc
      661 ttccacgaat tgctcttgtt gttcaatata caaacgcata cggtcaagca tctcattgaa
      721 tatttcagct aaatctgcca attcatcatt tgtgtcgatt tcaggcatgt ggatatctga
      781 ttgcggatct ttacgtatag tatccattgt atctctgagt accttgagtg gttttaagaa
      841 ataagaagat aaaaagaaac ctaaaatact acttaagatc aaagaaacaa cttctaaaac
      901 aataagagtc aataatagct tatttcgaat atcatagaaa gaagataatt catagaagct
      961 ttgcgcatag ccgatctttt ctcttgtacc tttagaatat ataggctgga tagataaaaa
     1021 gccagttttt ccttctagtg taacgatcgt tggaacttta cgtgtagtct gaatcaggtt
     1081 tcgactgttt tcatgggttt taaaaatcaa gttctcgtcc aaatcataaa cataaagatc
     1141 cattgatggc tgccctagct cagctataaa ggaatcgatc ttcattaagt ttccttcgac
     1201 cagtgaagta tgatctacga cctcatctcc gtcatttgtc gctttcgtca gattacgata
     1261 cgtatttact aatgtcagtt cttcatccga gttagccaaa cgagaagcta cttctgaaat
     1321 cgtacgctct acattgtttc tttcttttgc cacaatcaga ttgatagatg atttataggt
     1381 aatgacagcg aaaattgtaa acacaacgaa tatgaagaaa gaactcgcaa aggcccactt
     1441 gattgtcaag gagggccctt tcagttctct tttttccgat tttttcggct ttatcacgag
     1501 cgcatcacat aacctgttcc gcgtacagtt tggatatagc tttcctcccc gggaacgtca
     1561 attttgttgc gaagataacg aatatatacg tccactacgt ttgtttcaac ttctgtttcg
     1621 tatccccaca ctttatttag taaaacgtca cgtgctaata ccacgttcac gttttccatt
     1681 aaagtaagca gcaactcgta ttctcttttt gttagttcaa ttacttcatt tccgcgacgt
     1741 acgacacggt tttctttttc gatagtcaaa tcgcgataag taatagtagt ttgtttagcc
     1801 acatttttat cgccttcaat atcgattcga cgcaacaatg cacgaagacg tgctaataat
     1861 tcttcgatag caaatggttt gacgatgtag tcatctgctc cgtggtcaag tccagataca
     1921 cgatcgatca cagaatctct tgcagtcatc ataatgattg gtgtattttt gacttggcgt
     1981 acacgtctgc agacttctaa accgttcaat tctgggagca tcagatccaa caatatggca
     2041 tcccactcat tatttaatgc cgcatctaaa ccagttcggc cgttgtagtg gacttctgcg
     2101 ttataccctt catgcttcaa ctctagctcg acaaatcgtg ccaagttctt ttcatcttca
     2161 atgattagaa tattgctcat ttattatttt atcctttcta ttctactggc tttttaaaaa
     2221 ctctatcatt ataccgataa tatgcttaaa aatctatagt cattttatca tggatagcaa
     2281 ttttaaaaaa gagctgaagt ctactcataa caatcgactt cagctctttc gtcctctttt
     2341 tattttctca tttcctaaca gccgaaagac tattattctt catggtacca tgagtaatgg
     2401 aagattcctt ctttgtcgac acgctcatag gtatgtgcgc caaagtaatc gcgttgggct
     2461 tggattatat ttgcaggcag acgttctgct ctgtatgaat cgaagtaagc gatggctgat
     2521 gagaaagtcg gtacaggtac gccagcttgg acagccaagg caacgacgtc tctgactgat
     2581 tgctgatatt ttttcgtgat ttcaacgaag taatcatcta gcaataaatt ttcgatctca
     2641 ggatttttgt cataggcatc cgtaattttt tgcaagaatc gagctcggat gatacatcca
     2701 gcgcgccaga tttttgcgat ttcaccaaat ggcaagtccc aatcaaattc ttttgaagcg
     2761 acacgtaatt gagcaaatcc ttgggcataa ctcatgattt tactgaaata caacgcttct
     2821 cggatttttt cgaccaattc ttttttgtct cccgtgaact caaaatcgtt cgtacgagac
     2881 aaaatcttgc ttgcttgtac gcgttcttct ttataagcag aaatatagcg ggcaaataca
     2941 gattcagtga tcaatggaag tggcacacct aagtcaagcg cgctttggct tgtccattta
     3001 cctgttcctt tattcccagc tgcatcaagg atcacatcta ctactggttt acctgttcct
     3061 tcatcgtctt tacgtgtcag aatgtcagca gtgatttcta tcaaatagct gtctaattcg
     3121 ccttgattcc attctttaaa gatatcagcc atttcttcta ctgaaaggtt taaaatattc
     3181 ttcattaagt cataagattc agcaatcagt tgcatatctc catactcaat accgttgtgt
     3241 accattttca cataatgtcc ggctccattt gggccaatat atgtgacaca tggtgctccg
     3301 tcttctgctt ttgcagaaat ttgctctaag attggtgcaa ctagcgcata ggcttctttt
     3361 tgtccaccag gcatgattga aggtcctttt aaagctcctt cttcaccacc ggaaacgcct
     3421 gtaccgataa agttgatgcc agagttggct aactcttcat tacgacgcat cgtatcttta
     3481 aagaatgtat ttcctccgtc aatcaaaata tcgcctttat caaggtgagg aagtaactct
     3541 tggattgttg catctgttgc cggtcctgct ttgaccatta gcaagatccg acgtggtttc
     3601 tcgatagatt ctacaaattc ttcgattgta tatgttgctt tgaaattttt atctgggtgt
     3661 tcttctacta cagcggttgt tttagcccct gtacgattga ataaagccac tgaatagccg
     3721 cggctttcta tgttcaaggc tagattttta cccataacgg ccataccgac aacgccgatt
     3781 tgttgttttg acatatgaac tcctcctaca tacgggattt ctccctaatc tcttacatta
     3841 tagcttaaca cggctacatt gcaaactaaa gaacaacata ttttttattt accgactaca
     3901 aaaaaataaa cctcaaggct ccatggttag ccttgaggtt ttacattttc taattaaaat
     3961 gtattaagct tcttttgaca ttacgtcctt accatcatag taaccacaat ttgcacatac
     4021 gtgatggctt tttttcattt caccacagtt tggacattcg tttaaccctt tgattgtcaa
     4081 tttgtagtgt gtacgacgtt ttgctttttt agcttttgat gttcttctag ctggtactgc
     4141 cattttgcta cacctccttg taaagtcttg tatctaaatt agacacaata tattccaatc
     4201 ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt tcagatagtt ttgcaagacg
     4261 aggatcaatt ttattctcgg cttcttccat ttttctgcgc tggtattctt cttcggaaac
     4321 aacttcccaa tcattgcctt ttggaagttc tgttgctgct tcctcttctt ttgtcaatac
     4381 ccgcattgga atcgccaata agatgttgtc ttcaatcgac tctgctaaat tcaataatgg
     4441 accttcaatt agcaaaatct catctgcaga catttgttca tctctacttt ggaattgttc
     4501 ttctgtcatg aatacttcgt ctacagaaaa atccatgggc agttcaacag gttctagtga
     4561 acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa tgaacgatat agcctttctt
     4621 atcaactgag accaaacctt cgacttccac tggcgcaaca tcaagtatta aattgtctcg
     4681 cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa gttaacggtg tctcctggta
     4741 ttttctcaat tccaataaag accatttcat ttttcatcac tcctaagcaa caaaagcaat
     4801 tatacagggc aaaaagcatg ttgtcaatga aattttcttg acaccttgca gttcataaaa
     4861 aaacagaaaa atctttatta gaaagcctct gtttgctatc atcctcagaa tcatttacaa
     4921 taatcattat tccattaaag gagcggatga tcttcatgaa cacgaaagat atcgaatact
     4981 atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc taattatttc cgtgtgagcc
     5041 aacctactat tacatatgca ttacaacgtc ttgaaaaaga gtttgacacc gtacttatta
     5101 caagagatcg atcgcatcac gaattgatcg tcacgccttc tggaaagcag ctgtttctcc
     5161 atgcacaaac cattctccaa gagatttcta ctgcaagagc tgaaatcact cagttgcaaa
     5221 agaaaaagct tcgttttggc atgcctccta ttatcggcaa tgaatacttt cctaaactgt
     5281 caagttactt tatcaagcat gaattgatga atcaaattga gattaccgat ggaggctctc
     5341 gggacttata cgggatgatc cgacaaggga ggatcgattt agctgtatta ggctcaacac
     5401 agccaatcgt ggatcaatat ttagaaacag agttactttt ggacaaacgt ttcatgatcg
     5461 tcgtgccacc tacacatcct ctttccaagc aggatactgt ctctttcaaa gatatagcag
     5521 atgaacagtt tgtactattg aacgaacatt atgtccaccc cgcagcattc actaagcttt
     5581 ctaaacaaac acatactcat ccaaaagttg tttatcaaag caatgacttg acgattttaa
     5641 agagtatggt ccgagaaaat gtgggtatcg gttttttggc agagattgct gtccatcctg
     5701 aagatcattt agtttgcctc cctctttcag atgaagttca gcctcgcttc ttgatttcat
     5761 tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga acaagtagtc caagttatcc
     5821 atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa gaagagctgt ttttattttt
     5881 tattaagcta aattagatag ctgttgtttc gccacggtaa acgatgccgc gacgtgaatc
     5941 aacggtaatc aattcacctt ctgtaattgt tgttgttgcg cctttagcac caacgattac
     6001 aggaatatct tgtgcaattc ctacaactgc agcgtgagaa gtcaatccgc cttcttcaac
     6061 gataacagca gctgcttttt cgattgcagg catatattct ttgtcagttg atggtacaac
     6121 tagaaccatt ccgtcttttg ctttttttac agcttcttca gcagatgaag caataactgc
     6181 atttgcaacg acagattttt gtccaacgcc ttgagcttct aataatttag aaccaatcat
     6241 ttgaattttc atcacgttag ttgttccgcg ttcaccaact ggtacaccag cagtgatcaa
     6301 gattaaatcg ccttctgatg cgaaacctaa ttcaacagct tttttagctg ctagttcaaa
     6361 catttcatca gttgttgatg gtttttcagc aactgttggg taaacgcccc agtttaacat
     6421 caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag atatctgcat ctggacgata
     6481 ttttgaaatc atttttgcag tgtatcctga ttcagttgca gcaactattg ttttcacgcc
     6541 tagatttttt gcagcacgag ctacagataa accgattgtt tcagttacat ctgttttatc
     6601 gaattcgttc aattggaatg tacctaattc tgataatgca ttttcagctt cttggtcgat
     6661 acgtgccatt gttgaaacag cttcaactgg gtagtccccg tttgcagatt caccagatag
     6721 cattgttgca tctgtgccat caaatacagc gttagcaacg tcagaagctt ccgcacgtgt
     6781 tggacgtggg ttttcttgca ttgattctaa catttgtgta gcagtgataa ctgggatacc
     6841 agcagcatta cattttttga taatgcgttt ttgaaccatt ggtactagtt cagctgggat
     6901 ttcaacaccc atatctccgc gggcaatcat aacaccgtca gaaactttga tgatttcgtc
     6961 aatgttgtcg ataccttctt gagattcgat tttagggaag atttgaacat gagtcatgtc
     7021 tttttcttct aagatttcgc ggatatctag aacgtcttgt gctttacgta caaaacttgc
     7081 agcgataaag tcgatatcgt tgtctaaacc aaaacgaata tcgtcagcat cttttggagt
     7141 aatacctggc aaactaatag aaacgcctgg tgcatttacc cctttacgtg atcctagcat
     7201 acctgcattt ttaacttcta cgactaattc acggtttgct tcgtcttttt caatgatttg
     7261 catatcgatc aaaccatcat caaataaaat gtgtccgcca acgtgaacat cgtcaaataa
     7321 tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca tgttcaggat ccatagagat
     7381 gcgtgtttga tctcctactt caaatttgat gtatcctgca cgaccaaaat cagcttctgt
     7441 tgtgccttga acagttgtac ggatttcagc tccttttgta tctaaaagaa taccgacatc
     7501 ttttcctgtc tttttaactg cttcacggac catagtcata cgtgataatt gttcttcatg
     7561 gtcaccatga gaaaaattaa agcggaagac atttgcccca gattcaatca attgagaaat
     7621 aatttctact gagttacttg ctggtcctaa tgtactaacg attttggttt ttttcattta
     7681 aaaaactctc ccattcaatt ttgtttattt aaagctgtac acagcttaaa tagcttggtt
     7741 tctttcactc ttaaaatgaa agtgaattat ttagatcata tagtgataag tctggtttat
     7801 gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac gatttcgttg tcatggatac
     7861 cgacacataa tccacctttt ccttcttgaa gcaattcgac agcgtaagca ccgaatttac
     7921 ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg gacaacgtgt cccaagatag
     7981 atacacgtgt atgatagtca ccaaattctg aaagtttctc agcgaattca tttccgccca
     8041 ttacaccttc agccaagatg atcaagcaat gtttcttccc gcgatcgcgg ccttcttgga
     8101 tttttttagc aactgaagcc atatcaaaat catgttctgg gatgatgatt tcatccgctc
     8161 cgccagccac acctgaccat aaagcgatgt ctcctgcatt gcgtcccatt acttcgataa
     8221 cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg gtcaattgat tctagtactg
     8281 tattgattgc tgtatcaaaa ccgattgtga aatcagtccc aggaatatca ttatcgattg
     8341 ttccaggaat acctactgct gggaaaccgc gttttgtcaa agccattgcg ccatgataag
     8401 agccgtctcc tccgataacc actagacctt cgattccgaa ttttttcaat tgttcgattc
     8461 cttttaattg accttcttca gttgcaaatt ctggataacg tgcagaatac aagaatgtac
     8521 caccacgttg gatcttgtca cctacatctg ccacatcaag acgacgaata tctccagcaa
     8581 ctaaaccggc aaaaccataa ttgatcccgt atacttccag tccttcgtaa atacctttac
     8641 gaacaacggc acgaacagct gcattcattc caggtgcgtc tccgccactg gtcaaaattc
     8701 cgatgcgttt cataataaac attcacctca ttaaaatact ttatcctaag tatgacttag
     8761 gtttacaacc atttttgcgt taaccacgac taacattcta acattatttt tctcacttgt
     8821 cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa gtagtatttt ttaccgtaag
     8881 ttatttgaaa accacattat cttcgccaag cagataaata agttgccgct ttatactctt
     8941 gctgtcatcc acccaataag catctgacag aactactttt cgttgcgttt tttcgtgata
     9001 cataactact ggtatatatc ctgggtgttt ctgaaaaatc acttgtaatt gtttcattat
     9061 ctcgggatct tcccgttctt ctgtaatacg caaatagcaa gtttgtgaag gatactgggc
     9121 ttctaactgg ttaggatcag caaagctttc tccaattagt tgcagttcct gattatattt
     9181 gctgatttcg ctccgccctt gaacaaagat cactcgatca acggcaaaca aattacgata
     9241 tttacgataa agagttggaa aaactgtgac agaaatttct ccactactgt cggttccttc
     9301 aacaaaggcc atcagttctc ctttttttgt ccgaatttct ctaacttcct tgatcatcac
     9361 taataaattg actggttgat tagggacgat atctgtaatc agctgaactt gtttagctaa
     9421 acgaagacga tcaaaccctt ctgcaggatg acctgacaaa taaataccta agtactcctc
     9481 ttccagcttc aagcgttctt ctagtgtata gtcagcgact tcttcttctt ttaatgccat
     9541 gattcccagc aagtcaagac ttccaccact gtaaacgata ttttggattt ttccttcgag
     9601 atcaagcatc aattgtttcc tgttaggaga taattcatca aaaactccta ctgctgtcag
     9661 tgattgcagt aactctaatt tcagccaacg cttatcgatc cgtatgagga attgatccac
     9721 tgactggaac gggccatttt gttttctttc gtttaatata tcttctacaa aatcacgccg
     9781 aatacctttt atcgctgtca gtccgaatct gatttcttcc ttattgacca actcaaaact
     9841 ggaattactt tgattaatgg acggcgagga aagtttgagt ttatttttac gtgcttctgc
     9901 aatatattct ttcaattttg atgtgttatg ccgaacagaa ttcatcaggc tggtaaagaa
     9961 agcacctgga tgatgggctt ttagatacgc catttggaat cccacgaatg aataagcaaa
    10021 tgcatgtgac cgattgaatc catagtttgc aaatttttcg atatattcat atacggtcgc
    10081 tgctgttttt tccgaatatc cctgttgtaa tgctcctgat acaaaatgcg acttttcttc
    10141 atctaatacg tcttttttct ttttactcac tgcccgtcgc agaatatctg actgccctaa
    10201 tgtaaatcca gccatagtcg aggcgatctg catgatttgt tcctgataga cgatcactcc
    10261 atatgtgttt ttcaaaatcg ggatcagtga atcatctggg tagcgaatgg cttcttttcc
    10321 tttcttcctg gcaatgaagg tatctatatt ttgcataggg cctggtcgat acaatgcatt
    10381 gactgcggca atatcttcga tgttttctgg atctaatctt cgtaaaacgt tccggatacc
    10441 agcagattca aattgaaata ctcctgaggt ttcgcctcgt tgaaacaaga gcagcgtttt
    10501 tgcatcattt agcgggatct cttttaatcg gatctcatac ccttctactc gcttaacagc
    10561 tttcaaagta aaatcaataa tcgataagtt gcgtagtcct aaaaagtcca ttttcaaaag
    10621 accgattgct tctacctcat tcatcgtata ctgggtcaga aaagcttcat cagaaccgcg
    10681 ttggagcgga actagttcaa gtagattctt atcgctgata actacacctg ctgcgtgagt
    10741 cgaaacatgt cttggcaatc cttctaaaat cactgcagtt ttgaataaaa gttggttttt
    10801 ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt ttataagctt gggtcaatgt
    10861 gattttcaat tgattgggta ttgcttttga ccagcggttt gcttcacttt gcgataaacc
    10921 aaacactcta gctgcatctc taagaaccat ttttgctgcc attgtgccaa atgttgcgat
    10981 ttgtgccata tgatattgtc catacttttc tcgaacataa ttcaatactt cttcacgtcg
    11041 attgtcagga atatccaaat cgatatctgg catcgtaaag cgttcggggt ttaaaaagcg
    11101 ttcgaacagc aaatcatatt ctaatggatc cacatctgtg atttctaaca cataggctac
    11161 taatgaaccc gcagcagaac cacgacctgc accggtaacg attttgtttc tatgagcaaa
    11221 agccattaca tcccatacga tcaagaaata atcatcgaat cccatttgat ggatgacaga
    11281 taactcgtag tccaaacgtt cttggtattc ctttgtatag tcagtgattc ggcgttccac
    11341 ttgcgaatag cataactctc ttaaatattc tccagcttgt tttccattag gaactggata
    11401 atgcggaagt aatttctgat ggaactggaa ttcaaatgaa atcgattcag ctaaacgctg
    11461 ggcgcctatt acagcttggg aaagacccgc tttttcgaat cgtttgatca tttcttgttc
    11521 tgaaagaaga tagacatttg ctgttagtgg taaagatgca gataaatttt ctaaaggtat
    11581 ttggcttccc tctttgatat gtttcatcac ttcaagtgca aaaagttctt cttgttgtaa
    11641 actagcgatg ggatgtaatg cacaaagcga ataattttct tttatcacat actgatacca
    11701 ttcaggcaat tgatcaggcg tgttttctgt caagccgata taaaaatttc tagtaaaaac
    11761 attttcaagt aaaaactgcc aatgaccgaa aaagttatcc tctttattga aagcctcaga
    11821 aacagaatga tttgccggaa caacaaacgc tagatggtcg gtttctgtca tttctgcaag
    11881 ttccaatttt tcttggatca tttttcggct ggaaagtttc atcagctgct gataacctat
    11941 ttcatctttt gcgtacgcaa gcaagccata atttttttct tcataagtat aactaagatg
    12001 taatccaatg attggctgta ctccttcttc tacacaaata cgatagaatt caggtgcacc
    12061 gtataagaca tctttatccg ttatggctaa tgaagtgtag ccttgttttt ttgcttcttg
    12121 tacatattct cgaatacgta tcgtactttg taaaagagaa taagaagtaa ttgtataaag
    12181 ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt aaaattcact agcgttaaaa
    12241 ggtttgatcc ttttactttc attaagatag caggcgctga agttgattat tccacttcat
    12301 ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag aactcaaatt ttattgagat
    12361 ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa ttaagataga aaagaggtgt
    12421 tctttatgcg atatatcgtt acattattct gggctgtcgt tctaggtcaa gtagtcggtt
    12481 atattggtgc tgcattaaca agcggaacat atgattttac tttgacaaca atcatttcat
    12541 tcattgcggg tgtcattatc cttttgattg gtgcagttgc gccaagaaaa gaaacctctg
    12601 cccattcgta aaaaaacagc caagagcttg agatttctca agctcttttt tattttctac
    12661 tcctcaccac cattataatc tgctgcgaag aactttacga aagttgaata aagaaagaag
    12721 aatatttgac agaacaaaca aacttgtcac aataaaaaca gcactgtaat tataagcatg
    12781 cgcgactgtc gatcctagca tgggacctaa aacttgtcca aagttgctaa acatctgatt
    12841 gtagctatag attcggctga ctccttcttg aggcgtaatc ttactgatca gtgtattgac
    12901 agaaggcatc aaagcgccag ttgaaaagcc taaaataaag cgaagtatac ctaattggag
    12961 cggtgtagtg acgaatgcca taggtaaata acagcaaaat gataaaatga gtcctccaat
    13021 tagaactttt tgattcccaa tcttatcccc tagttttcct agtttcggtg aagaaaagac
    13081 agcggaaaca ccagctatcg aaacaatcaa gccgctgaca aaaaggatat tttctgtact
    13141 tccgctcaat tcacgaatat ataaggtcaa aatagggctg atcgtagtga ttcctatctg
    13201 taaaatcaag gtcgtaatga agagaccgat caaaatagag agatgatcca tttttgaaaa
    13261 aatttctttg gtgcttatca gatctttttt ctcgattgga tggaaatctt ctttgactaa
    13321 gaaaatggtc aataccgttg tgatcatcaa caaagcccct gtgatcaaga atacattttc
    13381 cataccaaac cactgagcca gtgcaccgcc cattgaaggt ccgatcagat ttccagcaat
    13441 cgctccggta gacaatgttc ctaacgccca tccgctcttt tctctaggtg cctgtgaagc
    13501 aatcatcgct gtggcatttg gaatatatcc agacaaaatc ccattccaaa accgcatgat
    13561 caaaagccaa tacgcgtttg gtacaaaagc caaagatccc attgttaatg tcatccctgc
    13621 tgctgcacgg atcatcatga gttttcggcc ttttcgatca gctagatttc cccagattgg
    13681 tgcaaccacg gcagatgcaa aagcagtcac agagattgct agtcccgaaa acaattcgat
    13741 ttgatctttt ggtgttccta gttgctcaac ataaactggt atgaatggca taactaaact
    13801 aatacttgca ccagtgaaaa aacagccaat ccacgcaatc actaaatttt ttctccaatt
    13861 aatctccatc tatcatccct ctttcgtctc ttttatcata cctattttcg aataaacaag
    13921 caacgatctc gccagtaaaa agagaaaaat cccgcaaagg tcattttata tgtaacgttc
    13981 tttttatgta aaacaaaaag agatcagaac aaatactgtt ccaatccctt ctctttagtt
    14041 aaattgttgc tagaatgatg aagttgataa tgaataaaat atcaacgatc cacaaagcaa
    14101 cagaaacttg attgtatttt cctttgatta ctttcacaat tgtatagaaa ataaatcctg
    14161 ctgcaatccc ataggaaatg ctatagcaca atcccataaa gatcgatgca aagaaggccg
    14221 gtaaagcttc ttccatatct agccatttga tgtctgcaaa agaagccatc atcattactc
    14281 caactaaaat caatgctggc gcagttgctt gatttggtac aatggcgatc agtggagaga
    14341 ataaactact taatgcgaat agcactgcca ctactactga agtcaaacca gtacgtccac
    14401 cggccccgat cccagctgca gattcgacat aagttgttgt attcgatgta ccaaagatag
    14461 cccccacaga agtagcgatc gcatcggcaa atagtgcttt atccattttt gttttgaacc
    14521 cacgaccatc ttccaacgcg atctcgtctt cttttgagaa aatacctgtg cggcgtcctg
    14581 taccgataaa tgttccgatc gtatcgaaag tatcagataa actgaatgcg atgatcgtca
    14641 tcaatacttg aggaatcttg gaagaatcac tgaataaaga ttgcatgcct tcagaaccaa
    14701 atgctgcacc gaaagttgtt cctaattctt taaatgaatt acctaacgaa ttcgtttgcc
    14761 aatcaatgga acttaaatca acaacaccca taaaaatacc aaggactgtg gtcgctacga
    14821 taccgataag aatagcccca cggacatttt ttacaactaa aatggctgtc agtaccaaac
    14881 caatcacagc caataagata ggagcgttgt caaagttggc taacgcaggg acgattccac
    14941 catttatcgt aacatttgtc gcttttccgc cttcaacgac agagctcgaa atagctgact
    15001 ggtctgctga aaaagataag aatcctgcat ttttgattcc gacatacgca acgaaaatac
    15061 cgatcccgcc accaatcgca tgttgaagac tttcgggaat cgcccgaata atcatttttc
    15121 gaatatttgt gacagtaata aaaatattga ttaatccgca aataaacacc attgccaagg
    15181 cttgttgcca agaataacca agcccaaaaa cgaccgtaaa agtgaaaaat gcgttcaatc
    15241 ccataccagg tgcctgagca tatggtacat ttgcgaacaa tcccatttcc agcgtaccaa
    15301 taatcgatgc aatgattgtc gctagaaata ctgcttggaa gggcatacct gatgctgaca
    15361 aaatcgtcgg atttacgaat aagatataac tcatggcaaa gaatgtcgtt actcccgcca
    15421 ttatttcagt tgatacattt gtattgtttt tctttaaacc gaaaaacttt tccataaaaa
    15481 gttccactcc ctgaattttt ttctgatgga aaactataat aataaaaaat aagtaaaata
    15541 gggcaaatcc atcgaacaat ttcaaattat atagacgttt cattcgcttt tcaacgtttt
    15601 tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt tagcttatgt ttagctattt
    15661 atatagccat aactgtagtt tttgttagga atagctgatt gctcttgtta attacctaaa
    15721 atatgctaga ctaaaacaaa tgctagttga ggaaggaagg acccttattg aataaaaaat
    15781 taattgccat cgatctggac ggaactaccc tcaatcaaga ttcgatgatt acatctaaaa
    15841 caaaagagac tctgaaaaaa gcaatcaatg ccggtcatca tgtcagtatt gctacaggac
    15901 gcccattccg aatgagccat cagttttacc aacaattaga attaacaaca ccgatggtta
    15961 attttaacgg cgcattagta cacatcccta atcagcactg ggatggtgaa agagaaacgt
    16021 tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa aaagcagcta aatttagatt
    16081 ttatcgctgc tgaaaatcgt gatacttttt ttattgacag ttttgatttc tttgatgaaa
    16141 aaatttttgc ttcttctcgt ccaggagaga agaatttgct ttctcctaaa aatttgacga
    16201 caaatcctac atctttgctt gtccgaacag acaaacgttt tgcaggagct gtatctgctg
    16261 aattaacacg tcaatttggc agttatgtcg atgttcggac atggggtgga cctactgcca
    16321 tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg cgtgcaagag attgctaact
    16381 acctgtctat cgatcaaaaa gacgtcattg cctttggtga cgagcataat gatttggaat
    16441 tgttggatta tgcaggttgg ggagtagcta tggctaacgg tactgaccaa ctcaaaggca
    16501 ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg tttagctgtt tatctggaaa
    16561 aactacttaa actataatag agaattagat tccacaaaaa agaggttgtg acaatttttg
    16621 tcacaacct
//
LOCUS       DACCCR010000060        16596 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCCR010000060 DACCCR010000000
VERSION     DACCCR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.067"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(55..789)
                     /locus_tag="IVK93_001952"
     CDS             complement(55..789)
                     /locus_tag="IVK93_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6387237.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IVK93_001953"
     CDS             1116..1526
                     /locus_tag="IVK93_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP6387238.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IVK93_001954"
     CDS             1519..2220
                     /locus_tag="IVK93_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP6387239.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IVK93_001955"
     CDS             2220..3806
                     /locus_tag="IVK93_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6387240.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IVK93_001956"
     CDS             3825..4046
                     /locus_tag="IVK93_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP6387241.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IVK93_001957"
     CDS             4043..4804
                     /locus_tag="IVK93_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP6387242.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IVK93_001958"
     CDS             5184..5693
                     /locus_tag="IVK93_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6387243.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IVK93_001959"
     CDS             complement(5768..6307)
                     /locus_tag="IVK93_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP6387244.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IVK93_001960"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IVK93_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP6387245.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IVK93_001961"
     CDS             7374..8171
                     /locus_tag="IVK93_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP6387246.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IVK93_001962"
     CDS             8199..8834
                     /locus_tag="IVK93_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP6387247.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IVK93_001963"
     CDS             8854..9627
                     /locus_tag="IVK93_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6387248.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IVK93_001964"
     CDS             10035..10832
                     /locus_tag="IVK93_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP6387249.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IVK93_001965"
     CDS             10810..11223
                     /locus_tag="IVK93_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387250.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IVK93_001966"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IVK93_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6387251.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IVK93_001967"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IVK93_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP6387252.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IVK93_001968"
     CDS             16000..16560
                     /locus_tag="IVK93_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP6387253.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5401 a         2962 c         3593 g         4640 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaat
//
LOCUS       DACCCR010000061        15980 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCCR010000061 DACCCR010000000
VERSION     DACCCR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.068"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(186..1553)
                     /gene="nhaC"
                     /locus_tag="IVK93_001969"
     CDS             complement(186..1553)
                     /gene="nhaC"
                     /locus_tag="IVK93_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP6387254.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1662..3083)
                     /locus_tag="IVK93_001970"
     CDS             complement(1662..3083)
                     /locus_tag="IVK93_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6387255.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3326..5203)
                     /gene="tdc"
                     /locus_tag="IVK93_001971"
     CDS             complement(3326..5203)
                     /gene="tdc"
                     /locus_tag="IVK93_001971"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6387256.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5494..6750)
                     /locus_tag="IVK93_001972"
     CDS             complement(5494..6750)
                     /locus_tag="IVK93_001972"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP6387257.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7330..8631
                     /locus_tag="IVK93_001973"
     CDS             7330..8631
                     /locus_tag="IVK93_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6387258.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8700..9299)
                     /locus_tag="IVK93_001974"
     CDS             complement(8700..9299)
                     /locus_tag="IVK93_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6387259.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9553..10434
                     /locus_tag="IVK93_001975"
     CDS             9553..10434
                     /locus_tag="IVK93_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387260.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10447..12057
                     /locus_tag="IVK93_001976"
     CDS             10447..12057
                     /locus_tag="IVK93_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP6387261.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12134..12793)
                     /locus_tag="IVK93_001977"
     CDS             complement(12134..12793)
                     /locus_tag="IVK93_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6387262.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13076..13741)
                     /locus_tag="IVK93_001978"
     CDS             complement(13076..13741)
                     /locus_tag="IVK93_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387263.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13888..14493)
                     /gene="dhaL"
                     /locus_tag="IVK93_001979"
     CDS             complement(13888..14493)
                     /gene="dhaL"
                     /locus_tag="IVK93_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP6387264.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14521..15504)
                     /gene="dhaK"
                     /locus_tag="IVK93_001980"
     CDS             complement(14521..15504)
                     /gene="dhaK"
                     /locus_tag="IVK93_001980"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP6387265.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15506..15901)
                     /gene="dhaM"
                     /locus_tag="IVK93_001981"
     CDS             complement(15506..15901)
                     /gene="dhaM"
                     /locus_tag="IVK93_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP6387266.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
BASE COUNT         4584 a         3338 c         2903 g         5155 t
ORIGIN      
        1 aagagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
       61 cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt aaaaagcaat
      121 tttcttgagt ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tcttttttcc
      181 ttccttcaat tttttacacg tcgacgattc aataaaaaac cgcctaatat tgtaaagaca
      241 ggtgcaagaa tcgagaaaaa ggcgaatggc aagtaatgca acgcgctaac tttcatcgtt
      301 cccataatga atgtgccgct gactccccac ggtacgagag aattgacggt cgcacctgaa
      361 tccgctagtg ttcgtgtcaa ataggttttt gataagccaa cgtcatcaaa agaacgcttg
      421 aatgtttctc ctggaagaat gatggacaga tactgttctc caacgatcag attgattccg
      481 atacagctca aggcagtaaa cccgactaaa cgagaaggtc ggtccatctt ttcttttaat
      541 tcttgaacga tggtatcaac gatcagatat ttgatcagta acccacctaa tgccaatgca
      601 agtaaaatca aactagcaga acctaacata ctcatgatcc cgcctcgaga aagcagttca
      661 tctagctttg gatcaggtgt atttgcgaca taaccattca tcaagatgtc tgctgctttt
      721 ccgaccgtca aacttttctc atggataaaa gaaagaacga gagcagctgt cgatcccgct
      781 agtaatgtag ggatagccgg aatttttttg attgcccccc caaataaaat aactagtgga
      841 atcaatgtcc aaggagagat ccaaaagctc gctttcatgt tttggatcat ttcattgata
      901 ctagctagat cggctgaatg actgttatgt cctaatatcg tataaaaaat caacgaaagg
      961 acgaatgctg ggaaatccgt ataacgcata ccagcgatat gttcgaatag attaactcct
     1021 ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg agcagttatt ccctaaaaag
     1081 gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga atccaagtaa atgaccgatc
     1141 cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg aactgccgac agtcactccg
     1201 acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac gcacagaaac aagtttaaat
     1261 ccataaacca taatcgtagg gatggttcca gataaaatcc atgaagcaac taagacacca
     1321 atcaaaagaa aaatcaggat tggaatgatc cctggagtga ttcctttgat gatcccgtca
     1381 tggatctcat cccatgtgaa ctttttaaat ctgccataaa acaacaaaca catgaaagct
     1441 gcaaggatcg ggatatgtgg cgagagctgg aatccgatga tcagtactcc taggagtgcc
     1501 aacaacacga tcaatacagc gatactttct tttaaagaaa tatttttatt catcgtcgat
     1561 ctcctatttc aaatgaaaag gccctgccat gaaaaaacta ttcacttggc aggacctcct
     1621 actaaatcag cgacaaaacg tcggctgaaa gacttatttt tttatttttc ttctaaactt
     1681 ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa tggaataacg
     1741 aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc tgtacctaat
     1801 gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac ccaagctaat
     1861 ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt cttacgcatt
     1921 ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt tgtagcattt
     1981 gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa agcaaataat
     2041 gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt gttttcttta
     2101 tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat tgtttttgat
     2161 ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat gaagctgaag
     2221 atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat aggctgcgtg
     2281 atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat cgcattgatg
     2341 acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata attttttgtt
     2401 gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg
     2461 ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc ttttgaccat
     2521 gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc caaaacacct
     2581 aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat gttcccgccg
     2641 acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat aactaaaata
     2701 caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt gtttcctaac
     2761 aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac ccataatagc
     2821 catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa ctgaggaccg
     2881 ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg accggcaaaa
     2941 aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc taacgtagga
     3001 atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc aagacccatt
     3061 ttacttactt cagatttact caccgttgac actctccttg attttttgga taaaataacg
     3121 gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt gaggggattc
     3181 tttgagttta ttgcttgaag ggtttcttca aagacaacct caaaacaaag ggatcgaaga
     3241 gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt agtattcaag
     3301 ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat tggtctgcgt
     3361 agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat tcttcaaagt
     3421 gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt acttttccgg
     3481 cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat gggctgttgc
     3541 catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg taaatgcttc
     3601 ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt gcaactaagt
     3661 catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga taagttaatg
     3721 gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag aagttgtaga
     3781 aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg acattcaaag
     3841 gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt gaaccttcca
     3901 agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa
     3961 agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg gcagaatatg
     4021 gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg attgctttat
     4081 acgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca ttgtatttgt
     4141 aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg
     4201 cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt
     4261 ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct tcttctgttg
     4321 atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga ccacgaacga
     4381 ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact ggaatcactt
     4441 ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt ttagtttgtg
     4501 gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag tgagctttga
     4561 tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct gttgacaagt
     4621 tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct ttcattgcaa
     4681 gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct aaagaaccat
     4741 cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat tccattccta
     4801 cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta ttaccattcc
     4861 atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag ttcatatgac
     4921 cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc gctgaaattt
     4981 ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg cttttttctt
     5041 ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct aaatgttcat
     5101 ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg tccccaatga
     5161 atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa ccctcctaaa
     5221 aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct gtaaactaag
     5281 ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt gttcacactt
     5341 ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc aaagattgta
     5401 ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt tgtaatagta
     5461 tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg ccaaaaagta
     5521 atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg atggatttat
     5581 gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt ttgaaacatc
     5641 ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt ccacgatatt
     5701 ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg cgatttcttc
     5761 cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt gcgcttcttt
     5821 caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc gttgtgcttc
     5881 acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt cttgagacaa
     5941 gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca accagaattg
     6001 gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt
     6061 tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga acgcttctgc
     6121 ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc cccattggtc
     6181 tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa agtcgataga
     6241 ttgcaggatt tggtaagtaa actctgtaaa tgagattcct acttccaaac ggctggcaac
     6301 gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc
     6361 aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat ttgcatcttt
     6421 accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca cttgttccat
     6481 cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc
     6541 gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca tcataaatgg
     6601 aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat aaagtgaaat
     6661 gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc
     6721 gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt ttttttatga
     6781 aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat aggaaataaa
     6841 aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc aatttgtatg
     6901 tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa
     6961 gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc accagctttc
     7021 tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt attaatatgt
     7081 gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt ttttaaattc
     7141 ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca attattgtta
     7201 aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact ttactttgcc
     7261 tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa aaagcaaggg
     7321 acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc tttaactatc
     7381 gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa agaaccaatc
     7441 attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa gcaacaacta
     7501 gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat tgcgttattg
     7561 gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac actaaaaaag
     7621 gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt ctttttccac
     7681 agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg atacaaagat
     7741 tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca aaatgatttt
     7801 gttttagtta atttgaatga tttgatccaa aaagacgaac aaggaaatct ttcctttaac
     7861 gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga tgtcagctat
     7921 tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa taacaatgaa
     7981 atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga catggttcct
     8041 ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc aaaaggtacc
     8101 ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc tcaatatgga
     8161 gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga acagttgaag
     8221 aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac tcaagcttca
     8281 ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc tatcctagga
     8341 aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca gccttattca
     8401 gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc
     8461 gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa tgctcggatc
     8521 aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat tcttgatcca
     8581 ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg attttacgtc
     8641 atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt ttttatgctt
     8701 tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc tgtatatgga
     8761 agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg aaaatagagg
     8821 atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc tcgatggaaa
     8881 agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc agaaaattct
     8941 tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt gttcactcga
     9001 ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa tggaagattt
     9061 tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa aaatacttca
     9121 taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact tacctgagcg
     9181 ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt catcaagtca
     9241 aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa accgttcatt
     9301 tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt tattctgagg
     9361 tttattataa ctatctattg agaaagtatc aagctagcaa ttccttgttt atgaattcat
     9421 tgacagaaat ttataaaaag aatattatgt actaaaataa taaaaaaagt tttataaaaa
     9481 ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt tagaaaaagg
     9541 agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt
     9601 ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt tggatttatc
     9661 ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat tataaaaaaa
     9721 gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt atctatccat
     9781 gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac tggaggagcg
     9841 atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg
     9901 attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc
     9961 caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt atttgatgag
    10021 cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt tgaaaaattg
    10081 aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt cgagcgacta
    10141 gcggataaag tcgtgatcat acgcgaagga acactcgatg aagaaggaac actcgatgaa
    10201 ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa tagtcactta
    10261 gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca atttgctgct
    10321 gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt gaaaaaattt
    10381 gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg atgaggagga
    10441 agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa ccttagccgt
    10501 gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg tggttttgtc
    10561 cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga aaccttgcaa
    10621 aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc tgagtatact
    10681 ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc
    10741 tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct gattgaactt
    10801 attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc tgaaagtatt
    10861 ctgatcaatt tgcttttagc aatatcgatc agctgtacga tgattttttt ccaagcggat
    10921 tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat ggggattttg
    10981 gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt
    11041 ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga tgtagctgat
    11101 tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc atttacaact
    11161 aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat aatcagttca
    11221 tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg tgaaggaaaa
    11281 ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct aaataaaggg
    11341 atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg ctctatctac
    11401 gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt tatccaatct
    11461 ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat ttgtctagct
    11521 gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca cggacgtatg
    11581 aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa tatgttacta
    11641 gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg gctttctgct
    11701 ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa agccgggttc
    11761 aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt cggatggctt
    11821 cctcgaatcg gtaaacttgc ttatatctac ttagcttact cctttgcact caattacttt
    11881 ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag ctgggttcca
    11941 cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg tatcagcctt
    12001 gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga aggataataa
    12061 aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca acatttggag
    12121 gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag acttctcgat
    12181 tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat aaagtttcgg
    12241 gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa aagtgatcct
    12301 tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct actttttcca
    12361 tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg attgctaaaa
    12421 gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt tgtctcaata
    12481 tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga acgcctagca
    12541 actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt tcctttccgc
    12601 tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat tgaggttctc
    12661 cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca agtgcgtgta
    12721 ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa acacataaat
    12781 gatcatgact cataatctct cctccttcta aacagctatt tctaataaag aaacataact
    12841 tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc gaaccagata
    12901 ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt ttgagacctt
    12961 aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca aaaacaaacc
    13021 ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt gttgtctatt
    13081 gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag gtttcagcgg
    13141 ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc atttgaaagg
    13201 ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac aaatagtcag
    13261 acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa taatccttga
    13321 tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac gctgcaatgc
    13381 taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg tattgttttt
    13441 gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt cggtacgcgc
    13501 tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta ggtttgctca
    13561 tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc catttttgcc
    13621 agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg ctctctttga
    13681 gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt ccaagttcca
    13741 ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat atggacaaat
    13801 agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg
    13861 atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta aaagcgttga
    13921 aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac gctctcctag
    13981 ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg cctggtcaat
    14041 gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc aaacatctaa
    14101 catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg ttccctgctc
    14161 gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg tcgctttcat
    14221 catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt tcgatatcat
    14281 cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg cttgaaaagc
    14341 ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat cgccaatcgg
    14401 ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa attgcgtgag
    14461 ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc ttgagatttt
    14521 ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg tctccgtcaa
    14581 gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat taccgacttt
    14641 tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat tcatgaacac
    14701 gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg cttcttttgg
    14761 taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta ctttagaagg
    14821 ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat actcgatctc
    14881 atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg ctcgaagcgc
    14941 tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca actcagctag
    15001 ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag tacctgcaac
    15061 tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat ctacgatcac
    15121 actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga agttcaatgc
    15181 atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatcgg cttccttaat
    15241 agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca ccgctgcact
    15301 taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc ctccaccaga
    15361 aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag attctacacg
    15421 atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga caatattgct
    15481 tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca ttttgtgctt
    15541 ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct gcaaaagctc
    15601 cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct tccaacatgt
    15661 cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca aaataactag
    15721 ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat ccgatctcgc
    15781 catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc tgctcaatca
    15841 tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata ctttttttca
    15901 caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc attaatgaac
    15961 aattcaagta tataataaat
//
LOCUS       DACCCR010000062        15960 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCCR010000062 DACCCR010000000
VERSION     DACCCR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.069"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..450)
                     /locus_tag="IVK93_001982"
     CDS             complement(<1..450)
                     /locus_tag="IVK93_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6387267.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IVK93_001983"
     CDS             674..1465
                     /locus_tag="IVK93_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6387268.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IVK93_001984"
     CDS             1431..2384
                     /locus_tag="IVK93_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP6387269.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IVK93_001985"
     CDS             complement(2782..3756)
                     /locus_tag="IVK93_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6387270.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IVK93_001986"
     CDS             3871..4305
                     /locus_tag="IVK93_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6387271.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IVK93_001987"
     CDS             complement(4430..5416)
                     /locus_tag="IVK93_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP6387272.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..6266
                     /gene="arsC"
                     /locus_tag="IVK93_001988"
     CDS             5844..6266
                     /gene="arsC"
                     /locus_tag="IVK93_001988"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP6387273.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6546..8468)
                     /locus_tag="IVK93_001989"
     CDS             complement(6546..8468)
                     /locus_tag="IVK93_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP6387274.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8555..10495)
                     /locus_tag="IVK93_001990"
     CDS             complement(8555..10495)
                     /locus_tag="IVK93_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6387275.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            complement(10783..11523)
                     /locus_tag="IVK93_001991"
     CDS             complement(10783..11523)
                     /locus_tag="IVK93_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP6387276.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            complement(11536..12627)
                     /locus_tag="IVK93_001992"
     CDS             complement(11536..12627)
                     /locus_tag="IVK93_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP6387277.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            complement(12617..13723)
                     /locus_tag="IVK93_001993"
     CDS             complement(12617..13723)
                     /locus_tag="IVK93_001993"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP6387278.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            complement(13742..14419)
                     /locus_tag="IVK93_001994"
     CDS             complement(13742..14419)
                     /locus_tag="IVK93_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP6387279.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            complement(14430..15209)
                     /locus_tag="IVK93_001995"
     CDS             complement(14430..15209)
                     /locus_tag="IVK93_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP6387280.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            complement(15238..15588)
                     /locus_tag="IVK93_001996"
     CDS             complement(15238..15588)
                     /locus_tag="IVK93_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP6387281.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            complement(15604..15960)
                     /locus_tag="IVK93_001997"
     CDS             complement(15604..15960)
                     /locus_tag="IVK93_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387282.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
BASE COUNT         4736 a         3344 c         2689 g         5191 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa atcatggcag
     6001 aagacggcat cgatatttcc aaacagacgt ctgatttgat tgatagagat tactttactc
     6061 aggcagactt gatcattaca ctttgtggtg atgccaaaga caagtgccct gccgttccaa
     6121 acaatcacga acaccttcac tgggacctaa aagatcctgc acaagctgtc gggagcgaag
     6181 aagaaatatt aacggaattc agaaaagtca gagaaggaat caaaagaaaa gttttagact
     6241 taaatcaacg tacatcgatc gtataaagaa gtattcaaaa tgatttttca agcactaaga
     6301 aacaatgtta aaaaaatagc tttccatatt ttacaaaatt gtgccttatt gccgacaaat
     6361 tgtctcttga acaccgttta ccagtagtca agacatcgta agcaactata aaaaattata
     6421 agaaactcaa aataaaagga tgtgccacaa ctttggccac atcctctttt tttgtattta
     6481 gttttctact tccaaataaa tagatagaat tattataaat ataaaggcag ttttttaatc
     6541 gctacttatt cataactaga taacaacgtt aagatttcta gcggatgatt ggattgcatc
     6601 aactgagcca tttgttctgg ctgcttttct agatagtgaa taacagaaaa tatttttttg
     6661 aactctgcat gtttcaaatc atttttcttt attgctaata gcagtacgat acgtatctgt
     6721 tccccgtgcc ataggactgg ttctttcatt gtagcaatag aaatcatcga atgcttcaca
     6781 tacagcggat taccgtgagg gattgttaca aatggtgcta ctctagtact ggagtgactg
     6841 tctctttcca agacagaatc gatgaattcc ggctccacat aatgatgttc cactaagacg
     6901 tttcccataa agtttaaaat atcggctggg tctgtccgtt caagttgagg aaagatcaga
     6961 aatggatgag taaagcttgc tagatcaaat gtttttttct tcttgatagg cacatgctcc
     7021 agcatatatt ttgtcaaata ggctagatca gtttctgtga tcattggtga gatctcgatt
     7081 actggaatgc ttatatgttt aaatggtaac gtagccaaga taaaatcaac agagggaaaa
     7141 tcttgtctct ttaattctgg cttatattca actagatcca taatcgttaa ttctggaagt
     7201 tctctgttca gccgtgcttc gagaaaagta gccatagctt ttgaatattc actaactaaa
     7261 accgtttgat atcttctact tttctgatgt ttgtgtcgct cgatcgctgc ttggaaatgg
     7321 atcgttaaat aagctgtctc ctcttgtgga acataaaaat tttccttttc aaaataatct
     7381 tctaaaatta actgtaaaac taagaaaagt tgtgtgtacg tactaaacac atttttcgtc
     7441 aatggattag aaatctgcaa accataatgg agtcgaaaat aagttgaaga taaatgagac
     7501 cttaaatttt gttttagtag ctgatcttct tttaatgaat agcctagaaa ctgttccact
     7561 tgttcgatca aatgatcagc aagtgcgcta gattcctttt gaaaacgtgt ggctgcttgt
     7621 gaatttgtaa aaagattggc gatacgcaaa gccagataat tgatttcatc aggagaaaat
     7681 tcaattggat agatggtggc tagttttgct aatatttgac tacttaactt ttgtgcttgg
     7741 gtgtgttcca cgacttcgtt ttcgtcttta cttaatttca ctttctggta ctgtttcatt
     7801 cgctcgagca taaagtagat gtgaatctcc acactagcca gtccattcgg ctgttctaaa
     7861 tggttttcat aaaaaacatc ttgtaatgta tgttggataa cttttaatgt atctttagcg
     7921 aagaattctt tcaacgacgg actttgtgga tcttctttct gcatatttcg taatgtctta
     7981 acaagcaact gtcttttttc ttgttcatcg cctttaagaa aagttcctag ttttgttttg
     8041 gatactagct gtaaatggtg atactcaaaa aagctttgca actctgtcaa ttctttacga
     8101 atcgtacgtt ttccaacata tagtttttct gaaagttctt caagactaaa tccgtctttt
     8161 gctactagca gatggaaaat caataactga cgaagctgtt ttttatccat ttctgtttga
     8221 gtctcttcat taagcgctcg tttcagttgt tccctttcgt gaggcagacc attcaacaag
     8281 atacctgtac ctttttttct ttcaaacatt aaaggcagat gtttgtctct caaccaagtt
     8341 tcaatggtat tcaaatcgct atgaatcgtt ctttctgaca catggaaatg ctctgctagc
     8401 ttttttgttg ttatataatt attgttctca cttaattgat agatcaaaga ttgctgacgc
     8461 tgattcataa tgtgcagaaa ctcccttcta aataaaaaat cgatcatgat aacacatcta
     8521 ttatgtctca tcacggtcga tttttcatct tatgttacat ttcgcttaat aattggtgaa
     8581 ctgttgtttt atctggcgct tccatcagtg cttgacggaa gtcatcatcc atcaatttac
     8641 gtgatagacg ttgcagtatt ttcaagtgca tatcaccttg gctgttttca ggtacagcaa
     8701 tcataaagat catatgtgca ggttttccat ctaaagaatg ccaatctacc ccagattgac
     8761 ttctggcaaa tgccactgtt ggtttaagaa cataagctga ttttccatgt ggaatagcaa
     8821 tccccatacc gattcccgtt gtactttcat gttctctatc atagatgact tctttaaatc
     8881 tggttttatc tgtgatcgcc gctgtttttt ctaaacgttg gactaaatct tcgattactc
     8941 cgtcttttgt tgttgctgtt tcacctaaaa cgattgcttc atctgacata atatccgtga
     9001 tagaatcaaa agtttcagtt ttcgtataaa catcacttgt ttcagtctct gatagatctg
     9061 ctactgtcac accatttgct gcagcaaccg cagtttgttg taatgtcggt ttaaggattc
     9121 ttaacattag cacggttaca gcaataccag caatcactgc tacaaaaaac atggctacgt
     9181 gatccactgc tcctaaaacc gctacgatcg gaccgccatg agctacatga tctgtcactc
     9241 ctgaaagcat cgcgatcaca cttcctgtca ttgccccgat tacaatgctt ggaatcaccc
     9301 gtagtggatc tgttgaagca aacggaatag cgccttctgt aatcccaaac agtcccattg
     9361 ctaaggacgc cttaccagct tctcgttctg ttttcgtaaa tttggttttt aaaatcaatg
     9421 atgccagccc caatgctaat ggagggatac agatagctac agcaattggc cccataatgg
     9481 aataattgcc ttctgcaatc aagccagatc cgaacaaaaa ggctacttta ttgaacggcc
     9541 cccccatatc aaaagcaatc atcgctccta agatcatagc tagaacaatt tcacttcctc
     9601 cttgcatccc tgctaaaaag tgagtcaatg aactaaatag aagagctact ggttctccaa
     9661 taacatagat aaacacaaaa ccaactgcca atgatcctaa aattgggata acaataattg
     9721 gcatgatcga ttgtaaagct ttcggtactc gcactttctt gattcctaaa gcaatgaatc
     9781 ctgctaaaaa tccagtgatg atcccgccta agaatcccgc attcgcatca ctaccatata
     9841 atgctccgtt tgccgctaac atcccaccaa caaatccagg aacaagtcct ggacgatctg
     9901 caatactttg cgcaataaat gcagatagaa ttggaaccat caaccccata gcgatacttc
     9961 cgatactatt catcgttgcc caaatcgttc cttctggtat cttgatccct tcaggtgtag
    10021 ctgttcctcc taatgttaaa gaaatcgcga tcaataaccc tcctgtaact acgaaaggta
    10081 ccatgtacga aacgccattc atcaaactct tatagatttt tttacctaaa ttttctgatt
    10141 cacttttatt ttcggaagaa ataattgcct ccgtactttt atagactggc gcttttcctg
    10201 ctaaaatatc tctcaccaac tcatctgcat ggtggattcc ttcttgcaca cctacggtta
    10261 tcaaaggttt accgccgaat cgtgatttat ctattttagt atctgctgcg atgattacac
    10321 cagctgcttc ttcgatatct cttgcagtca attcattttc aataccgatg gaaccatgtg
    10381 tttcgacttt tacctcggcg ccaactgctt tccccgcttt ctctaagttt tctgctgcca
    10441 tgtatgtatg ggcaatacca actggacagg atgtgattgc aataattttt ttcattttcc
    10501 taccgccttt ctacatttat agtatataaa aacggtttct ttttattaag cacaaaatga
    10561 ttccgtaata actgaaattc ttggaataaa tagaaatttc gctaaaaaaa ttgatatacg
    10621 atttattatg ttgaataaaa taatattgga aaatcttaca tttctgtact tctgcttacg
    10681 tacatctaaa ttattatcta cattaacaat ttccgtaatt aattataaaa agcttattcc
    10741 atttaatttc attctggaat aagcttctta ttttctaatg agttagtgaa taattttttt
    10801 taccatgctt aatagtatct ttacgtctcc tatacgcgta tctcctgttt ctttatctat
    10861 aattgaacta taaacatgag gaatcacttt ttttacacct gcatcgattg caattttcag
    10921 aatttcttca aagtttgaaa gatcaattcc tcctgttggc tctaaataga attcatattt
    10981 tgcgcaagct tccgcaactg ctttaaattc ttccctatgt gctaagcctt tcatcgggaa
    11041 atacttgatt gaagtacctc ccatgtcttg cagcatttga atggcagttt caataggcac
    11101 ttcagctgct ggcgcttgtg aacttaacgg acctgtagca atattgacat agccgatctt
    11161 acctgttggt gaaaccaatc cattgatgac tgtatcattt tggcccaata aagcacgaga
    11221 agtccccacg ccagtaaata cttgattgac atgttgtggt tgtaaaaccc cagataaacg
    11281 cgaaaccatc tgactttggt ttggatctcc agcgcctaat ccaactgaca acgcattatt
    11341 ggttgcgtct tggtaccttt tcatatcttc gattgctgct tcatcgctag cataattttt
    11401 cgatagcaca cctagtacga catgcccttc cgctgcttca tagcatgctt ttgcattttc
    11461 aactgaatga gctaatacat ttaaacatac acgattttta taataatttg ggataactga
    11521 caatttaaaa gcctcctaca tgatttctct caatcttttt ataatttttt ccatttcgtt
    11581 ttggctgacg gaacgaatgt cgaactcaat gattccgtta tttgcttgat attctcgggt
    11641 gtagactgct ggatttcgtg cttttaattc ctgaatgact tctttcgcac ttttatcgcc
    11701 attaatcgtt acacttgctc gataaatatc tctgccggca ccatcttgaa ctattttcgc
    11761 tgaaaggtca ggaagttgat tgattgcgtt aacaaaaggc gctaatcgtt gcttcatgga
    11821 gtcaccggat tcgctcccat tttttaaata atcttctagt gcctgggtaa agcctaaaat
    11881 attatcttta ccgattttca ttgctcggcc aataccttta gactgtagcc gaatccattg
    11941 aatatacttc ttttttccta tgaccagtcc ggcactcgga ccttcgattg cttttgcccc
    12001 agagtaaatc actaagtcaa cgcccatttc gctatatttg aataaatctt cttccgcagc
    12061 tgcatcaacg atcaatggca aacgatgagc tttggcaact tctaccattt ctgccaccgt
    12121 cagcatactt ttctgaactg catgatgact tttaatatat agaagcgcag cagttttttc
    12181 cgtaatcatc atggaaacat gttccggtgt acacatattt gcatagccag cttctatgac
    12241 ttttcctcca ccttgagcaa ccattacttc aaccggtgtc ccataatcta cattatgtcc
    12301 cttaggtatg atgatttccc gtctagcgat tttttctgta tagggatgat agacatggta
    12361 catgtcgccc tgtccgatta aagcagcaac agattgagca atccctgctg aagcagaaga
    12421 aacgatttgc gcatcttcca ctttcagtaa ttgagaaaga taagcgccgg ttttgatggc
    12481 aagatcactc atctcaaaaa aatgctcgcc tccaaatcgt tgtgctgcta ggacactttc
    12541 agatacctta gaaaccccta ggatcgtcat ttttcccgat gcattaatga cttcttttaa
    12601 atcaaacttt ccatagctaa ttgtcatata tgacgcctcc aataattgtt ttaatcggta
    12661 aaatttgttc tttagctata cgtgtaaatc cgttggaatc tttcaattcc ttttcatttt
    12721 caacaatctc aaatatcgtc aaatctgcat cataattgac agctaactgg cccttattct
    12781 tcaaatgaaa attccgtgca ggaactgctg taactttttc aatgatttct tcccaagaat
    12841 agccgactac cctcaatttt tctaaagtag ttgctagatc aaaaactggg ccatttgtac
    12901 gattgcggat gtagatatcc gtactaatag aggtagcttt cattcctgct tttagtgcgg
    12961 tttctgccac atggaaatta aagctatccg tcccatgtcc aatatcaaag acgacaccat
    13021 tattgtaagc atcccagaca aagtttttaa tttgatctgt ctttggatct aaaataccgt
    13081 ttggttttcc attaaaacaa tgggtcaata catcgcctct ttccattctt gacaatactt
    13141 cttctaactc aggaggtgca gaaccgatat gaaccatcaa tggcagatca ttgttttctg
    13201 tctggatttt tttagctaat tctaatggct taatgccatt ttcaccaaca actgtcttac
    13261 tcattctcgc tttaatcccg acaataaatt ccggcagttc tgctaacgca gtacttacta
    13321 gatcttcttg aatctttgtt agatcagcaa gttcatcttg tttgacaatg ccccatttag
    13381 agatattcat tagcgcatac acatttgtct tgaccgattt cgtcaattga taaaattcac
    13441 ggatgttttc tgctcccgta gttccagcat caatgaccgt cgtcacacct ttttcgaccc
    13501 ctatctgatc aggatagtca taataaagat tcatcttttc atagcagtgg acatgatcat
    13561 cgatccaccc agcggataaa taatactttc cctccaagtc aactacttct tgcgccggct
    13621 gatttatttc tgctgcaact gctttaatca tgcctttttc tatagcgacc tctattttag
    13681 atccatcaac taaccttcca tttttaatca aagtatcaaa catttaatga ccctctttct
    13741 attaatttac tgccgggaag attgctccta aaatcattgc tcctaaaata gcacctccag
    13801 caattggttt tttccaagca tagaatccta gtgctcctaa tgtagcgcca atgccgattg
    13861 gaatagatgc tgcacacgcg cttaatataa ttaatggacc caaaaaacgt ccagatgaat
    13921 ttcctgcccc catcatgacg tcagcaccaa aagtggaatt tgattgattg atcgtaaatt
    13981 tacgaatcaa gacaatgatg ccaccaacaa tcaaacctag aaccaaacca gtcaataaag
    14041 agagtgcaaa attatcgatt ggtgccgcta ttttcatact taagagaatt gctggtaccc
    14101 caattccgat cccagttagg atcgaaccgc ctaagtccaa gatcccaact aaagagcctt
    14161 ctaaaattct ggcaaataag aaactagcac caaacgctgc cgctgcgcca taggatccgc
    14221 catccagccc ggcttttaac atcgccacga tcgaaacttc attgaatgca cccacaccat
    14281 acatcacata catgtgtgtt cctgcaaata cagcagcgga cataattcct acgattagcg
    14341 gaaaagacca gtctgcaaac caaaagctct tttgccgctt tgcttctaat acttttgctt
    14401 gttcttctgt tgtctccatg attctcctcc tatttcacac taaagatgtt gtgcaagtta
    14461 tcaagccaat taggcacttg aagctggaag ctcttgatca tttccatatc aaatccacgg
    14521 aaaaatccac tgaaaataaa taataaaaca atagcgatca tcatgctctt cgtgacgcga
    14581 ttccaaccgc tctcatcgac gcctttacca atcaagattc ctagaacaac accaggtacc
    14641 gcatttccca taatcaattg cgcgatacca ccaaaaagag tgccccaaaa accagttcgt
    14701 ttccctgcgt ctaaagctgc caaccaaaaa ataactggca ttactgtatt gatcagaatc
    14761 gtcgctgctg gtaccaaaac ggaaaccgcc gtcacttgta acgcctctgg aattgctgca
    14821 gctgttgtgt ttaagaaggc taccaccact gccccgataa ccgctccaat gatccccatt
    14881 tttttcggat catgcatggt ctgggtgaca tctttgtttt ttgctagaag tgctgccgct
    14941 gcccaattgg gaatgattcg atgcgtcaca tcttgtgtga atgcaccagc accaactgaa
    15001 gaagcccatg cattaaagaa aaagcctaaa ccaaatgaaa aatgagaagc tggatcaccc
    15061 atgcaggcat tcatttcgcc taatgttcta aaagctccta aaccttgtgt attaggtgca
    15121 tgaaacattc gagctgcccc gacagatgct gcaaaaccaa gtaatccccc gataatgacc
    15181 gatttgacta gaatgattac gaaatccacg ttattctttc ccccttatga ttcaaaatta
    15241 gattttttta gttacaaatg gaatggcaat cccatttgga tcttcagtca tttgctggct
    15301 gaacgatatg tctgccaatt ttatttctgt tacagccaca gtaacagaga gttcgacttc
    15361 atagctaata cgttgccgtg ggaagaagaa aaatagaaag cgttctgtgt atttgttttc
    15421 gaccgccttt aaaatatcca cattgactgg ttcgattcgc aataatacgt ttgattgttc
    15481 cattattttg ttttgaattt ctgctaacgc tgatgcaaaa gcatgttgct ttgtttcccc
    15541 tttaccggag acgataactt tttgctgaat tgtcttttga acgctcattt catgtccctc
    15601 tttctatttt ccatgctttt tgataaattc ttctgtcaga cgtttcccta attcctcttg
    15661 atccataaag ccgaaaccta gaacagtttt gccatccttg atagctgtga tcccttcttc
    15721 aatcgaacgc ataccatgtc gttctggata gccatatttt gttgctgctg ttaatgcacc
    15781 tgcaccacca ctaccacaaa aagagatacc catatctgca tgctcttgtt gcatcacatc
    15841 gcccaaacgc atatctgccc ccattcctgg tacgacaact gcttttccac cagctgcttc
    15901 tacccctttg gccacatttt gtcctttacc catacggtct gcaataacta ctttaatcat
//
LOCUS       DACCCR010000063        15371 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCCR010000063 DACCCR010000000
VERSION     DACCCR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.070"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            4..2376
                     /locus_tag="IVK93_001998"
     CDS             4..2376
                     /locus_tag="IVK93_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387283.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIREEIERFEKGESSFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQSELQAGIQAVANNIGIQIRDITESEIYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKLSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVQQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="IVK93_001999"
     CDS             2437..3897
                     /locus_tag="IVK93_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP6387284.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEAKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLITELKPKHERQNF
                     LVRDNRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVHCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFDQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAILFQQGRQQERE"
     gene            3908..4684
                     /locus_tag="IVK93_002000"
                     /pseudo
     CDS             3908..4684
                     /locus_tag="IVK93_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            4800..5393
                     /locus_tag="IVK93_002001"
     CDS             4800..5393
                     /locus_tag="IVK93_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6387285.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            5409..6308
                     /locus_tag="IVK93_002002"
     CDS             5409..6308
                     /locus_tag="IVK93_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6387286.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERINEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            6311..6796
                     /gene="ssb"
                     /locus_tag="IVK93_002003"
     CDS             6311..6796
                     /gene="ssb"
                     /locus_tag="IVK93_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6387287.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            6841..7074
                     /locus_tag="IVK93_002004"
     CDS             6841..7074
                     /locus_tag="IVK93_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387288.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGRDNSFWRLTKVTIAATAVILTAILPLYM"
     gene            complement(7233..7355)
                     /locus_tag="IVK93_002005"
     CDS             complement(7233..7355)
                     /locus_tag="IVK93_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP6387289.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            7510..7770
                     /locus_tag="IVK93_002006"
     CDS             7510..7770
                     /locus_tag="IVK93_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387290.1"
                     /translation="MDKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            8213..8419
                     /locus_tag="IVK93_002007"
     CDS             8213..8419
                     /locus_tag="IVK93_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6387291.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     QTCGKKYTLSYGDYVLIDEKGKKH"
     gene            8419..8670
                     /locus_tag="IVK93_002008"
     CDS             8419..8670
                     /locus_tag="IVK93_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387292.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            8686..9123
                     /locus_tag="IVK93_002009"
     CDS             8686..9123
                     /locus_tag="IVK93_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387293.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            9568..9747
                     /locus_tag="IVK93_002010"
     CDS             9568..9747
                     /locus_tag="IVK93_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387294.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQKLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            10088..10351
                     /locus_tag="IVK93_002011"
     CDS             10088..10351
                     /locus_tag="IVK93_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP6387295.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            10345..10695
                     /locus_tag="IVK93_002012"
     CDS             10345..10695
                     /locus_tag="IVK93_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6387296.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(10719..11354)
                     /locus_tag="IVK93_002013"
     CDS             complement(10719..11354)
                     /locus_tag="IVK93_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6387297.1"
                     /translation="MQKVGKNLRVAYIRVSSIDQNEQRQVEEMKKFGAERIFIEKQSG
                     ATITHRPVFQEVLDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPI
                     MAEAIGNPLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYS
                     ANAKDPQRRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSTVNHE"
     gene            complement(11674..11877)
                     /locus_tag="IVK93_002014"
     CDS             complement(11674..11877)
                     /locus_tag="IVK93_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387298.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(11887..12309)
                     /locus_tag="IVK93_002015"
     CDS             complement(11887..12309)
                     /locus_tag="IVK93_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387299.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            complement(12478..13668)
                     /locus_tag="IVK93_002016"
     CDS             complement(12478..13668)
                     /locus_tag="IVK93_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6387300.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAVVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQRCLVHIQRNICAKVRVSDRKEVA
                     NEFKEVYRSKSKEEALTSLKKFIDRWKHKYPSMVKSLSNNDYLFTFYDYPESVRHTIY
                     STNLIEGNNKQLKRSFKKKEQFPTEKSEEKYLVIQFNRYNEKNMNHVHRGFGQTTRED
                     WFKS"
     gene            13781..13903
                     /locus_tag="IVK93_002017"
                     /pseudo
     CDS             13781..13903
                     /locus_tag="IVK93_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
     gene            complement(14067..15368)
                     /locus_tag="IVK93_002018"
     CDS             complement(14067..15368)
                     /locus_tag="IVK93_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6387301.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
BASE COUNT         5409 a         2552 c         2980 g         4430 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaatgaa caaatcgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggcgga
      301 ctagatatga cgatcgaaaa tattccaaga atcagagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgt ctttttcagc cgccacgatc cgaaaatatc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt aacgcaatgg aaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca catcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctctgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggagaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga caaccaatac gctgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggatatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatcac tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaactcag ttaaaacctg aagactatcc gaaaatcttc
     1501 ccgaaccgtg tctttgattt tcaattggat caaacagggc agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tatcgggatt caaattcgag atattacgga gagtgaaatc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaactt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 cagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt gcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaagaaag actatctgaa
     2401 aaatggatag tcttttttct tttgggaggt ttgaaaatga cagagaaaaa aatgagtctc
     2461 attgatcgat gcaagcaaat ggatattgtg gattttgcac gaaacaatgg aatggctgta
     2521 gtcaataaag gacgtgacta tcgcttagaa gaccatgatt cgtttgtttt cgatcgaaga
     2581 aaacaacgtt tttattggaa tagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttta tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaagc caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccacttacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaattgcc agagtggctg attgattatg cagaaaaaga aggattgatt
     2881 actgaactaa agccaaaaca tgaacggcaa aattttttag ttcgagacaa tcgtttagat
     2941 catgcagttg cctttttatg gaaagatcca cagacaaaag agactgtggg ggcgagttat
     3001 caaggaacgt ttattgatta tgaacgtttt ggtgagagag gaacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tacgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatcgattga gatttgtgtg
     3301 gataatgatc gtgcaggaca tattttctac gaaaaggaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acattgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtca atgcgtcttt tgaccagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aaggttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtga aaaaggattg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat attgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaaaccaccc gaaaagacgg gtattttgag atccaagatc gattgttttc
     4021 gggagaaact gtgatcacgt atggttttgg tcatttagtt gagttagatt cgccagatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcgctatcat gttcctaaag ataagaaaaa acaatttaac gtagtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gtgcagacca gggaaaaacc tataaaagac tttggatcaa
     4321 ctccttagaa aaagaagcga tttatcaggg attccagcag ttacgagacg ctggagagac
     4381 ctatccaaaa tttgaggaag ctcaagcacg tcagatcgct gattggttga tcggcatgaa
     4441 cggtagtcca ctatatagtt tactcttgca gcaaaaaggc atatcaggaa gcttctcctt
     4501 aggcagagtt cagactccaa cgctttatat gatttatcag ttgcaagaaa agattaggaa
     4561 cttcaagaaa gaaccctact ttgaaggaaa agcccaagta accgctcaaa atggcacatt
     4621 tgatgccaag cttgatccca atgaaaccca agtgacgcaa gaagcatttg aagcgtattt
     4681 aaagaaccag cagcggattc aaaaagaaat tgaattagat cgataaattt ctttcttgtt
     4741 aatataaaac taacatgtta tagttatgtt agttttatat taactttagg ggaaaagcaa
     4801 tgaaagacaa aatccagtgt atttttgata aacataatgg gaatctatcc ttgaaggatg
     4861 ctagaagtga aggattatcg cctatgacgt tagatcgatt agccgaaaag gacaaaatag
     4921 aaaaggtagc accaggtttc tataccttaa aagaagcgtt cgtggatgaa ttgtatctcg
     4981 ctcaaagtat ctattccaga gggatttttt ctcatgagac tgcgctcgat ctttttcaag
     5041 taggaacgta tatccctaaa aagatcaata tgatgtttcc taaaggatac aatgtttcaa
     5101 aggagaaatt agacgagtat gctattcaac cgcattacac gaataaagaa aattttgacc
     5161 tgggaatcac agaaacagaa agcttttacg gcaataaaat tttagtctat gatttagagc
     5221 gaacgttatg tgacatgtgg aatccaagat acaaagcaag cgtagaggta aaacaagaag
     5281 cattaaaaga atatatgaca agtaccagta gaaatacaaa taagctaaga agatacatgg
     5341 atattttaaa atcaccaaaa gaaatgactt tgtatatgtt gccactttat tgagaggagt
     5401 aaaggtgaat gacaccgacg caatttaaag caaaaacgag aaattttgca acagagaaac
     5461 atttaaaccc acaaattgtc caaagatatt atatgatgga aaaattatta gaacgtattg
     5521 ttgaatcgcc ttatcatgat gattttgtat taaaaggggg atttctgatc ggatcaaaat
     5581 acgggttaga aaaccgttca actgttgata ttgatacgac ttatcgaaat tcgaaactaa
     5641 caaaagaaaa gttagaaact gttttttctg aattaacagc tgatcccaca aaagatggaa
     5701 ttcgatttga attaaaggga ttaacagaga cgagggaagc tgattattac ccaggttttc
     5761 aagcaaagtt tattgcttat cttgagaatg cacgtgtacc atttaaatta gatattactt
     5821 caggtgattc tattattcca gcggcattga tgtatgaaca caaattaatg tttgaagata
     5881 aaacgattca tattccagct tatcctacgg aacaaattct agctgaaaaa ttacatgcca
     5941 cgtttagttt tggaaaagat aattcgcgca tgaaagattt ttatgacctg tatatgattc
     6001 cgaaattaga acgtatcaat gagaaaaatt tgtataaaag tgttcaagga acattcaagg
     6061 aaagaaataa tcaatcggtg tttaagcatt tgtatgacca ggaaatgcct caattggtga
     6121 aggatgaggg gttgagggaa aagtggaaaa gataccaaca agaaaactat tttgccaaag
     6181 acatttcttt cgaatctaca ctaacttccg ttgattccct gatgcaatca attgtagagc
     6241 aagagaaggt agaacgaaaa caaaatttta agagatcaca acaaatgaac caagaaatgg
     6301 agagataaag atgattaata atgtaacttt agtaggacgg ttaaccaaag atccagattt
     6361 acgttatacc gcaagtggca cagcagtagc gacttttacc ctagccgtca accgaaattt
     6421 cgcgaatcaa aacggcaatc gagaagccga ttttattaac tgcgtgattt ggcgcaaacc
     6481 agcagaaaca atggcgacat tagctaaaaa aggcggcttg atcggtgttg tcggacggat
     6541 tcaaacacga acttatgaca accagcaagg acaacgtgtg tatgtgactg aagttgtagc
     6601 agataacttt caactattag aaagtaaagc agcgacagag agccgtgcgc acgctgatca
     6661 atcgtccacc agtccaagca ccgccacctt tgaacaacgg gatacagcga cgcctaacaa
     6721 caatggattg aacgcttcac agaatccatt tggtggacaa tcaatcgata ttagcgatga
     6781 tgatctaccg ttttagttcg agaaagaagg agtaagcatg gcaaaaaaaa tagtctgacg
     6841 atggatctat tgatacccgt aattgcattt atagacggga tttctttggg cgtcactgaa
     6901 aatcctttgg taaatagaat tgcgaaagga tcactatttc ttgtattcgt gtatcttgta
     6961 tgttcttatc ttttaaaaag agcaggaagg gataattcct tttggcgatt gactaaagtc
     7021 acaatagcag cgacagctgt tatacttacg gctattctac cgctttatat gtagggttaa
     7081 cgggaaatcg ttgaacttct tgcgaaatat tgctataatg aacatgaaaa agaggagttg
     7141 cgctaacaac ccctcattaa cagcaccgat taaagaacgg tggcagaata ttagttattt
     7201 ggaaataacc gctgacctgc taaagtggac ggttatttct ttttgtcatt tttaagcata
     7261 tttacaatca aactaatcaa agcaatggta aacataccaa aaccaagaat ggtttgaatt
     7321 gtctcatatg cggacaaaag gcttactcct ttcatagatt ttagcactta cattcataag
     7381 caccaccacc tttccagaag tcgccaccgt cttcaaactt tactgtcttg actattatag
     7441 catatctaaa atcgaataaa aattctcaat tttggttgtt tttgctataa ttttagcgag
     7501 gtgattgtga tggacaaata tgttggtttg cttgataaaa tccgagtaat aaaaacacaa
     7561 ccattattgg ttcgctttac tttacaaaca attcatgaat cgattaattg tgtagttgca
     7621 gatattgaaa ttatcgataa actgctaatt atggatgatg gaaaatacaa tattgccgtc
     7681 acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt tagaaatccc
     7741 gatcatttta cgcgatcgat gggcatataa aagaagagtt gtagtcctaa atgaggattg
     7801 cagctctttt tgttttacat agaaacggga acaggaaaag gcctgtttag gaaggcacaa
     7861 gcgccttctc aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa
     7921 aggaagtgaa cgttgcacgt tcactaaaat aaggggtctt gcaaagcaag acaaaatagg
     7981 aaaaacttcc tctcaccaaa aggttcgttc ctgttcgcta tctctttatt gatttgccag
     8041 accggcaaca agttgccttt tatcaccaaa aagcgacaag gacaagggta aatggcacgc
     8101 tgcgcgccct tgtccttgtc gcttttcgtt gattcattct ggccaacaaa tcaataaaga
     8161 aagaactaac aaaaggaagt aaaccctttc ctaagaggag gcagaatcaa tcatgcgaaa
     8221 gaaatttgat tattggggtg ttcccttcag cgaattattc ccaaactatc atgcaccaca
     8281 tactgtcgaa tgcgattgtg gagaaagagc gaaatgtatc aaaagctatc gtttgtatca
     8341 atgtcaaaca tgcgggaaaa aatatacctt gtcttatggc gattatgttt tgattgatga
     8401 gaaaggaaag aaacactgat gaaaacacaa cacaaattat tgactgtaaa agaagtcgtt
     8461 caaattttgg tggacaaaga agtcatttct gaaaaagtag cagaagcttt acttattccg
     8521 cggtactgga cgatagaaac ccctttagta gaggatgcac cgactatgct atttgaagac
     8581 ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa acatggaaac ttatgaaaac
     8641 gaatattggt taaccaaatt agagaactaa aaaggagaaa aaaatatggc tcaaaattac
     8701 tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg
     8761 ttcctataca acgaaacacg tgttccaaag aaacattata aagaaaaatt gtcggtagct
     8821 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ctatcgaaca
     8881 ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa
     8941 gtaggcgtca aaccaagcac aatcaaacct tctgaatacc aagctttaga actaacgtat
     9001 gcgaaattta tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt agattatttt
     9061 gaaaatatca ataagtatgg cgcatattac accatgaaaa aggaagacaa cgaaaatgaa
     9121 taaggtgatt tatcaactaa caacggattc catatcaacg atttgaaaga aaaaaaggtc
     9181 aataatcatt tataccgaaa agtgagaatg tgtgaaataa ggacaaaaaa agcctagtaa
     9241 ctaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtgat
     9301 gcgaaataaa cgggcaaagc ccgataactc aagagaaaga aggtgatcca tttgaacaag
     9361 attattccct tattgttagt actatttgtt ggcgaaataa ttatgcgaca tagtgcagct
     9421 gtgcatttaa acttcgtagt atttttggta atactggtgt tttgtttgat cttttggcgc
     9481 tttgtgttag cccttgtgag tctaatattc aagttgattg cagccatatt tttcataggg
     9541 ttatttcgat gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc
     9601 gttacgttgg atgaaaagag acaaaaagaa ttgcagaaac taaagcagaa gtacggaaaa
     9661 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
     9721 tttgatgtac cagcactcaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata
     9781 gcacttaatt gttcctacta tttagaatat tttctatatc ctttttattc cattttgtat
     9841 caataactat gaaatcttta aagtatccag aagagattat ttttagatca atttttttct
     9901 tacctgattc taaagatttt gttttcttaa acatattatg atttcctttc gactattttc
     9961 atataaatgt taacatagtt tatggattca tggaatgtta atctaattta atagaaaagg
    10021 ataattaagt atatacgttt tttcttatgt atgttatact atgtatatac agaaagaggt
    10081 gtctactatg gaaatgttac ctataaaaaa atggggaaat agtaatggcc ttagattgcc
    10141 aaagtatatt atggaatatc ttggaattca tacagaagac aaagtgaaga taatacaaga
    10201 agaagtaaac ggtcagaagc ggctaattat cgaagcggct aatatagaaa atgaattaac
    10261 aattgaacag ttatttgaaa attacactga tgaaagaaat catgtgacga ttcaaaattt
    10321 aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta gacaaggcga tattctatta
    10381 ttgaatacag cacctcgatc tggacacgaa cagacaggta aaaggcctta tattgtttta
    10441 agtcatgata ttattgcgga ttatagtaac gttgttacag ttgctccaat ttctagtaca
    10501 actagaaact atccgctata tgtaaatata aactcagaat atgaaatgaa gacaacagga
    10561 aaagtattat tagatcaact aacgacaatt gattatgaag ccagagagtg tatttttttg
    10621 gaaaaagcgc atgatagttt agttgaggag ttattgatga aagttagaac agtttttcaa
    10681 aaagtaagca aataaaatag aaacagcagc atgatcaact attcgtgatt gaccgtgctg
    10741 tcttttttta ttctatatac ggtttgtctc gtaacattgt agtcttttgc aatcttagcg
    10801 atcgcaacac ctttttttag atcctcaacg atgtttttat aaacaagacg acgttgggga
    10861 tcttttgcat tggcactgta tagcttagga cgtcctttat aaacaccgtt cgctttcgca
    10921 atcttaatgc cttgtgcctg acgtcgttta gattcggtac gttcttgttc agcgatcatg
    10981 gccaaaattt gaatgatcaa atctttaatg aatttatcca ggagaggatt gccaattgct
    11041 tcggccatga tcggtagact tgtaatgatc agctgcacat ttttcttctt caagtaattg
    11101 actgaagcga tgatctcgtc gtaatttctt ccaagtcgat caatagcttc cacaataaaa
    11161 atatcttgat ctcggacgta atccaatact tcttggaaaa caggtcgatg agtaattgta
    11221 gcgccagatt gtttctcgat gaaaattctc tctgcaccga attttttcat ttcttcaact
    11281 tggcgttgtt cattttgatc gatcgatgag acacgtatgt atgcaactct caaattttta
    11341 cctactttct gcaactattt aaaacacacc ctaatcatac atgagaaaac cagatttttc
    11401 aatgtttgtc atttttctag ataaatggca taaattttca gcgctaagtg acaaaagcaa
    11461 ctaatttttc atgatagatt tttaaatatc tcaatctatc tccaaaatta gttgcttttg
    11521 tagctatagt tgactatgaa tatgacaaat aattagaaaa aactgtgcca attaaatata
    11581 aaaatgacag aaagtgtcca gaaatgggtg attttatgaa cactttcaat tagaaaaaaa
    11641 gaatgtcgac tagacactct ttttttaaaa tcattatttt tctttttgat atgttttttg
    11701 gtctcttttt tttctttttt cttcagaatc ttctagatta gaaattgatt tttgaatttt
    11761 ttgtgtctcc tgtaaagttc gctgaacttg taaagctgat tcttctttcg gtaaattaat
    11821 tttacttaga atatcgctat cttgataaaa atttggtgtg ttcacaaatt ctggcataaa
    11881 aaatccctac tttctagtta aggttcctga ataagtcgca atccaattgt ttccagctga
    11941 tttataactt tgtaaataca attttccata gtaccaataa ccatttcttt tttctttcca
    12001 attccgtgat tgaggaggtc tagaaggcca accaacttta tcaaaaatca tcgttcctgt
    12061 cactcgtatc gttttagaag ctgctcgagc aagtaatgga gaaactatta aataattttc
    12121 agtaacagtt aagtcgttag ttgggttacc aatggctatt ccgttcctat ctaaaggagt
    12181 taattcttgg ttattatcta ctacagtagt tggttcattt gtactatcag caaatgtagg
    12241 aattgtagtt ccagttacac taattccaat agttaaaata cctaaagata aaataaactt
    12301 atttttcatt gtctatcacc tcgaaatcaa aaataagaaa acgttttctt aataaatctt
    12361 aacagataaa tcaagttata tactagaaaa ttcataaaat atatttttaa tgtcataatc
    12421 gtttaaattt gggagcgtca ataattttgt gtaaaagtat atccttggta aagcagatta
    12481 gctcttgaac caatcttctc tggttgtttg tccaaaacct ctatgtacgt gattcatgtt
    12541 tttctcgttg taacgattga actgaatcac aaggtatttc tcttcagatt tttccgttgg
    12601 aaattgttct ttctttttga aacttcgttt taattgttta ttgtttcctt caatcagatt
    12661 tgttgaataa atcgtatgcc taacactttc tggataatca tagaatgtaa acagatagtc
    12721 attgttgctg agacttttca ccattgatgg atatttgtgt ttccacctat cgatgaattt
    12781 ctttagagaa gttaaagctt cttcttttga tttggaccga tatacctctt taaattcatt
    12841 ggccacttct tttctgtcac tgactcttac ttttgcacat atatttcttt gaatatggac
    12901 caggcatctt tgaattttag agcccgggaa aatgtcctgt attacatttt ccatcccaga
    12961 caatccgtct gtacaaaata aagaaatacg ttcaacccct cttgatttaa ggtctttcag
    13021 aagttcttcc catatctcag cggactcgtt tggtgcaatc atatagccaa gaatttcttt
    13081 tgtaccttct actgtgattc ctagtgctat atggaccatt tccctggata cagtatcacg
    13141 gcgtaaaggc atggatgttc catcaagata tacaacggca tactctttgt tcaatattct
    13201 gtttttgaag gaatctacac attccagagc tttgtccgta atattagata tggtctgttt
    13261 agaatagcga gctccacata atttttggat catggaacaa atttctgaat ggctcattcc
    13321 ttcttcaaat aaatccaaaa tcgttgtttc ggtaaataga tctcgtcttc tgtatttagg
    13381 cagaagagaa gtgaaaaatt ctcccattct atctcttgga atattgagtg taatagttcc
    13441 atattttgta tcaaactttc tttgatagga accatttcta gaattttcgt tattggttct
    13501 agcgtatggt tcataatcaa gaaaatccgt caactcatat gcgagtattt cattgagaga
    13561 tttctccaac tctgaacgaa acagttcggt tacagcctgt tcaaaagctg gaagattgtt
    13621 gtcattagaa aataggtttt tcattaaaga tgtagtaaaa tagttcatgg gtatattcct
    13681 ttcgtaaatt gtttttggtc gaactaattt tactaaggat ataccttttt ttctacattt
    13741 ctagaaaaac gaatttacac aagatatttt atgctatcca tttatttttg aaactccatt
    13801 tgttttacta cgtgtattac ctttattttt ctttctattt ttaggtgtct attatctctt
    13861 acgttcgctt ataaatgatg actcaaaatt taaagcaaaa taaaaataaa ggaagtagcc
    13921 ttttgaacaa gggcgcttcc tttatttgtt gtgggacacc agagtatact tttttataaa
    13981 aaataggttg atgggaaacc ctaagatttc ccatcaacac taaatagtat agaaccatat
    14041 tttcgttaca aggttaagaa tgacgactat agtttttcat ctactaattt tgttttattg
    14101 ctctccatga ctttaaaaaa tggatagtag atgaagatgc tgatgacgat caacagtagg
    14161 caaaagagaa tattgcgcca gtccatactt gaaagataag cttgtgcaaa aaaaggtgtg
    14221 aaggaaggat cgacgataaa tccaatccca atccatccta gttgctgagc aaagtaggtc
    14281 agcaataatg aaatgatggg cgtaatcaaa aatggaattg ccaaaatagg gttaaaaacg
    14341 ataggtaatc cgaaaatcac tggttcgttg atggaacaga ttccaggaac caatgacaat
    14401 tttccaattg tgcgatattc gggtactttg cttcgcatca tcaataaaac caaacccaaa
    14461 gtaccaccgg ctccaccaag tacagaaata cggaacattt gtaagttcat taagtgtgtc
    14521 atggtttcac catttgccag ttgttcagca ttcaaaccag tttgagccat tcccaatgta
    14581 aagacgatag ggaaaataat tgacgagcca ttgataccaa atagccatag gatgttaccc
    14641 aaagtcacga tcaataagaa accacctaga ctgccagcga tgttcgttgc tggagtcagg
    14701 acttccatca ccgcttcagg gaagattttg tttgttgctg ccaagaaaat cagattgatg
    14761 ccataaaaga tgaatatatt tgtcagcagt ggaacgagca tattgataaa ggttgctacc
    14821 atgggtggaa ctgtatctgg gagtttcaac ttaatatttt ttacttcaaa tagtcggttg
    14881 atttccacta ctagcaagcc gatgataatt gcgacgaaca atccgtttgt cccaaggaaa
    14941 ttaagattga tggcaccttc ttccactggt gtacaaacca ttagataaat gacagatgca
    15001 accatgccat tcatggaagg attaattttg tattcattgg caagggaaaa tgcaatgcca
    15061 aaaacactaa tgaatccgat gattcccatt gtcaaattat aaggggctgt gatgagatca
    15121 taatttgcta cggcaaaatc tttccagctg gccataaatt tcatgaaaat attagctgta
    15181 tctggattgt atttatcaat attgatgggt ggatttgcaa aaatcagaaa aaaagaaccg
    15241 ataacaataa agggtaggcc aaacatcata ccgctggata tagcttttag gtggcgctga
    15301 ttacctatct tggctgcgat ggggctaagt atgtcattca gtttcatgat aaacgcagaa
    15361 ttttccatat a
//
LOCUS       DACCCR010000064        15313 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCCR010000064 DACCCR010000000
VERSION     DACCCR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.071"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            278..787
                     /locus_tag="IVK93_002019"
     CDS             278..787
                     /locus_tag="IVK93_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6387302.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IVK93_002020"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IVK93_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6387303.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IVK93_002021"
     CDS             2322..3302
                     /locus_tag="IVK93_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6387304.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IVK93_002022"
     CDS             3424..4842
                     /locus_tag="IVK93_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP6387305.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IVK93_002023"
     CDS             4913..6382
                     /locus_tag="IVK93_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6387306.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IVK93_002024"
     CDS             6392..6859
                     /locus_tag="IVK93_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6387307.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IVK93_002025"
     CDS             6895..7767
                     /locus_tag="IVK93_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6387308.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="IVK93_002026"
     CDS             7849..10383
                     /locus_tag="IVK93_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6387309.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="IVK93_002027"
     CDS             10539..11312
                     /locus_tag="IVK93_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6387310.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="IVK93_002028"
     CDS             11305..12096
                     /locus_tag="IVK93_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6387311.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="IVK93_002029"
     CDS             12106..12597
                     /locus_tag="IVK93_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6387312.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="IVK93_002030"
     CDS             12615..13025
                     /locus_tag="IVK93_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6387313.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="IVK93_002031"
     CDS             13029..14429
                     /locus_tag="IVK93_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP6387314.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="IVK93_002032"
     CDS             14452..14826
                     /locus_tag="IVK93_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6387315.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4782 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttc
//
LOCUS       DACCCR010000065        14838 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCCR010000065 DACCCR010000000
VERSION     DACCCR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.072"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            418..1032
                     /locus_tag="IVK93_002033"
     CDS             418..1032
                     /locus_tag="IVK93_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6387316.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1805..3544
                     /gene="yfcC"
                     /locus_tag="IVK93_002034"
     CDS             1805..3544
                     /gene="yfcC"
                     /locus_tag="IVK93_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP6387317.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3701..4885
                     /gene="iadA"
                     /locus_tag="IVK93_002035"
     CDS             3701..4885
                     /gene="iadA"
                     /locus_tag="IVK93_002035"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP6387318.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            4996..5199
                     /locus_tag="IVK93_002036"
     CDS             4996..5199
                     /locus_tag="IVK93_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6387319.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5300..5962)
                     /locus_tag="IVK93_002037"
     CDS             complement(5300..5962)
                     /locus_tag="IVK93_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387320.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5975..7477)
                     /locus_tag="IVK93_002038"
     CDS             complement(5975..7477)
                     /locus_tag="IVK93_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387321.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8025..9704
                     /locus_tag="IVK93_002039"
     CDS             8025..9704
                     /locus_tag="IVK93_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6387322.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9831..10310
                     /locus_tag="IVK93_002040"
     CDS             9831..10310
                     /locus_tag="IVK93_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP6387323.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10529..11209
                     /locus_tag="IVK93_002041"
     CDS             10529..11209
                     /locus_tag="IVK93_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP6387324.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11217..12548
                     /locus_tag="IVK93_002042"
     CDS             11217..12548
                     /locus_tag="IVK93_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6387325.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12563..13525
                     /locus_tag="IVK93_002043"
     CDS             12563..13525
                     /locus_tag="IVK93_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP6387326.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13636..13974
                     /locus_tag="IVK93_002044"
     CDS             13636..13974
                     /locus_tag="IVK93_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP6387327.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14053..14760
                     /locus_tag="IVK93_002045"
     CDS             14053..14760
                     /locus_tag="IVK93_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP6387328.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4761 a         2578 c         3192 g         4307 t
ORIGIN      
        1 tttttttgta ttttcttgct tgcacttgga taagagatta cttacaataa attcagatag
       61 aaacagctga ataaaaaaag gctccctgac ccttagttgg cgctaagaat cagggaatga
      121 ccagtcacac acatacaccc tgaccgatca ttttgccata gtcaatgcag tgcatcgacc
      181 taatttattg tacctaatct agggtgaaaa ttctacccct atgtagatat tttccatggt
      241 ctgcacgttg ttttatttgc tatgtcaagg tattgggcgt attcgttcaa tgcctttttt
      301 gtgtttgaaa cagcaaagac cacatgtgtt actgtctaac tactgttttt ttatgtactt
      361 ccttagatta ggcagctgtt tctattcatg tcaagtatag ctaggcggga gagataaatg
      421 agtttatgtt attttgacta ttatcaccaa ttggaagaga aattaaggat agaagagccg
      481 ctgatgcttc aagctggcac ttggatcgcg aaacaaatta atcaaggtgg tagattgcag
      541 atatatgcta gccgcacact aagtggtgta gcatttgaat tttgggatca aaccccggat
      601 cttgtaccaa gtatattgat tgaaaatcca gccggaggtg tctatgaaac gctagaaggt
      661 gcaggacagg caattattga tcagctttct gtcaaatcag aagacatttt tcttcttctt
      721 tcgaatgaag gaagaaaccc ggcaatcatc gaactagctc agtggatcaa agaaaatggt
      781 catccattaa tcattgttac tggatttgat ttgtcacgaa gtatcaaatc gcaacatagc
      841 agtggtttgc gattatttga atttgctgat ttggttttgg acaaccacgc caatttacat
      901 gatgcagtct tgactttgcc aaatctagat ccagcgatct gcggaacagc ctctcttgca
      961 acgatctttt tgctgcagca agttttgtat tttacagcaa aacagctagt ccaggacaca
     1021 agtggaaaat agttatccac atgttgacaa agttccttaa aaaaataatg ttccacataa
     1081 aaatggagcg tttgaaaaaa agaaaacgat tattaatgag gattaacaac gactactttt
     1141 tttgttttag ttattttaag atggatcatc tttgatttta agcttgtttt gtttaaagtt
     1201 tgttattttt ataagttgtt tcacagttat gagaaatgag ttataagaat atgtagtgtc
     1261 agaaaaataa aaagtagtgg aaaaagagac aagaaagaat ccatatggat tctttcttgt
     1321 ctcttaatac acgttgatga aagagtcttc cccaaaactc tttacaaagt aattatagta
     1381 catttgttat cattattgca atgaagttta cctgatataa caaaattgtc caacaggata
     1441 cctttttcta atagaaataa aatacacaaa aattgaagga tagataatgg atatttataa
     1501 gtgtttatta ttttttttgc aatgaaattc aatttttcaa tgattgtaaa atgaaaaaaa
     1561 caggacgaga ttttctctaa tctaaaaata aaaaaagaat tttaaggaaa aacagagaaa
     1621 taaaatactt aatataaatg gaaagagaaa aaaataatca tacataggag aaaaaaaaca
     1681 aaatagaaaa aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat
     1741 aattcatttg attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat
     1801 aaatttgaag aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac
     1861 accccatacg tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt
     1921 cgttccagca ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac
     1981 aagtactcgt actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc
     2041 gtatgtgttt gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga
     2101 tgttccggaa aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga
     2161 aaaactagat gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat
     2221 ctatgatact tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg
     2281 cggctttggt ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc
     2341 tgctgtaggg atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg
     2401 taccggagcg atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg
     2461 tttagcttta ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc
     2521 agaagaggta attccattct caatggtgat ggttccattt gtcattgcgt taggatatga
     2581 ctcgatcgta gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg
     2641 gatgagcccg tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg
     2701 tgcgacattc cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat
     2761 gatatatgca gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga
     2821 ttatttccgt tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca
     2881 taaattgatc ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca
     2941 aaaaggctat tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg
     3001 aatcattgct gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt
     3061 ccaatctggt gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat
     3121 tcttcttgtc ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca
     3181 tgggatcggg acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat
     3241 cttccaaagt ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat
     3301 gccaatcatg gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc
     3361 ttatcagctt ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg
     3421 tgtgctaggt gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca
     3481 aggattcctg tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca
     3541 gtagtactgg agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa
     3601 ccgtcatttt acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag
     3661 atcctatcat cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat
     3721 tgaagtgttt gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat
     3781 tttggctgta gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc
     3841 aggtgaggga aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg
     3901 aggaggagaa ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac
     3961 agctggtgta acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac
     4021 tgcgttgatc agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga
     4081 aggttcgtac cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac
     4141 aattgataaa attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc
     4201 aagttttgaa gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg
     4261 aaaagctggg atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac
     4321 acgaatcgta gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg
     4381 cacaccagaa ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt
     4441 cactgctagc gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa
     4501 agcactgaaa cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga
     4561 tggacaagga agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg
     4621 aagtgccaag gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt
     4681 ggaaatcgcc ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa
     4741 aggaaaaatc gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga
     4801 tattgataca gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg
     4861 ggggactttc gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc
     4921 gttctgtttg tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta
     4981 agaggaggaa gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta
     5041 gtcggtgaag gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa
     5101 gggaaagcac aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa
     5161 gtcaaagaca agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga
     5221 atcaagcaaa taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat
     5281 ttgatcatta tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa
     5341 agacttcatc acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat
     5401 tttgttcatg ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta
     5461 gatttccagt aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga
     5521 tagcgactcg ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt
     5581 catcaattcc tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg
     5641 tcttagtgat ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt
     5701 tgtaagcttg gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt
     5761 tcagtgaacg atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa
     5821 gcgaaagaaa agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag
     5881 cttcgaaatt cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc
     5941 ccaattgttc tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt
     6001 ttaatcttaa tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta
     6061 ctccggctaa tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat
     6121 cagaaacagc aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt
     6181 cttgtctgtt acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt
     6241 ctgtctgttg gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg
     6301 aagcaactcc taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga
     6361 ttttacttct ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa
     6421 tggtaatcat aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa
     6481 catttttaga aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat
     6541 catttgattg taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact
     6601 catccatttt atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag
     6661 atgtcccatt tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca
     6721 tgaagttgaa tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga
     6781 tcgtcatttc ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga
     6841 ctgccaggtc atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt
     6901 cgctgtcatc tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat
     6961 tttgtgcagt tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc
     7021 cgcccattcc tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg
     7081 aatcagaact agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa
     7141 aggaataact ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg
     7201 gtgtaagaga aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag
     7261 tttgttcctg ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa
     7321 cttctttttg tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta
     7381 gcacaaaaat aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta
     7441 cttttgtact tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat
     7501 gcatttgata atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc
     7561 atcaacatga ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa
     7621 aatgaaaaaa gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa
     7681 tcctagatgc tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag
     7741 cgatttagtt tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct
     7801 ggctatagcg tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc
     7861 catccaactg tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga
     7921 gaaatagtga ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa
     7981 atcgacatct ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa
     8041 ttatcccatt aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg
     8101 aggatatttt tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg
     8161 acgtcgtaat ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct
     8221 ttttaacaca tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt
     8281 ctgtaccagt attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta
     8341 atgaagcagc caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact
     8401 ttggtgaaac agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga
     8461 tcaatctgaa aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa
     8521 cggctatccc aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag
     8581 tcttagcctt actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag
     8641 cccaaatagc agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg
     8701 cttgtgtagc aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac
     8761 aacgaaaagt tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt
     8821 tagaaaaact tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact
     8881 atgatccaga agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc
     8941 tgcaaaaaat ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt
     9001 atatcactac tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca
     9061 tttatcgagc tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg
     9121 ccaatcctaa tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag
     9181 ttcaaggaga gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc
     9241 catacaaaaa tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta
     9301 tgagtgttgc aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg
     9361 atatcggaaa cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag
     9421 cggttgtcac tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac
     9481 ggggattcgt ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg
     9541 aaacaatcgt tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag
     9601 acatccgaga tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct
     9661 tgccagtcat tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat
     9721 gaacctctct atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa
     9781 tacagcatcc ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc
     9841 aaacaaaagt cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta
     9901 gtcatgtggt agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt
     9961 tgatatgtaa aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc
    10021 ctttgaacga tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg
    10081 gttttcctgg aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc
    10141 ctattggaat aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag
    10201 aaaaagaaga agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag
    10261 ccgatttaga aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa
    10321 gagaagctaa gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc
    10381 atcctgtatt atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata
    10441 tatttagcag ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa
    10501 gatacagaga aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt
    10561 aacaattgaa atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc
    10621 attgacagac ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca
    10681 tagtgaccac ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag
    10741 tattgttgag attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat
    10801 cggtggaaaa catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc
    10861 aagctatgaa gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca
    10921 agcagaagga aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt
    10981 gcttgcagaa gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat
    11041 ggggccagaa gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat
    11101 ccattacaat actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga
    11161 agatagtgaa ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg
    11221 cgacaaaaca tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt
    11281 ctgtaagaag catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca
    11341 aagatgcaga aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg
    11401 aacgcaaatt aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg
    11461 aaggggacgt tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg
    11521 gcgcgatgac taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg
    11581 gcaaccgggc ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag
    11641 gaggattcaa tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct
    11701 tacgcacctc ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc
    11761 aattgatcaa aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc
    11821 attatttgca gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc
    11881 attcgcgttt tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag
    11941 atgttttagg gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca
    12001 gtgtaaagaa aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag
    12061 aagtcatgcc ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg
    12121 taatgaaagc gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc
    12181 agatatctgg aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc
    12241 ctaagttcaa attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg
    12301 gcgtacttag cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac
    12361 gaaatatctt gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg
    12421 aattagacat tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg
    12481 aagtgtttat tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac
    12541 gttcttagaa agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa
    12601 atacgataaa atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca
    12661 agtaggatta gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg
    12721 tgaaacgatt gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta
    12781 ccaaggagcg ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag
    12841 atatcagttg gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg
    12901 agatcttgtg tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc
    12961 tttgatgttt cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg
    13021 aatcgtcgga gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat
    13081 tgcttcgata ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca
    13141 aatgccgatg aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga
    13201 aaatggtgca ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga
    13261 ttcagagaca gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg
    13321 gatttttgtt cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat
    13381 catcaatgaa ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa
    13441 tgcaaaagat ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc
    13501 gtataagagc agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa
    13561 tcacaaatgt atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt
    13621 taaaggagaa agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat
    13681 gaagatcgag gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa
    13741 gaaactgtca tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag
    13801 ggcgatagcg tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa
    13861 caaggaacaa aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag
    13921 gtagaaggat ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata
    13981 ttcaaggtct tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg
    14041 agtgaaacga aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg
    14101 gtcattcttg gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga
    14161 aaatacgatc aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata
    14221 ttacaaagtc aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat
    14281 aactacaatt caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca
    14341 gttgctgcga taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt
    14401 tatgtcccat tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt
    14461 tcgttcccct tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc
    14521 gggattttcg ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt
    14581 aacgcgtctc ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa
    14641 gtaccgatgg caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt
    14701 tcgacattcc tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa
    14761 aataaagaag ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg
    14821 aataccattt atttggaa
//
LOCUS       DACCCR010000066        14549 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCCR010000066 DACCCR010000000
VERSION     DACCCR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.073"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            4..816
                     /locus_tag="IVK93_002046"
     CDS             4..816
                     /locus_tag="IVK93_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP6387329.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IVK93_002047"
     CDS             813..1613
                     /locus_tag="IVK93_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP6387330.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IVK93_002048"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IVK93_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP6387331.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IVK93_002049"
     CDS             3110..4819
                     /locus_tag="IVK93_002049"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP6387332.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="IVK93_002050"
     CDS             5031..9389
                     /locus_tag="IVK93_002050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP6387333.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="IVK93_002051"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="IVK93_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP6387334.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="IVK93_002052"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="IVK93_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP6387335.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="IVK93_002053"
     CDS             11227..11520
                     /locus_tag="IVK93_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP6387336.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="IVK93_002054"
     CDS             11517..11828
                     /locus_tag="IVK93_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP6387337.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="IVK93_002055"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="IVK93_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP6387338.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="IVK93_002056"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="IVK93_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP6387339.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DACCCR010000067        14233 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCCR010000067 DACCCR010000000
VERSION     DACCCR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.074"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            221..1494
                     /gene="efmA"
                     /locus_tag="IVK93_002057"
                     /pseudo
     CDS             221..1494
                     /gene="efmA"
                     /locus_tag="IVK93_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
     gene            complement(1644..2243)
                     /locus_tag="IVK93_002058"
     CDS             complement(1644..2243)
                     /locus_tag="IVK93_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP6387340.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2647..3768
                     /gene="mnmA"
                     /locus_tag="IVK93_002059"
     CDS             2647..3768
                     /gene="mnmA"
                     /locus_tag="IVK93_002059"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP6387341.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNHAEVVFD
                     EPVRAITPGQAVVFYDEMECLGGGLIDHAYQETKVLQYV"
     gene            4014..5435
                     /gene="arcD"
                     /locus_tag="IVK93_002060"
     CDS             4014..5435
                     /gene="arcD"
                     /locus_tag="IVK93_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP6387342.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFAIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVAFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5449..6567
                     /locus_tag="IVK93_002061"
     CDS             5449..6567
                     /locus_tag="IVK93_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP6387343.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6627..7919)
                     /locus_tag="IVK93_002062"
     CDS             complement(6627..7919)
                     /locus_tag="IVK93_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6387344.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLAHQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNAAALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLMGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            8069..8629
                     /locus_tag="IVK93_002063"
     CDS             8069..8629
                     /locus_tag="IVK93_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6387345.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELCSMIHGYLSLRFLG
                     YFTKESTIDPEDSYFWMINDFIATLP"
     gene            8758..9135
                     /locus_tag="IVK93_002064"
     CDS             8758..9135
                     /locus_tag="IVK93_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP6387346.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            9068..11140
                     /locus_tag="IVK93_002065"
     CDS             9068..11140
                     /locus_tag="IVK93_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6387347.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKEMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11351..12298)
                     /locus_tag="IVK93_002066"
     CDS             complement(11351..12298)
                     /locus_tag="IVK93_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6387348.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDVDSVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12543..13007
                     /locus_tag="IVK93_002067"
     CDS             12543..13007
                     /locus_tag="IVK93_002067"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP6387349.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(13069..14112)
                     /locus_tag="IVK93_002068"
     CDS             complement(13069..14112)
                     /locus_tag="IVK93_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP6387350.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         4614 a         2596 c         2996 g         4027 t
ORIGIN      
        1 actaactttg taaggacaac gcaaattagt tgtctttttt atttttaaat ttaacacatg
       61 ttgttatttt taatattttg ctgatttatt gtttctaagt aaagaaattt aaattgaaac
      121 gattgttatt tagggaaaaa ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt
      181 gcaaacgcta tacaaaatga aaaagaaaag agggatgttt atggaaaatg aacagtcggt
      241 cgttttaacg aattggaaga gaaattattt gttcttttta tcgggacagt ttttgtcagg
      301 aatcactagt atggtcgtcc aatatgcgat catctggtat ttgacaagag aaaccggttc
      361 ggcgacaatt ttgagttttg ctactttact aggaatgatt cccatggttt tactaagccc
      421 atttgtagaa ccgctagtgg atcgctggga taaaaaagct cttttgattg tcacggatat
      481 tattgtagcg attttcgcgc ttattttagc agtagtcgga acgatttcag aatccttccc
      541 gatttggcta gtttttgtgt ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc
      601 aacgatcagt cgattatgcc gacaatcgtt ccatcttctc acataacaag gacgaatgga
      661 cagttaggaa tggtccaatc agcgaatttc atcattgcgc cagctctagg tgcggctttg
      721 ttttcagtcg ttcctgtcaa ttaccttatt ttattagatg tattgggggc agtttttggt
      781 gtcggcttat tgatttttgt gaagatccca aaagtttctc ctgaaatatt ggaagttccg
      841 cttactattt ttaaagatgc aaaatttggg ctgcagcaat tgatggataa caaagggtta
      901 tggtacataa cgatcaatgg tgcatttgtg atgctgttgt tcatgccagc aatcagtctg
      961 tatccattga tgacgctaga ttactttggt ggttccgttg gacaggcggg agcagtggaa
     1021 gtagtctacg cagtcggaat gctcttaggc ggtgcactga tcagttttat aggaacatgg
     1081 aaagaccgaa tgaaacctat tattatagcc tacatcatta tgggcctgac gatcggtgca
     1141 agcggattgg gtccaaacga tagtcaaggg ttcttgtact ttcttatctt gaatgcagga
     1201 gcaggttgtg caacgccata tttcaataca ctgctgatgg caatgatcca gcagagttat
     1261 gaatcgaatg ttttaggtcg tgtcttaggg aacttcaact ctttgatgaa cctggcaggt
     1321 ccaattggat tattatttgc aggaccatta gctgatcgtt taggggtaga aaaaatgttc
     1381 ttgttttctg gaatcgggat tttattatgc ggaatcgttt tatttttaac gtcagctgct
     1441 cgaaattatg ataaagaatt acagaaaaaa ttggtaaaag aacaccatga gtaaaaagac
     1501 gaatagtatg gaatggagtt gtgacagaag tcttgttaca actccatttc tgatcctaat
     1561 gttcctgtgg caataagcct actcaaatga aaaatatgag taattatctt tatttgttta
     1621 atacttgata tagggcagtt ttttcacgac ctatcgtact tcctctttgc ttcatagatt
     1681 tctttgattt gttgctcaga taacggagtg ccaaatggta gtttttcctt taagagttcc
     1741 ctttccggtg cgtctacgcc tacattgaca tcgttctcga taggtacgct gtaatgatgg
     1801 atatgtccat gaagattgcg gatagtgtcg ttaggcccga gcaacatcgg ataatgagtc
     1861 aagtaatatt gttgatggtc gaatttaacg atggcaccta catcataaaa ataaaatttg
     1921 ggacttccgt cagtaagcct aggatcattt ttttctaaat aacggaaaaa agcgcgactg
     1981 tcatggttcc ctttgatcaa gtggattgtt ccttgcagtt gagtgaccag ttccaaaatt
     2041 tctgcatttg ctttttcata ttgcggatgc atagcaaggt cgcctagatg ataaacatgg
     2101 tcggtttctt ttattactgc attccagcta gtgatcaact gttggttcat ttcatccaca
     2161 tttgcaaaga gccgcggagc aaagtcgttt tttcctaata agcgttcatg gaaaaagtgc
     2221 atatcactgg taaagtaatt catgtatctt ttgcctccct tttttcttag agtaaacaaa
     2281 atcacttatc tgagcaactc ataacatcta aaaagaaaag gttgtaaatg agacgtgtag
     2341 ctttaagcga taagaagcaa ttttgaaaaa tagtttttca caatttataa aattattcct
     2401 tattgccgaa aggctggccc tttttatgag tatgccaatt ttttatatcg aatgctcaaa
     2461 agatgagtga ttttttggtt gactatcatg tttgtgtact gaataggtcc agtagaacag
     2521 tatttttacg acttgataat taagggatga aattttcaga aatatgacgt ataatgtaaa
     2581 atactgaaaa gttgcgacct tcaactcgta gatgtttcag aatctaattg aaatgatggt
     2641 gatatcatga cagacaacag caaaacacgc gttgtcgtag ggatgagcgg cggtgtcgac
     2701 tcatctgtaa cagcactttt gttaaaggaa caaggatacg acgtgatcgg aatattcatg
     2761 aaaaattggg atgacacgga cgaaaacggt gtatgcacgg ccacggaaga ttataaggat
     2821 gtagcgaaag tagctgctca aatcgggatt ccttattatt ctgtgaattt tgaaaaagaa
     2881 tattgggatc gtgtatttga atatttccta gccgaatacc gcgcaggacg tacacctaac
     2941 ccagacgtga tgtgcaacaa agaaatcaaa ttcaaagcat tcttggatta tgcaatggat
     3001 cttggcgccg attatgtagc tactggacat tatgcgcaag tcactcgcga tgaaaacggc
     3061 gtggtccaca tgcttcgtgg agtagacaat aataaagatc aaacttactt ccttagccaa
     3121 ttatcacaag aacagctttc aaaaacgatg ttccctctag gcggcatgga aaaatcagaa
     3181 gtacgggcaa ttgctgaacg cgcaggcctt gcgactgcta agaaaaaaga ttcaacggga
     3241 atctgtttta ttggtgaaaa gaacttcaaa caatttttga gcaactatct gccagcaaaa
     3301 aaaggaaata tggtcactct tgatggtgaa gtcaaagggc aacatgctgg cttgatgtat
     3361 tatacgattg gtcaacgtca aggactaggg atcggtggcg gaggagattc gcaagaaccc
     3421 tggtttgttg taggaaaaga tctagcgaca aacacattat atgtggggca agggttccat
     3481 catcctgcat tgtatgccac aagcttggat gcaagtgaaa tccactttac gacgaatgaa
     3541 ccaatgccaa aagaattcaa gtgtacagcg aaattccgtt atcgtcagca agacgttcct
     3601 gtgacagttc gcttattgga tgataatcat gcagaagtcg tattcgacga acctgtacgt
     3661 gcaatcactc ctggacaagc agttgtattt tatgatgaaa tggaatgtct gggtggtggt
     3721 ttgatcgacc acgcatatca agaaacaaaa gttttacaat acgtttaagc gatattaaat
     3781 gaatcaaaac agctatggct aacaaaagaa acaccttttc ttttggatag catagctgtt
     3841 tttttgcaat aaaattaatt tttattcatt ttatacttga aatacttgtg taaaaagttc
     3901 ataatatcca ataatatgaa taaagttgct ttttgttaag caagagcagt tgcaaaatag
     3961 aaggaaatct tttgcaagtg atttgttcaa ttttataagt gagggtgatg gaaatggaaa
     4021 agaaatcaac gggtattagc aaatggggac tggttgctct ggttgtcagt tcgtcaatcg
     4081 gaagcggtgt gtttgggatc acaagtgatc tggctagttc tgcagctcca gggccagcga
     4141 tcctttcatg gataatcgtt gggattggta ttttagcttt ggtattatcg ctgaatcatt
     4201 taggagaaaa acgtccagat ctagatggtg gtattttcgg ttacgcggaa gcttcatttg
     4261 gtaaattagg tggcttcatc agtggttggg gttactggtt gtctgcgtgg ctgggaaatg
     4321 ttgcttttgc aacgatgctg atgagcgcca tgggagaatt tttcgcgata ttcaaaggtg
     4381 gacaaaatgt tccttctatt ttatttgcaa gtatttttat ttggggtctg actttattag
     4441 taaacaatgg agttgaagga gcgagcgtca tcaatacaat cgtgacgatc tgtaaattag
     4501 tgccattatt cctattttta atatttgtat ttatcgcatt taaagtgaat atattcactg
     4561 ctgatttttg gggtaacgta tctgataatc tagtgaatgg aacagggcag caaggaacga
     4621 tctggttgca gattaaaggt tgtttgatgg ttatgatgtg ggtctttgta gggatcgaag
     4681 gcgcaagtgt tttagccaac cgcgcgaaga aacgttcaga agcccagcaa gcatcgatca
     4741 ttggtctgct ttgtttattg tttatctata tccttgcttc gatcttgcca tacggcgtct
     4801 tatctcaaga agagttagca actatttcac aacctgctat ggcaaatatt ttaaaaggaa
     4861 tcgtgggaaa ttggggagca gcgctgatca atatcggctt gattatttcg atcattggtt
     4921 cttggttgtc ttggacgatg ctcccagcag aaacaacgat gttgatggct cgagacggtc
     4981 tgttgccgaa aggttgggga aaaacgaata aaaaaaatgc cccaacttat tcgttagttg
     5041 ttactgcaat cttgactaat ctattcttat tgactttctt agttactgat tatgcctatc
     5101 aatttgccta ttctttatgt acagcggcaa ttttgatttg ttatctactt gtcgggatct
     5161 atcaagtcca gttttcttta aaagaaggaa tcaaaaaaca agtagtgatc ggtgggattg
     5221 ctgtagcttt tgaattgatg ggcatcattt tagcaggatt ttcttatgtc cttttatgta
     5281 gtattgccta tcttcctggt ttttgcttct actggatcgc ttgtcgtgaa accaaacatc
     5341 aaattacggg aaaagaaaaa gtaatgattg ggctgatttg tgtggccgca ctgctttcaa
     5401 tcgggttgct tgctgtaggc tggatcaaga tataaggggg aaagaaacat gaaaatcgca
     5461 aatttcggtg tagaagaatg gctaaataaa tgggaaaaga aagcggtata tgatattgca
     5521 caaagctcga tcgaagcatt gacattagac gaactcgttg gcttagatgg gactagcgta
     5581 agtgaatttt tcgaaaaacg caaaaacgaa ccattggact atggctggat cgaaggatca
     5641 gatgttttca aacaagaagt cgcttctctt tatcaaaccg tcgatccgga aaacatctta
     5701 caaacgaatg gagcgactgg tgctaatctt ttagctctat acgcgttagt ggaagcagga
     5761 gaccatgttg tttcgatgct tcctacttat cagcaattat atgacatccc caaatcttta
     5821 ggtgccgctg ttgactttgt tcatttgaag gaagaagaag attggcagtt tgatttagaa
     5881 cagctagaag ccttagtcaa acctgaaaca aaaatcatct gtttgaatag tgcaaacaat
     5941 cctacaggca cattgctaga tcggaaaact ttagaaaaaa tcgcagaaat tgctcgtaca
     6001 gtagatgcat atgtattgat agatgaagta tatgcgcctt taacggataa aggtgaattc
     6061 ttgtcgatcg tagatgtcta tgaaaaaggt atagcaacca attccctatc caaaacctac
     6121 tccattccag gtattcgtat tggctggaca gctactggac ctgagttggc agaagttttt
     6181 cgaaagtatc gagactacac gatgatttgc ggcggagtcc tgtcagatga tcttgctgta
     6241 catgcattga agaataaaga aaaaatattg gagcgaaaca aaaagatcat tacagagaac
     6301 cttgtgattt taaaacaatg ggtagccaat gaaccgaaag ttgaacttgt cgctcccaat
     6361 tacgtctcta cttctttcat taaattagcc atcgaagaag atgaccagac attttgtatc
     6421 aatttattag aagaaacagg agtactatta gtaccaggaa gtgcctttga tttaccgaag
     6481 catgcccgac taggttactg ttgtaaaaaa gaaaccttgg aaaagggatt attcctctta
     6541 tctgagttct tgaaaaaaca ggcctaaaca aaaacactgc aaggacagtc tgtgacaact
     6601 gttcaagcag tgtttttcat ttacgcttat tgtctatgag aaggagagtg ccctgaagat
     6661 atggagactc ctgaagcttt ttcttctgca gtttcttgct gatggatctc ttgtgtattg
     6721 tgtcgtacaa gcaagaagtt gaccaaaaca agaagagaag tcgaaagaaa gacgccacga
     6781 taaccaaatg cactagaaac actggagccg atcataggtc ctactacgtt accagttgct
     6841 tggaaagatt ggttataact gaagatccgt ccagcagctt gatgaggaga atattttgtg
     6901 atcaatgctt ggacagcagg gagcagacaa gcatcagaga tacccatcaa aaaacgtaag
     6961 gcagctaatt gccaaacatt cgtgacgaat gccattggga tataaaccag aatcgcaaaa
     7021 cccaaaccaa tcgcaagtat tctttcactc ccaatcttgt cacctaagcg accaaatctt
     7081 ggagcggcta tcaaagtggc aatcccaggt atcgaagcga tgattccgct gatcaacgta
     7141 acattcccat gtccatgcag caattggcga atgtacagac tgatgattgg actaatggaa
     7201 ttgttggatg cttggatgat catcgttgtg acgaacatac cgatgacgac gtgtggatat
     7261 ttcaagtctt tgaaaatctg tttggcagaa accatgtttt cttttttcac tgggacaaat
     7321 ttttcatgga cgaaaaacag actcaacaag aagacgaaaa ataagatgat ccctgtaatg
     7381 aaaaaggtag ggcgatagcc aaacgctgaa gcagagatcc cgccaagaag cggtccaagt
     7441 aatgttcctg taacagaacc agttgctaaa gttccaagaa cttgaccact tttttctttt
     7501 ggcgtcccag ttgcgacaag ggcagcggca ttactgatat aaccagaaaa aatcccttga
     7561 agcagacgca atgcgactaa ttgatacaca ctagtcacgc aacccatcaa agaaatgacg
     7621 attgccattc ctaatgaagc acgcaacagc atcaattttc ttcctttttg gtgggctaat
     7681 tttccccacc aaggagagat cagcgtagtc accaagaaag tagatgaaaa agtcaatccg
     7741 ctccaaaagt tcaattgtga cgtactataa ttacctaacg tatctatgta aagtgacata
     7801 aaaggcatga ctaaactaaa tccgatacca gccataaatg ttccaaacca taagacaaac
     7861 aagttttgtt cccaagtttc tcttttccta aataattttt cctttattct tgcagccaat
     7921 tagactcctt ctttctgaaa attttcatct acagcttttt gttataaact acaatatgta
     7981 gtttatcaat gaaagtctct tttttctctt tctttttcgt ggtaaactga aagagtgaag
     8041 gatttatttt atgagaggaa acatgaaaat gaaaaaaaga aatctgtctc aagagaaaat
     8101 catcgactgc tttcgagaac tagcagaaga gatggatgtt cagcaaataa cctttcagca
     8161 tttagcaaaa cgcttggata tcaaggctcc ctcgctgtat aaccatttca aaaatattcg
     8221 tgatgtcaaa acagcgctaa cagcgaaact tctgcaggaa cttaatgacc agctgcgtcg
     8281 ggctcttgtg ggaaaaagtg gtcaggaagc actgcagatc tatgcagaaa cttatcgatc
     8341 ctttgctttt tcaaatcaag cagtgtatga gttactgatc agtgtgcccc acaccaatga
     8401 agcagtttta ttagagggaa tccatgaaac gaaccagatc attttgcaga tctttgaggc
     8461 ttttcaatta acgaaagaag aaaaagtcca ccgaagcaga gaattatgta gtatgatcca
     8521 cggctatctt tccttgcgtt ttttaggata ctttacaaaa gaatcgacaa tcgatccaga
     8581 ggacagctat ttttggatga tcaatgactt tatcgctact ttgccttaaa caatcagatt
     8641 catttatatc attttcaaga tgttcattaa atgatccttt tataagagaa aattcttgca
     8701 actttcttga aaagtgacta agataagtct agaaaagctg aaagaaggag cacgagtatg
     8761 tatcaagtaa ttaccatgtt cggggacaat gagccttggt ggttttttga agactgggaa
     8821 gaagatattg aagcagaaga aacattcgag tctttcgaag aagcacgaca agcgtacgaa
     8881 aaacaatgga acgaaatcca tcaaaattat gaatatatca atgcaaaaag taactttctc
     8941 agcgcctttt ggaatgacgg agatgagcgc tggtgtgaag agtgtgacga tgatttacag
     9001 caatacaaag gacttgcgtt gttgaaaaat catcagccca tcacagtaga aagcggaaag
     9061 gaattttatg aaacaactaa ttctagcgga aaaaccaagc gttgcgaaag acctaagcaa
     9121 ggtgcttggt gctaatcaaa agcacaaaaa ttattatgaa ggacccaagg tgatcgttac
     9181 ttgggccctt ggtcatttat taggattgaa aatgccggaa gatctgaata aggaatggca
     9241 gagctggcaa atggacacgt tgccaatgat ccctaaaaaa ctaggaatca aaccacttcc
     9301 taaaacagga catcaattaa aagcaatcaa gcagctggct cagcgaaaag atgtctcaga
     9361 agcagtcatt gcgacagatg ccggaagaga aggggaactt gttgctcgct ggatcttaga
     9421 gtatgtgcat ttcaataaac cagtgaagcg gttatggatc tcttctcaaa cagataaagc
     9481 aatcaaaact ggattcaagc aactaagacc tgcgaaagaa tatgatgcat tgtatgaatc
     9541 tgctcttgcc cgggcaaaag cggactggct ggtaggattg aatgtgacca gagccttaac
     9601 tgtgaagtac gcggataatt tatcagcagg ccgtgtgcag acaccgacac tagcattagt
     9661 gagagaccaa gaaaagaaaa tcgagcagtt ccgtccacaa acgtatttca cgatcacgtt
     9721 gcaagctgga aaagaacaag ccaaaatgct tcaaaaaaat caatttgctt taaaaacaca
     9781 agaagaagca caaaaacttg ttcaattgat cagtacaagc aaaggaactg tcacagaagt
     9841 ccaagaaaaa ttaaaaacgg aatctgcgcc attgccttat gatttaacag agatccaaag
     9901 agaagccaat caacgctatg gctattctgc aaagaaaaca ttaagtcttg tacaaagttt
     9961 gtatgagacc cataagatcg ttacttatcc gcgtacagac agcaaatatc tgacaaatga
    10021 catgaaaagt acgatgaaag aacgattgca agcagtcagc gactttgctc ctgaggtcaa
    10081 gcagtatttg aaaaacggag ccatcgtcca acagacaaaa gtattccaag acagtaaagt
    10141 gaccgatcac catgcacttt tgccaactga aaacagagca cgttatgaaa aattaagtaa
    10201 tgaagagcaa aaaatctatc aaatgattgt ctctcgtttt ttaggactgt ttgctcaacc
    10261 ccataaagtc agccaaacaa aagttacggt tgaatttggt aaagaacgat tcatttttcg
    10321 gcaaaatcgt gtcgtccaag caggatggaa aggcgaaact gaaactgaga cggaaacagt
    10381 caaatgggaa aaagaaatga gaatcaatcc cgatttcacc atcaaaaaag aactatctgc
    10441 accgcctaaa ccattgtcag aagcaagctt gcttggtcaa atggaaaaat acagtctggg
    10501 aaccccagct acgcgtgcag agattatcga gaaactaatc aaatctgaac tgatggaacg
    10561 tacccctcaa ggactgcaag tatctcctaa aggtaagcaa ttattagtat tggtaaatcc
    10621 ctctttagta acaccagatt tgacagaaag ctgggaaaaa gaccttgaag ctattgctgc
    10681 aggaaagaaa caagcaggaa ccttcttgaa tggaatcgaa aaagaaacaa aacgcttggt
    10741 gaatgagatc aaatcaagca aacaagaata tcaagatttc tcgattaccc aaaagaaatg
    10801 cccagaatgt ggggccaatc tgcgcgagaa aaacacacgt gacggcaaaa tctatgtctg
    10861 caccaattca gattgcaact accgcagaag aaaagatcca aaagtctcta atcatcgatg
    10921 cccgcaatgt caccggaaaa tggaaatcat cgaaggtaaa aacggtgctt atttccgttg
    10981 taaatacgat ggaacgacag aaaaaatgct tgataaaaaa gagcagaaga agaaaatgac
    11041 caaacatgaa gaacgacgct tgctgaaaaa atatgcacaa gcagaagaac cagaagaaag
    11101 cccattagca gcagcactaa aagcagcaat gaagaagtaa taagatatga aagaggttgt
    11161 gatagaagtg gtcagcttca agaaataaga aggaatttac gaaaattgct tctcaaattt
    11221 ttgtgaaatt tcggcttatt tccgaagaag ctacttctgg aacaccattt attcggaaaa
    11281 aaggactgtg acaagacttt tgtcacagtc ctttttcatt taaaaaaatg tttacttcat
    11341 ttgacgtata ttattggtga ggcttcaatt catttggctt cactttttct gtttctctta
    11401 tgatataaac aggacgctgt ttcgtttcta agaagatttt cccgatatat ttcccgatga
    11461 ttccaagaca taacaattgg attccaccaa tgaacaggaa gatcgagacc atggaaggcc
    11521 atcctgatgt aggatcacca aacaacaatg cccgcaaaat aatgaacaaa attgcaatac
    11581 cagaaccgac acaagacaaa gcgccgataa atgaagcaat cgttaacggt gcatccgaga
    11641 agttcacgat accatcaatc gagtagttga ataatttcca gaaggaccaa gatgtttcgc
    11701 cagccacacg ttcgcggttt tcataagaaa tatattctgt tttaaatccg acccagctaa
    11761 ataatccttt tgaaaatcgg ttgtattctg ttaattctag gacggcatcc accatttgac
    11821 gagtcatcaa gcggaaatca cgagcgccat cgaccatttc tgtatcgctg attttattga
    11881 tcaactggta gaattttttc gcaaagaaac tgcgtatcgc aggctcgcct tcacgagtcg
    11941 aacgccgggt gccgacacta tcgacatcag aagtagtgat ccggttgatc atctcaggaa
    12001 gcagttcggg cggatcttgc agatcaacat ccatcacggt cactaaatct cctgtagaag
    12061 cttgcaatcc agcgtacaga gccgcttctt tcccaaaatt tctggaaaat gagacaaagc
    12121 ggacataagg ataagctgag gctagcttct tgattgctgc taacgtaccg tcttttgatc
    12181 cgtcgtcaat aaacaaatat tcgaactcat gggcaccttt gaatttttca acctcatcaa
    12241 aaaaaagagg aacagttttt tcttcattaa aacatgggac aacaattgat actttcatcg
    12301 tttctatcct ttcaatcgta tgtttatata tggtttacat tcaaaattta ataaacctta
    12361 aataacacct tttaaatttg attttatgga aaagaacagc tattctttgg taaactaacg
    12421 tatttagttt accatgtttc ctcggtttat caaactttta agaacaattt ttattagttg
    12481 gcaagtagtt ttggatagag aaaaaggagg aagttccagt ctactgacca ggagcaaaca
    12541 aaatgaaaat acacacacct atcggctttt tatggttaga tgcagatgaa tccggattga
    12601 ctgctgtatc ttttcagcca atttcagaaa gcgaatcagc gaatcagcaa attttaacgc
    12661 gcacacaaga acagtttgaa gaatattttg cgggaaaaag aaaagaattt gatgttcctt
    12721 tatctataaa gagaggtacg ccttttcagc aaaatgtctg gaaagctttg tcgaccattc
    12781 cttatggaga aatccgaacg tataaagaag tagctgttgc tgttgatagt ccgaaagctg
    12841 tgcgagcgat tgggcaggca aatcgaacca atccattgcc aatcgttatt ccttgtcatc
    12901 gagtgatcgg gcaaaatgga aaactgacag gatatatggg aaacgcaata gatggggttg
    12961 aaatcaaacg ccaattgttg ttgttagaag gatatctgca aaattaaaaa cagttgtgat
    13021 tcggacattg ttccatgcga gaatcacaac tgttttttca tgttcattct aaactttgat
    13081 cagccaaata aatagtaaag ccatcactgt ttggagaagc ccgacagcta atccataaga
    13141 taagaagcgt gtaccttctt tcatgaattt tgcaaagtct aaacgcagac caattgccgc
    13201 taaagcagtg atctcaaacc agctgctgat aaaatgtgcc gattggtcaa taactacagg
    13261 taaagaaaac aagctgttga ccaaacaaaa gatcacaaac cctaatacat accaaggaag
    13321 tggtaatttt cgttttttct gcgtatcagt tggcgtttca aacagagctg gttgtttctt
    13381 gtgcatgaat ttttcaaaca gatagacaac aacaacgagc atcataatac gtagaatctt
    13441 aaatagcatg gcgaattcga caacagaatt gtcaagtaag cttgccccag cgactacttg
    13501 accaacagat tggagtgtgc cacccaaaag tgcacttttt tcaatgagat ttccaccaaa
    13561 caactgtaat ccaaggaaag ggagagtcaa catcatcaca gtacccagta gattgactag
    13621 tgtgatgatc tgtccttttt cttcctcttt cgcatggatg gaaggggcga tcgaagcaat
    13681 agcagaagaa ccacaaacag cgtttcctcc agccatcatc aaagaagaag tatcgctgaa
    13741 ttgcagtttt ttaccaatca agtaagcacc gatgatagtg actgacattt gcagtaacac
    13801 aaaaacaatt ccttttatac caatttgagc aatcgtttga gctgtgacag ttgcacctag
    13861 taaaaaaaca gagaattcta atagtttgct ttcagcaaat tttgtgccga ttgctagaac
    13921 aggctgttta aaaaaagtat ttcctaataa aatacccaat aaaatagcaa tagtcgctcc
    13981 acctaactgt ggcagataaa tagcaagaag cctgctgaag gcagcgatgg caaaagaaag
    14041 cagtaaaccg ggtaagattt cgcggaccgc tgaatccttt acagatttta ttggatgtga
    14101 caatgatttc atttgatcgg ttcctttcgt caataaatac taaaagcata ctcgaacata
    14161 gtagcactaa accagtattt tgacaaaaga acaagaggtc gtgacaaaag tcttgtcacg
    14221 acctcttgtt tcg
//
LOCUS       DACCCR010000068        14229 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCCR010000068 DACCCR010000000
VERSION     DACCCR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.075"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            419..631
                     /locus_tag="IVK93_002069"
     CDS             419..631
                     /locus_tag="IVK93_002069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387351.1"
                     /translation="MDALRKLEVTLYNKQWFTLIGIEACLKNQDGFSAEEVLDQIQGC
                     LEEYKHVEGAMMEEYQQQNFAKLETV"
     gene            729..956
                     /locus_tag="IVK93_002070"
     CDS             729..956
                     /locus_tag="IVK93_002070"
                     /inference="COORDINATES: protein motif:HMM:NF037117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387352.1"
                     /translation="MRKTISLTSNNIKVSGHIGTWYVYASRVYHGRRLFLVEHETYGD
                     HAANLILDKTGNCVMEDVWNGWEDYEVYIES"
     gene            1110..1562
                     /locus_tag="IVK93_002071"
     CDS             1110..1562
                     /locus_tag="IVK93_002071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387353.1"
                     /translation="MICRYCNKKVLKLEGGQYHCANCELDFFENQLIEFRSGLYREPT
                     VETDPFFFQYTTTKELLQMTYVELLSRLKLARGVYRELRKKVYGHNSQADDLDRENLK
                     AVTSAKLVIENIMIEKHGYYPASVFESVIDKEVLKNKKQMREVEKKHG"
     gene            1555..1806
                     /locus_tag="IVK93_002072"
     CDS             1555..1806
                     /locus_tag="IVK93_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387354.1"
                     /translation="MVKFEDQPKQGYTGIRTLETIASVFEDIQASGDMGQGEKNLRYL
                     QLMNELEEDHGAMIVNPTKTDLNLPAVALYRKVSQARVL"
     gene            1831..2139
                     /locus_tag="IVK93_002073"
     CDS             1831..2139
                     /locus_tag="IVK93_002073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387355.1"
                     /translation="MDKSKEIIFMPDYLVKDHTVLVLDSANNLHGFLSIESTDDHFVF
                     TNDIDLLKTVPKIIAEELATDIRASEGDYIPVTNKDTGEVSELGSDDLQIVFSAVEWC
                     "
     gene            2213..2677
                     /locus_tag="IVK93_002074"
     CDS             2213..2677
                     /locus_tag="IVK93_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387356.1"
                     /translation="MNEPDMTNQFRVGEVILQRMIDRKEFEDIYRSVTRKNTVDGYYF
                     DAGRRIVTVFAYYLGSISGSSVDEERIIGELMALMATMERDSIPGVVVWDKISKLPGT
                     PEHSMQLASEKAIEDTFTFLLAAAKAEEPYYLNGGTETIVSMIAAEVEQDCR"
     gene            2696..3124
                     /locus_tag="IVK93_002075"
     CDS             2696..3124
                     /locus_tag="IVK93_002075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387357.1"
                     /translation="MLKCYGEAELLEMLKRNEFEEDSPIRSVEDLLGHLDQASPMMWV
                     TVYVPEVQSVYGIHRETLISTGQSHRSTSKPVALLEATGYQTSEHVFVSELAALLQME
                     VVPKNREIFELQLFERKRGKYMLRRWQMIGDQPVLIAQRG"
     gene            complement(3294..4160)
                     /locus_tag="IVK93_002076"
     CDS             complement(3294..4160)
                     /locus_tag="IVK93_002076"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HAP6387358.1"
                     /translation="MITLKNHKLELLQFTDIHIGQAPFNEEDQKTFAMIDQTLAKTTA
                     DLIVITGDLIWSDGVIEPTKGLEALAEIFNKYPIPLAITYGNHDSEETIDRHDLHELE
                     KKLFNHLALKANQFFDPNQKECFTIEIKDDNQLVNVLYFIDSGANALIDYESYDWVSL
                     EQIKWYDETFAKYQKINHTKDLLFLHIPLPEYLQAGERIVEGRFWEMNPRISAPKLNT
                     GLFSHLLENNHLLGTFCGHDHDNNFEGIYLGQRLIYGNVTGYNCYGDLPRGYRKITLK
                     PDQMTTSIETFE"
     gene            4341..5288
                     /locus_tag="IVK93_002077"
     CDS             4341..5288
                     /locus_tag="IVK93_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007474874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6387359.1"
                     /translation="MKQDSYSVKDANVILSKLILGSSDYLKLDNKDKVDRMFTRYVEE
                     GGRTFDTARHYRESEAVIGSWLKDKKREDYTIITKGCHPVREAPDTPRVNPESILADL
                     TTSLEMLATDYVDVLLLHRDDPSQPVGPLMETLSQLVDEKKIRSFGVSNWELPRIIEA
                     KKYCEDNGLNPLSFNSPNFSLAKVNEPRWENCVTADDLMIAWHEETDFPLISWSAQAE
                     AFFGNRFSPDDVNDPEMAEFVAVYFNDLNWKRLVICEELAKEKGVKPIQISLAYVLSQ
                     TFPSYATIGPEEEWQLLDSVEAMNIQLSAEEIMRLKKGE"
     gene            5321..6058
                     /locus_tag="IVK93_002078"
     CDS             5321..6058
                     /locus_tag="IVK93_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6387360.1"
                     /translation="MYSVRKELEKDPENTFKSLKEIGFEAVQLDGMRGHKETEIKALV
                     DKYGFKIAGMHIKHDRFFNDRDGIISESLLFGCKTIYDKYIDDEEQHTKGYIQTKQQL
                     VTTQATLGKLGFRIGLHCPEYDFNQTVEGRTILDYITDPVFGTAVYAEPDTYWISVAG
                     KDPLEEFRKFSGRAPIVHLKDYIKGFAPEDMDNNLTELGNGDVDFKAIVEWGEKSGVE
                     YYCIEQDYSKIGIFNSLAKSFDHLMSI"
     gene            6075..7328
                     /locus_tag="IVK93_002079"
     CDS             6075..7328
                     /locus_tag="IVK93_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6387361.1"
                     /translation="MKKNSRIVMMGVALVSGLVLAGCGNGSASNSDKVELSINYYNGA
                     ISKKTMDNAKKEFSDYKLSFKQIPANDDFDTKLKASLNSKSAPDITAINSNISDYLPY
                     ADKFVNLLDYDSETLAADFVEWKWDSCFTNDKKTQIAMPIDIGPTALFYNVDAFAKAG
                     LPTNDQEISKALKDEESLMAAAKQMKEKADMPFFQSSLSLLQSKNRTMTEHIYDENGK
                     LTYANGQLKEIWDYAVKAEKSGYTLGTKGNSADGANSVQKGMFGGMIEASWGINDLAE
                     NGVSPKQWMIAKSPFTASNYGGSYLAVIKTSSHPKEAAEVVKYLTNEDSQRANYEELA
                     LFPSNTKVYDDSFKAAKNELFGDEEFNHYFIDAANELEYVPSDPRESAAFKCFEDQII
                     LVAEQGKDPEKAWNDAVKKVEEMSK"
     gene            7429..8334
                     /locus_tag="IVK93_002080"
     CDS             7429..8334
                     /locus_tag="IVK93_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6387362.1"
                     /translation="MNSESKSKKKFSFDKVAPYVFVSPFYILFLIFGLFPVIFSGVLA
                     FSKWDGLGDIQFVGLDNFTRLLRDADFYLSVKNTLIIWAVGTFPMLFLALILAFLVNL
                     KFIKHKELLKSIFFLPNITSVVAVTILFGLIFSNLDGLANALLQFLGFEPIKWVTSPF
                     WVRIVIALINIWMYLGYNMIIYLTGITKIPNDYYEAAVIDGATNMQIFWNITIPQLKP
                     IILFTVMMSTIGGLQVFSEAQVLVPQGATPEGGAMTIVYYLYNTAFTNNEYGYGSAVA
                     WALVLIILVFSLINWYLTTYRIKGE"
     gene            8337..9161
                     /locus_tag="IVK93_002081"
     CDS             8337..9161
                     /locus_tag="IVK93_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6387363.1"
                     /translation="MSNRIFKILVRAFLAIGVFLSIFPFYWMIVMASKDNSEIYHIPP
                     DLMLGSELMNNIKTVFTETTFFSSLLNTLFVALTSTLLILFFDSLAGFSFAKLNFKGK
                     AFLFTFLLGTMMIPGQLNIIPSFYLMDKFGWVGSYKALIIPGMANAFGIFWIKQYCTD
                     AIPDSLLESAKLDGCTLFKAYYKVVLPIMKPALAFLALYSFMGAWNDYMWPLIILNDE
                     SKYTLMLALTQLQGLYKTNYPLVITGTLLATLPLLLVFTIFSKQLISGITEGAVKQ"
     gene            complement(9274..9561)
                     /locus_tag="IVK93_002082"
     CDS             complement(9274..9561)
                     /locus_tag="IVK93_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6387364.1"
                     /translation="MKEKNPYYRLKYVRIYKRRGKKRAIITIARMMLTAIYHMLSTGE
                     VWNPTDLYKIDMPDNLVQKQKDKAIKQATKLLISEGLLPDDFVRKDLEPAI"
     gene            9897..10487
                     /locus_tag="IVK93_002083"
     CDS             9897..10487
                     /locus_tag="IVK93_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387365.1"
                     /translation="MEVNMKEKFIALQAKLMDLILLSGLILIGAMTIIGFFYGIVAAF
                     VYLETKSEDKWQVLKKRQTILISLLLEMSAVFVAGIFFLNARLLSQLQGGATIIISFF
                     LAVCTLILIPLYFILLHKLAVGTIFTKETFLNSVKVILIGFPTWFTQIILMIALSVLT
                     YLFPALSIITVGYLITRTNKIINVQKMKLASLQEVY"
     gene            10487..12496
                     /locus_tag="IVK93_002084"
     CDS             10487..12496
                     /locus_tag="IVK93_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6387366.1"
                     /translation="MKLSQREIDLLKLALDGEEETTVPQLKKQLKLSVHQIRYAIDKV
                     NRFLIEKKLNQIFIEKDRLVIEQRRKIKKEIDFFISHSTPEQFKFTAEQIEYFISLKL
                     LLSDQRLPINYFVETLSTSRTSVVNVLDRITQELAEEEIELSNVPRKGYMIEEYSFKR
                     FSYFITILKKMINIREIYSFYYQDNLYSKAGDLVFFDLFDLDILFDSLGETIQYSKQM
                     ASEIDDTNFLFLTILNYKYRELINAFEMTDRTEKLQMISSFISEIKQNNPNQMENNNL
                     AKRLIRYINRELADVFEVKDFKVASNALLYHIQRFIFRQENGIPFRDIDSEVLVKDHK
                     NLYYVIFAAIRSFQNGLFESVEAPEAVLITLYYINEIEKNAPKVFKAPRILIICGEGR
                     AISSVLKIKISNLIDSKWIDTISVFEFETPLIDHYDLIITTVRLPEVESDKVLYSDNV
                     FSESFFEQIKLKLSVGGYSFATQRLGKLSQIMNAIVEEVNDDINMNRLEAKIIKILAN
                     NLLQNNRPNEQKFIFDASMLKYHQAAQTWQNAIRISGQELLAKHSIEEQYVNNIISNI
                     ENFGPYMIIAPGVLLAHAGEQDGVLNNGFSMHVFHEPITFPGENVFPVSVIMTIAVKD
                     RTSYHVVERIVQWVLDEETIFSLADEKNRQRIEQMIRNILMMQEV"
     gene            12504..12809
                     /locus_tag="IVK93_002085"
     CDS             12504..12809
                     /locus_tag="IVK93_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6387367.1"
                     /translation="MTTDEISFALISKAGTAFSMMIEALGDAREKDFEKAEEKKQEAE
                     ALMNEAHNLQTNLIVNEVNGDDNQINALLIHAQDTLMNTILMSTIVDEFLNLYKAGE"
     gene            12811..13146
                     /locus_tag="IVK93_002086"
     CDS             12811..13146
                     /locus_tag="IVK93_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6387368.1"
                     /translation="MEKLSICLACNLGSSTSILVNKMKQVAAGSQKLSSFDLMIDAIP
                     AGNISTEQVKDYQIIMLGPQIGHRRNEIEQKVASINIPVTVIDAADYGSMNAANILKE
                     AIYQIKTAN"
     gene            13159..14160
                     /locus_tag="IVK93_002087"
                     /pseudo
     CDS             13159..14160
                     /locus_tag="IVK93_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389903.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
BASE COUNT         4425 a         2373 c         3074 g         4357 t
ORIGIN      
        1 aaaagcaaaa ggattgaaag gtcttgctcg aagcatagct ccgacttctc gcaagacggc
       61 ggggaaatca cccgcctgaa aattccgagc gaattttgtt ttgttcgtca atcgccgctc
      121 ctattccggt ttcattttgt gtgcaaaggt tgtttgctgt ttgtggttat agatcaaacc
      181 cggtggcagg taaagggtat acaagcagca aaatccgccg ccctttacct gccaccgggc
      241 ccgatctaga gcataaccac agcaaacgac ctaagcacac tggaaaaaat gaacccatga
      301 caaataggaa caaagggcac gactggacaa accaaaattg gaatttcttt aaagattcca
      361 gctccgccct agctcctaaa aaaatgggtt tcaaaccaac aaaaaaggag ttttttacat
      421 ggatgcttta agaaaattgg aagtcacact ttacaacaaa caatggttta cactgattgg
      481 gattgaggct tgtttgaaaa atcaggatgg tttttccgct gaagaggttt tggaccaaat
      541 acagggttgt ctggaagagt acaaacatgt tgagggggca atgatggaag aatatcagca
      601 acaaaatttt gctaagttgg aaaccgtgtg ataccagctg aacaatcgga aatgcaaggc
      661 atgtaattcg gatgacgacc tggtccttgc caggttgttt tccggaatca ttgaaagggt
      721 gttgaattat gcgaaaaact atttcattaa catcgaacaa tatcaaggta agcgggcata
      781 taggtacgtg gtatgtatac gccagtcgag tgtatcacgg acgtcgttta tttcttgtag
      841 aacatgaaac ctacggggat cacgctgcca acttgatact ggataaaacg ggaaactgtg
      901 tcatggaaga tgtttggaat gggtgggaag attatgaggt gtatattgaa agctgaaggg
      961 aaagagtagg caccagtctg atcttaggat gacctaagct gagagtcttc agcttgggta
     1021 tgttcttgta ggctaattct tggtaaaaaa gaagagcagc attgtttatt tagcatgata
     1081 tactatttgt gaaaacgggg ggatttgtga tgatttgtcg atattgtaat aaaaaggttt
     1141 tgaagctaga agggggccag tatcactgtg ccaattgtga gctggatttt tttgagaacc
     1201 aactgattga gttcaggtct ggattgtatc gagaaccgac cgtcgaaaca gatccgttct
     1261 ttttccaata cacgacgaca aaagaattat tgcagatgac ctatgtagaa ttgctgtctc
     1321 ggctgaagct ggcgagaggc gtctatcgtg agttgaggaa aaaagtttat ggccacaata
     1381 gccaagcaga tgatctggat cgcgagaacc tgaaggcagt cacttcagca aagcttgtca
     1441 tcgaaaatat tatgattgaa aaacatggct attatcccgc cagcgttttt gagtctgtta
     1501 tagataaaga agttttgaaa aataaaaaac aaatgcgaga ggtggaaaaa aagcatggtt
     1561 aaatttgagg atcaaccgaa gcaggggtat acaggtatcc ggacgctaga aacgattgcg
     1621 agtgtatttg aggatattca ggccagcggt gacatgggac agggcgagaa gaatttgcga
     1681 tacttgcagc tgatgaatga gttggaagag gaccacggag ctatgattgt gaatccgact
     1741 aaaacggatc tgaatttgcc tgcggtcgca ttataccgga aggtttctca agctcgagta
     1801 ttataggcgg aacttgagag gagtttacga atggataaat cgaaagaaat catctttatg
     1861 ccagactatt tagtaaaaga tcatacggtg ctagttttag attcagcaaa caaccttcac
     1921 ggttttttga gtatagaatc aaccgacgat cattttgttt ttacaaatga tatagacctg
     1981 ctcaaaaccg tcccgaaaat tattgcggaa gaacttgcaa ctgatattcg cgcttctgaa
     2041 ggcgactata ttccagtaac aaataaagat actggcgaag tatctgaatt gggttcagac
     2101 gacctccaaa tcgtattttc tgcggtagag tggtgctaaa ggtagcggct gagtctaacg
     2161 atcatgatga atgcatggat tcaactaata ggagaaaaga aggtagaaat taatgaacga
     2221 gccggatatg acgaatcagt ttcgagttgg ggaagtcatt cttcagcgaa tgattgatcg
     2281 gaaagaattc gaagacattt atcgctcagt gacccgaaag aacacggtgg acggctacta
     2341 tttcgatgca gggcgtagaa tcgtaacagt atttgcttat tacttgggaa gtatctctgg
     2401 ttcttcagtc gatgaggaaa gaatcattgg ggagcttatg gctttgatgg caacaatgga
     2461 gcgcgacagt attcctggtg ttgttgtttg ggataaaatt tcaaaacttc caggcacacc
     2521 ggaacattcc atgcagcttg cttcagaaaa agcaatcgaa gacaccttta cttttttact
     2581 tgcagctgca aaggctgaag agccgtatta tctcaatggt gggacggaaa ctatcgtatc
     2641 aatgattgct gctgaagtag aacaggattg taggtagcag caggaggcag aaaatatgtt
     2701 gaaatgctat ggtgaagctg agctactaga aatgcttaag agaaacgagt ttgaagagga
     2761 ttcaccgatt cggagtgttg aagatctgct tggacacctg gatcaggcca gtccaatgat
     2821 gtgggtgacg gtgtatgtac cagaagttca aagtgtgtac ggtattcatc gagagacgtt
     2881 gatttctacg ggacaaagcc ataggagtac atctaagcca gttgctttgt tagaggcgac
     2941 aggttatcaa acaagtgaac atgtctttgt ttctgaatta gcggcgcttt tacagatgga
     3001 ggtagtcccc aagaatcgag aaatctttga attacagctg tttgagcgaa aacgggggaa
     3061 gtacatgttg cgtcgctggc aaatgattgg ggatcagcca gtactaattg cccaacgtgg
     3121 ctaattatta tttaaagatg agtgttgtgt aagctgaggt gacctttatt ggatgaaaag
     3181 caacaaaaac gacaactact aattaaatta gaatcagtcc taaaatgaat gactatttta
     3241 ggactgattt tgctacataa aaaacagcta gcagattact agctgttctt tctttattca
     3301 aaagtttcga tggaagtcgt catttgatcc ggttttaagg tgatcttacg gtagccccgt
     3361 ggcaaatcgc cataacagtt ataaccggtg acatttccgt aaatcaatcg ttggcccaga
     3421 tagatgcctt cgaaattatt atcatgatca tgaccgcaaa aagtgccgag taaatggttg
     3481 ttttctagta aatggctgaa taagccggta ttaagctttg gtgccgaaat tcgcggattc
     3541 atttcccaaa aacgcccctc tacgatccgt tcgccggctt gtagatattc aggtaatgga
     3601 atgtggagga acaatagatc ttttgtatga ttaatctttt gatatttcgc gaaagtttcg
     3661 tcgtaccact tgatttgttc taaagaaacc cagtcgtagc tctcataatc aattaaggca
     3721 ttggcgccgg aatcgataaa ataaagaaca ttaactaatt gattatcatc tttaatttca
     3781 attgtaaaac attctttttg atttggatca aagaattgat tggcttttaa agccagatga
     3841 ttgaagagtt ttttctctaa ttcgtggaga tcgtgacgat caatggtttc ttcggagtcg
     3901 tgattgccgt aagtgattgc caatggaatc gggtatttat tgaaaatttc ggctaaagct
     3961 tctaaccctt ttgtaggttc aataacacca tctgaccaaa ttaagtcgcc ggttatcacg
     4021 atcaaatcag cggttgtctt cgctaaggtt tgatcgatca tggcaaaagt tttttgatct
     4081 tcttcattaa aaggagcttg accgatgtgg atatcggtaa attgcaatag ttcaagtttg
     4141 tgatttttta aagtaatcaa gggtaacatc ctttcttaat acgttattaa aataaaccct
     4201 atcaaaaaat aatgcaaacg ctttaataac tttttttaag attaaaaaaa taaaatatgt
     4261 cattcaagac gttgaaagcg attttagaat ttgctattct ttgggtgtgt tgaagagagc
     4321 aaaaagaaag gggcttcaga atgaaacaag attcttattc agttaaggat gcaaacgtta
     4381 ttttatcaaa attgatcttg ggttccagtg attatttgaa gttggacaac aaggacaagg
     4441 tcgataggat gttcactaga tacgtagaag aaggtggacg aacttttgac actgccagac
     4501 attaccgtga aagtgaggcg gtgattggca gttggttaaa ggataaaaaa cgtgaggatt
     4561 acactattat cactaaaggt tgtcatccag tgcgggaagc accagatacg ccgcgtgtta
     4621 atccagaaag tatcttagca gacttgacta ctagtttaga aatgttggca actgattatg
     4681 tcgatgtgct tttactgcac cgagatgatc ctagccagcc ggtcggtcca ttaatggaaa
     4741 ccttaagcca attggttgat gaaaagaaaa tccgatcttt tggtgtttct aactgggagt
     4801 taccacgaat tattgaggca aaaaaatatt gtgaagataa cggattaaat cctttgtcat
     4861 tcaatagtcc aaattttagt ttagccaaag ttaatgagcc tcgttgggaa aactgtgtga
     4921 cagcagacga tctgatgatt gcttggcatg aagaaactga ttttcctttg atttcttggt
     4981 cggcacaagc agaagcattc tttggaaatc gcttctcacc ggatgatgtt aacgatccgg
     5041 agatggcaga gtttgttgct gtttatttta atgacttgaa ttggaagcgc ttagtgattt
     5101 gtgaagagtt ggcaaaagaa aaaggtgtca agccaatcca aatttctctg gcctacgtgt
     5161 taagtcaaac tttcccctca tatgccacaa ttggtccgga ggaagaatgg cagttacttg
     5221 attcagttga agcgatgaat attcaattat ctgctgaaga aatcatgcgt ctgaagaaag
     5281 gagaataagc cagttggata aaacaaagat tgcggcccaa atgtattccg tccgtaaaga
     5341 actggaaaaa gatccagaaa atacttttaa atcattgaag gaaattggtt ttgaagccgt
     5401 tcagcttgat ggtatgcggg ggcacaaaga aactgaaatc aaagcattag ttgataagta
     5461 tggtttcaaa attgccggga tgcacattaa acatgatcgt tttttcaatg atcgagacgg
     5521 aattatttct gaatcattat tgtttggttg taaaacaatc tacgataaat atatcgatga
     5581 tgaagagcaa catacgaaag gctatatcca aacgaaacaa caattggtca cgactcaagc
     5641 gactcttgga aaactcggtt ttagaatcgg ccttcactgt ccggaatatg attttaatca
     5701 aacagtcgaa gggcggacca ttttggatta tattacagac ccagtttttg ggacggcggt
     5761 ctatgcggag ccagatactt attggatctc ggtagctgga aaagatcccc tagaagaatt
     5821 tagaaaattc tctggtcggg caccgattgt tcatttgaaa gattacatca aaggttttgc
     5881 accggaagat atggataata acttgacgga attaggtaac ggagacgtcg attttaaagc
     5941 cattgttgaa tggggcgaaa aaagtggtgt tgagtactac tgtatcgaac aagattattc
     6001 caaaatcggt atttttaaca gtttagcgaa aagttttgat cacttaatgt caatttaatc
     6061 ttaggagcga gaagatgaag aaaaatagtc gtattgtcat gatgggtgtc gcattggtaa
     6121 gtggtttggt attagcaggt tgtggaaatg gcagtgcctc aaatagtgat aaagtggaat
     6181 tatctatcaa ctattacaat ggtgcgatca gtaaaaaaac catggataat gctaaaaaag
     6241 aattttctga ctacaaattg agttttaaac aaattcctgc caatgatgat tttgatacaa
     6301 agctaaaagc cagcttaaat tcaaaatcgg caccagatat cacagcaatc aacagtaata
     6361 tttctgatta cctgccatat gctgataaat tcgtaaacct attggattac gatagtgaaa
     6421 cattagccgc tgactttgtt gaatggaagt gggattcttg ttttacaaat gacaaaaaaa
     6481 cgcagattgc catgccaatt gatattggcc caacagcttt attctataat gtagacgctt
     6541 tcgcaaaagc tggtttgcca acgaacgatc aagaaatcag taaagcattg aaagatgagg
     6601 aaagcttgat ggctgctgcc aaacaaatga aagaaaaagc cgatatgcct ttcttccaat
     6661 cttctctttc attgttgcaa tctaaaaatc gcacgatgac tgaacatatt tacgatgaaa
     6721 atggcaaatt gacctacgct aacggtcaat taaaagaaat ctgggattac gctgttaaag
     6781 cagaaaaaag cggttacact ttgggaacaa aaggaaatag tgctgatggc gccaactccg
     6841 ttcaaaaagg catgtttggt ggcatgatcg aagcttcttg ggggatcaat gacttagctg
     6901 aaaatggcgt ttcaccaaaa caatggatga tcgcaaaatc tccatttacc gcgtctaact
     6961 acggtggttc atatttggcg gtgatcaaaa cttcaagcca tccaaaagaa gctgcggaag
     7021 ttgtgaaata tttgacgaat gaagacagcc aacgggctaa ctacgaagaa ttggctttat
     7081 tcccatcaaa tactaaagtt tatgatgatt ccttcaaagc agcgaaaaat gaattattcg
     7141 gtgatgaaga atttaatcac tactttatcg atgccgctaa cgaattggaa tatgtaccat
     7201 cagatccacg tgaaagtgct gctttcaaat gtttcgaaga ccaaatcatt ttggtagctg
     7261 aacaaggcaa agatcctgaa aaagcttgga acgatgccgt gaagaaagta gaagagatgt
     7321 ctaaataatc agaatgtgat ttcgggatat gacatcgatc aaattttaaa aattggtggg
     7381 tgtcatagac ccgtaaatgg aggtctaagg atggagattg gtgagatgat gaattcagaa
     7441 tcgaaatcaa aaaagaaatt tagttttgat aaagttgccc cttacgtttt tgtatccccg
     7501 ttttatattt tgtttttgat ctttggtctg tttccagtta ttttctcagg cgtcctggcc
     7561 tttagtaaat gggatggctt aggggatatc caatttgtcg gtttggataa ttttacccgt
     7621 ttattgcggg atgctgactt ttatttatca gtcaaaaata cgctaatcat ctgggcagtt
     7681 gggacattcc cgatgttgtt tttggcacta atattagctt tcttagtaaa tttgaaattt
     7741 atcaaacata aagagttact aaaatcgata tttttcttac caaatatcac ttctgttgta
     7801 gcggtgacaa ttttatttgg cttgattttc tcaaatctgg atggcttagc caatgcgcta
     7861 ttacagtttt taggatttga accaattaaa tgggttacgt caccattttg ggttcgaatc
     7921 gtgattgctt tgatcaatat ttggatgtac ttaggctata acatgatcat ttatctaaca
     7981 gggattacaa aaattccaaa tgattactat gaagctgccg ttattgatgg cgcaaccaat
     8041 atgcagattt tttggaacat tacgatacct caattaaaac cgattatctt gtttactgtg
     8101 atgatgtcaa ccattggtgg tttgcaagtc ttctctgaag cacaagtatt agtaccccag
     8161 ggggcgactc ctgaaggtgg ggcgatgacc atcgtttact acttgtataa tacggcgttt
     8221 acaaacaatg agtacggata tggatcagca gttgcttggg cattagtctt gatcattctg
     8281 gtcttttcgt tgatcaactg gtacttgacg acttatcgaa tcaaggggga ataaacatga
     8341 gcaatcggat ttttaaaata cttgttagag ccttcttagc gattggtgtc tttctttcga
     8401 tcttcccatt ttattggatg attgtaatgg ccagtaaaga taatagtgaa atctatcata
     8461 ttccaccaga tttgatgcta gggtctgaac tgatgaataa tatcaaaacg gtttttactg
     8521 aaacaacatt ctttagctcg ttgcttaata ctttatttgt ggcactaact tcgacactgc
     8581 tgattttgtt ctttgattcc ttagcgggtt tttcatttgc caagctgaac tttaaaggga
     8641 aggcctttct ctttactttc ttattgggaa ccatgatgat tccgggtcag ctgaatatta
     8701 ttccttcatt ttatttgatg gataaatttg gttgggttgg ttcttacaag gcgttgatta
     8761 ttccagggat ggcaaatgcc tttggtattt tctggatcaa gcagtattgt accgatgcca
     8821 tccccgacag tttgttggaa tcagcaaaat tagatggttg tacacttttt aaagcctatt
     8881 acaaagttgt attgccaatt atgaaaccag ctttagcatt tttagcactc tactctttta
     8941 tgggtgcgtg gaatgactat atgtggccat tgattatctt aaacgatgaa tcaaaataca
     9001 cgttgatgtt ggctttgaca caactacaag gactttataa aacaaattat ccattggtaa
     9061 ttactggtac gttactggca actttgccac tactcttagt ctttactatt ttcagtaagc
     9121 aattgatttc tggtatcaca gaaggtgccg ttaagcaata gaaagaggta ttagtgtgaa
     9181 aacaaaacca cttagtatcg gagatacacc caagaataga gcgcatggca aataaacgtc
     9241 aaaaaccgca ggtctgacga ttatgaaagt tatttagatg gctggctcta agtcttttct
     9301 aacaaaatca tctggcaata aaccttctga aatcagtaat ttggtcgctt gtttaattgc
     9361 tttatctttc tgtttttgaa caagattgtc tggcatatca atcttatata agtcagtcgg
     9421 attccaaacc tcaccagttg aaagcatgtg gtagatagct gtgagcatca ttcgagcaat
     9481 cgtgatgata gcccgtttct taccacgtcg tttgtaaatg cgcacgtact tcaaacggta
     9541 gtacggattc ttttctttca ccaaaacggc tgcgtgtgta catcaatacc acaacaaacg
     9601 ggataaacga ctttcatgat tcttcgcccc tttctaaaag tagcggagag gcagtgactg
     9661 gtctgggcag catttaacag gatttgttaa aacaatgatt agtgtgcgga cttctatagt
     9721 tccgctcatt tgtgcttgaa cgccccgacc ttacactggt taatatgctg gttcaaacaa
     9781 tcgaggtttc ctacaactca cctcaccgtg ctttgtagta tgcctctcct aattctatta
     9841 taaacggaaa tccgcgtctt cattactatt tgactcgagg agcggagcga gtcgagatgg
     9901 aggttaatat gaaggaaaaa tttatcgcct tacaagcaaa attaatggac ttgattttat
     9961 tgagtggttt gatcttgatc ggggcaatga cgataattgg tttcttttat ggaatcgttg
    10021 cggcgtttgt ttatctagaa actaaaagtg aagataaatg gcaagtttta aaaaaacgtc
    10081 agacaatctt gattagtctc ttattagaaa tgtcagcagt ttttgtagcc ggtatctttt
    10141 tcctaaatgc gcgtttgctt tcgcagttgc aaggcggtgc aacaattatc attagtttct
    10201 ttttagccgt ttgtaccttg atcttaattc cactgtattt cattttattg cacaagctag
    10261 cagttggaac aatttttacg aaggaaacat ttcttaatag tgtcaaggtc atcttaatcg
    10321 gttttcctac ctggtttacc caaattatct taatgatcgc gctgagtgtt ttgacttatc
    10381 tgtttccggc tttatcgatc attacagttg ggtacttgat tacccgcaca aataagatca
    10441 ttaatgtgca aaaaatgaaa ttagctagtt tgcaggaggt ctattaatga aattgagtca
    10501 acgggaaatt gatcttttaa agctggcttt agatggtgaa gaagaaacga ctgtgccgca
    10561 actaaaaaaa caattgaagc tctcagtcca tcaaattcgc tatgctattg ataaagtgaa
    10621 ccgcttttta attgaaaaaa agttgaatca aatttttatt gaaaaagatc ggctggtcat
    10681 tgaacaacga cgaaaaatca aaaaagaaat tgattttttt atatctcact cgacgccaga
    10741 gcagtttaaa tttaccgcag agcaaattga atattttatc tcattgaaac tgctgctaag
    10801 tgatcaacgt ttgccaatca attattttgt agaaacatta tcgacttccc ggacttctgt
    10861 ggtaaatgtt ttggaccgaa tcacacagga actggctgaa gaagaaatcg aattatcgaa
    10921 tgtaccacga aaaggttaca tgattgagga gtatagtttt aaacgatttt cctatttcat
    10981 cacgattttg aaaaaaatga tcaatattcg tgaaatctat agtttctact atcaagataa
    11041 tttgtattca aaagctggcg acttagtatt ttttgacctc tttgatttgg atatcctctt
    11101 tgattcttta ggggagacga ttcagtattc aaaacagatg gccagtgaaa ttgatgatac
    11161 gaacttttta tttttaacga ttttgaacta taagtatcgt gagttgatca atgcctttga
    11221 gatgactgat cgaacggaaa aattacaaat gatttcaagt tttattagtg aaatcaaaca
    11281 aaataatccg aatcaaatgg aaaacaacaa tctagccaag cgattgattc gctatatcaa
    11341 ccgtgaatta gccgatgttt ttgaagtaaa ggattttaaa gtggcctcta acgcactact
    11401 atatcatatt caacgcttta tttttagaca ggaaaatggc attccttttc gcgatattga
    11461 tagcgaagtt ctagtaaaag accacaagaa tttgtattac gtgatttttg cggcaatccg
    11521 ttcttttcaa aatgggttgt ttgaaagtgt agaagcacct gaggcggtat tgatcacatt
    11581 gtattacatc aatgaaattg aaaagaatgc accaaaagtt tttaaagcgc cgcgaattct
    11641 gattatttgt ggtgaaggcc gtgcaatttc cagcgtgtta aaaatcaaaa tcagtaattt
    11701 gatcgattcg aagtggatcg atacgatttc agtttttgaa tttgagacac ccttgatcga
    11761 tcattatgat ttgatcatta ctacggttcg gttgccggaa gtcgagtcag acaaagttct
    11821 atactcggac aatgtcttct ccgaaagttt ctttgaacaa attaaattga aactatcggt
    11881 aggtgggtac tcatttgcga cccaacgcct tggcaagctt tcacaaatca tgaatgcaat
    11941 cgtcgaagag gttaacgatg atatcaatat gaatcgtctt gaagcaaaga ttatcaaaat
    12001 tttggcgaat aatttgctgc aaaataatcg tcccaatgaa caaaaattta tctttgacgc
    12061 cagcatgttg aaatatcatc aagcagcgca aacatggcaa aatgcgattc gcattagcgg
    12121 gcaggaactt ttggcaaagc actcgattga agaacaatat gttaataata ttatcagcaa
    12181 tattgaaaat tttggacctt atatgatcat tgcaccgggt gtcttgctgg ctcatgctgg
    12241 tgaacaagat ggcgtgctta ataatggctt tagtatgcat gtttttcatg aaccgattac
    12301 ttttccaggt gaaaatgttt ttccggtaag tgtgatcatg acaattgccg taaaagatcg
    12361 cacatcttat cacgttgtcg aaaggatcgt gcaatgggtc ttagatgaag aaacgatttt
    12421 ttcgttagct gatgagaaaa acagacaacg gatcgaacaa atgatccgaa atattttgat
    12481 gatgcaagag gtgtaacatg acaatgacaa cggatgaaat atctttcgcc ttgatttcta
    12541 aggctggtac agctttttca atgatgatcg aggcattggg agatgcccgc gaaaaggatt
    12601 ttgaaaaagc agaagaaaag aaacaagaag ccgaggcttt gatgaatgaa gcccataatt
    12661 tacaaacgaa tttgatcgta aatgaagtca atggcgatga taatcaaatc aatgccttat
    12721 tgattcacgc ccaagacaca ttaatgaata caatcttgat gtcgacgatt gtcgatgaat
    12781 ttcttaattt atataaggca ggagaataaa atggagaaat tatcaatttg cttagcgtgt
    12841 aatttagggt catccaccag tatcttggtg aataaaatga aacaagtggc agccgggagt
    12901 caaaaattgt cttcttttga tttaatgatt gacgccattc cagcaggaaa tatcagcacg
    12961 gaacaagtga aggattatca aattattatg ttgggaccac aaatcggaca caggcgcaat
    13021 gaaattgagc aaaaagtagc tagtatcaat attccggtaa cagtcatcga tgcagctgac
    13081 tatggcagta tgaatgccgc taatattttg aaagaagcaa tttatcaaat caaaactgct
    13141 aattaaggat ggatagccgt gactttagat tatatcaatc aggtagttgg aaaaatccaa
    13201 acaaaaatga ttccgacttt aggtaaaatc gaaggaaatc aagtttttaa aagtatgaaa
    13261 aaaacttttt tgtaccttaa ttatgtctta ttgatttcct cggttttggc tattttgaaa
    13321 cttgtaaatg aaaagtttat ccaccaacaa cagattgctg atttgacgtc taagttgttg
    13381 ttattactga tggataattt tggctggttt tttctaggga ttctttgctt tttgatgttt
    13441 caggaaaaag gaaagtttca ctactatcta tgcagtgcgg ctttgtatct catgttttca
    13501 gcaaaagatc tgaatttatt atcagcctct aaaattgaaa cactattttt agcattgttg
    13561 gctctgttag ttagctttgt tctggtgagt ggttatcaat tagtgctgcg gggaattaaa
    13621 aagatcatca agacaccaat ggaattttta gataatttgt tactgatgat ttatttcagc
    13681 gtgattttca tggttatcta tcaaatattg tctcagctta aaggaattca tttagaaaat
    13741 attttgagtt ggttaaacat tgataaccca atgatggttt ttgtcattgt ttttcttgaa
    13801 atgtttcttt ggtacatggg catcaatggc tatggcatat tagcgccaat tgttttacta
    13861 tttgccatta gtaatttgaa tgcaaacttt caagcaatcg ccacgggcga ggcaccgcag
    13921 tttattttca cgcctaattt ttgggattat tttctaagtg ttactggatc aggaattact
    13981 ggcgccattg tgattttatc tttgttgagt aagaaaacaa cattaaagaa tctcggtcga
    14041 acagccgcta gtggaacctt gttttcagtt tccgaaccaa ttgtttttgg gataccggtg
    14101 gtgatgaatc catacttttt tatccccttt gtgattggga ctccgatttt aggggtttta
    14161 tttagtttta gttttactaa agtaatggat cttttgatgg aaaaaggtgc ggatccccgc
    14221 aaatttttt
//
LOCUS       DACCCR010000069        14117 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCCR010000069 DACCCR010000000
VERSION     DACCCR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.076"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..554)
                     /locus_tag="IVK93_002088"
     CDS             complement(<1..554)
                     /locus_tag="IVK93_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP6387369.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(570..1049)
                     /locus_tag="IVK93_002089"
     CDS             complement(570..1049)
                     /locus_tag="IVK93_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP6387370.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1055..2079)
                     /locus_tag="IVK93_002090"
                     /pseudo
     CDS             complement(1055..2079)
                     /locus_tag="IVK93_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2096..3202)
                     /locus_tag="IVK93_002091"
     CDS             complement(2096..3202)
                     /locus_tag="IVK93_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP6387371.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3431..6028)
                     /gene="adhE"
                     /locus_tag="IVK93_002092"
                     /gene_synonym="adhC"
     CDS             complement(3431..6028)
                     /gene="adhE"
                     /locus_tag="IVK93_002092"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP6387372.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6333..6713)
                     /gene="yajC"
                     /locus_tag="IVK93_002093"
     CDS             complement(6333..6713)
                     /gene="yajC"
                     /locus_tag="IVK93_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP6387373.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6810..7955)
                     /gene="tgt"
                     /locus_tag="IVK93_002094"
     CDS             complement(6810..7955)
                     /gene="tgt"
                     /locus_tag="IVK93_002094"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP6387374.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8173..8871
                     /locus_tag="IVK93_002095"
     CDS             8173..8871
                     /locus_tag="IVK93_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6387375.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9039..11678)
                     /locus_tag="IVK93_002096"
     CDS             complement(9039..11678)
                     /locus_tag="IVK93_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6387376.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12297..13328)
                     /gene="queA"
                     /locus_tag="IVK93_002097"
     CDS             complement(12297..13328)
                     /gene="queA"
                     /locus_tag="IVK93_002097"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP6387377.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13521..>14117
                     /locus_tag="IVK93_002098"
     CDS             13521..>14117
                     /locus_tag="IVK93_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6387378.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3957 a         3096 c         2544 g         4520 t
ORIGIN      
        1 gcgcctacga gataacctcc cataaacaaa gcagagatcg aagcaccacc agcaacaagt
       61 gaatacatga taaaagcagt acttggagga atgaccatac ctgtcggagc tgacgcgata
      121 ttgaccgctg tcgcaaactt ccggtcatat ccttcttcca cttgctgagg gatcaacact
      181 ccaccaatcg cggtagaagc ggcaatggcc gagctagaga tggaaccaaa taaagcattc
      241 cctaacacat tcgtatgagc caaagccccc ggcacgcgcc cgacgaacaa catcgcaaaa
      301 tccactagtt tcttagctat ccctccatta ttcatgataa tgcccgagag aacgaaaaac
      361 ggaatagcga tcagcgaaaa actattgata cttcccacca ttttttgagc tgaactaaaa
      421 gcagaaacat caaacggtat aactaacaat aaagtcatca tcgatgagaa tgcaatactt
      481 atagcaatcg gcatccctaa tacaagcaac acaacaaata caaaaaataa cactaatccg
      541 gcttctattg ccattttatt cctccatttt tatatttttt cttgaattgc tggcttgttg
      601 cttaatgtat cgattagatt gatcaggcta tagataatga tgagtacccc cgatacaggt
      661 actgacagat aaacatatcc cataggaata cttaaagaag gcgagatctg ccccattgtc
      721 aatgtgacag cattccatcc gccatacacc atgatcatcg cagcaaatac cagaaataaa
      781 ctttgaacaa caacttctaa aattttactt tttttgccag ttaatttttc acttaaaacc
      841 gttacggcta aacgtgcttt ttgtcccaca acatacgcgg aagacaacat ggcaaaccag
      901 acaagaccga atcgaacaag ctcttccgtg accgtatttg gatttcctaa aaccgttctg
      961 gaaaatactt gccataaaac aacagcgacc atcccaacaa gcaaaacagc tcccaggatc
     1021 tctaatacct tgtttaaaat cattttcatt acagctacgc tccttgctct tggatctttt
     1081 gataaaggtc tttgtaggta ggatcgtttt tgaatatatc atgaagtggg gcaactgcct
     1141 ctcgaaacgg tgtattatct acatcattga attggactcc atgttcttct tgtgctaaag
     1201 ctttttcttc ttcaattgct tttgcaaaag cttcaatttc aaaaaccgtt gattctttcg
     1261 ctgcttcttc aatggctttt tcctgttcat cagttagctg ttctttgatg gcatcattca
     1321 tgatgatgat atctggcaca cgtgtatgct catcatagga atagtattta gctaccccgc
     1381 catgtccagc tgtatacaag acaaattcat tgttttctga tccgttgatc aaattggatt
     1441 gaagagacgt gtacacttca tcacttccca taggtacggg cgaaccgcct aaaagctcta
     1501 ccattttgat agcggtttcg ctttgcatca cacggatctt ctttcccttc aaatcttccg
     1561 gcgtatggat cggaccgtct accatataaa agctgcgctg accagaatca taataggtca
     1621 atccggtaaa tcctaaattt ttggtcgatt ggtagattgg ctccattatt tcttcattat
     1681 ccatcacctt aaagaaatgt tcttcatcat tgaatagata aggaacggaa aaaatcgaat
     1741 aatctttaga gaaactttcc aacgctgagg cacttacttt tgtgaaatct actgctcctg
     1801 actgtgtcaa ttcgatcagt tcacgttctc cacccagttg tccatctggg aaatactgga
     1861 tctgtacgga accatctgtt ttttctttta ctaaacgccc aaattcattc attgcatcaa
     1921 ttactggctg ttgcttgcat aagcaaagcg caaggtaact atctcattta cgtctgcatc
     1981 tgcgacagtt gccacactag aattatttcc gcagccgctt agacctagca gacctaggct
     2041 aagcgaactt gctgcaaaca gcagtgatgt ttttttcata tgggtttcct ttcttttaac
     2101 aataagagac gagcaattct gtcaaacaaa gcaccgttaa cgactgtcca taaggcattg
     2161 ctgtgattcc gatattccga taaaaatcta aactgtcccc cattcctgtc ccgattgaaa
     2221 cgttttgaac ttctcctttt tcatcgattt gttcaagcaa tccttggata gcacgataag
     2281 cgacttcttt atattcttgt ggtaaataac gtttatggac cgctttcaaa ataccgtaag
     2341 caaatcctgc agttgcagaa gattctacgt aagaagaagg atcatccaac aacgtatgcc
     2401 ataaaccgct ttcagactga taagttttta atgcacgcac ttgtgcttca agtgtttcga
     2461 tcaataattt gcgcaatgca tcttctttcg ataattctaa gatttcaatg atctctggaa
     2521 tagcaatcgt caaccagcag tttccacgag cccaaaatgc ttcggcatag ttatgatttc
     2581 cttcaaacgt ccagccgtga taccacaatc ctgttcgttt atccatcaag tatttgatat
     2641 ggatcataaa ctggtaacga gcttcttcta aatattctgg gcgattcaac agtttcccga
     2701 tttttgccaa aggaagcact gtcatcatca atgtatcgtc ccacaactga ttcttatttt
     2761 ccggtccata tgtggcatgc tgtaacccgt cttcatttgt ccgaggcatg tcattcatga
     2821 cccattctgc ccattgttct aaataaggca aatagcgtga atctttcgtg tcttcataca
     2881 agtaagccat agtcaatagc ggtgccattg tattgacgtt tttgggcgga gcgccttctt
     2941 ccatacgatc ctcaaaccat tcattgacca cttgataagc gcgaggattt tttgtcatct
     3001 ggtaattttt ataaatacca tatagaccga caccttgtgg ccaattccac actgtccagc
     3061 ttttgtcatc tactttcagt ccatcaaagt ccaacaaaaa ttcaccatta tcgtctttga
     3121 tcgttgtcag gttttcaacg agcaggtcaa ttttttcttt cactaattct ttaggtacaa
     3181 aaatctcttc tttaataatc atctttacgc caccttttta aacgctttca ttttatatcc
     3241 ttaatatagc agtaataccg tccactataa atatcgcttt ttgaacaaaa actgtctaaa
     3301 attgacctaa atgttaaata acaaaagtaa ctttcagtta cttggaaatt aaaaaaagaa
     3361 cttggagaaa caacgttttc caagttcttt ttcatctatt ttcttatctt ttttctacgc
     3421 tttcttttgc ttatccgttg taagcatcaa tgatgatctg tttcaattca gagatcaatg
     3481 gttctttcgg attagctgtc gtacattggt cttcgtaagc taattcagcc atacggtcta
     3541 cggttgtatc tagtgtttct tgtgtgactc cttgagcttt tagactcatg ttgatgccga
     3601 cagattttcc taattcataa acagccgttg ctaaagcttc gactaattcc tctgttgttt
     3661 cgcctttcag acctaagaat tttgcgatat ctgcgtaatc tgtatctgca cggaaatagt
     3721 catatttagg gaacatcgca tgtttttgtg gatcttttgc attatagcgg ataatatgcg
     3781 gtaaaagaat cgcatttgtc cgtccatgag ggatcccgta ttccccgccg attttatgtg
     3841 cgactgagtg acaaatacct aggaatgcgt tagcaaacgc cataccagcc attgtagaag
     3901 cgttgtgcat tttttcacgt gattccatat caggtgtttt tactgatttt tctaaatagt
     3961 caaagactaa cttgattgct tgtaggctca atccacgtgt ataatctgaa gccatgactg
     4021 aaacatagga ttcgattgca tgtgttaaca catccatccc ggtatctgct gtgacagatg
     4081 ctggaactga catgacgaat tgaggatcga cgattgcgac atctggcgtc aatgcataat
     4141 ctgctaacgg atatttcaca tgtgtttcac tgtcagtgat gaccgcaaat ggcgttactt
     4201 cagaaccagt ccctgatgtt gtcgggatac atacgaattg tgttttttct ggtttgtcga
     4261 tcttatatgt ccgtttacgg atatccaaga atttttgttt tgcaccaaag aaagatgtgt
     4321 ctgggtgttc atagaacatc cacattccct tagctgcgtc cattgcagaa ccgcctccaa
     4381 gagcgattac tgtatctggc ttgaagtctg ccatcagttt cgttcctgcg tatacggtat
     4441 ttgtcgatgg gttcggttca acatctgaga agacttcgat cttcacatca tttttacgtc
     4501 gtgccaatac ttcacgaact gtatcgcagt aaccgaattg gaccatacca gggtcacaaa
     4561 cgatcatgac acgttcaacg ttttccattt tctctaagta caatagagag ttcttttcaa
     4621 agaagatttt tggcggtaat ttgaaccatt gcatgttatt tctccgtttc gctacagttt
     4681 tgacattgat taagtttaca gcagagacgt tacgagaaac agagttctta ccataagaac
     4741 cgcatcccaa agtcaatgac ggaatcattt cgttatagat atcaccgatg ccgccttctg
     4801 cagatggtgt attcactaaa atacgacaag ctttcattcg aagaccgaat ttgacatgca
     4861 actcttcgtt ttctgaatgg ataactgctg tatgtcctaa tcctccaaga ttcaacatac
     4921 cttcacacaa atcaaacgca tggtccgtgt tgttagcttt gatcatggca agaactggag
     4981 aaagtttttc tcttgatagc ggataatctg ggcctacgcc ttccaattca gcaatcagca
     5041 ttttcgttcc ttttggtaca ctgattcctg ccaattttgc gatttccatc gcagaatgtc
     5101 caacgattga tggattgacc gcatattttc cttcgttcat cactgcatct tctaattttt
     5161 gcaattcatc tggttttacg atataaactt ggtgtgcttg gaattctgct tttactgctg
     5221 catagatttc tttatccacg atcacagctt gttcagaagc acaaatcatt ccattatcaa
     5281 atgttttcga aacgatcaaa tcgttcactg cacgtttaat ttttgctgtt ttttcaatat
     5341 aagctggtac gttacctgga cctacaccta aagctggttt tcctgtagag taagcagact
     5401 tgaccattcc agctccacct gtcgctaaga cgatcgcgat acctggatgg ttcattagca
     5461 tagaagtcgc ttcgatcgaa ggatgttcga tccattggat acaattttca ggagctccgg
     5521 cagcgatagc agcatcacgt accactcgtg ctgcttctgc agaagatttt tgggcacttg
     5581 gatggaaagc aaaaacgatt ggatttcttg ttttcaacgc gatcattgat ttgaaaatcg
     5641 ttgtggaagt cggattcgtt gttggagtta ctccgcaaac cacaccgact ggttcagcaa
     5701 tttcaatcaa ccctttttgg acatcttcat tgatcacacc aactgtttta tcatgtttga
     5761 tattatgcca aatatattcg gacgcataca tatttttgat tgctttatct tcgtaaacac
     5821 cgcggcctgt ttcctctaca gccatcttcg ctaatggcat atgttgatcc aaagctgcca
     5881 ttgccatttc atgcacgata tggtctactt tttcttgatc aaagctttcc atttctttca
     5941 atgctgcatt tgctttttct gccaaatcat caatcattgc ttgtacgtca attgtcttcg
     6001 ttttttcttt ttctattact ttagccatca ttatcctcct aatgggtcat ggttatttgt
     6061 taaataattc acatgtatat catacaatat ttatttttct atgtaaacac ttttttgtga
     6121 tttttttcac agttattttt aaatataaaa ttgctttatt tacaggtgtt tgtatagaaa
     6181 aatatataaa gttatttatt attatttata tgacacaccc gattattcaa tattgagcaa
     6241 tatatcccat gctcttatct ctttcttttt tgaataaaaa atgaggtagg acatctttct
     6301 gccctacctc gtctttaaat aagctttgat acttattttt cttccttctc ttctgtctct
     6361 tccagcgttt ctgttgtttc agtttttact tcaactgtct cagctggttc ttctgttgtt
     6421 tcaacagtag tcgttgcatc atttgttacg acagtccctg gtttgaccgt tttgattgct
     6481 gcacgatcgt actcaaggaa gatcccttcg cagtctaaaa caactgtccg tttgtcattg
     6541 tcgatttctg aaacaactcc atgtaatccg ccgatcgtta cgactgaatc acctggtttc
     6601 atgctatcta acaagttttg acgttcttgc tgttgtttct tttgcgaacg tgacataaaa
     6661 aaccacattg ctaatagcgc gataaacatc acaagcattg gtaatgttcc catttttttc
     6721 acctcactaa ttctgtatct acaaaaaaca ctttagcaga aaaatgctga taacactagt
     6781 tttttactta atttttaaaa gattttcctt tagaagcttt ttgcgttttc tttattgaac
     6841 ccgtattctt caaaaaaggc ttctctaaat tcaagcaaat tatcatccat gattgcttga
     6901 cgtacttgtt tcattagatt caacaagaag tataaattgt ggtaagaagt caaacgaatc
     6961 ccaaatgttt catcacattt gatcaaatgg cggatatagg cacgtgtata gtttttacat
     7021 gtataacaat cacatttctc atcaattgga cggaaatcat gggcatattt cgcatttttc
     7081 actactagac ggccttgaga agtcatacat gtgccatttc gagcgattcg agtaggcaag
     7141 acacagtcaa acatgtctac tccacggata acaccatcga tcaatgaatc agcagcaccg
     7201 acacccatca agtaacgtgg tttgttttcg gggatcaacg gtgtagtgaa ttctaataca
     7261 cgattcattt ctgatttagg ttccccgaca gataacccgc cgattgagta accagggaaa
     7321 tccatcccga ctagatcttt cgcgctttga cgacgaagat cttcaaaccc tgctccttgg
     7381 atgattccaa ataatccttg acggtctgga ttagcatgag ctttcaatcc tcgttctgcc
     7441 catcgagacg tacgttctac tgattttttc acgtagtcgt aactttcatc gaatggtgga
     7501 cattcgtcaa aactcatcat gatatcagag cccaatttat tttggatgtt gattgctttt
     7561 tctggtgata aaaacatacg cgaaccgttc aagtgatttt tgaaatgcac gccttcttct
     7621 tcgatattcc gcatatcact taatgaaaaa acttgaaagc cgcctgaatc tgttaaaatc
     7681 ggttgatccc agttcataaa tttatgcagt ccgccagctt cttctactaa gtcttcacca
     7741 gggcgtagcc ataaatgata tgtattactt aaaataacgc cagcacccat ttctttcaac
     7801 tcttctgggg acattgtttt tactgtagct aatgttccta ctggcatgaa cattggtgtg
     7861 gggaaagttc cgtgtggcgt gatcaattcc cctaaacgcg cgccagtatg tttttctttc
     7921 ttgattaaac gatagcgaat ggcaggttgc gtcattcatt tccctacttt ctatataaaa
     7981 tatcttgaca actcccacca tcataatcgt ttcaagtgct ttttgcaagt tccctcggct
     8041 tccttctact ttatttaata ttcttttttt tatccttttt ttatgaataa caaaaaaaag
     8101 aaaaacatta tgacagaaat attttttgat acatggtata ttggtgttat taaagaaaag
     8161 gagggaaaaa tcatgcaatc ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa
     8221 ggacgttggg gacaagctgt tttactaaat ctaatcccta cattgattgg tattgctact
     8281 attcttctta ttatgattcc tattatcttg tttgccacca ccatatatga tggatcagcg
     8341 agcgtctcag aagcgacaac ttcaggaggc ggtagttcag gaggcggtgg cggaattatt
     8401 tccagcatca ttagcgcttt gttcatgagc ggtatttctt ggacttatct tgatatttta
     8461 cgtggacaaa aagatcagat cgaaccattc aaagatgcat ttcgtggatt caaaggtttg
     8521 tttatcggcg gagtgatcag cttagctgtg ctgatctcga tctttacaac attatggggt
     8581 ttgctattag ttattcctgg aatcattaaa agctattctt attctcaaag ctattttatt
     8641 tactacgata tcgtccaaga aacaggacaa acacctaaag tactggatac gatcactgca
     8701 agtcgaaaat tgatggatgg ttataaagga caacttttct ggttagatct ttcatttatc
     8761 ggctggcata tcttagcatt agcaactgct ggtattggtt atctatggct cagcccatac
     8821 atttctgcaa caaaagcagc attttatgaa caattgccaa aagatattta aaagagagtg
     8881 tgacttgtga ccgagggggt ggcaggagaa caccgtttat cgagaggcag taaaagaaac
     8941 attcagccca ttagcaaaaa ggaggtgtga caacattcat gtcacacctc ctttttgtat
     9001 agttccagta aaaagaaaat caacttagaa acagttaatc attcctcttc ttctgcttcc
     9061 ggtaatcgat ggaacttgat cagttcattc accgcgattg gtacgataga taaaatccct
     9121 accaagaacc agtgattcaa actgatgtgt actaggccga atagttcttg cgtaaatggc
     9181 agcaaagcaa cagccgttgt aatagctaat gccagtaacg ccatttctac caaagcttta
     9241 ttggtcgcta aattgacttt gaagatcgat tggctgctgc gtacattgaa cacatgcaga
     9301 atcgatgaat aagctaagat caagaatgca acagtttgac cgacatgctg acttgcgtct
     9361 acccatgggc tgatagtatc gacataacta cctaaataat aaccaaacaa ggttacgatg
     9421 gtgaagaccc ctgcatttat ggcaattttt tgcaataaac ctcgagcaaa gatcccttca
     9481 tttttcggaa tgggtggctg atccatgatg ccgtgttccg ctggctctct tcctaaagca
     9541 aatcctggaa gtccatcagc aactacattg acaaacagta gttgcacagc tgtaaatggt
     9601 gctccccatc ctaacagcat cgcaatcaaa acaataaaga tctccgaaat attacagctt
     9661 agcaaaaagt tgatagcttt acgaatattc tgataaaccg aacgtccttg actgacagcg
     9721 tctacgatcg tcgcaaagtt gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca
     9781 tctgttcctg tgatccccat ggcacaccca acgttacttg ctttcaacgc tggtgcatca
     9841 ttcacaccat ccccagtcat cgctacgact gcacccgtac gttgccaaga tttgacgatc
     9901 cggatcttgt cttctggcgt tacccgagca tagacagaga gagctttgac acgcgcatct
     9961 aattcttcgt ctgataattg atgcagttct gatcctgaaa gtgcttctga cggatcattc
    10021 aagataccta attctttcgc tatggcactg gcagtcacca catgatctcc agtgatcatg
    10081 actgttttga tgccagcctt tttcgctcgc ttgattgctc ctttactttc tggacgtggt
    10141 ggatcgatca ttccgatcag accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa
    10201 atatcttgcg gttcttcgtc ataagtagca taaccaacag caatcactct cagtgcccgt
    10261 ttaccaaagc gatcattaac gatcgccgct tgttctacat cgcctgaact aaagcgcgga
    10321 gcaagaacat cgaatgctcc tttggtgatt gagatgtatt tttcgcccat ttggtgaact
    10381 gttgtcatca tttttcgttc agaatcaaaa ggaagctcgt ttactcgtgg atatttttct
    10441 tctaattctg cctttgtatg ataattctct tcaacagccc ttacgatggc tacttctgtt
    10501 ggattacctt tgataacaag gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt
    10561 gcagccattt tcaatatttc catcgcgctg ttcgtcattg aatcttcgat attcgttact
    10621 tcatcttctt tgctccatac ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt
    10681 ttgtctgagc agataacatt agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg
    10741 atcgcatgtt tcctcgccat cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca
    10801 gtcaaggttt ccggtacagc tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg
    10861 agtggttctc cttgcagttc accaatcaca aatactaaag ccgcagccgc taaagcgatc
    10921 atactgattc gtttgcctag ctgattcaat cggacctgta aaggtgtttt ttgcatagta
    10981 ttttcgttca aaagatccgc gatcttaccc atttctgtag ccatgccaat ggcagtgacg
    11041 atcgctttcc cacgacctgc agcaaccgta catcctttaa ataccatatt aagttggtca
    11101 cctaatgact catcttcttc agcaataaat gctgcatctt tttcaactgc ttcactttct
    11161 ccagtcaacg ccgattcttc tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca
    11221 gaaatagcat caccggactc taaaacaaga atatcaccca ctaccagttg ctctgactca
    11281 acagtggtca ctttgccgtc tcgtatgaca gttgccgttt gtttattcat atcttgcaag
    11341 gattccagtg ctttttctgc gtttccttct tggacaatcg ccaataccga gttgatcaca
    11401 acgatagcga tgatcagcag tccttcaaat aaatccccat gttctgtagc aaatgcggta
    11461 taaaaggaaa tcaccgctgc tacaagaagg atgatcgttg taaaatcgga caagcttcga
    11521 aagaattttt tgatcatcga ttctttcggc gcctcatcga atttattcaa tccctgcttt
    11581 tttcgccgtt catccacttc ttgtgaagac aacccagtat ctggatctgc ttctgttacc
    11641 ttctttactt cttctattgt ctgcttgtat tcttccatcc tattcctcct ttaaaatctt
    11701 cgtacaaaaa aaggtactta ttttactata acatcctttt tttatttact tcaaataaag
    11761 ggcttatcga ttatttgtta agaaagcgta aaatttaaag tgcttatatt tgcaaacgct
    11821 accaaaggat gctattatat agatgtaagg aaggaggata ccatcaaata gacagaagaa
    11881 tttctcgata gaacagtgta agcaaacaaa gttgttagaa gtacaggttt cacccagaca
    11941 tgcgtgtaga gtacaaacct ataaatagtg aatgcgtatg gatgtttgga tcacgaagta
    12001 tccgaaacaa gaagtaatgc gatacgtaac aattgaataa cgtaaacgtc tgaaaattga
    12061 ataggaacaa gagatgtttc aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac
    12121 atgaagaaat tcaacaccgt gctttgcatg tatgatggaa agttacaatc atttctgttt
    12181 atttgaatcc ttccgaggta caagaaagcc gagcttatcg agagctcggc tttttctttg
    12241 tcataaaatc tttgtaaagc ttttttgtta ttcaaaacta gcggcttttc ataaccttat
    12301 ttcacgaaca tcgcatctcc aaaactaaag aaacgatagc gctcttcaat cgcatgctgg
    12361 taagcagata aagtcaattc tcttcctgca aaggcactaa ctaacatgac taaagtcgat
    12421 tttggcaaat ggaaattagt tgagaaagct tctaccactt tgaattcata gccaggcgta
    12481 atgaaaatat cagtccatcc gctgtctgct ttgatttctc cattaaattt cgttccaatc
    12541 gtttctaatg ttcgaattga agttgttccg acagccacga ttttcccgcc atttttacgt
    12601 acttggttca atcgctcagc cgcttcttcc gtcaaacggt agaattcact atgcatatgg
    12661 tgttcttcaa ttttatctac gctgacaggt cggaatgtac ctaaccctac atgtagtgtc
    12721 agatagacta attcaacacc ctttgcttcg atctcatcaa gtaattcttt cgtaaaatgc
    12781 aatccagctg ttggcgctgc agcagaacca ttttctttcg catagacagt ttgatagcga
    12841 tctgggtctt ccagacgttc tttgatatat ggaggcaatg gcatttctcc caatgattca
    12901 agaatctcta gaaaaattcc ttcatactca aagtcaatga tccgtccgcc gtgttccaat
    12961 tcttctttca ctactgcttg caatctgcca tcaccaaaag agattcttgt cccaactttg
    13021 gcacgtttcg caggtttgat caatgtctcc cacgtatctc cttgtgtatt cgttagaaga
    13081 aggacttcta aatgcgcacc agtatctggt ttttctccat ataaacgagc aggcagaact
    13141 cgtgtattat tcataactag cgcatcacca ggctgtaact cttcaatgat ttcatggaaa
    13201 tgtttatctt ccatctctcc agttttccga tccaaaacaa gtaaacgaga atgatcgcgg
    13261 tcttttaatg gtgtttgtgc gattaattct tccggcaagt caaaatcaaa atcttctgtt
    13321 gttaacatat cgtcactcac tttctatcat gtgcttcatc attctatcat gtttttcgtt
    13381 ctgttggcaa aatcagcact ttttcacttc tattttttgt tttcctaaat aaaaaaacta
    13441 ccctatttgt attttttcat tgttttaggc atactagtaa tagatagttc ttcattgaaa
    13501 atagaaaaag aggtggcacc atgattgaat ttcaaaatgt gtcaaaaatt tataaaggtg
    13561 gaaaagtcgc tgtagaggac gtcaatcttt catttgaaaa gggtgagttc atttgtttta
    13621 tcggtacgag cggaagcgga aaaacgacga cgatgcggat gatcaaccga atgactgatc
    13681 caacacaagg gaaaattttg attgatggaa aatccatcca agaaattgat ccagtggaat
    13741 tgcgacgtca gatcggctat gttatccaaa gtattggatt gatgcctcac atgacgatcc
    13801 gtgaaaatat cactcttgtc caaaaattgt tgaaagtcag tcaagaagaa cggaataaaa
    13861 cagctgaaaa aatgattgat cttgtggaat tgccccgtga gatgttagat cgctacccac
    13921 atgaattatc tggtggtcag cagcagcgta ttggagtcgt tcgtgccctt gcagcgaatc
    13981 aagatatcat cttgatggac gagccattcg gtgcgcttga tccaatcact cgtgattcct
    14041 tgcaagacct tgtcaaagat ttacaagagc gtttgggaaa aacgatcgta tttgtaaccc
    14101 atgacatgga tgaagcg
//
LOCUS       DACCCR010000070        13817 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCCR010000070 DACCCR010000000
VERSION     DACCCR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.077"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IVK93_002099"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IVK93_002099"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP6387379.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IVK93_002100"
     CDS             complement(2261..2512)
                     /locus_tag="IVK93_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP6387380.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IVK93_002101"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IVK93_002101"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP6387381.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IVK93_002102"
     CDS             3462..3680
                     /locus_tag="IVK93_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387382.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="IVK93_002103"
     CDS             complement(3969..4481)
                     /locus_tag="IVK93_002103"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP6387383.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IVK93_002104"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IVK93_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP6387384.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7368)
                     /locus_tag="IVK93_002105"
     CDS             complement(6934..7368)
                     /locus_tag="IVK93_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP6387385.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="IVK93_002106"
     CDS             complement(7378..8166)
                     /locus_tag="IVK93_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6387386.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="IVK93_002107"
     CDS             complement(8163..9104)
                     /locus_tag="IVK93_002107"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP6387387.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="IVK93_002108"
     CDS             complement(9281..9868)
                     /locus_tag="IVK93_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP6387388.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IVK93_002109"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IVK93_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP6387389.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11699..12709
                     /gene="gap"
                     /locus_tag="IVK93_002110"
     CDS             11699..12709
                     /gene="gap"
                     /locus_tag="IVK93_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6387390.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12794..13729)
                     /locus_tag="IVK93_002111"
     CDS             complement(12794..13729)
                     /locus_tag="IVK93_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6387391.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3811 a         3096 c         2435 g         4475 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgtaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtaaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgtattaa ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat
    11701 gacagtaaaa gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat
    11761 caaagaagtc tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat
    11821 gttagcacaa ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc
    11881 aacagaagac tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc
    11941 aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt
    12001 ttatacatct gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat
    12061 ttctgcccca gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga
    12121 tgaaaacgac aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc
    12181 ttatttctta aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta
    12241 cacttccaca caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg
    12301 ttcagctgct gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt
    12361 tatccctgag ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg
    12421 ttcattaact gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga
    12481 agcaatcaaa aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt
    12541 ttctagcgat gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt
    12601 aacaactgct ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg
    12661 atttacttgc caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc
    12721 aatcgataaa agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta
    12781 cttttaaact cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt
    12841 tttcaggatc tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt
    12901 ttggtccctt cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt
    12961 cgtctccatg tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg
    13021 cttcaccgaa aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg
    13081 gaaatgctgg aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag
    13141 aataaccgca atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca
    13201 tcataactgt tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat
    13261 tgttttctag cactttgttt atccactgta catagtcttt acgctctggc catccaaatc
    13321 caatgtaatc cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac
    13381 ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat
    13441 gagccacaag agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt
    13501 tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt
    13561 tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt
    13621 ctttctcaga aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa
    13681 caatcagagc taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat
    13741 ctcatttctc ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca
    13801 atgttcattt ttataac
//
LOCUS       DACCCR010000071        13464 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCCR010000071 DACCCR010000000
VERSION     DACCCR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.078"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..180
                     /locus_tag="IVK93_002112"
     CDS             <1..180
                     /locus_tag="IVK93_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387392.1"
                     /translation="TKKMMETIGPALSAQRAVMQNIDTEEFRRATERMKQSLSPKQAL
                     KSDYKIADDIEDSNK"
     gene            285..1196
                     /locus_tag="IVK93_002113"
     CDS             285..1196
                     /locus_tag="IVK93_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387393.1"
                     /translation="MGERTFEDVKDYVELQSQEKCTVLSAKPEQEFNDLGTEVTVWNV
                     KTDTDGDWWVVEGDNVPMNLYPQSAYYFGTDEVYSFHMGLMERMRVLHEKYDPEDFVR
                     SVTLDTEIAPQLFRKLKNVATLIDSAEEIEDFQSIGVQCREILIELGNHIFIADMAGT
                     KEQPQKSNFKKKAELFIQFYLTGSDNSDYRSIIKKLTESTWDYANKLTHSSTTTKYEA
                     STCVMLVTSLVGVYENITQKVFDPVSQYICSSCKSKKLTIDSDDSDENGMVESLYLKC
                     EECGDITKVVFLTSDKDSSYTKGKIIE"
     gene            complement(1530..2672)
                     /locus_tag="IVK93_002114"
     CDS             complement(1530..2672)
                     /locus_tag="IVK93_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6387394.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(2711..3445)
                     /locus_tag="IVK93_002115"
     CDS             complement(2711..3445)
                     /locus_tag="IVK93_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387395.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            3677..3952
                     /locus_tag="IVK93_002116"
     CDS             3677..3952
                     /locus_tag="IVK93_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6387396.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4050..6047
                     /locus_tag="IVK93_002117"
     CDS             4050..6047
                     /locus_tag="IVK93_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387397.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENIEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(6123..6458)
                     /locus_tag="IVK93_002118"
     CDS             complement(6123..6458)
                     /locus_tag="IVK93_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387398.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            6585..6800
                     /locus_tag="IVK93_002119"
     CDS             6585..6800
                     /locus_tag="IVK93_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387399.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            6837..7199
                     /locus_tag="IVK93_002120"
     CDS             6837..7199
                     /locus_tag="IVK93_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP6387400.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGSLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            7245..7571
                     /locus_tag="IVK93_002121"
     CDS             7245..7571
                     /locus_tag="IVK93_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387401.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            8414..10237
                     /locus_tag="IVK93_002122"
     CDS             8414..10237
                     /locus_tag="IVK93_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6387402.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            10251..11660
                     /locus_tag="IVK93_002123"
     CDS             10251..11660
                     /locus_tag="IVK93_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6387403.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            11678..12514
                     /locus_tag="IVK93_002124"
     CDS             11678..12514
                     /locus_tag="IVK93_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6387404.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            12579..13097
                     /locus_tag="IVK93_002125"
                     /pseudo
     CDS             12579..13097
                     /locus_tag="IVK93_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13176..13430
                     /locus_tag="IVK93_002126"
     CDS             13176..13430
                     /locus_tag="IVK93_002126"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6387405.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
BASE COUNT         4429 a         2045 c         2706 g         4284 t
ORIGIN      
        1 actaaaaaaa tgatggagac aatcggtccg gcattatccg ctcaacgagc agtcatgcaa
       61 aacattgata ctgaggagtt cagacgagca actgagagaa tgaagcagtc tctatcacca
      121 aagcaagcat tgaaatccga ttataaaatt gctgatgata ttgaagatag taataaataa
      181 agagtccaga actttttcca attaataaac tattaaaaga attagagagg tgaaataaaa
      241 ctagaaaaat acaatctatt tggtaaaaat catggaggta aataatgggt gaacgaactt
      301 ttgaagatgt taaagactat gtagaattgc agagccaaga aaagtgtacg gtgttaagtg
      361 ccaagccgga acaggaattt aatgatcttg gtacagaagt aacggtttgg aatgtcaaaa
      421 cggatactga cggcgattgg tgggttgttg agggtgataa cgttcctatg aacctttatc
      481 cgcaaagtgc atactatttt ggaacagacg aggtatattc gtttcatatg ggtttaatgg
      541 agagaatgag agttttacat gaaaaatatg atccagagga ctttgtaagg agcgttactc
      601 ttgatacaga gattgcacca caactctttc ggaagctaaa gaatgttgct actcttatag
      661 acagtgcaga agaaattgaa gattttcaat ctattggtgt tcagtgtaga gaaattctaa
      721 tcgaacttgg taatcacatc tttatagcag atatggcagg tactaaagaa caaccacaaa
      781 aatcaaactt caaaaaaaag gcggaacttt ttatacagtt ttatttgaca ggttccgata
      841 actctgacta tcgaagtatt ataaaaaaac taactgaatc aacatgggac tacgctaata
      901 aattaactca ttctagtaca actactaagt atgaagcctc aacttgcgta atgcttgtaa
      961 cgtctttggt tggtgtgtat gaaaacataa cgcaaaaagt ctttgatcct gtctcacagt
     1021 atatatgttc gtcgtgtaaa agtaaaaaac tcacgataga ttctgatgat tctgatgaaa
     1081 acggaatggt tgaatccttg tatttaaaat gtgaagaatg tggtgatatt acaaaagtag
     1141 tatttttgac tagtgataag gatagttcat acacaaaagg aaaaataatt gagtaatgat
     1201 tatggatcat gtcaaaaaca tatcacgcaa ttcaaagtgc acacgttttt aaatttgata
     1261 cctcaaataa cttctctacc tgcttttttg ggtacgatat tggatacgtt ctgggggata
     1321 tcaagtgctg tgataggctg tcagtgatat ataccagagc gggaaaaatg cttttataac
     1381 gcgaatcatg taccaatgct attgaaaaga gtcattggaa ggcagtgttg ggtaaacctg
     1441 ttataggagc tagaaccctt gatatataag ggttctagct tttttgtttt gcctgtatac
     1501 tagaaatgac cagattttga ccagctcttt tactgatgag ccatcatatc agcaaagtct
     1561 tgacctgttt tcttaggtgc attatttgta atatgtgcat agatgtctaa tgtcacttta
     1621 ctattttcat gccctaatcg ttgttgaact accttgacag attctcctgc ttctaataat
     1681 aaactagcat gtgtatgtct aaacccatga ggtgtaatac gaggcaagtt atattttttc
     1741 aaaatatatc ttaaccaatc attaacagtg tttggaatgt atagagtgtt tttaactgtt
     1801 gtaaaaacat gttgctcttt agagctagta ttgtatccaa gttttaaata ctcttctttt
     1861 tgctgtaatt tccagttgtt gaggatagaa agagtctcag tatctaaaga gataactcgt
     1921 ctagaagctc ttgttttcgg agattgaatt ataactttac caaattcatc tacagcaaga
     1981 gttttattga catgtagttc tttattaaac gtgtctatgt ctttccactg taaagcgagt
     2041 acttcactct ttctcatacc tgtgtatgcc aataatctga agaaggcgta ttgtttcatg
     2101 ttaccaaaat ctttaaaagc atccagtaaa ttattaagct cctcttttga gtaaaattta
     2161 cgatttttct catactcttt ttttcgtgga agaagtgttt tttccatagg attattgtca
     2221 atatattgca ttgcaacccc gtagcgtaaa atttgtgcag tctgttttct catataatcg
     2281 taactttcat atttactgtg ccattcatct accacttttt gacaatatgg gacagagata
     2341 ttcgatagtt taagcttacc aaatgctgga agtatttgat tatttgcgta tcttactgag
     2401 attgcgattg ttgaagcttt aacactggta cgctgatgag ttatccattc attatacagg
     2461 tcttcaaatg tcatatcagg tgctttcttt ttaacaccgt ataatatttg atgtctgtac
     2521 tcgtcataat ctttttgagc ttcttgtttg ctactaaaac ctcttctcgt agtatttatt
     2581 ttttcaccag ttcgtggatc ggtggcaatg tgtcctttat acatccacgc cacttgaccg
     2641 tttttctttt tgtactttga tatttttgcc attaaatcat ctcctcttag tttacaagga
     2701 gtacttacca ctagatggta tcacctccct tcattttttt cttagtgtat tttttcccaa
     2761 ttgatgaaat cgtttgttct agcttgttta tattatcagt aatttctctc acagcttctt
     2821 cgggataaga catgtctttc aattgaatag gtagccattc atctaccatt ctttctagta
     2881 gttcttctat actgctagtg aggaaaagaa tgttattgtc aaattcttct acaatagcat
     2941 tgatgaataa ttcttctatt tttaagatat tgaaaagact aattttttca tttttaactt
     3001 tattacaagt cttgttagca ataaattctt gcatctctgg ttctaatttg gaatatctat
     3061 ccagccatgt atctaaatta gagtgtactg gtgggaactg ttgacgaaga tgctggaaca
     3121 ggtttttatc tggaaatgtt ataccatcta gtacaatgct atctctataa agaataattc
     3181 ccaaaatata ttcttctaca ttgccacaaa ctaactcctc tatactcata tttcccaaat
     3241 cagcaattcg ggataatcgc tctggtattg gtaaataggt tcccttctcc cattcgtaga
     3301 tagttttttt gtcaattaat tgaccgttgt taatcataag accaaatgct ggttgtgtta
     3361 attttttact ctttcttata gcttttattt ttgttccgag tatctttttt cgttctaaat
     3421 aatttattgg tggacgtttt atcatattga ccacacctcc taaaattagt atacctcaaa
     3481 aaaggggtgt tgtaaataaa ttttacaaag ctgttgacac ccctttttta tcgaggtatt
     3541 atgtgtatgt cgatgaactg gtaatcattt tttgtttgat taatcggtta ataacaaaat
     3601 acttttattt tgagcaaaaa atacgaagat aattcttaga aaatttgttc aagcaatcac
     3661 gaaaggaggg ggaaatatgt ctctacaaat cattgatagc attgtttcta tacaagttga
     3721 acaacaagtg aaagagataa ttacagtgct aaagcctatg ctagaagaca ttatttctgg
     3781 agtcactcta gagtatatgg actatcagca ggcaagtgaa tatctaggtg tatcagtagg
     3841 aacgattaga aaatatgttt ctcaatacgg gttacctgtt ataaaaattg atacggtcat
     3901 tagatttaaa aaatctgata ttgatagttt catggaacgg tataaaggtt gagtaaacaa
     3961 ataagactaa aacatatagt aataaagagt ttgtttttat tatctgtatg ttttagtctt
     4021 taacagaatt ttataaggag gaaacacaaa tggaaacatc aatgattaat attccaattt
     4081 atgtagatca agaaaaatta ttagagaagc cagttacaaa aaaagaaaaa actgatgtaa
     4141 gagataggtg tttagataac tatagagaag ttacaagaga tgagtttgta gagatactaa
     4201 attcatcaca atcatttatt ccaagcaaga caaagaataa aggcaatgac aaggagagtt
     4261 ttgtggaaac cagagtaatt attctggatg tcgataacac tgttaaagat gagaagggga
     4321 aggtagtaga tttgagtaaa gatgatagta gatacttaag catagaaaag gttttatcta
     4381 tcaatttagt tcataattct gcgtttgcga tacaaaaaag tattcggtac tctgaaaaca
     4441 tagaaaaata caagattgtc tttctattaa aagaagcaat tactgactat ggtaaaatgg
     4501 ttgctgttta cgaatacttg aaaaagaaca tccccgggtg tgatgacaat gtaaatgctt
     4561 cgaatagaat gttttttgga ggatataagt cagatgcagt gataataatt aatgaagaca
     4621 acatgttaga tattactgag cttccgattg attttaccat gacatgttct aaaaattgtc
     4681 aaagtgattc agggagagag tatgaactag tcaatgaaac cgattttgtt aagttgataa
     4741 aaaatgacga caaagaagaa atgaaacagt ggtttaaaga ctgcctgttt gattcctccg
     4801 acatgtcttt taatgagatt tatgaaagat tgttaaccat tgatatgaat aaattactta
     4861 agtcaagtaa aaacttaaga tgtctgtttc acaacgatca taatccctct gctagtattt
     4921 ttaccagtaa aactgggcat tccatttttt attgtcattc cagtaattgt gatatcagtg
     4981 aaaattttat tggagttgtg atgctaattt taaagaaaga cagtcgggta gaaacattta
     5041 attggctatt agagaaccta gacttgtatc ctacacattt tcgaaaattg aaagaggaat
     5101 caaaagtact ttttgacact cttgaatcaa tgaaagacaa acttttcagg acaataagga
     5161 attatctaga ggaaatgcga cgtgtctatg atgtcttgct ttctgatgtt aatttttttg
     5221 atgatagtaa ggaaagtgtg acttgtattt tgtcggggga acaattagct aaaaaaatga
     5281 gttcttacta tggtaaaact tatgacatag ataaatgtaa taagttacta tcacttatga
     5341 cttttatagg attgataaaa aaattagatg atgaagatgt tcctttgaag atgctcgaat
     5401 acctaaatag gttaaaggaa gagaaagcgt taggaaactt taatcataag agaagcaatg
     5461 tctatcgatt agacgttttt aatgcaactt ctgtgattca aaaattgaat gaagatatcc
     5521 ttccacttct ggaaaaaatt cattttacat atcaatattt ttcttatgaa tgggttaaga
     5581 tatgttttaa cgaaaaagag gctaaaagag tatttcctca aaattcccga tcagaactca
     5641 gcaaggaaaa acaattgatt ttagatgaag cacatgaagt tttacagggc ttatcgtggc
     5701 aagggacata tctaatgaca gagaaagaac ttattaacac tgtttgtgaa cgcctacgta
     5761 atgttgggga taacagcgtg aaaaatgctt taataaaaaa cagaggctac tttgttaaac
     5821 aaggattcac tcttgaacga gcttctaagg atattaagaa actatacaat gtgaaagcaa
     5881 gtagtagttt tttaatatat ttagcgaaag gggcaggtat agatgaaaca ataaaagaat
     5941 tgatattatc aggagctggg atagttcctc tgtatcaatt tacaagtgta acaagagatg
     6001 atggacggag agtaaggaag agaaaagacc caattgtatt aaactagaca ggttatccat
     6061 agctcttaat cttatttcat ctcaatacac ccaccgttaa agttcggtgg gttatttatt
     6121 tttcaatctt tcaatagctg attgcgttca ttttcttgcc tttttaatag cttcatatag
     6181 tcaattgttc gaactgcatc ttcagtgcta agagaacgat agagagccaa aaactcttcc
     6241 tcataatcta cgacaacatt gtctagaaaa tggcttacag gggttcctag ggctctagaa
     6301 agagctgtta agctatcgag gttaggctct gttacagaat tttcccatct tgctactgcc
     6361 tgttgtgtag agaagatttt ttcagctaat tctttttgtg tccacccgag agcttttcgc
     6421 ttttctctaa tgacctttgc atattgtttc tgtttcatta atacaccccc atctcagtat
     6481 agtacaacaa gttgttgtat ttgtaatggt atttgaagtt ttttatattt tatgagttga
     6541 aatacatcat attgttgtat ataataaaac aggaggggag aatcatgaaa aaagtaagta
     6601 atctcagccg atacagaaaa gaaaaaggac tttctcaaac ggagttagca aaaaaaatga
     6661 atgttacaca gcaatgtgta agttcgtggc aaacagggag aacgattcct aaaccctatc
     6721 aaatgaagat gctttctgag atattaagtg taccaatgaa tgagctgttt tctgatgtat
     6781 ttaataaagt aaacagttaa ctaataagca attgaatgat tttaggagga gaagacatgt
     6841 attgtagaaa atgtggcaaa gaaataacag atgaaagtaa gtattgccag ttttgtggag
     6901 cactacaaga aacagatgag acaaccggaa cagacacagt aaagaaaagt actgagcaga
     6961 caattaatga aatgaaggaa caaatcgtac atggacaaga gacagggaag ctgtatttga
     7021 ttattggttg gatttctatg gtagtatcgt tagcatttat tcctgttgta tttggtgctg
     7081 ttgctgttat tatgggctcc ttatatcgag aaaaagatga gaaaatgggt acgattttga
     7141 tgatagcagg ggtagctgga gcaatctttg gtgtactttt agggatgtca atgtactgat
     7201 aagagggata agagcaaatt attttggaga taggagataa aaagttgaat aaagtaaata
     7261 tgaacaagaa gtttgtcatt ataggtattg ttgtcttggc tattataggt attatttttt
     7321 caaaaatgac aggtacatct ggattagaga agtcatcaaa accattggta acacagattt
     7381 tacaagaaca gtacaactta aatcgatcat gtgatgatgt gacgattact catgaaaatg
     7441 gagataacac ataccgggct aaagctatat tagacaatgg aagtgctatc aacataaata
     7501 ttgagtacta tccaaagaaa gaccgtatat atgtggagat accatatgct gaggtgttaa
     7561 tgttaaattg atttaaatgt attatgcata ccgtccaaac aggaactccc aaaggtgtaa
     7621 attcactagg gagtttttat agtttcctat aaattaaata ctaatacagg tgacaaaaaa
     7681 atattacaat gattgtcggg aatttttatg ttattcactg ttataacaat ataaaagaaa
     7741 tgaaaaagaa aaaattatta tggatatgtt tggtattttt attgataatt ggtggaattg
     7801 aaatacttac tacatgtaga ttagaaataa atcagtttaa acatttgcta gttaattttt
     7861 aagaatacta gcccaaatca ttctatcgat acctttatat ctcggaatga ttgaaattgg
     7921 ctattgctta ctgattttta ccaaaaaaac aagtatcact attgttgcct atattgttgg
     7981 aatgctgttt attccatcct ttactcacca aattcatcaa tttttcccaa gagcagaatt
     8041 gttgaagttg tgtgatccgt tatatgcatt tttgatgctt tcacgatttt gggaatttcc
     8101 cgctggttat gtaggaatgg ttttactggt atagttaata ttagatatac ttattttagc
     8161 agtaagtata cgcagatata ccctagttga tgtgatctaa ctgttatatt accctatacg
     8221 gtcaaacgaa aagataaaaa attaaattcc ctgttgattg cgctatcata atgtgctatt
     8281 attggattac agcaaaataa cgttattggc atgttactga ttatgcaggc aagaccaaga
     8341 caaactaaca cttcaatgtt gattggggcg tggtttgact tggtcttttt tactgcttta
     8401 aggagagaga ataatggatt acagagaagt aggaagaaat gtattgcaac aggttggggg
     8461 aaaagaaaat gtggtcagct taacccattg tgccaccaga ctgcgttttg aactcaaaga
     8521 taaaaggaaa gccgatacga aagccttaga aaaaacactt ggtgtaatta gtgtggtgga
     8581 tagcggtgga caataccaag tggtgattgg caatgaagtc caaacagcct tcaaggaaat
     8641 tcaaaaggaa gtgggaactt caattaaaaa gggcccctct tcagacaatc aagaaagtat
     8701 tatttcaaga ttgattagtg tcatttctac cacctttaca ccgatgattc cagctataac
     8761 aggtgccggg atggtaaaag ccattttggc cgtcttgact ttgactggtg tactatcaga
     8821 cagtagtcaa acttacatca ttttgaatac tattgctgat gcagcctttt attttatgcc
     8881 agtactatta gcctatgggg cagccataaa gtttgatgtc aacccaattt tggcgattac
     8941 tgttgctggc attttgcttc atccaaatat ttcacagctt ttttctactg gcgatgccgt
     9001 ttcttttcta ggaattccgg tattagctgc agattatgcg ggatcagttt taccgattat
     9061 ctttactgtt tggatcatgt cctatgtaga gccctttgcg gatcgaattt cgccttcgtt
     9121 tattaaattc tttactaagc cgatgctggt atttttaatt atggggcctt tagcgttggt
     9181 tgtcattgga ccgtttggaa cgttgctgaa cgatcttata gcaatggggg ctgaatggat
     9241 taacggcaaa gcagcttggt taattccatt tttgatgggt ggtttgcagc catttttggt
     9301 ggttacgggt acagcttggg caatgacgcc aatcgctact atgcaattga gcagtacggg
     9361 ttctgaaatg atcaatgggc caggaatgct ggcttctaat attgctcaat caggtgctac
     9421 ctttgctgta gccgcgaaaa ctaaaaacaa agagttgaag cagctagctg tgtccactgg
     9481 gatcactgct ttaatgggaa ttactgagcc ttcactctat ggagtaacct tgaagctgaa
     9541 aaaaccatta gttgcttcca tgattggtgg tgcaattgct ggtttgtatg ctggcttgag
     9601 cggtttagtc agatatgcct ttgtttcccc tggcttagca gccttaccag cctttattgg
     9661 agaaaatcca atgaatattg ttcacgcaat tattacgtgt gtaatcgcct ttgttgcaac
     9721 ctttatcatt gcttatctaa ttggatttga cgatacttct gatgaatcag agcaaacggc
     9781 aatagccgaa gcagtcattg ttgaaaatcc ggtaaaagga accgtgtgtc ctatttctga
     9841 agtgaacgat gctgtctttt ctaagggact attgggaaag ggcgtagcca ttcatccaga
     9901 agaaaacagc atttattcac ctgttgaggg agaagtcatt actattttag aatctaaaca
     9961 tgcgattggt ctacgttcta aagaaggtat tgaattattg atccatgtgg ggatcgacac
    10021 cgtgaattta aaaggggaac cctttgaagt atttgtaaaa gcaggagatc aggtgtcagc
    10081 aggacaatta ttaatcaacg ccgatttcgc tgccattaga aacgctggct acgatgatac
    10141 agtgattgtg gtagtgacca acagtgaaaa ttacgcagga attagtgaag caagcggaag
    10201 cttaaaggaa cacgaaccat tgatgcaaat tagttaagga gaggattttt gtgcaataca
    10261 aacagctttc agcatttcca gagaagtttt tatggggtgc ctccattagt gcctaccagg
    10321 cagagggtgc ctatcaggag gatgggaaac aaccgtctat tattgacaag tatcagcata
    10381 cagaaggggt agctaatttt gaagttgcct cggattttta ccatcactat aaagaagata
    10441 tacagctgtt taaagaatta ggcttaaaag cttttcgctt ttccatcgct tggacgcgaa
    10501 ttatggatat tgaaaataat aaaaccaatc aaaagggaat ttctttttat catcaagtaa
    10561 ttgatgaatg cctgaatcag ggaatcgaac cgattgtcac catgtaccat ttcgatttac
    10621 cttattttct agaggagcag ggcggttggt taaatcgagc aactatcgat tattttgtgg
    10681 actatgtaca ggtattgctt actgagtatg gtgacaaggt gaactattgg ctgacaatta
    10741 atgagcaaaa cacgatgatc cttcatcctg gagcgattgg tttgccacca agtggagagc
    10801 tgccttcaaa aaaagaactc tttcaaatta atcaccatgt tttattagct caagctagag
    10861 taattcactt gtatcatcag ttgaatctta aaggaaaaat cggtccagca atcaatttaa
    10921 cggctatgta tcaagctacc tccagtccag aagatgcaat tgcagcccat aattgggaaa
    10981 cattgagagg ctggtctttc ttagatgtag cagttcgggg aaaatacaat tatttgtttg
    11041 aaaactattt gaatgatcgt ggattatatc ctaaaattga ggcagaggat caggcaattt
    11101 tatcctccgg taaacccgat tttattgcga ttaattatta ttcaactgcc accatcgctg
    11161 ccagcaaaaa tgacggatca gatgtttccg ctagagcggg ggatcaacag atcatgttgg
    11221 gagaagaagg tgtctaccgg gcagctgaaa atccttatgt agataaaacg ccatatggct
    11281 gggtagttga ccctactgga ctacgattaa cattgaggaa attgtacgat cgctatgatt
    11341 tacctatttt aattactgaa aatggctatg gggcacccga catagttgag gaagatagac
    11401 gaattaatga ccaggatcga attgattatc tagccaaaca tattctggct attcaagcag
    11461 ctttaacaga tggtgtagat gtttttggtt atttaccttg gtcagcgatt gatgttgtta
    11521 gtacccatca aggcttcaat aagcgttatg gttttatata tgttgaccga accgatgaga
    11581 atcttaaaga attaaaacga ataaaaaaag aaagcttctt ttggtatcaa aaagtaatcg
    11641 caagaaatgg cttagcttag agagaacggt gaatctaatg agagtaatta aaacgttaaa
    11701 caacagttta attctttgct tagataagca aggcaaagaa gtaattttga tggggaaagg
    11761 gattggattt caactaaaga aaggcgattt ggttccggct gacaaaatcc aaaaagtatt
    11821 taaattagat tcactttctg atgctctaaa aaaggattac attaaactgt ttgaggctgc
    11881 tccggatgat ctaattattt gcgtcaagcg aattatcgat tatgccaacg ccaagtttga
    11941 ctttaagctg aataaaagtc tattttttac cttggttgat cacttgaagc atgccattga
    12001 acgcagtcaa aaaggcattg tttttcaaaa tcgcctgctg attgaaattc aaaagtatta
    12061 cccaaaagaa tttgagatcg gctgctatgc tatccaattg atcaacaaaa aaatgaatgt
    12121 tcagttgcca gaagaggagg ctggaaacat tgcgtttcac attgtaaatg cccaaagtga
    12181 acaagcaaat atggaagaaa ccatgctctc ggtaagagca ctaaaggata tcttggctat
    12241 gctaggatat ctgtttcccg aaaaaaaggt tgataaagat tcgatgcttt acttaagatt
    12301 tgtgacgcat ttacagtttt ttattgcccg aatgattaag aaggaagagt tgccggagaa
    12361 ggatgatttt ttgctggctt cagtgaagca gcgttttccg aaagagtatc gggcggcctt
    12421 gaaaatcaag gagtacgttc aagaagagat gggtgcctca gcaaatcagg acgaactgct
    12481 ttatctaaca ctgcatattt caagggtgta ctgaatgaag aaaatatcgg ctgcacaata
    12541 tataaagact tgttggttgt gctaaggctc ctgattcctt ggaaaattct tctaacagtg
    12601 cggaggtgaa tgtttcgacc tctaccagtg aagtggtaaa gtgttcacaa gaaactacta
    12661 gcttgccaga agagctactt ggagattggc agattaataa taaggatgct ggtatagaga
    12721 tatcagacag taaagctgta ttccgtatag gaaggtctca agtagagtca aaggattatt
    12781 atgtagatgt aaacaacaaa atgcttttac atgtctattt ttcttttgag tatgatagtg
    12841 tgaaatacat tgaagtctct attgaaagaa ctggttatag tgaatttatt agtaaaagcg
    12901 tgggaatttt aactatgcac actaaagatg gacaatatat aagtttgggt gtgaagagag
    12961 tttccggtga gaataatgag acagaagcag ttacggagca gacgatggat gaaagaatcg
    13021 attcatctaa gagtaatgag acagaagtag atactgggaa gacgatagat gaaagaattg
    13081 attcattcat cgaaactggt gttagtatca aatcttagac agcttttcgt agagacaaga
    13141 ataaattaaa cttatctacg agaggatgaa tttttatgac ccgatatgaa gaaaatttta
    13201 aacaaatgat tgttgaactg aatcaaactg gacgttctgt tcgagggtta gcgaaagaat
    13261 atggcttatc tgaagcaacg atttacaaat ggaagaattt atatttacct gatcagtcca
    13321 caggactgac tggaaaagaa gtagctgaac tgagaaaaga aaatgctcgt ttaaatgagg
    13381 aacttgaaat cttaaaaaaa gccgcagcca tattctctcg gaaaacctaa attcgcttgt
    13441 tcaatttatt gaaaaatggt gtaa
//
LOCUS       DACCCR010000072        13427 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCCR010000072 DACCCR010000000
VERSION     DACCCR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.079"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..912)
                     /locus_tag="IVK93_002127"
     CDS             complement(<1..912)
                     /locus_tag="IVK93_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP6387406.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="IVK93_002128"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="IVK93_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP6387407.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2389..3648
                     /gene="lysA"
                     /locus_tag="IVK93_002129"
     CDS             2389..3648
                     /gene="lysA"
                     /locus_tag="IVK93_002129"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP6387408.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3635..4435
                     /gene="dapF"
                     /locus_tag="IVK93_002130"
     CDS             3635..4435
                     /gene="dapF"
                     /locus_tag="IVK93_002130"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP6387409.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4438..5319
                     /locus_tag="IVK93_002131"
     CDS             4438..5319
                     /locus_tag="IVK93_002131"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP6387410.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5316..6032
                     /locus_tag="IVK93_002132"
     CDS             5316..6032
                     /locus_tag="IVK93_002132"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP6387411.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6038..6733
                     /gene="dapD"
                     /locus_tag="IVK93_002133"
     CDS             6038..6733
                     /gene="dapD"
                     /locus_tag="IVK93_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP6387412.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6749..7750
                     /locus_tag="IVK93_002134"
     CDS             6749..7750
                     /locus_tag="IVK93_002134"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP6387413.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7747..9108
                     /locus_tag="IVK93_002135"
     CDS             7747..9108
                     /locus_tag="IVK93_002135"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP6387414.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9068..10219
                     /locus_tag="IVK93_002136"
     CDS             9068..10219
                     /locus_tag="IVK93_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP6387415.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10194..11363
                     /locus_tag="IVK93_002137"
     CDS             10194..11363
                     /locus_tag="IVK93_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6387416.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11538..12917
                     /locus_tag="IVK93_002138"
     CDS             11538..12917
                     /locus_tag="IVK93_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6387417.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13198..13427
                     /locus_tag="IVK93_002139"
                     /pseudo
     CDS             13198..13427
                     /locus_tag="IVK93_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2340 c         2922 g         3681 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tggttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atatgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 attcttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaaac cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatgattca taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat
     2161 tttttcatca ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt
     2221 cgggtatttt gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat
     2281 actagacaat tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa
     2341 atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg
     2401 ataattggcg gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg
     2461 tatgatgaag aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa
     2521 cgttttgata caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat
     2581 ttagttcgtg aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct
     2641 ttgcaagctg attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag
     2701 gaactgatct atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa
     2761 gttgaattat tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg
     2821 ttgaatgtcg gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca
     2881 aaattcggca tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa
     2941 agcagctatc tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt
     3001 actgcttggt tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct
     3061 ctttccttga atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc
     3121 ccagtcgaag aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga
     3181 aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt
     3241 acaacactat acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta
     3301 gatggcggga tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat
     3361 ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt
     3421 gaatcaggcg atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt
     3481 ttagctgttt attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg
     3541 acacgaccag cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa
     3601 gcattttctg atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg
     3661 cggaaatgat tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc
     3721 ttctttatgc acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc
     3781 gttggaaatg atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat
     3841 tcgctgtttt gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt
     3901 agagacatta gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag
     3961 cgtagatatg ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc
     4021 aataaaaaac caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt
     4081 aggaacgatt cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag
     4141 aggtgaactg atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga
     4201 agtagtcaat gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact
     4261 tgcttgcggt acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc
     4321 agcagatcag gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc
     4381 gatacaaatg gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg
     4441 ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat
     4501 gctggtttgg aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt
     4561 ttaggtacaa caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa
     4621 ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat
     4681 acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg
     4741 gtcatcaccc cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca
     4801 attgcagaag cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg
     4861 tcgattccaa tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa
     4921 gaagcctcag gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc
     4981 ttcctttaca gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt
     5041 gtaataagtg tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg
     5101 caggacggaa gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat
     5161 gccttgttca gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta
     5221 ccttccggac ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta
     5281 gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg
     5341 gacagatggg aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt
     5401 tacggacggc acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta
     5461 ttgatttttc tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac
     5521 ttccgttagt cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac
     5581 atgccaaaca tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga
     5641 atcaactaat caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa
     5701 aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa
     5761 tccaagaagt gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa
     5821 accaaattgg tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt
     5881 ttgctgctac tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg
     5941 ctgcaggcgc agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat
     6001 tagaagattt attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg
     6061 ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc
     6121 aatcgaattt aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga
     6181 ctatgagacg attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat
     6241 cgaaactgct gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg
     6301 tatcgaacca ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat
     6361 gatgggagcg attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg
     6421 tgctgtctta ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt
     6481 cttagcaggt gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact
     6541 ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc
     6601 agcaggagca gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc
     6661 acgtgtatta aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt
     6721 acgcgaatta tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc
     6781 aaccggatta gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg
     6841 acgattgaaa ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa
     6901 gaaacacact gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt
     6961 ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt
     7021 tgtcatagat aattcctcag catggcggga agattcggat attccattaa tcgttccaga
     7081 ggtcaatctg gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat
     7141 tcaagcggta cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta
     7201 ttcgacctat caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac
     7261 gcaaaatgac gaggaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga
     7321 aatcgatcag cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac
     7381 ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat
     7441 cgaaaatgga catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga
     7501 aattagagag tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca
     7561 taaatatccg acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag
     7621 aaaagacact tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa
     7681 aggagccgct gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt
     7741 ggcaagatga gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa
     7801 gagcaattca aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt
     7861 gtttctgctt taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg
     7921 atttatgctt accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat
     7981 cgtttcattc aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca
     8041 cgcatacaga aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt
     8101 gaatatctga ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa
     8161 gacctgattt tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc
     8221 acaaaagcat ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct
     8281 gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg
     8341 aagatgttgc aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct
     8401 gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga
     8461 gagatggctt atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag
     8521 gctgatatcc ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata
     8581 tcaaatcacg aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt
     8641 ctttccatca ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca
     8701 atgagcattt ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat
     8761 atcggagtga tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa
     8821 ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct
     8881 gtcgttgggg gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg
     8941 aacaaattag aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc
     9001 atcggtgtac cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg
     9061 aatacggatg ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca
     9121 tgaattcaag acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca
     9181 tgaaatcggc gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc
     9241 ttttcgaaca gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc
     9301 taagcatgaa ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg
     9361 gttgactgac tttataacta aacacattga ggatcttatt tataatgtag tactgatcta
     9421 tcagccatca gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga
     9481 taaatatgaa gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga
     9541 agttttctct cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa
     9601 aggccagtct gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt
     9661 acgatttttg aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct
     9721 aatgaaattt ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat
     9781 tgtatccgga agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt
     9841 agaccattta gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg
     9901 ctatccagct gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact
     9961 acatgaatta gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata
    10021 tccatgtgta tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt
    10081 cgattttgat atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac
    10141 agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga
    10201 atttatccaa tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat
    10261 tcgatcagca aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt
    10321 ttccaactcc tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc
    10381 actataccgg aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac
    10441 agcgttatgg attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg
    10501 aagcgattgc ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag
    10561 cgcctgctta ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga
    10621 tagatacttc tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac
    10681 aatatggatc agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga
    10741 cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg
    10801 ttataagtga tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta
    10861 cttatcttcc agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg
    10921 gttggcgagt gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc
    10981 atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa
    11041 taaatggggt gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc
    11101 tggtccagca attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact
    11161 tgttttgcaa actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag
    11221 cggaacaggc aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt
    11281 atatccggat ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca
    11341 aagacttttt aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg
    11401 cggtatattc tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt
    11461 ttgattcata actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat
    11521 tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag
    11581 ggcatcaatc gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat
    11641 ttgaagcagg agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca
    11701 aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg
    11761 ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg
    11821 ttactagcgc atcagataac gaaacaaaag aatatcctta tgacaaattg attttgagtt
    11881 ctggtgtcaa accaaacaat ctgccagtac caggagcaga tctagaaaat gtctacttga
    11941 tgcgtggata caattgggca accaaaatca aagaaaaatt aaatgatccg gctattaaaa
    12001 aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc agcagaagca agccgaaaag
    12061 ctgggaaaga agtcgtattg cttgatgtga tcgatagacc tttaggcact tatttagatg
    12121 ctgagatgac agatatttta gaacaacacc taaatgaaaa cggtattgaa gttttgacaa
    12181 atgcaaaaat cgaagctttt actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa
    12241 aagaaatccc agcagatcta gtcattcaag ctgccggtgt gaaggcaaat acagaatggt
    12301 taaaagggat cgtcgactta gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa
    12361 cgaatcttcc agatgtgtat gcagtaggag atgctacact tgcttattct atcccagcac
    12421 gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa
    12481 aacatctttt tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat
    12541 taagtgtctt tgactatcat tttgcagcaa gcggattgaa tagctttaca gcagcaaaag
    12601 caggagtaga tgtacgagtg tcttattatg aagatacgtt acgaccaaaa tatgtaccag
    12661 aagaacaagg aaatccaaaa gtgtccatcc aattattctc ggatccaatg acccatcaat
    12721 tgctaggagg agcagtatta tctacctatg atgttacagg tcaaggaaat gtattggcac
    12781 tagcgatcca aaacaaactg actttagaag aactagcaga agctgacttc ttcttccagc
    12841 caggctttga ccgacaatgg agcatgttga atctagccgc tcaacaagcg ttaggagaag
    12901 aaccatttgt agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa agctgccttg
    12961 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggctggac
    13021 ttattaccgc agatgcaagc ttttgaacac cgtttatttt caaaaaaggg actgtgacac
    13081 gatttgtgta ccgaccccca aaagttagac caaaaaatct aacttttgga ggtcggtatt
    13141 ttttatggtt aaatatactt ttgaacttaa attaaagatc gttcatgatt atttggatgg
    13201 caaaggcgat tggaccgtaa aaatccagat caagagattg aagcggaaat gcttaaaatt
    13261 cgggagaagc acaaggatta tggctgttta cggatgacca atgaactaag aaaccgcggt
    13321 ttctctatca ataaaaagaa agttcagcgc ttgatcaaga agctaggaat aaaagtaacg
    13381 gcatacacgc gtaaatcacg tcgctacaat tcttatcgag gaaaagt
//
LOCUS       DACCCR010000073        12755 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCCR010000073 DACCCR010000000
VERSION     DACCCR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.080"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            555..755
                     /locus_tag="IVK93_002140"
     CDS             555..755
                     /locus_tag="IVK93_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6387418.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1323..1886
                     /locus_tag="IVK93_002141"
     CDS             1323..1886
                     /locus_tag="IVK93_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387419.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1947..2291)
                     /locus_tag="IVK93_002142"
     CDS             complement(1947..2291)
                     /locus_tag="IVK93_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387420.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2388..2633)
                     /locus_tag="IVK93_002143"
     CDS             complement(2388..2633)
                     /locus_tag="IVK93_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387421.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2703..2957)
                     /locus_tag="IVK93_002144"
     CDS             complement(2703..2957)
                     /locus_tag="IVK93_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387422.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3121..3444)
                     /locus_tag="IVK93_002145"
     CDS             complement(3121..3444)
                     /locus_tag="IVK93_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387423.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3631..3704
                     /locus_tag="IVK93_002146"
     tRNA            3631..3704
                     /locus_tag="IVK93_002146"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3665..3667,aa:Ile,seq:cat)
     gene            complement(4089..4586)
                     /locus_tag="IVK93_002147"
     CDS             complement(4089..4586)
                     /locus_tag="IVK93_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6387424.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4626..5435)
                     /locus_tag="IVK93_002148"
     CDS             complement(4626..5435)
                     /locus_tag="IVK93_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6387425.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5769..6626
                     /locus_tag="IVK93_002149"
     CDS             5769..6626
                     /locus_tag="IVK93_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6387426.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6619..7521
                     /locus_tag="IVK93_002150"
     CDS             6619..7521
                     /locus_tag="IVK93_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6387427.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7554..7904
                     /locus_tag="IVK93_002151"
     CDS             7554..7904
                     /locus_tag="IVK93_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6387428.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7938..8876
                     /locus_tag="IVK93_002152"
     CDS             7938..8876
                     /locus_tag="IVK93_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6387429.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8954..9847
                     /locus_tag="IVK93_002153"
     CDS             8954..9847
                     /locus_tag="IVK93_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6387430.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9885..10448
                     /gene="amaP"
                     /locus_tag="IVK93_002154"
     CDS             9885..10448
                     /gene="amaP"
                     /locus_tag="IVK93_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6387431.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10462..10650
                     /locus_tag="IVK93_002155"
     CDS             10462..10650
                     /locus_tag="IVK93_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6387432.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10663..11565
                     /locus_tag="IVK93_002156"
     CDS             10663..11565
                     /locus_tag="IVK93_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6387433.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11597..11839
                     /locus_tag="IVK93_002157"
     CDS             11597..11839
                     /locus_tag="IVK93_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6387434.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12095..12466)
                     /locus_tag="IVK93_002158"
     CDS             complement(12095..12466)
                     /locus_tag="IVK93_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387435.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4515 a         2133 c         2397 g         3710 t
ORIGIN      
        1 ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg taacacctgt
       61 cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa
      121 ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc tttctttaaa
      181 tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag tataccacca
      241 attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata aaagcaaata
      301 aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg attcgaagac
      361 aatagaagac aatgaatttt atatttcatt gcattatcct tgtgttatta gaaaaagcat
      421 gatatattaa atgagtaatc tattttgaaa cgtaatttga gcgatatatc tacatagata
      481 aaactccttt tgcctatcaa atatttattg caacgaaaaa acgtacattg atacgtatca
      541 ggaggaattt atttatgaat aacggtacag taaaatggtt caacgcagac aaaggttttg
      601 gctttatcac tggtgaagat ggaaatgatg tatttgcaca tttttcagct atccaaggtg
      661 acggcttcaa aactttagaa gaaggccaag cagtgtctta cgatgttgaa gatggtcaac
      721 gtggccctca agcaacaaac attgttaaag cttaataaaa attgactttt aaccgtcttt
      781 tcgaaagaaa agacggtttt tttgtacgaa aaaattcatt ggtcgttgaa tgggctgaat
      841 agtagtgtat aaaacaattt tgtggagcgt gaagctttta tggtagagga aaatacgaat
      901 aaagtgatgt tgataaaaag attcctatct tgaatatgaa tcgagatgtc ttgaaaatga
      961 atgagtttga ccaatcatta tcctatcgaa atttacagtt tcgttcgatt ggtctgtcaa
     1021 ttaatgaaaa agtaaaaaag aatatgttct aggaagaggg cggcacatct aaatttttaa
     1081 gtaacggaca gttcaaatat caagcattga aagaggctat cctactgttt gaaggaatac
     1141 gaatatataa aaacgacaaa gaggaaaaag tagttcatta aatcccttca agtgatgaaa
     1201 gaatgagatc taataaaacc tgatctttca tctgtttttt taataacgta ataatttttt
     1261 tattttttgt gctttcgccg ttattgtaga gtgatttagc attcttattt ttaaaaataa
     1321 ctgtgttata tattaaaaaa ggcataatag ttataagctt ttttaaagaa tatttagaaa
     1381 ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat tattttagtc tattttcagg
     1441 gggggaacct tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag
     1501 gttgttctga ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag
     1561 aggagttaaa atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg
     1621 gaatattagg cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa
     1681 gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag
     1741 acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag
     1801 ttggcgatga accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc
     1861 ctaaaactta cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga
     1921 ggagatagaa ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt
     1981 gctaaacatt ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct
     2041 acagtaggta aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac
     2101 tctggtgctg ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg
     2161 aataatctat aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag
     2221 ccatttcctc ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc
     2281 tttgaaatca atttttcatg ccataatcac atagaaatgt ctcctttata tatagattgt
     2341 taaccttaga cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg
     2401 cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata
     2461 tagattaatt caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat
     2521 aaaggaatac ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag
     2581 aaattcagac gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca
     2641 cctttcatta tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt
     2701 actcaatgtt ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc
     2761 agtcctgata attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact
     2821 ttccacataa agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc
     2881 caattcataa gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact
     2941 ctcaccaacg taatcatgta acggactcct caacgattca tataataaaa gataacatgt
     3001 tattgcatac atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa
     3061 tgaaaaagca gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat
     3121 ctatcgagaa aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac
     3181 gatcgcattc actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac
     3241 aaatactttt gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag
     3301 aagcaaaagg gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg
     3361 ataatttaat tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa
     3421 tggcaaaata atcactccta acattgagaa taaataaaag aataacattt tttctaaaat
     3481 aagcaaataa aaattaaagt aacaaaatag ttatgacaca agtaaaagaa ataagaataa
     3541 caatattatt ttacttatat tttgaaactt gtgagagttt gtcattcgtg ctatagttga
     3601 aaagtaacaa aaataaattt tatttatgat gtacctttag ctcagttggt tagagcagac
     3661 ggctcataac cgtccggtcg taggttcgag tcctacaagg tacataagag gttgtgaaaa
     3721 agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttact
     3781 tggctgttct tattactgca gatgcaagct tttgaatacc gtttaaggaa gaggttgtga
     3841 aatcagcgtt ttgacctgag tattagctta acaaacggaa aaatagttcc tcatattttg
     3901 ccgtttgtta agctaatacc gagggtcagc tgattgaaca ccatttaaga aagaggtcgt
     3961 gagcctgccg tttcactctg tgtcataaaa aagccaatct actttgatag aaaaagcacg
     4021 taggactaaa acagttctgc gtgctttttt tattgtgtgt taaatatcgt aaatgaagat
     4081 agatattttt atgattcgac gatagagacg ttgaaatctt tcaacagctc taaagcggat
     4141 tctccgtgtt ttctactgga gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa
     4201 gcactctgca ggcctatcag attagagagt acacacaagt ttgtttcaat cccgactact
     4261 tcgatctctt tttgttcttc tttgctttca aataaagttt cctggatttc agctaaagtt
     4321 tcaggtggaa tcgtaaagta atacttttta atcactcgtt cattttcttg tggagaaaga
     4381 agagggatca attgcaaatc ttctcgttct tcttctttgt ccttcagttc aactggtata
     4441 tcccgtgtga agagaatata ttcattattt tggcgtgctt tagctaatct ttgagcaata
     4501 cgttctgcca attgatctga ctctggaata taaaattcgc tgtttggatc aagaatatga
     4561 ttttgcatat caataataac taacattttc tttcctccta cattcatatc gtcattttat
     4621 tctttttaat agaagttttt taaaatataa gcaacaccag cttcttcatt cgtcagtgtt
     4681 acttggtttg caatttcttt tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg
     4741 ctgtaattca acatatcaat gtcgttttct tgatcgccaa aagccatgat gtcactcgat
     4801 tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag ctttagtgat acctgtaggc
     4861 gtgatttcta agaaatgcgg atcagagaat actgattgga taccatctgc ttgcagatct
     4921 ctttgtagct tctgtagttt ttcttcactc ttatctgtta atgctaattt aagatagcta
     4981 agagaaggat cttccaacat atttaaaatt tgatcaaaag aatggttctg taaattgaaa
     5041 tgttcataat aagggtcatc tttgactaga tttgattcat cgaatgtcag ctgatcatct
     5101 tgggtttctc ggctagcaat gatggatatt ttcgcttctt gagcatagaa gaaggctttt
     5161 ctcagtgtat cgctatctat ttttgaacgt ctgagaactt tcgccatttt tccatccttc
     5221 atttcagcaa tcagtgaacc attggaagaa atcaaatagc caccgattcc taattcggaa
     5281 aaaatatctt tagcagaaat gctgttcctt ccggtagcga cggttacgat tccgccattt
     5341 tcatcaaatt tccgaagtgc ttggatattt tcttctggta tctttccatt tcgatcaagc
     5401 aaggtaccgt ctaaatcaat cgctgctagt ttcattttat cttcctactt tctttttaca
     5461 tatctatttt cctgttacct taagattaaa tgagaattta aaaaaatgaa actaacttgc
     5521 tttgtcatta tgaatttttt caaaaaacat tcttaagaaa aaggtggcta tatatagtag
     5581 tttttggtca atacctttga ataaaaagcc atgaaaaacg aatagttagc gcttaccgat
     5641 gcgttttgtg ataaaaaaat tttaaaatat tcaatgaaaa caaagcatat attttgcttt
     5701 aaaaccaaat gaggaatatt ctagatgtag aacaggttag aaaaagatta gaaaaaggag
     5761 tgattttgat ggagaagaga tacggcgtag ttcctgtggt aactgcttca gacgaaaatt
     5821 atgctcctta cttgagcgtg atgatcgcaa ctgcattaga aaattgcaat aaaacaagac
     5881 gcataaaatt ttatgtgatt gacgatggtc tatcagaata tagtaaagaa ggattggaag
     5941 aaacggtaaa taaatattca agtaatgcaa gtatccaatt cctgacagtg gaaaaagata
     6001 tctacgaaga cttcctagtc agtgaccata tcacaactac cgcttactta agaatttctt
     6061 taccaaactt attagctaaa gaggattata agaaagtcct ttacttagat tcagatgtgt
     6121 tagtactaga cgatatcgta aaactatatg atgagccgtt aaatggaaaa acaatcggcg
     6181 ctattatcga tccaggccaa gtgaaagctt tggaaagatt aggaatcgat tccgatgact
     6241 tgtacttcaa ttcaggagtg atggtgattg acattgatca atggaataaa aaagaaatta
     6301 cagaaaaaac tatccattac ctcagtgaaa atggcgatcg aatcatctat cacgaccaag
     6361 atgctttgaa tgctgtactt tatgaagatt gggaacaact tcatccaaaa tggaacatgc
     6421 agacttcgct gatttttgaa cgtcatccag caccaaatga aaaatatgaa cgtcagtata
     6481 aagaaggaaa tgaaaaacct tcgatcgttc actttacagg acatgataaa ccttggaata
     6541 cgttgaaaga tcatccgtat accaatttat acttaaaaaa attagcacac agtactctaa
     6601 cgaaagtagg cgagatcaat gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca
     6661 aaattcgtac cacatttagc tgcattattt gtctcagtat tagataattg caacccgtct
     6721 aaatttgttc ggttctatgt gattgacgat gatatcgatt ttgaaagtaa acagctttta
     6781 cgcttctcag tgaaaaacgc acgaatgaac tcagatgtgg aattcttgaa aatcaataaa
     6841 gaattcttta caaatgtggt aatcagcgat cgtattccag aaacagctta ttatcgtatt
     6901 gcgattccgg aattgttccg cggcacagaa gttgaacgta tcttatatat ggattgcgac
     6961 atgatcgctt tacaagatat ctctaaatta tggcgtcttg acttcggtga ttcaatcgta
     7021 gcagctgttg aagacgctgg gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa
     7081 tcaatgcgtt atttcaactc aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa
     7141 aatattacgc aaaaagtatt agatttcatc gaacataatc cagaaaaatt acgattccat
     7201 gaccaagatg cattaaatgc tatcttacat gaccgttggt taccacttca tccgcgttgg
     7261 aatgcacaag gctatatcat ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt
     7321 gaatacgaag aaactcgcaa caatccttac attatccatt tttcaggaca tgtgaaaccg
     7381 tggagcaagg attttgaagg gccaacgaag aaatattacg aaaaatatgc agggatgacg
     7441 gctttccgtt gtgtagctaa attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa
     7501 acagatgtag ttgttaaata atagaagaca acaactgaag agggggagta ggaatggaaa
     7561 ttacaatgtc taaaaatgca gtagagacgt taattgaaaa ggttggggaa aatacaaaaa
     7621 tcgcactcgc gcttatcaat gattccgatc catttctaag ggacaaaggt gcttttgcaa
     7681 aaggcagctt ttttcaaatc attccttttg tttcggaatt tggagaatat gcgactaaaa
     7741 tcgagcatcc attattggac atctatacat ctaaattaga gcaaaattat ttcggtaaac
     7801 gattgaacat ggacttcaac aaacagcttg acagcttttc attagaaaat gaaatcgcag
     7861 tactcgatta taatatcaaa ttaaagaact gcttcttcag ctaatagttt cgagaaagtc
     7921 caaaaaggag gtatacattg agatactact ttgtggatga agaaaagaat gtaatagaaa
     7981 cagatgaaaa aaaatataat tggctcgtac ttgatagtgc aaagcccgaa gagattgagc
     8041 aagcaatcga agaattcgag cttcctgatg atattttcgt aggaaccgaa tatccggaag
     8101 aagtttctcg gatggagcgc ctttatgaga cgaaactaga gcatccattt tctttggtag
     8161 tgattaatct agatcagtca gataatcgta tcgaaaaaaa attaacgcca atatcttttg
     8221 tactttccga taatctcttg attacttgta tcgatagaaa aaccgaattt gtggatcggc
     8281 tgctgacaaa gcatggaaat catttaaact cgtttgaaaa gatcatcacg tatacattat
     8341 tagatattta cacccattac gtgaaagaac taagagagat gaaaaagcga attgacgctt
     8401 tggataaagc agctcgtaaa acaacggaaa atgaagaact gtataaacag gcagatcttg
     8461 aaagagacat cgtatatatc gatcatacct taagagatca acgggaaaca atggatcaat
     8521 tatggaattc tgaaactttc atcaaacgtt tagacgatga acagctactc tatgatgtcc
     8581 atttgagaca gcgacaaaca gaaaaaatga tcgagatcta tcgagattta ttagagagta
     8641 ttggtgatct attcaacggg atgatggaca ataatctaaa tcatatcatg aaatatctag
     8701 attcagctgc attaatcatt tccgttccgg cgttgttcgc agggatttgg ggaatgaata
     8761 caggaggatt accgggtaaa agttctgctc taggctttat actcgtagtc tttggctctt
     8821 tgatagcagc agtagtttta ggttaccatt tataccggaa agattataca aattaaagaa
     8881 tctatttaaa acaggtatca aacaaagaaa tttgttttgg caatacaatt taaaaactag
     8941 gaggaataca gcaatgacag aaccaaaatt aatcgacccg cgaaacttgt accatacgga
     9001 gaaatttcct aaacaagatc aggatacacc agctttacag cagaatatga ttcctgttcc
     9061 tgattgtgga gaagaatcct atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat
     9121 cactggtgga gattcaggga tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc
     9181 agatatcgct cttcaattct tcccaggaga agaaaaagat gcagaggaag tagcaaaata
     9241 cgtgaaagtt gcaggacgta agattgtctt actacctttt gatcttcgtg acgaacaagc
     9301 acctaaagaa atcatagata aagccgtgag tgaacttggc gggctagata cgatggtact
     9361 aaacgcagga cagcaaattt cttgtcagtc tattgctgag ttaccacttg agcaagtcaa
     9421 agacacattt atgataaaca tcatcgctat gtttgcttta gtgaaagaag ctgttccgca
     9481 tatcccagca ggaggagcaa tcgtaacaac aacttctgtt caagcattca atccaagcga
     9541 acatttatta gattacgcag caacaaaagc ttcaatcgct aactttacga tcggtttagc
     9601 aaaacagctt gcacctaaag gaatccgtgt aaatggtgta gcacctggtc caatctggac
     9661 accattacaa ctagacgaag gacaaccttc tgattcgatc cctgaatttg gtcagcaaag
     9721 tttactagaa cgtgcaggtc agccaggaga attagcacca gtttatgttt tcttagcatc
     9781 gaataaagca agttatgtta cagcccaaat ttacggagta actggtggag aagcgatcaa
     9841 cttataagaa gttgatcgtt tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa
     9901 aaacgatcgg agtgatcgga acattacttt tgctatctgt gcttttgttt gcgatactat
     9961 taagtgcaac ctatccattg ccttttcagc tagagaagtt tcgattcttt tccatcacta
    10021 attcatacgt gcaacaatat gtattttggc ttgccgttgc atttgctatt ttgacaatta
    10081 tcacgttact tgttttcctg ttttatccaa aaacaatcgg aaactttcta ctgaaacatg
    10141 gtgatgggga gttgacgttg gataaaaaag caattgaagg aatggttcgt tctcacttgc
    10201 atgaagatga attcatccat tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg
    10261 ccgtcaatgt aaaaggggag ttgaaacgaa cttcagcact cattggtaaa acaggaacat
    10321 tgatgaaaga aatcgaaaat gaagtacaac acatattggg aacaaaagag ccagtgaaag
    10381 tatcggtgaa atacacaggc tttgaagaac cagctaaacg aagcaatgac cgcaaacgag
    10441 tagaataggg ggggaccaaa aatgaaagaa ctattttcga catacaagct ccctatcatc
    10501 tttggggtac ttggattgat attagccgtt ttatggatca ccgtgggctt tttcaagacc
    10561 ttattgatct tgatcatggc agcgatcggc gtcgctatcg gtttttatct gaaacaaacg
    10621 aaaatcttag atcactattt taaatcttag gaggaaacat ccatgaataa caataacaaa
    10681 aataataaca ataaaatgaa caaccaaaaa ccagtgtctg cacatccaga cccaacgaca
    10741 ccaaaccaat caaatggtgt gaacgatgaa aagaacaaaa cagcaccagt atctgcacat
    10801 ccagatccag tagtccctaa cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa
    10861 aagcatgcac ctcatcatcc aggaactcca gaatctaaag ttccagaaca acatgatgca
    10921 atcgatcctt ctaaacctgt ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa
    10981 gaaaaactta gttctgacca cacaaacgga caaggaaaac ttggttctga tcatacaaat
    11041 aaccaaggaa agcatgattc tgaacaggaa gattcaatgc gcggtgagct gaattatgaa
    11101 gataaagttg tgcaaaaaat catcggaatc gcaattgaac aagtagacgg tttattatct
    11161 gcaaacggtg gtttcttctc taatgtggca ggaaaacttg tgaatacaga taatgtaacg
    11221 gctgggatcg aaacagaagt cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg
    11281 gaatatggca aagatatcga aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa
    11341 gtaaaaaata tgacacattt agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa
    11401 acaaaagaag aatttgaaaa agatcaagaa acggtgcaag acaaagtaac tgacgcagcg
    11461 aaaaaaaccg gtgaatttgc ttcgaatcaa acagataaag taaaatcagc agctggaagc
    11521 ggcgcacaaa aagtaaaaga aaatacagaa ccacgggttc agtaataagg aagtaaaaca
    11581 ttaggaggaa cgaaacatgg gatttatttg gtcacttatc gtcggaggaa ttatcggagc
    11641 aattgcagga gcaatcacga ataaaggttc ttcgatgggg attattgcaa atgtcgtagc
    11701 aggtttagtt ggatcagcac ttggtcaagc attattaggt tcttggggac caagtattgc
    11761 aggaatggca atcgtgccat cattgattgg tgccatcatt ttagtactaa tcgtatcatt
    11821 ctttgctcga aaactgtaaa aagactttta aaaagaggtc gtgaaaaagc tgttcttcat
    11881 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcatttg gttgggcata
    11941 ttatcgaaga tgcaagcttt tgaacactgt ttatacggaa taagaggctg tgacaagact
    12001 tttgtcacag cctcttattt atgcgggcaa attcccaatt agatttttaa aagtttttcc
    12061 ttaaatgtgt ctttattgat acagagaagc ttctttaata aaacaatgcc tccctaaatt
    12121 ttcttgagat aggaaaggtg aacatacaaa ataatacgac cactttgaaa acccagatga
    12181 tccacaaatg gatcgtatga tactgatgaa gtaaaaagca atggatcaga aactcaaaga
    12241 gattgaaaaa gacaaggaag aaataatatc tcttttttac tcttgtctgt tttagccaat
    12301 aatagctcgt aaagtaatct agacaaacaa atagaagtaa acaaagtttc gtaagagcag
    12361 caatcgtatc atgttgctct gctagacgat tgacgagcag gaaaaatgtt ggtaatggaa
    12421 tatagatcac gagtaatgaa agaaaaaagt aaacaataaa tggcatacta ttcctcctaa
    12481 ttgtgtgagt tcgccctcat tttaaaataa cgatatttgt ttgtgaagct ttttcgtgtc
    12541 gtaacaaatg ttatctaatg tatttttaaa taaataatca ttattatcaa aataatgtca
    12601 gactgtagac aaagtccatt ttatggatga tgtctacagt cttttctttt ataatagatt
    12661 tatgaaaacg aatgcaagga gtgatagcta tgatgtctaa aaacaatacg aatggtagaa
    12721 atcaatttgc gatgcttacg attgatgatc tagtt
//
LOCUS       DACCCR010000074        12735 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCCR010000074 DACCCR010000000
VERSION     DACCCR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.081"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            5..304
                     /locus_tag="IVK93_002159"
     CDS             5..304
                     /locus_tag="IVK93_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6387436.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            377..1738
                     /locus_tag="IVK93_002160"
     CDS             377..1738
                     /locus_tag="IVK93_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6387437.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1760..2020
                     /locus_tag="IVK93_002161"
     CDS             1760..2020
                     /locus_tag="IVK93_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387438.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2034..2786
                     /locus_tag="IVK93_002162"
     CDS             2034..2786
                     /locus_tag="IVK93_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP6387439.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2880..3485)
                     /locus_tag="IVK93_002163"
     CDS             complement(2880..3485)
                     /locus_tag="IVK93_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6387440.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3538..4512)
                     /locus_tag="IVK93_002164"
     CDS             complement(3538..4512)
                     /locus_tag="IVK93_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP6387441.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4890..5669)
                     /locus_tag="IVK93_002165"
     CDS             complement(4890..5669)
                     /locus_tag="IVK93_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6387442.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5736..7007)
                     /gene="celB"
                     /locus_tag="IVK93_002166"
     CDS             complement(5736..7007)
                     /gene="celB"
                     /locus_tag="IVK93_002166"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6387443.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7160..8557
                     /locus_tag="IVK93_002167"
     CDS             7160..8557
                     /locus_tag="IVK93_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6387444.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8638..9078
                     /locus_tag="IVK93_002168"
     CDS             8638..9078
                     /locus_tag="IVK93_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6387445.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9226..9552
                     /locus_tag="IVK93_002169"
     CDS             9226..9552
                     /locus_tag="IVK93_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6387446.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9553..10302
                     /locus_tag="IVK93_002170"
     CDS             9553..10302
                     /locus_tag="IVK93_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387447.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10382..10837)
                     /locus_tag="IVK93_002171"
     CDS             complement(10382..10837)
                     /locus_tag="IVK93_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6387448.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11264..11539)
                     /locus_tag="IVK93_002172"
     CDS             complement(11264..11539)
                     /locus_tag="IVK93_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387449.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11833..12648
                     /gene="thiD"
                     /locus_tag="IVK93_002173"
     CDS             11833..12648
                     /gene="thiD"
                     /locus_tag="IVK93_002173"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP6387450.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVIIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4077 a         2187 c         2598 g         3873 t
ORIGIN      
        1 aaaaatgaaa aatcggttaa ttgtcgcatg tggaagtgga gtagcaacga gtcagacaat
       61 cgctagtaag atccaaagta tgttagaaga tgacggcatc gcatttcctg tagaagcagt
      121 tgattacaaa tcgattcaaa atgagcttct aactgctgga atctatgtgt atgtcgcaca
      181 acctgatgag gaagtacttg aacaagcaaa agatttgggt attgaagtat ttccaggaat
      241 tccattttta actggaatgg gtgtagaacc tatttatgac agtatcaaag agttagtaca
      301 gtaaaggagg attgttggat aaaaaagttg gttcatagtt ttatttacta tatttagaaa
      361 gaaagaaggt ttatttatgg aagttttcca aaccattatc aattatgttt tgaacttagg
      421 atcagccatt tttgttcccc tgattatttt gatacttgga ttgttggcag ggatgaaatt
      481 aaaaaaagca ttcatgtctg ctttgacttt agggattgct tttagcggaa tgtctatggt
      541 cattggctat atgtctaacg cagtaagtcc agcatcagaa gcattggcta aaaatacggg
      601 aatcagtttg cctgcacttg atcttgggtg gacaggtgca gcaagcatca catggtcgtg
      661 gagttatgct tttatatttt ttgcagtaac gattggggtc aattttgtac tgttactttt
      721 taattggacg aagacgttaa atgttgatat gtggaatgtt tgggggaaag ctttaactgc
      781 ttatttagtt tattatgttt ccggtagttt ggctgcaggc tttcttactg caatggtcca
      841 agttatattg gaattaaagc ttggtgatat gtttcaaaaa catatacaag atctcacagg
      901 gattccatta gtaacagtga ctcattttat gacatctgcc gctgtcttgt tgctgccttt
      961 caatatgatc atggataaaa ttcctgcttt aaataaacga gcggatacga atgcattgaa
     1021 gaagaaaatt gggatcttct ctgaaaactc tgtaatggga ttcattattg gattggggct
     1081 tggctttgct gctgcgtatg gcgtttcagg ttctcttaat ctagcgatcc aaacagctac
     1141 agcattaagt ttgtttccta tgatctctaa aatgttcatg caatctttgt ctcctctagc
     1201 agacgctatg tcagaattta tgaaaaagcg gtttaaggat cgtgaagtat atatcggatt
     1261 ggactggccg atattagctg gtcgttcaga aatttgggtt actgctattt tacttgttcc
     1321 tgtgtttatt ggttatgcca ttattttgcc aggaaaccaa gtattgccat tagcagggat
     1381 tatcaactat tcgattgcgg tcggcggatt acttttaaca ggaggaaact tggctcgtat
     1441 gctgatcttg ggaatcatca ctatgcctat ttatttgtac ggggccactt accttgcgcc
     1501 aatactcagt gatttagcag aaaaaagcgg agcagtcgag ggaatcaaga aaggacagtt
     1561 gatcacatgg tcatccatag aagggccgga gttcagggtt ttatttgcgg aggcatttaa
     1621 taggaatatt ctcgctattg taggatgcgt gatttttcta ggactgtttg ttctacttta
     1681 taaatatatg ataaaagcga aagtaccgag tgaacgttat gctgaactac aaaaatagca
     1741 ttgaaatggt gggaaagtaa tgaaaagaga agagacagat aaaataaaat ggacagtcgc
     1801 tctttgtggt acacttctgc tatttctata tggactcttt actcagaata tcatcatcaa
     1861 tctattggtc atttttttcg cactagtgat ctataaatat ggaaatcacg ttttattcag
     1921 agagtacgat gaaaaaagaa aacggaaaat agaagaatca atgaaaataa aagaagctac
     1981 caaagaaata ttgagagaaa aaagttttat aaaaagataa tggaggggtc attatgtcag
     2041 atggacgtat gttatttgag agagaacgac aggacatggc acgtatcgta agagtgattt
     2101 tcgaaaggaa aaacacaaat gtcgcaggag ggaatttttc ctttaaaacc actgatgatc
     2161 aagggaaaga ctacatcatc atgacgccga ccatgatgtc agaagcctat ctaggagaga
     2221 tttctcctgc acaggttcta gtagtagaac caagaacgcg tagaatcgtt gacggagtcg
     2281 gtcatttaac tcgtgaaatc aatatgcatg aagcgatgta tgagactaat ccagctatca
     2341 aagctgtcct acatgctcat gcgcctaatg caatgttctg ggcgactact gggttgccta
     2401 tgcctaattt gacagaagcc acagctaaag taaaagagat tcctgtcatg gactttgagc
     2461 ctaattgctc tgaagaactt gctgaattga caagcgacta tgtaaaaaaa cagcatctcg
     2521 aattaccgca tatgttgctg ctcaactctc atggcgtatt gatcaacaca ggtggagaat
     2581 caggaatcga agctatccat aaagcactgc aaatccttga tacagttgaa tggaatgcag
     2641 aaattgccta caagcagacg atttttcaag cattagggat tactgacggt ttttattcaa
     2701 aaggtgagaa aatcggggga ttggaagagt tgaaacataa aacggctatt tataataaat
     2761 ctgttattgc agctggtgga gactaaccta tctgacacaa aaaactacga ctttcacaaa
     2821 atctgatcca gatcgatatc tgcttcaggt tgtattatgt aagtcgtagt tcttttcttt
     2881 tatttagtta agaggtggtc tgtccattcc ctcgcttgct tgatttcgtc catcgtcaac
     2941 tgatgacctg ttacagttag ctgaatagtc acatttgctt ggcgtgtttc gaattgatcg
     3001 gccaattctt taaaggaagc aggggaaacg attggatcgt tatcaccata agaaatccag
     3061 acggttttat cctctaatgt ggaacggctt tgatgaacac ctagagacat cggatggaaa
     3121 aacaaccctt tatttagctc ggtctcacgc tccaataata aatgagcagc gatgtttgcg
     3181 ccatttgaat agccgacaag cagccagtca cttaacggta tcagttcttt ttcgctgatt
     3241 tctttgattt tttcatggag tttatctgtt tctttttcca aactgtccag gtcgaattga
     3301 ttcaatccat tacgcttgaa aaaacgattc atcccatctt cttggaccgt gccacgaaaa
     3361 gaaagcaatg tactgtttgg tgcaagaaag tgagcgatat ccagtaaaga atgttcatcg
     3421 ccacctgtgc catgaagtaa gatcaaatgc ttgccacttg ttcctttttc aaaaatgtag
     3481 gtcattttgt tccctctttt ctgtttctat agatgcaaat gaagtagcat gaagagatta
     3541 ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg acataagctt caatggcttc
     3601 acgacgttct tctaagaatg gaggaagaga tagtttttct cctgctgatt ctgctggttc
     3661 atcttcaagg aatccaggac catcagttgc taattcaaac agtacgtgat gagcggctag
     3721 gaagtactca gattggaaat agaaacgatc gacgaaacca gaatttggca attcaatctt
     3781 attgattact tgaatccagt attccaatgc ttctttgtct gctacacgca atgctaagtg
     3841 atggatattt ccaaatcctt cgattgcttt tggcaagtct gtccgatgtt caataatgat
     3901 gcttgcacca tttccccctt caccgatttc aaataagtag aaatctcctg aatggtctgt
     3961 ttctttgaat cctaagattt tttcaagaat caagcggata tgctctaatt gactgacacg
     4021 gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc ggtacattgc tgtttttcca
     4081 aggaatgcca gctgctacac cttgattgtt ttcgtcagag atcaattgat aatactgatt
     4141 atcgaaatct tcaaattcga tatatttttt gccgaaacgt tcattgattg ctgaatgagt
     4201 aacgtcatat ttttcaaaac gttccaacca aaagtctaat gctgcgtcac ttggcacacg
     4261 gaaagaagca cgagagatcg tatctgttcc tttttttcct tttggctgat ttggaaaatc
     4321 aaagaaagtc atatctgttc ctggcgcacc agtatcgtct gtaaagtaaa gatggtaagt
     4381 ttcgatgtca tcttggttaa ctgttttttt gattaagcgc attcctaaaa tatctgtaaa
     4441 aaagcggtag atttttgtag cgctagatgt cattgctgtg acgtggtgaa ttcctctgat
     4501 ttgtgtatcc atgataaatt tcctcctgtt aacttacaaa aagtaagtag ttgatttaga
     4561 catagtatag agggtgcatc tctattttgc aattaatttg tcttctaaag tatcaggaag
     4621 gaatcaagaa tggaaaagag gagggacgga tcaataaaat aaaaagactt acgactagct
     4681 gtatcgattt caaaaaaact agctttaaaa agctaacttt agaatcatta cgggtggtca
     4741 taagtctttt tatgctgagt tattttttca tttttgagct aatgcttagg atagaataga
     4801 atgttgattt cacgatctct tcttgttact tgaaactgct taccactgtc acgatctctt
     4861 ggtaaacggc tttttcacaa cctctttggt taagttgaac gcacatccag cacagaatag
     4921 ttccgaacat caaatcggtt tcggctggaa gaatattcaa tcggctgtcc atctttgaga
     4981 tagacgactt gctgaacttc cagtacagga tcgttttctg cacagttcaa atattgctga
     5041 tcatattcat cacttttatc tgcagaaata gcacgataag cccctgcaaa atggacattc
     5101 aattcttgat gaagatgatc atagatagag gctaacaaat gttctttttt taaacccggc
     5161 acaagatgga ctggcataaa ggtatgctct aaaacatatg gctcgccatc taagatcctt
     5221 aaccggtgaa tattgtagac aggttgctcg gcatcgatca tcaatcgttc ttggatatat
     5281 tgattaggga attcaacatc aaaatgaatg atttgactct tcaaagaacg atgtctgcgt
     5341 ttcatttgga gactcaaacc ttcatactcg ttagcgggcg atgtgtcttt ttgcataaat
     5401 ggatgattca agattttcgt tcctgatcct cgctgcgaat agacaagtcc ttccattgct
     5461 aagatattga tggctttttt gattgtcatc cggctcgcac caaattcttt taccaagtcg
     5521 acttgattag gcaaaaaact atcgagagga tactcttttg aacggatacg ttctcttaaa
     5581 atatttgcga tttcttcgta ttttggcacg ttattcacct cttctagagt ataacgaaag
     5641 attatgcttc cgacaactcg aagtagcatc tggaacattg tttattcgaa aaaagaggtc
     5701 atgacaagtt tttgtcacaa ccccttaatg atctattaag caatatctaa atctggttgt
     5761 ttatctagta ctttcaggaa tgggtaccag ataaaggcaa cgatgacaaa gtcgatgact
     5821 tgcagcatac caccaagcca tgaatttgtt gctaagacac cgcttgcaat gattggcaca
     5881 gtccatggaa cagagacgcc agttggtgca gggacaagtc cagcagccat taccaaataa
     5941 ttgaacgtcg ttacgattag aggtgccaca acccaaggaa taaggattgt tgcattcaat
     6001 acgatcggta gaccgaagat cgctggttca tttacattga aaataccagg tgctaaagct
     6061 aatcgtccaa catcacgata ctgtttcttt ttaagaacaa aggccatcaa taaaacgaca
     6121 gctaaagtca ttccagagcc accaatacca acagtgaagg tttccatgaa tggttttgtg
     6181 atgatgtgtg gtaattcttt tccatgttga taagcttcta agttatcaag catcagtgta
     6241 ttccaaatcg ggtccattac agagttgacg atgatttgtc cgtgaagtcc gaagaaccat
     6301 aagaattgga caaagaacaa agcaatcaat gttgcaggta aactacttcc caaaccagtt
     6361 aatggttttt ggatcacttc ataaacgacg tcatgtaagt tagcattgaa aataccagtc
     6421 atcacagcat tgattactaa gaaaatagtt agtgtcacta cagcagggat caaagcggca
     6481 aatgattttg ttactgcagg cggaactcca tcaggcattt tgatctgcca gccgcgtttc
     6541 gtgattctgc agtagatttc tccagccaag aaagcagcga tcattccaat gaacattcct
     6601 tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat cttcaccgcc agcggttttc
     6661 attgcaaaag gcgtaagaat caagaagctg gcaaaagaca cggcaccacc gaaaatacct
     6721 tcaacgtcat aagattttga taaataatag ccgataccaa aagtaacaaa aaccgacatg
     6781 atgctcattg ttgcattttg cccattaccg aataaattcg ataaagttcc ctttgttgcg
     6841 tcactgaaaa aaggcaagtt attgataacg acaacgatcg aaccgaacat cgttaaaggg
     6901 aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac gattttgccc taataaatta
     6961 gctaagggca tgattttctc agaaagcttg tccataaaac cgttcatttt tattcacctc
     7021 aaatttattt gcaatcgatt acatacaaag tataacgcca aaaaatttaa atgtctagtc
     7081 attttcataa aaagtattga tttatttttt agggttcgtt atgattacgg tatcaagaaa
     7141 aaagagagga agtaaaaaaa tgaaaaccta cacattccca gaaaattttt ggtggggttc
     7201 tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat gatggtaaag gagaaaatat
     7261 ttgggatcat tggtatgaat tagagcctga aaaattcttc gatcaagtag gacctgaaaa
     7321 aacttcgcaa gtttacacaa aatacaaaga agatgttcag ttaatgaaag aaactggcca
     7381 taacacattt cgtacatcta tccaatggag tcatttgatt ccagaaggga caggtgaaat
     7441 caatcaaaaa gctgtttctt tttatcgaga ttattttact gaattgctgg ataatggtat
     7501 tgaacctttt gtgaaccttt accatttcga tatgccacta ccactgcaag aaaaaggcgg
     7561 atggttatca agagaaacgg tcgacgctta tgtttcttat gcagcagcgt gctttgaact
     7621 gtttggagat ctggtcaaaa aatggtttac tcataatgaa ccaatcgtac ctgtagaagg
     7681 aggctacttg tatcaattcc actatccaaa tcatatcaat atgaaagaag cggtccaagt
     7741 aggctatcat gagaatctag caagcgcaaa ggtaatcaaa gcctaccatg aaggcggaca
     7801 tgatggtgag atcggaatca tcttgaatct gacaccaagc tatccgagag atgaaacgaa
     7861 cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc ttcaaccgat cattccttga
     7921 ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa attgtcaaag aattggctat
     7981 aatgccagag atccatgaag gagacctaga actcatccgt gaaaacacga ttgacctgct
     8041 aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa gaaactccaa tcaatcacga
     8101 acatagcccc atgccagatg attattttga taattacgac atgcccaata aaaaaatgaa
     8161 tccatatcgt ggatgggaaa tttacgaaaa aggaatctat gatattttaa cgaatacaag
     8221 agaaaactat ggtaatatca aatgctatat ttctgaaaat gggatggggg tcgaaggaga
     8281 agaacgattt gttaacagcg aaggaattat tgaagatgat taccggatcg agtttgttac
     8341 ggatcatttg aaatacgtcc atcaagcaat ccaagaagga accaattgtg tgggttacca
     8401 catgtggaca tgtatggaca actggtcttg gacaaatgct tataagaatc gctatggctt
     8461 tatcgctgtt gatttggcac aagacggtaa acggacgatc aaaaaatcag gtcgctggtt
     8521 taaaacggtc tctgaaaaaa acggatttga tgcctgatat tttttgataa aacgttcaca
     8581 aaatgtttgt tctaacactg taatcgtgct acactaacag tgaaggaggc gtttacaatg
     8641 gtagatactt atcgtaatat tttagtagca attgatggtt ctgaaaaggc agaaaaagca
     8701 tttcttgagg caatcacgat tgccaaacga aatcaggcaa cgttgcacat cctgtatgtc
     8761 aatgaagtaa caggcaatta ctttggtgat tttgcatttg ttacaacaag ccttcaggaa
     8821 gaattggatg aggttgcgga aaaccagatg aaagaacatc gaaacttggc aattgaaaaa
     8881 ggcctgacag atatcgagac atatgttctt tacggttatc caaagacact gattgctaat
     8941 ttccatgaat cgaaagaacc aatcgatctg attgtcatgg gggcgacagg tctgaatgcg
     9001 gtagaacgtg cactagtagg ttccactact tcctatgtag tcaaccacgc atcatgtaac
     9061 gtcatggttg taaaataaat aaattaaggg aacagaaagc aaaaaagcat aacgcttctt
     9121 ttgctttctg ttccttttat ttttttgtaa aaaattactt tacctgatga agtaatcatg
     9181 ctattctaat tacaacgaca gatggagtaa tatggaggaa agaaaatgat ttcaagtgat
     9241 ggaatacgcg gatacaatga tgcgattatt ttatcgatcc ttaacaaaaa cgattcttat
     9301 ggatatgaaa tttctaaaac gattcgtgag aggacaaatg attcctatgt gatcaaagag
     9361 acaacacttt actccgtttt tgcccgttta gaaaagaatc aactgattgt ttcgtatcct
     9421 ggagaagtga ctcatggaaa aaagcggaca tattataaaa tcactgaaat agggcgtaat
     9481 cttttagaag aaaaaaagca agagtggctg acgacgaaag acgtagtcga tcaatttttg
     9541 gagggaaatt aaatggatac aatccgtaat tatttagaca gtctttttat cggagtacct
     9601 caaagtaccg agatagataa gctgaaaact gatttgttgg caaatatgga agaccattat
     9661 catgaattga tgggagaagg aaaaaatgaa caggaggcca tcggtacagt aatttcgaca
     9721 tttggttcaa tcgatgaatt attagaagaa ctagatgtcg aaaaaaaaca tcaagctgac
     9781 gagacggaaa caaacacagc ttctatttat ttaagcgaag cagaaaacta ctggaaagaa
     9841 tacagagcgg caagtctcca agtggcatca ggtgttctat tcattagctt atcttttgca
     9901 agttttcttt tcttttgttc agcaggatat gtgttcatgg gtatttcttg tctgattttc
     9961 ggtatagcat tagctgtcgg tttttttatt gcttctggta tgaaaatcac acgtttaaat
    10021 cactttttgc accatcggaa aatacctgaa aaagtattag cagaagcaaa agaaaaagaa
    10081 gaagagtatc agcgaagttt tggcttctca ctgattgcag gaattggatt atgtattttt
    10141 tcacttttcc cacttttggc ttctttaatg tggtatatgg atggtagtat cggtgcctct
    10201 atattttttg ttacggtagg aacgggtgtc ttcttgatca tttatggcag tctagtccgt
    10261 cattcttatc gtcaattcac tcaaagcgct tattactggt gaatgtattc agatttgtaa
    10321 aaatattcta tttatccggc gtagaaaaaa agactgtgac acgcttttgt cacagtcttt
    10381 tttatatata ataatttaaa aattcatcat ctaaatccgt agaattttct agattgctta
    10441 cccatgtaaa atcgttatag acagatttca gttgacttct gcgccattgt acagggataa
    10501 aaagacgcat gctatcttca ttgaatccga ctaataattt ctttccgtca gtaatgatgg
    10561 gacgttgtaa caaagtaaaa tgcgagacga tcagatcgac aagctctgag gtcgtacatt
    10621 ggtcgatttg gtctttcaaa tttttataat caatcgagcg tttagctaat agatcgtcaa
    10681 atccgttttc tgttaatgac aataaataaa atatctcttt ccttgttaaa ggctgtttag
    10741 gtgtgaaaat tcgttctctg taaggcattt gatggtcttt cagccagttt gccatcagat
    10801 ggtaactatg tgtgcgagaa gaatagatcg taagcataaa aaaactcctt aaatagtcca
    10861 ttaatttgtt agatctgttg agataaataa taggtaagga gctgtgcata agcattgatc
    10921 aaaacaaccc aaacatcatt agaaataact ttctaaagtc ttaaacttgc gacgagaaac
    10981 aactagacta atagaaaaaa tgtcttagcc atttggcttc ctgtttgttt ttttgcttat
    11041 gctgcagctc ctcgggaaag ggttctaaca atttgaagaa ggttgatgat tgatctagtt
    11101 taaattgtgt gaaatttgaa atctggtgtt tttactagat gaatcaccta acaactatga
    11161 ttataactta tagaacttct gttttgcatt cggttttgtt atagttgtta taaagtttta
    11221 ttttattaca gaacattttt aattaataaa gaacagttag aagttaaaat tgctgattca
    11281 catagagttt atgacttctt ggaaattctt tttcaatcag ttttttcatc tgtttatgaa
    11341 attctttttt ttcagtaaac ataaaggttt gggtatcgta accttcttca tgtaggaaat
    11401 agattattcc tttttctgtg ttttgcgtat aagcttttat actcctgtct tttttgaaag
    11461 aacagatcgt atagccatct atttggtctg gtagtcgttt gattttcttc aaaatgactt
    11521 ttttatcctc aagtttcatt tttttcacct gactttattt ttttcatatg gaaatcatag
    11581 cgcctttggt tatatgaaaa caagaaggat actgaaatat atataaatca aatagtggtt
    11641 atttattatt agaatagcga atacaaataa acgaccacta ttttctgatt tattgcaaat
    11701 gttttttcaa ataaatttga ttaccaagaa aaggtagtgg tatatttact gagggatact
    11761 ttgaaaaaca tatgatagaa acagtaacag tattatgaaa aatcttttta taaaaatgga
    11821 ggaatcgatt tgatggaaga tcagtatgta caagcaatga cgatcgcagg atcagatagc
    11881 gatggaagcg cagggatgca agcagatatg catacattct ttacaagaaa tgtttacggt
    11941 gtttctgtca tgaccgcatg tgttgcaggt aattcttacg gaatcggagc aagtgtgaca
    12001 ttgccaacag attttatcga caaagaattt gaactgattg cgaaggactt tcaaatacgt
    12061 gcagctaaaa caggtatgct agcagattca aagttgattg agacagttgt aaaaaattac
    12121 caaaaatatg attttggacc attagtcgtg gatcccgtaa tcgtaacgaa acatggaaat
    12181 cttttactag aagaatctgc tttgcaaagc ttgaaagaaa agcttgtccc tttggccgaa
    12241 gtactgacac ctaattttta tgaagcagaa aagctgactg gacggacatt agcagctgaa
    12301 gaagattttg tgcaagcagc aaaagaactg caagatatgg gtgcaaaaaa cgttataatc
    12361 aaaggacatc atgctaaaaa tgcgcagcaa gaaacagtca atgactacgt attgttcgaa
    12421 tcaggaaaat cattttggtt aaatgcacca ttttatccta ccgaccggat caacggaact
    12481 ggcgattcgc tatctgcatg tattgcagct gagttagcga aaggaaaaac agttgaacaa
    12541 gcgatccgat tggcaaaaga ctatgtaaac cgtgcaattc gtgaagaaat aaatgtaggg
    12601 cacaaatatg gccccatcaa tcattgggca gctggtgaat tagagtaaaa gaaagaagtt
    12661 gtgaaaaagt tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
    12721 ttttaacttg gttgg
//
LOCUS       DACCCR010000075        12458 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCCR010000075 DACCCR010000000
VERSION     DACCCR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.082"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            59..751
                     /locus_tag="IVK93_002174"
     CDS             59..751
                     /locus_tag="IVK93_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP6387451.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            901..1101
                     /locus_tag="IVK93_002175"
     CDS             901..1101
                     /locus_tag="IVK93_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6387452.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(1355..3034)
                     /locus_tag="IVK93_002176"
     CDS             complement(1355..3034)
                     /locus_tag="IVK93_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6387453.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(3036..3248)
                     /locus_tag="IVK93_002177"
     CDS             complement(3036..3248)
                     /locus_tag="IVK93_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP6387454.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            3641..5371
                     /gene="efrA"
                     /locus_tag="IVK93_002178"
     CDS             3641..5371
                     /gene="efrA"
                     /locus_tag="IVK93_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP6387455.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            5368..7128
                     /gene="efrB"
                     /locus_tag="IVK93_002179"
     CDS             5368..7128
                     /gene="efrB"
                     /locus_tag="IVK93_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP6387456.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            7223..7420
                     /locus_tag="IVK93_002180"
     CDS             7223..7420
                     /locus_tag="IVK93_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387457.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            7573..8058
                     /locus_tag="IVK93_002181"
     CDS             7573..8058
                     /locus_tag="IVK93_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP6387458.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(8162..8755)
                     /gene="clpP"
                     /locus_tag="IVK93_002182"
     CDS             complement(8162..8755)
                     /gene="clpP"
                     /locus_tag="IVK93_002182"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP6387459.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            8935..9462
                     /locus_tag="IVK93_002183"
     CDS             8935..9462
                     /locus_tag="IVK93_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP6387460.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            9553..10290
                     /locus_tag="IVK93_002184"
     CDS             9553..10290
                     /locus_tag="IVK93_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6387461.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            10294..11211
                     /gene="miaA"
                     /locus_tag="IVK93_002185"
     CDS             10294..11211
                     /gene="miaA"
                     /locus_tag="IVK93_002185"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP6387462.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            11213..12442
                     /gene="hflX"
                     /locus_tag="IVK93_002186"
     CDS             11213..12442
                     /gene="hflX"
                     /locus_tag="IVK93_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP6387463.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         3983 a         2173 c         2702 g         3600 t
ORIGIN      
        1 ataaaaatcc atctcttttc gtatcttatt ttagataaga tagaaaggag atgttttttt
       61 gggaaaacga ttgatcaaag aagtacctac tagctcttta ccaagggaac gtatggaaat
      121 ttatggcgcg gaagctttat cggatcagga gcttttggca atcttactgc gtaccggtca
      181 acacccttat agtgtgatgt cgattgctgg aaatctgtta aaaacgtttg gaggactggc
      241 ctctttacga caggctactt tgcatgagtt agaagagatt cgagggatag gacgcgtgaa
      301 agcaatcgaa atcaaagcct tgatcgaatt aggtcgacga attcagacgg atcatgaaag
      361 agcggctcca ccggttcgta ccagctacga attggcccag caattgattc tggaaatgaa
      421 agatcacaaa caagaacatt tggtttgtat ttatttggac acgaagaatc aagtgatctt
      481 aaaaaaaaca gtgttcatcg gttcgctgaa tcagtcagtc gctcatccta gagaaatttt
      541 ccattatgct gtacgttatt gtgcagcaag gatcgtttta gcgcataatc atccttctgg
      601 taatgttatt ccatcgcagc aggatatgaa ttttaccaaa aggatacaaa agtgtggaga
      661 aatgatgggg atcactgtac tggaccactt gatcatcgga cgcaaacgat attttagttt
      721 gagagaagaa ggaatgatgg aagagaagta agttgctttc ttgcaaaaaa aatgaagcaa
      781 gtgtacaatt atattgtaat tttgtttggg aatagaagtt gaaacatctg ttgcacctct
      841 atcgtacacg agcaatgtta caatttaaat tagtgctttt ggcacgagga ggatattatt
      901 atggaaaacg gaacagtaaa atggttcaac gctgaaaaag gatttggatt tatctcacgt
      961 gaagatggaa gcgacgtatt cgttcacttc tcagctatcc aaggtgacgg attcaaaaca
     1021 ttagaagaag gtcaagcagt gactttcgac gttgaagaat cagaccgcgg cccacaagct
     1081 gcaaacgttg taaaagctta attttatctg taaatcaaat tgatggtaga catcaatttt
     1141 tacgagagaa caaagcgagt aacaaaaata cctgccacaa gagacaattt ctcttgtggc
     1201 aggtattttt ttgtatgaaa aagtaaataa atttaagagg ctatgaaaga gccaccttgt
     1261 atcaaggaat aagaacaaac aaaaatggct tctcatgttt ttcatttgtt ttctcttata
     1321 ttcaggacaa ggtgctgatt tcataacctc ttatttattc ttcatctggt gtcattacca
     1381 ttggcaaaat cattggatgt ctttctgtac gttcgaataa gaaaggctga agcgcgccag
     1441 tcatcgcttc tttcaattta tattctgaac agtcaggtcc attcaaacat tctcttaaag
     1501 cgtggaataa gattcgctga gcttcattga tcatttctcc tgattctcgc atgtagacaa
     1561 atccacgaga gagaatatct ggaccagcca agatttcttt tttcttgata tccactgtcg
     1621 cgacagctaa tacaagacct tcttctgaaa gcaggtgacg gtcacgtaaa acgacattac
     1681 caatatcacc gatcccgcta ccatcgatat acacatctcc agccacaaaa tgcccagcag
     1741 gacgcgcaga atcgctagtt aacgctaaaa catcgccatt ttccatgatg aagcagtttt
     1801 ctgcaggaac acctacatct tgtgctagag aagcatgtac tttcaacatg cggaattctc
     1861 cgtggacagg aataaaatat tttggcttca tcaaacgaag cattaatttt tgttcttctt
     1921 gtccaccgtg accggaagta tggatgttat tgatttttcc gtgaatcact tctgcaccag
     1981 cttcagacag caaattgatc aaatgattga cacttgttgt attaccagga ataggtgaac
     2041 ttgaaaagat cactgtatcg tctggctgga tttggatttg acggtgagta ccattcgcga
     2101 tgcggctcaa cgcggccatc gcttctcctt gagaacctgt acataggatc atcgtttcat
     2161 tggcaggcaa gcgattgatt tcgtgtgcat ctacaaatgt gccgtctgga actttgatat
     2221 atcctaaacg ttgaccgttg acgatggcat tttccatgct tcgtccgaaa acagcgattt
     2281 tacgaccagt tgccacagcg gcatctgctg cttgctgcaa acggaagatg ttagaagcaa
     2341 agctggcaaa aatgatacgt ccttcgattt ttgaaaagat cttcaaaata gaatgcccaa
     2401 tcgttttttc tgatttagta aatgttggaa tctcagcatt tgtactatca gataaaaggc
     2461 acaagactcc ttcttccccg atacgagcca ttttatgcaa gtttgctggt tcgccaacag
     2521 gagtgaaatc gaatttgaag tctccagtcg ctacgatatt tccagaaggt gttttcacca
     2581 cgatccctaa tgcatctggg atactatggg tcgttctgaa aaagctgaca gaagtctttc
     2641 taaaacggat gatcgtatct tcattgatct catggagctc tgcatctcga agcaagccat
     2701 gctcatctaa tttgtttgtg atcaacgcta atgcgagagg accagcatag ataggaatat
     2761 ttgcttgttt cagcaagtag ggtattccgc cgatgtggtc ttcgtgcccg tgagtaatca
     2821 ccaaggcttt tactttgtgt aagttttgta tgatgtagct gtagtcggga atgacatagt
     2881 caattccaag taaatcgtct tccgggaatt tgatcccggc atcaatcaaa atgatttcat
     2941 cttggaattg taccccatac gtatttttcc cgatttcacc taatccacca atagcaaaga
     3001 cgcctgtctc gttatttttg atggaaagtt tcatattaaa actccactaa tgagaaatca
     3061 gcattttctt tttcgtactc tagatgactt ccttctagaa gttgaacgaa ttcaatattg
     3121 tatggggtat tttcttccac acgttggcga gcgtctacat cgctttttgc ctcaatgtaa
     3181 agagattttg tttcttcccg tttagggttg cgtacttttg tttcttgata ataaacttta
     3241 tagatcattg ttttgtctcc tttatctgcg taaagcagtg atttagctag ttgatttttt
     3301 tagacaacac aaaacgaggg gacaagccac ctggtttttc atatgctttg actagaatac
     3361 tggattcgag tcaaagagac aagcctcgtt tttgcttatc tgttcggata ttttttcaac
     3421 cgtaccttct gtataaaaaa tatacaacaa ttaattgtta ttctagcata aaacagcaaa
     3481 taagtataga aatcaactag attgaaagaa aaaatcaatg tttatcatcc cgctataatc
     3541 ataatgactt ttttatgaga gaagtgttct attaataggc tcgttgtgct atcattttta
     3601 gtaatgaaaa tgtaacaaaa gattaaaagg agatgagaaa gtgaaactaa tgtggcatta
     3661 taccatgcgt tataagaaat tcttgttttt caatttcata tgcgtatttg gttttatttt
     3721 gattgaatta ggacttccta cgattttagc acgtatgatc gatgtaggaa tccgcaatga
     3781 tgattttgat tatgtgaagc aacaagggat atttatgatc gttattactg taatcggtat
     3841 tattttgaat atcttgttag gttattttgg ttcgaggatt acgactaata tcgtagcaga
     3901 tattcgagat gatttgttcg aaaagatcca aagttattcg catcaggaat acgaaaatct
     3961 aggcgtatct tcattgatca ctcgtacaac aaacgatgct tatcagatca tgttgttcct
     4021 gcaaaatatt ttacgtatcg gttttatggc accaatgatg ttcattgtca gtttgtacat
     4081 ggtcatgcgt acaagtgtga cactcagttt gtatgtagta ggtgcattac ctttactatt
     4141 attggcagtc gtagcgattg ctaaggtgtc tgaaccgtta tctaaaaagc aacaaaaaaa
     4201 cttagataaa atcaacagta tcttgcggga aaatctttca ggattgcgtg tgattcgtgc
     4261 attcgtcaat gaaaagtttg aagaaaaacg ttttgctgta gtcaatgaga attatgcttc
     4321 tagttcaaaa agcttattcc gtttgatggc tgttgcacaa ccgggttttt atttcttatt
     4381 taatatcgta atggtattga tcatctggag cggggcagtg caaatcgatg caggcgagct
     4441 tcgtgtagga gatttgattg cgtttatcga gtatatattc catgccttgt tctcattcat
     4501 gttatttgct agtgtgttca tgatgtatcc aagagcagct gtttcagctg gacgtatcca
     4561 agaagcccta gatgccaaac cttcgattgt agaagatcca aatggtatat ctgatacaaa
     4621 aactaaaggg tatatcgaat ttcaaaatgt aacatttgct tatccaggcc attcgcaaga
     4681 gccagtgata cgaaatgtca gttttacagc ctctccagga gagaccgttg cttttattgg
     4741 aagtaccggt agcggaaaat ctacgttgat ccaattgatc cctcgctttt atgatgtgag
     4801 tgaaggaaag atcttgatcg atggagtaga cgtgagagat tatcggctaa gcaagttgcg
     4861 agataagatt gggtttattc cgcaaaaagc gctgctattt actggaacga ttgcagataa
     4921 tttgcgatac gggaaagaag atgctacgca agaagaaatg gaacgtgcag ctgagattgc
     4981 tcaagcttca gaattcattt ctcgaaaacc agatggattt aatgaacatt tatcagaagg
     5041 tggttccaat ttttcaggtg gacaaaaaca acgattggca atagccagag ccatcatacg
     5101 ccgtccggaa atctatattt ttgatgacag cttttctgca ttagattatc aaacggatgc
     5161 gaagttacgt gctcgtctga aaaaagaaac gacagaatct actgtattga tcgtagcaca
     5221 acgtgtggga acgatcatgc atgcagataa gatcatcgta ttaaatgaag gagaagtcgt
     5281 cggtatgggg acgcatcggg agttgttgga aaaatgcccg atttattatg atattgccgc
     5341 ttctcaatta tcaaaggagg aattagcatg aaaaaaacct tttcttcctt aaaacgttta
     5401 gctcgatata tcagaccgta tcgtgagact tttcttttag tgatcctatt tacagttctg
     5461 acagtggctt tcaacgtggc tttgccatat gttacaggac ttcctactac agaaatcagc
     5521 cgtaatattg ccaatggcga atcaatcaat ttttcttata tattcaaatg tctcatttgg
     5581 atcacagttg ttggaatagg gtattgtatt tcgcaattac tttctggtgt tttgatgaca
     5641 aatgtcgttc aaagtgccat gcatgatttg agaagagaca tagaagaaaa aatcaatcgt
     5701 cttccggttt catatttcga taaaaaccag caaggaaata ttttgtctcg tgtgacgaat
     5761 gatgtagatg cagtcagcgg tgctttacaa caagctttta ttggtatcgt aaatgcgata
     5821 ttgggaatta cgttggctgc agcaatgatg ttttatatcc agccgatgat ggcgctgatc
     5881 tctatgatca tgattccaac atcgatctgg atctcaaaac gagtgatcaa tgcttcgcag
     5941 aaatattttc aaagcatgca gaattctctt ggtgagctaa acggctacgt ccaagaaaat
     6001 atgacaggtt ttagtgtatt gaaagtatat ggacgcgaaa aagaaacatt ggaaggattc
     6061 aaaaaagtta atcacagctt gaaatacttt gggtttcgtg cagcattcat ttctggcttg
     6121 atgatgcctc tagttcaatt aactgcctat gctacttata ttggtatggc tgttctagga
     6181 agcttttatg ttattactgg ggcgatcgtt gtcggtcaac tacaagcatt catccaatat
     6241 atttggcaaa tcagtcagcc aatggggaac gtgacacaat tgtcttcagt tttgcaaagt
     6301 gcatcagcgg caacgaaacg tgtattcgag attttagatg agccagaaga aaaagaaaac
     6361 aaagtagatg cttcactgcc agaaacgatt ttaggagatg tgacattcga tcatgtcgat
     6421 tttgcatatg atccgaagaa accattaatc caagatttga attttgaagt gaaagctggt
     6481 caaaccgtag ctgtggtcgg tccgaccggt gccggcaaga cgacattgat caatctattg
     6541 atgcgttttt atgacgtaga caaaggagca ataaggattg atggaatcga caccaaatca
     6601 atgagtcgca gtgatgtgcg ttcgctattt ggtatggtct tgcaagatgc atggctttat
     6661 caaggaacga ttgcagataa tatccgcttc ggaaagcttg atgcgacaga ttatgaagta
     6721 gttgatgctg cgaaaacagc aaacgtagat cattttatcc gaacgatgcc agatggatat
     6781 gatatggcca tcaattctga gggagacaat gtgtctttag gacaaaaaca gttgctgacg
     6841 attgcccgag cagtggtctc cgatcctaaa atcttgattt tagatgaagc aacaagttca
     6901 gtagacaccc gtttagaagc gttgattcaa aaagcaatgg atcgcgtgat ggagggacgg
     6961 acaagtttcg tcatcgctca ccgtttatcg acaatccgtg atgcagatct gattctagtg
     7021 atggatcaag gtcagatcat tgaaaaagga acgcatgaga gtttattggc acaaggtgga
     7081 ttttatgaaa aactgtataa cagccaattt gctgaggaag cagaataagt catataagaa
     7141 taaaacagtt taaaacttat tttttgttct ctattcttga taatggtaaa gtaaagttat
     7201 caagaaaagt gaggacacgg agatggaaca gtttttaaat gaaaaaatta ctgaagccaa
     7261 aagagaagtt gatcgtttaa cgcacgtctg tcagacgaag ccttttgatt cagctgattt
     7321 ttttgaaaat gagtttcaaa tcgaacgctt ggcagcacaa atagaagtgt acgaattaat
     7381 tttaaaaaaa gtaatagaag aaaatataga tgtacaataa aaacaggtaa aacgtcagtt
     7441 accagatact gtaaatagac atctggcctg agcaggtttt acctgttttt tttcttatga
     7501 aataaatata tgctatgata cctttattca ataggattag tttctacaaa aaatgaaagg
     7561 agaaaaataa atatggaacg gtttatagca tcaaatgcca cagtgattgg tgatgtgaca
     7621 ttgtcagaag atgtgacgat ctggtatcaa gctgttttac gcggggacag taattggata
     7681 aaaataggac aaagaacgaa tatccaagat ggtacaatca tccatgtcga tcatgatgca
     7741 cctgtagata ttgcggagaa tgtgacggta ggacatcagt gcatgctaca tggctgtaca
     7801 atcgaaaaag gggcgctgat cgggatgggg accacgatat tgaatcatgc ggttattggt
     7861 gagaatagtt tgattggtgc tggatcatta gtaacagaag gaaaagttat tcctcctaat
     7921 gtcttggctt ttggtcgccc agctaaagtg atccgtcctt tgacaaaaga agaaatacaa
     7981 aaaaacaaag aaaacatcca gcattatgtc gagataggtc ggatgcatgc gcaaggaaaa
     8041 ttcaaggagc aaagataggc agtagcatcc ataaaataaa aaaggatggt cagtttgagt
     8101 tgaccatcct tttttatttg gtttagataa gagagcttaa aaaagcttcc tttgaaacag
     8161 attaatttaa tgaagaactg ttttccatga cttcatcgat caatccatat gcttttgctt
     8221 gttcagctgt catatagttg tcacgatctg tatctttttc aatcacttca agcggttgtc
     8281 cagtgcgttc agctaaaatt ttgtttaagc gttcgcgtgt ttgcaaaata tggcgtgccg
     8341 caatttcgat ttcagttgct tgcccttgag cgccgccaag aggttgatgg atcatgatct
     8401 cagcatttgg taaagcaaat cgtttacctt ttgtaccagc tgtcagcaag aagcttccca
     8461 ttgaagcagc catacccata acaattgttt ggacatctgc tttgacaaag ttcattgtgt
     8521 cgtaaatagc catacctgct gtaactgaac caccaggaga attgatataa agatagatat
     8581 ctttttcaga atcttgagcg tctaagaaaa gcaattgggc gatgatcgaa ttagcaagat
     8641 catctgttac ttggccgctt aacatgatga tacggtcttt caataaacgt gagtagatat
     8701 cgtaagctct ttcacctcgt gaagattgtt caatgactgt aggaattaaa ttcataatag
     8761 agttcctcct tgatttcgtt tagaaatagt gtaccataat ttagtttcta atacctaaaa
     8821 tgtagtatac atttttggtc aaaaaaggtc aaataaaaaa cttaggcaaa ttagtcgcat
     8881 aaacccgctt tatggtatga taagcagaga aacaaaacaa ggaggaacaa cagattgata
     8941 gttggtatta ctggccttat cggagtgctg acaggtgctg tagttgtcta tatcggcatg
     9001 atactgcaaa tgcgtcactt ggaaaataaa gaacagcgta gacgtgaatt ggaattgaaa
     9061 gtcaaagaaa tcgagacgct gaatcaatta aataaaaaga tcaacgagat cttgcaaaaa
     9121 cggacaacgc tgatggaaga atatgtcagc tttgatgcat ttgatgattg ttatatcacg
     9181 attgacgatt ttgcgtatct ccaatcattt tcagcgcaaa atagttttta tctgccaaat
     9241 tatattttag acgagctatt taagaatatc tcccatcgaa aagtgatttt atctccagat
     9301 gaaacaatga agattggtgg atatgcttat aaaggcggac gcgtgatttt agagaatttt
     9361 acggatagtc tgactgaaat gatcaatgaa aaaaaagctc agttgaagac tatgacgcgc
     9421 cagccgattg gtttcttctc gaaagatgat gtttggagct gatattttga agcattgccg
     9481 ttgcacatga gagcgtggta atgctttctt tttttcttaa tgatacaata gaactgaacg
     9541 gagtgaagaa agatgttaca aatctatgcc catcgcggaa gcagcggcac ccacccagaa
     9601 aatacgcttc ctgcatttgc agaagctgta cgagtaggtg cagatggtat cgaactagat
     9661 gtccatctaa gcaaagatgg ttatttgatc gttatgcacg acgaagaagt ggaccggacg
     9721 actaacggta aagggctgat tcgagaaaaa acattggaag aactcaaaaa attgaatgca
     9781 gggggcaaat tcagcaaaga atttccagca gctaaagttc ctgaattgag agaagtgtta
     9841 ggactgctcc tccaaaaaaa ttaccgaggg atgctaatga ttgaaattaa aactgatcag
     9901 tatgaatatc ccggtatcga agaaaaatta gccgaaacat taaaaagtag agattggcct
     9961 tttcaacatg catattgcag ctttcatttg gattctttgg aaaaaatcgc attactggaa
    10021 ccggagatac agctggactt attgatgagt acttctgata aaaagatcca actagcagca
    10081 gaaacccctt atatagaagg agtacatcca aaaaatactt ggttgttcga acatccagtc
    10141 caggcagctg attttccaaa agcaattcgt gcatggacga ttaacgatga agaagaaatg
    10201 gccaaatgtc aagaactcaa aatagatgga ataataactg attttccgga gaaagctctt
    10261 cggtggaaac aatcaacgaa aagtaggtaa aatatggaaa aagtattagt gatcgtcggt
    10321 ccgacagcag tcggtaaaac agcgctaagt gtggaactcg cacaactatt tggaggagag
    10381 atcatcagtg gtgattcatt gcaaatctat aaaaatctgg atatcggtac agcaaaaatc
    10441 aaagaagaag agatgaataa tgttccgcat catttgattg atgtgatcga acctactcaa
    10501 cgttactcag ccgctgattt tcagcattca ggtcgacaat tgattaccga gattgcgaac
    10561 cgagggcacc tacctatcat agctggtggt acgggtctct atatccaatc ccttctatat
    10621 gattatcagt taggagcgac agaagaagcg aatcctgaga ttagaaaaaa gtatgaaacg
    10681 ttagctgaaa caattggttc ccaagcatta tggaatcagt tgaaagaaaa agacccggca
    10741 gctgctgaga aaatccactg gaacaatcaa cgcaaagtga taagagctct ggaagtttta
    10801 gagacgacag gacatagtat cacggcacct aaagaagagc caaagcaatt gtatgattac
    10861 ctcatgattg gattagatac gaagcgtagt attctttatg aacgaatcaa tcaaagagtc
    10921 gatttgatgc tagcagaagg tttagtagca gaagcgcgta aggtgtatga gcttggagaa
    10981 gtccaggcca gtcaaggtat tggatataaa gaatttttcc cttattttga agggaaagaa
    11041 acattggaag aagcaacaga acaagtcaaa ctacattcga gaagatacgc gaaacgtcag
    11101 ttgacgtggt ttcgtaaccg tttggatgca cagtggtttg atcttataag tgagccaaat
    11161 caaaaaagtc agattgagca aacaatcaaa aaatggctgg aggcggaata aaatggaaga
    11221 aaaagtgatc atcgtagggg tagaaaccga agcaaaccag cgttactttt cagagtcaat
    11281 ggaagagcta gttcagctga cgaataccgc atcaggagaa gtcgtattca caattacaca
    11341 aaagagacca caagtggatc ggcaaacgat tatcggtaaa gggaaattac aagaattagt
    11401 tcaacaagcg gatgcgcatg aagcggattt gatcatcttt aatcatgagc taactccgag
    11461 acagagccaa ctgattaccg atgctgttgg tcttccggta attgatcgcg tgcagcttat
    11521 cttagatatt tttgcgatgc gtgctcgttc gaaagaaggg aagctgcagg ttgaacttgc
    11581 tcagttagaa tacctgctgc ctcgtttagt agggcaaggg aaaacattat ctcgtcttgg
    11641 cggtgggatc ggaacaagag ggccaggtga aacaaagtta gaaacagacc gccgacatat
    11701 acggaataaa atttttgctg ttaagaaaga attgaaagaa gtagaagctc atcgagagcg
    11761 caatcgccaa aaaagaaaaa atagcgagat ttttcagatt ggacttattg gctacacgaa
    11821 tgccgggaag tcaactatct tgaatctatt gacacaagcg gatacttatt cgaaagatca
    11881 attgtttgca acgttagatc cattgactaa gagatggcgt tttgctgaag gatttgagat
    11941 tactgtaacg gataccgtcg ggttcatcca agacctgcct acgcaactga tcgatgcttt
    12001 ccattctaca ttagaagaaa gtcaaaatat ggatcttttg ctacatgtag tcgatgctag
    12061 ttctccagat cgtattttac aggagcagac tgtcttaaaa ttgatggatg aactgaatat
    12121 gaaagaaatg ccgatactga ctgtttacaa taaggcagac cagatcgacc cagcgatgtt
    12181 cacgcctacg ctattcccaa atgtattgat ttctactcag acgaaagaag gtaagatggc
    12241 gttgatcgaa gcaatcaaac gtcagctgat ggaactaatg accccttata cactttctgt
    12301 gccaagcaac gaaggacaga aattaagcga gttgagaaga caaacaatgg ttttggacga
    12361 agcgtacatg gaagaaacaa acgaatatga agttaaaggg tttgcaaaaa ccaattcaaa
    12421 atggttaaga aaaaaccaat aaaattttat ttttttaa
//
LOCUS       DACCCR010000076        12318 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCCR010000076 DACCCR010000000
VERSION     DACCCR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.083"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..572
                     /locus_tag="IVK93_002187"
     CDS             <1..572
                     /locus_tag="IVK93_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6387464.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IVK93_002188"
     CDS             576..1217
                     /locus_tag="IVK93_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387465.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IVK93_002189"
     CDS             1214..2131
                     /locus_tag="IVK93_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6387466.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IVK93_002190"
     CDS             2128..2799
                     /locus_tag="IVK93_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387467.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IVK93_002191"
     CDS             complement(3340..3828)
                     /locus_tag="IVK93_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP6387468.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IVK93_002192"
     CDS             complement(4029..4295)
                     /locus_tag="IVK93_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6387469.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IVK93_002193"
     CDS             complement(4295..4558)
                     /locus_tag="IVK93_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6387470.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4551..4823)
                     /locus_tag="IVK93_002194"
     CDS             complement(4551..4823)
                     /locus_tag="IVK93_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387471.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5034..5219
                     /locus_tag="IVK93_002195"
     CDS             5034..5219
                     /locus_tag="IVK93_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6387472.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IVK93_002196"
     CDS             complement(5361..7184)
                     /locus_tag="IVK93_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP6387473.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IVK93_002197"
     CDS             complement(7782..10487)
                     /locus_tag="IVK93_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP6387474.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IVK93_002198"
     CDS             complement(10793..11740)
                     /locus_tag="IVK93_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP6387475.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IVK93_002199"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IVK93_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP6387476.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
BASE COUNT         3529 a         2563 c         2330 g         3896 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttt
//
LOCUS       DACCCR010000077        12093 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCCR010000077 DACCCR010000000
VERSION     DACCCR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.084"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            597..1925
                     /locus_tag="IVK93_002200"
     CDS             597..1925
                     /locus_tag="IVK93_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP6387477.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2127..2960
                     /locus_tag="IVK93_002201"
     CDS             2127..2960
                     /locus_tag="IVK93_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP6387478.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2976..3713
                     /locus_tag="IVK93_002202"
     CDS             2976..3713
                     /locus_tag="IVK93_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP6387479.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3868..4836)
                     /locus_tag="IVK93_002203"
     CDS             complement(3868..4836)
                     /locus_tag="IVK93_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP6387480.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5068..5901
                     /locus_tag="IVK93_002204"
     CDS             5068..5901
                     /locus_tag="IVK93_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP6387481.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5935..6774
                     /locus_tag="IVK93_002205"
     CDS             5935..6774
                     /locus_tag="IVK93_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6387482.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6800..7360
                     /locus_tag="IVK93_002206"
     CDS             6800..7360
                     /locus_tag="IVK93_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6387483.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7559..9280
                     /locus_tag="IVK93_002207"
     CDS             7559..9280
                     /locus_tag="IVK93_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP6387484.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9445..10587
                     /locus_tag="IVK93_002208"
     CDS             9445..10587
                     /locus_tag="IVK93_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6387485.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10603..11814
                     /gene="thiI"
                     /locus_tag="IVK93_002209"
     CDS             10603..11814
                     /gene="thiI"
                     /locus_tag="IVK93_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP6387486.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4129 a         2030 c         2510 g         3424 t
ORIGIN      
        1 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
       61 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
      121 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
      181 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
      241 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
      301 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
      361 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
      421 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
      481 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
      541 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
      601 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
      661 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
      721 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
      781 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
      841 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
      901 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
      961 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     1021 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     1081 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     1141 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     1201 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     1261 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     1321 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     1381 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     1441 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     1501 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     1561 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     1621 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     1681 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     1741 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     1801 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     1861 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     1921 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     1981 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     2041 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     2101 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     2161 taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa
     2221 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     2281 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     2341 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     2401 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     2461 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     2521 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     2581 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     2641 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     2701 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     2761 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     2821 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     2881 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     2941 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     3001 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     3061 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     3121 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     3181 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     3241 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     3301 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     3361 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     3421 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     3481 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     3541 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     3601 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     3661 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     3721 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     3781 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     3841 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     3901 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     3961 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     4021 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     4081 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     4141 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     4201 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     4261 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     4321 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     4381 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     4441 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     4501 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     4561 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     4621 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     4681 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     4741 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     4801 cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat
     4861 tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga
     4921 ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa
     4981 ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa
     5041 ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa
     5101 catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc
     5161 ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg
     5221 ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt
     5281 caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat
     5341 gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca
     5401 tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag
     5461 ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa
     5521 cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag
     5581 ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt
     5641 cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa
     5701 agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta
     5761 gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg
     5821 gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca
     5881 acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc
     5941 aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     6001 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     6061 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     6121 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     6181 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     6241 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     6301 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     6361 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     6421 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     6481 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     6541 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     6601 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     6661 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     6721 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     6781 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     6841 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     6901 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     6961 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     7021 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     7081 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     7141 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     7201 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     7261 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     7321 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     7381 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     7441 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     7501 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     7561 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     7621 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     7681 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     7741 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     7801 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     7861 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     7921 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     7981 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     8041 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     8101 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     8161 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
     8221 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
     8281 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
     8341 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
     8401 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
     8461 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
     8521 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
     8581 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
     8641 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
     8701 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
     8761 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
     8821 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
     8881 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
     8941 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
     9001 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
     9061 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
     9121 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
     9181 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
     9241 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
     9301 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
     9361 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
     9421 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
     9481 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
     9541 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
     9601 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
     9661 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
     9721 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
     9781 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
     9841 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
     9901 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
     9961 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    10021 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    10081 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    10141 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    10201 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    10261 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    10321 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    10381 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    10441 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    10501 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    10561 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    10621 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    10681 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    10741 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    10801 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    10861 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    10921 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    10981 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    11041 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    11101 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    11161 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    11221 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    11281 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    11341 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    11401 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    11461 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    11521 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    11581 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    11641 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    11701 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    11761 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    11821 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    11881 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    11941 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    12001 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    12061 agtgggcagc ttcaagaaat aagaaggaat tta
//
LOCUS       DACCCR010000078        11958 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCCR010000078 DACCCR010000000
VERSION     DACCCR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.085"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(62..424)
                     /locus_tag="IVK93_002210"
     CDS             complement(62..424)
                     /locus_tag="IVK93_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6387487.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(435..1556)
                     /locus_tag="IVK93_002211"
     CDS             complement(435..1556)
                     /locus_tag="IVK93_002211"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP6387488.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(1572..1922)
                     /locus_tag="IVK93_002212"
     CDS             complement(1572..1922)
                     /locus_tag="IVK93_002212"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP6387489.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(2052..3563)
                     /locus_tag="IVK93_002213"
     CDS             complement(2052..3563)
                     /locus_tag="IVK93_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6387490.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(3885..5237)
                     /locus_tag="IVK93_002214"
     CDS             complement(3885..5237)
                     /locus_tag="IVK93_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP6387491.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(5390..6466)
                     /locus_tag="IVK93_002215"
     CDS             complement(5390..6466)
                     /locus_tag="IVK93_002215"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP6387492.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            6662..8407
                     /locus_tag="IVK93_002216"
     CDS             6662..8407
                     /locus_tag="IVK93_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP6387493.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(8687..9124)
                     /locus_tag="IVK93_002217"
     CDS             complement(8687..9124)
                     /locus_tag="IVK93_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP6387494.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(9108..10481)
                     /locus_tag="IVK93_002218"
     CDS             complement(9108..10481)
                     /locus_tag="IVK93_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387495.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(10803..11291)
                     /locus_tag="IVK93_002219"
     CDS             complement(10803..11291)
                     /locus_tag="IVK93_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6387496.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(11313..11915)
                     /locus_tag="IVK93_002220"
     CDS             complement(11313..11915)
                     /locus_tag="IVK93_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6387497.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3430 a         2600 c         2157 g         3771 t
ORIGIN      
        1 tttttttacg cacagctggt ccttttttat atgtaaaggc tgcatggtta cttcgctata
       61 tttatttagt aacaggagtc agtgcttcga tacccacact aattttcaat gctcggtcaa
      121 cttctgccat aatgggtcga tccaaatggc agaccttttc tttcaatctt gatttatcga
      181 tcgttctgat ctgctccaac aggataacgg agtctctttc tattcctgtt ccagtagctt
      241 tgatcccaat gtgagtcgga agcttaggtt tagccatttt tgccgttatg gccgcaacaa
      301 tgatcgtcgg gctaaaatgg ttacctagat tattttgaat aaccaaaact gggcgtgttc
      361 cgccttgctc cgatcccacg actggagaaa gatcagcaaa ataaatatct cctcttttta
      421 ccataggatc ctccttattc ttgatattct cttggtatcc gctgtcctaa tccgcaagcc
      481 acttcatagt ggatcgtacc taattgatct gctatatcct gcatagtgat ttctttgtct
      541 gcatttttgc cgataagcgt cacttttgtt ccgacaggaa attcttgagg caatcggatc
      601 atcagttgat ccatacatac acgcccaatc gtttcacagt agttcccttc taccaataga
      661 gaaaaaccct gcatctttcg tggccagcca tctgcataac cgatcggtat cgttccgatc
      721 cactctgctt caggagtgat atatgtttct ccatagccga ttccttcacc tgctgggagt
      781 tttttgactt gaatcaattc agaaaccagc tcaagtgctg gctgcaatgg ataaacttcc
      841 ggtagtgcgt gcccagaggg attaagtccg tacattgcta ctccataacg aatcatattg
      901 ccgattgttt catcatgcca taaagccgtt gcactattgc tgacatgtac atatcgagga
      961 agttcgggaa gttttttcag cacttctata aagcgttctt tttgcagatt ccaatatgta
     1021 tcatctgcct gatctgctgt agaaaaatgg gtaaataaac cttcccattc taatacacga
     1081 ctttctttga tgaatgcagc tgcctctttt acttcttcag gtgtacaaaa tccgatacga
     1141 cccatcccag tgtctgcttt tagatgaatc gaaagaggcg tctctagctt acttttctct
     1201 aaaacagcag ctgctgcttg caaccattcc cgtgtacctg cagttactga taaatcatga
     1261 gcaacagcta cagtcagtgc gtcgtaagga accatattca agataaggat gggctgagta
     1321 aatccagctt ctctcaactc gactccttca tctaaatttg aaacacaaaa gcccgaagct
     1381 ccacctgcaa ctgctgcctc agctgtttca atagctccat gaccgtatcc gttcgctttt
     1441 actactgcaa acaattcttt tccttgcgga agtcttgcaa cttcatttgc tacattttca
     1501 gttatagctt ttttatgtat cactgctttc gttgggcggt gccaagcgac aaccatcgtt
     1561 tttccttctt tctagtcttc caaaataatt tgtgcaaaag ctgtttcatt tgtatgagta
     1621 atactgaccc atacattgcc ttcatgaggt gaacgcgtca caacaggctg accattttca
     1681 ttttttaaaa tctcgacatc ttgaaaggta acttttccga tcccagttcc ccaagcttta
     1741 gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt ctacttggcg atgaaaaggc
     1801 aaggactgga atagtttcat ttcatcagag gtcaagattc gtgcaatgaa ttttggtttt
     1861 tcttcgatca gccgagtgat cctaggaagt tcgactgcat cgatcccaat tccttttatc
     1921 atatgctttt ctcttttcaa taaatttaga acactctcat tctaccaaat tataaccatt
     1981 ttgccacaaa taagtccaca aataacaaaa atgaaagttt ctcttaaaaa agagaaactt
     2041 tcattttatg tttaatcgtt atttgaacgg attacaaatc cgcgtttttt atcattgtga
     2101 cgtttaccgc catctttttg gctgttatta tttttgctat aaccgccttt tgatttgttt
     2161 ccacgataat taccgccgtc acgacgatta cggttgtttc cgccaccacg tttgccattt
     2221 ttattgtatc cttttttctg cattggcaat gggcgttctg gtgtgatctt cactggcact
     2281 gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg caactaaatc ttgtgcagaa
     2341 tattcttcta ataatttatc tgctgtttgc aggtatttat ctagaccatt ttctgcaagt
     2401 tttgtttcga tttgttcaac tgcagcacct aattgacctt tgaatgcttc tttttcggtt
     2461 ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt caatcacatg caagtagtcc
     2521 atttcatttg gtgtaacgaa agtcacagac ataccgcctt ttccggcacg gcctgtacgt
     2581 ccgatacggt gaacgtagct ttctggatct tgagggatat catagttgta cacatgagtg
     2641 acacctgaaa tatctaatcc acgtgcagca acgtctgtcg ctacaaggat atctaaatga
     2701 ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt gagacaaatc gccatggatt
     2761 ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca attcgtctac acggcgtttt
     2821 gtacgtccga agacgattgt taattcaggt gtttgaacat caaacaagcg agtcatgata
     2881 tcgaattttt cgtattcttt tgcgcggaca tagtattgat cgatcaagtc agctgtcatt
     2941 tctttcgctt tgatcttcac atgatgtgga tttttcatga atttaacgcc aatattcttg
     3001 atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc tttgatcagg aacttgtgaa
     3061 atgatttttt cgatatcttc caagaagccc atattcaaca tttcatctgc ttcatccaaa
     3121 actaatgttt gaacggttcc aagtttcaat gtgtggcggt taatgtggtc aagcatacgt
     3181 cctggtgtac ccacaacgat atgtggacga tctttcaagc cacggatctg acggccaata
     3241 tctgctccgc cgtaaacggc ttgtacacgt acttttttgt cgcgccctaa acgatataat
     3301 tcttcttgtg tttggatagc taattcacgt gttggtgcga taactaaacc ttgtaattcg
     3361 tgacgatctg gatcgatttt ttctaacatt ggtaatccaa atgcagctgt tttccctgta
     3421 cctgtctgtg cttgtccaat aacgtctttt ccagctaatg ctagtggaat cgtttctgct
     3481 tggataggcg ttgcttcttc aaaaccagcg cgttcgactg atttcaacaa ttctggtgat
     3541 aattctagtt ctttaaattt caaatgaatc ctcctatatg gttgttaact gaggatgtag
     3601 cttttttgct accccgtcga tttgttcggt caaagtgtat tgtttctttt tgacagtggc
     3661 cacgcacgtt tttatccatt cttagcccct gtggcaccac cgaaaaatca ctttgcgaag
     3721 tttaggcatt agaaaagcca aacgggtcgc gttcagcttt atcattatta tgacatctgg
     3781 cttcaacaag ttgtttctat ggcaaaacca gtccaatttc tcttcatctt tcggcaatag
     3841 gttattctaa catagttttt cgttttctgc aagtttacag atttttattt catttgctga
     3901 agttgttcta ccacttcaaa taagcccatg ctgttgctgc cttttagcat aatactatct
     3961 gttgctgcaa gattttgttt gatagcatca atcaatttat tcttttcttt ttcaaaatag
     4021 aacacaggta gttgggggta tttttggttc aattcttctt ttagtgcatg catttgttcc
     4081 ccataaagga aaatcatggc gaatttctca ggatcgatat gttcactcat ttgcgcatgc
     4141 atatttaaag actctttccc taattccagc atatctgcta acacagcgat tctgcgtcct
     4201 tttgttggca gatttttaaa agtatctaat accagtccca tggctgttgg atttgcattg
     4261 tagacatcgc tcaaaatagc tgcaccattt cctgccgtta gccattcggt tctgttttga
     4321 gtgatagaaa catgggcgag cccttggcgt atctcaggta tggacagtcc aaagaagcgc
     4381 ccgatcgtat acgcaatcaa agcattcgtt acattataac ttccaggcag cgggatcgta
     4441 aatgtttctc cttctactac aaaatcggtt tgtgcttttc cttttgctgt cacttccgca
     4501 tgaacatcgc catttccaat accgaatgtt gtgactgttt gtactaatgt tgccgttaaa
     4561 ggtgtcagca acggttcatc tgcgggcaca acgagtaatc cgtctgatac aagtccgtca
     4621 gtgatttcca ttttggcttt agcgattcct tctcgcgacc caagattttc tatgtgtgct
     4681 tcaccgatca ttgtgattgc tgcagctttt ggttgagcca taagtgaaag ttccgtgatt
     4741 tcattggcgt gatccatgcc catttccaaa acgatctttt ctgtatcttg gggcatgtgc
     4801 aaaatcgtgt aaggaagacc aatattattg ttgtaatttc cttgcgtctt atatgttttg
     4861 tatttttcag acaagacaga cgcggttaga tctttcgtgg tggttttacc attgcttcct
     4921 gtaattgcaa cgacttctgc gcctactttt tccaagtaat aggttgccaa ttgctgcatg
     4981 gctttcaaag gatctgctac aggaataact ggaaaatttt ttggagcttg ttcaacaggc
     5041 aagctccaaa atgaagcaga tgctccgttt gtgatcgctt gctggataaa ttcatgtcca
     5101 tctcgcgtac cagcaagagg aacgaataaa cttccttcgg ttatcttccg gctgtcaaat
     5161 tcgacattgg taatctttgt cagtgcagag cattcgctgc ccacggcttg tgcaatttct
     5221 tgtattgtta gattcattga gatctctcct aaaaaaggtt gtgaaaaaag cgtttggcct
     5281 aaacggcggg ttcgcatgac tctcacttcc aagtaaattt ctctattcgt atttagaagc
     5341 tagaatgaac gcggttacgc aatcactcca gcctcttctt tatttctctt tattcatttt
     5401 tttcaaaaaa agattgacgc tgatggtatc gattcattcc taactggatc agttcttcaa
     5461 tcaaatcacc gtatttcaag cccatatttt cccataagag tgggtacata ctgaactccg
     5521 taaatcctgg catagagttt aattcattca ggaataattc atttttattt gtcaaaaaga
     5581 aatcgcaccg gctcaatccg cttccaccta acatcgtgta agctaacttc gcgtactctt
     5641 gcgctttttg ataaacttct tctggcactt cggctggaat ctgcatttcg attttattat
     5701 tgatatattt tgcttcataa tcatagaatg ctacgtcttt tacgacttca ccaggcaaag
     5761 tcgtccgaac gtcttcattt cctaatacag caacttcgat ttcgcgcgct tcaattcctt
     5821 gttcaacgat tgctcgagaa tcatactgat aggctgttgc taaagcattt tgcagctctt
     5881 ctcggttttc tgcttttgta atgccgacac tagaacccat attcgccggt ttgacaaaca
     5941 tcggataaag caaagaacct tcacattgat caaatacttt tttaggattt tctttccatt
     6001 gattcttaag tactggtaca taaggaactt gcggcacacc agcagcttgt aaaatatact
     6061 tggtcatgat tttatccatg gcacatgcac tggtcaatac gcctgcgccg acataaggca
     6121 tattcaatgt ctctaagaag ccttggatcg ttccatcttc cccgtttggc ccatgtaaaa
     6181 ctggaaaaac gatggctcct tcttctttga tttcgcccgg attgatcact tttcctgtaa
     6241 agccttcctc tgtctgtcca cttgggtccc atgaaagatg caagacatct ttgctggcag
     6301 gtttttctgt tagtaatgga cctttgaccc attgtccttc ttttgtaata aatacgagtt
     6361 gaacttggta ataattataa taaatggcat ttaaaactga aaatgcggaa agaatggaca
     6421 cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat cttcaaaaga ttgtcctcct
     6481 gtattctaaa tcgttcagtg atttactctc atattttagc acaaaacaaa aataaacgaa
     6541 taacttattt ataaaaaaac gagccagctt ctaaaagaac gagcgtcatt tattttttgt
     6601 cagctagatt atttgtcccg ttttttataa aaagttatga taaagaaaag gagggatgat
     6661 gatggcgcaa acaaatccac gcagccgctt ggcagaaatc attcgagtgt tcatcaatta
     6721 caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa caggtaaaaa aagcctttga
     6781 agaactaggc cccaccttca tcaagatagg acagatgctt tctgttcgag aagatttgct
     6841 ttcttctgcc ttcactcaaa cattcaaaac attacaagat agtgtaccta gcgacacatt
     6901 ttcaacggtc aaaaagacga tcgaaacaga actttctctt tcattatcag atatattcga
     6961 tgatttttca aaatcgcctt ttgcctctgc ttctatgggg caggctcatc gtgccaagct
     7021 gaagaacggt gacgcggtag tcgttaagat ccaacaccct aacatcgccg aagagatccg
     7081 acttgatctt caactatttg aacgagcgat ccctttgatc aaatacattc ctgaaactag
     7141 cgtagttgat ctaaaagggg tactccaaga agtaaaacgt tccttgatca atgaaatgga
     7201 cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa aagaacaatg gctggaaaga
     7261 gatccgcagc cccaagattt atgacgcttt ttgttcgaaa aaagtgatcg tgatggaaga
     7321 aatgtctggt aaaaacttga atcatttaat gaacgccgaa aacaaaactg aaacttttat
     7381 cactggaatt caaaacaagc agctgaaaca agaagttgca aagcttttag ttgagaactt
     7441 tatgaaacag gtgtttgatg atggtttttt ccatgctgat cctcatccag ggaatctgct
     7501 attccatgta ctaacaaaag aagaacagac acaagctagc cgaaaaactg aaacagtcca
     7561 tgaaaaagag ttcggttcct ttgcttttcg agcctccact tctgcagaag accctgttgc
     7621 gccatatacc atcaactaca tcgattttgg tatgatgggg catctttctg caggacttag
     7681 gcaaaaactc acacaagccg ttttagcgct gtatacgaaa gatgcttatc gaatcgaaaa
     7741 agcggtcctt cgactttgtc agcaagaagg ctcgtttgat gaaagtcggt tccaccaaga
     7801 actgacaagt tttcttgaac agtattatga ttctcctatt gatgaaatca acttgcaaga
     7861 agtattcaca catgtcgtga cgatttgtca tcaaaataat ctgcagttcg atcgtgatat
     7921 cacgcttttg ctgaaagcat ttgggacatt agaaggggtg atccgcgtcc tcgatcctga
     7981 agtttccctc atggaagtcg cttcgccttt tgctcaacat tattttctca ctcatctgga
     8041 tgtagaagat acattgaaac aaagcggatt agacttattg gaagggatga aggctgcgcc
     8101 taagatccct caacagctcc accatctatt agaaatgtgg acttcgggac aaggaaaagt
     8161 caatttggaa ttgaaaaagc aagacaaatt attgagcagg atcgaaagta tgatcaatcg
     8221 gcttgttttc ggtatgattt tagctgcatt gatcgtaggg tcctccctgc ttgtccaagc
     8281 agcacctgta gaaaacgcag aggtcgtttc cttattaggg atatttacgt atgctattgc
     8341 cgcattcgtc attatttttt tggcaatcga tgcattgatc caaatgtata agaagaggaa
     8401 aaagtagaaa aaggttgtga cagaaagaat cagcttcaag aaataagaag gggttttaca
     8461 taaattgttt ctcaaatttt tgtgaaaatt cggcttattt ttcgaagagg ctacttctga
     8521 aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc cagtaataag aacaatcaaa
     8581 aagggaacat ggaacacatt tatcctgttt ccctgttccc tttttgatgt attcgagcat
     8641 aaataaaata ccttcgttac ctactatctt ctgtacttgg taaaccttag tcaattgcct
     8701 tttttaacgc ttgacgaaaa gacatatgat ctttctccgt aaagggtttt ggaccctttg
     8761 tccttatgcc agcttttctt aactttccac tatgataacg ttgttctaga agcgtgccga
     8821 tattcgcttc gtcaaattct tttccagagt gatggaacac tcgcacaccc ttcggcaata
     8881 caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc attcttctta accataccga
     8941 cgattttata atccgcacta tcagctccgc ggtctacgta gacgatttgg acatgattag
     9001 cgtattcttt ggtcgtataa tggtccacac tggtcacgat aaccacgtca agaccatagg
     9061 attctcctaa ttttatgaca tcttctttta ctggagaacc atctccatca atgaatagcc
     9121 tcattctttt tcgccttctt ccataaatga taaacagtta ctaatccgct cagtaaaaga
     9181 ccaacattga tccattttgc caagataagc agttgtacac tttctatcgg cactgattca
     9241 tggttcagcg tgatcaataa tagatggatt ggctctccca tttcttttgc taaaacaaac
     9301 aaaagcgtca tcaataaaaa ctggtggatc acactccctt tattttgagt agtatacttg
     9361 acaacgatcc ggcgatccat tggaatagct gtatcagttt gataatattc cttatttagc
     9421 cagctgcctg ttactacgtg ccagaaacta agcagaaaga cacctatcgc aaaaccattt
     9481 ggaatcaaga ggaccaataa agaagaactc agtccaagca attgcatagc caatggagac
     9541 catgttaaac gtttcaaaat ccatggtccg actatttttg ctgcaatcaa gcctgctgca
     9601 taaggcaaat acataaaaat cgaaagtttt tctaatccgt acagtatgcc aacataaaaa
     9661 ctgctaaata aaaaaaggaa attccctagc attccattta gaaaagtaaa cagatttagc
     9721 cagcttggca acttccattc cttcttctgg tgtcctaaaa caaacacgaa caagatgaac
     9781 agcagcaaga aaccataaat agcaagatca aaaagccttt catcgaataa caatcgggca
     9841 tttctcaaaa gaaacaataa gccaaagaat atcaaaaata aaacaagctc tcgcgtagct
     9901 accagatttc ttttgatgtc tttgaaatcc agttcgtaac gcggataatg actaaccgta
     9961 tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc ttgctgcaat ctgagtcggt
    10021 gcaggcaact caatcgcttg atacaggaga aatagccaga tagctgcaaa taagtacttt
    10081 ttcccatcca tattgataaa accttgcgtc ttttcgtgaa aattgaccgt ggtattagca
    10141 ggtacaagcc atgctgcact cagccctagc aaaacacttg aaaaataaaa taaaggaaaa
    10201 taaaatacgc ctaatattcc aagcaatgaa ccagcaccac caatcagcag agcgatcatc
    10261 agcaacgtgt aactatctag accaaatcgt agactgttga ctaaaaatag tccagtcatc
    10321 cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc aattatgcga gtctgttagt
    10381 cccaaaaata aaagataagg aataaaaata accgtgttac ttaagataaa gggcgttgtt
    10441 tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca tgacaatggc tccttaaaca
    10501 gtcttataac taatctgctc ttcgatttta gcattaaagg ggaaataaac caattttaca
    10561 gaatatattc ttgtaaaaaa aaatacataa agaagcataa gcttctttct gcatttttac
    10621 ggtacttgat tactcaagca gacaagccct tctccacaaa agtcttgccc gacacccctg
    10681 catccggcag gtgataaaga tagaaatcaa ttatcctgtg cctccaaaat caattaggag
    10741 ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt tactttaaca cagttcctat
    10801 gtttatgcga gagctttttt cattttataa tcgaccattt gaaatccttg accgatatct
    10861 gaaataagtt cttctgtaat ctcaaatcct tcatgcttat aaacctcgat tgcttgatga
    10921 ttcccctgat tcactcgcaa aaagatctct ctttcctttg tttgcgcagc aagttcttca
    10981 taccagcgaa aaactgaaga agtcaatcct tgaccacgta ctttatttag caaatataat
    11041 ttacttaaat aaatttttcc atttatctct tcataagcag tgtaaccgac agtcttttct
    11101 tcgcttttta ataaaaagta ggacacacct tctgtcagtt ctttttgaat ctgttttttt
    11161 gactgataag ttttcagcat gtaatccact tgtttttgac caatgatcgg cacaaaagct
    11221 tccggccata cttcttgtat gactgccatc agctcatcca gttcattttc tgttactttt
    11281 attttttcca tacacccacg tcctttttta cattataaat gaccttgcgc ttgatggaat
    11341 aaaaagagca tgatcatgct atacacaccc accgaccatg gtttcaagaa aatcacaatc
    11401 tgcataaatt ccctaaaaga taatttcgtc aaaccagcta ctagacagat caagtcgtct
    11461 ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt taatatcttt cgtttttctt
    11521 gtcagcaatt catcaaattt ctgatacttt tttggcgata aaataagctg gacaaaggct
    11581 acgccatagt aacggacaag caaaaatccg attgcttccc caatcactag gccaatacaa
    11641 ctatataaaa gcccaaatcc attgccaaac atcagcatgc ccgctactga ggagatccct
    11701 cccggcaaaa taggaatgat cacttggatc acttgcaaca gaacaaatat catcactgca
    11761 tacttgccaa attgctggat aaacgcttgc aaagacgtgc tcgatcgaaa aatcccatga
    11821 tgataagcat aaagaatcaa cacaaagaaa aaaaacagac caataatggg catgatctga
    11881 agacattttt gtaaaactga tcctttttta gacatctata ttccccacat cttttttcat
    11941 ttattaaaac tgcctcct
//
LOCUS       DACCCR010000079        11845 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCCR010000079 DACCCR010000000
VERSION     DACCCR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.086"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(37..456)
                     /locus_tag="IVK93_002221"
     CDS             complement(37..456)
                     /locus_tag="IVK93_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HAP6387498.1"
                     /translation="MKKIIVATHYRLAEGFKSTLGYIAPNMVEIIAINAYLENTSVEN
                     QVQAALDQFEAEEQILVFTDLLGGSVNQTFAKFLDRYNIQLIAGVNLPILMTLALAIG
                     SNDLSETTIRQAIEESRQQIVYVNDLLQVSAMDDMDE"
     gene            complement(477..1307)
                     /locus_tag="IVK93_002222"
     CDS             complement(477..1307)
                     /locus_tag="IVK93_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6387499.1"
                     /translation="MNNERTKGLTQELNKKDRRNAAWRWSFIGSNNINYGTLQGTGYA
                     WSMAKTLRKIYPNDDDYVEAMRVEYEYFNITPYMGPLVAGADVALQEQQGTESLGAVR
                     SIKTSLMGPLSGIGDSLLWVLYPTIMGSISGYMALEGNPLGAIIWIILNLGLVWMRLK
                     LFDLGYESGIRLITDMADKLNAITESASVMGLTVVGSLIATVVKVYTPIEFTFGEVTM
                     GLQAEVFDRILPALLPVLLTALVYFLMEKKKWGFLKVIFLLLAFSLLGAYFGFLGVAP
                     "
     gene            complement(1300..2088)
                     /locus_tag="IVK93_002223"
     CDS             complement(1300..2088)
                     /locus_tag="IVK93_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6387500.1"
                     /translation="MPEFLQNILIILIPMLTLIDNRGITIINHWPVTVGLLAGLVMGD
                     LQSAMVIAGTFQLMSLGVAALGGSSVPDYGLATVVAIYLNARTGAEIGTSIAVGLPVG
                     ILAINLDIFSRTINSFVSEKSKQYLEERKFSKMQWINFISVFFVAMQAFIPMVILVAF
                     GQVAIETATQWIPEWITNGLNVAGGMLPVVGVAMLMRYMPTKRYIWALLAGFIMAAYM
                     ELPILAVSIVGLALAVFIYNNLTSQKVAVTTENAAYDEGDDFDE"
     gene            complement(2113..2586)
                     /locus_tag="IVK93_002224"
     CDS             complement(2113..2586)
                     /locus_tag="IVK93_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6387501.1"
                     /translation="MGLVLARIDERLVHGIVVTQWASAVKAKRIMVVDDQVAQDEPRK
                     AAMRLSKPAGTGMSLIDTETGITNFKAGKYDSHNVLLVVNNVKTLLDLSEQGIAIPKV
                     NIGIMLDRNDRKKLAKNFAASPEELQQLQQLADKGIPVSYQFAPSDKEEPLDKFI"
     gene            complement(2603..4660)
                     /locus_tag="IVK93_002225"
     CDS             complement(2603..4660)
                     /locus_tag="IVK93_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 31"
                     /protein_id="HAP6387502.1"
                     /translation="MLFKSKEVIVSEQSFVVKDRNQCLKVEGWGRDTVRVTCYPNHKG
                     FDPKQGIEELEKNVLTTATVTETSDQLILTNNRLTATYDGEKLVFYNNGTIILEEYSR
                     KQSQVRRTVGIDTHIPIPDEPTSSLNISPREFTYSGENTYQATLRFEGDPNEKLYGMG
                     GYQEENFNKNGGTYELMQRNSQTSIPFYLSDKQYGFIWNNAAIGEVTFGYNQKIWKSN
                     NTNLIDYLVTVGENPKQIIENYTQMTGRTPRLEPELLGLWQSKLRYQTLDELRTVYEG
                     YNQRGISLSVLVVDYYHWTSDGDFAFDSRYWEGIEDFAEEIKKTGTQLMVSLWPTVTE
                     ESRYYQSYRNNQLLIRSVTNKDSLFAGKALLDFFHPESKTFLRQVLDENYRSKGISLF
                     WADQAEPEMDVYQHKEYLIYEGSLEKYANKYPLHYLQAIQKNPERSNTEPTLIRSAWF
                     NAQKYGALAWSGDIESSFDSLRRQVQVGLSMGISGITWWTSDIGGFHSGNSQSPVFKE
                     LMIRWFQFAVFSPILRMHGDRQPHTQKIGNSGGGVRTSGGPNEIWSFGEEVEGILTRF
                     IHIRTRLKDYIRDVYNESATKGLPIMRSLFIEFPDDPKSWEETTHYLFGSDLLIAPVV
                     TEGATHIPVYLPKGCNWIQVFTKKVYPGGQIYKIAVTLNTIPVFCKEGAPLEHHLADI
                     FGN"
     gene            complement(4710..6767)
                     /locus_tag="IVK93_002226"
     CDS             complement(4710..6767)
                     /locus_tag="IVK93_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HAP6387503.1"
                     /translation="MITIEANRIVKKYDDELLIIEPWGQHSLRVRSFLDQHFVERENA
                     LTELHGYDEKLVETYYKNNQATIVNGKIKAVLDHRDRLTFYNEKDEVLLKEFIRLRAV
                     KHDDGSEDVGTVEFTKDFNSTLKLKSREYRANNDGSNFRTKVRFEPHAQEKIYGMGQY
                     QQNYLDLKNTMLELAQRNSQMSVPFYVSSRNYGFLWNHPGIGKVTFAKNMTEWEMYGT
                     DYIDYWITAGDSPKEITEQYTSVTGRVPEMPKKLLGLWQSKLRYRTPEEVLEVVEGYK
                     QRGIQLSTIAIDYFHWPKQGEYRFDPDFWPEPEKLVKTLKEDYNVEPLISIWPTVQSD
                     AYNYEQYLQGGYLIKVNRGVRLTMQIQGSTVFMDTTNPEARDYVWNLIKKNYKDIGID
                     YYWVDVAEPGYAVYDFENYRYHSGTAVESGNLYPNGYLKMVYDGNKDSNESVVTLVRG
                     AWAGAQKYGALAWSGDIDSSFEAFHNQVNSGLNMGMAGIPWWTTDIGGFHGGNPEDPE
                     FRELMVRWFQYSTFSPILRMHGDRLPHSKPLSNSGGGSMPTGAPNEIWSYGSEVENIM
                     TDYVEIRERMKDYLAESMTEAHELGYPIMRTLFYEFPEDKKSWEIDNTYMLGSDILVA
                     PIMNYKERGREVYLPSGEKWRHLFTGKEYAGGATYYVAAPLEQIPVFIKESASELFES
                     LGK"
     gene            complement(6790..8283)
                     /gene="xylB"
                     /locus_tag="IVK93_002227"
     CDS             complement(6790..8283)
                     /gene="xylB"
                     /locus_tag="IVK93_002227"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAP6387504.1"
                     /translation="MGYVLGLDLGTGALKGVLVDETGVIRHIEACDYPLHSPKGGYNE
                     QNPADWLTACGALFEKFSCNVSDFQAELAGISFSGQMHSLVVTDEAGTVLRPAILWND
                     VRNSAQCKRIMDEFGNEIIAITKNRALEGFTLPKILWIQEHEPAIWEKVRKIFLPKDY
                     LRYYLTGHSHMDYSDAAGTLLMDTTKKEWSKPILEKYNIPEVILPPLVESSACVGTLK
                     PEIKATFGFEKEVRIFTGGADNAVSSLGSGLTDSHTALASIGTSGVFLALEEEDHTRY
                     GGKVHLFNHVLPDTYYAMGVTLAAGHSVSWFKNLLAPEQSYDEFLRNIPNVPIGSNGL
                     LFTPYISGERTPHVDSQIRGSFIGLSNHHTRDDLIRAVLEGVTFSLKDSQQLMTSLKG
                     EEFKRIISVGGGAKNKTWLQMQADIFNTEVVSLDTEEGPGLGAAILAAVGLGWFDSFE
                     SCTNQVIRYGTTVQPIPENVEKYNTVYDLYTKVYGATKDLSHALSIL"
     gene            complement(8302..9609)
                     /gene="xylA"
                     /locus_tag="IVK93_002228"
     CDS             complement(8302..9609)
                     /gene="xylA"
                     /locus_tag="IVK93_002228"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAP6387505.1"
                     /translation="MNYFEAVPKIQYEGKQTKNKFAFRYYNPEEEILGKPMKEHLRFA
                     VAYWHTITADGSDPFGAGTYQRNWEGNNPMETAKNRVEAFFEILEKLGAEYFCFHDVD
                     IAPQGDSLEEFFQNIDTITDLIKEKMDQTGIKLLWNTANMFTHPRFVNGAATTNNADV
                     YAIAAAQVKKGLDISKKLNGQNYVFWGGREGYESLLNTNMDFELDNLARFYKMAIAYT
                     KKIGHQPQFLIEPKPKEPSKHQYDFDAATSMAFIMKYGLQDHFKLNLEANHATLAGHT
                     FEHEINVARSYNALGSLDANQGDLLLGWDTDEFPTDIYSTTLAMYEVLENGGIAPGGI
                     NFDAKVRRTSFEMEDLILAHIVGMDTYARGLRAAAKMKEERFFEEIMEEKYASFTQGI
                     GKKIIDDQETFESLSEYALSLSDVTNQSSHIEYIKSLINDYLV"
     gene            10041..11012
                     /locus_tag="IVK93_002229"
     CDS             10041..11012
                     /locus_tag="IVK93_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6387506.1"
                     /translation="MASVSGGRKPIYLSFNNECALVIAIEIGKDYIHSSLSYINGELV
                     NKLSYHSLLVTKDTLVMLLTKIISDLTSKIPQTPYGILGIGMAIHGIVKDDTVLFTPY
                     SDIDSIPLKDLLAKEIPYPIVLINEANAGALGEYTFKTPSENLVNINIQDGIGAGIVE
                     NGKLFIGANGTAGEIGHTTLYPDGILCPCGNHGCLEHYASTRILLKKLLDSDHKPHPD
                     ISVIVKKWHQGDAHTQKILKHNAKLLSIGINNIVLMYDPEVVIINSEIYRGIPELIDE
                     IKKHLTGKNTKNLAIKNTSLKGNSTLYGCVSIVAQSFLKINNLKFTV"
     gene            complement(11103..11414)
                     /locus_tag="IVK93_002230"
     CDS             complement(11103..11414)
                     /locus_tag="IVK93_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387507.1"
                     /translation="MRNKPITVTLFANAYDNELKTNPHSKLKEGTYSVDNDRISFLTK
                     KGEGLVLNLNIQHIDSIATFNQSVIQIKDHNMTEVKNKIYAFSKKSNDKSYWETLAEI
                     I"
BASE COUNT         3237 a         2622 c         2264 g         3722 t
ORIGIN      
        1 gcaaaaagat tgaacgaatt caatcttttt gcttttttat tcatccatat catccatagc
       61 tgatacttgc aacaaatcgt taacataaac aatttgttgt cgggactctt cgatggcttg
      121 gcgaatggtt gtttcactca aatcgttgct accgattgct aaggctaaag tcattaagat
      181 tggcaaatta acgcccgcaa tcaattggat attgtaacgg tctaaaaatt tggcaaaagt
      241 ctggttaacg gaacctccca acaaatccgt gaaaactaat atttgttctt cggcttcgaa
      301 ttggtctaat gccgcttgga cttggttttc aacagaagtg ttttctaggt acgcattaat
      361 agcaatgatt tccaccatat ttggtgcaat atagcctaac gtactcttga acccttccgc
      421 taagcggtag tgtgtagcta caataatttt tttcacctat ttccctcctc gtatgattaa
      481 ggtgcgactc ctaagaaacc gaagtaggcc ccaagtaaag agaacgcgag cagcaagaag
      541 ataaccttta aaaagcccca ttttttcttt tccatcaaga agtaaaccaa tgcggtcaac
      601 aagaccggta acaatgctgg taagatacgg tcaaagactt ccgcctgcaa gcccatggtt
      661 acttcaccga aagtaaattc tattggcgtg taaactttaa caacagtcgc aattaagctt
      721 cccactaccg tcagtcccat gacggaagct gattctgtga tggcgttgag tttatcggcc
      781 atgtcagtga ttaaacgtat ccccgattcg taccccaaat caaacaattt cagacgcatc
      841 cagactaatc cgaggttcaa tataatccaa atgatagcgc ctaatgggtt accttccaga
      901 gccatgtagc cactgataga tcccatgata gttgggtaca atacccatag taaggaatct
      961 ccgataccgg acaaaggacc catcagggat gttttgattg aacggacagc tcccaaggat
     1021 tctgtccctt gttgctcttg tagcgctacg tcagcaccag cgactaaggg tcccatataa
     1081 ggggtgatgt tgaagtactc atattcaacc ctcattgctt ccacgtaatc atcgtcattt
     1141 ggataaattt tacgcaaggt tttcgccatg gaccaggcat agccggttcc ttgcaaggtt
     1201 ccgtagttaa tgttgttact gccgataaaa gaccagcgcc aagctgcatt acgacgatct
     1261 tttttgttta gttcctgtgt aagtcctttt gttctctcat tattcatcga agtcatctcc
     1321 ctcatcgtat gcagcatttt cagttgttac agccactttt tggctcgtta agttattata
     1381 gataaaaact gccagtgcta atccaacaat tgaaacggcc aagattggta attccatata
     1441 tgctgccatt ataaaaccgg ctaacaaagc ccaaatgtag cgttttgttg gcatatagcg
     1501 cattaacatg gctaccccga cgactggcag cataccccca gcgacgttta aaccatttgt
     1561 aatccattct ggaatccatt gggtggctgt ttcaattgct acttgaccaa aggctacgag
     1621 gataaccatc ggaatgaaag cttgcatcgc gacgaagaag acactgatga agttaatcca
     1681 ttgcatctta ctaaatttcc gttcctccaa atattgttta gatttttcgg aaacgaaaga
     1741 gttgattgtc cgcgaaaaga tgtccaagtt aatagccaaa ataccgactg gtaaaccgac
     1801 cgcgatggaa gttcctattt ctgctcctgt acgagcattc aagtaaatcg caacaacggt
     1861 tgccaaacca taatccggta ccgatgatcc acccaatgcg gctactccaa ggctcattaa
     1921 ttgaaacgtt ccagcgataa ccatggcgga ttgcaaatct cccatcacta aaccagctaa
     1981 aagaccaacc gttactggcc agtggttaat aatggtaatt ccccggttat caataagagt
     2041 aagcataggg attaaaataa ttaagatatt ctgtaaaaat tctggcatta taattcctcc
     2101 aatctcaatt tattaaataa atttgtctaa cggttcttct ttgtcggatg gagcaaattg
     2161 gtatgatacc ggaatgccct tatctgcaag ctgttgcaac tgttgcaatt cttctgggga
     2221 agccgcgaaa ttcttagcta actttttgcg gtcgtttcta tccagcataa tcccgatgtt
     2281 cactttcgga atggcgatgc cttgttcact caagtcgagt aacgttttaa cattattaac
     2341 taccaacaac acgttatgac tgtcatattt cccggcctta aaattggtaa tgccagtttc
     2401 tgtgtcaatc aaggacattc ctgtccctgc tggtttgctt agacgcatag ctgcctttct
     2461 tggttcatct tgggcaactt gatcgtccac taccatgata cgttttgctt ttaccgcact
     2521 ggcccattgt gtcacaacaa ttccgtgaac cagtcgttca tcaattcgag ctaatactaa
     2581 tcccattttt catttctcct tttcaatttc caaatatatc tgctagatgg tgttcgagtg
     2641 gtgctccctc tttacaaaat accggaatgg tattcagtgt tacagcaatt ttgtatatct
     2701 gaccaccagg gtaaactttt ttcgtgaaaa cttgaatcca attacaaccc tttggcaagt
     2761 agactggtat gtgtgttgcg ccttctgtca caaccggagc aatcaacaaa tcgctgccga
     2821 ataaataatg agtggtttct tcccaagatt tgggatcatc tggaaattct atgaacagac
     2881 tacgcataat tggtaaacct tttgtagcgc tctcattata tacatcccta atgtaatcct
     2941 tgagacgcgt tctgatatgg ataaagcgcg tcaatatgcc ttcaacttct tccccgaaag
     3001 accatatttc atttggtcca ccgcttgtcc ttaccccccc tcccgaattc cctatttttt
     3061 gggtatgagg ttggcgatca ccgtgcatcc gtagaatcgg actgaaaaca gcgaactgaa
     3121 accagcgaat catgagttct ttgaaaactg gggactgact gtttccacta tggaaacctc
     3181 cgatatcgct cgtccaccaa gtaatgccac ttatgcccat actaagaccg acttgaactt
     3241 gtcttcttag tgaatcgaat gaactttcga tatcccccga ccaagctaat gccccgtatt
     3301 tctgggcgtt aaaccaggca gaacggatta aggtaggttc tgtgttcgat ctctctggat
     3361 ttttctggat tgcctgtaga taatggagag ggtatttatt ggcatatttt tctaagctac
     3421 cctcgtatat aagatattcc ttgtgttggt acacatccat ttctggttcc gcttggtcag
     3481 cccaaaacaa ggaaattccc ttgctccggt aattttcgtc caagacctga cggaggaatg
     3541 tcttactctc tggatggaag aaatctaaca gagcttttcc ggcaaatagc gaatctttat
     3601 ttgtgaccga cctgatcaag agttgattat tccggtagct ctgataataa cgactctcct
     3661 cggttaccgt tggccataga gataccatca gttgggtgcc agtcttcttg atttcttccg
     3721 caaagtcctc gattccttcc caatagcgag aatcaaatgc gaaatcacca tctgaagtcc
     3781 aatgatagta atcaacaacc aaaacggaaa gagaaatgcc tcgttgattg taaccttcat
     3841 aaaccgttct taattcatcc aacgtctgat aacgcagctt gctttgccat aaccccagta
     3901 attccggttc caaccgaggc gtgcgcccag tcatttgcgt ataattttct atgatttgtt
     3961 tcggattttc tccgacggtc accaagtaat caataagatt cgtattgttg ctcttccata
     4021 ttttctggtt gtagccaaat gttacttccc cgattgccgc attattccag atgaaaccat
     4081 actgcttatc ggagagatag aatggtattg acgtctggga gttccgttgc attaactcgt
     4141 acgttccccc gtttttgtta aagttttctt cttgatatcc gcccatcccg tacaattttt
     4201 cattcggatc accttcaaaa cgaagagttg cttggtaggt gttctcgcca gaataggtga
     4261 attctcgagg tgaaatattt aacgatgagg tcggttcgtc cggaatcgga atgtgggtgt
     4321 caatgcctac agtacgtcgg acttgacttt gcttccgaga gtattcctcc aggattattg
     4381 tcccgttgtt gtaaaagacc aacttttctc catcgtatgt ggctgttaaa cggttgttgg
     4441 tcaagattaa ttggtccgac gtctctgtaa cggttgctgt agtcaagacg ttcttctcca
     4501 attcttctat cccttgtttt gggtcgaacc ctttgtggtt gggataacag gtaacgcgca
     4561 ccgtgtcgcg tccccatcct tccactttca ggcattgatt acggtccttc acgacgaagg
     4621 actgttcgga aacaatcact tcttttgatt taaagagcat agcttctttc ctcccaattg
     4681 acttgtcctt tatctcctct ctttatgggc tatttgccta acgattcaaa aagttcagag
     4741 gcggattctt taataaatac cgggatttgt tcaagaggtg ctgcgacata ataagtcgct
     4801 cccccagcat attctttacc ggtgaataag tgtcgccatt tttcaccgct tggtaagtaa
     4861 acctccctac ctctttcttt gtagttcata atcggagcaa ccaggatgtc agaaccaagc
     4921 atgtaggtat tatcgatttc ccaggatttt ttgtcctctg ggaattcgta gaataatgtc
     4981 cgcatgattg ggtatccgag ttcatgtgct tctgtcattg attctgccaa gtagtctttc
     5041 atccgttctc taatttccac gtagtccgtc atgatgtttt caacttcact gccgtaagac
     5101 caaatttcgt ttggtgcacc tgttggcatc gaaccaccac cagaattgga gagcggttta
     5161 ctatgtggca gacggtctcc atgcatgcgc aaaataggag aaaacgtgct gtactggaac
     5221 cagcgaacca tcaattcgcg gaattccgga tcttccggat taccaccatg gaagcccccg
     5281 atgtcagtcg tccaccatgg aataccggcc atccccatgt tcaatccgga gttgacttga
     5341 ttatgaaagg cttcgaaact ggaatcaata tccccagacc aagccaaagc accatacttc
     5401 tgagcacctg cccaagcacc tcgtaccaat gtgacaacgg attcatttga atccttattg
     5461 ccatcgtaaa ccatcttcaa gtatccgttt ggatacaagt tgccactttc caccgccgtt
     5521 ccggaatggt agcgataatt ttcgaaatca taaacagcat aacctggttc agccacatcc
     5581 acccagtagt aatcaatacc gatgtcttta taatttttct tgattaggtt ccagacataa
     5641 tcacgggctt caggattagt cgtatccatg aaaacagtgc ttccttggat ttgcatcgtc
     5701 aagcgaactc cgcgattcac tttaatcaaa tagccacctt ggagatattg ctcatagtta
     5761 taggcatctg attggacagt tggccaaatc gaaatcaatg gttcgacatt gtaatcttcc
     5821 ttgagtgttt tgaccagttt ttctggttct ggccaaaaat cgggatcaaa tcgatactcc
     5881 ccttgcttcg gccagtggaa atagtcaata gcaatagtcg atagctgaat gcctcgctgt
     5941 ttgtatcctt ctaccacttc taagacttct tcaggagtgc gatagcgtag tttactttgc
     6001 cataatccca gcagtttttt cggcatttca ggaacacgtc ccgtaacgct cgtgtattgc
     6061 tccgtaattt ctttaggtga atctccggct gtaatccaat aatcgatata atccgttccg
     6121 tacatttccc attcggtcat gttctttgca aaggttactt ttcctatacc aggatgattc
     6181 cacaagaaac cgtagttccg gcttgaaacg taaaagggaa ccgacatttg tgaattacgt
     6241 tgagccaatt cgagcattgt gttctttaaa tcaagataat tttgttggta ttgccccatc
     6301 ccatatattt tttcctgggc atgcggttca aatcggactt ttgttcgaaa gttcgacccg
     6361 tcgttatttg cacggtattc acgagacttc aactttaaag tggaattaaa atctttggta
     6421 aattcgactg tcccaacatc ctcactgcca tcgtcatgct taacggccct cagacgaatg
     6481 aattctttta agagaacttc atccttttca ttgtaaaacg tcaaacgatc tcgatgatca
     6541 aggacagctt ttattttacc gtttacaatc gtagcttgat tgtttttgta gtatgtctca
     6601 accagctttt catcataccc atgcagttca gttaatgcat tttcccgctc aacaaagtgt
     6661 tgatccaaaa acgaccggac tcgaagtgaa tgctggcccc atggttcaat aattagtaat
     6721 tcatcatcgt atttttttac gattcgattt gcttcaattg taatcatgat ttcctcctat
     6781 tagaattact tacaatatac taagcgcatg agacaaatct tttgtcgcac cgtacacctt
     6841 cgtatataag tcataaacag tattatattt ttctacgttt tccggaattg gttggacagt
     6901 agtaccatac cgaatgacct ggttggtaca agattcgaaa gaatcgaacc aacctagacc
     6961 gactgcagcc aagattgctg ctcccaaacc cggaccttct tccgtatcca ggctcacgac
     7021 ttccgtgtta aaaatatcag cctgcatctg taaccaagtc ttgtttttag cgccgccacc
     7081 cacactaatg atgcgcttga actcttcgcc ttttaaagaa gtcataagtt gttgagaatc
     7141 cttcaaggaa aatgttaccc cttccaaaac agcccgaatc aggtcatccc gggtatggtg
     7201 attactcaat ccgatgaagg acccacggat ttgactgtca acgtgtggtg tgcgttcccc
     7261 agaaatatac ggggtaaata gtagtccatt actgcctatc ggcacattag gaatgtttct
     7321 taagaattca tcgtatgatt gttccggtgc cagtaaattt ttgaaccatg agacgctatg
     7381 tccagcagcc aatgtgacgc ccatcgcgta gtaggtatct ggcaatacat ggttgaatag
     7441 atggactttt ccaccataac gcgtgtgatc ctcttcttcc agggccaaga acacaccgga
     7501 agtaccgata cttgctaaag cggtatggga atccgtcaat ccagatccca atgaggacac
     7561 agcattgtcg gctccaccgg taaaaattcg tacctctttt tcaaagccaa aggtcgcttt
     7621 gatttctggt ttcaatgttc caacacaagc ggatgattcc actaatgggg gtaagataac
     7681 ttcgggaatg ttatattttt ccagtatagg tttggaccac tctttcttag tcgtatccat
     7741 caataaagta ccggcggcat cggaataatc catgtggctg tggcccgtca ggtaatagcg
     7801 taagtaatcc ttgggtaaaa atattttacg taccttttcc cagatagcgg gctcatgttc
     7861 ctgtatccaa aggatttttg gtaaggtgaa tccttccaga gccctgttct tagtgattgc
     7921 aatgatttca ttcccaaatt catccataat gcgcttacat tgggccgaat ttcgaacatc
     7981 attccatagg atagccggtc gtaagacagt tcccgcttca tctgtaacga ccaaggaatg
     8041 catttgtccg gaaaaactaa ttcctgctag ttcagcctgg aaatcagaga cattgcaaga
     8101 aaatttttcg aacaatgcac cacaggctgt gagccaatca gcgggatttt gttcattata
     8161 gccccctttt gggctatgaa gggggtaatc acaagcttca atgtgccgaa ttaccccggt
     8221 ttcatccacc aacactcctt taagggcccc tgttcctaag tctagcccta atacataacc
     8281 catgttttac accccgactt tctatactaa ataatcatta atcagtgatt tgatgtattc
     8341 gatatgactt gattggtttg tcacatctga aagggaaagt gcgtactcac taagcgattc
     8401 aaaggtttct tggtcatcaa taattttttt accgattcct tgggtgaagc ttgcatattt
     8461 ttcttccata atttcttcga agaaacgttc ttctttcatc ttagcagcgg cacgcaaacc
     8521 acgtgcataa gtatccatcc ctacgatatg tgctaaaatc aaatcttcca tttcgaagga
     8581 agtccgacgt actttagcat cgaagttgat accacctggt gcaatccccc cattttccaa
     8641 gacctcatac attgccaaag tcgtggaata aatatctgta gggaattcgt ctgtatccca
     8701 ccccaacaac aaatcacctt ggttagcatc caacgaaccc aatgcgttgt aactgcgggc
     8761 tacattgatt tcgtgttcga acgtatgtcc tgccaacgta gcatggttgg cttcaaggtt
     8821 aagtttaaag tggtcttgta aaccatattt catgatgaac gccattgaag tagctgcatc
     8881 aaaatcatat tggtgttttg atggttcttt tggttttggt tcaatcagaa attggggttg
     8941 atggccaatt tttttcgtat aggcaatcgc cattttgtag aaacgagcca agttatccaa
     9001 ttcaaaatcc atgttggtat tcaataaact ttcatagcct tctcttccac cccaaaaaac
     9061 ataattttga ccatttaatt ttttgctaat atctaaacct ttttttactt gtgctgcagc
     9121 aatagcgtag acatcagcat tattagttgt tgctgctccg ttgacaaaac gaggatgtgt
     9181 aaacatatta gcagtgttcc aaagtaattt aatgcccgtt tgatccattt tttctttaat
     9241 cagatcggta atggtatcga tgttttggaa aaactcttcc aaggaatcac cttgaggagc
     9301 aatatcgaca tcgtggaaac agaaatattc cgctcccaat ttttctaaaa tttcaaagaa
     9361 agcctccaca cggtttttag ccgtttccat tgggttgttg ccttcccaat ttctttggta
     9421 agtacctgca ccgaatggat cggagccgtc tgccgtaatt gtgtgccagt atgctaccgc
     9481 aaaacggaga tgctctttca tcggcttgcc tagtatttct tcttcgggat tatagtagcg
     9541 aaaagcaaat ttatttttag tctgtttgcc ctcgtattgt atttttggta ccgcttcaaa
     9601 ataattcatt gctaattcca cctttcttag ttagttcgaa actcgtacta acctgatatg
     9661 attatattac ctgggtggga acagcttgtc aagcgctttc gttttttaaa aattaccttg
     9721 gttggttagg tgcgatttat attttttccc tgggacttcc tttagaatcc ttgacaagat
     9781 tgaaattata gtaaatgatt taataaaatc tatttcatat ttgaagtatt tcgaaattat
     9841 cttaattaaa ggagcagact aaattgaatg attacacacc gacacattac aatgagtcga
     9901 ccatcctcga attcatcatt caaaacgccg aggtttctcg atcaaaatta gcgaaattaa
     9961 caaacttaac gaagtcgtct atttccgata tcacaaaaac attgattgaa aaatcactcg
    10021 tctacgaatt aaacactgga atggcctccg tatcaggcgg tagaaaacct atttacttga
    10081 gttttaacaa cgaatgcgcc cttgttattg cgatagagat tgggaaggac tatatccata
    10141 gttctttgtc ctacatcaat ggggagttgg tcaataaatt gagttaccat tccctcttag
    10201 taaccaagga tacacttgtc atgttgttga caaaaataat ctccgattta acttcgaaaa
    10261 taccccagac cccttatggt attttaggaa ttgggatggc aatccatggc atcgtgaaag
    10321 atgatacagt cttgtttacg ccttattcag acattgattc cattcctttg aaagacctac
    10381 ttgccaaaga aattccctac ccaatcgtct taattaatga ggcaaacgcg ggtgctcttg
    10441 gggagtatac gtttaaaacc ccttcggaga atttggttaa catcaatatt caagacggga
    10501 ttggggctgg tatagttgag aatggtaagc tgtttatcgg ggcaaacggc acagcagggg
    10561 aaattggaca cactaccctt taccccgatg gcattctatg tccgtgcggg aatcatgggt
    10621 gtttggagca ttatgcttct acccgtattc tgttgaagaa gctgcttgat tcagatcata
    10681 aaccacatcc ggacatctca gtaatcgtta aaaaatggca ccagggggat gcacataccc
    10741 agaaaatact gaagcataat gcgaaattac tcagcatcgg tattaacaat attgtgctaa
    10801 tgtatgaccc agaggttgtc atcatcaaca gtgaaattta ccgaggcatt cccgaattga
    10861 ttgacgagat taagaagcac ttgactggta aaaacacaaa aaacttagct attaagaata
    10921 catctctaaa aggtaattct acactctatg gctgtgtttc catagttgcc cagtcctttc
    10981 tgaaaatcaa taatctgaaa tttacagtgt aaagtgatag aaaaatatcc tccttaaaac
    11041 ttcctatttt tcgggaggat atgattttat tagggtgtaa gtacttagca gcattttttt
    11101 cactaaatga tttcagctaa cgtttcccaa taacttttat catttgattt tttagaaaaa
    11161 gcataaattt tgttttttac ttccgtcata ttatgatctt ttatttgaat aacgctctga
    11221 ttgaaagtag ctatactgtc aatatgctga atatttaaat ttaacactaa tccttcccct
    11281 ttttttgtta aaaatgaaat ccgatcatta tctacgctat aagttccttc ttttaatttt
    11341 gaatgaggat ttgtttttag ctcgttatca taagcgttag caaataacgt aacggttata
    11401 ggtttatttc tcattttttc gtctctcctt tttggtctag agttttacaa cttttttctt
    11461 ggcgtttgtc aagaagccta tcttttgatc ttatttttta tttttttaat tatcttctgt
    11521 tctgttcata cagttgactc ctttcttatt tagtcctttt ttcaagtatg tccgtctgtc
    11581 gcctgttttg cccttatcat gagggattta gggaagtaaa gggcgagtgt agcgagtgca
    11641 ttttaccctt tacttcccta aagaacttat gatataaacc ggttggcgag agactgactt
    11701 gaaaatagga cgtgaataga agtaataggt tatgaacaaa cgggtaattt tttcaataaa
    11761 aaaacgaaca aaagcagcaa tctttttcga ttgctgcttt tgttcgtttc atctgtttta
    11821 attgcattta tttttgggta ttttg
//
LOCUS       DACCCR010000080        11441 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCCR010000080 DACCCR010000000
VERSION     DACCCR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.087"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            185..1183
                     /locus_tag="IVK93_002231"
     CDS             185..1183
                     /locus_tag="IVK93_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6387508.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IVK93_002232"
     CDS             1295..1804
                     /locus_tag="IVK93_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP6387509.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IVK93_002233"
     CDS             complement(1834..2493)
                     /locus_tag="IVK93_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP6387510.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IVK93_002234"
     CDS             complement(2495..3259)
                     /locus_tag="IVK93_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP6387511.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IVK93_002235"
     CDS             complement(3267..3944)
                     /locus_tag="IVK93_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP6387512.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IVK93_002236"
     CDS             complement(3981..5366)
                     /locus_tag="IVK93_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP6387513.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IVK93_002237"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVK93_002237"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVK93_002238"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVK93_002238"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP6387514.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IVK93_002239"
     CDS             6305..7162
                     /locus_tag="IVK93_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP6387515.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IVK93_002240"
     CDS             complement(7243..7578)
                     /locus_tag="IVK93_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387516.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IVK93_002241"
     CDS             complement(7645..9015)
                     /locus_tag="IVK93_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP6387517.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IVK93_002242"
     CDS             complement(9093..9629)
                     /locus_tag="IVK93_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6387518.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="IVK93_002243"
     CDS             9938..11419
                     /locus_tag="IVK93_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6387519.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3516 a         2301 c         1854 g         3770 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc t
//
LOCUS       DACCCR010000081        11433 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCCR010000081 DACCCR010000000
VERSION     DACCCR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.088"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(24..359)
                     /locus_tag="IVK93_002244"
     CDS             complement(24..359)
                     /locus_tag="IVK93_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6387520.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            645..2525
                     /locus_tag="IVK93_002245"
     CDS             645..2525
                     /locus_tag="IVK93_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP6387521.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2604..2831
                     /locus_tag="IVK93_002246"
     CDS             2604..2831
                     /locus_tag="IVK93_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP6387522.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2836..3378
                     /locus_tag="IVK93_002247"
     CDS             2836..3378
                     /locus_tag="IVK93_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6387523.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3629..4306
                     /locus_tag="IVK93_002248"
     CDS             3629..4306
                     /locus_tag="IVK93_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP6387524.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            4627..5868
                     /gene="sstT"
                     /locus_tag="IVK93_002249"
     CDS             4627..5868
                     /gene="sstT"
                     /locus_tag="IVK93_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP6387525.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            6015..6650
                     /locus_tag="IVK93_002250"
     CDS             6015..6650
                     /locus_tag="IVK93_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP6387526.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6841..7584
                     /gene="truA"
                     /locus_tag="IVK93_002251"
     CDS             6841..7584
                     /gene="truA"
                     /locus_tag="IVK93_002251"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6387527.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            7818..8651
                     /gene="msrA"
                     /locus_tag="IVK93_002252"
     CDS             7818..8651
                     /gene="msrA"
                     /locus_tag="IVK93_002252"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6387528.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            8732..9725
                     /locus_tag="IVK93_002253"
                     /pseudo
     CDS             8732..9725
                     /locus_tag="IVK93_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            10014..10712
                     /locus_tag="IVK93_002254"
     CDS             10014..10712
                     /locus_tag="IVK93_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP6387529.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            10862..11317
                     /locus_tag="IVK93_002255"
                     /pseudo
     CDS             10862..11317
                     /locus_tag="IVK93_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
BASE COUNT         3775 a         1849 c         2385 g         3424 t
ORIGIN      
        1 atactcgttt acataaaaaa agattattta gatgcgataa tatcatagat aaagaaacat
       61 tcatcataag aaacagtgat cccaaattct gtttcaatat ttttgaaggc cttttttaca
      121 tgaaaaattg tttcatgaga aggtcttgtg tcatcttctg atataatttt aagttcatga
      181 ccgtttatca atgtacgctc aattagacac gctgtatgaa tcaaaagttt taaaagaatg
      241 gaatgagtga attttttgtg gaaagtacat tccaattctt ggataaaatc cattaagctt
      301 ttttggatct tattcccagc taataagtag agatatttgt ccactgcttc aactcccata
      361 tcaaaaaacg atatttcttg tgaatgagtg gctgctgccc ttttttgatt gttgttcttc
      421 agtcgtaaga tgacaaagca tcttatcttt gaataaaaaa atagaactac ctaaggaagc
      481 gtttcggcgc ttcctttttc tatatgatcg tttaatgact gtaggaattc agtataccaa
      541 aagaaaaccg cctttttgaa taaacttgat agtggtcaat tttttatgat ggataagtat
      601 ctatacttag tctcgtaaga atgaaaagga aaagagggag aaagatgaaa ttacaaaagt
      661 ggatccaaga gcatcgaaat catatcaccg ccttatcagc tgtgttgatt atttttggtt
      721 ttatcgggaa atttactgga tttgtcttgt tctataacat tgctctagcc atagcttcta
      781 ttattgcggt catacctatt ataatccatg cgtatcaggc agtaaaagtc aaagttatct
      841 caattgattt actcgttact attgctgtgc ttggtgcctt tatgatcgga gaatacaagg
      901 agtcagctat cgttacattc ttgtttttgt ttggtagtta tctggaacaa aaaacattga
      961 ccaaaacgcg ggaatcaatc aaggctttga ccgagatggc cccatcgact gccagcgttg
     1021 tccaagaaga tggaacgacc gaagaagtgg aaatcgatga cgtggaagaa ggcgatatcg
     1081 tcttagtaaa aactggtgga gctattcctg tggacggacg agtcattgac gggaaagggt
     1141 atgcggatga agccgcagtg acgggtgaat cacgacaagt cgtcaaaatc agtggagata
     1201 acgtattttc tggaacgatt ttagcagatg gctacgtgac gatcgaagca actaaggtcg
     1261 gagatgatac gacctttgcc aaaattatag aattagtcga agaagcacaa gataccaaat
     1321 cacatgcgga aaaattcatt gatcaatttt ctcagtatta tactccaacc gttttactta
     1381 ttgcgttgct tgttggtctg atcagtcgtg attttcgttt agccatcact gttttggttc
     1441 ttggttgtcc tggcgcttta gtcattggtg cgcctgtttc gaatgtagca ggaatcggta
     1501 atggagcgaa acggggcatc ttagtcaaag gcggtgaagc aatggatact ttttccaaaa
     1561 tcgatacttt tgtctttgat aaaaccggta cattgaccaa aggaactact gctgtatcca
     1621 ctatcaagaa ctatacagag gatgaagggg acagcttagc tttagtggct cgattagaaa
     1681 cgttatccga tcatcctctg gggcgcgccg tgatccatta tgctcaggag aaagacattg
     1741 attttcaaca attagcagtc aaagataatc agacgatcaa gggacaagga atcacagctg
     1801 agattgatgg tcatacagtt tgtgcgggca acaccaaatt gattgccgct cacaaacttt
     1861 ctctcacacc aaagcaagta caagatttac atcaattaca acaaagaggc agctcagtca
     1921 ttattgtagc aatcgacaaa aaaatcactc aaatcattgg tgtgtcagat gtgattcgac
     1981 cagaagtcgc tgaacaatta gctatgttga aacaagcagg agcaaaacat ttagtgatgc
     2041 tgacaggtga taatcagatg actgctgatt atgtagctga catgttaggc attgatgaag
     2101 tccatgctga attattgcca gatgaaaagg ttcaatttgt gaaaaaatac caagaacaag
     2161 gattgcacgt tgctttcatt ggagatggaa tcaatgattc tccctctttg gctgcggctg
     2221 atatcgggat tgctatggga tcaggaacgg atgtcgctat cgaaacgagt gacgttgtct
     2281 tgatgcaatc tagttttgga tcgttggttc atgcttaccg tttggctaag aaaactgttt
     2341 tgaatacccg tgaaaatgta gtgattgcca ttggtgtcgt cttattttta ctcatcggct
     2401 tatttgccgg gtttatttac atggctagtg ggatgttcgt tcacgaagct agtatcttga
     2461 tcgttatctt taacgcgatg cgtttattgt attttggaca cgaaagcaaa gaagaaaaat
     2521 cgtaaaactt gataagaatc aattttttta tggaagctgt aatctagaat aagactatca
     2581 agaaaaagga aggaagaact acgatgaaaa aaatgattat gcaattagat acactagctt
     2641 gtccaagctg tatgcaaaaa atagaacatg cggtttctca acagcctggg gtaagcaata
     2701 cgaaagtttt gtttaatgcg agcaaaatca aagcaaactt tgatccagat gaagtccacc
     2761 ctgaacaatt agctgcagtt gtggaaaaat taggttatga agtccaatca acaaaagtaa
     2821 aagaggcgta aatcagtgaa tatagtggaa gaaaaatttg ctcaagaaac aaaacaagca
     2881 gatatcgatc atcataaacc aactgctggc gccatggtgg ggcatgtatt ggcaaatttt
     2941 aaattagaag aaatgaaatt gatccaagca caattgtatg ttttgggtac atcttcgcaa
     3001 gaattaagta cccttttttc agaatatgct gatcaagaag cgagatggtt caataaaatc
     3061 tctcatgagt tagtgattga aaatgaaatc gttccgtcta ctttagaaga agtcatgcgt
     3121 tatggaaaaa tttacgaaca tggtcaaaac aaatatttac cagcctcaac aatgttggaa
     3181 aactttgcaa aagattttga tttccaaaat gtctttatca cacgggcaat caaattagct
     3241 gaaaaagaag aaaaatatgg cttgaaacaa ttgcttgttg aacttttaag tttcaataaa
     3301 gaaatcatct accaagtaca aagactccaa ggaaaaactg tccgacaaga tttggatgag
     3361 gaagacgatg atgactaaaa gtctatgttt cttcaaatac aaatataaag cgtgaggtaa
     3421 attggaaaat cgcgaagatt aaaataaaag aagcacccgc tgatgtatta cagacaacat
     3481 cagcgggtgc ttcttagttt ttaaaaggtt taaaagatgg atgagaaaat acaaaaaatc
     3541 aataaaatca cattgcttat ttgatagaat aaagaagtgg gagtaatcga aaggagtttt
     3601 ttaatttaaa aaaaggattt tagtaacaat tggtctagct tttttgttag ctggttgtac
     3661 caacgggaat gaatcgaaaa aagaatcaac aaaagaaaca gcgaaagcaa caattgcaac
     3721 tactacaaca actagcacag agagcaagcc aactgcagca agtacggaaa cagcagatac
     3781 ctctacttcg tcagagtctt ccaaaacgac tgaaacaact caaacatcta cttcaagttc
     3841 ttctgtagag gcttcatctt cagaaagcag cgaacaagtc gaacaaacac tgtggaatgc
     3901 agacaaatcc gcacaattag aggcatttat ggctacatgg ggccaaacaa tgggacaaca
     3961 atacaaaagc tatacgaatc aaatgagtgt ggacttatat ggtcttaaag tccctcaagt
     4021 tattttgaac ggggagtgga agatggctat tggtggagtg cctgtttctg cagaatggtc
     4081 agaaagtggg acaggtcaag cagactatca aatcacagcc gtctattccg atgcagagac
     4141 agaaccatat ctgaaaaagc acgtctatct attcgggttt cagcaaaatc agccaaaagt
     4201 attggtgaca caacaaaatc aggggaatcc cgataattat ctttacttta acgagacagc
     4261 gaataatgaa ttgaaaaatg gctttaatca aatcgtttat gggtaagtaa accagtgaga
     4321 gtgagagaaa gaagagactc agtggaaaat attaattgat tgctatgtct aagaattaag
     4381 atgaaggatg aaatttctag tttcgtcctt catcttagtt acgttttact tggataacta
     4441 taataataac gataaatagt tttctctcga cttctttaga gattgaataa accctccaat
     4501 actaaaattg aatgttgcaa gagcatgaat tgccaattgt attattagaa tttttagaaa
     4561 attgcacctt tagtttcttt agataaaatg atacaatgat tgttaaaatt gtgaaagggt
     4621 ggtcatttgt tagagaagtt tttggatatg tcgttagttc aacgtattgt tgttggtatt
     4681 ttagtcggaa tattgtttgg tatctttttc cccacgtgga catttatttc aatattgggg
     4741 acgttgtttg tcagttgctt gaaggcaatc gcacctattc tggttttttt cttgacgatg
     4801 gcgtcaattg ccaagcataa aattgggaat aagacattcg tgaagcctat tttgatctta
     4861 tatttagtgg gaacattgct ttctgcactt gtagcagtca ttgtaagttc aatttttact
     4921 gttcctatta ctttacaaga aacagtatct gaaaaagcac cgcaaagttt ggaaagtgtt
     4981 ttatctacta tgttgacgaa tgtcacacaa aatcctgttc aatctcttat tgatgcgaat
     5041 taccttggcg ttttattctg ggctgtattg ttgggactag cttttcgagc acgttcagaa
     5101 ccgacaaaag agttggtgga tcagatatct atagcgttgt cacaagtcgt acaagtgatt
     5161 attgcatttg ctccgatagg gattttagga ttggtttatc aatcaattgc tacgactgga
     5221 atcgctggat tggcagagta tctgcagctt ttacttgttt tgatcggtac gatgctgttt
     5281 gtcgcattag ttgtgtaccc attcctcacg tttttgttta ttaaagaaaa tccctatcca
     5341 ttgattttct tttgtttgaa agaaagcgca cttcccgctt tctttacccg aagttctgcc
     5401 gcaaatatcc cgataaacat gatgttggct gaaagattga aattgacaaa agagtcttat
     5461 tccatatcga tcccgttagg tgctacgatc aatatgggcg gtgccgcgat taccatttcc
     5521 ttgatgacgt tgactgctgt acattcttta gggattgaag caccatttgt tttgaaagtc
     5581 cttttatgta ttttgtctgc tttagctgct tgtggagctt caggtgttgc gggcggttca
     5641 ctgttattga ttccattagc atgtagtttg tttggtatct ctaatgatgt cgcgatgcaa
     5701 atcgttggta ttggatttat tatcggtgtt atccaggatt caatggaaac tgccttaaat
     5761 tcgtcaagtg atctgttgtt tactgcaata ggagagctag gcgcgcgtaa aagaagtggg
     5821 gaaaaaatca aattgaaaga ttgtttagat ggattaagtg aacgttgaac gttagatatt
     5881 tagtattttt aaaatgattc gagtttttaa tgagttatat agatgttcgt gtaaatagag
     5941 gtttctgtta aatcaagata aagtcgttta taataacaat taaaagcata ggtaattgtt
     6001 attggaggcg tatcatggat tttttacgta aatttggttt tttcttttta ttattaggcg
     6061 tgctgagtga ttttttgact ccgtacgtat taggattgta ttatcctgaa ttagatcaaa
     6121 tgaagagcgt gatcagtgta tttggagagg tttccagtcc tgtaagaaaa gcgtttctta
     6181 tttggtctgt ggtttcaggg atctttttca tcctgtcatt gcctgcattg tatgctgcat
     6241 ttgtaagtac atctcgtgtg ttagctaccc tagttacagc agccatcgcg ttctatggta
     6301 taggtgattg tatctttaca ggtttgttca gtatcaatac ccatgaatct tcgtggaatg
     6361 tctcgacatg ggtccataat atcggatcag gtttaggtta cagtggtttt ttattgtttc
     6421 cagttcttct tttcttgatc tatcgaaaaa atgggcaaat ggacgtaagc cacttatacc
     6481 ttggactgtc tgtgatcagt ttgctttttg cggcgattta tggactggca agaattcctt
     6541 ctataaattc ttggccatta ctgaataaaa taggtttttg ccaaagaatc agttttttct
     6601 ttaattatgt gcccattgcc tggttgagta tcttgcatct caaacagtaa aacttgagcc
     6661 gtcaaattga acagtcagcg gatacttcat ccggtggctg ttttttgttt ttctgaaata
     6721 tatatttcca cagttttctt gtatagcttc tgtcttagag aagcaaggag ctatattttg
     6781 tgctacaatc ttaaaaaggt agagagaaga ccaaatggaa gacagaggag ccactaaaat
     6841 atgagaaaca tcaagttgac aatcgaatat gacggaaaaa gatattcagg atggcaaaga
     6901 cttggagatg acgataaaac cattcaagga aaaatcgaaa aagtattgca tcagatgaca
     6961 aatgaagaga tagaaattat tggatcagga agaacagatg ctggcaccca tgcgcgagga
     7021 caagtagcaa attttaaaac aaacacagaa attggactgg cagaaatgat cgactttatg
     7081 aatcgttacc ttccgcggga tattgtcatc aaaagaatcg aagaaatgcc tgagcgcttc
     7141 cattcaagat ataatgcggt gggcaaaaaa tacagctatt atgtttggaa caacgtgatt
     7201 ccttcagcat ttgagcgaaa ccacagcttt tatttccctc aagagttgga tatggacaag
     7261 ctgaatgcag cttgcgaaaa attagtgggt acacatgatt tcattggttt ttctgcattg
     7321 aaaaaatcaa aaaaatccac agtgcgtacg attgatgaat tatctatcga acgaaaggga
     7381 gaaatgcttc attttacttt tgtcggagac ggattcttat ataaaatggt gcggattatc
     7441 atggggacat tactagaaat cggcacaggt gccatgccga tagaaacaat cgatgaaata
     7501 tttgaaagca aagtgcggca acatgcagga gaaaccgtcc ctgcacaagg tctgtttttg
     7561 gatgaagttt attatcgctc ataaaaaaga atcatcttta ctcgacttct gagaattgga
     7621 gaacatccct tttttcagag gtcgtttttt agttaaacca aaccatagag tatgcttttt
     7681 aagataaaga tcatcttata tcatctgaag ctgcttatct tctttgaata aatacgattc
     7741 aatcgattat ttcctcactt tatgataaat tacagctaaa gtcactagct tgtcttcttc
     7801 ggaaaggagg agaatctatg gatgaggaac aaatattcat tcaaacactt tataatctga
     7861 ttttgaatcc gaatacacga gattgggaac gaaaagtatt gattcagaca aagaatgaca
     7921 caagagaaaa tatatccgtt aaagaacagt tatcaaaact agaagtaaca ctacgtccat
     7981 tagctatccg aatgaatctg acgcctgatg taatggactt ttatttattg attacggaag
     8041 gatttgataa ggagcaaaaa tatgactttt ccaagcatgc catgcaagat gctgactatc
     8101 aagaacgtgc agtatttgca gggggatgtt tctggtgtat ggtcgagccg tttgaatcga
     8161 aaaaagggat cctatctgtt ttgtcaggat acacgggcgg acatgttgaa aaaccgaatt
     8221 atgatcaagt aagtggtggt tacacaggtc atgtggaagc tgtggagatc atttatgaca
     8281 caagagaaat aagttatagc gagttgctga caatctattg gcaaattact gatcccacag
     8341 atacatttgg acaatttcaa gatagaggga agcaatatcg tccagtgatt ttttatcaag
     8401 acgaacgaca aaaagaatta gcggaacaat caaaacagaa actagacagt tcaggaactt
     8461 tccaccaacc aatcgtcaca aaaatcgaac cagccggtac gttttggcca gcagaaaact
     8521 atcatcagca attttacaaa aaacaaccga aacgctataa aaaaatccag caagcacgta
     8581 atcaatttct gatctatcaa cgagtaaaaa acaaatggca aaaaaatata cgaaaaaatc
     8641 attttgatta actccttttc tttaaaatgt agtaaactaa aaatagaaaa caagaaagcg
     8701 ttttaaaaag atttgatgag gaggagaaaa attgtatatt gcagatagtg cacgttatga
     8761 aaaaatggaa tatcgtaaat ccggtcattc aggtttaaaa ttaccgattt tatctctcct
     8821 ctcggattat ggcaaaattt tggtgattat gatcctatcc ataatcaaag agagatttta
     8881 agaggcgcat tcgacatggg aattacccat ttcgaccttg cgaataatta tggaggccct
     8941 gctggagctg cagagaaaaa ctttgggcgt atttttagag aagattttca agcctaccgt
     9001 gatgaactga ttatttctag caaagcaggt tatcatatgt gggaagggcc atatggggaa
     9061 tggggctctc gtaaatccat catatcaagt tgtgaccaaa gtttccaaag aatggggcta
     9121 gattatgttg acatcttcta tcatcatcgc ccagaccctg atacaccttt ggaagaaaca
     9181 gcagaagcgt tgatgcaact tgtaagacaa ggaaaagcat tatatattgg tatatccaac
     9241 tataatggag aagatacaaa aaaatgactg aaattttaaa aagaaaggaa gcaccgttca
     9301 ttattcatca gatgcgctac aatatgtttt ctcgtgcact actagaagac gatctatctc
     9361 cagtattaga agaagaaggg ctaggggcaa tcacgttcag tccgcttgct caagggttgc
     9421 tgacaaatcg ttatctacat ggtattcctg aagattctag agcacatcgt aaggaaatac
     9481 cattcctttc agaagaacaa gttggatcaa ctctggaaaa aatcaaagca ctgcaaacaa
     9541 tcgccgtatc acgaggacaa tcattagcgc aaatagccct tgcatggaat ttacgacaaa
     9601 aatctgttac aagtgtactg atcggagcaa gtcggttaag tcagttacaa gaaagtgttc
     9661 gaatgatgga taatttagat ttctcaccag aagaagaatt gagaatcgac caaattctag
     9721 aatagaaacg atgtaatcaa actttttatc catatattaa aaaaaacaaa agatgacatc
     9781 aagaatcgaa tgaagatcgt ctgttattga acatcatatt ttttgtcgat caaaataaaa
     9841 atgatcgaag gctaggagtg aattttatcc tagtcttttt tatggtaaat atcttcttcg
     9901 tttgtatgtc tatgaacgga ttaatcgatt ttttatacaa aaaatcatca gatgattcaa
     9961 acgtgttata ataattacga aaaaatacat cagatgaaaa ggaggtatgt ggtttggaag
    10021 aaaataaaac cctagtagaa gtaactactg acaagatcct tagatattta aaggaaatgc
    10081 agttgcaagt aggagataaa ctgccgaatg aatatgaatt gtccgattat ttagaggtgg
    10141 ggcgaagcac ggtgcgagaa gctgtgcggg ctctagcttc caggaatatc cttgaagttc
    10201 ggcaaggagc ggggacattt gtcagtgata agcgagggat tgccaatgac ccactcggtt
    10261 tttctttgat tgaagatcag gatcggatga tacgagactt atttgaactg agatatttat
    10321 tggagcctaa gatggcaggt ttggcagcgg aatatgcaac gaagaatcaa atcgtccaaa
    10381 tgaaaaaatt aacgcaaaag attgaaactt cttttcgaga aggcacacaa gaacatgttc
    10441 aattagacat cgattttcat acgatgattg cagaagctag cggaaacata gccttgctgc
    10501 atattatccc aatcattaat gaatcgattg ctttgtttaa taaaaattat gatatccagc
    10561 atctgaaaat cgagacgatc gatattcata cagagatcgt tcaggccatc gaaaggcgga
    10621 acgtacttgc tgcgtttgac gcaatgactg tccatatggc aataaatcgg agagaattac
    10681 agaaaataat aggagaaaaa gaaactaatt gacagttcat ctgatgtatt ttatgataaa
    10741 tacatcagat gattttttgt tttatatttg gtaaaaaaga ggagtaaata gtttctgttc
    10801 accaaaatgt aagcgctgac taatagacaa aaagagatga cgtcaaggag aagaagcagg
    10861 aatgagggga tctggaatga gttttatgga taaattagcc aaagttttag ggaacgtggc
    10921 aacgagaatc aataatttaa ggtatatcat ggtgataaaa aacgcatttg ctgcattgat
    10981 tccggtaatt attacgggtg cttttggcac attgttttca gcaatggtct ttgactcgga
    11041 aaatggctta gcacagataa gttacttgcg ttttttagaa gaattaaagc ctatcgcatc
    11101 tgccatcagc tatgtcacac taagtttctt gaccatctat gctgtttttc taatagggat
    11161 cgaattagcc aagctgaata aagtggatgg tgtattccct ggtatcatcg cagtgatgtc
    11221 ttatttatct gttaatccaa ctgtttatga attagtgaat gaaaatcaga ctgttatagt
    11281 agaaaacgta ttggctaaac aatatacaga cacgaaagtg gtgttagtat caaatcttag
    11341 acagcttttc gtagagacaa gaataaatta aacttatcta cgagaggatg aatttttatg
    11401 acccgatatg aagaaaattt taaacaaatg att
//
LOCUS       DACCCR010000082        11390 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCCR010000082 DACCCR010000000
VERSION     DACCCR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.089"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1596
                     /locus_tag="IVK93_002256"
     CDS             <1..1596
                     /locus_tag="IVK93_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP6387530.1"
                     /translation="SKGYDLDFLKQVMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNP
                     TLFWLANLMNNPHTVATLDVSTDEKQKVIDQINRALDELNDRSDNERKATDRNKSAEE
                     YLELAEYARKLTSNGEISKQVKVRVHVYGNTIQEVDERAQELIENLKGMDYRAVTYLH
                     KTKRDWQSMFTMFDEQAEWFGNIKGQSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTG
                     GPFVWNATYKNSVRLSTNTLLLGMMGAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIP
                     FLKEYNGIMVRLDGSDGMINPLEIFATRTYDEASSLYDDGSVKDLKINEAASYQTHLD
                     KVVYQVQLVSPQLKGTMKAEFKTYLNRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPE
                     AYPTFKEFLDFLGQLELPGASQEKKNRKEEMESIVESLCETYGMIFDGHSTIRHLDQQ
                     QLVFFDIDSITGLDRGVQQCQMYMAMTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFL
                     AMIDECHNGATCSAVKSCGLQVV"
     gene            2284..4161
                     /gene="ltrA"
                     /locus_tag="IVK93_002257"
     CDS             2284..4161
                     /gene="ltrA"
                     /locus_tag="IVK93_002257"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6387531.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            4292..4669
                     /locus_tag="IVK93_002258"
                     /pseudo
     CDS             4292..4669
                     /locus_tag="IVK93_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            4669..5874
                     /locus_tag="IVK93_002259"
     CDS             4669..5874
                     /locus_tag="IVK93_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6387532.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            5890..6534
                     /locus_tag="IVK93_002260"
     CDS             5890..6534
                     /locus_tag="IVK93_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387533.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            6545..7351
                     /locus_tag="IVK93_002261"
     CDS             6545..7351
                     /locus_tag="IVK93_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387534.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            7373..7603
                     /locus_tag="IVK93_002262"
     CDS             7373..7603
                     /locus_tag="IVK93_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387535.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            8149..8424
                     /locus_tag="IVK93_002263"
     CDS             8149..8424
                     /locus_tag="IVK93_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387536.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            8466..8888
                     /locus_tag="IVK93_002264"
     CDS             8466..8888
                     /locus_tag="IVK93_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387537.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            8908..10500
                     /locus_tag="IVK93_002265"
     CDS             8908..10500
                     /locus_tag="IVK93_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387538.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDQEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYVVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYRNLPEKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKNLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            10512..10847
                     /locus_tag="IVK93_002266"
                     /pseudo
     CDS             10512..10847
                     /locus_tag="IVK93_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10994..11188
                     /locus_tag="IVK93_002267"
     CDS             10994..11188
                     /locus_tag="IVK93_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387539.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYPFV"
BASE COUNT         4264 a         1785 c         2399 g         2942 t
ORIGIN      
        1 tccaaagggt atgatttaga ctttttaaaa caagtgatgc cacaaggtgg attaaatttc
       61 gatgaagagt atgtgaccta tggaaatggt tattcacgat gtgtgacagt ttatcactac
      121 acaaaaaatc ctacgctttt ttggttggcc aatctcatga acaatcctca tacagtggca
      181 acactagatg ttagcacaga tgaaaagcaa aaagtgattg atcaaatcaa tcgtgcctta
      241 gatgaattaa atgatcggtc agataatgaa cgaaaagcga ctgaccgaaa taaaagcgcg
      301 gaagaatacc tagaactagc ggaatatgcc cgaaaattga cttctaatgg ggaaatttct
      361 aaacaagtca aagtgagagt acatgtttac ggaaacacga ttcaagaagt agatgaacga
      421 gcacaggaac tgatcgaaaa tctcaaaggg atggattatc gtgcagtgac ctatctgcac
      481 aaaacgaaaa gagactggca atcgatgttc accatgttcg atgaacaagc tgaatggttt
      541 ggaaatatca aagggcaatc actaccatcg ctaaacattg gcggaggttt cccgttccac
      601 caccaaacgc taaaagatcc aggtggaaca caatatgggg aaaccatgac aggcggcccg
      661 tttgtttgga atgccacgta taaaaatagt gtgcggttgt ctaccaatac cttgcttcta
      721 ggaatgatgg gagctggtaa atcgaccgtt cttaaaatga tcgcggaagc ccatttagct
      781 gcaggagact ttatttgggg ctttgaaaag ggaaaagact ttattccctt tttaaaggaa
      841 tataacggca ttatggtgcg tttagacgga tctgacggga tgatcaatcc tttagaaata
      901 tttgccacac gtacgtatga tgaagcttcc agtttatatg acgatggatc agtcaaagat
      961 ttgaagatca atgaagcagc gtcctatcaa acgcacttag acaaagtggt ctatcaggta
     1021 cagttagtga gtcctcagct aaaaggaacg atgaaagcag aatttaagac ctatttaaat
     1081 cgtttttatg aagagtatgg aacagtacca cgcggtttca cttcctcaaa ctcgcgttca
     1141 aatacggaga cgcaagtcac agggaaagat ccagaagctt atccgacgtt caaagaattt
     1201 ttagactttt taggacaact ggaattacca ggtgcttccc aagagaaaaa gaatcgaaaa
     1261 gaagaaatgg aatccatcgt agaatcctta tgtgagacgt atggcatgat ttttgatggt
     1321 catagtacga ttcgacacct tgaccaacaa cagttggtct tttttgacat tgactctatt
     1381 acaggtttag atcgtggggt acaacaatgt caaatgtaca tggctatgac gttgatttgg
     1441 aatcaggcat tgattcaagg aagaagacaa cgacgtttaa ttaaagaaaa acaaatggaa
     1501 gaacaagact tcaaatgctt cttggcgatg attgatgagt gccataatgg tgcgacctgt
     1561 tctgcagtga aaagctgtgg gttgcaagta gtctaaaaac tattgtaata actcttataa
     1621 cgagaagtca aatgagtggg taacgccaca aagggcttcc actgatactc cgacatgcaa
     1681 caagtagttt atgcagacca acgttgtgtg aagctcggtg aagtcggctg aaggataccg
     1741 taaggtaaaa gaaatagtga ataccacgaa agttctttag aggtaaaggg tgtatccgat
     1801 aagtcgggtg tccaaaaaaa cttaatatgg tgagtatgta caaacgtact gacgaactag
     1861 cgaatgtact gtccgaaagg catagctcct agaaatggga gagacagtga gcaaaaaatc
     1921 actcattgtg ctaacttgtg gttgagtaaa agtcgttgtt atgaaagacc atgctgcgtt
     1981 acaggcgtag gagaagttag ggggcaaaag ctagcatcta agttaaggca tgtggataga
     2041 tataagggaa caggggaagc catcaacatg gagagtgcat actcgatgaa gtggtagtaa
     2101 gaaatatgtg aatgacaaat aacttacttt agtgatggtg aaagtagagt cacgactgat
     2161 gaaggttctg taatggaact ggaggaacag gctcgattcg tctgtaaaag tcatgaaacc
     2221 cgttcaacca tgggattctc gtatgactaa aaaggtcacg agccaattgg agcgtgataa
     2281 accatgacaa aagaaacaag tccaaaagat aggaagttgc ggcatgcaga atattatggc
     2341 atgacggaaa tcttcgataa gatatactcc aaaagtttga agggagataa tttcaagaat
     2401 ctaatgcaga ttatcatttc tgataacaac attgtcctag catatcgtaa tatcaagaga
     2461 aatcacggta gtgtgacaag aggagaagat ggagtaacga ttaaagatat tgaaaatctc
     2521 aaccgtaatg aattcgtaga aattgttagg aagcgattcg cacattataa ccctcggaaa
     2581 gtaaggcgtg tagaaattcc taaatataat ggtaaattaa gaccgttggg tattccatca
     2641 atatgggata gaatcgcgca gcaatgcatc ttacaagtat tagaaccaat atgtgaagca
     2701 aaattcaata atcacagcta tggtttccgc ccaaatagat cagcagaaca cgcaatagca
     2761 gattgtatgg cccgaatgaa tcaaagtcac atggaatatg tggtagatgt agatatcaaa
     2821 ggtttcttcg acgaagtcaa tcatactaaa ctcatgagac aaatctggtc tatgggtata
     2881 cgagacaaac agcttctctt aatcattagg aaaatgttaa gagcacccgt agtacttcca
     2941 aatgggaagt tgatataccc aacaaaagga acaccacaag gcggaatact tagcccatta
     3001 cttgcgaaca tcaatctcaa cgaatttgac tggtggatag caaatcagtg ggaggaaaaa
     3061 gatgccaaag agctaaaaca tcattttagg aaaaatggag cttgggataa aggtaatatc
     3121 tatcacaagc tcagaaagag tacaactcta aaagaaatat acctagtgag atacgcagat
     3181 gattttaaaa ttttctgtag aaattataat gaagcgaaaa aagttttcta tgcaactaaa
     3241 atgtggttac aggagcgtct aaagttacca atatctgaag aaaagtcaaa aatcacaaat
     3301 ctttcaaaag aagaaagtga atttttaggc tttacgatga aattagtgac caaaggtaac
     3361 aaaaaggttg ttaatacgca tataggtaca aaagcacaaa gcatgattaa agtgaaacta
     3421 aaaaaacaaa tcaaacgaat tcaaaaaagt cctaatagca aagatacctt tatctatatt
     3481 ggagtctata acagtatggt tattgggatt cataattact tcaaaattgc gactcattgt
     3541 agtgtagcaa tgcgaaaaat tgcaagagaa ctcagaccaa gttttgaaat acgactacga
     3601 gaacaaggaa tctcaaaaga aggacaatac ggaaagtacg aaggatacaa gccttaccta
     3661 cggtcaggac aaatacgata tataagaggg tttccaatat taccagtagg gtatattcaa
     3721 cacagacatc cgatgtgtaa aaaaagaaat attaataaat acaccgaaga aggtagggaa
     3781 gccattcata agaagcaaca aaccgtagaa gaatggaaga tatcgtggtt gtgtagcaat
     3841 ccagtgctta gtaagagagc aacgattgaa tttgcggaca atcgaatttc aaaattcatc
     3901 gctcaaaagg gaagatgtgc agtaacaggt gaggagctta tcttatctga aatgcattgt
     3961 catcatatta tcccttatca tgaaagcaaa agtgatagct atgaaaacct agtgattgtt
     4021 actgaggagg ttcatcgggt aatacacgct acacaaagtg aaacgattga ggaacttcta
     4081 aaatatctaa aattaaatcc aaaacaaaaa gaaaaactca atgagttgag attaaaagtt
     4141 ggtaacgaag aaatatctta ataaatattt gtaggacggg aaaacagaat gtcttttata
     4201 gacgagggaa cgccgtatgc ggtgaaagtc gcacgtacgg tgtggactgg gggaaaagac
     4261 ggagataata tcaaactctt acctatcagt atcgattaat ccagatttta ttgaaacggt
     4321 aaattatgtc acaaactttc aacgagaaat gcgtaaagta gaagcaatga ctgttttagc
     4381 tacacaatcc ccacaagaaa tggtgccaga aaatatgtct tctgataact tctcgaaact
     4441 gaagaaagtc tttgaatttt cttctacgaa gatctttatg cgtatggatg aatctatttt
     4501 acgtcatata gaaggattag ttggtaaatc aatgacaaac tcagaattag aatccattcc
     4561 tcaacaaaat caaggagacg ctgtgatcaa ttttggctca aaagaaacca ttcgtgttca
     4621 ttttagtccg aacaaacgtc aattaaaatt gtttgatgga ggaaaataat ggaaaaccaa
     4681 aatgaaagtt taatcaagca gtatgttaag cgtcgtgcaa agcgacgctt atttttgtgg
     4741 ttgtttggta cttctgcagg tttgattact attttgatca ctgtgttcgt gaccctattt
     4801 ctaatacttg cggcgggctc gattgataat tcggattcag attcaagcag tggtggcgaa
     4861 gcttttacag gagaatattc ggaaggctta ccgatttata aagaaatcaa aggaagaggc
     4921 cctttttctg atgagattgc gcaatatgcc gtaggtgcag cagtgaaata taaattatta
     4981 ccaagtgtga tcctttcgca atacggttac gaatcagcgt ttggtacatc ggcgtccgca
     5041 agaaatgatc tcaactattt tgggattact tggtttgatg gttgtctgtt tcctaaagga
     5101 actgcacgag gaattggtgg cattgaaggt ggctggtaca tgaagtttcc caactcaaaa
     5161 gctgcatttt cctattacgg ctttatggta gccacacaat caaattttaa tgcttgtgta
     5221 ggaaataaaa gccctggcgc aagcttattg atcttaggac gaggcggtta tgccgctgca
     5281 ggaattacgg aagatagtcc gtattataca ggttgtatgt ccattatcac ttctaataaa
     5341 ttaaccgaat atgatgaatt cgccatcaaa cattggggtg aaggtggcaa taacaacggc
     5401 acgattactg gtgaatggac gaatccattc cctggcagca gtttagataa aagttccttc
     5461 agcggtggtc aattatttgg aacgaatcca ggcggtgaat tccgtccgaa cggcttccat
     5521 gatggattag attttggttc agttgatcat ccaggaagtg agattcatgc ggttcatggc
     5581 ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt tgggcgcgtg tgtgatcgtg
     5641 attaattacg atggcttgaa tatggtgtat caagaatttg caaatagtac aggaaattct
     5701 cgagtgaaag taggcgatca agtgaaagtt ggtcaagtaa ttgggatacg agatacagca
     5761 cacttgcatt taggatttac tcgaatggat tggcgtcaag cacaaggaca tgcattcatt
     5821 gatgatggta catggatcga tccgttacca tttttgaata gcagtaaaaa atagatcagg
     5881 agaaaaaata tggaaaataa aacgttaaaa atcgtcttaa cagctgcaat tagcgttagt
     5941 ttaatcggca atattgcgtt gttttggcag ctgaaagagg aacaagcagc aaatacctat
     6001 aaaattgaag aagtggctaa aaacgcgtct gagaagctca aaaaaaattc ctctgaggca
     6061 caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga aatcgatcaa
     6121 ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa ctgaagcaca aagaaagacg
     6181 gcagaagagt tcggtcaatt ggccattgat aaatcattaa gcagaaatga attaacggaa
     6241 aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat taagtcctac agacgatgat
     6301 caccaacatg atgattacgg aagttactcc tttacattag gtgacgtgca aacttacgct
     6361 caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta tacaatcggc
     6421 aatccgcaat tcaaagatgt caaaccacaa aaaataaaag gtggattaac tgtcactgaa
     6481 aaacaagtag atggtcaatg gaaagtcata gactttagtt atttcactcg ttaggaggaa
     6541 gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa aaacgaaaaa gttatcttca
     6601 atggctggga atctttatga ttctcggctt tttgattctt gctagtccga tttatacgaa
     6661 tcagaaggta gatataccag atgaggtaag cacgaacgcc tttttaaaca cgaaagagct
     6721 gacaatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat ttgcgatcga
     6781 tgatgaagat caaagcttgt taaaagaact ggctaatctt aattatcgcg tagaagtaaa
     6841 gacagctaaa ggggattatc aagcaatacg agccaaagtg atcaaggtgt ctgatgatta
     6901 ttttgcagtg aagctaacgc atgtaccaga agaatatatc gccatgcgtt taaccattat
     6961 tcctgaaaaa attgatccaa aagtggatat gcaagaacca caagatctga tttattacgt
     7021 gcacgaagat aaggttaaag atcaagtgaa agatgaagat tatgaacaac acgcgatcaa
     7081 ttacaaagtg aaaggataca agaaagaaca aaaagcagct caaaaacaaa tggaatcttt
     7141 ccaagcaacc attgatttga atgaagaatt agtgaagaag ctaaaagatc aattgcctta
     7201 tcaagtcgca gatgatcagg aaaatactga aaacaagatt aatagctacc agcaagaaat
     7261 tgaaacatcg aaaacgcaac tgaaagatca agaagagatc attcaaaaac ttcaagaaaa
     7321 aattgatcgt atgacaaaag aaaagattta gaaggacagg tggagtgaaa aaatgaagcg
     7381 aacaaacttg gctgacaaag atactttcgc tgagcagaaa gtattgatta aagcctatct
     7441 caaagttgta aaagctaaag aaatatgtcg agaagtgaat ttatctaaca caacaatatc
     7501 tgagttgaag aatggtcgaa agaatttaga tactttaaaa ttcagatcat ttgaagctct
     7561 atatgtagct gctaaaaaat attttacagc tgataacaaa taaaaaacag gagtatttta
     7621 tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat atcaccttaa ccatttgtta
     7681 atgagatggg agaaaacatt aaccacgatt ctaacgcaaa catcaagcac ataaaacatt
     7741 taacactcct cctttctggc tgaagatttc aaccaaacag gaagacagcg tatcaattat
     7801 agcatacact ttttcttaaa ttcatttttc atatgacaaa atatttcctt ctaatcgaca
     7861 aagttagaag gattttttat tgtctaaata cacaaataac cttaattttt aaagagcata
     7921 aggaaacgtg atatcacttt cttacgaaag gtgctaggta ggctgtgcct accacgtttc
     7981 taagaaagat aaatgatatc aaaattttgg atttgtgtgt agatttgtgg gtaggatgaa
     8041 agcgaatgtg ggcagtaaaa taagcgtttg tgtgtagcaa aatcaaaaaa tgtgtaggga
     8101 tatgtgtagc tacacatatc cctacacata ataaatttgg aggaaaatat ggacttatta
     8161 attagaaata ttgaagaaaa gtacattaat aaaattgaca aacgttgtga agaacttacg
     8221 gtcaagactg gcaaaaaatg gagtcgaaat caatacctaa aagttttgat cgaaaatgat
     8281 tttgatcacg ctcttctgaa ttataaaaaa gaccaatttg atcggttgtt agaaaaattt
     8341 gtggatattc aaacttataa tacaaaaact ttagaagaat atattgcaac aaataatcaa
     8401 ttaattggtt tgttaattga ataataaaaa tagtattgtg aatttataaa aggaggaaat
     8461 caattttggc aaaagcaaca agaaaacatg tatatcttta cgatatgcat atcgaagtac
     8521 ttgatgaaat aatttctact catgaagata tatctaatta tagtgaagca gtcagacatt
     8581 tgtgtatgat gtatgatcca aaaatcgaag aaaaaataaa tccaattcaa attaaattga
     8641 atgcaatggg aaaagaaatt tctattttaa ccgagatggt ttcagagttt gcggatcttc
     8701 atttgaaaga tacagggatt ttaattggta caaattcaac tgtttaccaa gaagcaaaag
     8761 agcgtgtgga agggaaaata aaagcgaatc aaacgaagaa gtattctcct aaaatcaaga
     8821 aatcaataaa tgaatctgtt catgccagag tagacccgtt tgatccaaca aagtttttaa
     8881 aaagataaaa agtagaaggg gaacatgatg aataaatcgc ctgcagtcat tctcacttgt
     8941 caatttacct tgcccaataa aaaatctttt tcaacataca ttgattatat gacaagagaa
     9001 aaagcgctgg aagaaatgga acgacgtacg cctgaagaag aacaggaact gcaacgaatc
     9061 aagaatgctt tagaggattt ttatattcct gaaggagaaa ctttttcgga gagtaagacg
     9121 gatcaagaat tcagcgaaac gtcgaatgaa gctcaatctt tgatgggaaa tggaatcaac
     9181 tttgacaagt tagaggaaca agattttacc aaatatcttt cttatatgac ccgtcactat
     9241 gcgttagaac aaaagaaaga actgacgcaa agggaacaaa aagaatacca acgcttgaac
     9301 caagcaataa aaaaatatca aacagagcca cacaaaaaag cttcaggaaa ccttcctgga
     9361 gttttttcta tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat taagcaaatc
     9421 ttccaaagag gacaacaaaa aggctcaatc atttatcaag atgttgtctc tcatgataat
     9481 tcgtatcttg aaaaaatagg attgtacgat cccaaaacag atactttaga tgaagaaggg
     9541 ttaaaagcag ctggaagaaa aatgatggag acattattcg agaaagagca actgaacgaa
     9601 acaggctact ggatggcaac gattcaccgt aacactaaac atatccatat tcattttgca
     9661 gttgttgaaa aagaaaatac gcgtaaacct tacactgagg ttgaaaatgg tgtgagttat
     9721 gttgtaccga aaggaaagag aaggcaagca acattagacg caatgaaaac caattatgtg
     9781 catgagttag aacgtttctc ttatgaaaag gatcgaatga ttggtatgga gaaacgaaat
     9841 ttattgacac gaaaaagtga tttgcgtaac agcttaacaa aagtggtaaa agagccagta
     9901 cgttatgatc gacacgcaat gaacttatta aatgaagtat atagaaactt acctgaaaaa
     9961 cgttcggaat ggaattatgg ggatgaaaat cgaaccaaac tttcaccata cacaagagag
    10021 aaactcgacg agttaaccca atatgtgtta acaaaagaac ctgaatacca agaatatata
    10081 cagatttcaa aagcattgaa agaagaagca aaaaatcttt atggggaaag caagagagaa
    10141 tcaaaggatg cagagaaaaa tgctctcttt gatttaaaaa agcgcacagg aaatgccatc
    10201 ctaacagagc tgaagagaca ggatagtcaa ataaaaccat taatagaaag tgttgatcag
    10261 atgcaatcta tttacaatga acgatccttt cgaacgctta aacaattgcc aacgaatgaa
    10321 tacaaatata gttacgaaga attcatgaag cgcaggaaac aattccaaca aagagttgtc
    10381 acgcagcgga gtttgcgtca attaaatcga acgattaatg cccatgaaga aaaataccac
    10441 gcaagtaaag catacgaaga gcaacaacga agaattcaaa tggaacaaga aagacagtag
    10501 gaggaagaga aatgaaacga tatttgaatc aatggaaagt catagaagga tcactaaaaa
    10561 aagaacgaat cgaacaactt cccgattgtt tggaaaaaga gcatttgttt cagataagag
    10621 aaatgttgag aagcgaacag attgatccaa attaattttt agtcgttgag tacccagcta
    10681 ctggtgtgta ttgttgtaat catgtgaacg gtgaaaaata ttttatcatt caagaatatg
    10741 aggccaagtt aacaccttat tatactactt gggaaatgaa tgaagaggga atcaataact
    10801 ttccatgtaa aagcataaaa gagtctattt cactcacaga gtgttagtta ttaagtgaat
    10861 aaaagaagta aaagagtcct gaacgtgatt gtccaggact cttttttttc actgaattgt
    10921 tttccgccaa ttcattgagt ggtgcgattt ccgactacat tatatcaaaa gaaagagaag
    10981 ggaggaagca gaaatggctt attcaactat tggagttgtt gttgaaaaaa gcagagacaa
    11041 tctcgttttt gtgacagaaa ttcaaacagg cagagctttt gtagtcacag acaaagctgc
    11101 aaaagcttat caaagtggcg atattttaac gttaaatctg actacgaaga cgttcgtcga
    11161 tgcggcagaa gattatccat ttgtttagtc taatggtgat aagtcaagat gaaatttaag
    11221 attttcttta ttgagtaata tgttttgaag cgcacgaaac tagacccgat caaaatcaaa
    11281 tttttttcgg gttgattcaa ggatttgcgt caataacgga ttgttatttc tctatcacac
    11341 tccttggtgg cgtatagagt tggtgtttac gtagtagcgt atccaccaga
//
LOCUS       DACCCR010000083        11239 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCCR010000083 DACCCR010000000
VERSION     DACCCR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.090"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            84..1130
                     /gene="gmd"
                     /locus_tag="IVK93_002268"
     CDS             84..1130
                     /gene="gmd"
                     /locus_tag="IVK93_002268"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAP6387540.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1210..2223
                     /locus_tag="IVK93_002269"
     CDS             1210..2223
                     /locus_tag="IVK93_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387541.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2260..3204
                     /locus_tag="IVK93_002270"
     CDS             2260..3204
                     /locus_tag="IVK93_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAP6387542.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3272..4585
                     /locus_tag="IVK93_002271"
     CDS             3272..4585
                     /locus_tag="IVK93_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP6387543.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4669..5571
                     /locus_tag="IVK93_002272"
     CDS             4669..5571
                     /locus_tag="IVK93_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP6387544.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5576..7228
                     /locus_tag="IVK93_002273"
     CDS             5576..7228
                     /locus_tag="IVK93_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP6387545.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7252..8118
                     /locus_tag="IVK93_002274"
     CDS             7252..8118
                     /locus_tag="IVK93_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6387546.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8160..8351)
                     /locus_tag="IVK93_002275"
     CDS             complement(8160..8351)
                     /locus_tag="IVK93_002275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387547.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8381..8920
                     /locus_tag="IVK93_002276"
     CDS             8381..8920
                     /locus_tag="IVK93_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387548.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9048..9401
                     /locus_tag="IVK93_002277"
     CDS             9048..9401
                     /locus_tag="IVK93_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387549.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9868..10344
                     /locus_tag="IVK93_002278"
                     /pseudo
     CDS             9868..10344
                     /locus_tag="IVK93_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10344..10538
                     /locus_tag="IVK93_002279"
     CDS             10344..10538
                     /locus_tag="IVK93_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387550.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10544..10829
                     /locus_tag="IVK93_002280"
                     /pseudo
     CDS             10544..10829
                     /locus_tag="IVK93_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10843..11209
                     /locus_tag="IVK93_002281"
                     /pseudo
     CDS             10843..11209
                     /locus_tag="IVK93_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
BASE COUNT         3154 a         2303 c         2855 g         2927 t
ORIGIN      
        1 attatcgtgt attcttgaaa agtattagaa attacgagcc atttatatgg tcttatcact
       61 tacataggaa aggataatgt atcatgaaaa aagcgctgat tactggtatt actggtcagg
      121 acggttccta tctggcagat ttcctgctag agaagggcta cgaagttcac ggtatcactc
      181 gccgtgcttc tatttccaat accgctcgca ttgaccattt gatgggcaag atcacccttc
      241 atgacggtga cctgtccgac tcatcttctc tgattcgtat catcagcatt gttcagcctg
      301 atgaaatcta taacctcgct gcacagagcc atgttcaggt ttcttttgat gttcctgagt
      361 actccggcga tgttgatgct ttgggcgtta tgcgtattct ggaagcttgc cgtattctgg
      421 gcctgacgaa gaagactaag atttatcagg catctacttc tgagctgtat ggcaaggttg
      481 aagaagttcc tcagcgtgaa accactccgt ttcatcctta cagcccttat gctgttgcaa
      541 agcagtatgg cttctggatc actaaggaat atcgtgaagc ttatggcatg ttcgctgtca
      601 acggtattct gttcaaccat gaatccgaac gtcgcggtga gaatttcgtc acccgtaaga
      661 tcaccctcgc tgccggccgc attgcagaag gcctgcagga ccatctggaa ctgggcaaca
      721 tggactccct gcgtgactgg ggctatgcaa aggactatgt tgagtgcatg tggatgattc
      781 tccagcatga gactcccgag gacttcgtga tcgctaccgg cgagcagcac actgttcgtg
      841 acttcaccga gaaggcattc gcagcaaatg gcatgaccat tcgttgggaa ggcactggca
      901 ttgatgaaaa gggctacgat gttgaaaccg gcaagatgct cgtttgtgta aatccacagt
      961 ggttccgtcc gactgacgtt gacaacctgt ggggcgaccc gactaaggca aagaccgttc
     1021 tcggttggaa tccgcagaag acctcttacg aggaactggt taacatcatg gcaaagcatg
     1081 accgtgaatt ggcaaagcgc gaagctgcaa tgaagaacgc agtcaagtaa gctgacattg
     1141 aataaacata tctgtttcta tctttccagt cacggcttcg gacatatcgc gcggaatctg
     1201 cccattatta tggcaacact ctgccgatgc gaagcgaaga ttacactggt ttgcggcgca
     1261 aatcatctgg attttgcaaa gcgcaatttg gagcaggaac tgcccaaaga gctgatagac
     1321 cgcgtgcgct atcgtccaat aaacacagat atcggtctga ttaaccgtga gggcacccta
     1381 ctggtggaca cgggtaagct gaccgagatc tgtgaatatt ttttggcaaa ggttccttct
     1441 ttggcaaaga cagaagccga atggctcaag cgagaagctg tcagcgcagt gctttgcgat
     1501 atgccaatct gggcaatgga tgcctgcgat atggcaggag tacctttcct gtatgtagga
     1561 aacttcactt ggacagagct gtatcgcgaa tttttacccg aacatatttg gaaagaatat
     1621 gccaagcgtt acggtaaaat tcagcacacc atgctttatg cgctaaataa cgaagaaatg
     1681 ttggaatttc tccaaaacag cgaaaaaaca ccgacttccg ttgtggcgcg tccgattcat
     1741 aacgacgaag tgcaacgcat taaatcacag catgaaaaac cgcctgtatt tgtcgcactc
     1801 ggtatgtccg cgcggttcaa acagccaatt gatgtcagtc acgcgccgta tgatttcatt
     1861 acaacgctag gtgttccgct tgtgggagaa aacgtaaccg ttatttctaa cagcaccccc
     1921 aatacacagg actatatagc ggcggcagat tacgttatta caaaggctgg atggggaact
     1981 gttgcggagt gtattctcgc acagcgtccg atggcactgt tcgcccgcga tacagttttg
     2041 gaagatagaa caactatttg taaattgcaa aaactgggtc tggcaatttc cgtcacaccg
     2101 gaaaacctcc accgcgtaga gcgggtgatt gaggacctca aacagctaga cacctcaaat
     2161 tttgatacat tttatgatgc tgcgagcgaa atcgcagaca aattgatttc tctcatgcaa
     2221 tgagagatgc tctaaattta gaaaggagca agcattatta tgatggaaaa agacgcaaaa
     2281 atctacgtag ccggtcatcg tggcatggct ggttccgcaa tcgtgcgcga gctgaaccgt
     2341 caggactaca acaatattat cacccgcacc cataaggagc ttgacctgtg ccgtcaggac
     2401 gctgttgaag catttttcgc acaggaaaag ccggattatg tatttctcgc tgctgcaaag
     2461 gttggcggta ttatcgcaaa ccagaacgct ctggcagact tcatgtatga aaacatgatt
     2521 ctggaaatga acgtcatcaa ctccgcatgg aagaatggct gtaagaagtt acagttcctc
     2581 ggttcttcct gtatctatcc gcgtatggca ccgcagccga tgccggaaag ctgtctgctg
     2641 acaagtgagc tggaaaagac caacgaggct tatgcactgg caaagatttc tggcctgaag
     2701 tactgtgagt ttctaaacaa gcagtacggt actgactata tctctgttat gcctactaac
     2761 ctgtatggac ctaacgacaa ctatcatcct actcacagtc atgtgctccc cgctttgatt
     2821 cgtcgcttcc atgaggctaa ggaagcaggt ctgcctactg ttacctgctg gggtgatggt
     2881 tctcctctgc gcgaattcct atatgttgac gatctggcaa acctgtgtgt gttcctgatg
     2941 aataactata gcggtgacga gactgttaat gcaggtactg gcaaggaact gtctatcaag
     3001 gaactgaccg agatggtagc aaaggttatt ggttacgaag gcgagattct gtgggatacc
     3061 agcaagccga acggtacacc tcgtaagctg ctggatgttt ccaaggcaac caagctggga
     3121 tggacatata agactgagtt ggaagatggc atccgtctgt cctacgagga cttcctgaat
     3181 aacccgatgc gtgctgaacg ctaatttacc gaggttgctg tgatttttga ggattgcagc
     3241 agcctctttc ttataaaaag gagagatcat tatgaatatt gttttactgt ctggcggttc
     3301 tggtaagcgc ctttggccgc tgtctaatga catccgttcc aagcagttca ttaagatttt
     3361 caaaactgaa gatggatatg aatccatggt tcagcgtgtc taccgccaga ttaagaaggt
     3421 agataacgac gcaactgtta cgattgcaac ctctaagaca caggtttctg ctatccataa
     3481 ccaacttggt gaggatgtgg gtgtatctgt agagccttgt cgccgcgata ccttcccggc
     3541 aatcgcattg gctaccgctt atctggtaga tgtgatgggc attgatccag aagagtccgt
     3601 agttgtttgc ccggttgacc cttacgtaga agatgactat tttgaggccc tgaaatctct
     3661 gtccgatcag gctgacaaag gcgaagctaa tctggtgctc atgggtattg aaccgactta
     3721 tccttccgag aagtacggct acatcattcc tgagagcgca gagcagactg ctttcgtaaa
     3781 taccttcaag gaaaaacctt ctgaggctgt ggcaaaggaa tatatcgcac agggtgcact
     3841 gtggaacggt ggtgtgtttg tctacaagct gaagtatgtc atggagcgtg cacatcagct
     3901 gattgacttt gtagattatc aggatctgtt caacaaatac gatactctga ctaagatttc
     3961 cttcgactat gccgttgttg agcatgagga caagattcag gtacagcgtt ttgctggtat
     4021 gtggaaggat ctggggactt ggaatactct gaccgaaggt atggaggaat ccattatcgg
     4081 taaaggtgtt atgaatgaga attgcgatgg cgtacacatc ctgaacgaac tggatgttcc
     4141 tgtgttggct atgggtctgc atgatgtggt catctctgca tctcgggaag gtattctggt
     4201 ttccgataag gaacagtcca gctatattaa gcctttcgtt gacaagttcg agcagcagat
     4261 catgttcgca gagaagtctt ggggtagtta taaggttatg gatgtagaag cagagagcat
     4321 gaccattaag gttactctga atgctagtca ctccatgaat tatcacagcc acaagaaccg
     4381 tgacgaggtg tgggtggttc tggccggtac tggtaagacc atcgtggatg gatatgagca
     4441 ggttgttaag gctggcgatg tgatcaccat gagtgctggc tgtcggcata ccattatggc
     4501 agatactgag ctgaagctga ttgaagtaca gcttggcaag gatatcaatg ttaaagataa
     4561 acagaaattt gagttagagt attaaaaaac ggaggagggc tcgttataca gaaaccgttt
     4621 ttattgaagc cttccgcaaa ggattatctg tgggggggaa ccgcctgaat gatgaatttg
     4681 caaaaggaat tgagatgtct cctcttgcgg aaacttggaa tgctctacgc acccggatgg
     4741 accgagcttt gttgtgtctg gtcagagtat caggggatga ccttgacgga ggtgttgcaa
     4801 gaacacccag agtttctggg aacgcatccg cagaccaaag gagaattgcc gatcctgatc
     4861 aaattcattg atgcgaaaaa ggatttgtct gttcaggtac atcccacaga tgaatatgca
     4921 attgaaaaag aaaacgggca gcttggaaag acagagatgt ggtatgtact ggatgccagc
     4981 ttaatctatg ggctgcagaa aataacagaa gaagctgtga tccgtgaagc gattgctcag
     5041 ggaactgttg agcgttatct tcagaaggtt ccggtcaaaa agaatgatgt gttctttatt
     5101 gaggcgggaa cgattcatgc gattggtgcg ggtgcactaa ttgcagaaat tcaggaaaat
     5161 tccaatctga cttatcgtct ctacgattat gaccgtgttg acaaaaatgg taaaaagaga
     5221 gaacttcatg tagacaaagc gctagaagta gcgaacttga aagggttgtc agaaccgaaa
     5281 cagccgcttc gtgttctaaa atatcgtgct ggtgtagctg cggaattgct ttgcagatgc
     5341 aagcatttcg aggtctctcg tatgattgtc aacactgaga gaagacagac ggtacactat
     5401 cgtgcggatt cagtcagctt ccgtgtgttg ctgtgtgtca atggctgtgg taccgttcga
     5461 tttgataatg aatcgttgga catctataag ggggactgcc tttttgtgcc ggccgattct
     5521 actgaaatca gcattcacgg gtcgattcag tttctggatg tgagaggata ataatatgaa
     5581 ttataaagta gaatatgagc gttggctgaa tcatgccaat gcagatatta tagaagaact
     5641 gcgttcctat tctgagaagg acattgagga ctctttctat aaaaatttgt ctttcggtac
     5701 tggtggtctg cgtggtacca tcggtgcagg cactaatcgc atgaacgtac acaccgtagg
     5761 taaggcaagc caaggtctgg cagactatct gaataagaac tatgaagccg cttccgtggt
     5821 ggttggctat gattcccgta tcaaatctga tgtgtttgca aagactgccg cagacatctt
     5881 tgctgcaaat ggtattcagg tgcacttgtg gccagaactg cttcctgttc ctacagtatc
     5941 ttttgcgact cgttatctga aggcagatgc cggtgttatg attacggcat ctcataaccc
     6001 ttctaaatat aacggctaca aggtatacgg tgcagacggc tgtcagatta ctaccgaggc
     6061 tgctactgca attctggcag aaatcgaaaa gctggacatc tttgctgatg taaagatgga
     6121 tggcgatgct tctaagattg catccattca gtcggaagtt ctgactgctt tcatcgaaga
     6181 agtgaagggg cagtccgtcc tgtttggcga aaaagtggac aagaatgttg ctatcgtgta
     6241 ttctcctctg aatggtaccg gtctggtgcc tgttacccgc accctggcag agatgggata
     6301 cagcaatgtg accgttgtgg aagaacagcg tctgccggac ggtaatttcc cgacttgtcc
     6361 ttatccgaac cctgaaatta aggaagctat gtctctgggt atggagtatg ccaagaagta
     6421 caatgcggat ctgctgttgg ccactgaccc tgactgcgac cgtgttggta tcgcagttaa
     6481 gagcgcacat ggtgactatg ttctgttgtc tggtaatgag actggtctgc tacttctgga
     6541 ctacatctgt tctcagagaa ttaagcatgg aaaaatgcct gctgaccctg tgatggtcaa
     6601 gaccatcgtc accatggatt tgggtgaacg tatcgcatcc aactatggtg ttcgtaccat
     6661 cgatgtgctg accggcttta agtttatcgg tgagcagatt ggtatgttgg aaaaggctgg
     6721 taaggctgac agctatatct tcggctttga ggaatcttac ggctatctgt ccggtagcta
     6781 tgtccgtgac aaggacggtg ttaacgctgc ttatctaatc tgtgaaatgt tctcttacta
     6841 caagactcag ggtatcagcc tgctggataa gctgaatgag atttatacaa agtatggcta
     6901 ttgtttgaac accctgcatt ccttcgagtt tgacggtgcg gcaggtttta ccaagatgca
     6961 ggaaattatg gttgaattcc acaagggtct cgatataatt ggtggtaaga aggttattga
     7021 aactttagac tacagtaagg gcctgaatgg tctgccgaag tctgatgttt tgaagtttat
     7081 gctggaagat aactgctctg ttgtggttcg tccttctggt actgagccga agctgaagac
     7141 ctatatcagc gtcagcgcag agaacaagga agccgccgaa gctatggaag ctattatggc
     7201 tgctgaactg gcggaaatgt tcgagtaaga aaagaaagga gaaatcttgt aatggaggaa
     7261 ctgttttcta acgctttgaa atcggtgaag tcaaatcgtg tgctgtacac ggcatcgttc
     7321 tttgcaagat cctctctttt gtatttgcag gaaattggac agctgactgc tctgaaggca
     7381 catacatcta ggcgtacagg attgtcatct tacctttgtt tcctggtaga agttggttca
     7441 ggcacattgg agtataaggg gcagaaatat acgcttcaag taggagatat ggtgtttatt
     7501 gactgtttga agccgtatgt ccatgagtca tcagaagatg atttgtggac tttgcggtgg
     7561 tgccatttta atggcattgg tatgacggaa gtctacaaaa aataccttga acgaggtggt
     7621 caaccggtct ttaaaacatc aaaaacagat aagtatgctg acttgctgag gagccttcat
     7681 atggtggcag agggacagga gtatgtccgg gatatgaaaa tcaatgttct actcagtgaa
     7741 ctgctgatgc atttgatgga agattcttgg aatcctgaca aagtgccgaa tttctctaca
     7801 aaaaaaatgg aattgcagaa cataaaacaa tatctggatg accattacac ggagaagatt
     7861 acgctggatc agttgagtgc agagtttttt atcaataaac agtacttagc taagattttt
     7921 cgtgaaactt acggtacaac tgttgtcggt tatctgaatc agaaacgaat cacacaggcc
     7981 aagcacttgc tacgctttac aaatctaagt atggaagaga ttgcaacaaa aattggcata
     8041 acagatgcaa actatttttc cagaactttt ttcaaggtgg aaggtgttcg tcccagcgaa
     8101 ttccgcaaga tgtggtaata aaaagccggc ggtgttgagc cgccggtcag tacctgtcgt
     8161 taacccgcat tcaaagcttt aatctcagct ttgatttcag cacgaacacg atagtaatct
     8221 tcagtttcga tttcggtatc cagccgagtt gctgtctttc cgcagtcagg acaacgctct
     8281 ggctgttcgt cggcagggaa gctgtagtgg caggaatcac aggtgtaaat gtgatcgtca
     8341 cgtataatca tcacctacca atccagtgct tagatttttc atgcaattac tattatatcg
     8401 aattggtgat tccacaaaaa tccgcttctt gataaatgta cgattcagag tgatatatag
     8461 tactgtactg ccaaagggaa taccacaacg gcagaaatgg atgattcaca tggaagaaat
     8521 tatgcagatg attttaggga acgcaaaatt ccagctcgga caggtggttt cgacccctgc
     8581 tgtattggaa actttcaacc ccctgcagac cgcaaacggc attcccgaag ccttgagcaa
     8641 cctttggttt agacaggaaa ttagtattgc ggagatcgag gacgcagttc tggatgccaa
     8701 gaacggcaag gagctagcaa acagcctcaa ccgcatcaag cttttccgca agttcaccct
     8761 cgaccgagag ctggacggca tggtcagact gaagggcgcg gacgtctgga agaacatcag
     8821 ctacctcaag gtctacaccg ccacgccgcc taagaaaccg acccccccaa ggttccagtc
     8881 gaacggctgg agccttttct tctctcataa atcggcataa tgtatacaaa tacagctcca
     8941 aatgattgtg tagtatatgc ctgtggattg acttgatata gtcgagagac tatggcaata
     9001 tacacacaac caaaggggaa aatcccaaaa acaaggagga aacgctaatg gcaatgagcg
     9061 aaaaggcaaa gaaatatttg aaggagatca agggtgcaaa gaccatcccg gagctgaagg
     9121 atgtggagat cgcaatcaag cgagacggta tccttgcttg ggcagagttc accaagttga
     9181 acgaggcggt ggaagagaag aaggtcgccc ttcgcaagaa aaagcaggag accagccttc
     9241 aggagatcct tttctgggcc tacaaaaagg aatcagacaa gctgctgaag atgatggacg
     9301 agggagcaga caaggatgcc atccagatgc aggtccagcg ttacgacagc atcggccaga
     9361 tcattgcaga ggcagacctc gaagatgagt atgaggtttg agaatacagc ctgaaccact
     9421 aagaaccaaa taccgaaagg gacttctgaa acagacagag gtccctttct tttgctcact
     9481 ttggcggtca attatgaacc aaaaaccagt cgaatgattg tgtacattat ggctttcaaa
     9541 cgacttgata ataatcactt ttagagtgat atatgtacta caaaaaacaa aagactggag
     9601 aaaacaacag atgagcgaat acagaatcat ttatatcagc gacgaggacg gcggcgaggt
     9661 ggaaggatgt ggctacacgg ctacgatggt tatgatgcgg tcagaaactt cttggaattt
     9721 gcctgcggca tcttgaaaat cctgcgggtg cgattcatca gaagagtgga ctgaaataca
     9781 agttagcaga tagagaagag agtcaggttt cgacttggct ctgttctctg tctactggca
     9841 gaaatacatt gaagggaggt gacgactgtg gcaaccagag gaagaaaatc aaccccgaca
     9901 gccatgaaga agctggaggg aaatccgggc aatcgcccac tcaatgcaaa agagtcaacg
     9961 ccggaaaaga aggcaccgac ctgtccgaag tggctggaac cagaggcaaa gaaggaatgg
    10021 aagcgtctgg caaagcagat ggaggccatc ggaatcttga cagatgtgga tatggctgcc
    10081 tttgccggtt agtgccaagc ctacgcccgc tggaaggaag ccgaggaatt cattacccag
    10141 cacggtatca tcgtgaaaac cctatccggc tattggcagc aggtaccaca ggtgtttatt
    10201 gcccagacct atctgaaaat tatgaaccga ttttccgagc agttcggtct gacgccttcg
    10261 tccagaagcc gtatcattgc cggtgatggc ggtggtgtcg gagcagaaga tgcgatggag
    10321 gctctgttgg gaggtggtag ctgatggcag aaaccagacc aaagggctat cccaaattaa
    10381 aggaatacac accaaaccga ttcatgcttt cggaatgtca ctacgacaaa gccagagcgg
    10441 atcgggcggt gaactttatc ggacagctcc gacacaccaa aggcaaatgg gcaggcaacc
    10501 gcttctggct cttgccttgg caggaacaga tcatctagtg taatctcgct taactgtatt
    10561 aacggacgta gcgactttat taagagtgtt catggtagga gcattagttg caacaatgat
    10621 tgatattaaa tttttatggt accaacgatt ggcgatgtga cattgaatat gcaaaactat
    10681 atcgatatga tattatctaa aatcttactc gctgggatcg tcgaggtgtt tattggttgt
    10741 taggaaagaa aaatatgacc caactcgagc gatttttatc gtattacttg tgtgtggctt
    10801 tatctgcatt gtgtgtgatt tcgaaataag ggagagccaa aaatgagtag aaaattgata
    10861 ttgattagtc acggaaaatt ttgtgaggaa ttgaaaaaaa gtacaaaaat gattatggga
    10921 cctcaagaga atatctacac gttaccttta ttaccagaag atggcactga aacatacaga
    10981 caaaagctct tactgtcata gattcgttaa atgattttgt ggtactatgc gatcttttag
    11041 gcggtacacc tactaatatc atttctaaac tgataatgga aggacaacag attaaacttt
    11101 atgtcggatt aatcttccta tggtcattga atttatcaac tcgcaagtga ttggttcaga
    11161 gcccgagttt gttcgtgcag gatgtgaaag cattgtatct gtcaactaat taattagtgg
    11221 aatagtagaa gatgaagag
//
LOCUS       DACCCR010000084        10782 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCCR010000084 DACCCR010000000
VERSION     DACCCR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.091"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            27..3116
                     /locus_tag="IVK93_002282"
     CDS             27..3116
                     /locus_tag="IVK93_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6387551.1"
                     /translation="MSLFYLKEKLEARIEELGKYRFSKIVELSDWLVVEDETKKAKYP
                     PKDFSSAATFSVGDTWKGRDFYLWVKKEIEMPDVDDLYLYLNFGHTGGGYNSGFESLM
                     FINGKPYQGVDSNHKEVKIADEFRGKKVEVALKLWSGLEGGGPEQIQVHQFKQAELSQ
                     MMVPVDELFYYADAINKTVKELGQDNPIQYKLSHVLNHAINQINWIEPGSQTFYTSVA
                     KAVSELSEGIAAIPKDEMITVTAVGHTHIDVAWLWRLKHTREKAARSFSTVLRLMEEY
                     PEYIFLQTQPQLYKYIKEDYPEIYAKIKERVAEGRWEADGSMWLEADCNIPSGESLTR
                     QILHGAKFIKEEFNQDIHYLWLPDVFGYSWALPQILTKSGIDTFMTTKISWNQFNRMP
                     HDTFKWKGMDGSEVLTHFITTPEPGKENSWESNWYYTYNGELEPETVLGVYNAYRDKN
                     INDELLISYGFGDGGGGVNRDMLEKRRKLDKIPGLPKVQTGKAVDYFDRLQETFKNTD
                     EYVHTWDGELYLEYHRGTYTSQAYVKKANRQSELLLRQLEILFSLMEMKGQEAYPKVE
                     IFNMWETVLRNQFHDIIPGSSIKEVYQDHRIEMEGLFKEAQSLLAPLQSVGDSQTVCN
                     FANWQRSILAELPFLLDNQVYVDEAGEVIETYANGEVLYGLISNVPALGQKMIHVTEK
                     QEMAQIPTVAVVDSNQIETDFYLIQWNKSGQLTSVFDKELKREVLSGTGNVLQLFEDK
                     PMNFDAWDIDIYYQEKFQELAAQEIIVKPANALYQCVEFIYRFGESTIKQEMRLYPHT
                     RRIDFVTDIDWQERQQLLKAKFDVAVRATYATYDIQYGNVRRANNWNTSWEMAKFETV
                     AHQWMDMSQRDFGVSILNDSKYGCDVKDQTMRLSLLKGAIHPDPTADMGQHHFTYSLY
                     PHRGDFIEGKTIQEAWEINEKPLVLNGSIGTLPQITTTQDATIVIDTIKKAEDGNGWI
                     IRAHEYAGTDQTVDLTIEGLTWWRETDLLERNIGEVKDKQPSLTFAPYEIKTIRFGK"
     gene            3085..4452
                     /locus_tag="IVK93_002283"
     CDS             3085..4452
                     /locus_tag="IVK93_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HAP6387552.1"
                     /translation="MKLKQFVLGNEGRALEITAEFCQKLKSNYEGTPKYKLAELGVVR
                     NGVVESAENVSAKSQNPFVFSIEVPTLKIPQQYHSGRCWLFAIVNLLNQQISQKLEIA
                     EFELSQNYLYFYDKLEKANFFYKNIIETADLELGSRELDFLLSKPQEDGGQWDMLVSL
                     IEKYGIVPSYSMPETENSRSSADMNKYLNAKLRLDAKKLRQMVAEDCSQKDIDEVTSQ
                     MLAEVYRMLAICIGAPPEKIDMKYRDDAGNYHAGLNLTPKEFANKYLLMNLKDYIPII
                     HAPSKNKVYNQTYTVAMMGNIIEGQPIKYLNVDMATLKALTIKQLKDHEAVWFGSDIG
                     QYTDRKLGIMATDIYAVDNLFDVDFTSNKQDRLEYCQSLLTHAMVISGVDLQNDQPTI
                     WKVQNSWGEEIGYKGFFMMTDAWLEEYVYQVVINKKYLTKEQLAKYQEEPVILPPWDP
                     MGSLA"
     gene            4527..5345
                     /gene="iolB"
                     /locus_tag="IVK93_002284"
     CDS             4527..5345
                     /gene="iolB"
                     /locus_tag="IVK93_002284"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP6387553.1"
                     /translation="MNLLQKPILFPVNEKVQLIQYITGENSVLEHVELSVYDVEAGGE
                     VSYHEAALESCVVVLSGQVSVAILNGETYPKLGTRTSVFDKIPTHSVYIGKNRSYKVT
                     AETKARILIAASKTDQSLPDRLIKPEDVLLESRGAYQNKRYVQTILSDQDTISDKLLV
                     VEVYTEGGNFSSYPPHKHDQNRLPEESFLEETYYHEINPPQGFVFQRIYSDDRLLDQT
                     MSCSNQDVVIVPKGYHPVGVPDGYTSYYLNIMAGPTKMWRFYDDPDHEWIKNRS"
     gene            5395..6372
                     /gene="iolC"
                     /locus_tag="IVK93_002285"
     CDS             5395..6372
                     /gene="iolC"
                     /locus_tag="IVK93_002285"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP6387554.1"
                     /translation="MKKFAITALGRACIDLNSVEINQPMEETKTFKKYVGGSPANIAI
                     GLAKLGETVGFIGKLSNDQHGRFIKDFMSKAGVDSSQMIFDQEGHKSGLAFTEIKSPS
                     ECSILMYREDVADLYLTPDEVSEEYIEASEMLLISGTALAQSPSREAALLAVEYAHKH
                     DVKVIFELDYRPYTWGSTAETAVYYTLLAQKAHIVIGTRDEFDVLENSVGGNNQQTVE
                     RLFQYTPELILIKHGVDGSFAYEKDGTITRGYAYKAQVLKTFGAGDSYAAAFLFALMQ
                     GKMIENALQYASASSALVVSRHSSSEAMPSVSEIEAVIAEQSWGEKVHA"
     gene            6365..8281
                     /gene="iolD"
                     /locus_tag="IVK93_002286"
     CDS             6365..8281
                     /gene="iolD"
                     /locus_tag="IVK93_002286"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP6387555.1"
                     /translation="MPKTLRLTTAQALVKYLNQQFLIVDDQKLPFVKGIFHIFGHGNV
                     LGLGQALSQNPGHLEIYQGKNEQGMGQAAVSFAKEKLRRQIFAVTTSVGPGAANLVTV
                     AGTALANNLPVLLLPGDTFASRQPDPVLQQVEHFHGQGITTNDALKPLSRYWDRIERP
                     EQLMSALTRAFEVLTDPVTAGPVTICLPQDVEGEAYDYPEDFFNEKVHYFDRRLATDR
                     ELSEAVKLIERSLKPVIIVGGGAKYSEAGSALKRFSEKHKIPLVETQAGKSTVVADFP
                     LNFGGVGVTGGAPANRLVKEADLVIGLGTRYTDFTTASKTAFDKDQTTFLNINVSRMQ
                     SYKMNGVSVVADAKTALLQIESELDDLYQTQYGDLLTSVTRDWQNERERLANLNYHDA
                     DFEMEIKNHFGAEKLANYAQQLTTELTQTAALIKINQSLPLDSIVVGAAGSLPGDMQR
                     LWKAKTVNSYNMEYGYSTMGYEVAGAYGAKIAHPDKEVFAFVGDGSFHMLHSELLSSI
                     QYGKKINVLLFDNSGYGCINNLQMGNGMPSFGTELTDYHEEIMTVDFAKIAEAYGAVA
                     YNVGNMSELEKSLAEIAESDRSTLIHIKVLPKTMTDGYDSWWNTGIAEVTADGSTEKV
                     LAEKEEKLAGAFQY"
     gene            8303..9457
                     /locus_tag="IVK93_002287"
     CDS             8303..9457
                     /locus_tag="IVK93_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP6387556.1"
                     /translation="MYQLTLPQKIIYGDQAIKETGKIAGNLGLKTLIITDKLIVSLNL
                     LEGVLASLKEENLTWKIFDGVDREPTDQIVAEALAFYQQEDYDSIIAVGGGSCIDTAK
                     AVAVLINNSGSYAEFASGQLIAQNPLPLIAIPTTAGTGSEVTDVTVITDTQKDIKYMM
                     KQAAFLSYAAIVDPQMSRTSPASVTAATGLDALCHALESYISKKAQPITRLFSLSAIK
                     LIMENLPIAYSEPENLIARENLAQAAMEAGIAFSNASVTLIHGMSRPVGALYHIPHGI
                     SNAILLEAVIDYTKPVIISELAGIAKYLNPEKEGTDQELAECFFQELQDLIEKLAVPN
                     YASFGIEKAAFIASLDKMANDAIASGSPNNNPLVPTIEEIKMIYLASWQE"
     gene            9480..9911
                     /locus_tag="IVK93_002288"
     CDS             9480..9911
                     /locus_tag="IVK93_002288"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6387557.1"
                     /translation="MEIKTDLNSEDSSQLAEFLLMVLDQQQEHFFPDYQTIDKSVALA
                     AYSGTELVGGLLGKISDQSLNVSLLAIDPKHQKDGLGSQLIEEVEKIAAKNQVINLLL
                     STRSYQALAFYQKNNFEIYGELKDMPFRGVDTYYLVKRLMA"
     gene            9949..>10782
                     /locus_tag="IVK93_002289"
     CDS             9949..>10782
                     /locus_tag="IVK93_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP6387558.1"
                     /translation="MTYKEIPVSVQQFMEKITTLCGNDHPQWAANINAAFANTLLTTV
                     KRHEDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKDNPDLADMISGLVKKQFFYINLD
                     PYANAFNEEANNAGHQDDETQMNPWVWERKYEIDSLCYPIQLAYLLYQNTGRTDQFSE
                     DFIRGIEKVLTVFETEQNHQDSPYTFQRFDDRPEDTLVNHGRETTVAPTGMTWSGFRP
                     SDDACHYGYLVPSNMFAVVILDYLVEIFQDILKETTLIDRIIKLKKRSMMVSKNLVRF
                     LI"
BASE COUNT         3390 a         1886 c         2441 g         3065 t
ORIGIN      
        1 aaatgaaaag ggtggaggaa tagttcatga gtttgtttta cttgaaagaa aaattagaag
       61 caagaatcga agagttagga aaatatcgct tttcaaaaat agtagagttg tctgattggc
      121 ttgttgtaga agatgagacc aaaaaagcaa aatatccacc caaagatttt tcaagtgctg
      181 ctactttttc agtaggggat acttggaaag gccgcgattt ttatttgtgg gttaaaaaag
      241 aaatcgagat gccagatgtt gacgatttat atttgtattt gaattttggt cataccggtg
      301 gtggttacaa ctccggcttt gaatcattga tgttcattaa tggaaaaccc tatcaaggcg
      361 tggattccaa tcataaggaa gttaaaatcg ccgatgaatt ccgtggtaaa aaagtcgagg
      421 tagcattgaa gctttggtca ggtttggaag gtggcggacc ggaacaaatc caagtccatc
      481 aatttaagca agcggagcta tctcaaatga tggtgccagt tgatgaacta ttttattacg
      541 ctgatgctat caataagacc gtgaaagaat taggtcaaga taacccgatt caatacaagt
      601 tgtcccatgt tttaaatcat gctatcaacc agattaattg gatcgaacct ggaagccaaa
      661 cattttatac ttcggtagcg aaggctgtat ccgaattatc agaggggatt gccgcaatcc
      721 ctaaagatga aatgatcact gtgacagcag tcgggcatac ccatattgac gtagcgtggt
      781 tatggcgatt aaaacatacc cgcgaaaaag ctgcccgtag cttctcaact gttttacgtt
      841 tgatggagga gtatccggag tatattttct tacaaacaca accgcaatta tacaaatata
      901 tcaaagaaga ctatccagaa atttatgcaa aaatcaaaga acgagtagct gaaggtcgtt
      961 gggaagcgga tggaagcatg tggttggagg cggattgtaa cattccttct ggtgaatctt
     1021 tgacccgcca gattttacat ggtgcgaaat ttattaaaga agaattcaat caagacattc
     1081 actatttgtg gttgccggac gtttttggct attcttgggc attgcctcag attttgacta
     1141 aaagtggcat tgatactttt atgacgacaa agattagttg gaatcagttt aatcggatgc
     1201 ctcatgatac cttcaaatgg aaagggatgg atggctcaga agtcttgacc cattttatta
     1261 ccacgccaga accaggcaaa gagaattcat gggaatccaa ttggtactat acctataatg
     1321 gtgagttaga acctgaaact gttttaggcg tttacaatgc ttatcgagat aaaaacatta
     1381 atgatgagtt gttgatttct tatggatttg gcgatggcgg aggcggcgtt aatcgtgata
     1441 tgttggaaaa acggcggaaa ttagacaaaa ttcctggctt accgaaagtt caaaccggta
     1501 aagcagtgga ttacttcgat cggctgcaag agacctttaa aaatactgat gaatatgttc
     1561 atacctggga tggcgaattg tatttagaat atcatcgagg cacctatacc tctcaagctt
     1621 atgtcaaaaa agcgaaccgc caaagtgaat tattgttgcg ccagttagaa atcctctttt
     1681 cattaatgga aatgaaggga caagaggcct atcccaaagt tgaaatcttt aatatgtggg
     1741 aaacggtttt gcgtaatcaa ttccacgata ttatcccggg atcttcaatt aaagaggttt
     1801 atcaagatca tcggattgaa atggaaggac tttttaaaga agcccaaagt ttattagcac
     1861 cattgcagtc agtaggtgac agccaaacag tttgtaactt tgcaaactgg cagcgttcaa
     1921 ttttagctga attaccattt cttttggata atcaggttta tgttgatgaa gctggtgagg
     1981 taattgaaac atacgctaat ggtgaagtgc tttatggatt aatttcaaat gttccagctc
     2041 ttggtcaaaa aatgattcat gtcactgaga agcaggaaat ggcgcagatt cctacagtag
     2101 ctgttgtgga ttcaaatcaa attgagacag acttttattt gatccagtgg aataaaagcg
     2161 gacaattgac ttcggtattc gataaggagt taaaacgaga agttttaagc ggaaccggga
     2221 atgtcctgca attatttgaa gataaaccga tgaattttga tgcttgggat atcgatattt
     2281 attatcaaga aaaattccaa gaactagcag cacaagaaat tatcgtcaaa cccgctaatg
     2341 ctttatatca atgtgtcgaa tttatttatc gttttggtga atcaaccatt aaacaagaga
     2401 tgagattata tcctcatact cgtcgcattg attttgtgac agatattgat tggcaagagc
     2461 ggcaacagct gctaaaagct aaattcgatg ttgctgtcag agcaacttac gcaacttatg
     2521 atattcagta cgggaatgtt cgtcgtgcca ataactggaa tacgagctgg gaaatggcta
     2581 aattcgagac tgtcgcccat cagtggatgg atatgtcaca acgtgatttt ggggtcagca
     2641 tcttaaatga ttccaaatat ggttgcgacg ttaaggacca aacaatgcgt ttgagtttgt
     2701 tgaaaggagc aattcatcct gatccaacgg cggatatggg ccaacaccat tttacctaca
     2761 gtctgtatcc acatcgtggc gactttattg aaggaaagac cattcaagaa gcttgggaaa
     2821 tcaatgaaaa accgcttgtt ttaaatggta gtattggcac tttaccgcaa attaccacaa
     2881 ctcaggatgc gactatcgta attgatacga ttaaaaaagc cgaagatggt aatggttgga
     2941 ttatccgggc ccatgaatat gccgggactg atcagacggt tgacctgaca attgaaggtt
     3001 taacttggtg gcgtgagacg gacctgttgg aaagaaatat tggcgaagta aaagataaac
     3061 aaccgagttt gacatttgca ccatatgaaa ttaaaacaat tcgttttggg aaatgaggga
     3121 agagcattgg aaataactgc agaattttgt caaaagttaa aaagtaacta cgaaggaacc
     3181 cccaaatata aactagcaga attaggtgtt gttcgaaatg gtgttgttga atcggcagaa
     3241 aatgtctcag ctaaaagtca gaatcccttt gtgttttcaa tcgaagtacc gaccttaaaa
     3301 attcctcagc aatatcattc gggacgctgt tggctgtttg cgattgtcaa tttactgaat
     3361 cagcaaatca gtcagaagtt ggaaatcgct gaatttgaac tttcgcaaaa ttatctatat
     3421 ttttatgaca agttagagaa agccaacttt ttctataaga atattattga gacggctgat
     3481 ttagagttgg gcagtcgtga attagatttt ctactatcta agccccaaga ggatggtggt
     3541 caatgggaca tgctagtttc attgattgaa aagtatggga ttgttccatc ttattcgatg
     3601 ccggaaaccg aaaatagtcg cagttcagct gacatgaaca agtatctaaa tgccaagttg
     3661 cgacttgatg caaaaaaact gcgtcaaatg gtggctgagg attgttcaca aaaagacatt
     3721 gatgaagtga cttcccaaat gctcgcggaa gtttaccgaa tgttggcaat ctgtatcggg
     3781 gctccgccgg aaaagattga tatgaagtat cgtgatgatg ctggaaatta tcacgctgga
     3841 ttaaatctaa caccaaaaga atttgccaat aagtatttgt taatgaattt aaaggattat
     3901 attccgatca ttcatgcacc ttccaaaaac aaagtgtaca accagaccta cacagtagcg
     3961 atgatgggaa atattatcga aggacaaccg attaaatatc ttaatgtaga tatggcaacg
     4021 ttaaaagcat tgacaatcaa gcaactgaaa gatcatgaag ccgtctggtt tggtagtgat
     4081 atcggccaat atactgaccg aaagctaggg attatggcca cagacatcta tgctgttgat
     4141 aatttattcg atgtcgattt tacttcaaat aaacaggacc gtttagaata ctgtcaaagt
     4201 cttttgactc atgccatggt aatcagcgga gtagatttgc aaaatgatca gccgacgatt
     4261 tggaaagtcc agaatagctg gggcgaagaa atcggttata aaggcttttt catgatgacc
     4321 gatgcttggc tagaggaata tgtctatcag gtggtgatta acaaaaaata tttaacaaaa
     4381 gagcaactgg caaagtatca agaggaacca gttattttac caccctggga tccgatgggt
     4441 tctttagcat aataaaaaaa ccgcggctag tcataacgag atgattgttt gtgaccggcc
     4501 cccgatttta gtaaagaggg aaaataatga atcttttaca aaaaccaatt ttatttccag
     4561 tgaacgaaaa agttcagttg attcaatata taaccggaga gaatagcgtc cttgagcatg
     4621 ttgaattaag tgtttacgat gtcgaagctg gcggtgaggt tagttaccat gaagctgctc
     4681 ttgaaagttg cgttgttgtt ctttccggcc aagtgtccgt agcgatttta aatggagaaa
     4741 cctaccctaa gcttggaacg agaacttcag tttttgataa aataccgact cacagtgttt
     4801 atattggtaa aaatcgttca tacaaagtta cggcagaaac gaaagcgcgt attttgattg
     4861 ctgcttctaa aacggatcaa agtctaccag atagattgat caagcctgag gatgtcttat
     4921 tggaatcacg gggtgcttac caaaataaac gctatgtgca gacgatcttg tctgatcaag
     4981 atacaatcag cgataaactt ttagtcgtgg aagtttacac agaaggtggt aatttttcta
     5041 gctatccacc tcataaacac gatcaaaacc gcctaccgga agagtctttc ttagaagaaa
     5101 cttattatca tgaaatcaat ccaccacaag ggtttgtttt tcaacggatt tattccgatg
     5161 atcgtctttt agatcaaacc atgtcctgtt ccaatcaaga cgtggtaatc gttccaaaag
     5221 gctatcatcc tgttggcgta ccagatgggt acacttctta ttatctaaat atcatggccg
     5281 gtccaacgaa aatgtggcgt ttctatgatg atcctgatca cgaatggatc aagaaccgat
     5341 cgtaagagtt tttcagaaaa ataaaacgtg tcatgagaga ggaagaaaat taaaatgaag
     5401 aaatttgcta ttacagcttt agggagagcc tgtattgatt tgaactcagt agaaattaac
     5461 caaccaatgg aagaaacaaa aacatttaaa aaatatgttg ggggatctcc ggcaaatatt
     5521 gcgattggtt tggccaagtt aggggaaaca gtaggtttca tcggaaaact ttctaatgat
     5581 cagcacggtc gatttattaa agacttcatg tcaaaagcgg gtgttgattc aagtcagatg
     5641 atttttgatc aggaaggaca taaatccgga ctggctttta ccgaaattaa gagtccatca
     5701 gaatgcagta ttttgatgta tcgtgaagat gtggctgact tatatctaac ccctgatgaa
     5761 gtttcggaag agtacattga agccagcgaa atgctattga tttcaggaac agctttagcc
     5821 caaagtccgt caagagaagc agcgttattg gcggtggaat atgcccacaa acatgatgta
     5881 aaagtgattt ttgaattgga ttatcgtcct tatacgtggg gatcaactgc tgaaacagcc
     5941 gtttactaca ctttgctagc acaaaaagcc cacattgtga ttggtacccg cgatgaattt
     6001 gatgtgttag aaaatagtgt tgggggtaac aaccaacaaa ctgttgaaag acttttccaa
     6061 tatacgcctg aactgatcct gattaaacac ggggtcgatg gctcatttgc ttatgaaaaa
     6121 gatggcacga ttacgcgagg atatgcctat aaagcacagg ttttgaaaac ctttggggca
     6181 ggtgattctt atgcggccgc tttcttgttt gctttgatgc aaggaaaaat gattgagaat
     6241 gctttacagt acgctagtgc ctcgtcagcg ctagtagtta gtcgtcatag ttcttcggaa
     6301 gctatgccaa gtgtgtccga aattgaggca gtcattgcag aacaaagctg gggggagaaa
     6361 gttcatgcct aaaacgctac gcttaaccac ggcccaggca ttggttaagt atttgaatca
     6421 acagtttttg attgtagacg atcaaaagct accctttgtg aaaggtattt tccatatctt
     6481 tggccacgga aatgtcttag gattgggaca agctttgtca caaaatcctg gtcatttgga
     6541 aatctatcaa ggaaaaaatg aacagggaat gggccaggcg gctgtttcct ttgcaaaaga
     6601 aaaactgcgc cgtcaaattt ttgcagtaac gacttcagtg ggaccgggtg ctgcgaatct
     6661 tgttacagta gctggcactg ccttggctaa taatttgcca gtattattat tgccgggaga
     6721 tacttttgca agccgtcaac cagatccagt cctgcagcaa gtcgaacatt ttcacggtca
     6781 aggaatcacg acaaatgatg cattgaagcc actttctcgt tattgggatc ggattgagcg
     6841 cccagaacaa ttgatgagcg cattaacccg ggcgtttgaa gttttgactg atcctgtgac
     6901 cgcgggtccc gttacaattt gcttacccca agatgtcgaa ggggaggctt acgattaccc
     6961 agaggatttt ttcaatgaaa aagttcatta ttttgatcgc cgcctagcta cagatcgtga
     7021 attatcagag gcggttaagt taattgaaag aagtctgaaa ccagtgatta tcgtgggtgg
     7081 cggggcgaag tattcagaag ccggatcagc tcttaaacgt ttttcggaaa agcataagat
     7141 tcctttagtg gaaacccagg caggaaaatc aacggtggtt gctgattttc cattgaattt
     7201 tggtggtgtc ggtgtgaccg gtggtgcacc tgccaatcgt cttgtgaaag aagctgatct
     7261 agtgattggg ttgggaacac gctacaccga ttttacgaca gcctcaaaaa cggcttttga
     7321 taaggatcaa acgacttttt taaatatcaa tgtttcgcgg atgcaaagct acaaaatgaa
     7381 cggtgtttca gtggtagctg atgccaaaac ggccctattg caaattgaat ctgagcttga
     7441 tgatttatat caaacgcaat atggtgacct gttaactagt gttactaggg attggcagaa
     7501 tgaacgggag cgtctggcaa atttgaacta ccatgatgct gattttgaga tggagatcaa
     7561 aaatcatttt ggcgcagaaa agttagccaa ttatgcgcaa caactaacaa cagaattgac
     7621 tcaaaccgcg gctttaatca aaatcaatca aagtttgccg ctggatagta tcgtggttgg
     7681 ggcggctggc tctttgcccg gtgatatgca acgtctgtgg aaagcgaaga cagttaactc
     7741 ttataatatg gagtatggct attctacgat gggatatgag gtagcgggtg cttatggtgc
     7801 caagattgcg catccagata aagaagtttt tgcttttgtt ggagatggta gttttcatat
     7861 gttgcattcg gagctacttt ccagtattca atatgggaaa aaaatcaatg tcttactttt
     7921 tgataactca ggctatggat gtattaataa tttacaaatg ggcaatggga tgccatcttt
     7981 tggaacggaa ctgacagatt atcatgagga gatcatgacg gttgattttg caaaaatagc
     8041 cgaagcttat ggggcggttg cttataatgt cggtaatatg tctgaacttg agaaatctct
     8101 agctgaaata gctgagtctg accggagtac attaatccat attaaagttt tacctaaaac
     8161 tatgacggat ggatacgatt catggtggaa taccgggatt gctgaagtta ctgcagatgg
     8221 ttctactgaa aaagttttag ctgaaaaaga agaaaaacta gcaggggcct ttcaatactg
     8281 agaagcccag aaaggtgtca aaatgtatca actaactttg ccgcaaaaaa tcatttacgg
     8341 cgatcaagca atcaaggaaa caggaaaaat cgctggtaac cttggtttga aaactttgat
     8401 tattacagat aaactaatcg tctctttgaa ccttttggaa ggtgtgcttg ccagcctaaa
     8461 agaggaaaat ctgacctgga aaatttttga tggcgttgac cgcgagccaa cggaccagat
     8521 tgtagctgaa gcattagctt tttaccaaca ggaagattat gattcgatta ttgcggtggg
     8581 cggtggcagc tgtatcgaca ctgctaaagc tgtggctgtg ttaatcaata atagtgggtc
     8641 ctatgctgaa tttgctagtg gccaactgat tgcccaaaat ccactgcctc taattgcaat
     8701 acctacgact gccgggactg gctccgaagt gacagatgtg accgtaatca cagatacgca
     8761 aaaagatatt aaatatatga tgaaacaggc tgctttttta tcctatgcag cgattgttga
     8821 tccacaaatg agtcgtacaa gtccagcatc agttacagca gcaaccggac tagatgcatt
     8881 atgtcatgct ttagaaagtt atatttctaa aaaagcccag cctattacaa ggctcttttc
     8941 actttcagca ataaagttga ttatggaaaa tttaccgatt gcctatagcg agccggaaaa
     9001 cttaatagcc cgtgaaaact tggcgcaggc agcaatggaa gcgggaattg ccttttcaaa
     9061 tgcttcggtt accttgattc atgggatgtc aagacctgtt ggcgctctgt atcatatccc
     9121 tcatggcatt tccaatgcga ttttattaga ggcggtgatt gattacacaa aacctgtaat
     9181 catctcggag ttggctggta ttgcgaaata tcttaatcct gaaaaagaag gtacggatca
     9241 ggagttggca gagtgcttct ttcaagaatt gcaggacttg attgagaaat tagctgttcc
     9301 caattacgca agttttggta ttgaaaaggc agcttttatt gcatctttgg ataaaatggc
     9361 aaatgatgca attgccagtg gtagccccaa taacaatcct ttggtaccaa caattgagga
     9421 aataaaaatg atttatttag ctagttggca agaatagtca aagaaaagta ggtagaaaaa
     9481 tggagataaa aacagatctt aattcggaag attctagtca gttggctgag tttcttttaa
     9541 tggtcttgga tcaacagcag gaacattttt tccctgatta tcaaacaatc gataaatctg
     9601 tggccttggc tgcttattca ggaactgaat tggtaggcgg tctgctaggg aaaatcagcg
     9661 atcaaagctt gaacgtttcg ttattagcca ttgacccgaa acatcaaaag gatggtctag
     9721 gaagtcagtt gatcgaagaa gtcgaaaaaa ttgcggcaaa aaaccaagtc attaatcttc
     9781 tactttcgac gagaagttat caagcgctgg cgttttacca aaaaaataat tttgaaatct
     9841 atggcgaact caaagacatg ccgtttcgtg gcgttgatac gtattactta gtcaaacgtt
     9901 tgatggcata acgtttaaag ttccaataaa gaataaagag gcacaaaaat gacctacaaa
     9961 gaaataccag tctctgttca acaatttatg gaaaaaatca cgactctttg tggtaatgac
    10021 cacccacaat gggcagcaaa cattaatgct gcctttgcca atactctttt aacaacggtt
    10081 aagcgacatg aagatggcac aacttttttg ttgactggcg atattcccgc aatgtggttg
    10141 cgggattcaa ctgcccaagt tcggccttat ttagtcatcg ccaaagataa tccagatttg
    10201 gcggatatga ttagtggttt agtaaaaaaa caattcttct atattaactt ggatccttat
    10261 gcaaatgctt ttaatgaaga ggccaacaat gcggggcatc aggatgacga aacacaaatg
    10321 aatccttggg tctgggaacg aaagtatgaa attgattctc tttgctatcc aatccagctg
    10381 gcttatttgt tatatcagaa tactggccgt acagatcaat ttagtgaaga ctttattagg
    10441 gggatcgaga aggtcctgac ggtttttgaa acggaacaga accatcaaga ctcaccctac
    10501 acttttcaac ggtttgatga tcgaccggaa gatacgttag tcaatcacgg acgggaaacg
    10561 acagttgcac caacgggtat gacttggtca ggttttcgtc caagtgatga tgcttgccat
    10621 tatggttatc ttgttccgtc aaatatgttt gcggtggtca tccttgatta tttagtagag
    10681 attttccaag atattttgaa ggagacgaca ctgattgacc gtattatcaa gttaaaaaag
    10741 cgatcaatga tggtatccaa aaatttggta agatttttaa ta
//
LOCUS       DACCCR010000085        10512 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCCR010000085 DACCCR010000000
VERSION     DACCCR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.092"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(136..2289)
                     /locus_tag="IVK93_002290"
     CDS             complement(136..2289)
                     /locus_tag="IVK93_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP6387559.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2583..3431)
                     /locus_tag="IVK93_002291"
     CDS             complement(2583..3431)
                     /locus_tag="IVK93_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6387560.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IVK93_002292"
     CDS             complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IVK93_002292"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP6387561.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5932..6111)
                     /locus_tag="IVK93_002293"
     CDS             complement(5932..6111)
                     /locus_tag="IVK93_002293"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP6387562.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IVK93_002294"
     CDS             complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IVK93_002294"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6387563.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8259..>10512
                     /locus_tag="IVK93_002295"
     CDS             8259..>10512
                     /locus_tag="IVK93_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6387564.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDI"
BASE COUNT         3005 a         2250 c         1882 g         3375 t
ORIGIN      
        1 cttttttgcc gagtaaacgg tgttcaaaag ccagtttctc ggtatgaagt caaatttaag
       61 caaaaatgtg agaagctatt ttcacaaaat ttttcttcat actcgaaact aagcggcttt
      121 ttcacgacct cttttttatt ttgccatgaa catatcataa tacgcgtcca ttatttcttt
      181 agcaatcgtc aagttcatgt ggtcttgttc gtctaataag ttcggcaaga caacactgat
      241 ggcaatttcc ggattgtctg ttggtccgta tgccacgata ttactattga ctgtcgtaat
      301 atctggatga ccttccgcaa ccgtttctgc agtccctgtt tttgcggaaa ggtccatttt
      361 cgcactggct aatggtttgg ctgctgtgta aggatcggta ccatggacaa cttggtggaa
      421 tccttcacgg aggatcgcca tattttctgc agaaatattg accgtatttt ctactgttgt
      481 gccaatttct ttcttcactt cacccaagtt tccattttcg tcattgccat agatcgcttt
      541 gactaggtgc ggctgtactc gtttcccgcc attagcgact gttgaagcat attgagctaa
      601 ttggattggt gtatatgtgt caaactgtcc aaatgccaaa tcggtaaagt tacctggagt
      661 ataccattct ttgccattat ccgcattgaa cttcttcata taatcaacag aacgtgagat
      721 cccaggagat tcattcggca aatcgatccc agtcgttgta ccaagaccaa actctttgaa
      781 agagtctctc aattgctgat aagctttagc ttcttcatct aaactaggta gtcccattcc
      841 aggtgtatag tctagtccta acatctttaa cgccactttg atcatatacg tattagaaga
      901 caattccaat gctttgactg catcaagagc aacttgtccg cttcgattaa agacagagct
      961 tttttctgaa gcaccttgca gacgaatcgg ctcatcaatc aaaacttgat ttccgctgat
     1021 cgcgttactt gcccagcctg ctgtcaaagt accagccttg actacagacc ctggagcaaa
     1081 ggcgctagta atcgtgccta aagcattctc ggtgatttct ttggaacctt gttcatgact
     1141 gaaaccagtc attgctagca cttctcctgt ctgaggattc atagccactg cataagcacc
     1201 aggcgaatac tgtgctttgc cgttgttgat caatgtttga taattccttt tcaaaatttc
     1261 ttctactttt gattggaatt ctgcattcat agataacatt aaattagaac ctttttcccc
     1321 agagaagatt tctttttgat tcgaaacatt tccttcatta tcaagggaaa cttcatattg
     1381 ggttttggtt ccttgcagga catcttcata ttgtttttcc aaataacttt gtcctacacg
     1441 gtcattccga gaataacctt ttgctaggta tttctctgct tcatctgctg gaagtccttg
     1501 cttttcagtc gtcacactgc caagaatact tttcagtgaa cttgctgcat tatattctcg
     1561 tgtccagtct gtgccagtcg aaactcctgg aagttcagaa gcgcgttctg ccacaacagc
     1621 taactcttga tcagtaacac cagagttttt gatatagaca gtgtttaatt catatgcact
     1681 attcatccgt ttaaagatgg tagcaatttt taattgctcc tcgtttaaat taatctcatc
     1741 agctgtgact ttattgacca tcgcactata ttgttcgctg ttccctaatt gcttttcttt
     1801 tgccgaaagt ctttttgtgg cttctttcaa atgtttggga tcagccagcc aaaaatcttt
     1861 tttatctcta tctgtcagat tttcatctac cggaacatcg atcagcgagt tcagcttcgt
     1921 tgcgatttca agcaaatctt tagccgtaac ttgtgttcct ctcgtatatg tgatggcctg
     1981 attcgcttta ttcgtaacta aagcttttcc tgtggagtcg tagatcattc cgcgaggagt
     2041 actttcttgg accgtaatcg tagaagtaga cttcactcgc gcctccatct cttcgccgtt
     2101 cacgatctgc aaataaccca atctagcaat caacgcaaca aataacgtaa atataatgaa
     2161 aaaaagaaaa tttaagcgaa aaggaacatg ggaccggcga aacgttttgt tcttaggttc
     2221 ttgtgcacct tttcttattt tttctaacca agaagtattt aatatttttt tcataaaatc
     2281 tttcatcatt tcctatcctt ccgcctattt acattcatgc ttcattgtac ccaaaaactt
     2341 cttaataaaa aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa
     2401 tcaacttttt gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa
     2461 aaaataaact tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca
     2521 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
     2581 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
     2641 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
     2701 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
     2761 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
     2821 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
     2881 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
     2941 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
     3001 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
     3061 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
     3121 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
     3181 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
     3241 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
     3301 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
     3361 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
     3421 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
     3481 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     3541 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     3601 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     3661 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     3721 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     3781 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     3841 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     3901 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     3961 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     4021 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     4081 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     4141 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     4201 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     4261 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     4321 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     4381 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     4441 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     4501 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     4561 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     4621 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     4681 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     4741 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     4801 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     4861 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     4921 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     4981 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     5041 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     5101 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     5161 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     5221 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     5281 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     5341 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     5401 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     5461 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     5521 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     5581 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     5641 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     5701 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     5761 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     5821 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     5881 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     5941 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     6001 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     6061 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     6121 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     6181 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     6241 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     6301 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     6361 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     6421 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     6481 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     6541 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     6601 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     6661 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     6721 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     6781 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     6841 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     6901 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     6961 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     7021 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     7081 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     7141 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     7201 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     7261 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     7321 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     7381 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     7441 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     7501 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     7561 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     7621 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     7681 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     7741 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     7801 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     7861 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     7921 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     7981 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     8041 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     8101 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     8161 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     8221 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     8281 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     8341 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     8401 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     8461 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     8521 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     8581 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     8641 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     8701 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     8761 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     8821 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     8881 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     8941 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     9001 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     9061 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     9121 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     9181 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     9241 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     9301 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     9361 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     9421 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     9481 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     9541 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     9601 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     9661 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     9721 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     9781 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     9841 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     9901 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     9961 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
    10021 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
    10081 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
    10141 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
    10201 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
    10261 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
    10321 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
    10381 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
    10441 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
    10501 atatggatat tt
//
LOCUS       DACCCR010000086        10510 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCCR010000086 DACCCR010000000
VERSION     DACCCR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.093"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IVK93_002296"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IVK93_002296"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP6387565.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IVK93_002297"
     CDS             1085..2005
                     /locus_tag="IVK93_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6387566.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IVK93_002298"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IVK93_002298"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP6387567.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IVK93_002299"
     CDS             3311..4789
                     /locus_tag="IVK93_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6387568.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="IVK93_002300"
     CDS             5066..7126
                     /locus_tag="IVK93_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387569.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="IVK93_002301"
     CDS             7209..7778
                     /locus_tag="IVK93_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6387570.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="IVK93_002302"
     CDS             complement(7953..8759)
                     /locus_tag="IVK93_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387571.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="IVK93_002303"
     CDS             8897..10411
                     /locus_tag="IVK93_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP6387572.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3727 a         1752 c         2070 g         2961 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccaccta caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag
//
LOCUS       DACCCR010000087        10118 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCCR010000087 DACCCR010000000
VERSION     DACCCR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.094"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            107..613
                     /locus_tag="IVK93_002304"
     CDS             107..613
                     /locus_tag="IVK93_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP6387573.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            630..1529
                     /locus_tag="IVK93_002305"
     CDS             630..1529
                     /locus_tag="IVK93_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP6387574.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1616..2500
                     /locus_tag="IVK93_002306"
     CDS             1616..2500
                     /locus_tag="IVK93_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6387575.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2692..2763)
                     /locus_tag="IVK93_002307"
     tRNA            complement(2692..2763)
                     /locus_tag="IVK93_002307"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2728..2730),aa:Arg,seq:ccg)
     gene            2823..4157
                     /gene="rpoN"
                     /locus_tag="IVK93_002308"
     CDS             2823..4157
                     /gene="rpoN"
                     /locus_tag="IVK93_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP6387576.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4360..5397
                     /locus_tag="IVK93_002309"
     CDS             4360..5397
                     /locus_tag="IVK93_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP6387577.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5438..6439
                     /gene="gap"
                     /locus_tag="IVK93_002310"
     CDS             5438..6439
                     /gene="gap"
                     /locus_tag="IVK93_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6387578.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6549..7739
                     /locus_tag="IVK93_002311"
     CDS             6549..7739
                     /locus_tag="IVK93_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP6387579.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7843..8598
                     /locus_tag="IVK93_002312"
     CDS             7843..8598
                     /locus_tag="IVK93_002312"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP6387580.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8714..10012
                     /gene="eno"
                     /locus_tag="IVK93_002313"
     CDS             8714..10012
                     /gene="eno"
                     /locus_tag="IVK93_002313"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP6387581.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3546 a         1865 c         2098 g         2609 t
ORIGIN      
        1 tcttttttgt atataaaaat cccaagtaaa tacataaaaa ataaaaaaat tgaaactttt
       61 agcgcacctt gtacgtcata ttaagtgagg cagaaaggag tggagaatga tttttactgg
      121 atataagaaa aaacggcaga tcgaaaaata ccgtcacctg ctgaatggac tcatcgaaac
      181 tgattatcaa aaaatgttcc gtatcacgat gaattacgta catgataaag aagaggcctt
      241 agatgtgatg caagatagtt ttcataaagc cctgaactat tttgaaaaag aaaaagagat
      301 cgagcatttt agtgcatggt tttatcggat attgatccgc acggcattag atagttggcg
      361 cagacaaaaa aggacgcctg tggacttatt tgaaattcaa gatatccccc aacagttacc
      421 tttagaaact tccgttgcag agctccatat gattttagat aaaatagaca gtccggaaaa
      481 agagattctg atcctctact tttttgaagg atttcgtttg aaagaaattg cgactatctt
      541 ggatatgaat gaaaacacag ttaaaacgaa aatgtaccgc tcattgaatg ctttacgtca
      601 aatcctagaa tgaaggagag aagagtgcca tgtattccca aaaagaaaat ttaaaaaaac
      661 taaagaaaac ttataaagaa caagcaattc ccaaagggct taaagaggag atccgccagc
      721 gttttattat cgaagagcaa tcatttataa aagcaaagcg gagaaagaga cgtctccaag
      781 ggttcggagt atctttggct atcgtaatga ttacaacaag tagccttctt tttaatagtc
      841 aagttcgaag ctttgcagaa gatttaccca tcttaggatc agtcattgaa ttgatcttag
      901 gcgaacgctt tactgatcga tctgaaaaaa tcgatattca ggtaccgaag atcaatacac
      961 aaaatgagaa agaaaataaa acaattcatg gattaaatca aaaatacttt cgcgaaggac
     1021 aagcagattt tgaaaaagct gaaaaagaat atggaaattt tgaaacggat cattaccaag
     1081 ttttgggaga ttatcagaaa atcgtagacg ataaccggtt tctcgtgatt gaaaggcaga
     1141 tcacccaaac agcagcagat agccatgttg aaaaaagata cgacacaatc gacaagaaaa
     1201 acagtgtcca gctctcactt ccacttttat tcaaagatga cacctatcta tctgttttga
     1261 caaaagaagt caaacggcag atggccgaac aagtcaagga agatccatct aagtactatt
     1321 ggactgagca agatattcaa gaagggacga tagaaaaacc aacacttgtt acgcctacac
     1381 gaagttttta tttgaacaaa gagcatcaat tagttttgac tttttctcaa tacgaaattg
     1441 ctcctggtta tatgggtaca ccagaatttg tcattcctaa gtccgtgaca aaaacgattt
     1501 tggcatcgga agattatttg gatcattaga cgagaagtag ttgcgactag cagtttcaat
     1561 tttcaagtaa gtacaagtaa ataataagaa gagaaaaaga gaaagaagag atatagtgaa
     1621 aagaaattat ttagttatca gtattattgc tttatccgtg cttttgtttt tcgaaattac
     1681 ttcggtggcc tccttgatca ccgaagtaaa aggacaaaca cactcttcgg agcattcgtc
     1741 acaatctttc aaacaatcag aacaatcaac cattaaaagc caatcaactg aaacagctaa
     1801 cgtcaaagaa gccattcaag aatcagcgga aaaggtacaa agtcagtcat cacaatcaac
     1861 acaagacagt acaaaaacga gccgagatcc ggctgtcaaa agcgtcgcaa tcagctttga
     1921 tgatggacca ggagcgacca caacaccgca gcttctgcga atcttaaaag aaaaaaacgt
     1981 gcatgccact tttttcgtct taggagaaaa tacggcgcag catcctgaaa tcgtgaagca
     2041 gacagctgaa gcagggcacg agattggcaa tcacacatac gatcatcaga atctggccac
     2101 tctttctgcc cagtcgatga cggaggaagt gacgaaagca gacactgaga tcaaaaaagt
     2161 cactggcaaa acccccactt ttgttcgtcc gccctatggt tctgtcacga atgtaggagc
     2221 aacgatcatc cagcgaccga tcatcgaatg gtctgttgat tcagaagatt ggaagacaag
     2281 aaatcctgat ttgattttgc agaagatcca agcaacagta tacgatgggg caattatttt
     2341 attccatgat atctatcctg aaacgattcg tgccgttcct caagtgatcg attatctgaa
     2401 agagcaaggg tatcgcatca cgacagttgg ggatttatta gggcatccaa cggctgtcga
     2461 gaactattat ggcagaaacg atcatcggcc agttcagtaa gtgagacaag tatctttaat
     2521 aaataacaca ttttaataag tagttgaaca cataactacc ttacttgcaa aggcttaaca
     2581 atatctatac aggtacaaca aaaaactgtc caccaatttt ctttggcgga cagttttcac
     2641 ttaaaaataa agaagcaact attcgtaaat tatgctaaaa tagcagtaga acgcgtcctg
     2701 agggaatcga acccccgtct caagaaccgg aatcttacgt gatatccact acactaagga
     2761 cgcaagcact gggataattg taactaaaca gataaaaaat tacaagggga ttggacaaaa
     2821 aaatgaaatt tgagcaaaat ttttcacaaa aacagcagca gacacagaaa ctagccatga
     2881 cgcaacagtt gcaacagtcc atccaagttt tacaatataa cagtgaggag ctgttggcat
     2941 tcgttgagaa tcaagcaatg gaaaatccat tggtggaagt tgttgagccg gaatggcagc
     3001 cagattacat aaaagcttct tcatcttctt atgaaggaga agagacaaat tacctaaatc
     3061 agattcctga tacaaaaggg tctttatttg attcattgat cgaacaagtc catttgaatt
     3121 accgagatac ttttttgagg aaaatcgttc tttatcttgt agaatacatc gatttgaacg
     3181 gatttttgac gattagttta gaagaagccg gaaaagagat tggagctacg cctatccaaa
     3241 tgctcgatgc gttgactctg attcagcaat tagaaccagc tggagtaggt gcacgtagtt
     3301 tgcaagaatg tctcatgctt caaacagaac gagatgatta tgcgccggaa ttagcttata
     3361 tcgtgttgga agaatgtttt gaggaactgg tggagcgaaa atggaaagaa atcgcacaac
     3421 gtttttctgt tgatctacat gctgttcagc agatattcga ctatttacaa acgttgagtc
     3481 caagccctgg ccggattttc gaccgtagca gcgaattgta cattattcct gatgtccggg
     3541 tattagtgga cgacgataaa aatgtacaag ttatctcaaa ccgaaaaaat cagccaaata
     3601 tacgctttca agagagttac ttcaaacaaa tgtcacaaca agcagataaa gaaactgaaa
     3661 attatttaaa agaacggaaa caggaattcg aatggttgaa gaaaacgatc ctacaacgtg
     3721 gggatacaat tttgcgggtt gctcaagtca ttgtttcccg gcaaaaagct ttctttatcg
     3781 acaaggaacg tccaatcaag cctcttacgt taaaggaagt tgcaactgag atagatgtgc
     3841 atgaatccac ggtcagtcgt gcagtcaacg ggaaatattt ggaaacagac ttcggtgtat
     3901 ttgaattaaa aaaatttttc actacacgaa ttcctacgaa tagtacagaa cagacagaag
     3961 acctttcagc agatacagcg aagaaaaagc tgcaggaatt agtcgatcaa gaagacaaaa
     4021 acaagccgtt gtctgaccag aaattggttg aattgctgaa aaaagatgag atagcgatct
     4081 caagacgaac agtagcaaaa tatcgtgatt tattaggaat cccgagttca tcaaaaagaa
     4141 aacgttatga taactaatca gtcaaaagac tagaaaaagt atttcacttt ttctagtctt
     4201 tttagttgaa aaacgagagc aaaactgtta gacttacagt cgtgaggttg agatttccct
     4261 ttattgatgt gaaattcgaa tagttaattt ctttttttat cgtatatggg tcgttttaag
     4321 tctacattgg acgtaaagtg tccaactaag gagatcgcta tgctggatga aataaaaatg
     4381 attgaatctg ttgcaccaga tattatcgaa gtgttgcaag aacgctataa aattttacga
     4441 aatatctatt ggatgcagcc gattggacgt cgaagtttat cggaaagtat ggggatcaca
     4501 gaacgagtat tgcgaacaga aacagatctt ttgaaaaatc tgaatttgat cgacacctca
     4561 aaaagcggta tgacgctgac aactaagggc gaagaagttt atcaaagcct tgaaaacttc
     4621 atggatcagc ttttaggtac gcatcaaacc gaacaacagt tggcagaata ttttggtaac
     4681 caacgctgta tcgttgtttc aggaaacagc gaagagcaga ccaaagtggc tgatgcattc
     4741 ggcgaagcgc tgagcgaagc cctagaccga ctacttccag aaggcgagaa tatcatcgct
     4801 gtgatgggag gaactactat ggctgtggta gccgaacaat tgtctaatct ggaaaataag
     4861 aaacggcata acctgtttgt ccctgcaaga ggcgggattg gtgaagcgat aactgtacag
     4921 gcaaattctg tcagtgcaag aatggctgct aaagcaaacg gtaaccaccg tgcactttat
     4981 gttccggaac aattaagtct ggcaacttac aattctttac tcaatgagcc atctatccaa
     5041 gaagtcttaa acttgattag tgaagcgaat tgtgttattc atagtattgg gcgtgcatta
     5101 cacatggcag cacgtcgtaa gatgactgaa aaagaattag tcatgctgaa acaagcaaat
     5161 gctgttgcgg aatcttttgg ctactttttc aatgaaaaag gtgaagtcgt ttataaggtc
     5221 cctagaatcg gcatagaatt gcaagatttg gaaaaagtcc cgatcatcat ggcaatcgcg
     5281 ggcggaaaat caaaagctaa agcaattcga gcttatatga aaaatgcacc aaaacaaacg
     5341 tggctcatca ctgatgaagc tgctgcaaat gagattttaa aaggggtaac cctttaaaat
     5401 aaatattttt ttgattttca taaggaggaa atcttaaatg acagttaaag taggtattaa
     5461 tggttttgga cgtattggac gtctagcatt ccgtcgtatc caagatgtag atggaatcga
     5521 agtagtagca atcaacgact tgacagatgc taaaatgttg gcacacttgt taaaatacga
     5581 cacaactcaa ggacgtttca acggaacagt tgaagttcat gaaggttcat tcaacgtaaa
     5641 cggaaaagaa gttaaagtat tagctaaccg caacccagaa gaattaccat ggggcgaact
     5701 aggcgtagat atcgttctag aatgtactgg tttcttcact tctaaatcag cagctgaaaa
     5761 acatttaaca gctggtgcta aacgcgttgt tatctctgct cctggcggaa acgatgttcc
     5821 aacaatcgtt tataacacaa accacgaaac attaactggt aaagaaacag ttatctcagg
     5881 tgcttcatgt actactaact gtttagctcc tatggctaaa acattgaacg acaaatttgg
     5941 tgttgttgaa ggattaatga caactatcca cgcttacaca ggtgaccaaa tgactctaga
     6001 cggacctcat cctaaaggtg acttccgccg tgcacgcgct gctgctgcaa acatcgttcc
     6061 taactcaaca ggtgctgcta aagctatcgg tttagtaatt cctgaattga acggtaaatt
     6121 agacggagct gctcaacgtg ttcctgtacc aacaggttca ttaacagaat tagtaacagt
     6181 tcttgaaaaa gaagtaactg ttgacgaaat caatgcagca atgaaagaag cttcaaacga
     6241 atcttacgga tacaacacag acgaaatcgt ttcttcagat atcgttggta tgacttacgg
     6301 ttcattattt gatgctacac aaactaaagt aatgacagtt ggcgacaaac aattagttaa
     6361 aactgttgct tggtacgaca acgaaatgtc atacactgca caattagtac gtactttaga
     6421 atacttcgct aacttataag atagacaaac gtacaagtgt aaattataga catgtacatt
     6481 taaaagcggg gaagcaatcg cgcttcttcc gctttttttg ataataagtt tattcaggag
     6541 gaaataaaat ggctaagaaa acagtgaaag atatcgattt aaaagacaaa aaagttcttg
     6601 tccgtgttga cttcaacgtt cctttgaaag acggcgtgat cactgacgac actcgtatca
     6661 aagcagcatt gccaacaatc aactacgttc tagaacaagg cggaaaagca atccttttct
     6721 ctcaccttgg acgtgtaaaa acagaagaag ataaagaagg aaaatcttta gctccagttg
     6781 caaaacgtct aggcgaatta ttaggtaaag aagtaacatt cgtacctgaa actcgcggag
     6841 aacaattaga agaagcaatc cgcaacatga atgacggcga cgttgtcgta ttcgaaaata
     6901 cacgatttga agatattgac ggtaaaaaag aaagcaaaaa tgatacagaa ctaggtaaat
     6961 actgggcttc attaggcgat gtatttgtta acgatgcttt cggtacagct caccgtgcac
     7021 atgcttctaa cgtaggtatc gcttcaactg gtatcccaac agttgctgga ttcttgatgg
     7081 aaaaagaaat caagttcatc ggtgaagcag tagaagaacc aaaacgacca atgattgcaa
     7141 tccttggtgg cgcaaaagtt tctgataaaa tcggcgtaat cgaaaacttg attcctaaag
     7201 cagataaaat cttgatcggt ggcggaatga cttacacatt ctacgaagca aaaggaatca
     7261 agatcggtaa ctctctagta gaagctgata aagtggaact agcaaaagaa ttaatcgaaa
     7321 aagcaggcga caaacttgtc ttgccaatcg acaatgtctg tgcaccagaa ttttcaaatg
     7381 atgttgaaac acaagtaatc gaaggagaca ttcctgatgg tttgatggct ttagacatcg
     7441 gcccagcatc agtaaaatta tttgctgata cattacaagg tgcaaaaaca gttgtttgga
     7501 acggacctat gggtgtattt gaaatgagta actttgctaa aggtacaatc ggtgtttgtg
     7561 aagcaatcgc taacttagaa ggcgcaacaa caatcatcgg tggcggagac tcagctgcag
     7621 cagcagaaca attaggattt gctgacaaat tcacacacat ctcaacaggt ggtggcgcaa
     7681 gcttggaatt gcttgaaggt aaagaattac caggactagc agcaatcaac gataaataaa
     7741 cgaccaagtt tgctcaattc tgacgagtaa tcgatcaaaa ggtgaaggga gttttcatga
     7801 ctccctagcc atttttcaat aaattaagga gtgtgttcct caatgcgtaa acctattatc
     7861 gcaggtaact ggaaaatgaa caaaactgca tcagaagcaa aagaatttgc tgaagcagta
     7921 aaaaacaaaa tcccttcaaa tgacaaagtt gattcagtca tcggatctcc tgcattattc
     7981 ttacaagaat taatgacatc agctgaaggt acagaactaa aaatctcagc acaaaactgc
     8041 tactgggaaa actcaggtgc gttcactggt gaaacttcac cagcagctct tgctgatcta
     8101 ggtgttcatt atgtgatcat cggacattca gaacgtcgtg aatatttcca tgaaacagac
     8161 gaagacatca ataaaaaagc aaaagctatc tttgcaaatg gtatgacacc aatcctatgc
     8221 tgcggcgaat ctttagaaac ctatgaagca ggcaaaactg ctgaatggat cgaaggacaa
     8281 attacagctg gtttagctga tctttcagct gaacaagtgt caaacatggt tattgcttat
     8341 gaaccaatct gggcaatcgg tactggtaaa tcagctgacg cacaaatcgc tgacgaaatt
     8401 tgtggagttg tacgtcaaac agttgaaaaa ctttatggaa aagaagtatc agaagcagtt
     8461 cgtatccaat acggcggttc tgtaaaacca gaaaatattg ctgaatacat ggcaaaagaa
     8521 aatgttgacg gtgctttagt cggtggcgca agcttgcaag cagattcatt tttgtcattg
     8581 ttagacgctg taaaataatt gttgaaaatt tgcgaaaaat gcaagaaatg tttgcactaa
     8641 tgagcaaaat taactaaaat cgtatatgag ggaaatccct taggaaaaaa caaactcaaa
     8701 ggagagacaa aacatgtcaa ttattactga tgtttacgct cgcgagatct tagactcacg
     8761 tggtaaccca acaatcgaag tagaagtata cacagaaagc ggagcttttg gccgcggaat
     8821 ggttccttca ggtgcttcaa ctggtgaata cgaagcagtt gaattacgtg acggagacaa
     8881 agctcgttat ggcggtaaag gtgtaactaa agctgtcgac aacgtaaaca acatcattgc
     8941 tgaagcaatc attggctacg atgttcgtga ccaaatggct atcgacaaag ctatgatcgc
     9001 tttagacgga actcctaaca aaggtaaatt aggtgcgaac gctattttag gtgtttctat
     9061 cgctgttgca cgtgcagctg ctgactacct agaagtacct ttataccact acctaggcgg
     9121 attcaacaca aaagtattgc caacaccaat gatgaacatc atcaacggcg gatcacatgc
     9181 tgataactca atcgacttcc aagaattcat gatcatgcct gttggcgctc caacattcaa
     9241 agaagctcta cgtatgggtg ctgaagtatt ccacgcatta gcttcaatct tgaaagctcg
     9301 cggattagca acttctgttg gtgacgaagg tggatttgct ccaaaccttg gttcaaacga
     9361 agaaggtttt gaagtaatca tcgaagcaat cgaaaaagct ggctatgtac ctggtaaaga
     9421 cgttgttctt gctatggatg ctgcttcttc agaattctac gacaaagaaa aaggtgttta
     9481 cgtactagct gactcaggcg aaggcgaaaa aacaactgac gaaatgatca aattctacga
     9541 agaattagtt tctaaatatc caatcatctc aatcgaagat ggattagacg aaaacgactg
     9601 ggacggattc aagaaattaa ctgacgtatt aggcgacaaa gttcaattag ttggtgacga
     9661 cttgttcgta acaaacactc aaaaattatc tgaaggtatc gaaaaaggaa ttgctaactc
     9721 aatccttatc aaagtgaacc aaatcggtac attaacagaa actttcgaag ctatcgaaat
     9781 ggctaaagaa gctggctaca cagcagttgt atctcaccgt tctggtgaaa cagaagattc
     9841 aacaatctct gatatcgctg ttgcaacaaa tgctggccaa atcaaaactg gttctctatc
     9901 acgtactgac cgtattgcta aatacaacca attactacgt atcgaagacc aacttggtga
     9961 agttgcagaa tacaaaggtt tgaaatcttt ctacaactta aaaaacaaat aattcttttc
    10021 taatgaagat acatgatcga ctattgaaag atagtcgagt atgatcactt agttaagtaa
    10081 tcgaactctc tacttgctga aatatcagca ggtagaga
//
LOCUS       DACCCR010000088         9964 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCCR010000088 DACCCR010000000
VERSION     DACCCR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.095"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            8..81
                     /locus_tag="IVK93_002314"
     tRNA            8..81
                     /locus_tag="IVK93_002314"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IVK93_002315"
     tRNA            101..173
                     /locus_tag="IVK93_002315"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IVK93_002316"
     tRNA            201..274
                     /locus_tag="IVK93_002316"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IVK93_002317"
     tRNA            288..361
                     /locus_tag="IVK93_002317"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IVK93_002318"
     tRNA            385..474
                     /locus_tag="IVK93_002318"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IVK93_002319"
     tRNA            487..560
                     /locus_tag="IVK93_002319"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IVK93_002320"
     tRNA            579..654
                     /locus_tag="IVK93_002320"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IVK93_002321"
     tRNA            656..726
                     /locus_tag="IVK93_002321"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IVK93_002322"
     tRNA            740..813
                     /locus_tag="IVK93_002322"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IVK93_002323"
     tRNA            838..925
                     /locus_tag="IVK93_002323"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IVK93_002324"
     tRNA            947..1018
                     /locus_tag="IVK93_002324"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IVK93_002325"
     tRNA            1041..1130
                     /locus_tag="IVK93_002325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IVK93_002326"
     tRNA            1143..1216
                     /locus_tag="IVK93_002326"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IVK93_002327"
     tRNA            1235..1307
                     /locus_tag="IVK93_002327"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IVK93_002328"
     tRNA            1311..1383
                     /locus_tag="IVK93_002328"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IVK93_002329"
     tRNA            1388..1458
                     /locus_tag="IVK93_002329"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IVK93_002330"
     tRNA            1471..1544
                     /locus_tag="IVK93_002330"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IVK93_002331"
     tRNA            1556..1627
                     /locus_tag="IVK93_002331"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IVK93_002332"
     CDS             1893..3086
                     /locus_tag="IVK93_002332"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP6387582.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IVK93_002333"
     CDS             3216..4688
                     /locus_tag="IVK93_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6387583.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IVK93_002334"
     CDS             4771..5559
                     /locus_tag="IVK93_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP6387584.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IVK93_002335"
     CDS             complement(5626..6294)
                     /locus_tag="IVK93_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6387585.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IVK93_002336"
     CDS             6409..8121
                     /locus_tag="IVK93_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387586.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IVK93_002337"
     CDS             8124..9896
                     /locus_tag="IVK93_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387587.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2900 a         1781 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DACCCR010000089         9939 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCCR010000089 DACCCR010000000
VERSION     DACCCR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.096"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(33..1076)
                     /locus_tag="IVK93_002338"
     CDS             complement(33..1076)
                     /locus_tag="IVK93_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6387588.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="IVK93_002339"
     CDS             complement(1237..1920)
                     /locus_tag="IVK93_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HAP6387589.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="IVK93_002340"
     CDS             2115..3044
                     /locus_tag="IVK93_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP6387590.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3104..3895
                     /gene="phnC"
                     /locus_tag="IVK93_002341"
     CDS             3104..3895
                     /gene="phnC"
                     /locus_tag="IVK93_002341"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAP6387591.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3867..4646
                     /gene="phnE"
                     /locus_tag="IVK93_002342"
     CDS             3867..4646
                     /gene="phnE"
                     /locus_tag="IVK93_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP6387592.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4643..5425
                     /gene="phnE"
                     /locus_tag="IVK93_002343"
     CDS             4643..5425
                     /gene="phnE"
                     /locus_tag="IVK93_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP6387593.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5428..6231
                     /locus_tag="IVK93_002344"
     CDS             5428..6231
                     /locus_tag="IVK93_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP6387594.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6315..7862
                     /locus_tag="IVK93_002345"
     CDS             6315..7862
                     /locus_tag="IVK93_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP6387595.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8159..8356)
                     /locus_tag="IVK93_002346"
     CDS             complement(8159..8356)
                     /locus_tag="IVK93_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387596.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8432..8632)
                     /locus_tag="IVK93_002347"
     CDS             complement(8432..8632)
                     /locus_tag="IVK93_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6387597.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8752..>9939)
                     /locus_tag="IVK93_002348"
     CDS             complement(8752..>9939)
                     /locus_tag="IVK93_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6387598.1"
                     /translation="IYKGTLLYKPEECSHCLCVVPSRIIRWGTTTVRLLLNDASEYRT
                     YLELKKQRFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNI
                     STSSVYRVMKRFYRPLNPFKQALPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIV
                     ENRRLPYLECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHI
                     GRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDY
                     LTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTL
                     RTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTF
                     LTK"
BASE COUNT         2894 a         1976 c         2037 g         3032 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc ggcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gttagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt ttttgcttac acttaaaaaa atggagaagg ataatgatta tttttgacaa
     3121 tgtttcgaag gtctatccca atggcgttgt tggcttgaag gatattgact taacaattga
     3181 agatggagaa tttgtttcca tcatagggtt gagtggtgct ggaaagagca ctctgctgcg
     3241 cagtatcaat cgcttggcag aaaccaccaa aggagatatt cgcattaatg gaacctctat
     3301 tacaaaagcg aataaaaaag agctgcgaca gattcgccgc cagattggtc taatctcgca
     3361 aagcttcaat ctggtaaagc gtagtacggt tcagaaaaat gtgctatctg gacgtttggg
     3421 ctattactcc acttggaaaa gtattttagg tctttttacg aaggaagact accagcagac
     3481 aaaggaagca ctggaaaaag ttggtttgtc tgataaattg cagacacgca gtgatgagct
     3541 cagtggtggg caacaacagc gagtatccat tgcgcgagca ctcgtgcagc aagcccccat
     3601 tattttagcg gatgaacccg ttgcatcatt agatccaatc acgactcaaa aggttatgat
     3661 ggacctaaaa aatatcaatc aaacgatgga taaaacagtc attgtcaatc tgcattcagt
     3721 ccctctggct cgtgagtttt ctacgcgtgt gctcgcgtta aaagctgctc aggttgtctt
     3781 tgatggtcgt ccagacgact taaccgatga acgcttagag caaatttatg gcaatgcaat
     3841 ctttgaagaa aaggcaggcg aaatcgatga gcttgaaaga aaccgttcac tttaaatggt
     3901 ataaacacct gcttattctt ctgttcttaa ttgtttgttt tcaaggaagt gcctggatca
     3961 cgtcagctga tttttctcag gtttttcaaa actccaatca aatgactgtc tttttatcac
     4021 gcttcattca tccagacttt agctatttac ctaaattagt cgagcctatg gtgaaaacac
     4081 ttcaaatgtc tttgcttgga acggtcattg gcttaatttt agcgatccct gttagttttt
     4141 tagcgaccac tgtagtgaca gaaaaccgtc ttctttctac catctttcgt tttcttttag
     4201 gtttagtacg gaccatccct accctgcttt tagccgcgct ttttgttgct gtgtttggaa
     4261 ttggtgaagc gactggcgtg ttgactatcg ctgtctttac ttttggtatg gtgtcacaat
     4321 taatgtttca agcaattgaa accattgact ttggcccgat tgaagccgct gcggctgttg
     4381 gagcaaataa aatgcaaatc gctatttggt cgatcgctcc acaagttttg agtcagtttg
     4441 cgagctatgc tttttacgcg tttgaggtga atgttcgtgc ttctactgtc cttggatatg
     4501 tcggagctgg tggtattggc gttattctaa attcctcact tgcgcttttg aattatgaac
     4561 gagtttcaat cattattcta acgatcctag taactgtcgc gatcgttgat aaattaagtg
     4621 aacgtgtcag gaggtccctg ttatgatgct tcgtgaaatg aacagaagaa cccttttatc
     4681 attagttgct ttagtgattc tcgttatttt gtcggccgct actctggatt actctggatt
     4741 aagcaccttc tctccttcca tgggaatgga cgttttgaga gggttaggtc agcctgattg
     4801 gcagtttcta tatgatggca gcggcgagga tttgattagt ttgctattat taaccattgg
     4861 aatcgcctgt ttagggacaa tgatcgctac ggtattagcg attcccatta ccttgatcag
     4921 tgcggttaac ctctggcaag cacatccatg ggtaacaaaa attggcaagc ttatctgtaa
     4981 tgttttacga gcttttcctg aactagtttt tgcaattatt tttgtgaaag tcgtcggacc
     5041 tggccccttt gctggtgtta tggcgattgg cgtccatcag atcggaatgc ttggcaaact
     5101 ttttacagaa gaaatggaag cgatggatga gcgacttgtt gaagaaatgc atgcggtagg
     5161 agcaaatttt tggcaaacga ttttctttgt tcgcatcccc tatttgatgc cgatttattg
     5221 ttctttagca ttaaatcatt ttgaaatagc ggttcgtagt gcggctacct taggcttagt
     5281 tggcgctggc ggaatcggtg ctccattgat attcgcgatt caaactcggt cttggagcaa
     5341 agtgagtatt attttgattg gcgtagtcgt aaccgtgttt attttagatc aaataaccgg
     5401 aattataaga aagagattga gataagaatg aatatattgc atatatcaga tatacacttt
     5461 cgcagagagt atgaggcttg tgaagaaggc tataagggaa tgctggctac gatgcagaat
     5521 ccccttatcc ctctggagca atgcattcat catctcctgc agcaaacacc cttggacctt
     5581 gtgattatca gcggagattt aaccgaagat ggcgaaatag acgattatcg atacttgaag
     5641 acttggctgg aaaatgcgct aggagaaacg acgattcttg ttactctagg caatcacgat
     5701 atcaaagaac atttttgggt aggctggtgc gatgaagccg cctcttctgc cccgtacaac
     5761 cagatagtca aatatcctga gtttacagtg atttcctttg ataattcaag ctatggatac
     5821 gcagatggta tagtggacga tcagcaattt caatggctga aaaaagcctt agagcaagaa
     5881 aaagaccaac cgattttctt ggtcacccat catcatctac tgtctcacca aagcagtata
     5941 ccaaaatggc ctggtaccga acgtttcttg aagctaatcg caccttacga tattcgctgt
     6001 atcctaaacg gtcataccca tcacacattt actgatgaaa ttaatgggat cccctatttt
     6061 actgtatcga gtatgtcctt tgttggtgaa gatgaaggcg atggcttcgt acgctttgaa
     6121 gaacgtcatg ggtataacct ttatcatctc gatcaaggaa ggattgttcg ccagacaaca
     6181 gaaaacttta ttccaggcaa cattttaaaa acacttcaaa tgaaggatta aggcattata
     6241 aatcaaaaaa agagaccgaa cagctgaata cgcttcggtt tttctatttt gagtcagcta
     6301 aaggagcacg cagaatgaac attacgtttt tagagacaag tgatatgcat ggctatgttt
     6361 caccaacaga ctattccggc agaaccgatc tggccttagg aacggctaaa gtatcgtcga
     6421 aactgaagga attacgggca aaagcagatg gaccggtaat tacgatcgaa aatggtgatt
     6481 tcatccaagg gtctcctttg agttattatc ttgctaaaga atatggttca gccaaagagc
     6541 ttacagactt gattaaccaa atgagctatg atgttcaagt gttgggaaat catgagttta
     6601 actatggtgt ggattatctc gaggaagcga ttaagaatta tcaagcgcct atattaggag
     6661 ccaatatggt gaacaaaaat gaccaaccat actttggaaa cgcctataag attatcgaaa
     6721 aggtgggtgt aaaaattgct atcttgggat taacgacaca atacattcct cactgggaaa
     6781 agccagaaac cttagcagga attacgttta agagtatcgt cgaaacagca aaagaatatg
     6841 ttcctctatt aaggaaacaa gcagacttgg taatcgttag ttaccacggc ggatttgaaa
     6901 aagacttgtt aaccggtgag gacacagaag cgctaactgg agagaatgaa ggctacgagc
     6961 tattgcagca ggttgaaggc attgacgccc ttttcaccgg tcatcagcac cggaaaattg
     7021 ctgaaacggt tcatggagtt cccgttgttc aaccaggcta tcgtggcgag ttcatcggtg
     7081 aaattcgtct taccgtcgag aaaaaagagg ggcaagtggt tgttacagat caccaggttg
     7141 ctttacactc agttgaaaaa tcagagcctg atccagaaat tcttgcagcg atcgtaccgg
     7201 tagaagaaaa actagagaaa tggttggatc aacccttagg taaagtgaac ggaaatatgc
     7261 gtattaatga tccgatgcag gctcgtcttg aggaacatcc ctatgtagag tttattaatc
     7321 gtgtacaatt atttgccagc ggctcagaga tttctggaac tgcgttattc aataatgaag
     7381 gaaaaggctt tggtgagacg atcacgatgc gggacgtcat caccaactat atttatccga
     7441 atactctggc agtgattcaa ataaatggtg cagatttgag agcggcgttg gagcagaccg
     7501 caaattattt agctgtagaa aataaccaaa tcgtattcaa tccagccttt attcagccta
     7561 agccccagta ctacaattat gacatgtatg aagggattca gtacactatt gatatgaaac
     7621 agcctgttgg acaacggatt accgaacttc gttttaaagg tcaccccgtt cttccgcaac
     7681 aaatgctgga agtggtgaca aatcaatatc gggcaatcgg cggaggaaat tatgcgatgt
     7741 ttggtgcgga gaaaattgtc cgagaaatcc aaatcgacat gaccgaatta attgccgact
     7801 atttgaatat tcacccacgc atcgaagcaa aagtgaatca taattttcaa gtgattttgt
     7861 agtatataaa taaaaaattc aaaaaggtgt gagcagtttt ttgcccacac ctttgttatg
     7921 ccaaattgtt aggctgtctg atttccatcc agccattctt tagccagcga tctacctcat
     7981 caaaacaaaa aattttcctc tgatggttcc ttaaataaga ctattttgat cctttttcca
     8041 tgaataagat tttgcatcag acaatacttc atttctgaaa aagttgcggc accaatatag
     8101 cctaataccc ctttccgatc ttgattaaat acaaaaaata aatccgctgc tgcgatattt
     8161 aaaaaaaagt cgtcaagatt tctggatact ctttgtctag gatcggacat gagcgaggat
     8221 aatcaattaa ttcaatgcct tttttactga aatctttttc catatctcga tattcttctt
     8281 ggaaaacaat acttccagaa ataacagctt tcacaaaatt ggcctccatt tttttatttt
     8341 tcagacactc caccataccg gatacctctc aaagaaaaga catagcaaaa atcaatagca
     8401 gctatctctc cacggttttt tactggtaat tttatttttc ttttggaaat tgtatcagga
     8461 aggtcgttcc ttcatttttt cggctggtca cttcaattga accgtggtgc aggcgcacta
     8521 gttccttcac gattgataat ccaagacctg actcgcctat ctttgtattt ttacttgacg
     8581 gatcggcttt ataatagcga tcccaaatgt ttttttgttc ctcttcaatc atgccgatgc
     8641 cattgtcctc gacagtaata aatacaggct ctttgtcaaa tgatgttggt aaagagaaat
     8701 cttagaataa tttggacaga acagaaagaa gaaactctct ttctgttctg tttatttggt
     8761 taaaaatgtt ttgccatgac gaatcatgat ccgtagtttg aaattgtaaa agctgcgaaa
     8821 gccgtacgca ttgcgtttta atactttgat gtggttgttt aaacattcca atggtccatt
     8881 tgaatagctg tagtgaagcg cattttggat ctgtggcaga aactttttaa acgttcgtaa
     8941 agtggttgta taggtttcag gaagaccgct gacttctctg tttaaacagt cctcaaataa
     9001 tgactgatct cttttcttta ccgcataaag aatgtcttgg tataactgat agccttggcg
     9061 caggttggga caagcagata acagtcgatc gacaatctca ctttcagtca aataatcttt
     9121 aaaactggca cgccaaaccc ttttttcgta gtttaacttt gcttcttctt tttgtaggag
     9181 tttccaatag cgtttcaatt ttttccctaa ccctcgttga gagtgggaag ttcctttaag
     9241 ccgtgccgtg gtttctttca ctcgatgatt caagaaggtt ctgccaatat gttggacgat
     9301 gtggaaacgg tcgatgatca actgagcatt cgggaaaagc tttttcacca atgaaacata
     9361 aggcgcatac atatcaataa caatccattg aacggcttca cgagtcgcta atgaaaaccg
     9421 agaaaaatag cattctaaat aaggcaagcg tcgattttcg acaatatcca ataatgcatg
     9481 gctttgtccg tccatcatga taaaactcat tgcactggca acacttcgaa ccgatttgaa
     9541 ttcatcaaag cataagacct ttggcaaagc ctgtttgaaa ggattcaatg ggcggtagaa
     9601 tcgcttcatg acacgataga ccgaagaagt ggaaatattt ttctgacgag cgatttccgt
     9661 catggaagta ttctctttta agcgagcaat gacagaccaa cgaacttttt gactaatgaa
     9721 acagtgtttt tcagcaacag aggtatccgc tacgaatgtt ctttggcaag acttacattt
     9781 aaatcgttgt ttcttcaact ccagataagt gcgatactca gacgcatcat tcagtaacaa
     9841 ccgaacggtt gtcgtccccc aacgaatgat cctagagggg acgacacaaa gacaatggga
     9901 acactcttct ggcttgtaca gaagagttcc tttgtaaat
//
LOCUS       DACCCR010000090         9810 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCCR010000090 DACCCR010000000
VERSION     DACCCR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.097"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(34..2154)
                     /locus_tag="IVK93_002349"
     CDS             complement(34..2154)
                     /locus_tag="IVK93_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6387599.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="IVK93_002350"
     CDS             2384..3562
                     /locus_tag="IVK93_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP6387600.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="IVK93_002351"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="IVK93_002351"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP6387601.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="IVK93_002352"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="IVK93_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP6387602.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="IVK93_002353"
     CDS             complement(4915..6168)
                     /locus_tag="IVK93_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6387603.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="IVK93_002354"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="IVK93_002354"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP6387604.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="IVK93_002355"
     CDS             7746..9092
                     /locus_tag="IVK93_002355"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP6387605.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="IVK93_002356"
     CDS             complement(9400..9786)
                     /locus_tag="IVK93_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6387606.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2891 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta
//
LOCUS       DACCCR010000091         9555 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCCR010000091 DACCCR010000000
VERSION     DACCCR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.098"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..344
                     /locus_tag="IVK93_002357"
     CDS             <1..344
                     /locus_tag="IVK93_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387607.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            425..1045
                     /locus_tag="IVK93_002358"
     CDS             425..1045
                     /locus_tag="IVK93_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387608.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            1746..2030
                     /locus_tag="IVK93_002359"
     CDS             1746..2030
                     /locus_tag="IVK93_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6387609.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2033..2950
                     /locus_tag="IVK93_002360"
     CDS             2033..2950
                     /locus_tag="IVK93_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6387610.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            2943..3725
                     /locus_tag="IVK93_002361"
     CDS             2943..3725
                     /locus_tag="IVK93_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6387611.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            3726..4406
                     /locus_tag="IVK93_002362"
     CDS             3726..4406
                     /locus_tag="IVK93_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6387612.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4399..5295
                     /locus_tag="IVK93_002363"
     CDS             4399..5295
                     /locus_tag="IVK93_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6387613.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5490..7139
                     /locus_tag="IVK93_002364"
     CDS             5490..7139
                     /locus_tag="IVK93_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6387614.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            7733..8668
                     /locus_tag="IVK93_002365"
     CDS             7733..8668
                     /locus_tag="IVK93_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387615.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            8661..9431
                     /locus_tag="IVK93_002366"
     CDS             8661..9431
                     /locus_tag="IVK93_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387616.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         3056 a         1584 c         2040 g         2875 t
ORIGIN      
        1 caaatataaa aactagtacg aaattaattt gtaaattatt taagcttgat aaaataagca
       61 atatcgatga tgtaaagtcc aagatattaa caagaaaagt aagcgaacca gtaaagctaa
      121 cggcacaaga taaaaaaaat ggaattgttc tttatggtga tgatgcaaag tataactata
      181 attgggatac ctcctcagaa gctgaggtat taagtattcc tactaccgat acttcagggg
      241 aaaaagtcac ctttaaaata aattttataa cggcaatttc taagttggag tatgggaagc
      301 aaaaaaagag agataacaat gatcaatatc tacctaaaaa ataacgaatt aatgatgcat
      361 atgttaaagt tgtttttttg aattaaacaa ctcaatttat tcagttatat gttcatcgca
      421 gcaagtgaac attaatcgag ttataaaatt aataggagct aaaaatataa ataaagttcg
      481 atcagaattt atttttccag aagatttttt taactggttt ttttatcaat tcagtgaatg
      541 caagcgactc ttaggtgaga attattactt aaaaggaatc ggcggattta tggggaatgt
      601 ggctgatgaa caaaatatta taaaaggaat atctgaacaa acatcagagc ttacagtaac
      661 taaagcattt ataagtaatg gtgaaagttt gaaaaatgta actgcgcgaa ttagaaaaga
      721 aaatgaagta gacattgttt ttggaattga ccatgaatgt aattcagtga tatatgtaac
      781 acaatcagca aatttaattc tttttgagaa tggttgcaag gacgatttta tggtggtcta
      841 tttgtacgat atactaattc caaaacttcg ttccttatat atgtcttctg aaagtatttt
      901 tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt attgaagtaa ttgaatcgat
      961 tatgtgtcat aatcatatta cgctagaaga attagaaatt ttgatagggc aaaaaaataa
     1021 taacacgaaa atacaagttt cttagaaatc attttttatt gagatatcta gataaatgat
     1081 ttattcttca atcaaccaac tcgccaagct gaggtaatgt tttgaggtaa tgataacata
     1141 caacttttta taccctagta aaactctata aacaaagact tattaatttc gcatgaaata
     1201 cattcctgta aaatgcaata aaatagagtt ttgtttaacc aggtcttaag aaagcccgta
     1261 aagcttcaca gttctcaaaa cgttaattgc gattatcgtt cgagttcacg agttacgaga
     1321 atcgtactgg gcgaagcaat gctgagcgca gttaccttaa tcctaacttt tctggcactt
     1381 tccacaattt ggaaagtgcc ttttttgtgt ttttagctat gttaataggt aggattcttg
     1441 gtctataata tcattcgatc tataaacaaa cgggaaaaag ataaagtatc atcaaacaat
     1501 attatttatc cgcttctgag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa
     1561 tttacaaaaa ttgcttctca tgtttgataa atattgtgac ttgtgaccga ggagttggca
     1621 ggagaacacc gtttattcgg aagaagggat tgtgaaaagt cttttgtcat aacctcttat
     1681 ttggtagcta attgtaaatc ccaaacggca tgtattattt ttcataagtc cgtttttaag
     1741 gattaatgtt ttcttttgct ttttatatgg tctggcttca gcgagaagtt ccaacgcata
     1801 tacaaggacg tctgatgtca acgataacaa cgattaccat ggtggccagt ccacttggat
     1861 tagcactgtt tggtgcgctc tttgattggc agacaactca accacgtctg cgagacagtc
     1921 tgatttttct tgaaattcaa ttggaaaatt tacactttct ttatacattt ctggctgctt
     1981 tctttatacc cgtcaactat acttcactta tcaataagaa agaaggataa gtatggattt
     2041 tggaaatgaa acggtcttga aagcaactgg tataaccaaa aaatacggtg ctgccaaagc
     2101 attagataaa gtatcgatcg aaatcaaacg tggaatgatc tatggtttga ttggggagaa
     2161 tggagcgggt aagtctacct ttatgagaac tattatgggt ttgatctcga ttgatgaagg
     2221 ttcgatcgaa ttatttggaa cgacggattt gcaggcagca agacgacgta tgggtcaatc
     2281 gattgaaacc cctgctcttt atccagaatt aactgcaaga gataacctac gtatccaagc
     2341 agcgaacggc ggtgtgtcgg atagagaaat cgaagacctg ctgaaaatga tgcgattgga
     2401 aaatacaggg aaaaagaaag caaagaattt ctctttaggt atgcgccagc gtttagctat
     2461 tgcaaatgca ctgatcacga atccagaatt tttgattttg gatgaaccga cgaatgggat
     2521 ggatccagca gggatggcag aaatgcgaga aatcatccaa cgtttggtaa aagaacgggg
     2581 catcaccgtc ttgctttcta gccacttgct agatgaattg tcacagatcg ccactcacta
     2641 cggtattttg catgaaggac acttgatcaa agaattatcg aaagaagaat tggcacaaga
     2701 atctcgtcaa tttataaaaa ttgacacttc ggcgacggaa caagcagtaa cggttctcga
     2761 ctctcttggt tatagggatt attttgtcca atctagccgt gttatccagc tttttgaagg
     2821 aattgaccaa gtagcagcca tcaatcaagc attggtagag gcaaaagttc ctgtggatgg
     2881 tatccaccta gttgggcaaa aactggaaga ttatttctta caattaactg gaggcaatcc
     2941 acatgtttaa tctattaact gctgaaaaaa taaaagtatg gaagagccgt aaaatgtggg
     3001 tctcactagg actgatggtc gtacttccgc ttttttattc ttggaacgaa tggtatatgc
     3061 atacaaaata cggcaatgaa ttaaaccaag caacggatac agtcatcaac ggtgcaacag
     3121 ggattttaat ggtggaaaaa atggctgcat ggattcttct ggctttcgca gcatttgcgt
     3181 gcttttatat tggggaagaa tttcaaaacg gtacgattcg caatacgtta tctttaggac
     3241 ggaatcgaat gacttattat gtgtcgaaac ttctcgttac gcttttgatc acaacacttg
     3301 gcgtggtttt gattacgggg ctagccatga ttgcttacac acttgctttc ggatttggtg
     3361 aagttgaggg aatcaaaaac tatggaaatt atgttctaaa agtatttcct gtattgcttc
     3421 tgctgatatt agcgacatta tctgttccag tggctttgac attcatcact agaagtacca
     3481 gtgtcagttt gcttctttct tttctttata tcatgggaac ggccttcgtt cccggtgttt
     3541 tcgcaaaaat caaaggcttg gaatttctaa cggagtggtt tacggaaacg tggctcatgt
     3601 atacggactt tgcgcaacaa gcaacttatt cgcaagcacc gaaaatgatt ctggtcagtc
     3661 tagtaacaat cgtggtatca tcagctctag gaatgtttat tttccaaaaa tctgacatta
     3721 agtaggtgtt cctgctggaa actattttga ttatcgaaga cgatgaagct gttcattctt
     3781 tactggagga ggttcttgaa cagagataca aacttcttga tgcttattca ggtacggaag
     3841 gaaaattatt gttaagcaca tatcctgtcg atttgatcct tctagatctc atgcttccag
     3901 ggctttcagg tgaagcattg ttagcagaaa tccgacagac atcgaatgtt ccgatcatcg
     3961 ttctttcagc aaaaagcgat caaagagata aagtttcctt gttggcagca ggagcggatg
     4021 actatgtgac gaaaccattt gatatcgaag aactgttgtt acgaatcggc atccaacttc
     4081 gtcatcagac ttctgtccaa gtaaaagatc tgtcgcaacg catctattac aaggaaatct
     4141 tactggacaa agagtcgagg gatgtaaatg tgaatggtgt ggctgtccat ttgacaggaa
     4201 gagaattcga tctgctgaag ctttttttag aacatccgaa aaaagtattt tcgcgagcaa
     4261 atctttatga aacagtttgg caagaaccat attttgatag tgagaaaaca gtgaatatgc
     4321 atatttccaa tcttcgaggg aaactagcaa aggcacatgc tgtgtatatt catacggtgt
     4381 ggggagtggg gtttagattt gattaagtgg ctgttgcttc tcgttgttat ctttatggga
     4441 agttttattt tcttcctgca aagggaaatc aagagattaa ctaagaaaat cgccactctt
     4501 tctgaacgac aagaatatgg tggtcgtctt tcagtcgaat ttcgagaaaa gaatctaatg
     4561 gacctagtag atgcactgaa tacatgggtg gatgaaaatg aacaaaaagg acaagcattt
     4621 caagaaatgg aagaaaacat cagactatcg atcgtcggcc tttctcatga tctcagaact
     4681 cctttgactg ctattaacgg ttatgtacaa ttactggaac aggtagagga tacggagaaa
     4741 cgggagaagt atttaacgat catcaaacaa tctgttgcaa gattactcag tatgacggat
     4801 cagttttatg atcttgctcg aatagagacg aagcaaaaag aaatggatct ccatacgctt
     4861 tctttttcta aagaggtgga agaagtattt ttctcttttt atgaacaatt ttctgagaaa
     4921 aatatcgagg tagcgttcgc tgaacactcg ataaatacaa ctgtattagc agatccgctc
     4981 atgttgctgc gagtgctgca aaatatcata cagaatttat tgcgttatgc agatggacaa
     5041 gcaaaggtga cattttcaaa agaggattca ttcattcgat taacagtggc caatcctatc
     5101 aaaccaggag cgcaaacctc tgttgaaaaa gtcttccagc gtttttatac cgaaaatacc
     5161 agccgcacaa atacggaatc tagcggaatc gggctttacc tgtcaaaaaa attggtggaa
     5221 ggaatgggag gagagatgac cgctagatta gacgggggca tcttctccat ctccgtcaag
     5281 ttgcggaggg tttaattggc tctaatttac ctgaagtgaa tgacaaaaaa ttgtatctcg
     5341 ttttaaagac atcttctttt ttggaaggtg tttttatttt ggtgtgattg atacgaatta
     5401 ggtaacgaag acagttattt ttgtaccccc ttttcaaata gcgcaacagt gctatactaa
     5461 aactaacaaa tcaaggaacg gagagggata tgggccaaag agaatttatc gttttaatta
     5521 ttattgcagt tgttatttta ttagttgttt tagacatttt ttcacgtttg aaagtaaagg
     5581 aaacagttcg tagtaattgg ggaaaaattc cttatcaacc gagatttgat aaagaagaaa
     5641 gtttgaagga tgcgtggctc acggagaaaa aattccgttc atgggatagt gaaatcgacg
     5701 atcttacttg gtatgacttg gatatgttcg aagtatttga agggatcaac agtacctatt
     5761 ctagtgtagg ttcagaagcg ttgtatcagc gtttacgtag ctttgatttt ggagaagatt
     5821 atcagttaga aaaactaatt gctttttatc aggaaaatcc tcaactgaga gaaagaatcc
     5881 aatatcaatt cgctcgttta ggaaaaaaag atcataattt tgcaaaacaa tatctagcgg
     5941 atggaaaaag taaaaagatt ggacgcgtag gtttatttat ttttcttggc ttgcttccgc
     6001 tggtgggtgt aggtttgctt cttttagggc aagtactagg aatctatcta gcaatcggtg
     6061 gcttagtatt caatacggtc tattatctag tgaagaaaca aacactagaa acagagctga
     6121 atagcatgag ttatttggtt tcaacgattt cagccgccaa tcagttagca aaaatgacga
     6181 caccacttca aaaagagctg aaacagcaaa ctaaaccatt aagagacatc cctaaattag
     6241 gtttttcatt tcgaatgaag agcgggagtg aagcagaatt gatttttgaa tatgtcaaca
     6301 tcatgttcat gctgccattt atctcctatc atttcgttct cactaaatta gaaaagcatt
     6361 caactgaagc catcaagctg tgggaattgc tgggagaatt ggaagttgca gcagccgttt
     6421 tgaattatcg cacctatatg ccgatcactt gccgaccaga gtttactgag ggcggtgtga
     6481 cagcgaaaga tatctatcat ccattgttaa aagaagcggt agttaatcca gtcaactggc
     6541 agaagaatac attagtcacg ggttccaatg catcaggaaa atctacgtat gtcaaaagta
     6601 tagccatcag ctgtattcta gcacagacga tccaaacagc gattgcagaa cagtttaccc
     6661 tgcaacctgg acatgtgttg acttccatgg caatcgaaga tgatttattt gaaggcgaca
     6721 gttattttgt ttcagaaatc aaatcgatca aacgtttgct ggaccaagtg gcttccaaag
     6781 aacgctgcta ctgcttcgta gatgagattt taaaaggaac aaatacgatc gaacggattg
     6841 cttcttccgc ttcagtcgtt cggtggcttg cggagtatcc atctttagca tttgtcgcca
     6901 cacatgatat cgaattgaca gagattttaa agaattattg tgagaatgtc cactttgaag
     6961 aacaagtaac agaaggaaaa ggaatcagtt ttgatttcaa attaaaagaa ggaccttcaa
     7021 ggacccgaaa tgcgattgcc ttgctgaaag ttttgaatta tcctaaatcc atagttgaac
     7081 aggcccaaaa agaaagtttg ttgttcgatg aacagcgtca gtggtatccg tttgattaac
     7141 tttatcaaac gaagcatgat gcgtgggtcc aaaaagacga ttatcaaaag aagtacattg
     7201 atgccaaaaa agtggcagaa ttcaatggga acgagtctgc tgtccagatg atcttggaag
     7261 atttagcaaa agacgataca aaataaacca cgctgtcttc cttctagcag cttttatcga
     7321 aaaaacttag catcagcgac gagacgcacg ttccatggca aagttctccg taaaacaaac
     7381 agcgaaaaca gaaattgaaa ataactattt ccattttatt tatttcggtt aaaataaaag
     7441 aaaaaagctt tcgataaaag gacggaggaa agcttaacat gaataaaaaa gacaagaacc
     7501 tcagttataa ggatcttcta tatgtcatcg tagtagttgc tgtcggtact tttatgattt
     7561 taaaagttcc agagttgttt agatagttat tctatcgaag atgatcatga actagaaaac
     7621 acatctcctc tttgttagca ggtgtgtttt tgtgtggaag ccaagttgtt ttttcatctg
     7681 ctgtattcca tgatacaatt aagaatcatg tctataagga gataggaaat aaatggcaaa
     7741 tgcgcttgaa ataaataatt tgaaaaagat ctatggaagt ggggtcgaag cattgcgggg
     7801 aatcgatctt tcggtgaaag aaggagattt ctatgcgcta ttagggccaa atggagctgg
     7861 gaagtctaca acgatcggga ttattacttc tttagtcaat aaaacatctg gaaaagtgaa
     7921 tgtcttcggt tatgatctgg acaaggatct agttcgagca aagcaacaga ttggattggt
     7981 tccacaagag ttcaatttta acccttttga aacggtccag caaatcgtcg tgaatcaagc
     8041 aggctactac ggtgtctctc gcaaagaagc gatgaaacgc agcgaaaaat atttgaagca
     8101 gtccaatctt tgggaaaaga gaaatgtccg tgcacgtatg ctttccgggg gaatgaagcg
     8161 aagactgatg attgctaggg cattgatgca tgaaccacgt ttgctgatct tggatgaacc
     8221 aacagcagga gttgacatcg agttgagacg cgagatgtgg gagtttctac gagaattgaa
     8281 cgaaaatggt acaacgatca ttttgacgac gcattatcta gaagaagctg aaatgctttg
     8341 tcgcaacatc ggaatcatcc aatcgggtga actgatcgag aatacaagta tgaaagagct
     8401 tctgtcaaaa ctgcaatttg aaacatttat ctttgattta gcgccatatg ataccaaacc
     8461 tgtgattgaa ggatatgctt gtacatttga agatgagcag acaatcgctg tcgaagttga
     8521 acgaaatcaa ggggtgaacg gaatcttcga tcagcttaca agacaaggaa tcaaagttct
     8581 ttctatgagg aataagtcga atcgtttaga agaactgttc ttgaaaatca cagaagaaaa
     8641 taatcatacg gaggagaaaa atgtttaatc tttattttac tgcattaaaa agtttagcta
     8701 tcaaggaaac gaaccgctat ttgcgtatat gggtccaaac actggttccg ccagtgatca
     8761 cgacatcctt gtattttgtt attttcggaa aaatgatcgg tgggcggatc ggtgacatgg
     8821 gtggtttttc atatatggaa ttcatcgttc ctggtttgat tatgatgtct gctatcacta
     8881 gttcgtatgc caatgtttct tcgtccttct tctctcaaaa atttcagaaa aatatcgaag
     8941 aaatcttggt agcacctgtt cctacgcatg tgattatttg gggctttgtg attggtggtg
     9001 tgggtcgcag tattctcgtc ggctctctcg taacgatcat ttccttgttc tttgtaccac
     9061 ttcatgtgta ttcatggttc atggtcatca ttacattatt gatgacggca atcttgtttt
     9121 cattagcagg actgctcaac gggatttttg ctcagtctta tgatgatgta tcgatcgtgc
     9181 ctacatttgt cttgcagccg ttaacttatc taggtggcgt tttctatgcc atctctatgt
     9241 taccgccctt ttggcaagct gtttcaaaag tcaatccgat cgtttatatg atctccggtt
     9301 tccgttacgg attccttgga acgatcgatg tgccagtagc aacatcgatg atcgtacttg
     9361 tgttattcat cgttgttttg tacgccgtta cttggtattt gatcaataaa ggaagaggat
     9421 tgagaagtta gaaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
     9481 cgggaaaata gttcctcata ttttgttgtt ttttgagcga ataccgaggg tcagctgatt
     9541 gaacaccgtt tagtg
//
LOCUS       DACCCR010000092         9468 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCCR010000092 DACCCR010000000
VERSION     DACCCR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.099"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1079
                     /locus_tag="IVK93_002367"
     CDS             <1..1079
                     /locus_tag="IVK93_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP6387617.1"
                     /translation="QGYDLIEDQRHVLSSILDIAKEEKVEAIVIAGDLYDRSVPAVDA
                     VELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSNYFLRTQLAEAFEPVE
                     FKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQELFDSEKKQVLISHFFV
                     MGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALRQDNARYSGSPLKFSL
                     SEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLDPAFYQSVNRENYIYI
                     YLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLEMKDPQKLAEAFFSEM
                     TEEQLTANQEKWLGETLQEIMEME"
     gene            1086..4214
                     /locus_tag="IVK93_002368"
     CDS             1086..4214
                     /locus_tag="IVK93_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP6387618.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            4233..7427
                     /locus_tag="IVK93_002369"
     CDS             4233..7427
                     /locus_tag="IVK93_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6387619.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(7573..8280)
                     /locus_tag="IVK93_002370"
     CDS             complement(7573..8280)
                     /locus_tag="IVK93_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6387620.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            9045..9443
                     /gene="spxA"
                     /locus_tag="IVK93_002371"
     CDS             9045..9443
                     /gene="spxA"
                     /locus_tag="IVK93_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP6387621.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         3420 a         1615 c         1926 g         2507 t
ORIGIN      
        1 tacaaggtta tgacttgatt gaagaccagc gtcatgtact atcttctata ctagatatcg
       61 cgaaagaaga aaaagtagag gcaatcgtga ttgccggaga cttatatgat aggagtgtcc
      121 cagctgttga tgccgtagaa ttatacaatg aattgatggt cgattggaat ttaaaggaaa
      181 agatcccgat ttttgctata tcaggaaatc acgatagcag tacgagactt tcagcgggta
      241 cgccatggtt cagttattcg aactattttt tgcgaacaca gctagcagaa gcatttgaac
      301 cagtcgagtt taaaaacgta caatttttct ttcttcctta ctttgaacca attgcagcga
      361 gattatattt tgaagatgac aatatccgaa cgatcaagca agcggtagcc aaggtgatag
      421 ataaaatgca ggagctattt gattcagaaa aaaaacaagt attgattagc catttttttg
      481 taatgggaag tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg ttggattcga
      541 tccctggtga actacttaca ccgtttgatt atgtagcact aggacatttg cataatcaag
      601 ctgctttgag acaggataat gctcgctata gcggttcccc attgaaattc tctttatctg
      661 agatcaacca aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg gagttgaatt
      721 ttagggagat cgaacccctg catgatattg aagaaataac agcgagtttc aaagaattgc
      781 tggatcctgc cttttatcag tccgtcaatc gggagaatta catctatatc tatctcactg
      841 atagaacgat catcccgaat atgatgaacc agcttcggca aatatatcct cgtattttgg
      901 ggattgaacg ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa
      961 tgaaagatcc tcaaaaatta gcagaagcat tttttagtga aatgacggaa gagcagctaa
     1021 ctgcaaacca agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa atggaatgat
     1081 caagaatgaa accgaaaaaa ttacaactta aaaattttgg tccttttatc aatgagaccg
     1141 tagatttttc acgtttgaca gaagcaccgc tttttttgat cagcgggaag acaggagctg
     1201 gcaaaacgac gatttttgat gggatcacct tcgcactttt tggtgaaact tccggaagac
     1261 ttcgctctgg aaaagagatg cgttctttgt ttgctacacc agacgaagaa acaagcgtga
     1321 ctttttcttt tgaacatcag cagatgagct atgaaatcga acgaaaacca gaacaagttt
     1381 tagcaaaacg aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt
     1441 ttgatgggaa aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca gatcagttga
     1501 tcagagaact gatgaatctc gatgcgaaac agttttctca aatcgtttta ctaccccaag
     1561 gagaattccg taattttttg gtttcatcaa gcagtgataa agagactgtt ttacgtcatt
     1621 tattcggtac tcaatttttc caacagttca atgagcgatt aaaagaaaaa gcaaaacaac
     1681 agcaacaaaa attggatcat ttagagcaag agctgcatct tctgcaaaaa aggtttgttt
     1741 tagtcaaaga agaggaacca gcagcagggt ttgaagccgt tttagcccaa tggacaaacc
     1801 atcaggcaat actcgaaaaa caaatcacgg aagaaaaaga agctctaacg gaacttcagg
     1861 aaaaacaaaa gcaactagaa aattcctact atcgttttga aagcttgaaa aaaagttatg
     1921 aagaaaaaac gatattaata aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag
     1981 aggaaaagaa acgatggatt gggtattatg aatttacgca acgattagtc gaaccaatcc
     2041 agcgcctaca agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa
     2101 caaagaaact agcgatgact acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc
     2161 gcgtacgccg acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac aaacagatga
     2221 tggtgccaat tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt
     2281 cattggataa agaagaattg aatgccgaga agctgtatca aaatctcgct atctatcagg
     2341 aaagaactcg aattcttcaa gaaaatttac tggatcagga aaaactgcaa gaagatcgtc
     2401 ttgcttttgg acagttaaaa tatctagatg aaaagcagca agaaatagaa aaacaaaaag
     2461 caaaagacac tgggtcgctc gcacagataa gagaagaact caaaaaagca actgaagaaa
     2521 taacaaaaaa tagtcagcta ttgaaagaac agacagaaca agcagaacag ctgaaaagcc
     2581 aatgggcaaa gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg gagccatgtc
     2641 ttgtttgtgg atcaacggaa caccccatac aacaattaaa acacgaacag ccaaataagg
     2701 aagatatctt aaaagtagaa caacaactgg cagaagctga gtctgctgta gaaagaactc
     2761 agcaaatcat tgccaaaagt gaagcacatg aaaataatct tcaacaacaa ttatcagatt
     2821 tagaacaaaa aatcatggca acgaatatac aattggctga acaagaagaa cacttgcaac
     2881 aggctttgaa aaaggcattt cctgagataa cagaacaaaa aacaaaaaca actcttgtag
     2941 taatcgaaca atctttagat agacgaaaaa gcaagatggc caatgcaaga atagagctgg
     3001 aagaaataag acaacaagca gaaaagctcg aacaacaatt agctgtctac aaacagcagc
     3061 agacagaaaa aaataatcag ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac
     3121 aattgcaaga tacggatagt aactgtttag aagaggagat acgacgactt gaaaaacagt
     3181 tagaagaaga ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag
     3241 atcttcatac gcttcggaca cagcttgatc ttcaacatga acaaatagaa aaaatagatg
     3301 aaaaaataga aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag tcgtattttt
     3361 caacaattga agaaatctgt gcatttgcac aacagcaagc acaatataaa gaaaaccaac
     3421 gtacagttac ttcttatcat gaagagcaat tagtgacagc tgaccggctg cgtcaattag
     3481 aaaatatcca gctaactgag acattcccag atacagaaga gatcagccaa cagttagaag
     3541 aaataagacg tatgatcgaa ataaagcaag aaaagatata cggtttacaa gaacaaaaaa
     3601 ttagtaatca aaaaatctac gatgaatgtc tttcgattta tcacacaagc cagacgaaat
     3661 tagatgagct tacgcaatta cagcagcttt cgcaaacatt caacggagaa aatcctaaga
     3721 agactagttt ggaacggtat gtgcttcagg tttatcttca agaagtgtta caagtagcaa
     3781 atgatcatct ccaacgttta acgaaaaatc gttatcaatt tgagctagcg gataccattg
     3841 gcagttatcg agggaaaacc ggattagaaa tcaatatcta tgatgatcag gcaggtatgt
     3901 ctcgaagtgc gcataccctg tctggcggtg agagttttat tgcagcactt tctttagcat
     3961 tagctcttgc cgatgtgatc cagacacaag ctggtggtgt agcaatcgat gcattgttta
     4021 ttgatgaagg atttgggtca ttagatgaag aagctttgga aatggcaatg gaagctttag
     4081 aaacgatcga aagtgaagga agaatgatcg gcatcattag tcatgtaaga gaattgaaag
     4141 aacgagtatt acaacaaata agaattgata cagaaggtag cggacaaagc aaaatacgct
     4201 atcaacttgg ataaaaaggg agattggtaa ctatgaaatg gagtattcca gaaaaagtca
     4261 ttgcacgtgg acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag
     4321 aagaaaacgt gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt gatttggatg
     4381 gtacagcaaa agagatagat tactgccaat gtccctattg ggaggaacat aaatattgta
     4441 agcatacagt tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa
     4501 aagaccagcc aatgctagaa ccggtaaaag caacaagtga gggagaaatc attgcaaaag
     4561 gtttttctcg tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg attattgaat
     4621 accaagtaga aacgattgag actaatcagt atcacccaga gctttctatt ttggcacttt
     4681 acttgcgtat cggttcattg gcatcaccga aaaaaacata tatcgtaaaa aacatctatg
     4741 attttttaca agcttacaca gaaaaagaaa cttacactgt taataaacag taccagtttc
     4801 gtctagataa acgggcgttt caaaaagaag atcaaaaaat actcacgcat cttgcaggaa
     4861 gtgctcagac gcatcaatta ttaggtacga acggcttaca agtcaaagga aaactagata
     4921 aaagatatct gcttctccca gtagaacaag cccgcttcct gttagagcag atgaatcaga
     4981 ctacacgttt gcatctgcag ataaatgaac aaaacgatca aggaatacaa ttttccgatc
     5041 aaaacaaacc tttatcattc aaagtggaaa aatttggaga agattatttg ctgcaagcat
     5101 tgaatgattt tgattttttc tttatgcatt atcagtgggg agttatccaa gggacatttt
     5161 attctctgac gaaatatcaa caaacggttt atcaaacttg gaaacaattg attcgccgat
     5221 tggagaaacc agaagtagtg ttcaagaaaa aagaattacc gtctttgttt aaagagatta
     5281 tccctctgct gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag
     5341 cagatatccc cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa atccaagctc
     5401 gagtcgattt cgtctatggt gaagtcatct attcgactga cccgaagcat gaggtctcga
     5461 cagaacaggc ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta cttgatctat
     5521 tcagaatgta tcgctaccag aaaaatgaaa ctggttatga acgtccattg ccaagcgggg
     5581 aagaactata tgcctttttc cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca
     5641 gacttggaag aaaattgcga gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg
     5701 aagtaaccga gaccggttca tggctcgata ttcgattcga tgtgacaggg atagaggagc
     5761 aagaaatcga tcatgtcctg caaagcttgc tgagaaatga tgctttttat acattagaaa
     5821 atggtcaggt tctttctttt gattcagaag aatttcaaca aacaagccaa gtgttgcaac
     5881 aattacggga atcgatacgt acagaagaag gaacgatcca tgtacctaaa aatcaaggat
     5941 tgatcattca aaatcaattg gaaaaaagca atgctacttt tagtgaatcg ttccaaacga
     6001 tggtacaaga tctcattcat ccagaaagat accaggcaca gcttcccaaa ggattgaatg
     6061 caacgatgag agattatcaa aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc
     6121 agtttggcgg tattttagcc gatgaaatgg gacttgggaa aacactacag acaattgcct
     6181 ttttactttc ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga
     6241 gcttaattta caactggcag gcagaggtga gaaaattcgc cccttcttta agtatccaag
     6301 taatcaatgg gaataaaaaa gaacgtgaag aattactggc taaagacaca gatatccgag
     6361 tgacttctta tgccagctta agacaggatc tagctaatta tcagtcgcaa aaaattgatt
     6421 atttgattct tgatgaggca caaatggtca aaaatagcag tacgaaaact gcacaagcat
     6481 tgagagaatt agctgtacct cagcggtttg cactgagtgg gacaccaatc gagaataatt
     6541 tagaagaatt gtggtcattg tttgcaacga ttatgccagg atttttcccg actaaaacaa
     6601 aattcaggga acttgccgca gaagaaattg cgcaaatgat tcgaccgttt attttacggc
     6661 gagataaaca aactgtgttg aaagacttgc cggaaaaaac agagatgaat ctatattctg
     6721 cacttacaga agaacaaaaa actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa
     6781 tcacatcaat ggattctgaa gcattcaaga aaaaccggat cgggatactt gctggtctga
     6841 caagactgcg acaaatttgc tgcgatcctc gtctgtttat tgaagattac caaggcggtt
     6901 ccggcaagct cgaacaagta aaagatctac tggtagctgc caaagagaac aaacgaagag
     6961 ttttgctgtt ttctcaattt acagggatgc tgactatctt gcaagaagag ttagccgagc
     7021 tagggatctc gacattttat ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg
     7081 tagacgcatt taatgctggc gaaaaagatg tttttctgat ttccttaaag gctgggggaa
     7141 ctggattgaa tctcacggga gcagatacgg tcatcttgta tgacctttgg tggaatcctg
     7201 ccattgaaga acaagctgct ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag
     7261 tgtggcgtat gatctctgaa ggaacgattg aggaacgcat ggattcactc cagcaagaaa
     7321 aacgcgaact gttccaaaaa gtgatccaag gtaatgaaga acagttgacg aaactgactg
     7381 aagaagacat ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa
     7441 gcgttcagta atgggtaaga aagcctttct aaagaagcga ttgtttattt gatttaaaaa
     7501 atagcaagga ggctgggatt ccattttatc ccagcttcct tgctatcatt tttttatttc
     7561 tatttttaaa ggttatacaa agattgtctt cattgtctct aacagctgat caaaagaaga
     7621 aatctcgata tcgcaaattg gagtctgttc aattttgcgt tctcgatggt tgaaccaaag
     7681 cgcttgccag ttagcacttt ttgcacctag aacatcattg tcgtaattat cgccaacgta
     7741 taaggtctct tctggaagca tatgaaattc tttctcagcc agctgaaaaa tctctttttc
     7801 tggtttttgg aaacctgttg cttgagaaat gagcatattg cgggaaggaa tccaattgtt
     7861 caattgaagt tgcttcagtt tttttgtctg atggtcagtc ggcccatttg tgataattcc
     7921 taatgggaca ttcttttcct tcaagaaatc gagggttttt ttgacctcct catgcaagca
     7981 gatattatca agttcttcct cgtaaatttt ttgaaataag aggccatctt cttccgtaat
     8041 atgagggtag tctaaatctt ttagtgattg gctgatccga tgtgctcgca tatattctag
     8101 tgtccaatct cctgccatta cttttgggaa gttttcatca ctatgatggc ggaaccggat
     8161 atatagtgga tgcatatctg catctgaaac aagcggaacg actcttttga ccgcgtttcg
     8221 aaatggctgc tgctgatcat acatcgtatc atcaacatca aacacaactg atttaatcaa
     8281 aattttacac gcctttcggt tttcattgat tttttcacaa atccttgact attgtaacag
     8341 aaaaaattgt ctaaaaaagc tgataaataa aggtttttct tactatattt ctatttcctt
     8401 gacagaataa ggtttttata tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata
     8461 agtttgatga acaaattaat cattatggta gaagtcgatt gatttatttt aatgcaatct
     8521 ctgtggaatt ttcgaaagat ttattttttt aatacagtct caatataact attcaaacat
     8581 aaaataacgt tatttgattt atttgtttat agtaaatttt atttagtaat cattgcttta
     8641 aaatactacg cttatataac agagaaaata agcgttataa taaagaacag tattatttaa
     8701 aatgacagaa aacattacta ttaaagaaat aaaaaacata aaaaatctta agaaatcggt
     8761 ttcatttagc ttttgagata aaataatagt acagttttat acgctgtttc atatgtttag
     8821 aaaatttgaa ttagatttga aaactctaat ttttaaatga taaaaaaagc gagaatgctg
     8881 gttttactgt atattaacat tagataactt tcattttttt aaaaaaatta tgaaaaaaag
     8941 gtagctattt aaaagttgat tatgtataat agaaacagat gggagattat tatgatcttc
     9001 tgttacaaaa aagtgattct tatcacgaaa aggagtggtg cacgttgtta acattatata
     9061 cttccccaag ttgtacttcc tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc
     9121 catttgttga acgtaatatt ttttcagaac cattgaacag ttctgaatta aaagcgattt
     9181 tacaaatgac agaagacgga acagaagaaa tcatctcgac acgttccaaa gtattccaaa
     9241 aactgaacat ggatttagat gatctgccgt tacaagaatt attggaactt gtgcaaaaca
     9301 atcctggatt gcttcgccgt ccaatcatga ttgatgataa acgtctgcaa gttggtttta
     9361 atgaagatga aattcgtcgg ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag
     9421 cacaattgat ggctggatta tagaaggata atacgaaact tggaaaag
//
LOCUS       DACCCR010000093         9434 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCCR010000093 DACCCR010000000
VERSION     DACCCR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.100"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            75..1379
                     /locus_tag="IVK93_002372"
     CDS             75..1379
                     /locus_tag="IVK93_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6387622.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1471..1836)
                     /locus_tag="IVK93_002373"
     CDS             complement(1471..1836)
                     /locus_tag="IVK93_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6387623.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1833..2198)
                     /locus_tag="IVK93_002374"
     CDS             complement(1833..2198)
                     /locus_tag="IVK93_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6387624.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2390..2638)
                     /locus_tag="IVK93_002375"
     CDS             complement(2390..2638)
                     /locus_tag="IVK93_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387625.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2637..2765
                     /locus_tag="IVK93_002376"
     CDS             2637..2765
                     /locus_tag="IVK93_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP6387626.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2822..4516
                     /locus_tag="IVK93_002377"
     CDS             2822..4516
                     /locus_tag="IVK93_002377"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP6387627.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4598..4789
                     /locus_tag="IVK93_002378"
     CDS             4598..4789
                     /locus_tag="IVK93_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387628.1"
                     /translation="MDLLPQMQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKVV"
     gene            5167..6300
                     /locus_tag="IVK93_002379"
     CDS             5167..6300
                     /locus_tag="IVK93_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP6387629.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6331..6657
                     /locus_tag="IVK93_002380"
     CDS             6331..6657
                     /locus_tag="IVK93_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387630.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKRHEI"
     gene            complement(6679..7143)
                     /locus_tag="IVK93_002381"
     CDS             complement(6679..7143)
                     /locus_tag="IVK93_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6387631.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7272..8521)
                     /locus_tag="IVK93_002382"
                     /pseudo
     CDS             complement(7272..8521)
                     /locus_tag="IVK93_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8550..8867)
                     /locus_tag="IVK93_002383"
     CDS             complement(8550..8867)
                     /locus_tag="IVK93_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6387632.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8893..9213)
                     /locus_tag="IVK93_002384"
     CDS             complement(8893..9213)
                     /locus_tag="IVK93_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6387633.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         3043 a         1860 c         1684 g         2847 t
ORIGIN      
        1 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
       61 tttgcaaaac cactttgctc aatgatatca ataacataaa aaaagaattt gaagaacaga
      121 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      181 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      241 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      301 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      361 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      421 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      481 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      541 aaatcttatt gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      601 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      661 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      721 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      781 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      841 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc attcgaacgt gcgctgattc
      901 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttc
      961 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1021 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1081 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1141 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1201 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1261 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1321 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1381 ggactgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1441 aatctcttga tacacatcag gctcacaacc tcacttattt tggacatcta cgggtccgat
     1501 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1561 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1621 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg
     1681 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1741 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcaaa
     1801 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1861 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     1921 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     1981 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2041 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2101 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2161 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2221 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2281 tctaatgata acggttcgct taaatttttc tttttcactc tccccttttt ctccctccat
     2341 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2401 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2461 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2521 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2581 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2641 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2701 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2761 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2821 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     2881 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     2941 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3001 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3061 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3121 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3181 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3241 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3301 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3361 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3421 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3481 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3541 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3601 agatgaaatc actgtgactg gtttgaaaat gaccaataca ctgactcaag aagaagaaca
     3661 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3721 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3781 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3841 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     3901 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     3961 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctatcg tcgtgcatca
     4021 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4081 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4141 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4201 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4261 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4321 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4381 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta tccctatgtc
     4441 acgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4501 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4561 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4621 agcttttgaa caccgtttac caagaggttg tgaaaaagct gccctgcatc aagtaataag
     4681 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttggacttat taccgcagat
     4741 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa gtcgtttagt ttcgagtatg
     4801 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4861 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     4921 cattttttca acgtttactc tcttgccaat ccaacatcat ctaacagata caactatttc
     4981 aatgattacc attacactaa aaaatcggaa actcacctct ccgaacacaa atacataaga
     5041 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5101 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5161 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5221 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5281 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5341 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5401 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5461 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5521 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5581 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5641 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5701 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5761 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5821 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     5881 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     5941 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaaacgg cacaatctct
     6001 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6061 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6121 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgaaat gattgcagaa
     6181 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6241 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6301 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6361 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6421 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6481 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6541 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6601 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa agaggcatga gatttgaaaa
     6661 aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag cggtcgtccc
     6721 actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg
     6781 atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc tgatttccgc
     6841 taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca gggcacaaag
     6901 atttggtgtc aaatcagggc ggttatggaa gtcgttttca ataacgcccg ctcctccgac
     6961 gatggtttct ttttcatcta agaccacata ccattgagga atactttcct tattagcgat
     7021 tccagtacga atactttcct catacacaac agcaggaact ccccattttg atgaaaacca
     7081 cttcgcactt tgttttacta attctgggtg ttctcttaat ttgatccagt tatatgtaac
     7141 caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag tctttatgaa
     7201 aattagcaat gacaacaata gtgatgtaaa aaaaacggaa aacaatcact tgttctccgt
     7261 tttttgatta atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg
     7321 acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct
     7381 gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata
     7441 acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aactaagacc
     7501 attggtgtca aaataaatgg aatgaacatg atcgggttca acacgatagg taacccaaac
     7561 atcgttggtt cattaatatt gaagacacct ggcgcaatcg acaagtttgt tacaaccata
     7621 tatggtttgt ttttacgtcc tactaaataa atagctaaca gtaaacctaa agttgcacct
     7681 gttcctccta agttgacaaa gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta
     7741 tgtccagctg cgattgcatt aacgtttgct tcaatggctg gcgcattgat tgtttgcatc
     7801 aaaggatcga ccatattcgc tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat
     7861 gcaagcagta aggcagatgg ataagagttt gcaagtccca taaatggttc ttggaccact
     7921 gtatagaaag aacctacgat atcatcaaca ccgaatgcag caaaaattgc agcaactaaa
     7981 gcaaacgcag agatagtgat cattgctggt agtaatgaag caaatgcttt tgcaactgcc
     8041 ggtggcacac catcaggcat tttgatgatc aatttttcat ttccgcttaa acgtacaaag
     8101 atttctgttg aaataatccc aacaataata gcaataaaca aaccgtttga gctcatacct
     8161 aatgctccgc ctaaagcgaa gaaagagcct aaagaaatca cacctgcgga aacaccgtct
     8221 tttcggtagc cgttagctaa atgataagca agaaagcaat caagacagaa agaatggcaa
     8281 atgtaccgtt ccaagcagca gccccgaatc ctttccatga ttcaccacca aaaattgagt
     8341 tcatcaattc ttggaacccg ggaatcggta agttattgat cattactgct aatgctccta
     8401 agatcattaa cggcatactc accataaatg agtcccgaat cgcaactaaa tgccgttgtg
     8461 caccaatttt tgatgctaca ggtataaaat gtttctccat aaatgcaaca aatttatcca
     8521 taaatcttcc ctcttttctt tactgtagat tacgcgttca atcgacgata cagatcaatg
     8581 atctcttttg ctaaatctgt aaaagctatg gatgtcatca gatgatcttg actgtggact
     8641 gtcaaaagcg tgacttcaac gtgatttcct tgtgcttctt gtgtcagcat ctctgtttga
     8701 gagtgatgtg cttggactaa agattcctct gcatctttga ttttttcatc tgctaaagtg
     8761 aaatcacctt ttttcgcagc agcaatcgct tccattgcat cgctttttgc atttccgcca
     8821 aacatgatca gacccatgat ggcttctaga tttttttgat cttccattta ttgtatcctc
     8881 cgataatttt tattatttgt ttattagttt ttctgcttgg tcgagtactt tttcaccatt
     8941 catcatgcca tagtcagcca tattgattat atctaaagga atgcctttag gtgctagctt
     9001 ttcagcaaat tgattttgca taaagcgtac ttgcggtcct aaaagtaaaa cgtctacatt
     9061 tttagattct agttgattat ccgcatctga agcagataca gcaaagatgt ctgcttccat
     9121 tccttttgct tctgctgcct tttgcatttt tgtgactagg agacttgtac tcattccagc
     9181 tgaacataca agcatgattg ttttcttttc cattttaaac acgttccttt ctatacttac
     9241 acttgtatat aagcaagtag cgtgccaact ttgaaagcgc tttaaaaagt tgatatcatc
     9301 acatttaagg tctataccaa gttgcataca cactaaaaaa gaaagtgcac actaaacaca
     9361 caaattaatt tttattacct tttttctttt aacgtgcaca aaaaaagttg tgacacaaaa
     9421 cttgtcacaa cttt
//
LOCUS       DACCCR010000094         9306 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCCR010000094 DACCCR010000000
VERSION     DACCCR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.101"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..2122
                     /locus_tag="IVK93_002385"
     CDS             <1..2122
                     /locus_tag="IVK93_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387634.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="IVK93_002386"
     CDS             2119..2739
                     /locus_tag="IVK93_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387635.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            3421..3801
                     /locus_tag="IVK93_002387"
     CDS             3421..3801
                     /locus_tag="IVK93_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP6387636.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3783..4697
                     /locus_tag="IVK93_002388"
     CDS             3783..4697
                     /locus_tag="IVK93_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6387637.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4937..5626
                     /locus_tag="IVK93_002389"
     CDS             4937..5626
                     /locus_tag="IVK93_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387638.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5747..5833)
                     /locus_tag="IVK93_002390"
     CDS             complement(5747..5833)
                     /locus_tag="IVK93_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP6387639.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6478..7215
                     /locus_tag="IVK93_002391"
     CDS             6478..7215
                     /locus_tag="IVK93_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP6387640.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7243..7782
                     /locus_tag="IVK93_002392"
     CDS             7243..7782
                     /locus_tag="IVK93_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP6387641.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(8280..8741)
                     /locus_tag="IVK93_002393"
     CDS             complement(8280..8741)
                     /locus_tag="IVK93_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387642.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            8874..9248
                     /locus_tag="IVK93_002394"
     CDS             8874..9248
                     /locus_tag="IVK93_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAP6387643.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3593 a         1414 c         1450 g         2849 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     1441 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggagcttgcg cccctttgac aagcggttat ttgcactaaa tacgtaggta
     3301 tttggctagc aaatatagag cttgccaaac ccttttagtg agctttactc attgtacctt
     3361 tttaggtaca atggtattgc gaacgatacg tccgtaggac gtactgaaag ggtgggactt
     3421 atgagcgaac aaaatcaaaa tttggctagc aattcttcta aaaaatatac ctatcgatcc
     3481 gagccaaaac aaatcagttt tcgagtgagc gaatccgaat ttgcaaagct gaagcaatcg
     3541 gctgaagctt tgcaaatgag tgtgccggct ttcgtgaaag ccaaggcaca aaacgcgcgt
     3601 ctggtgacac cgaaagtggc gccagacatt gcccaggcaa tcgcgcgcga tttagccaaa
     3661 gctggaggga atatcaatca aattgctaag tggtgcaata cacaccaaca tgacgttgct
     3721 tctggtgacg ctcaacgttt atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag
     3781 atatggcaac aattaaggta agccgaacca cttcttgtag tcgtgcaatt aattacgcag
     3841 aaccacgagc aaccattaaa acaggaataa actgtgacgt cgattatgcc aaaagtgaaa
     3901 tgaaacaaat tcgcatgctg tatggaaaag acgaccgtgt acaggcacac ttattgattc
     3961 aatcttttcg accaggagaa attaccgctg aaaaagctaa tcaactaggg gcagagtatg
     4021 caaaaaaaat agcccccgat caccaaatag ctatttacac acatacagac aaagaccata
     4081 ttcataatca cattgtcatt aactccgtta atctggagac tggaaaaaaa tttcaagctc
     4141 atggtcaagc atttctaaac aaatgttatg acctaaatga cgagatttgt cttgcacata
     4201 acttaccaat cactgagcga ggaaagaaac cagaaaaacg aaccatgtct gaaataaaat
     4261 taaaagaaaa aaatgaacct gtttggaaag acgaaattcg gattgcgatc gatcaaacaa
     4321 tgaaaaacaa aaaaaccaga acctatgacc aattttgtaa ttccctcaaa acattgggga
     4381 ttcattgctt caatcgcggg aaaaatttca cttacgaatt gatccacaaa aagaaaaaag
     4441 tacgatccaa cagactagga aaggactacg aaaaggagac gattttacat gagctggaca
     4501 gacgagaacg aacctataac caacagcgaa ccaacgagct acgaagagct cgagctagca
     4561 ttcgcgaaac tgcagaagaa gtacgaagag aacaacgaac tcttacgcct gaacaaccgg
     4621 aaattagacc ggctcgaaga aaccatgaac aacacgttga aagaggaaca gaccaccaac
     4681 aagaattatc gctctaaaac gttaaaagac atagaaaacg aaataaagaa acaacgtgct
     4741 tttctcgttc cttatacgca agaaaggata caaactacga ttgatttaca agttaaaaaa
     4801 ctccatcaag agctattaga ccagcgaaat aaggaacaga aaaaggtgct tattcaacga
     4861 tcattggatt ttcttgcccc cttatgttgc ttagtcctga cactgttcgc attttggtta
     4921 gtatggggag gctaaaatgg cattttattt accctatcta ctcatttttg ttagtatttc
     4981 aggaagtatt tggctcatct ataaaatttt tcaaacaaga tattcattaa aaggctctaa
     5041 aattcgattt aagcgatttt tctttcttgg ctgtatattt tccttgataa tcgtttcaag
     5101 cggcttattc ggcgttttag agggaaataa gcgcgtttct agaagtattt tattaggaaa
     5161 tgtcactcaa aagtatgaat cagcgcgtga taaaaagaaa aaagagcaag cattagcaca
     5221 aaaaatggaa gagtttacca cttgttatga ggaaatgaac gatatttttg tgaatcaaga
     5281 aaaacgtttg acggataaaa acatggaaaa actcactcgt ctttatcaaa acttgccaga
     5341 aaaacagcaa aaagaggttc aagacaacta tgagcgaacc aaaaaagacg tgcaatatgt
     5401 aaaagataca aagatagaag aagcctgtag tgatctattt ggagacacaa acccttggtt
     5461 tgccagtgaa gaagaaaaaa aagaaaaaca acaatctgtc acatatgaac gctatgaaat
     5521 cttacttcaa caagcaacta acatgcaaaa tccgacaaaa aaagaaaccg ccttaaacta
     5581 tctaaaaagt gtcaaggaat ggttagatca gcaacaacaa aattgaattt gatttaaaat
     5641 catgttaaac tgaatgtgca aatgatcaaa cattagatac acgaaagccc cagagaggtc
     5701 ggattctctg gggctttttt tcaggttgat gtcgccttta ttgattttac ttcttgtttc
     5761 ggttattcaa ccaataactg aataaagaga cacaaactcc tacaagaagg gggcaaatga
     5821 acagttcaaa cattagatac acctccttcc agtcgcaaga ctgtcggata ggcgacaagt
     5881 tattatatct taaaaaatta aatttgtgtg gtgtttttaa gtatcacttg ttttagggtt
     5941 gttagggctt gccctgaccg cctgtaaggc gcttctcgac tagattaagg gaaattaatc
     6001 aagaattgtc ttagaacggc ttagacaagc tttcagagtc tcacacctcc tctttgaaca
     6061 acggttttag caaaggagcc ggaggcgact gcgcagtata atatgagagc atagcgatca
     6121 caccttttta gcgaccgtag ggagcaagtt ttccagttta gaaaaattcg cgaacctttt
     6181 tggaggaaaa agtaggagtt ttttaggcgg gaaattcaat gtgctcttaa gatatactgt
     6241 tagatatact gttagatata ctgttaataa taactgttag ggattagcaa ccttatggga
     6301 gagtaagtat tgacataggt agtgggggat aaattgtcac actatggggg ataaattgtc
     6361 acactatggg ggataaattg tcacactatg ggggataaat tgtcacacta tgggggataa
     6421 attgtgtttt acatcctctt atgtctatga taacataatt tattgaggtg atttttattg
     6481 agtaacgata tagttcgata taataatgga ttaaattctg tccctttaag aaaatttacc
     6541 cctatagata tggatttatt ttggggaata tgttctaaaa tgaaaagaaa aggtacaaaa
     6601 gaagaaattt ttacatttaa acaaataaaa gaattaattg attacaatat aaatgcacgt
     6661 ggcgaccgtt ttgctaatga tttaataaaa atgtcaaaaa aaatcattac tttgtctttt
     6721 atttatgaag acgaaaaaac atatgaacaa ttaaatctgt ttcaaaaatt taaaatcgat
     6781 aaagtgaatg aaacattatt aataaaggtt tctgaagaat ttgaatatat acttaattca
     6841 atcggaacta attttactcg tttcgaatta gaaaatatga caaacttaaa gagtagctac
     6901 gttaaagagt tatatcgcca acttatggct cataaagaca gaagatctaa aaaaggtcac
     6961 tggtttgtaa aaatagaaga ttttagagct ctattagcaa taccagaaag ttatcgcatg
     7021 agagatattg ataaacaaat ttttgcccaa gcgaaaaaag aatttttaaa cgttaattca
     7081 actcaccctg ctatttttaa aacttttgaa atcaaaaaaa ttaaagccta caaaggaaat
     7141 aagataggat ctctgcaaat ttattttgaa gaaaatatac cttcaattac cttagataat
     7201 tggttagaga aataatacct aacaatttag gaggcaatta tcatgcaacc caaagaaacc
     7261 tttacaataa aggaattatc tgaattattt gaaataagta ggcaagcaat ctctaaacat
     7321 gttgataaat tagacccgtc cttattagct aaaaatgagc gtggttataa ggttgtatta
     7381 cgttctggag tattgcaact tgctagcaac ttaggtaacc aaaggttact atataggttg
     7441 catgagtatg aacaagaaaa cgataaaaac gtttatatat caagcaaaaa cgtgattgta
     7501 aacgagttgc tagataagtt gcgtgaagaa aatgaatttt tacgagaaca actttccctc
     7561 aaagagcaaa ataattctaa tatgcaaaaa ttactcgacc agcaacaaca gctcacccta
     7621 caggcaaatc aacagatcga aaaactgcaa gaacagttac aattagctta tacggaagaa
     7681 actccagaag atgaatcaac tactattctt tctgaagaaa cagaatcgat agaaaaacac
     7741 acaaaaaaga agaagcaaaa atggtggcaa ctttggaaat aactggaggg aacattcatg
     7801 acaagaaaaa atcttctatt cggttctatt ttactaggaa ttctaggctt cagtccgatg
     7861 ttattgtcag taatcaataa agggaataaa catggtgaat tgaaattgaa tccaaattat
     7921 tacaacagtc ctaacgaaga attgtttgta aaagagacgg ataaataata aaaaagactg
     7981 ctcttccgag cagccagtta aaaaataatg agtgtttatt cttgaaaaag cacgcgctta
     8041 tgaagcgcta ccaaaaattt accatatttt acaaaaaata acaagtctta aaaaaatgac
     8101 agataaaaat gcacaaagaa ttagatatag ataaccgcga aaaatcaagc tttagaaagt
     8161 tgcttttagc tagaccaatt aaaaatcaca aagtgagcgt ctctatccat tggtagaggg
     8221 gctacttggc atagtcgaca ttatgtcaac tatgcaatta aataactaac tacttcatat
     8281 tagcttaata aaataatcaa aataactagg gggaaaaaca ttgtataaaa aaatttttca
     8341 ttttttaaat aagaatagta aatataaaaa taatttaaga tatctattac tattaatcca
     8401 gatagagcaa accaagtaaa tattccatgt gtaaagctaa aaaaattcat ttgaatcaat
     8461 tcaaataaac ctaatatagt aacaaatata ttaaaaataa tagctgtcaa taccagtaaa
     8521 ttattttttt tattttttga attatcttca tttaaaatag aatttatgtt agtactataa
     8581 aattcacaaa ttttttttag tgtctctata tctggtgttg ttttgttatt ttcccacttt
     8641 gaaatagttt gacgtgtaac gttaaatttt cttgccacat attcttgagt ataccctttt
     8701 ttcactctta attctcttag cttagatcct aaatcctcca ttttttctcc tatgtaaacg
     8761 attatttaca taaaatttta tcataaaaat actaacgcgc caaaaaagtt ctttatttta
     8821 taaaacacac taaaataaaa taattctagt aaaaaagata ggagaaataa gttatgaaaa
     8881 aatttttatg tctttctgct ttaatgggtg tcttattaac tagtggagga atagctagtg
     8941 ccacagaagc tttaaatcta gatgtacccg aagaccatac cgccatttac ggaggaggaa
     9001 tggaagctac agaagaagga ttttcttcta gtaaattgag atatgctgct ggcggcgggg
     9061 atttcaactg tggggttaat ggatttaaag tttatgcaaa ttactaccat gctagagcta
     9121 aacatagtgc aacagctaaa aatggacgag gcggtcaagt acgatcagtt cagaaagctg
     9181 gagtaagagc ttatgcaaca tgcaatgcca cacttactgg aaatactggt tggtggaatg
     9241 tttattaaaa aagagagttt aaactctctt ttttatttac atttgaaagg aaattcaaaa
     9301 tatgtt
//
LOCUS       DACCCR010000095         9280 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCCR010000095 DACCCR010000000
VERSION     DACCCR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.102"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            119..448
                     /locus_tag="IVK93_002395"
     CDS             119..448
                     /locus_tag="IVK93_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6387644.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            438..725
                     /locus_tag="IVK93_002396"
     CDS             438..725
                     /locus_tag="IVK93_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6387645.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1011..1868
                     /locus_tag="IVK93_002397"
     CDS             1011..1868
                     /locus_tag="IVK93_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6387646.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1869..2417
                     /locus_tag="IVK93_002398"
     CDS             1869..2417
                     /locus_tag="IVK93_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387647.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2677..3310
                     /locus_tag="IVK93_002399"
                     /pseudo
     CDS             2677..3310
                     /locus_tag="IVK93_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(3355..4304)
                     /locus_tag="IVK93_002400"
                     /pseudo
     CDS             complement(3355..4304)
                     /locus_tag="IVK93_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            4906..6750
                     /locus_tag="IVK93_002401"
     CDS             4906..6750
                     /locus_tag="IVK93_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6387648.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            6806..8272
                     /locus_tag="IVK93_002402"
     CDS             6806..8272
                     /locus_tag="IVK93_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6387649.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            8309..9151
                     /locus_tag="IVK93_002403"
     CDS             8309..9151
                     /locus_tag="IVK93_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6387650.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         3185 a         1422 c         1874 g         2799 t
ORIGIN      
        1 gttgaaattt attcagcaag gacgcattac gagtcacagt aattacttgt aaatacatta
       61 tctcgtggta taatgtattt actaagtaat tacatactgt tagaaatgag ggatttaaat
      121 gattacaaaa acaagaaaac aaggcaattc aatcatgttg acagtaccta aagattttaa
      181 tgttcctaat ggagtcgaag ttgaagcaaa gttggtagag aatggtattc tttatgaatt
      241 tgttgaacca caaaaagaat tttatgattt tagcgaagat attttgtctg atataattgc
      301 agaaggctac gataaagatg agattcttgt agaatttaag aatagaaaaa acaaaatgca
      361 ttcttccttt agagatattg ctgaagatac acttactaat tctaaagtaa tgactaaaga
      421 ggagttggca acagaaattg gcttatgaaa tcgttccttc aaaacatgtc attaagtatt
      481 taaaaaagct aaaagagaag ccattgaaag aaaaattttt gaatttaatt tatgatgaaa
      541 ttgcgattga tccatataag ggtgaacaaa aaactgggga tttatcaggt atttggtcta
      601 gtgggttcaa atatgcgggc acaacatata gagtagctta cgagattata gaaaacaagg
      661 ttattccagt attgttatgt ggaacgcatg aaaactttta tgaacagttg aaaaaattaa
      721 tctagtgatt tgttgtcgtt cttaagaagg acggcttctt ttttatcgaa aatctgattc
      781 gtctacatga ctaaagtcgc gagtattctc tgcttctcga taaattaaat aacgtcatta
      841 ctttgaggaa agctataaat caatatattt cagcttttcc ttatttatgt actacctttt
      901 acttattaat tataaggtag tacatatata tctttttttt ggtagtaact aaaaataaat
      961 atgttataat agtatcaaat gaatattgct aaaagaaaag aggtaggaac gtggtagcga
     1021 agaaatatgt aatcggtaat tttaaaggtg gcgtaggtaa aagtacgtgc gcgcaaatgt
     1081 tagggtttga atcagcagtt aatgaaggaa aaagaacctt gataattgac ttggatatgc
     1141 aaggaaatac ttctgatgtg atgaacttaa cgcatatgaa cttcactaac gaagaaggag
     1201 gcggagaagg tgaaccgatt gtttacgaaa atactgtgac tgacgtgttg ataaacaatc
     1261 ttccacctaa agaggctgtc tataaagtaa tcaataacct ctatatactt ccagcagaca
     1321 tgtcctttga aatgtatgat gattggatca aagaccaata taatgattca ttatcccaat
     1381 tcaaatatat gaaagaaaag ctagaaccat tatttgatga gtttgacgtc atctatttag
     1441 atgtcccacc ttctatttct gtatatagta aatccgcgat gtacattgcg gattgggcaa
     1501 tcgtcgtctt acagacccaa gtaaaatcga tgagaaatgc gatgcaatat cttgagtata
     1561 tggacttttt tgtgaaagaa tttgatacaa atttatatgt tgctggtgtt attcctttca
     1621 tgttggaaag cggtgacgca gtagatcagg aaatgtataa acaagcccaa gagatttatg
     1681 gggaacattt gattaaaaat gttgtactca aaaatgcgcg cttaaaacga tacgacggat
     1741 caggtattac gatggagaag acaaagaaag gtctcttaaa acaatgggat cgcaaaagcc
     1801 acgaattatt tatcagtatt ttgaaagaat tggaagaaca cgagaattgg tatgcggagg
     1861 tggagtaatt ggctgacatc aaaggacttg atatttttgg gaacaacaaa ggactcgcca
     1921 aaataaaaaa agaagcagaa gcaaagaaaa agcagcaagc aattacacca tctgaaaata
     1981 agcaattaga agtgcaccaa gttcaagaaa taaaaacttc ctccaatgtt gaaagacaga
     2041 gtagaggaag aacaaaaaaa gttggcgcac caatcagaaa gtttgataag gtttattcca
     2101 ccaagcatcc tttaaaactg tcgactttgt taaatgcgac ttccagagtt ttagtagaaa
     2161 agtatgagac taatatgaca agagatgaat tgcttagaaa agctttagat gagtatatta
     2221 aacagaatct tacgcaagaa gataaacaag atttgtttaa tgacgtggtg aaagaactag
     2281 acttgttcag agaaaaatat ccaattgttc cagaattaga tgatcgtgga gaaataataa
     2341 gaactaccag tcagataaaa caagataccg aagactcact gcgcaaaggg tggggaatga
     2401 ataataaagt tagataataa tcaaagaaga actaagtaac tacctttaaa cggtagttac
     2461 ttagttcttc tttgatttgc gtgaaataga ggggatttat ttttctggtt acgtgattaa
     2521 aaaatccaaa aaatgagcat aggaacggtt atgattttag ggtgtgtttt aatgagatat
     2581 tgtgatttca ttaataagtg tatgataatc tgttgttgag ttagtaaata agtaatagag
     2641 ctaactctaa aataaaacta ttaggaggag atgtagatga aaaagtttaa aaagaaaata
     2701 tatttaggat tggttgttgg ggtgagtatg tttgggttca tggctcatac taaacaagta
     2761 actgcagata ctaaccaaaa agaaaggatt atgagccaat tagatgactc tcaaatagag
     2821 tatgtaccca atgagctaat tgtcagttta aaggctacac aaagaatttc aacagagaaa
     2881 aataaaccgc aaaatttatt gggtgtaaaa gttatggtgg aaaaagattt gactcttagg
     2941 acaaatgaaa gcgattataa taagcgagat caagtattat tagtgaaatt gaacgaagaa
     3001 attagtgtgg aagaggcagt aagaacattt aatgcttctg atatggtcaa ttatgcagag
     3061 ccaaactatg tggttgaact agagccgttg aatgatgttg aggagccaaa tacaactgat
     3121 tctttaggaa atcgataacg atcctaattg gagtaaacaa gctaatttaa gagagattag
     3181 catgccatcc gcttgggaag aaaccaaggg ttctaaggat gtgaaggtag cggtcattga
     3241 ttcaggaatt gattatcaac atgaagatct ttctggaaat gtagatgaaa gtttgggata
     3301 cgattttttt tacttttttc aagaaaataa taattcagaa tattctgact atatttatgc
     3361 aacactagtg actttaggag aaacatcaat agccttgcta aagtttttgc tttcgctaaa
     3421 gagagtattt tcgataattt gtttttttct aaaatacctc gttcctcaat atttgggata
     3481 tggatatgta tagggattct tcgcataaag gtttccagaa aatcctcttt gccttctgta
     3541 gtcgcaaaaa ctaaacgtac attggaggcg tgatggatag tttcaccgac tctagaataa
     3601 gttccgccat ccatataaac aaatagtttc tcttgccctt caggaggcaa tctgtgtact
     3661 tcgtctaaaa acagtactcc accgtctgca tcttgcaaca atccatttcg atcttcatct
     3721 gcgccggtaa aagcaccttt aacatagcca aatagtatcc ccgacaataa ttcaggatta
     3781 ttatggtatt gcgcacaatt aagtacttta aaaggagcat tctcatgaat aacgttcact
     3841 tttttcataa actgataagc agtatgaaca aaaaaacttt ttccaactcc agtatctcca
     3901 gtgatcatca taggaagccc tcttccagga tagaagacag cagttttcat ttgttccacc
     3961 gcatcggata agctcatatc atacccaatc atcttttgta gaatggaatc tgtagaattg
     4021 ggctccaagt ctgtttttaa ttcagatata ctttcgtata catcttttac cggaatcaaa
     4081 aagtgctctt caaagcgctc tttgatggca aataacactg gacgagtatt gattttaaat
     4141 agaacgttct ttgcaactaa gtcattggca agtttactgg catttgttct tcccgttctt
     4201 aaaaattgcg acaggtcatt tgtgtcaaaa aagcactctt ctaaattact gatgccaatt
     4261 tcttcacatt ttgtgtttgc ataagtcact atatcatttt tcatcttata aacagtcctt
     4321 tctaaacttc tatcccttaa ataaatagca aaaaaaagga agcaatatcg atatgctgtc
     4381 ctcttaattt aataaagcgc taatctaatt cactgtatta tcatttttta gtagtatagc
     4441 acaattatga atgccttttc tttatatcaa ataaagttta tataaaaaac ctcatcctgc
     4501 aagaaagaaa aaatgaaccc attcttttta tactaggtct taaaaagaca gaacatctgc
     4561 tagaagatag aagagaataa gagttatata tgttggaaga tattcttaga aaaaagacaa
     4621 aacttttaga acacgaaaac tatattgaac gaataaaatg aaaaaatgat taaaaaatca
     4681 ttttattatt tacgtttaca ttctaatatg ttaaattgaa tataacttaa acaggcatgt
     4741 gactggtaat gcaggcagga cctaaataag ttgaataaca atgtatgcgt ttcaatacac
     4801 gtttgccttg ttgtcaattt atttagtttc tgcctgtttt ttgtgttgag gatggccact
     4861 tcgactcaaa agttcatcac tcaaaaaaga aaagagggaa acaccatgaa ttacaaacaa
     4921 acagcagaag aagtattgca gtacatcggt ggggaagaaa acatatccca tttggagcat
     4981 tgctcaactc gtttacgttt tacgttaaaa gacaacagca aggtaaatat gagccagtta
     5041 gaaaaagtgg atggcgttat gggcgttcgc caaaatgtgc aatgccaagt aatcattgga
     5101 aacgatgttg ttgaagtgta tgatgaagtg aaagcgttat taggtgacaa tgtttcaaat
     5161 actgaagaag caccaaaaca aaaacaaaaa ttaggttcag ttctattaga ttttattatt
     5221 tctattttcc aacctttaat tcctgcgatt gcaggtggag gagtattgaa gtccttgttg
     5281 ctactagctt ctatcttagg gctgattagt gaccaatctc aaacctatca gattttaaat
     5341 ttggtcggcg gggcaccgct ttatttcttg cctattctgg tggcaatgac tacggctaat
     5401 aaattgaaag tcaatcaatt ggttgcagta tctgctgtcg gtgcattgat ccttcctgaa
     5461 atgacagcta tgttggcgga aggtgctaca ttcttatctt ttggattgga taatatagcg
     5521 tatgcatctc aagtattccc agccatctta actgttttat tttatgctca acttgaaaaa
     5581 tggtttacaa aagtttcacc aaaaccaatt cgtattttct ttgtacctat gatgagctta
     5641 ttgattacag ttcctattgc tttgttgctt ctaggaccat taggttataa tgtaggaaca
     5701 gctttctctt ctatcattgt gtctttgtac aatcaatttg gatgggttgc aaccggagtc
     5761 ttagcagctg tactgccatt aatggttgta acgggtatgc ataaagcaat gatcccttac
     5821 gctgtttcat ccatgagtga attgggtgca gaacttcttt atctcccggc ttcattggca
     5881 cacaatattt cagaagctgg cgcatgcttt gctgtcagta tcaaaacaaa agacaaaaaa
     5941 ttgaaatcta cggctgtttc tgcgggtata tctgcattat tcggaattac tgaaccagct
     6001 ctttacgggg taacgattct gcacaaacgt gttttatatg ccgttatgtt ttctagcctg
     6061 atcgggggag catttgcagg aatcgttgca cttcaagcct ttgctttagt gggtcctggg
     6121 attgcaagta tcacgatgtt cgctgacaaa gctgacccaa tgaatctggt ttgggcattt
     6181 gcagtatcgg ggatttcatt tgtggttgct tttgctgcag cgttggttct ttataaagat
     6241 aaagaagttg tcaatgaagt tgaaactaag atggaaacgg ccgttgtttc agatgctgtt
     6301 ctttcaagtc ccgtgaatgg aaaagtaatc tcactatcag aagtaaaaga cactgttttt
     6361 tctagtggta tggtaggtga tggagtagca atcattcctg aagaagggat attagtagct
     6421 cctgaagatg gagaaatcac aatggtattt gaaacgaagc atgctattgg tttaaaaacg
     6481 gctaatggtg cagaacttct ttttcatatt ggtattgata ctgttcagtt agaaggaaaa
     6541 gggtttgagg catttgttcg ttctggtgaa aaagtagcca aaggacaaaa gctgattgca
     6601 tttgatattg atttaatcaa agaagcagga ttcgacccca cagttatctg tgtggtaacc
     6661 aataaggata attacactat ccgttcattt aacctagaaa aattggtaac caatgatagt
     6721 gatctcttag ctatcagtcc gatagcttaa acacaagata gtgaaaaaaa atatattgaa
     6781 tgaaggaata gaggagaata gaataatgac aaacacaaca agttttccaa aaggattttt
     6841 atggggcggc gcgatagcag caaatcaagt agaaggtgct tggaatgtag acggtaaagg
     6901 tctttcagta gcggatatgg ctacttataa accaaattta gacaacaaag attttgctgc
     6961 tcatatgcat gtcaccctgc agtctataaa agaagctagt gaagacaaat ctgataaatg
     7021 gtacccaaaa agaaggggcg ttgattttta tcatcactat aaagaagatt tagctttatt
     7081 tgccgaaatg ggctttaaaa ccttgcgcct ctctattgct tggactcgct tgttccctac
     7141 aggagaagag ctggaaccga atgaaaaagg ggttgcattt tatgaaaacg tctttaaaga
     7201 aatgcaacgt ttaggaattg agccgattgt gacactatct cattatgaaa tgccattagc
     7261 tttagcagtt aaatataatg gctgggtgga gcgtcgagta attgatgatt ttgttcgctt
     7321 cgctaacgtt tgttttgatc gcttcggcaa atacgtaaaa tattggctga catttaatga
     7381 gatagacagc atccaccgtc atccatttac aacagctgga attatccaag agaaaagcgc
     7441 agaaggtcaa gaagctcaag atatctatca agcattgcat catcaatttg ttgcgagtgc
     7501 gttagtgaca aaagatgctc atgaaaaaat tccaggaagc caagttggtt gtatgttaac
     7561 gaaattgatg acttatccat taacttgcgc accagaagac gttgagctga ccttgaaaaa
     7621 gaacttagaa aataattttt atgcagatgt tcaagttaag ggagaatatc ctgctctaat
     7681 cgaaaaggat ttggaaaatc ggggaattca gattaaaatg gaagatgaag atcgagaaat
     7741 tttaaagaaa cataccgtag attttgtttc tttcagctat tacatgtcca tggcagaatc
     7801 agctgatcca aacgcagaac gcacaccggg aaatactgtt ttaggggtta agaatcctca
     7861 tttgccttca acagattggg gctggcaaat cgatccaaaa ggtttgaaaa tttcgttgat
     7921 cgaactttat gatcgttacc aagtaccgtt gatgattgtg gaaaatggta tgggagcagt
     7981 cgatcaggta gtagatggta aaatccatga tccgtaccgc atcgactact ttaaagcaca
     8041 cttccaacaa atgaaagaag caatcgatga aggtgtagat ttgatagctt atactagctg
     8101 gggaccaatt gatttagtta gtgcaggaac ttctcaaatg tctaaacgat atggatttat
     8161 ttatgtggat gcagacgatg aaggaaatgg gacatataac cgcttgaaaa aggattcgtt
     8221 caactggtac aaagaagtga tcgaaacaaa cggtgcatcc ttattcgagt agtctcgatc
     8281 tttttaagca gcgatagggg gataatgagt gatttcaata aaaaaagtat tgaattcaag
     8341 tgtggtactt gttgaagaaa atggcaaaga aatgattgca ttaggtaaag ggataggctt
     8401 tggacagaaa gctggggatg aaatatcagg agataaagta gataaaatat ttctgccttt
     8461 tgaagaaaag aaatcttcac aactattaga gttagttgaa gaaataccta ttgaattttt
     8521 tgaaatcact cgtactatcg tttttgaagc agaaaaagga ttagataaaa aactgaatcc
     8581 tggagtctat ttaaccttaa cagaccacct gcattttgca gttgaacgcg cacaaaaagg
     8641 ggtaaatatt gcaaaccgtt tgtactggga aataaaaatc tactatccca aagaatttca
     8701 aattgggaag tacgcactca cccttttgaa cgagcaatat ggaattgatc ttcctaaaga
     8761 ggaagcctca aacattgctt tccatttgat caatgcacaa tcggaagatc ctgatagcgg
     8821 cgctggattc aagtatgcaa aaatgatcgg cgggatcgtc aatttggtcc ggtattcgtt
     8881 gcagaaagat atcgatacta attcgatcca ttatgcccgc ttcattacac atgtacgttt
     8941 ctttgtagaa agatattatt ctgatgcttt gttggatgat ggtgaggaag aattctacaa
     9001 tcaaatgtgg atcttatacc cgtctgccat ggaaagcgca gctaagatca aagactacat
     9061 tgaaaaagtt tatcataaaa caattccgaa cgatgaagtt gtatacctag gtgtccacat
     9121 caatcggttg atgaaacatg ctaatgggtg agcgctcagt aatactattg atgcttttaa
     9181 aatctttaga gatggattat agtaaaaaca aggtgatttc cagctgtttg ttatctataa
     9241 gagacattta aaaaggatta acgtaagata ataaaaaatc
//
LOCUS       DACCCR010000096         9138 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCCR010000096 DACCCR010000000
VERSION     DACCCR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.103"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            634..1206
                     /locus_tag="IVK93_002404"
     CDS             634..1206
                     /locus_tag="IVK93_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP6387651.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            1438..2055
                     /locus_tag="IVK93_002405"
     CDS             1438..2055
                     /locus_tag="IVK93_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6387652.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            2283..2621
                     /locus_tag="IVK93_002406"
     CDS             2283..2621
                     /locus_tag="IVK93_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387653.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2606..3460
                     /locus_tag="IVK93_002407"
     CDS             2606..3460
                     /locus_tag="IVK93_002407"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP6387654.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3607..3840
                     /locus_tag="IVK93_002408"
     CDS             3607..3840
                     /locus_tag="IVK93_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAP6387655.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            4038..5942
                     /locus_tag="IVK93_002409"
     CDS             4038..5942
                     /locus_tag="IVK93_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP6387656.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            6296..6661
                     /locus_tag="IVK93_002410"
     CDS             6296..6661
                     /locus_tag="IVK93_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387657.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            6678..7289
                     /locus_tag="IVK93_002411"
     CDS             6678..7289
                     /locus_tag="IVK93_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAP6387658.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            7317..8978
                     /locus_tag="IVK93_002412"
     CDS             7317..8978
                     /locus_tag="IVK93_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP6387659.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPDVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLTKGFCCKVLNLLSNKVE"
     gene            9006..>9138
                     /locus_tag="IVK93_002413"
     CDS             9006..>9138
                     /locus_tag="IVK93_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6387660.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     "
BASE COUNT         2942 a         1478 c         1987 g         2731 t
ORIGIN      
        1 tagaaacgtc atcctttaca gaatattcaa taatgctttg aatctcttcg cttttcagtg
       61 taaaatgtac ttgggtcatg taaaagtcct cctgggtatg tttttgtcgt taaaaacatt
      121 gtaccgtaaa aggactgtta tatggccttt ttacttttac acaattatac ggactttatc
      181 ggtataccct aaatagacat cataataaca ttgaaaaaca tggttgtcat ttttctagat
      241 aaatggcata aattttcagc gctaagtgac aaaagcaact aatttttcat gagagatttt
      301 taaatatctc aatctatctc caaaattagt tgtttttgta gctatagttg actatgaata
      361 tgacaaataa ttagaaaaaa ctgtgccaat taagtataaa aatgacattt taggctctac
      421 tttaatgaaa tttttgtttg gagggaacga cttcattcct ttgattattt cagttgaatt
      481 taatagagaa gcctggcgtc tattaagaaa taattttctt gatgagcgtc aattttttta
      541 acggaaatct atcctatact aaattcagta aagaaaaagg aggaatcaaa gaaagctatt
      601 ttaaacaaac caaaattata ggaggaataa atcatgactg aaaataaagc tactcaagaa
      661 agaacacccc aagaaagatt gcaagaagaa caagaacaca aggaacatgt tcatcatacg
      721 aaaattaatg cagcggccat tgcggatcat cttttaggca acatgcatac attacatgtg
      781 aaactgcacc aatatcactg gtatgtaaaa ggagccaatt tctttacgtt gcatgaaaaa
      841 tttgaagagt tgtataacga aaatgaaaaa tggttcgaca aacttgcgga gcgtctaatt
      901 acttctgggc ataaacctgc ttcgacaacg gttgaatttg aaaaatattc gatgctttcg
      961 gaagatgcag ttaataaata cgctaaagca gaagacatgg ttgaaaatat tatcgaggac
     1021 ttcagaagca ctcgtgacct aactattcgc gcgattcgtt tagctcaaga cgagggtgac
     1081 gatgctttag aagatacatt gattgctttt aaaaatgact tagacaagaa catttggatg
     1141 ctgcaagcgt tcattggtaa agaagcatta gaagatgatg acgctcttga tgaggatgaa
     1201 gattaaggta aaagtagaag ctgatttagg gattggaatt ctttccagtc ctttttccat
     1261 tttcttgatg tgcatcaatt tttttgtagt aagagactta tataataaaa gcagcgaata
     1321 taaacaaaat aaataaaggg ggagaatgaa ttgactaaac atacccatca tgaaattggc
     1381 gaccacgcag cctgtatccg cttggttcca atattcaatc acttagagga tagcgcgatg
     1441 aattacattg ctgaaaaagc ccacacgaaa cagtatcaaa aaggagagtt tctgtttcgt
     1501 tcacaagaaa aagaggatac tttatatatt atcaatcgtg ggaaaatccg tatttatcga
     1561 ttggcagact ctggcaaaga gcagctagtg cggatcttga accctggtga tttcaccgga
     1621 gaatggacct tgttcaaccc ggatgcagta cacgaggaat atgccgaagc aacccgagat
     1681 accgttgtct gtatgattca gcaaaaagat gtccaagatt ttctggaaca atacccagct
     1741 atttctttaa aactattggc ggaaatgtct aaacggttag aaagttcaga acgtcaaacg
     1801 acacaagtag ccgtggagag tgtcattacc cgtttagttt tgtttttggc agaaaatgtg
     1861 gaacctgaaa tgggcaactc tcccaccatc accctgccga tggcaaaaaa ggacattgct
     1921 tcctatttag gaacgacacc tgagaccatc agtcgcaaat ttggagaatt ggaggacgaa
     1981 ggattgattc aacagctgtc tgggaaaagg atcaagattc aggatttaga tgatttattg
     2041 ctttacagcg aataatcgga cacacttttt tctacacgtt ggtgttctgt cgctcttcat
     2101 taacaacggg acagaactca gtgcttttgc ttccatctgt ccgtttttgg cttttttaag
     2161 acagaacgac cttcattcat tggaaaaata aaagcacaaa catgataacc ctcaagtttt
     2221 tttgttttat ttttctttaa gatagggttg tcgaacaaaa cgtaaaaata agtagggtga
     2281 atgtgcattg tttttgggtg atagttgcca tcacgttgtc agcagcgggg gcattctttg
     2341 tcgtcccttt caccaagaac acgattggcg gcgaagcaaa aacgtctaac gttgttgagg
     2401 cagataggtt agctatggaa aaagaagagg aagttgtaaa aggagccggc gtaatcggac
     2461 acggaaaagt ggcgtattac gaagcgagtg accagggaga taaaagtgta ttcactgttt
     2521 ggtttccatg gagcttagcc ggaatcttgt tcagcatcac catgactaga atgacgcgaa
     2581 aaattgaaga aatggagcaa atagaatggg attcgtatta ggcgttttga atccccatgt
     2641 ggtggagatt ggttctgttg tgattcattg gtatggagtc attattgcag cgggcatatt
     2701 agcggctctc cagttgagca ccaaggaagc aaagaaaaaa ggactggaag aggataccat
     2761 tatcgatatg gccttatggg caattccgat tggactcctc ggcgctcggc tttattatgt
     2821 gttatttgaa cttggttatt atctgcaaaa tccaggacaa atccttgcga tttggaatgg
     2881 cggcattgcg atttatgggg gattgattgc aggagggggt acgctatatt ggtatgcaaa
     2941 gaaaaaaggt acttctttgg cactcgttct tgatatttta gcgcccaatg tattgttagc
     3001 ccagtccatt ggtcgttggg gcaattttat gaatcaggaa gctcatggtg ggccggttac
     3061 tcgtcaattc ttggaaaatc tttatttacc tgaatttatc attgaacaaa tgaatatgaa
     3121 tggtacctac taccatccga cttttttata cgagtcattg tggagtttgt tgggctttgt
     3181 cctcatcgtc actatacgga accgaaaaca gcttttgcgt caaggagaag tcgctttgag
     3241 ttacgttttg tggtactcag tgggtcggtt cttcattgaa ggtatgcgaa cggacagttt
     3301 atggattggc gagtggatcc gcgtttcgca agccttgtct ctggccctgt ttattggcgc
     3361 gattgtagta tggtttatac gcagacaaga ttatccacca atttcttatt attctgatgg
     3421 caataagggg cagaaaaaga ctattaagat ggtgaattga gcaaaaagat aaaaagaagt
     3481 gcaaaacttg atatgcgtca attttcgttt gctaaaagtg agctatacta cagttgtaaa
     3541 gaggaaaagg cagtgttcat taatctgctg ccgataaaat aaaaacacat aaaggagata
     3601 ggaattatgg aaaaagctat attgcaatta gaaacgttgt cttgtccaag ttgtatgcaa
     3661 aaaattgaag gtgccgtgaa atcagttaac ggtattgata aagacagcat taaagtatta
     3721 ttcaactcca gcaaagtcaa aaccaatttt gattcagctg tcacttcgat tgaagagatt
     3781 caaaaagcta tcgaaaacgt aggctatccg gttcttaaag caaaagtcaa ggccgtttaa
     3841 cagattaaat gaactaggca attctggaag aaaagctatt tttccaggat tgcttttttt
     3901 gttgcaatcc gattcgaggt gcttagaaac gataaaagag aactttctaa acttgatgtc
     3961 agtcaattaa ttacttaggc aattatctta tactgtgttt atcaataaaa gagaaattaa
     4021 atttggaggg aaaaagcatg caacaatata tattaagcaa gaaaaatgtt atcaccgtca
     4081 tcagtggatt gttaatcgta ctcggtttct tcagtcactt tgttttagaa aacgtaggac
     4141 tttcagagtg gtctttgatc atcgcctctg tctttgggat tacgccaatt gccattcaag
     4201 cgtttcaagc aatgaaagtc aaagtcatca gtattgatgt cttagtcagt attgcagcga
     4261 ttggtgcgct ttttattcaa aattatgaag aatcggctat tgtcacgttc ttattcttat
     4321 tcggacacta cttggaacaa cgaacattga accaaacgcg atctgctatc aaagaattga
     4381 ctgaaatggc acccgaaagc gccttgaaac aaatggataa tggcgaattt gaagaagtag
     4441 aagtggatga tatagatgaa ggggatatct tgctcgttaa aacgggtgcg aaagttccag
     4501 tagatggcat agtccttacg ggtgaagggc atatcaatga agctagcatt acaggtgaag
     4561 ctattccggt caacaagcta gctgatgcag aagtttttgc aggaaccatt ttagaaaacg
     4621 gaacgattca aatcagagct gaccgagttg gtgaggacac cacatttgga aaaattattg
     4681 aactcgtgga agaagcacaa gattctaaat ccgaagcgga acggttcatt gatcgctttt
     4741 ctaaatacta cacaccagct gtcttggttc tggcaattgt tgtatgggcg ttcagtcaaa
     4801 atattgagtt agcaatcacc atcttggttc taggttgccc aggcgcatta gttatcggag
     4861 tgcctgtctc aaacgttgcc ggtattggga atggtgctcg taacggtgtt cttttaaaag
     4921 ggagtgaagt cattcaagac ttcagtaatg tggatacaat tgtatttgat aagacaggta
     4981 ctttaactgt cggaaaccca accgttgcag cgactgaact gtatgaaaaa gatcctgctg
     5041 aaacgcttgg ctatctggcc agtgtggaac gggaatcaga tcatccatta gcaaaagcgg
     5101 tcttaaatca aattggagaa acaaactttt atcctgttga aggaactgaa gttgtgaaag
     5161 gcggcggaat cgtttcaagt gtagctggac atagagtggc tgttgggaat gtggccttga
     5221 tggaaaaaga aaatgtcact ctcagcaaaa aagtgcaaaa agatgtcaaa cggtttgaac
     5281 aacaagggaa ctctctcgtt ttgacagcgg tcgacggtga attaaaagta ctgatgggca
     5341 ttcgcgatca agtccgtccg ggtgtgaaag ctaatttgca ggaattaaaa gacttgggcg
     5401 tgaaaaatct agtcgttctt tcaggagata accaaggaac cgttgatgtg gtcgccggcg
     5461 aacttggttt gaccgaagct cacggctaca tgttgccgga agacaaatcg gcttatatcg
     5521 gaaaacttca acaacgtggt caaattgtag cctttgttgg agatggcgtt aacgatagtc
     5581 cgtccttagc tttagcagac attggaatcg caatgggaag cggaacggac gtagcgattg
     5641 aaacatccga tgtcgtttta atgaactcga acttcagcaa cttgcctcac gcattaggat
     5701 tagtaaaagc caccgcaaat aatatgaaac aaaatatcgt gatttcagtt ggagtagtgt
     5761 tggtcttgtt gaccagcgtc ttcttcagcg aatggatgaa tatgtctatc ggaatgttgg
     5821 ttcatgaagg tagtatcttg gtggtaattt tcaatggcat gagattaatg aagtataagt
     5881 tgaaaggtcg gaaagaacaa aaagaagtat cagcacctac agagaaagta aaaatatctt
     5941 aagaataaat tagaagaggc tgagacaata gtccatcctt gaaatgacaa aagcggaaac
     6001 tcagattttt aaatctggga ttttccgctt ttttgagttc ttgaaaaaaa ataagagtat
     6061 agagtatgtt ctttataaaa ttaattttcg tgtaaattat taaaaattta ataataaaat
     6121 ttaaattcct attaagttgt aacacatgaa attgcttaaa atagtgtatc aaaaaccttg
     6181 ttttattaat gttgaattta tttgaaaggt ctacagatta acatattatt gacaaaacaa
     6241 tatttaaata gttatacttc attcaaacgt ctgtttgaac gaggtggtga agttaatgac
     6301 tgcagaagta tgtacaacta cttgtataca taaagaaaaa gtagctagtg ttaaaaataa
     6361 tttaaaagat aaagaccttt caactttact tgtattagga aaatgtttta gcgattcttc
     6421 taggattaaa attttctatg ctttagaaac gtataaggag atgtgcgttt gtgatttagc
     6481 agcgatactt aatgctagta ttgctacaac gtcacaccat ttacgttttt tgaaaaagaa
     6541 cggaatggcg aaatcacgtc aatatggaaa ggtagtttat tactctttag cgaatgaaga
     6601 gattctttcc gctgtgcagg cttttctaaa gttgtcagaa aatagttcta tgaaagctta
     6661 gtttggaggt agttattttg ttacaaaaca ttttatcagc actcgctgta tatatttcta
     6721 ccagtattga ttatttattt atcttgctga ttattttttc tcaaaaccat actaaaaaag
     6781 gtctccgtca gatttttttt ggtcaatatc ttggaacagg aatcttggta gccataagtt
     6841 tgtttgcagc atatgtgcta aattttattc ctcaagattg gatcattggt cttctgggac
     6901 taattccgat ttatttaggg attcgtgttg cttttgtggg cgaagaggag gaagaagaag
     6961 gggaagtcgt tgagaagcta ggatctagag gaacgaatcg tttgttttgg acagtttcct
     7021 taataacgat tgcttctggt ggcgataatt tagggattta tattcctttc tttacctcgt
     7081 tatctttttc agaaattgtc acttctttaa tcgtctttgc tatctctgtt gcggttcttt
     7141 gctatattag ttataaattt gcgaaaattt cttttgtctc tgaaacacta gaaaaatatg
     7201 aacgaatcat tgtacctatc gtatttattg gattagggat ttttattatg atagaaaatg
     7261 gaaccatcca aactatatta ggtttgtaga gatcttatta gtaaaggaag caaacgatga
     7321 gtgaaaataa aaagtaccat gaatggatat tagaaggaat cgactgtgca aattgtgcag
     7381 acaaagtaga aagaggcgtc gccaaagttg caggtgttgc caatagtagt gtgaactata
     7441 tgacagaaac cttgagtttc gaagtgacag aggaaaaaga atatcttgtt ttatccaatg
     7501 tgcaacaaaa agtaaaaaaa ttggagcctg atatcacttt aaaaaataaa gtggacggta
     7561 ggctgattga aataaatccg gcattaatca agaaaagtaa tttaaaagac gataaaaaaa
     7621 actacgaatg gatattagaa ggaatcgatt gtgcaaactg tgctgccaaa gtggaaagag
     7681 gagttgctac agttcccgac gttacaaaca gcagcgtcaa ttttatgaca gagacactga
     7741 gcttcgaggt agcaggtgaa aataaaactg acgtgttggc tagtgtaaaa caaacagtag
     7801 gaactttaga acctgacgtt gttctgcgaa aaaaatcgga cggtacattg attgaacatg
     7861 gaactgactc taataaaaat tcgacttcta acaaatcgat ttctgataaa gatgagacaa
     7921 accaaaagca gacgaataga gcttatctgg ccagccttct cggaaataaa actaaaatta
     7981 cccttattcg tttaatacta ggattaggaa tattagcgac aggaatattt ctccctatgg
     8041 atggacaagc ctctcttggg ttgttcattc tggcttattt aatcaccggt tatgacgttg
     8101 taggacgtgc cattagaaat attttccgag gtcaagtatt cgacgagaac tttctaatga
     8161 ccattgcgac gttaagtgcc ttctatattc aagagtatcc cgaagctgta gcagtcatgc
     8221 tattttatca agtgggagaa gtattccaag atattgctat ttcaaaatct cggcgctcca
     8281 ttgcggacct aatggatatt cgacctgatt atgccaattt agtcacagga aacaacgtag
     8341 aaaaagttgc tcctgaagca attaagattg gaaatattat tctcattcgt ccgggcgaaa
     8401 aagtgccatt ggatggaaaa gtagtggaag gtgcttctgc gatggacact tctgctttaa
     8461 caggcgaatc cgttccaaga agcgtgaagc cggaagacac tattttgagc ggatttatta
     8521 ataaaaacgg attgctaaaa gtagttgtag aaaagccctt ttcggagtct actgtgaaga
     8581 aaattttaga tttggtccaa aatgctagtg gccggaaagc acctactgaa caattcatta
     8641 ccaaattttc tcgctactat actccaattg tggttatagc tgctgcctta ctggcaattg
     8701 ttccgccatt gctagtacca ggcgctactt ttaatgaatg gatatatagg gccagtatct
     8761 ttttagttat ttcatgccct tgtgctttag tcgtttcgat tccagttggt ttctttggtg
     8821 ggattggttc tgcttcccgc aaggggattt tagtaaaaga aagcaatttc ctcgagggtt
     8881 tgaacgatct taaatacgtg gtaatggaca aaacgggtac attgacaaaa gggttctgtt
     8941 gcaaagtttt aaatctacta tcaaataagg tagaataata gaaaaagata gtaggaggaa
     9001 tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga tgtgattatt gtagccgtgg
     9061 gatactatct tcgttataac cttagctatc gtgaagttca agaaatctta tatgatcgtg
     9121 gcattaacgt ttctcata
//
LOCUS       DACCCR010000097         8838 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCCR010000097 DACCCR010000000
VERSION     DACCCR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.104"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..626)
                     /locus_tag="IVK93_002414"
     CDS             complement(<1..626)
                     /locus_tag="IVK93_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAP6387661.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            complement(849..1880)
                     /locus_tag="IVK93_002415"
     CDS             complement(849..1880)
                     /locus_tag="IVK93_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387662.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(1887..2717)
                     /locus_tag="IVK93_002416"
     CDS             complement(1887..2717)
                     /locus_tag="IVK93_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387663.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAKTGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(2714..3520)
                     /locus_tag="IVK93_002417"
     CDS             complement(2714..3520)
                     /locus_tag="IVK93_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6387664.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DILSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(3526..4350)
                     /locus_tag="IVK93_002418"
     CDS             complement(3526..4350)
                     /locus_tag="IVK93_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP6387665.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(4340..5440)
                     /locus_tag="IVK93_002419"
     CDS             complement(4340..5440)
                     /locus_tag="IVK93_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6387666.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            5618..6403
                     /locus_tag="IVK93_002420"
     CDS             5618..6403
                     /locus_tag="IVK93_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP6387667.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6436..6819
                     /locus_tag="IVK93_002421"
     CDS             6436..6819
                     /locus_tag="IVK93_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP6387668.1"
                     /translation="MSNLRDYNQEAPIHCLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            6895..7026
                     /locus_tag="IVK93_002422"
     CDS             6895..7026
                     /locus_tag="IVK93_002422"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6387669.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            6995..7231
                     /locus_tag="IVK93_002423"
     CDS             6995..7231
                     /locus_tag="IVK93_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6387670.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(7309..8280)
                     /locus_tag="IVK93_002424"
     CDS             complement(7309..8280)
                     /locus_tag="IVK93_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP6387671.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         2578 a         2042 c         1717 g         2501 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtgactgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaattgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaaggaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccaaaat caattctacc
      781 ttagatgttc ttatatcact ctatcagtcc tatttgacaa agagccagaa atgtttgccg
      841 actatacact agctttgttg tgcaaaatgc tgagtatcaa tttccacagg gattgctaga
      901 atatctttca gctgaatgta taaggcatcg ctaattttct cttcccgatc gttcaaccga
      961 tccactaaaa tttctttgcc ctccagcgtt tgaaaaacat accgttgaat gttgcctaaa
     1021 ataaatgatt ccttccatag aaccggaatc ttaaccccct gaggaaccgg ttcaaattga
     1081 atcagctccg gtcttaaata ataaatgcag cgattgccct tagcggaaaa ctcgtgggta
     1141 tactgattca gctcatacca ttcgaaacga ttatagttgc cgataaattc cgcaacaaat
     1201 cggctctgag gattctgata caattccttc gaggtggaaa cctgagccac ccgcccagct
     1261 tccatcacaa aaatccgatc actgattcgc atcgcctctt cttggtcatg agtcacaaaa
     1321 atcatcgtca tccctagcga ttgctggata gcgcgcaaat cccgctgcag ctgttttcta
     1381 attcgggcat ccaaagcact taatggctca tccagcagca ggactttcgg ttcagtgacc
     1441 aatgagcgag ccagtgccac ccgttgtcgt tgaccgccag aaagaaactg gacattttta
     1501 tctgcctgct ccgtcatatc caccaatgat agcatctcaa aaaccttttc ctgaatcacc
     1561 tcaggactca gcttctttac cttcaaacca aaggctacat tttcaaaaac agtcatagtt
     1621 ggaaacaatg catactgttg aaaaacaaag ccgatattgc gatccttcgt aggcttatcg
     1681 gcaatattct cattgtccag cacaatctca ccgctctttg gttgttccaa tcccgtgata
     1741 cagcgcagca acgtagactt tccgcacccg ctgggaccta gtaaagtaac cagctcaccc
     1801 ttttcaatct gaatttcatc aatagctaaa atctgttttt catcaaaaaa cagattgaga
     1861 tcatgtattt ctaaaaacat tttccgtcac aactccttta agggcttctt tttcgactgc
     1921 ttctcttccc tcgtctgctt aagggacagc tgttgctttt gtcgataagt cagcataaag
     1981 gtagttaaag caattcccac cataaacagc aaatacaaaa ccatcacagc gctagccaaa
     2041 tggccgtttt cgttcatccg ccgcagcata tagatgcgta ccgtctgaaa tcgaccgcca
     2101 atcaacaggt tcgtcagcat gtactcccca aaaaccgaag aaaaaatcat cagagaaact
     2161 agggtcacac cgatacggat gtttggaaac agcacctgga taataatcgt agacatcggg
     2221 gcgcccaagg tttctgctgc atccactaat tccttcagat taattaagcg catctggttg
     2281 ttaagactaa gatagacaat cggcaacgcc gtaataaaca atgcacctac caacactaca
     2341 aacattggga taccagtttt agcatacgtt cgaagcaaag ccgttaccaa aatcactcct
     2401 ggtaaagcat acggcagcag gagcagcttt tccagccaac gattcaaacg agggaaatac
     2461 aaatgaatcc aaataatggt gggaatcatc aacaacagaa tcgtcaccaa gaccacacct
     2521 gctaataaaa gtgagcgacc caccgcagca ataaattccc tgtccgtcag cagctgttgg
     2581 aaccattgca aggtaaaatc actaggtaaa agcgattttg accatttagt ggagctggca
     2641 tacaaaatcg ttccaataat cggcagcaag taatacagga caaagcagaa caaaatcggt
     2701 tttattaact ttttcacgtc tgcaccacct tgcctttctt ttgaataatc aacattgccg
     2761 ttaccagcag gacacctaaa gcgactgcca acgcactgcc taaattaggc ttggcgaaca
     2821 catctcccga cactaaagcc gcaattcgaa tcgttaaaag atttacatta ctgctggtca
     2881 gggcatacgc tgtttcatag gtccccatgc cattagcaaa taagatgaca aaggtgctgg
     2941 caatcgatgg tcgcaaaaaa ggcaaagcca ccttgaacca agcatacgct gatgaagcgc
     3001 ccagcaattg tgcggactcc agccattcct ttttcacttc cttcatgctg ggatacaaaa
     3061 acaagacccc taacggaatt tggaaatagg tatacgtgat ggtcaatccc caccaagaat
     3121 acaaattgaa gtccgctaga aatggcaaaa tcattttcag cacacctaca ttccccaaca
     3181 aaataataaa agagaatgcc agcggaatcc cagcaaaatt agctgccaaa ttggaaacaa
     3241 aggtgatttt ctcctgcaat ttagtcgata gattcattaa gcataaacat aaaataaacg
     3301 tcccgattaa cccaatcaca ctggagctga cagcaatcag tacgctgtta acaaaggcct
     3361 gtgtgtaata tttgttgctt aaaatatcac tgtaattggc caatgtccac tggttagtta
     3421 aatcactgtg caagctgcac agaaccataa aaagcagcgg cagcagtaaa aatgcacaga
     3481 agataaagga ggcaggaacc agaaccaagc gttttttcat ctctttcatc ccctcatcaa
     3541 ttcttgaatg tcctgcattt tctctgacgg tgcaattcct aataaccagc aaaacaatgg
     3601 agccgccagc agctgtggta cagtttgttc cttcagcttt tggcgtttat ttgaaatcaa
     3661 ataaaaagga acttcacgat gtgcagccaa gctgccgcca tgcagtccgt gcgcatccat
     3721 cccgtgatcc gcagtcacca cgacttgata acctttttgc agccattcag gaaggtaaat
     3781 agccaataat ctgtccgcct gattaaccgc tgctgcatat tcatgagaat cggcggtaaa
     3841 tttgtgaccg acatcatcaa tattcatcga atgtacgacc aaaaagtctg gctgatactg
     3901 attcactaaa taatgggcat ccgcaaataa atgcgaatca gggtaagtgt cctcactata
     3961 aaagatgcca tgctgaatca gtcgctcctc ctgatgctgg atgcgatcaa ccaaacgatt
     4021 atagggggct tgattgtaca actcgctgta ccagtggtaa gcggcggctc ctgtttttcc
     4081 gccagcttcc ttaactaatt caaacagcga ccactctttg gatcgctgag catggtaatt
     4141 actatagatg ccattttcat aggctggaac gccggtcatc agtacttcgt ataacggacg
     4201 tgaattgctg ggcagctcac tttttacctt caataagctt gcttgctggt gctcaactaa
     4261 atgattcatg aacccaagct gggcgacggc tgtgtcgtag cgacagccgt ctagtataac
     4321 tacaatcagt ttgtcaagtc tactttgcat aggtcagtac ctccgcctgc caaagctctg
     4381 gaagctctac agaggtcttg ttccaagctt cattgtcttc aactggcacc gccgcttcat
     4441 aagcatcctc cggcagtaat ttttcagcta cttctttagg cagctctacc ttgcgaatag
     4501 gtcgcgcata gccctttgcc aaattgattt gcccttcatc agataaaata tattcccgtg
     4561 ctagctttgc cgcattggga tgaggggcat ttttattgat taaggttgta tacccgctaa
     4621 tgatggagcc gtctgttggc acagttacct caaaacgctc ggagtcaatt tggtcacgat
     4681 aatttagtcc attgaagtcc caaacaactg ccacatcgat ttcacctttt tcaagatttg
     4741 ctacgctggc atcaacggtg gacaaacggc ctttttctgc catttttttg tagtaattca
     4801 gacctggctg aatatccttt tcgtcacctc cgttggcaat cgccgcagcc aatacagcaa
     4861 attgtgcttg gttggcaatc gtcacatccc ccacgctaac ttgatagtca ccctcttgca
     4921 gctccttcca gctagtcggt gcttgcttca cattctcttt atcggtgata aaggaaatgg
     4981 ttcccgtata gcttaacaac caatccccat catcatcctt tgcccaatcc ggaatttcct
     5041 cccaatagtt ggtcttataa ggtaaggtta actctttgct ttcagccaac ggaccgaagc
     5101 tgataccgac atcaccaata tcggcggttc cattttttcc ttctgattca aacttagcca
     5161 attcttctgc actggacata tccgtatcca cgtgacttaa gccgtattct ttttcaaggt
     5221 cgttccaagt gcctacccaa tttgcccaag tatccggcat tcccaagctg gcaatctctc
     5281 cttcttcctt ggctttttct ataatcgtat ctaaagagtc gtctgccgca gccgattgct
     5341 tcgttgcttc acctgagccg cagcctgtca gcaggatact tactgttgct gcacaaaata
     5401 aaccgatagt catgttacga attttttcat tcattttcat tcattcttct cccctttcga
     5461 taattccatt ctaacaaagc tttgtaatga gagaatcaat ttcttgaaaa ggtttcatga
     5521 acaatgtaaa ggttgtatat ggtttgtaaa tttcgacgaa ttcccgttaa ccttctaaag
     5581 cttttgcggt atgatagtag aaaaaaggag gatccctatg cagtattatg atcagcatct
     5641 ccacacgtat ttttcctttg attcagagga aaaatttgaa aactatttag cctatcagcc
     5701 ggagtttttc gtttctacgg atcactttga tttaaacaat ccatctaccg gaaaagatga
     5761 cattccggat tatccagcct atataaacaa actggactct ttaaaagaaa cctattccac
     5821 caaatttctg cgaggtatcg agattggcgt tgttccagga caagaagagc ggatcgtgga
     5881 ttacctcgct cagcatcctt atgatttaaa gctggtaagt attcatcaaa atggccgctt
     5941 tgattatatg gacgatgtgg ttttaaccaa agatcccatt caaactacca aggaatattt
     6001 tgatcagatg ttgcaggtct tgacggattt tccagaagga gatattttga ctcactttga
     6061 ttatggatta cgccgcttca ccttcacagc ggaagaattc gcggagcatt ttgaggctca
     6121 gctgctaacg attttcaaaa aggttgttga acgagaattg gcgatggagt taaatgccaa
     6181 gagcttcttg aaatacggca acgaagacct ttaccgttat gccgttcctt tgtatcaaag
     6241 tgtcggcgga aagctcttta ccttaggctc ggatgctcat gttgccgaag attatcagct
     6301 gggctttaaa aaaatgagtc agctgttggc agactgtcag gtagaaaagt tgctagtcat
     6361 tcaaggaaaa gaacgccgcc tgacgccgct gccgattttg tgattcctat ataatagaag
     6421 aaagtggtga aaaaaatgag caacctgcgg gactacaatc aagaggcacc catccattgt
     6481 ttaattgcgc gacattggga tgcgttgaaa attgaggcgg tttgccgatc cttgttagca
     6541 gccgttccaa aacaacaact ggaaaatttt ttggtggctg acagcttgca gcgggaaaaa
     6601 gttcaggctt attttgcagc ctttaaagat cagcctttgg aatatttgca tgcgcagttt
     6661 catctttttt atcaagtagc cgccccagat gactacaacg atttacgagg acagctgcaa
     6721 ttgacctttc aagcggatga aaccgcctat accgttttat tgggaatggc tcgccttggt
     6781 gatcaggcga aggtcgagtg gcggattttc gatatttgat ggcaggatca aggcgtttca
     6841 gttgcaaacc ttcctctctt tcaattagaa aaaccgcagc aggaagaagt ttgtgtgctg
     6901 gtcaccgatg ctggtgaaat tcgtctccgc ttatttcctg accaagctcc cctggctgta
     6961 gccaactggc gctccctagc tgagcaaggc gtttatgaca acacaccttt cggctcgtgt
     7021 cattaaggaa tttgttattc aaggtggcgc tttggatggt tctggtgatg aagctgtttc
     7081 cagctatgag ggctttttcg ctgatgaagt gcatcgcgga ctttatcatt ttaatggcgc
     7141 cttggcctta ggggaccacg gcccccacac caacggcaac cagtttttta ttgtgcaaaa
     7201 cacaaaggcg caggctgacc tgctaatgta aagcaagcat acttggagct tggaggacta
     7261 ccggagctgg atggtcgtta tactgttttt ggataagtat accaagggtt agaaatcgta
     7321 gcagcgatcg cagctcagcc gacaaacagt gaagatgtac cgattgagcc agtgttcatt
     7381 cgacaaatta acttcgaaaa aacatcgtaa aaagacactc acagacttca gcaaaagctc
     7441 tattcttgag ctggctgcag ctagtgagtg tctttcattt atccataaga taaagcttgg
     7501 attcaataaa taaataaatg ttttggttaa agctgtgacc agcaaatgag gaaccgtgcg
     7561 agccagttga tctgccaatg caaacatttc tttaggtgaa ggcgctggcg tgtctgccaa
     7621 cggatactcc agtcccaatg cttggtaacc gtgcatacca tagcagtggt acggtagtaa
     7681 ttctaacggt acgaaccgat caagatctgc cacaaaacaa gcaatcgtca gcagctcggt
     7741 caggttcata ttgatacctg gaaccacagg aactcgaatt cgcacttcgc agttaggata
     7801 ctcaattaaa aagcgacgaa tattcttcaa aatttgttgg ttatctaagc cggtccattg
     7861 tttgtggagc ttgctggtaa acatttttac atccacaaac aatgtatcca catagggagc
     7921 taccttttgg atttcattat aagcaccaca aaagctggtt tccagtacgg tattgattcc
     7981 aatgtatttc gatttttgca aaatttcttt agcaaaatcc gcctgaacca gtgcctcgcc
     8041 tccgctaatg gtcactccgc catccgagtg aaagtaaaaa acagcatcct tctcaatctc
     8101 atccattacc tcttcagcgg tcatggtctg gccaaatcct ttttcaatct gcttgctttg
     8161 tgattcggga atcgaacacc aggcacagcg taatgggcag ccctttaaat aaacaacggt
     8221 ccgtaaacca gcaccatcat gtagagaacc ctgttctatt cttaatactg ttccttccat
     8281 atagattttt gaattacaca ctagtttaaa ctaaaacaca caaggaaaaa cttaccattt
     8341 aatcataaaa ttagctcata taaatatact aggatgtaaa atagactggt caaaatctgg
     8401 tcaaaaaaga ggactaatac aggtagtcct cttttttgtt attttaatat tctaaatcat
     8461 tttttattgg tttattccct tattcgatgt agactcaaat cactatatca ctagatatca
     8521 aacggcgtca tacggaatca aaaacatcaa atttgacctg ttaaatggtc attgaataac
     8581 attcaaaatc aataaaacgg agtcagacac ggagtcaaaa acgctgagag taaaaaatat
     8641 atacagcctt gtaggtaaag taccacaggg ctgtatattt tttatttcag attaccttaa
     8701 atgattccat ttacttttta tctcattttt tcctactagt tagtatcaaa tactctccta
     8761 ttttctcaat aaactatttt tggatacatg gtatctgaaa cagctaaaac cttgtcatta
     8821 cagcaaaaaa tcacgtta
//
LOCUS       DACCCR010000098         8653 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCCR010000098 DACCCR010000000
VERSION     DACCCR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.105"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(175..732)
                     /gene="frr"
                     /locus_tag="IVK93_002425"
     CDS             complement(175..732)
                     /gene="frr"
                     /locus_tag="IVK93_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP6387672.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(735..1457)
                     /locus_tag="IVK93_002426"
     CDS             complement(735..1457)
                     /locus_tag="IVK93_002426"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP6387673.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1593..2474)
                     /locus_tag="IVK93_002427"
     CDS             complement(1593..2474)
                     /locus_tag="IVK93_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP6387674.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2573..3355)
                     /gene="rpsB"
                     /locus_tag="IVK93_002428"
     CDS             complement(2573..3355)
                     /gene="rpsB"
                     /locus_tag="IVK93_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP6387675.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3714..4193
                     /locus_tag="IVK93_002429"
     CDS             3714..4193
                     /locus_tag="IVK93_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP6387676.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4410..5501
                     /gene="phnW"
                     /locus_tag="IVK93_002430"
     CDS             4410..5501
                     /gene="phnW"
                     /locus_tag="IVK93_002430"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP6387677.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5494..5622
                     /locus_tag="IVK93_002431"
     CDS             5494..5622
                     /locus_tag="IVK93_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP6387678.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5626..6171
                     /locus_tag="IVK93_002432"
     CDS             5626..6171
                     /locus_tag="IVK93_002432"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP6387679.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6625..8316)
                     /locus_tag="IVK93_002433"
     CDS             complement(6625..8316)
                     /locus_tag="IVK93_002433"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP6387680.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         2404 a         1771 c         1568 g         2910 t
ORIGIN      
        1 aaagattccc actatcttaa gtattatagc aatatctaac tgtttttcct atgaaatcat
       61 tgttttttta tttaaaaact gaataatttt ttcttttttt aatttttaaa taaaagaaat
      121 ataaataaaa acagaggaag cggaatgatc cgcccctctg tttttatgat tttattaaac
      181 ttccaataat tctttttctt tatcagcagc gatcttatct acttctttga tgctgttatc
      241 tgtcaatgtt tgtacgtctt tttctaatcc gcgcaaatca tcttcggtaa tatcaccatt
      301 tttttgttgt tttttatatt catcgatcgc atcacgacgg atgttacgaa tcgcgatttt
      361 tgcattttct gcttcttttt tcacatcttt agctaattct tttctgcgtt cttctgtcag
      421 ttgcgggata actaagcgaa tgacatttcc atcattggtt ggactgattc cgatatcact
      481 tgccataata gctttttcaa catcttgaag aatgctcttg tcaaaaggtg tgatcattaa
      541 aacacgtgct tctggaattt ggattgatgc catttggttc aatggtgttg gcgcaccgta
      601 gtagttcaca gtaatacggt ccaaaaggct agcgttcgca cggcctgcgc ggatttgccc
      661 aagttcacgt tgaagatttt gtgctgcttt tttcattttt tcttttgctt ctgtcataat
      721 tgcatctgcc atttttattt cccccttaca gttgttccga tattttcgcc taagattgca
      781 cggcgaatat ttccgtgttc atttaaattg aagacaagca gtggaatatc attatccatg
      841 cttagtgaac ttgcagttga atccatgact tgtaatcctt tggcaataac ttccagatga
      901 gtcagttctt caaattttac tgcatttgca tctactttcg gatcagcaga ataaacaccg
      961 tctacattgt ttttagccat caggataaca tctgcgccga tttctgccgc gcgtaatgct
     1021 gctgttgtgt ctgtcgagaa gtacgggttg ccggtaccgc ctgcaaagat aacgatccgt
     1081 cctttttcta aatgacgttc tgctttacgg cgaatatatg gctctgcgat ttgacgcatt
     1141 tcaatcgaag tttgcacgcg tgtcggtaca cccacgtttt ccaacgtatc ctgtaaagct
     1201 aaggcattca taactgtagc taacataccc atgtaatcag cttgagcacg ttccatgccc
     1261 atttgtgctc cgatttgtcc gcgccagata tttccgccgc ccacaacgat tgccatttca
     1321 acgcccagct cgtgaacttc tttgatttct tggatgattt ctttgattac tggtggtttg
     1381 attccaaatc ctgcgtcccc agctaaagct tctccactca attttaagac tacacgctga
     1441 tatttcggtg tgaccatgtt cattcctcca atattttcta ttgccatttt accatactta
     1501 gatgatttcg acactctaag actggttttt cttgttttgc ttaaaaaaag ggagcgcaca
     1561 aaccgtgcgt tcccgtctgt tattcaacag aattattttt tcacttggct cataacttct
     1621 tctacaaagt tatcttcgcg tttttcgata ccttcgccaa cttcgaaacg tacgaatgtt
     1681 ttaacagttg cgccttttga tgcaacgtat ttttcaacag tcatatcagg atctttaacg
     1741 aatggttggt ccaccaaagc gatttctgct ttgaatttct tcaagcgtcc ttctaccatt
     1801 ttttcaacga tattcgctgg tttgccttcg ttcaatgctt gttctgtaag aacagttttt
     1861 tcatgttcta attcagcttc tggaatttgt gattcgttta cataacgagg attgatagct
     1921 gctacgtgca tagctacgtc tcttgcaact gtttcgtcag ttgttccatc tagaacagtc
     1981 aatacggcaa tacgtccgcc catatgcaag tatccaccaa atgcagcatt gtcgtctttt
     2041 tctacaactt caaagcgacg gaagctgatt ttttctccga taacttgagt tgcttcgatc
     2101 aagtcagatt caattgtacc tttatctgtt ttgattttca ttgcagcttc catgtctgct
     2161 ggtttgtttt cagcaacaag ttctgcaatt tcttttacta aatcttggaa catttcgttt
     2221 ttagaaacaa agtctgtttc tgagtttact tcaacgattg ctgcagtgtt acctttcaca
     2281 gcaactgaag ccaatccttc agctgcaatg cggtcatttt tcttcgcagc ctttgccatt
     2341 cctttttcac gtaaaagatc aactgctttt tccatgtcgc cttctacttc aactaatgct
     2401 tttttcgcat ccatcattcc tacgccagtc atttcgcgta gttcttttac cattttagct
     2461 gtaacgtctg ccatgtttaa ctcctcctgt gtgtttttct ttttaaaaaa agctgcttca
     2521 aaggagagtg aggcttgtcg cccagccttt gaaacagctc catcattcaa aattattctg
     2581 ctgaagaatt gtcgccttca acaacatcta cgatttcttc gattgatgtt gctgtgtttt
     2641 cttctgtgaa atcttcttca actacttgat cttcaccttg gtttccttcg atgaaagcat
     2701 cagccatttt tgaagtaatc aatttaacgg cacgaattgc atcgtcgttt gaagggatta
     2761 ctacgtcgat ttcatctgga tcgcagtttg tatcaaccat agctacgatt gggatattta
     2821 atttgtgtgc ttcttgaaca gcaatacgtt ctttacgtgg gtcaacgatg tacattacat
     2881 ctgggattct tggcatatca gcgataccgc ctaagaattt ttcaagacgt tcacgttctt
     2941 tgttcaaacc agcaacttct tttttaggta gaacttcaaa agttccatct tcttccattg
     3001 cattgatttg tttcaaacgg ctgatacgtt tttggatagt atcccagttt gtcaatgttc
     3061 cgcctaacca acgatggtta acgaagtatt gtcctgcacg agttgcttct tctttgattg
     3121 cttcttgtgc ttgttttttt gtacctacga ataatgctac gccgccttct tcagcaacgc
     3181 tcttcatgta atcataagct gcatctacta atttaactgt tttttgcaag tcaatgatgt
     3241 agattccgtt tctttctgtg aagatatatt tcttcatttt tggattccag cgacgagttt
     3301 ggtgaccaaa gtgtacgccg gcttctagta attgtttcat agaaattact gccatgtttg
     3361 tttcctccga tttggtttta tttttcctct tcttatcttc taccttgccg gcaaactttt
     3421 tcaagcacct accgacaatc caataagaat gtggatttgc tgatgcataa agcaccctca
     3481 gctattatac agacaaaaga tccgtatcgc aagagcaaat atacttttct gctcaacatt
     3541 caatagttta cctgaaagat aaaaagaatt caagaaaaac agtagatttc tctttgtttc
     3601 tttattatcc tttttccata aaataatttt aggtataaaa gacgaatatt attgggcaat
     3661 ccttcttttt tttgttatga tacatgtgaa atctttcgta aaggagaaag aaaatgcgca
     3721 aagctcaaag acatttgctg attaaacaag tcattgaaga attcccgatc cgtacgcaag
     3781 aagaactatt aaacaaactt gggcattatg gcgttaccgc cacgcaggca acgatttctc
     3841 gagatatccg tgatctaaaa atcgtgaagg cacctgatga aaatggcgtt tcccgcttcg
     3901 tattgtttcg cgaaaaagga actcctgaga ctaggactga ggaagaggat cggctggttc
     3961 aaatgattga agacattgta ttgaaagtcg aacgagtcca ctttttgacg atcgtcaata
     4021 ccttgccaga caatgctcat ttatttgcat ctgttttaga tgagatcaaa cctccctcta
     4081 ttgtcagtac aattgctgga ttcgatacga tcatggtcat ctcggaaaat gaggaggacg
     4141 caaaacgagt agaagaattt cttcaccatc caactgaagt atcacttttt taatcagagc
     4201 acgtattcct aatatggaat gcgtgttttt ttattttcaa tattttcctt attgaataaa
     4261 cctttacact ctttacaaaa atacatcatt ttttattcaa atttttcaac accttgattg
     4321 aatcacaata atcatcattt atattggatg ttaacaagag cagcttcttg ctctttgtgt
     4381 atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt attaacttct ggaccattga
     4441 cgacttctga aacagtgaaa aatgctatgc tgatcgatca ttgtacttgg gatcaagaat
     4501 acaaagaaat tacacaatgg ataagaaaaa aattattgga tcttgctcaa gtatcggaag
     4561 gaatatacac aacggtctta atgcaaggta gcagctcctt cggggtagaa tcggtattaa
     4621 gttctgtgat caggccagaa gatactgtac tgatttgttc aaacggcgca tatagacaac
     4681 gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac cgtttatgag gtatcggaac
     4741 atcaaatcct ccaagctcat gtgattacac agctgattga agagaatccg tctatctccg
     4801 cactagcaat ggtccatagc gagactactt ccggtatttt gaataatttg gaagagattg
     4861 ctgctgtcac acaagaaaga gatcttttgt ttatagtgga cgcgatgtca agctttggtg
     4921 ggataccgat ttgtgtagcc gaactaggga ttgatttttt gatttccagc tcaaacaaat
     4981 gtattcaagg tgttccaggc ttttcttttg tacttgctaa taaagcggaa ctggaaaaac
     5041 gaaaaaatgt tagccgaagt ttgtcacttg atctatatga ccagtaccgc gtgatggaaa
     5101 acgagaatgg caaatggcgt ttcacttccc ctacccatac ggttttggct tttcaacaag
     5161 cgctgaagga actagaggca gaaggaggaa tcgaaactcg atattcacgt tatctcgcca
     5221 ataatcatgt gctacgacaa aaaatgacag agatggggtt ccagcctttc attcaagaat
     5281 atcaggggcc attcatcacc tcttttcttt accctaagga ctggcgattt gattttaatc
     5341 atttttatca gtatttaaag caaaatggat ttgtgatcta tcccgggaaa atcagcgatc
     5401 atgaatgttt tcgtatcgga aatattggcg agatctatcc ggaagatatt ttggaactga
     5461 cagcaattat caaagactat aaggagaacg ttaatgatta aaactgtaat ttttgattgg
     5521 gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc acgcttttag aaatgctttt
     5581 ctagaaaaag gaacccaact gactgacaaa gaaatccgct aaccgatggg aaaattaaaa
     5641 tgggatcata tccaacagct tctttccctc ccttctgtca aaaaacaatg gatagctatt
     5701 tatggacaac tacctcagga aaaagaggtg gaggaattgt atcaacaatt tgaacattat
     5761 ctgtttaaag atttggtcaa gcattctacc ttgaaacccg acacattgga aacgatcgaa
     5821 cggttgagaa acgctgggat caatattggt tccacgaatg gatttaatgc tcctatgatg
     5881 acaatcgtag cagaaaccgc aaaaaaagaa aggtattcac cagacttcat ggcaactttt
     5941 gaagacgtcg aagggtatgg tcgaccgttt ccttatatga tccataaaaa catgcagtac
     6001 ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag atacggtctc agatatccag
     6061 gaagggaaaa atgccggagt gttgacagta ggtgtaattg aaggtagttc gctaatgggg
     6121 atttcttatg atgagtacca aaaattagag atggggaaaa agatcgttta aaacagtctg
     6181 ttgctcttag attccaacaa gccggcgctg atatttgtgt aaacaattta aaagagttaa
     6241 cagaatacat tttgtacgct gacaagaatc cagaatgact tttatacaaa ataagaggtt
     6301 gtgaaaaagc tgtcctgtat caagtaataa aaacagccaa gcaaaaatag ctcctcatat
     6361 ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
     6421 aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc ttatttgcaa acaaacagtg
     6481 tttaagagac aacctctcca ctaaacggtg ttcattcagc tgactctcgg tatttgctca
     6541 aaaaacgata aaatttgaaa agctattttc tcttttgttt ctctaatact cggatcagaa
     6601 cgctgatttc acattctctt tttcttacat cttatctggt gcatgaagtc ctagcaaacg
     6661 aaggtcttct ttcaataata cagtcactgc ataaactaga gctaagcgtg cttctttttg
     6721 ttcatcatca gctaaaatct tcgtatgagc atagtatttg ttgaatgctt gtgctaattt
     6781 gattgcatgt ttcgcaatca ctgacggttc gtatttttcc ccagcagaca agaccgtttc
     6841 agggtatttt tgaactaatt taaccacttc ccagctggta tcgtcattca atgcatagtc
     6901 tgctgtttct gaagggacaa atcctgcttt ttccaacagg ctgactgcac gagcatgtgt
     6961 atattgcaca taaggtccag tttctccttc aaatcgaaca acttcttcca ggttaaagtc
     7021 aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg actgccccaa ccccaacttg
     7081 tttagctaca gcatctttgt tttccaaatc agggtttttc tcgctgattt gttctttagc
     7141 agattggatt gcttcattta atacttcttc taataagacg atctttcctt tacgagttga
     7201 taatttttta ccaccttgcg tgatcaaacc aaatggaata tggtgcatgt cgtcagacca
     7261 gtcaaagccc atttccttca agacagcttt caactgttta aaatgatagc tttgttcatt
     7321 ccctactaca tatagagatt gtttgaagtc gtaagtacgt ttacggtaca atgcagcagc
     7381 taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc ttaatcaacg ctggattcag
     7441 gtcataagca gataggtcaa cgatctctgc acctttatcc tcgttcagca aatgtttttc
     7501 ttctaaaagt ttgacgattt catccatctt gtcattgtag aaagcttcac cgttcaacga
     7561 atcgaaacgt acttccagca gatcatagat tttattgaat tctttcatcg attcatcacg
     7621 gaaccattgc cataattgaa tagcctcttt atcgccttct tccagacgtt tgaaccaagc
     7681 acgagcttct tcatttaatt ctggttctgt ttcagctact tcatggaatt ggacgtataa
     7741 acgaagcaat tcattgattg gttctgcttt aacagcttct tctgtacccc attttttgta
     7801 agcgacgatc agttttccaa actgtgttcc ccagtcacct aaatggttga tgcggattgg
     7861 ctgataaccg attttttcca taatgaaacc gatcgaatta ccgataactg tcgaacgcaa
     7921 atgtcccatt gagattggtt tggcaatatt aggagaagac atgtcgattg gtactgttcc
     7981 ttgattcccg atgttgctat ccccataatg ttctttttct ttcactactg tttgcaaaac
     8041 ttttttgctg ataagttctt tattcatgaa aaagttcaag taaggtccga caacttcgat
     8101 tttttcaaag ttggcagaat cgattttttc tgctaaatct gcagcgattt gctgtggcgc
     8161 ttttcggtaa acttttgcta atgaaaaggc tggaaatgca acatctccgt gttcagctga
     8221 ttttggattt tctagtaatt gctcgacttg ttccagtgtt aaatcatctt tgacagcgtc
     8281 atagacagct tttgcgacaa gatctttatt attcatcttt attcctccta aagtgttccc
     8341 ttgattgtta aacaaaaaaa atctctagcg acggcatact tagtatgcca tcactagaga
     8401 cgagatttcc cgcggtacca ctccaattgt cctaaaaaag gaccagctcc atctgatatc
     8461 ggtagacgac cgtctgttta aaacagaatt ctccaagtgc gcttcgttgt atcttcttca
     8521 ttcggcttcc accacttccg aattcgctag ccaagaatga tacaagtact ctcttgttca
     8581 acaaatctta gtcttattaa actttcttga attatatcag aaaaaaaaac gaaaggctag
     8641 tcgtttcata aaa
//
LOCUS       DACCCR010000099         8608 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCCR010000099 DACCCR010000000
VERSION     DACCCR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.106"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            211..1080
                     /locus_tag="IVK93_002434"
     CDS             211..1080
                     /locus_tag="IVK93_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP6387681.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1289..2314
                     /locus_tag="IVK93_002435"
     CDS             1289..2314
                     /locus_tag="IVK93_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6387682.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2353..3303
                     /gene="manA"
                     /locus_tag="IVK93_002436"
     CDS             2353..3303
                     /gene="manA"
                     /locus_tag="IVK93_002436"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP6387683.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3397..5100
                     /locus_tag="IVK93_002437"
     CDS             3397..5100
                     /locus_tag="IVK93_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6387684.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5102..7135
                     /locus_tag="IVK93_002438"
     CDS             5102..7135
                     /locus_tag="IVK93_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6387685.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7146..7586
                     /locus_tag="IVK93_002439"
     CDS             7146..7586
                     /locus_tag="IVK93_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6387686.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7786..>8608)
                     /locus_tag="IVK93_002440"
     CDS             complement(7786..>8608)
                     /locus_tag="IVK93_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387687.1"
                     /translation="EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDL
                     PAHLPLANYYENIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKH
                     SIRFTIYYGAAMLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQ
                     SFLFLLLWVLFLSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYL
                     PTSYVDFQNVIIGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
BASE COUNT         2947 a         1479 c         1781 g         2401 t
ORIGIN      
        1 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
       61 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      121 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      181 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      241 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      301 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      361 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      421 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      481 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      541 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      601 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      661 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      721 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      781 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      841 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      901 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
      961 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1021 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1081 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1141 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1201 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1261 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1321 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1381 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1441 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1501 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1561 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1621 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1681 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1741 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1801 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1861 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1921 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     1981 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2041 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2101 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2161 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2221 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2281 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2341 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2401 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2461 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2521 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2581 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2641 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2701 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2761 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2821 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2881 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     2941 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3001 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3061 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3121 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3181 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3241 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgactga accaggcaaa
     3301 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3361 atccgtccat caataaatag aagtatggtg aaaaagatgg aatacaaatg gaaagcaaac
     3421 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3481 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3541 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3601 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3661 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3721 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccac
     3781 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3841 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3901 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     3961 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4021 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4081 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4141 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4201 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4261 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4321 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4381 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4441 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4501 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4561 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4621 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4681 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4741 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4801 gatatcacag aacaaaaaaa gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4861 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4921 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     4981 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5041 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5101 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5161 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5221 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5281 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5341 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5401 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5461 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5521 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5581 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5641 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5701 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5761 ggttttgatg ctgacattga cgatggagcg tgtgactaaa aatcatttac tgacaaatga
     5821 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5881 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     5941 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6001 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6061 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6121 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6181 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6241 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6301 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6361 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6421 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6481 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6541 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6601 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6661 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6721 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6781 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6841 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6901 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     6961 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7021 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7081 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7141 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7201 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7261 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7321 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7381 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7441 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7501 gttcagacat cgaccaagta atggcgctca gtgatgcgaa aaccattgaa gaagtccaaa
     7561 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7621 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7681 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7741 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7801 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7861 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7921 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     7981 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8041 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8101 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gaaggattcg
     8161 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8221 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8281 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8341 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8401 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8461 agggagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8521 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8581 aatcagtcga tccagtatat ctttttca
//
LOCUS       DACCCR010000100         8490 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCCR010000100 DACCCR010000000
VERSION     DACCCR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.107"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..173)
                     /locus_tag="IVK93_002441"
     CDS             complement(<1..173)
                     /locus_tag="IVK93_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6387688.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IVK93_002442"
     CDS             complement(593..1714)
                     /locus_tag="IVK93_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP6387689.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IVK93_002443"
     CDS             2042..3451
                     /locus_tag="IVK93_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP6387690.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IVK93_002444"
     CDS             3448..4176
                     /locus_tag="IVK93_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6387691.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IVK93_002445"
     CDS             complement(4304..5215)
                     /locus_tag="IVK93_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6387692.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IVK93_002446"
     CDS             complement(5232..6239)
                     /locus_tag="IVK93_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP6387693.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IVK93_002447"
     CDS             complement(6236..8365)
                     /locus_tag="IVK93_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6387694.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IVK93_002448"
     CDS             complement(8370..8477)
                     /locus_tag="IVK93_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387695.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DACCCR010000101         8369 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCCR010000101 DACCCR010000000
VERSION     DACCCR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.108"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..738
                     /locus_tag="IVK93_002449"
     CDS             <1..738
                     /locus_tag="IVK93_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP6387696.1"
                     /translation="LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDE
                     VDTITNNNLQMASVSKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNK
                     NWNPKQPILTTIRQRYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANR
                     VNELASIHEIIWYQTYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEE
                     RLPFCQYQVPLEISGFNYRQSSVSGYN"
     gene            866..3847
                     /locus_tag="IVK93_002450"
     CDS             866..3847
                     /locus_tag="IVK93_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP6387697.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            3844..5505
                     /locus_tag="IVK93_002451"
     CDS             3844..5505
                     /locus_tag="IVK93_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP6387698.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            5545..6477
                     /locus_tag="IVK93_002452"
     CDS             5545..6477
                     /locus_tag="IVK93_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP6387699.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(6837..8330)
                     /gene="guaA"
                     /locus_tag="IVK93_002453"
     CDS             complement(6837..8330)
                     /gene="guaA"
                     /locus_tag="IVK93_002453"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6387700.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         2689 a         1477 c         1713 g         2490 t
ORIGIN      
        1 ttaattgggg cattaaaatt agcagattat gtctattctg aagaagagtg gaatgatttt
       61 gaggaaatct acgatcgttt attaggaaca ggtagttatc aattaatgtc cgaattacga
      121 tatttcgatg aagtggatac cattacaaat aacaacttac aaatggcttc cgtgagtaaa
      181 ctgctacttt ggcaagatcc gttagttggt atatatgact ttcatgtagc cgctcataac
      241 aaaattgcca ctagaacaat tggtacttat tatcatgagt tagctcagaa attagatatg
      301 aataaaaatt ggaatccaaa acaaccaatt ttaacaacta ttcgtcaacg atatatactt
      361 ttagctgaaa ttttagcaca aaaggcagaa ctaggcatct atttacaaca ctgctgtctg
      421 gtagaagacc ttgcaggatt aatggaatta gcgaatcggt gtgaagcact agccaatcga
      481 gtaaatgaat tggcatctat tcatgaaata atttggtatc aaacatacaa aacctatggt
      541 tgggaagtat tagaacaacg atatgctgca ttagctcatc gtctgaaaac cgttgcgcgt
      601 cgtattaaaa atcatcttca ggggcaagat gccctagagg agcttttgga agaacgatta
      661 ccgttttgtc aatatcaagt gccgcttgaa atttctggct ttaattatcg gcaaagctcg
      721 gtgtcaggct acaattaacg aaggaatctt cccgttgaac ggaccacttg ctcatctaat
      781 gggaaagtgg ttcgttttct attcataagt agcttaccac taactctttt attaagaaga
      841 gaaaaaaagt aggaagtgag aaaatatgtt atcggactat tatcaaaatc tgtcagttat
      901 aagtgtcaat acagaaccat tacgtaatta ttatattcct tatgcaagta aagaagaagc
      961 aaaacaagag ttagaaaggg aaaaaacgag ccagtttatc gatctcaatg gtacatggga
     1021 ttttcagtat tatccttctg tccaacagtt accggaaaat ttttttcaag aggcctttac
     1081 tgaaagttat gatagaattt ctgttcctgg agtttggcaa acagagggat ttgacttacc
     1141 tcagtatatc aatgtatctt atccaattcc ttttgatccg ccttatgtac ctaatgatac
     1201 cccttgtggt gtatatcggc gtcagtttca aattgagcaa acagatgcac gcttttactt
     1261 aaatctagaa ggagcagata gtaattgttt tgtttggata aatagaaact ttgtaggcta
     1321 tgaccaagtg agtcatagta catctgaatt tgatgtgacc aagtatttac aggttggggt
     1381 taatgaaatt caaattctag tgatgaagtg gtgtgatggt acctactttg aagatcagga
     1441 taagtggcga atgagcggtc tatttcgtcc agtgtatctt cttcaacgac caactatgca
     1501 tctgacaagt tatcaactac ggacgttgac tgatcctaaa aaaggaagcg gaacattaat
     1561 ggtttctttt caacgattta ctcctattga agtatcgtta cctatttctt acgaattatt
     1621 ccatcctgat ggacgtctca tagcgaaagg ggagtgttac tcggaacaac aaataaattt
     1681 ggatgttgaa gattgccttt tttggacagc agaaacgcct aaattatata cattactttt
     1741 aacatgtaat ggtgaatatt ttaaagaaaa agttggcttt cgagcactga cgatcaagaa
     1801 tcgtaagtta ttgctgaacg gaacacctat taaattatat ggagtcaact atcatgacgt
     1861 gaatcagcaa ggcacgccag tgatgacccg agagcagtta cgtgcagatt tgttattaat
     1921 gaaaaaatta aatgtgaata ctgtacgaac ggcacattac cctaaatcac cagattttta
     1981 tgaattggcg gatgagattg gtctgtatgt aatcaatgaa gcagatttgg agtgtcatgg
     2041 tgtggttaat ctatatgata ctgaagcgaa ttatcaacta attgctaatg atccgacatt
     2101 cgaaaagccg attatggacc gagtgatccg ttctattctt ccttatcaaa atcgaagctc
     2161 tatcattatg tggtctatgg ggaatgaatc tggttatgga gtaaattttg aaaaagctca
     2221 agctttagca cggaaattgg atgattcacg gttgattcat aatgaacgct cgattgaacc
     2281 agttattgac caagtgaatg attttagtaa cttggatgtt attagtcaaa tgtatccccc
     2341 actggaagat gttcatcgtc attgtcaaag aagtgatact gaaaaacctt tttttatgtg
     2401 tgaatatagc cacgctatgg gcaacggtcc tggagatctt aaaacgtact tcgatttgat
     2461 tcaacagtat gattcctttc ttggcggttg tgtctgggag tggactgatc atgcaatcaa
     2521 tagaggaact ttagaaacgc catcttattt atacggtggt gattttggtg aaaaatatca
     2581 cgacggcaat ttttgcgtag atggtttatt gaccccagat cggcaattaa cagtaggtgc
     2641 attggagtac cgtaatattc attcgcctat tcaactgatt gctgtagatg aggagctgca
     2701 agaagtaacc ttgagcaata taactgcttt tacagccggt tcagcattta acctttctct
     2761 tgaatggtat atagatggaa aaaaaacagt ttatgaagta atgaatcctt tcatacttga
     2821 gcctggcaaa agttgttcat tgcccatcac agtacctaca acgattcatg gtgctcatac
     2881 ggttgctgtt tcgctatctg tttataaaaa tgatgagctt tatggacagc aacagctctt
     2941 tcttaaaaaa gaaaaaatga gattgccaag aactgctcat cataatgatg caccacaaat
     3001 ttcagtaaaa ggcacagagc tgataatcaa aggagaatcc tttatttata tatatcaatt
     3061 agcaaaagct gcgttcactt ctgtaaagat caatcaaaag gaacttttgg aacgtcctac
     3121 tcaattttca atttggcgtg caccaacgga taatgatcgt aacttagtaa acaaatggaa
     3181 agcagctgga tatgctgaaa tgtctttaga aatcagaaaa aaagactgga aattcgtaga
     3241 tgatcatctc ctgatcgaaa cgtcttatcg aggagttatt ccaggattac agccaatttt
     3301 tacaatgcac atacagttga aaataaatgg tgcaggcgta atcaaccaaa aagtatcagt
     3361 ggagaaaaat ccgatttttc cagatttccc tcgctttggg atggactggc cgttgaatga
     3421 tactttttcg aaggtttctt attgtggcta tgggccgtat gaaagttatc ctgataagca
     3481 ctatgctgcg atgtttggta tttatgagat ggagattgag gaatttgatc agccatatat
     3541 caaaccacaa gaatatggta accgctggca atgtgattgg gtatcaatca aaaatcatca
     3601 aggatctacc atgacggttt atggaaactc taatttttct gctctgcctt atagtcaagc
     3661 tcaattaacc gaaaagtcac accattatga attaataaaa gaaaatagaa cttggttcaa
     3721 tttaagttat aaacaaaatg gtatcggctc acacagttgc ggtccttatt tagaagatcg
     3781 tttccgtttt atggaagata cttttgactg gaatatagtg attactttca aaggagaaaa
     3841 tagatgaaag aaacaaagat caatcaagtc aaaaaaaaat tatctcgtta tcaagaagtt
     3901 gtttatcgaa accattgctt aaaatggtca aaaaataagc tgaatgttcg ttttaatgga
     3961 ttattttatc gtttcgaaca gttagaagaa gtctggaaac cggtaacgaa tttccaatca
     4021 tgtcctgtac agctcataaa tgatccaaaa gaaacatacg atttatgtat aacacacctt
     4081 tataaccagc atttgaaaaa tgacggattt tatttaaagg ttactaatga caatcaactt
     4141 acgattgaat cagcaaacct tcgagggttt caatatgcta tggaagcctt attagagagt
     4201 tttttttcgg taggagatca attattatta ccggaattga ttctgaaaca tgaaccatca
     4261 gtgaaaattc gaggaatcat tgaagggttt tatggtatcc cctggacaca tgaaatgcgc
     4321 ctctctcttt tttcatatat gaaagataac caattgaata cttttatgta tgcccctaaa
     4381 gatgatgaat tattacgtaa aaaatggcgt gaactctacg atgcgcaaga attagacaaa
     4441 tttaaggagc ttttatctgc tgctgaggct agcaatatcg atttttggta cttaattagt
     4501 cctggaaatg atattgatat tacgtgtgaa gaagacattc aagttcttct gaaaaagtta
     4561 gaacaactga ttgagcttgg cgtatttcaa tttggtctat taatggatga tattgactat
     4621 caattaaaag gtgctgctaa acgccgtttt cgagagccag cattcgctca tgcctattta
     4681 gtgaatcgag tagaggaata tcttagcact cggttgtcca atcatgaatt agtcatttgt
     4741 cctacagaat atgataatcg tcatggttct cgctatttag caacgttaag tcaagaaata
     4801 ccagaacaat taccgttctt ttggactggc ccaagcacgt tagcggcgtc aatttctaca
     4861 gaagagctgc aggaaatggc ttctgtctat caacggccga tgctgatttg ggataatatt
     4921 ccggtaaatg attatttaga ggataaagaa ctattgttca tgagtccata tgagaatcga
     4981 acaccgaatc tttctaagga gagataccag gttacaggag tagtttcgaa tccaatggct
     5041 caattagaag cttcaaagtt tacgatcaat agtatggcta attacttatg gaactgtgag
     5101 cgctttgatc ctttggaaac ttggacgtca gtcgttgaaa aagtagttgg aagtaaacta
     5161 caaccagctt atcttacctt aaccaatgct tttccaaatc attacacaag cagtctgctt
     5221 tcagatgaag aattactgct agccaaagac ccagaatggg tcacaaaaga aatggctgct
     5281 ttagtccaag cagtaaaatg gatccagcaa gaacaacata catctagatt gagaacagaa
     5341 ttggagcctt ggttacaagc aatcacagaa agttggactt tctggcaaga atggcaatta
     5401 aaaaaagatc caaaagaagc agaagaatgg ttggctaaga aaaaaacaca ccgcatagga
     5461 cgtgatttag taactgccca tttagagcaa attatcaaaa gttagatatc ttaaaaaata
     5521 aaaagcaaga aaaaggagaa caaaatggtc aagagattaa ttagtgcaaa ctacagtgat
     5581 gtcaagaaaa tgacgtcaaa agagttgaag caatccatca aagcgagtga aggacgggtg
     5641 attgtttgtg aaacggttgc taatactctt ccacaatctg gagaaattac gaatgcagaa
     5701 gttgcgtcag catttggtgc agatttgatt cttttaaatg gtttcgattg ttatcgtcct
     5761 gcagtttttg gactaccaaa tgtgacgatt gagaaggttt ttgatctaca gcaaacgatt
     5821 gaaaacccga ttcctaaatt aaaagaacta gtcggtcgtc ctgtgggaat taatttagaa
     5881 cccattgccc ctaaaacaaa tatgatgagt gagaaagtag aaatcagcca aggaagagca
     5941 agctctgttg aaacgataca aaaagcagaa gaactaggag tcgattttat ttgctttacc
     6001 ggtaatccag gcactggagt aaccaattta gaaattacta aatccgtaaa aatggcaaaa
     6061 aagtattttt cgggattgat tattgctggg aaaatgcata gcgccggctc agaagagcca
     6121 gttgtgaata aagaggcgat tatttcttat gcggaagcag gcgcagatat tttactagtt
     6181 ccttcagtag ggtctatccc aggcttttta gagcaagacc taattgaagc ggttcggttg
     6241 gcaaaaaaat atgatttgtt aacgatgtct gcaatcggta ctagtcagga aagtgcccga
     6301 ccagaaacat tgcggcaatt agccattatg aataaaagtt gtggtgttga tttacaacat
     6361 attggagatg ctggatacgg gggattggca ccagcagaaa atattttcga aacctcggtt
     6421 gcaattagag gaatgcgaca taccatcaac tgcgttagcc gctcgatcaa tcgctaacaa
     6481 aattaaataa aaaatggtta aattgattat ttaagtggga acatacttga gatatcagct
     6541 aacttctgtc attttaaaaa aagggactgt gacagaagtg aaaatagaca aaaaatatcc
     6601 gaacaagaga ctaggatagc agaaaaacta tcgttttgca acgaggacca actggaacgt
     6661 aggagcaatt ctcttattgt tcggatattt cgcttgttca aggacttatg tctgtagtgc
     6721 ccttttttgg taggaaaaat ggtagggaac catatcattt ttaatgaaaa tcaagcattt
     6781 caaaagttga aaaacgttga tttatcggca ttccccatcc atattatatt tacgtcttat
     6841 tcccactcca ctgttgcagg tggtttgctg gtaatatcat acacgatacg gttaacgtgt
     6901 gcgacttcat ttacgatacg gacactgatt ttttgcaaga cttcccaagg gatacgggca
     6961 aagtcagcgg tcatgccgtc aatagaagtg acagcacgga tacctacagt gtagtcatac
     7021 gtacggccat ctcccataac cccaacagag cgaatgcctg gtaggacggt gaagtattgc
     7081 cagatgtcgc gatctaaacc ggcattggcg atttcttcgc gcaagatagc gtctgattca
     7141 cggacgattt ctaatttttc ttctgtcaat tcaccaagga cacgaatacc taagcctggt
     7201 ccagggaatg gttggcgcca aacgatgctg tcaggcatgc ctaattgggt acctaattca
     7261 cgtacttcgt ctttgaacaa tgtgttcaat ggctcgatca gcttgaattg catgtcttca
     7321 ggtaatccac ctacattgtg atgtgatttg atggtttggg cagtttcagt tcctgattcg
     7381 atcacatcgg tataaagggt tccttgtgca aggaaagaaa cgccttcttc accggcaagt
     7441 ttggttgctt catcatcgaa gacatataca aattcatttc caatgatttt acgtttttct
     7501 tctggatcag acacgccagc aagttttgat aagaaacgtt ctttggcatc gacttttata
     7561 atgttcaagc caaattttcc gccaagactt tccataactt gttctgcttc acctttacgc
     7621 aacaatacgt gatcaacgaa gatacatgtt aattgatcgc cgatcgcttt ttgtaaaaga
     7681 acaccaacaa cagaggaatc tactccgcct gaaaggccta ataaaacttt tttgtcaccg
     7741 acttgttcac gaatttttgc gatctccatg tcgatgaagt tatccattgt ccagtcacca
     7801 gtacattggc agacatctag tgtaaaatgg cgcaacaagt catttccata ttcggaatgg
     7861 cgaacttctg catggaattg gattccgtac attttgcgtt ctgcattttg gatcgatgcg
     7921 atggggcaat ctttgctggt agcaacgaca tcgaaatcag tcggtgcttg ggtaactagg
     7981 tcaccatgac tcatccaaac ggtttgtttt tcaggagtgt tttggaaaag ggccgcttct
     8041 gcagaagtga tttccaattc tgcttttccg tattcacggt ttttggctgg tttaactttt
     8101 ccgcctaatt tataagtaat caactgcata ccgtaacaga ttcctaaaac tgggatcccc
     8161 aaatcaaaaa tcttttcatc aataccaaag gccttctcat cataaacact gtttggtccg
     8221 ccagaaagga tgattccttt agggttcatt gcctttactt cttctgcggt aatgcggtgg
     8281 ctcatcaact ctgagaaaac accgaactca cgaatacggc gagtgatcag ttggttatac
     8341 tgactaccgt aatcaagtac gataatttt
//
LOCUS       DACCCR010000102         8298 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCCR010000102 DACCCR010000000
VERSION     DACCCR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.109"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            47..679
                     /locus_tag="IVK93_002454"
     CDS             47..679
                     /locus_tag="IVK93_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6387701.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(681..1586)
                     /locus_tag="IVK93_002455"
     CDS             complement(681..1586)
                     /locus_tag="IVK93_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6387702.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(1671..2324)
                     /locus_tag="IVK93_002456"
     CDS             complement(1671..2324)
                     /locus_tag="IVK93_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6387703.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            2646..3590
                     /gene="mvk"
                     /locus_tag="IVK93_002457"
     CDS             2646..3590
                     /gene="mvk"
                     /locus_tag="IVK93_002457"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP6387704.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3583..4560
                     /gene="mvaD"
                     /locus_tag="IVK93_002458"
     CDS             3583..4560
                     /gene="mvaD"
                     /locus_tag="IVK93_002458"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP6387705.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4573..5658
                     /locus_tag="IVK93_002459"
     CDS             4573..5658
                     /locus_tag="IVK93_002459"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP6387706.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5655..6719
                     /locus_tag="IVK93_002460"
     CDS             5655..6719
                     /locus_tag="IVK93_002460"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP6387707.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6968..7771)
                     /locus_tag="IVK93_002461"
     CDS             complement(6968..7771)
                     /locus_tag="IVK93_002461"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP6387708.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="IVK93_002462"
                     /pseudo
     CDS             complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="IVK93_002462"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(8078..>8298)
                     /locus_tag="IVK93_002463"
     CDS             complement(8078..>8298)
                     /locus_tag="IVK93_002463"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP6387709.1"
                     /translation="ALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLIVI
                     IIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         2505 a         1740 c         1625 g         2428 t
ORIGIN      
        1 gattcccgct tctttgctat actaaagaaa aacgaggtga acccacatga aaaaaatcga
       61 acctattcca ccttctcttc ctcttcttac aaagggagag ttttatttag aagatgagga
      121 gatcttctct ggtcttctga tcaacggagg taaccaaagc tatctcgatg ccaaaaatat
      181 ggtattaaga gagagtcata tccaaaaact tacgatgcaa aaaacaggtt tacgtcattt
      241 tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc aatctggagt ggattggtgg
      301 cagtttccac caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag
      361 ttatcttcga gactgtactt tcactgattg tattgcttcg atggcttcct ttagtagtac
      421 aaacttgaaa gctgtctcct tcgatcattg tcaattagaa gacagcgaat tttatgaggt
      481 cacttggaaa aacctttttt tgtctgacaa tcagttaacc ggttcgaact ggtttcgaac
      541 ttctctgaag ggattagact ttacgactaa tttcttcaca acaatcgctt tatctcaaga
      601 ttctttggcg ggactaatcg tcaatcaaga gcaagcactt gtgatcgccc aagcccttgg
      661 tttgcagatc aaagactaga tcaacgaatc cgccgccaca gtgcaataag catccaaata
      721 gataagacca aactgatgat caaggcgatt tcccatccga aaacggaatg ggtaaagggt
      781 aactggagat tcattccgaa gaatcctgtc acaatagttg gaacggtcaa gatcagcgac
      841 cagaccgtca aaaatttcat cgtgtcattc aagttgttat tcagcaaatt attataagtg
      901 ccagagagct ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct
      961 tcgatcagtg catcgtccaa ctcttctttt tccttttctg acaacttgcg ataaatagcc
     1021 agtgccttga tttgttctaa aagtactaca ttttgttttg ttcctgtaac taagtaaacc
     1081 aaaccgatct ctaaatctga aagttgcagc agattcttgt tcgttgtttt ttctcttaat
     1141 ttcagattaa gccgctgacg ttcactattc acttcctcga ttaaaggaaa aaaggaatca
     1201 gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac tcatgtctgg catccgttcc
     1261 aaaagcgatc gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa
     1321 ccgtcttctt ttaaaataaa agcgatagga ctagtttcat aatgattatc gactttttgc
     1381 tggtgcggaa catttgatac aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc
     1441 gcccgttcat ttttatctag agaataagtc agcatctcgt cactaatgcc atattcttct
     1501 tttaagtttt taacagctag cacatcgttt tgctgtacat tgatccaaaa attacggtct
     1561 tgattaaaat agtgtttttc ccgcatactc tatcctcctg acatttgttt ccagtataaa
     1621 gaaaaaagtc gctcaaataa aggtttaccc tttatttgag caactgtaaa tcaaatggat
     1681 actttggttt ggcgaatctc tgctaatttt tcttcgatca ttttcaagac ttcttctttt
     1741 gcttgaggat cttccacaaa attatagcgg tctccatcga tttggatttt tggactttct
     1801 ttgtattctt cataccattg atcataacgt ctgttcaatt ctttgtaata atcgtataga
     1861 cttgggtcaa aggacaattg ttcatacgaa cgaccacgtt tttcgatacg ctctaacatc
     1921 gtatcaaaag atacacgaat atgaactaat aaatccggat gttttttatg ggcagcatag
     1981 ggaagttctt ccatcatgtt ttccagcagt tcatcataca cacaaacttc tgtttctgtt
     2041 gctcttccta gatctgcatt tagatggaaa agcagtgaat cttcataaat cgaacggtcc
     2101 aatacattat tgtcatcttt cattgcttgt ttgatgcttg caaaacgttt attcaaaaaa
     2161 taaatttgca gcaaaaaagc atactgttca gggtttgcat aaaataaggg caagacttca
     2221 ttatcatcaa tcgattcata aaatgcctga ctatcaaagt gttcagccat catttctgtt
     2281 aaacttgatt ttccggcacc aattgtacct gctaaaacga tcacgctcat ttacctccag
     2341 ctcacttcat cactaaatat tgtgtttaac tccaaccttt tttaaatata cccccaatat
     2401 ctagtagtgt caattgtatt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa
     2461 gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag
     2521 taaaataaag gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc
     2581 gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt
     2641 agattatggc aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc
     2701 acgcggttgt ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg
     2761 cattccttga agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa
     2821 atttagccga agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa
     2881 aacagcatga catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac
     2941 gtggaatggg atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact
     3001 tagcatttcc tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg
     3061 cccacggtaa tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt
     3121 ttacaaaagg gcatcctttt gactactttt ctttgaacat cgatgctttt ttgattgtcg
     3181 ctgatacagg aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg
     3241 aaacccagcc tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag
     3301 caaaacaagc aatcattgag aattcaccag aaacgttggc acagacaatg gatgaatcac
     3361 agtcacttct ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag
     3421 caaaagatac cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga
     3481 ttgcattagc acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg
     3541 gtgctgctga aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca
     3601 cgcgcatata ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc
     3661 cttccaatga acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc
     3721 atcttttctg atagctatat ggtagatgaa ttttatctag atggcacctt acaagacgaa
     3781 aaagcaacaa aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca
     3841 ctaaaagctt cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct
     3901 gccagcgggc tagctgcttt agcaggagct tgcaatactg ctcttaagct tggattagac
     3961 gatctctctc tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc
     4021 ggtggtttcg ttgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc
     4081 ccttccgatt ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa
     4141 aaagaagtgt ccagcagaaa tgggatgcgt cgaacagtcg aaacatccag tttttatcaa
     4201 ggctggttag attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa
     4261 gatttccaac ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact
     4321 ttagctgccc agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg
     4381 gtgcggcaat tgagaaaaaa aggcattcca tgctacttta cgatggatgc tggccctaat
     4441 gtcaaagttt tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta
     4501 tttagcaaag aacaagtaat tactgcccat gctggtccag gaattgcgat tattgaataa
     4561 agggagattt ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat
     4621 gcagttgttg aaacaggaca tccagcagtt atcgctgcag tcgatcaatt cgtaacagtc
     4681 actgtagaat ccgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct
     4741 gtacgttgga caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat
     4801 tatatccttg ctgctattcg tttgactgaa aagtatgcac aagaaaaaaa cattctttta
     4861 tccttttatg atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga
     4921 ttgggttcta gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg
     4981 ttgaatttat ctcaattgga gattttcaag attgcagcac tagccaattt agcagttcaa
     5041 gataatggtt cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca
     5101 accttcgacc atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt
     5161 gccctggatt ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta
     5221 ttgattggtt ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt
     5281 tcgagagaag acaaaatggt ggcttatcag cttttcttaa aaaacagtac agaatgtgtc
     5341 aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga
     5401 aaaaaccgac aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct
     5461 gctttgaaca aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt
     5521 gcaggtggtg gcgattgcgg aatcgtaatt gttgaccaga aatctggcat ccttccttta
     5581 atgagtgcat gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa
     5641 agaaaggaaa accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg
     5701 ataaacaaaa aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag
     5761 cagtttctga tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt
     5821 atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa
     5881 tcgtcgcaag agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag
     5941 acccttcttt agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca
     6001 ttgccaacat cggtgcaggg acttctgtcg aaagagccca agaagctatc cgattgtttc
     6061 acgcagatgc cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag
     6121 accgtgattt taccaattgg aaagtactta ttcaagagac tcaaacagcc attgacgtcc
     6181 ccctcatcgt aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg
     6241 ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg
     6301 aaaacgcccg tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag
     6361 tctcttcatt gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag
     6421 gtatccgcaa tgcctatgac atatttaaag ccctttgtct cggcgcaaat gcagtgggta
     6481 cttcaggaac cgtactgacg catctgatga atcacggcgt agaagaaact attatactga
     6541 tgaagcaatg gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg
     6601 cggccctaca ccaacaatca ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa
     6661 gagggatcga cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa
     6721 gaacactttt atcccacgct ctaaacacgt ataaacggcg gaagcaaaag caactccttc
     6781 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt
     6841 cttggagtta aacgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct
     6901 gtccttcggt attcgttcaa gccgctgatt tcacaacctc tccactaaac ggtgtactaa
     6961 agctctttta aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat
     7021 ggccgttaac gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga
     7081 ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac
     7141 gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg
     7201 gtccaattcc attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca
     7261 gcatttttaa tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac
     7321 tcatcatgga gcagatattg atgattttac cgccgccttt ttcgatcata tcccgaataa
     7381 ctgcttttga aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg
     7441 ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat
     7501 caactgaacc tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg
     7561 tcacatcgca gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt
     7621 catcaactga ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag
     7681 agcgtgcaat ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat
     7741 ctaaacgaaa catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat
     7801 tgatccattt gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag
     7861 atgaatgagt aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg
     7921 gcttgttcat tgtctacgac taaatgtttt gtttcatctg gtttgcccat catatggaac
     7981 acacgtgtat cttcattggc ataatcaaaa tagacataag cttccatacg acgttcatgt
     8041 gtatgacaag gcatcgtatt ccaagcactt cctggttcta aaatcgtata acccatttat
     8101 aaatgacagc tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga
     8161 taatgatgac aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag
     8221 ccatctgtcc tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta
     8281 gcatgattcc tagcgccc
//
LOCUS       DACCCR010000103         8296 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCCR010000103 DACCCR010000000
VERSION     DACCCR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.110"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(51..1517)
                     /locus_tag="IVK93_002464"
     CDS             complement(51..1517)
                     /locus_tag="IVK93_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP6387710.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IVK93_002465"
     CDS             1904..2500
                     /locus_tag="IVK93_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6387711.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IVK93_002466"
     CDS             complement(2558..3040)
                     /locus_tag="IVK93_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387712.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IVK93_002467"
     CDS             complement(3146..3343)
                     /locus_tag="IVK93_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387713.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IVK93_002468"
     CDS             3638..4396
                     /locus_tag="IVK93_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP6387714.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IVK93_002469"
     CDS             4456..4884
                     /locus_tag="IVK93_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387715.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IVK93_002470"
     tRNA            4930..5002
                     /locus_tag="IVK93_002470"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IVK93_002471"
     CDS             complement(5152..5523)
                     /locus_tag="IVK93_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6387716.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IVK93_002472"
     CDS             5738..5923
                     /locus_tag="IVK93_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387717.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="IVK93_002473"
     CDS             complement(6006..6194)
                     /locus_tag="IVK93_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387718.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="IVK93_002474"
     CDS             complement(6218..6481)
                     /locus_tag="IVK93_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387719.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="IVK93_002475"
     CDS             6639..6896
                     /locus_tag="IVK93_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP6387720.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="IVK93_002476"
     CDS             complement(6967..7158)
                     /locus_tag="IVK93_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387721.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="IVK93_002477"
     CDS             complement(7299..7619)
                     /locus_tag="IVK93_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387722.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="IVK93_002478"
     CDS             complement(7642..8076)
                     /locus_tag="IVK93_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6387723.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2633 a         1636 c         1314 g         2713 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacg
//
LOCUS       DACCCR010000104         8065 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCCR010000104 DACCCR010000000
VERSION     DACCCR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.111"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..881
                     /locus_tag="IVK93_002479"
     CDS             <1..881
                     /locus_tag="IVK93_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP6387724.1"
                     /translation="SVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYN
                     DYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLG
                     VVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTL
                     AMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAV
                     VLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWI
                     IGAILCLITFLLYGNA"
     gene            929..3058
                     /locus_tag="IVK93_002480"
     CDS             929..3058
                     /locus_tag="IVK93_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP6387725.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3033..3722
                     /locus_tag="IVK93_002481"
     CDS             3033..3722
                     /locus_tag="IVK93_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387726.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4130..4816)
                     /locus_tag="IVK93_002482"
     CDS             complement(4130..4816)
                     /locus_tag="IVK93_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6387727.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4952..5146)
                     /locus_tag="IVK93_002483"
     CDS             complement(4952..5146)
                     /locus_tag="IVK93_002483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387728.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5196..5636)
                     /locus_tag="IVK93_002484"
     CDS             complement(5196..5636)
                     /locus_tag="IVK93_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP6387729.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5640..6485)
                     /locus_tag="IVK93_002485"
     CDS             complement(5640..6485)
                     /locus_tag="IVK93_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP6387730.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6634..8052
                     /gene="pepV"
                     /locus_tag="IVK93_002486"
     CDS             6634..8052
                     /gene="pepV"
                     /locus_tag="IVK93_002486"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP6387731.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2480 a         1515 c         1713 g         2357 t
ORIGIN      
        1 tttcagtacc ggttgcactg ttctcattta tgggctcgat tatggctgga atctttgcta
       61 atatcgtcaa tttcaaacaa tatcaaacga tttatgctgg tttcaatcca gcggctgaaa
      121 gctatactta taatgactat ttccaaattg gtatgattgg gatgattgta agcttagttg
      181 tggtactaac ggtcgctaat atatcaatga acaaaaagaa acgctatgca atggcagcaa
      241 acgtaccagc tgaaggtggc gacgcaccaa tgatctcatg gttagccgta ttattaccag
      301 ttttaggggt cgttctttta gatcttcaaa tcatcttagg ttttattcta gcaggtatct
      361 gggcattgct gtttactgga aaattgcgcg gaggttataa agaaatctgt cgtcagtttg
      421 cgaaattatt tacagatggc gcagtcgatg ttgcaccaat ggttggtttc ttgatgacct
      481 tagcgatgtt caataattca gctgcgtatg cttctcctta tttttcagct atttttggag
      541 attggatacc gcaatcacca ttagtcttgg caatcgtatt tgctatattg acaccactag
      601 gatttttccg cggtccaatg aatctagtcg gttcaggctc agctatttta gctgtcgtgt
      661 tagctgttaa tccaacgatg tcgccagcct ttttgttccc gttatttgca attacaacga
      721 ttgctcctca gcatttagat attacacaat cttgggttgc ttggggatta ggttatacaa
      781 aagtaacttc tcgagaatat atgaagaaat ccatcccaac tggttggatc atcggtgcga
      841 tcttatgcct gatcacgttc ttattgtacg ggaacgccta gtaaacaaaa cgtcgataaa
      901 aacgattaga taaatggagg aaaagaaaat gaaacgtttt gtgcgaatgg gaattgatgt
      961 tggcggtaca catacgaaag cagtcgcaat cgataatgcg acgcacgaaa tcatcggaaa
     1021 atcttcagtg aaaacaaccc acgatgatgt ccgtggagta gctgcaggtg tcgttcagtc
     1081 ttttcaaaat tgtttgcggg aaaataatat cagtccagaa gatgttgtgt ttgttgcaca
     1141 ttctaccaca caagcgacta atgcactgat tgaaggagac gtagcaaaag tcggcgtaat
     1201 cgggatggca aaaggtggat tagaaggttt tttagcaaaa cgacaaacta gattgaacga
     1261 tatcgattta gggaataaga aaaaaataga gattgtcaat gcattcttac ctgtcaaaaa
     1321 cttaaatgta gaccgtgttt cagaaacgat ttcttcttta gaaagagaaa gagcggaggt
     1381 cttggtttct tctatggcat ttggagtaga taatggggaa ccagaacgag ttgtttatga
     1441 agcagcatct gtgaaagcga tccctacaac gatggcttct gacatcacaa aattatatgg
     1501 attgacaaga agaactcgga cagcagcaat caacgcatct attttaccaa aaatgctaga
     1561 tacggcaacc tcgacagaag actccgtacg agaagcaggg gtcaatgttt ctttgatgat
     1621 tatgcgcgga gacggcggag taatggaaat caatgaaatg aaaaaacgac ctgttttaac
     1681 catgctttcc ggtcctgctg catcagtcat gggctctttg atgtacttga gagcgtctaa
     1741 tggtgtttat tttgaagtag gcggaacaac aaccaatatc ggggtcatta aaaatggacg
     1801 tccagcaatc gattattcaa ttgtaggtgg ccacccaaca tatatctctt cattagatgt
     1861 tcgggttcta ggtgtggcag gcggttcgat ggtacgggct aatcagtcag gaatcatcga
     1921 tgtcggtcca cgttctgcac atattgccgg tctagattat gcagtattta cggagacaga
     1981 aaaaatcaaa ggtcctaagg ttgaattttt ctctcctaaa gaaggagacc ctgctgatta
     2041 cgtcaaggta gtgatggaag acggagaaga agtgactatt acaaatacat gtgcagccaa
     2101 tgttcttgga ttagtgcaag aagaacattt ttcttatgga aatgtcccat ctgcacgaaa
     2161 agccatacag gcattagcag actattgtca tacgacggtt gaagacatcg cagagcagat
     2221 catggaaaaa tcctatgcta agattgaacc ggtgatttta gaattggcag acaagtatca
     2281 cttggagaaa gatcagattt ctcttgtagg tgtagggggc ggcgctgcct cattgattac
     2341 ttactttagt aataaaatgg gtgtgaaata ttcgattcct gaaaacgccg aagtgatttc
     2401 ttctattggt gtagcacttg caatggtccg agatgttgta gaacgtatca tcccttcgcc
     2461 aagtaaagaa gatatacgat cgttgaaaaa tgaagcgatg aataaagcga ttgaatcagg
     2521 cgctacacca gaatcgatcg aagtccacgt agaaatcgat ccacatactt caaaagtaac
     2581 ggctatcgct actggttcta cggaagtgaa agcaaccgat ctaaccaagg agatcacaac
     2641 agaagaagca ctagagcttg ctgcagagga tatgcgatta aataaaaatg aagtttgctt
     2701 attggaaaat acacctttct tttatgtatg cggcgaacag aatcgttcaa aaaatgctgg
     2761 aagtcttcgg atcatcgatc aaaaaggatt tatcaaggtc caacgtggac atgcttcatg
     2821 tatgaaaaca actgcagcca attatatgac agctgtcgaa caactttggg aagatatggc
     2881 agtttatcaa actgaattga ttgcccgacc agaattttat ctctgcttag gagcaagagt
     2941 ctctgatttt acagcgacgg atctggaaca actacagctt ttgatggatc tggaagtatc
     3001 tactatggaa ccggaggaag aagtgatcgt cgttgccgga aatatcaaac aaacataagt
     3061 tagaagagtt actaaacgaa ttacaacaga tccccgaaga aatttgggga ttctaccagt
     3121 ttcggcgaga tttattttgg aaaaagattc ctttgtccaa gcagaaaatt cttatacaac
     3181 aatcaatcga ttgtggtatc gaaacggcat gtagcatcaa aaaaaagtac ccttttgccg
     3241 atgtaggaga aatctgtgaa caaatggctg ttccaatcgt ttcctgtgaa tcagagcaaa
     3301 taaacgaacg aatcactttt gcaacttatg cagaagatga agggatccga ttgatgacgg
     3361 agccgttaga aaaattaaaa tgttcaggac tagcttcgat ctctaaagaa acagcgcaag
     3421 ctttgattat tggacatgaa ctatttcacc acatcgaagc ttctgttaag ggaatttata
     3481 cgcaaagcga aaaaattgtg ctgtggcggt tgccttttta tacacatcaa tcaaacattc
     3541 gtgcattgag cgagatagca gcgatgagtt tttcaaaaga gatgaatcaa agcgattttt
     3601 cgccttatgt attagaggca gtcttacttt ggccatataa tgaaattcgt agccaaggga
     3661 tcttagaaga aataaaagaa atagaaaaaa gatgtacgga gtatgattta gattacaaat
     3721 aaagagactg tgacaaaagt cgtgtcacag tccttttttt ccgaataaac ggtgttctcc
     3781 tgtcaactcc tcggtcacaa gtcaaaatat tcaacaaaca tgagaagctg ttttttgaaa
     3841 ttgttccttg tgactcggag atgaacggca ggctcacgac ctcttggaaa aaggtgttca
     3901 aaagcttgca tctgcggtaa taagaacagc caagttaaaa acataagaag ctatttttac
     3961 ttggctgttc ttattacttg atgcagggca gctttttcac aacctctcaa taaacggtgt
     4021 gcaatcagct gaccctcggt attagctcaa aaaaaacggc aaaatatgag gaactatttt
     4081 gccgtttatt tctctaatac tcaggtcaaa acgctgattc caaaacctct tatttgatgg
     4141 gtctgtcctg aatatcttca aaataatcgg ggtaattatg aatcacgtat tcttgctcct
     4201 cgataatcat atgtaaatgc atcgtgcaaa gaaatccgac ttcatcaaag ttatgatcaa
     4261 tcattgattg aagcattgtc tgatgttcat ctaatactcg accccagtct aattctgaaa
     4321 tcgtcaaacg taaccatctg aaacggttca agtgcgtact ataactttct agccaatccc
     4381 aaatttctcg ttttccagca atttcataac aagttcggtg aaaatcagta tccaaatcaa
     4441 aaaaagcttt tttattttga gcgatcactg cctgatcggt ttgttctagt attttttcta
     4501 gtttagcttg atcttgcttg gtcattttcg cactgcattc ttgcaggatc ggtttttcca
     4561 acttttcccg cgtataacgg gctaatgaag cagatcggat atcgattttt gaaacataag
     4621 tgccggactg tggaatggtt tttacaagct cttgattgcg cagttggatc agtgcttctc
     4681 gaatcggtgt acgcccgata gctagttgtt cttctaacac tttatcggag attttacttc
     4741 cgggtgctaa ctcggaataa atgatttgtt tacgtataga ttcataagct tgctgttgca
     4801 aattattgat aaccatttaa ccttcctttc tatgcagata taatctatta taacataaat
     4861 cgcttacaaa tcgtgtgggc ataaaaacac agtctgcaaa aaaagccctg ataggcatgc
     4921 ctattagagc ctcgaaaaga aaaatattga atcaatctgg tcgtacctct tggatcggtt
     4981 tgacacgacc gccgattctt ttagcaaaag cttcggattc ttttttgtct ttgaaaatga
     5041 gcagattgtc taagtttttt tcccgagtgc gacagccagt tttatcgatg taaccgtgca
     5101 ttactttaac gacatacatg gggatcacct gcttttttct tttcattata cgaactactc
     5161 gacacaatac aagaaaaatg cttagacggt ctttattaag aatttacatc atccaaagaa
     5221 gcgtacgctt ggaataactg ttctaactcc ggattgactt tgctcagctt cactaatcgt
     5281 tgcgaatcag aaagtaaaaa acagtgttta ctactgccag tagtttttaa ttttttgtct
     5341 tcagttccca caatttcgta gctaaaatcc aaccgtgttc ccgtatactt ttggatagtt
     5401 gggtggatgc tcactttttc tccgtaatgg atcatttctt tatagctgca atttacttcc
     5461 agaacgggga tgatgatccc agattcttcg atttttttat agggaaaccc tagatgtacc
     5521 aataaggcta cccgagcctc ttcaaaccag cggatataat tgctgtgatg aataatcccc
     5581 atttgatctg tttcataata aaaaggttct cgcacataac ctgtaaattt ctccatgact
     5641 catgcctcgt tttccattga ttttttacaa aaataattca tccacttcgg aagtgcttcg
     5701 ctgatttgtg tcggcaaaac aatatattgt tcttttgcca gctggtctcc aatgaaacta
     5761 tgaagataaa cagctgctgc aatcgtttgg tcattttgag gaaactgggc taaaaatcct
     5821 gtgatcatcc cagctaaagt gtcacccatg ccgcctgttg ccatagcagc agaacctaaa
     5881 ggattggtgt agaagtgctg gtgaccataa atttttgtct catgactttt caaaacgagc
     5941 acagcaccga tttcctgttg cttctttttg ttttctttat cccgttgttc ttgtatcggt
     6001 agagaactca accgttgcca ctccatttga tgaggtgtaa gtacagtttg ttcgggatag
     6061 ggtagcaaaa gatgattagc aacaagtaaa cttaaagcag agccatcaat caccagccat
     6121 tgttcttttg attggttttc tagtacttgt ttgaaacgtt ccaaactctt agaagaagta
     6181 cccaatccgg gaccaatcaa gagtacatct gcttgcttta ttacggaaag aaactggtca
     6241 gtatccgtcc aatctacaat cattgcttct ggcagatgac tgtgaagagc actccgattt
     6301 ttttcatcag ttactacagt cgtgagaccc gctccactgt gtacggcagc taaagcactc
     6361 ataatgattg ctccgccata ttgttcattg ccgccaatca aaacgatacg tccaaatgtt
     6421 cctttatgtg aaatctttgg acgaggtgtg atgactcttt tcactagttg ttcgtttatc
     6481 agcatcgaaa aacctccttt tactcattat agcgtatatt ttttttagag aaaaacagat
     6541 aagaaaagga attgtcagat tttttgaact atttttttct gtaatttgtg gtatcatgaa
     6601 cacgaagact taaataagtg gaggattaac tttatgacaa ttgattggca aaaagaagta
     6661 gaagcacgta aagaagactt gcttgaagac ttgaaaaatc ttttacgtgt caacagcgag
     6721 cgcgatgatt caaaagtaac accagatgca ccatttggac ctggacctcg cgacgcattg
     6781 aaacatatgt tagcttatgg cgaacgtgat ggatttaaag taaaaaatgt cgataactat
     6841 gcagggcata ttgatcttgg cgaaggagac gaaacgctag ggatatttgg acacatggat
     6901 gtcgttcctg caggagatgg ctgggacaca gatccttatg aaccagtaat caaggatgga
     6961 aagatctttg cacgcggatc aagcgatgac aaaggtccaa gtatggcagc ttactatgcg
     7021 atgaagatca ttaaagaact tgacttgaaa ctatcaaaaa aagttcgctt cgtagtcggc
     7081 agcgatgaag aaagcggctg gggcgatatg gcttattatt tcgaacatga agaagaacca
     7141 gattttggtt tctcaccaga tgctgaattc ccaatcatca atggtgaaaa aggaaatgtt
     7201 tctttagctt tacgcttcaa aggcgacaat gcaggagatt atgtcttgaa atcttttgtt
     7261 tccggacttc gcgaaaatat ggtgccagga acagcaactg cagcacttca agtaccaagt
     7321 gcagatgcag ctatcgcgat ggaagaagca ttttaccaat tcatcgaagc aaatccagta
     7381 agcggaacga ttgaagcgga caatacgtat gtaaaaatcg aattagttgg taaaggcgca
     7441 catggtgcaa gcccacaatc tggtatcaat gcaggttctt tcttggcatt gttcttagat
     7501 aactatgaat tcctaggatc tgctaaacaa tttatccatg tagcagctgc ttatgtccat
     7561 gaagattttt atggagaaaa actaggagtc gcttatgaag atgaaaaaat gggcaaattg
     7621 acaatgaatg ctggactatt tgcttttgaa gaaaatggta cagaggaagc gaacttcatc
     7681 aatatgaatt tccgttttcc aaaaggtgta acagtagacg gattgcaaag tgacatcgaa
     7741 caaacagtgg ggcaagaagg cgcaacagtt actcgaggtg ctcgcgtaat ggaaccacat
     7801 tacgtaccaa tggatgatcc attggtggct acattattgc aagtctatga agaccacact
     7861 ggagaaaaag gctacgaaca aatcatcggt ggcggaactt acggacgttt gttgaaacgt
     7921 ggcgttgctt atggagcgat gttcccagga tatactgata caatgcatca agcaaacgaa
     7981 tttatgagtc ttgatgactt gttcagagca acagcaatct atgcagatgc catctatcgt
     8041 ttggcgaaat aaaaatacta aaaaa
//
LOCUS       DACCCR010000105         8008 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCCR010000105 DACCCR010000000
VERSION     DACCCR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.113"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(296..1024)
                     /locus_tag="IVK93_002487"
     CDS             complement(296..1024)
                     /locus_tag="IVK93_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6387732.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IVK93_002488"
     CDS             complement(1002..2576)
                     /locus_tag="IVK93_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6387733.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIAPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IVK93_002489"
     CDS             2805..3743
                     /locus_tag="IVK93_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6387734.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IVK93_002490"
     CDS             3755..4666
                     /locus_tag="IVK93_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6387735.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IVK93_002491"
     CDS             4690..6162
                     /locus_tag="IVK93_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6387736.1"
                     /translation="MKKNMQKYLIGGVSVLILGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6214..6825
                     /locus_tag="IVK93_002492"
     CDS             6214..6825
                     /locus_tag="IVK93_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6387737.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6855..>8008
                     /locus_tag="IVK93_002493"
     CDS             6855..>8008
                     /locus_tag="IVK93_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP6387738.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEG"
BASE COUNT         2543 a         1206 c         1583 g         2676 t
ORIGIN      
        1 atttaaataa ctacatccat ctattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gcgcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt acaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgtttc tgtactaatc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgacga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttta ctaaatgaaa
     6181 agaatagact aagcagttag tggggggatt caaatgaacg aaaacaaatg gaccaaagga
     6241 tttaattggg tttttcaatt gttattacta aactttactt attttatact aatttgttta
     6301 ggatttggaa ttttaggttt tttccctgct acgatagctt tgattgctgg cgttgtgaat
     6361 cgaaaaaaat atggcttgca agagtctgtg aagttcatga gtaaaatcta taagaaaaat
     6421 tttgttagat ataatctata ttgctggtca gcaactttgt tgttggccgg caatattact
     6481 acgatctggc tttgttggca gcaaagcgca ccattgtttt atttattaag tacattatta
     6541 tcggtaatta cactgttcct agtctttgca atgttgaatt gggtcgtgat caaaagtctt
     6601 gggttgtgga tgatgggaga ccgactgatt gactatttgc ttttcacagc aacgatgttg
     6661 ccaacggtag tcatgcaagg cttggtgctt ggaatttatt tccgaatatt gatgattttg
     6721 cctgtgattt ttctgtttgg cggaacagct gtaacggtaa tattgattag taaattaact
     6781 gaaagagcgt tagcaaagtt gccttgtcag atacaatcgc actaattttt tcacaaaaag
     6841 gagtatttct ttctatgaag ttaactgata aacaagtagc taatatagcc tttatccagc
     6901 aacttgagat tccattgaca caaaaagatg aagaacaaat agacttgatt tttactgaca
     6961 caaaggctaa tgtgcgacaa atcaagatta ctaaaaatga gcagcaacga atctgcttgt
     7021 cttattcaca tgaagacttg ctgtatcgag gattgtttat ctgccttcca cgttttttag
     7081 ctcaaggagg agagatgcca tacattgaag aaacctcgat cattgagacc agttattcat
     7141 tagatgcttc tcgtaatggc gtgatgaata aagagactct aaaaaaattc atttcttact
     7201 tagcgttgat ggggtacaac acattgtata tttacacaga agatacctat gaagtagaag
     7261 accttccata ttttggttac cttcgcggag cgtatacaaa agaagaagtg aatgaaattg
     7321 ttgaatttgc ttctggtttt ggcatagaag tagttccatc gatccagacc cttgctcact
     7381 tgacccaatt tttgaagtgg tatgctcaac aagaactgat ggatgatcaa aatactctac
     7441 tgatcggtga agaaaaggtt tatgcggcaa tcgaaaagat gattattgcc tgcaaagaaa
     7501 tgtatcagac agagcgtatt catttgggaa tggatgaagc ctatcaagca ggtctgggac
     7561 gatatttaag cctgtatggg tattgtgatc ggacaacttt aatgattcaa catatggacc
     7621 gtgtgttatc aattgttgat aaatataaga tgaaaccaat gatttggagt gatttcattt
     7681 ataagctatt agatgaaacc ggtaaagata ggtattacaa tcctgatttt aaactcaaag
     7741 cattagcagc taaaggatta ccagcaggat taacctatgt tcattgggac tatggttcag
     7801 atgaagtttc ctcctatgag aaagtaattg ataaccattt gaaattttgt gacattgatc
     7861 attacgtttt agctagtggt gcccatatct gggggagact agccccgaat catgggaaaa
     7921 cagaagcaat tgtttctgca gctgtaaaag cttctaaaaa taaaggtgta cgttctgtga
     7981 tgttaactac ttggggagat gaagggca
//
LOCUS       DACCCR010000106         7991 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCCR010000106 DACCCR010000000
VERSION     DACCCR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.114"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..657
                     /locus_tag="IVK93_002494"
     CDS             <1..657
                     /locus_tag="IVK93_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6387739.1"
                     /translation="FKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAE
                     VIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQER
                     IDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISEL
                     EKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV
                     "
     gene            complement(1360..1575)
                     /locus_tag="IVK93_002495"
     CDS             complement(1360..1575)
                     /locus_tag="IVK93_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP6387740.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1594..1830)
                     /locus_tag="IVK93_002496"
     CDS             complement(1594..1830)
                     /locus_tag="IVK93_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP6387741.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2088..4241
                     /locus_tag="IVK93_002497"
     CDS             2088..4241
                     /locus_tag="IVK93_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP6387742.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4210..5625
                     /locus_tag="IVK93_002498"
     CDS             4210..5625
                     /locus_tag="IVK93_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP6387743.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            5658..6692
                     /gene="sppA"
                     /locus_tag="IVK93_002499"
     CDS             5658..6692
                     /gene="sppA"
                     /locus_tag="IVK93_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP6387744.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            6694..7215
                     /locus_tag="IVK93_002500"
     CDS             6694..7215
                     /locus_tag="IVK93_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP6387745.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7212..7598
                     /locus_tag="IVK93_002501"
     CDS             7212..7598
                     /locus_tag="IVK93_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP6387746.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2824 a         1160 c         1433 g         2574 t
ORIGIN      
        1 tttaagatag tattgaaaac acaaagtccg ttagaagtta aaaaatacct caaacaattc
       61 cgaccaaagc aaggaatata tttcttagat attgatttaa atcatgaagt taacggtata
      121 gaattagcag aagtaatcag aaaatatgat gttcaagcaa aaatcatttt tacaactact
      181 catgatgaga tgttacccgt aacaataaaa agaagagttg aaacgttagg atttgtaaca
      241 aaagatcaaa cactagatga gtatcgaaac gagattgttg agttattgtt attagcgcaa
      301 gaaaggatag atgcaacaaa acaggttacg aatcaagcat ttgttttttc cataggttct
      361 caaacattta cttttgatat aaatgacatt tattttgtag aagcttcaaa attaccacat
      421 agattatcat tgtgtactaa agatgggcag tacgaatttt atggaagaat tagtgagctt
      481 gaaaagaaat atcctatgtt aacaagaatt agtcgagcgt gtttagtaaa tatctttaac
      541 gttaaagaaa tagattttaa aaaacgttca ctatattttg attcagagtt ggccagaaac
      601 tttactttag ggaaagcaca aaaaattaag gagaagttga aagaaagcac tgtttagtgg
      661 ttttttcttt agggagattg catttttcta ataaaataga ttccttcact agtgcaaatt
      721 tttctgaatc ctattaaagt tgtaccaatt tttatagctg ttattagtta cgtgcataac
      781 agcaatatta gttactggag gaatatttct atggtctgga accaaaaaag ggccaaatga
      841 atagttcttt tcttgttttt attaagtacc ataaagggga attaaaatta aatgtatggt
      901 ggaagatgcg aaagatttgg caaattctac taatttttag aaagtgtttt tcatttttta
      961 gttaaaaaat ttgtcctgtc aagtgcaatc ctatgattgt acttgatagg atttgagata
     1021 attactttcc tgtttttata aatttaactt gcaggaacag gctttaatgc acatccgaca
     1081 agtacattac caatacctcc tgtaacggcc gaaataggtc ctcctgcagc accaactaca
     1141 gcgctgcaag tccttttgta aaaatatctc taccattaca tttccaaaga atttttatct
     1201 gactttaata aaagataaaa atttaatata attataaaaa ctagcacaag aagctttagt
     1261 aaaaagttct gtccattcca aacttttgaa aagataaaga acatacctat gttcgtaatc
     1321 gacacaaacc aatcttgagg tttgatattt tttaattgtt tacgcaccgg tccacatgct
     1381 gaatccagca cctaataaag ctccacctac accacaagca gctattgcat aaggtcctcc
     1441 ggctgaacaa agttttactc ctgctgttgc cccagaaaaa gtagtgccta aaactcccac
     1501 ggcactccca ccttgaatat ttttcaactc ttctgaagat aatataaatg ttgtttttgt
     1561 gtttttaata tgcatactac ttattctcct tttttatttt tttcgagcat cccagccagc
     1621 tgcagcacct ccagcgacag ctccatataa ccctccaaca acaccgcaag ctggtccgca
     1681 aattgctgtt cctaaacctg caccacctac tgcccctgtg gtaagtccac ctacaacttg
     1741 ccccaatttt ccccaagcat tattgcctcc accaacagtt tgtgcatttc cacaatactt
     1801 agttccttta cattttgcat gtttaaacac gcttctttaa ataatatttt ttacggtgct
     1861 actgtaacat acgtatttga ttaacagtaa aaatcttcct gaaacttatc aagtttattc
     1921 ttgaatagta aaatttccaa aataaatttg attcaggaat gaaaaagtta gtgttcaaga
     1981 actgttttca aagtttttgt ggaattatga gacaatattt tcactgtatt aaagctatat
     2041 tgagaatttt tctataaaaa ttttttagaa ggagttgtgt tagataagtg ataaaatcat
     2101 ttccttttgt gcagcaacaa gatgaaaaag attgtggtgt tgcttgcctt tcgatgattt
     2161 taaaatattt taaaactgat atcccgattc ataaattacg ggacctatct gggactgatt
     2221 tagagggtac ttcagcattt ggattgaaaa agacatttga aaaattgtct tttgattgta
     2281 tggctattca agcagatagt gagatctgga aagaaagaga tcttatctta ccattaattg
     2341 cacatatatt ggttgataac agctatatgc actacgttgt aatttataaa acgaagggaa
     2401 acgtactatg gattgcagat cccgctaatg gaaaaaatag aaaaacgatt gaagcgtttc
     2461 aaaaagaatg gacaggtatt cttttattac ctatgcctag agaaacttat attcctatta
     2521 aagaaaaagt agctggatta agttcttttt tgcctataat ttggaaacaa aaaggattag
     2581 tattccacat cgttttagca tcgattttca ttacgttttt tggcatagga agctcatatt
     2641 attttcaagg tattcttgat tttttcatcc ctaatcaagc acgttcaact ttaaatataa
     2701 tttccattgg cttgatcgct gtctatttat ttcgggtatt gtttgaatat agtcgtagtt
     2761 atcttcttat tattttagga cagcgaatga gtatggcaat tatgcttcag tatttcaaac
     2821 atgtgttaac tttaccaatg aatttttttg ctacgagaaa atcaggggaa attatttctc
     2881 gttttcttga tgccagtaag attattgatg ctttagcaag tgctacgtta tcaatatttt
     2941 tagatattgg tatggtgttg cttgttgggg ttacgttagc tattcagaat agtacgttgt
     3001 ttttaatcac tctatgctct ttgccttttt atgtagttgc aatattcgct ttcgtgaaaa
     3061 gctatgaaaa atcgaacagg gaagaaatgg cagccggtgc agctgtaaat tctagtatca
     3121 tagaaagtct taaaggaata gaaacaatta aagcatatag cggtgaagag aaagtatata
     3181 accgtataga ccatgaattt gttaaattaa tgaaaaaatc atttcgtacg gttacgcttg
     3241 ataatattca acaaggaatt aaacatttga ttcaattaat tagcagtgca ttaattttat
     3301 ggctaggttc ttattttgtt atggataata gaattagtct aggtcaactt atcacataca
     3361 atgctttact agtatttttg acagatcctt tacaaaacat tattaattta caagtgaaaa
     3421 tgcaaacggc tcaagttgca aataatcgtt taaatgaaat tttcgcgatt gattcagaaa
     3481 aatcgaacca gtcttttgaa tatatgcttt ctgaacaatt atttcaacaa gatatcactg
     3541 taaaaaacgt ttcattctcg tatagcatga aggccccaac gttgaacgaa atttcatgta
     3601 caattccttc gcacagtaaa acggctatag ttggagttag tggttctgga aaatcaactc
     3661 ttgcaaagtt atttgttcgt ttttatgaag catcagaagg atcgatccat tatggaaaca
     3721 ttaatcactt agacattcct tttcagatat tacgcgatcg agtgacatat ttgcctcaag
     3781 agtcattttt ctttagtgga acaatcattg agaatcttat attcggttta gatagcccac
     3841 caagtttcga acaaataata gaagtttgtg agatagtcca actaaatgaa tttattaatc
     3901 aacagccatt acgttacgag acattgttgg aagaaggtgg aaataatctt tcaggagggc
     3961 aacgacaacg attagcctta gcacgggctt tattaaaaaa tccggacata ttaattttag
     4021 atgaagcaac tagtggatta gataccttgt tagaacatgc aattatgaat tatctcctca
     4081 atttaaaaga aaaaacgatt atttttattg cgcatcattt accaatagca aaagcatgtg
     4141 atcaaatttt agttctccat gaagggcatt tggtagaaaa aggaacgcac gatgaacttc
     4201 gttttgccga tggtatctat caaaaattat gggaaatcta ggagagggaa acaatttatg
     4261 cagaataata attggttcga acattctagt atctatacta aacaacatca tagcttctac
     4321 cgttgggtgt tataccctgt aattcttttt ttatgtatta ttggattgtt tttaaccttt
     4381 gctaaaaaag aggttgttat tcggacaacg gctcaattga accccgaaaa aatagagaag
     4441 ttacaagtgc cattagaagc taaaataatg gaaaacttcc taaaagaaaa tcaatttgtt
     4501 cataaggacg atgtgttggt tagattggat tgttctttaa tagagaatga aaaggctcag
     4561 atagaacaag aaaaccaacg tattactcaa caaataaaga tggctcagct atttattgaa
     4621 agtataagta aaggaaaaaa tttgttttca acggatgaca gttttggcta cagtaatcaa
     4681 ttaaagagca tgttgtcaga aaaagaatca ctccgctacg ctttgaagca aagtgaatta
     4741 aatgatcaaa agcaattaga agtatacgaa aagacaaaaa gacaactaga aaaacaaatt
     4801 gagagttcag atagtaaatt acaagaatgg caacaagtac aggtagcttg gagtaataat
     4861 caatcattaa aagatttttc aaaagaaatg atggcaaact atgagaattg gcaagaacaa
     4921 ctaaataatg tttctgatga tcaaaaaaat caagtgaaac tgacaatttc agcaagcata
     4981 aatgaacaaa ttgagcaact aaaaaaagaa gtagaacagt atcagtcaga aaaagctaaa
     5041 ttagttaaac caactacttc tgagaatgac agaattagtc aaacggaaaa aggaaagcaa
     5101 gagctagaac aagcaataac aactgtaaaa caaacagtta cagagctaaa tgaaaaacaa
     5161 gagaagaacc agtcagttat caaaagtttg gacgatcaac tttcaaaagg aatactaaaa
     5221 gctcctgtat ctggaacggt tcatttaaat gaagaaacaa agggacaagc agaactagcc
     5281 aaaggaacag tgcttgcaga aatttatccg atccacgaaa aatcaaaaat gaaatttaca
     5341 gcacttttac ctgcgaatga aagtacctat attaaagaag ggatgaaggt tcattttaaa
     5401 ttggatcaaa aaggtggggc tccgattacg atcgacggaa ctttagatga aatttcagaa
     5461 aattctactg ccactgagaa aggtgttttt tatgcagtga aaggaacgtt aaaaacaact
     5521 caatcgtttc cgtttcgtta tggattaact ggagagttgt ctttaattgt aggagaaaaa
     5581 acatattggc aacaaattgt cgatgtgttt ctaaataaaa aataattatt tttactaaga
     5641 atataggagg agtttatatg aataaaagaa gatggattgc tgtgggagta gcgtgcggat
     5701 tattgttact atccattatc gtcgcgctaa ttccaggaaa agataaagag gaagcatcaa
     5761 atacaaccct aacaggaata aataagattt tttacggatc taacgaaatc acagaagaaa
     5821 cgttagaaga aggcgcatca aataaaaaga ttgtcaaact atccgtcaat ggagttattg
     5881 cagatacagg tgaaagcaat ctttttagta gggaacaata taaccatcag aattttttaa
     5941 cacagctaaa gaaaattcaa gaagacaaag cggttaaggg agtgttatta gaagtaaatt
     6001 cacctggggg aggaatatat gaaagtgctg agattgctaa agaaatggca aaaattaaaa
     6061 agctagatat tcctatctat actgcattta aaaatacagc ggcaagtgga gggtactaca
     6121 tttctgctgg ttcagataaa atatttgcta cggaagaaac aacaactgga tcaattggtg
     6181 tcattatttc tggtttaaat tattctggct tattagaaaa acttggcgta actgatgcga
     6241 cgtataaaag tggtgcatta aaagatatga tgtcacctca acacaaacct tcagaggaag
     6301 agaataaagt tatacaggaa tttgtgatga gtgcttatga tcgttttgtc aatgttgtag
     6361 caaaaggaag aaatatggat actaatgcag tgaaagaact tgcggatgga agaatttatg
     6421 atggtaacca agcagtagaa aatgggttgg tggatcaaat tggttactct gaagatgcat
     6481 tagattccct aaaaaaagag aagaaattaa ctgatgcgac ggttatagaa tataaaaatg
     6541 acacaactgg ctttgcttct tcttggttag gtaataagat tgctgaatgg caagggctaa
     6601 aggcaactac atcagatcgt gtaatttcag tttttgaaaa acttggattt tctgatactc
     6661 caaaaccaat gtattactat ggagggctat aaaatgaata agaagagtgg cattgaaact
     6721 ttgcctaatt atgtatttgc gggattttgg ttacgatttt ttgcttttct tattgattta
     6781 atttgtatta gcatcatcac ccatgctact ataggtctct tttacaaaat catgggactg
     6841 gaatctgaca aatccattat tactttttat ggtatagcgt cattagctat ttatttaacc
     6901 tattttgtac ttttgactaa gctgaattca ggacaaacaa ttgggaaaat gatttttggg
     6961 ataagagtag tgagctttga ggaaaaagag ctaagttggc aaactatctt tattcgtgag
     7021 tgtatatgta ggtttatatt aaaaaatttt gtatttcaaa ttgggtattt ggtggctgct
     7081 ttcacgccca agaaacaaca tattggggat tatttttcta acacgtcagt ggttgttatt
     7141 cgttcattag aagctattaa ctatggtcaa aaaatttcta attctaaaac acttaatctg
     7201 gagagaatta aatgaataag agaaaaacag agtttatttt gattttaatt agtggaataa
     7261 cagggatttt atgtagtctt ttagcattga tgggtggtat gtttctatct tctattgatt
     7321 caactacttt aaatactaca gaactaacca gtaaggatat agatatgtta gtcaatctaa
     7381 gcgggaatgt tgttttattt ggaggaatta ccctagtttt ttcgataatg attttcataa
     7441 taggtttttt agtaagaaaa aatcaaaaaa cagtggtcta tggtattagt ttactcattt
     7501 tatctattat tccatttttt cttttaacat tattgtgggt aattcctgga gttttaggta
     7561 ttattgcggg attgatgctt ctaataagaa aatcttaatt aggcaatgtg tagacaacgt
     7621 gaaacgatta atcaattaaa agctaaatac gttttgagaa atgataactt gtagtcgatg
     7681 gaagacatgt agataaaaaa actacgtgtc ttttatttat tctaattttt tcgaaaaaaa
     7741 gacactctct gattgttgta ttaatggaaa aggggaaagc agcaagtaaa tttagttttt
     7801 ctattaaata ttcttcactt tttaaccttt tatagtactt agatttaatc ctatatgttt
     7861 agagagttca ttggcagcat gattttgcaa agcttctcga attattgtta tattagaaac
     7921 tttactgttc ataattatgc ttctttttta actcaaagtc caaagggcta cagtggactt
     7981 tgagttaaaa a
//
LOCUS       DACCCR010000107         7760 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCCR010000107 DACCCR010000000
VERSION     DACCCR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.116"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IVK93_002502"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IVK93_002502"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP6387747.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IVK93_002503"
     CDS             complement(2032..3273)
                     /locus_tag="IVK93_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6387748.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IVK93_002504"
     CDS             complement(3291..4214)
                     /locus_tag="IVK93_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6387749.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IVK93_002505"
     CDS             complement(4435..6927)
                     /locus_tag="IVK93_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP6387750.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IVK93_002506"
     CDS             complement(6934..7401)
                     /locus_tag="IVK93_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP6387751.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IVK93_002507"
     tRNA            complement(7537..7610)
                     /locus_tag="IVK93_002507"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="IVK93_002508"
     tRNA            complement(7668..7741)
                     /locus_tag="IVK93_002508"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DACCCR010000108         7596 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCCR010000108 DACCCR010000000
VERSION     DACCCR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.118"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(135..425)
                     /locus_tag="IVK93_002509"
     CDS             complement(135..425)
                     /locus_tag="IVK93_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387752.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IVK93_002510"
     CDS             complement(543..803)
                     /locus_tag="IVK93_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387753.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IVK93_002511"
     CDS             complement(940..1353)
                     /locus_tag="IVK93_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP6387754.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IVK93_002512"
     CDS             complement(1809..2294)
                     /locus_tag="IVK93_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387755.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2678)
                     /locus_tag="IVK93_002513"
     CDS             complement(2433..2678)
                     /locus_tag="IVK93_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP6387756.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="IVK93_002514"
     CDS             2871..3671
                     /locus_tag="IVK93_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6387757.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..5558
                     /locus_tag="IVK93_002515"
     CDS             3690..5558
                     /locus_tag="IVK93_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6387758.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5551..6042
                     /locus_tag="IVK93_002516"
     CDS             5551..6042
                     /locus_tag="IVK93_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP6387759.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6030..6584
                     /gene="srlA"
                     /locus_tag="IVK93_002517"
     CDS             6030..6584
                     /gene="srlA"
                     /locus_tag="IVK93_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP6387760.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6602..>7596
                     /locus_tag="IVK93_002518"
     CDS             6602..>7596
                     /locus_tag="IVK93_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP6387761.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1443 g         2233 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     5101 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     5161 aagaatattt aaacaaatta gtcgctcctt tgatcagagg ctattatgtg gatgaagaat
     5221 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     5281 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     5341 ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg
     5401 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac
     5461 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     5521 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     5581 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     5641 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa
     5701 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     5761 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     5821 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     5881 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     5941 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     6001 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     6061 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     6121 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     6181 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     6241 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     6301 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     6361 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     6421 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     6481 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     6541 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     6601 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     6661 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     6721 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     6781 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     6841 aatttatcct aaaaaaggga taccaaccat caatatcgtt cccacaggaa aaagtggccc
     6901 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     6961 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     7021 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     7081 agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     7141 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     7201 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     7261 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     7321 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     7381 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     7441 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     7501 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     7561 cattgcttgg gtagcaagtt ttggattata tagctg
//
LOCUS       DACCCR010000109         7535 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCCR010000109 DACCCR010000000
VERSION     DACCCR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.119"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            381..944
                     /locus_tag="IVK93_002519"
     CDS             381..944
                     /locus_tag="IVK93_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6387762.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1223..1510)
                     /locus_tag="IVK93_002520"
     CDS             complement(1223..1510)
                     /locus_tag="IVK93_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6387763.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1500..1829)
                     /locus_tag="IVK93_002521"
     CDS             complement(1500..1829)
                     /locus_tag="IVK93_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6387764.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2134..2463
                     /locus_tag="IVK93_002522"
     CDS             2134..2463
                     /locus_tag="IVK93_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387765.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2750..3235)
                     /gene="vanZ"
                     /locus_tag="IVK93_002523"
     CDS             complement(2750..3235)
                     /gene="vanZ"
                     /locus_tag="IVK93_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP6387766.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3388..4299)
                     /gene="vanY"
                     /locus_tag="IVK93_002524"
     CDS             complement(3388..4299)
                     /gene="vanY"
                     /locus_tag="IVK93_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP6387767.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4727..5335)
                     /locus_tag="IVK93_002525"
     CDS             complement(4727..5335)
                     /locus_tag="IVK93_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP6387768.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5341..6372)
                     /gene="vanA"
                     /locus_tag="IVK93_002526"
     CDS             complement(5341..6372)
                     /gene="vanA"
                     /locus_tag="IVK93_002526"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP6387769.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6365..7333)
                     /locus_tag="IVK93_002527"
     CDS             complement(6365..7333)
                     /locus_tag="IVK93_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP6387770.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2297 a         1504 c         1263 g         2471 t
ORIGIN      
        1 ttataatatt ctattcctta gtatctcaac attatttact ttataaaacg aataaatttt
       61 gtagttctaa ttcaaatatt gcatgttata tctaatatgg gagcatttta taattttcat
      121 tgtgataaac tcgttattaa ctttaggata cactagttag gattaaatta ggtatctaga
      181 aatttactta agattttttt gtttatttcg tatacacact cgtatcaaaa acaatcgatt
      241 atgagacaaa atgaaatgac ctcttttatt aaataaaaga ggtcatattt tgtatcataa
      301 cttgttgcat taactatgta tctataactc ccttttatga tacaatcaag ccagaattaa
      361 agtacgaaag gaagaaaagc atgagtggat acatttatgg ttatgcccga gtgagtacac
      421 gttcacaaga actaaatcgt caattagact tactcaatga acaaaattgt aatgaaatcc
      481 taactgagaa aatgacggga accaaagcgg ttcgtcccca actaaatcgt ttaaaagata
      541 aaattcgtcc gggagatacg gtaattgtag aaagtttttc tcgattgggt cgtagtacaa
      601 aggatttgat tgaccttgtt acctattttg aagaacacca tgttaagctg gtgagcttga
      661 aagaaaactt cgataccagc acgcctcacg gacgcttaat gatgactgtc ttccaagcat
      721 ttagtcagtt cgaacgtgat ttaattgttg aacgtaccaa agaaggtttg aaaagtgcac
      781 gtgcgagagg tcgtaaaggt ggccgtccac gagtgaacca gaaagatgtt gatcgagcag
      841 taaaattgta taaaagtcag gtttatagcg taaaagaaat tacggaaatg actggtatta
      901 gcaaggcaac actctaccgc tacttaaaag acaataggga atagtctatt ttaatcttta
      961 ggaatctata atatttatgg gagaattaga gtgatttttt tattattact aaaaaacaat
     1021 ataaaataac gcattttaag ccctctttgt gcataaggat gaagctttta atttgaaaga
     1081 ggcttaaaat gcgttttaat tttttctatt tttcataaat atataggtta gtggtttaaa
     1141 attatagggt attacaactc tcagctgtta aaaattatct tttaaagtgt cgtctatcct
     1201 ctcaagttga aacattgggt tcttacctaa tcttttttaa ttgttcataa aaattttcat
     1261 gcgttccaca tagtaagata ggtatcactg cattatcttt aatttcatat gcaacgcgat
     1321 atgtcgtacc tgcatacttg aatccaatag cccaaattcc tgataaatca cccgtttttt
     1381 gttctcccga atgaggacga actgctatct catcataaat tatcgttaaa aattgttctt
     1441 ttaatgtttt ctcttttaat ttttttagat actttattac gtgtttagaa ggtagaatct
     1501 cataagccaa tttctgctgc caactcctct tttgtcatcg gtttagaatt tgcaacagta
     1561 tcttcagcaa tagatttaaa tgcagaagtt aacgcatttt ttctatttct aaattctttt
     1621 aagatctctt gtttgctata accttctgaa agaatatcag ccaaaatatc ttcactaaaa
     1681 tcaaaaaatt cttttttagg ctctacaaac tcataaagga taccattctc tacaagctta
     1741 gcttctactt caacaccatt gggcacatcg aattctttag ggaccgttaa cataatagaa
     1801 tttccttgtt ttcttgtttt tgtaatcata ttatttcctc cattatttta catttcgtaa
     1861 ttacttagta attacattgt acgctatttt tagtagaaat acaaaataat aaaactcctt
     1921 tagaacaatt ttcaaaggag ttttatttga ttaggaaatt atgtagaata ataatatgtt
     1981 tttaacttgg aatcaaaaac gcacttgcaa gtaagtttac ttcaatacat gaaattgctt
     2041 ataatagtgt attggaatta aggtagattg acaaaagagt gatcaaatag gtatacttca
     2101 ttcaaataat cgtttgaatg tgaggtgaga tgaatgaaaa aagtttgcca agtgactgtc
     2161 gtacataaag aaaaagtaaa tggaataaaa aataaattgg atcaaaaaga tttttcaaac
     2221 ttacttgtgt tagggaaatg tttcagcgat ttttctagaa ttaaaatttt ctatgcttta
     2281 gaaacctata aggaaatgtg cgtctgtgat ttagcagaaa tacttactgc tagtgtcgct
     2341 acaacgtcac accatttacg ttttttaaaa aaacatggaa tggcaaaatc acgtcaagat
     2401 ggaaaagtag tttattactc tttagcgaat gaagacatcc tttccgctat tcgtgttttt
     2461 taaagttatc agaaaatcta tctatgaaat cttagtttgg gggtaccgtc aggaaaatgc
     2521 ggatttacaa cgttaaggaa atttattaaa gagaccaatt cctcagcact aaaattgata
     2581 attgggcttt ctttgttact atagtgttag gtaagctttt tactttatca ccggttttcc
     2641 cctcacttca cacctacggg cgagtttccc cacatcccgt tttccatcta atccaattca
     2701 ttcaatatct ttatgtgtat gaaagatcag attgcttgat ttagacatct tacacgtaat
     2761 ttattcttaa atgggtacgg taaacgagca ataatacgag caaaagtata cctacaaaaa
     2821 taataactct gtctaatttt ttttgattca tatgcttatt gcttaaacca tataatttca
     2881 gtccaagaaa gcctccaaca gtatttgtaa ttacatctgt tatgtctgtc gctccaatag
     2941 cgaagataaa ttgaattatt tcaaaagtaa gacttaaaac cagtacaaaa gcaaacttag
     3001 gtaaaaatcc gatttcttta aaattgacat tcaaaagcaa gccaaatgga ataaagatta
     3061 taacattatc tatcatctct ctgaaattcc cagtagcagt aaatggagtc aagttaagac
     3121 ttctttgatg ataattaaat actgataaaa tattgtattg taatttgaat aacactaacc
     3181 agattagtgt cactaaatat aaagctagca atcctctaga taatattttt cccaatataa
     3241 tttcctctaa ttcttttgtt taagttgtat cattagtata gcaatgtcct agttaacgtg
     3301 ggttttaaat taaaaagcta acgttacccc aaaagtgcta gtacaataat gctgttcatt
     3361 aatttcgttg gtttcattcc gccatcctta cctccttgaa ttagtatgtg ttgatccggg
     3421 aaacactgtc acaattacac cgtctatatt gtttcctgat atctcataac gaagattagt
     3481 cggcacatga atggtggtat ttttagtaac aggataataa aagatctcat atttttcccc
     3541 atttacacta acagaaatgg ttttttcttc ttttaggtaa tccatatatt cctcgagaac
     3601 gaaattcttt tctttcataa tcgcactatg tggtaaacca acatagcgaa tatgccatag
     3661 ttcatattga attcctgtta actctgtttt gtcctctgga taacgtaaaa tgaacccgta
     3721 tttccaagca ttttcttcta tccactttcc ttcaggggct cgttccattt tcgtcaagct
     3781 tgatcctaca tctagtgata aacctgaatt atgctcacta taacctgctg gtaaggcata
     3841 ctcagccccc atttcttggt aaagcacact ttgctcatca aagtctcgat agccactatt
     3901 aataataaaa tgactaacgc caccctttac agcatcattg accatctctg aaaatttttg
     3961 tgctatttct tttgacatat aaatattact atcaagcaac ccgtatccat ttattaattc
     4021 gtcatgttta gataaattca cgatatctga cttcacactt tcttggcgaa caggatattt
     4081 actattgatt aatagcagat ttccttgata aacctgttct tctgtaattg ttttctcttg
     4141 ggtattttca gaagtcccac tattttctaa atgttctttg ggattttgat cataattttg
     4201 aaattcgact ttttcctgag aaaacagtgc ttcattaacg tagtcataac ctaagtatat
     4261 taagaataac aataacaata aaaaaaacaa cttcttcatt ttcagtctcc tccttaacta
     4321 tatagttgga agtatgaaat atttctagaa tcttcccctt aaacggcaca atctcgtttg
     4381 agattgtgcc gttttgcccc gtaaattaca gtcaattatt tcgccctact ggatttacta
     4441 ttatcaccaa tgtagatgaa ttttcataag tgatacccat gctgaattta acgaggctga
     4501 actgcctgtt gacaatgggc ggtgtgaaat accacccctc tatgcgatca gtcttattat
     4561 caggctatct tgccgcagcg cacgcagata atgaccgcaa tatcgctctg ccttacatct
     4621 tcaatactat ctactatata taaattccct aagaaccagt tacatacgtc gggtttcctg
     4681 ccgaaagagt tagcttatat agtttgtccg tgcaacggtt aaaagtttat ttaacgggga
     4741 aatcaaaata gctattgggg tatggttcgt ctcttaatac atagtgccac cattcgaggc
     4801 tatatgcttc aaacccactg ttttccatga tggagcgcaa acgtctgcga ttttgcgctt
     4861 cattgcatga tattccattt gccgcatgat gagagcgttc atccataaaa tcaaatcggc
     4921 tccccattgg tacaagctca cccgtgtcta atcgataaag cgtaagatca atggcactgc
     4981 cgcggctatg gcttgatttt gaagccacgt atccttttga aatcatctca gttcggtcaa
     5041 tattgggata ataactttcc tttgtcaggt tattttccgg ctgtgcagcc cattgcataa
     5101 aacagtttac agcacgatta ggacggtaac cgtcccatag aagcaatccg tacccttggg
     5161 tagcagccag ttcttttgcc ttcaaaagcg attcagccaa ctcgtatgtc cctacaatgc
     5221 gatttacttc ataaccgtca accggttttc cggtgaaatt atcccaagtg gcatatttag
     5281 cgtcccaacg aacaccgtgt actatttcat ctaaaaaagt aaatcctatt tccatgctta
     5341 tcaccccttt aacgctaata cgatcaagcg gtcaatcagt tcgggaagtg caatacctgc
     5401 agcggccatc atacggggat aacgactgta tgacgtgaaa ccgggcagag tattgacttc
     5461 gttcagtaca atgcggccgt tatcttgtaa aaacatatcc acacgggcta gacctctaca
     5521 gccgagcgct ttatatattt tttttgccgt ttcctgtatc cgtcctcgct cctctgctga
     5581 aaggtctgcg ggaacggtta taactgcgtt ttcagagcct ttttccggct cgacttcctg
     5641 atgaatacga aagattccgt actgcagcct gatttggtcc acctcgccaa cagctaacgc
     5701 ggcactgttt cccaataccg cacaaccgac ctcacagccc gaaacagcct gctcaattaa
     5761 gattttgctg tcatattgtc ttgccgattc aattgcgtag tccaattcgt ccgcgctatt
     5821 gacttttttc acaccgaagg atgagcctga acgcgccggc ttaacaaaaa caggataggt
     5881 aaacgtagct gccaccggcc tatcatcttt attaataacc caaaaggcgg gagtagctat
     5941 cccagcattt ttcgcaacga tgtatgtcaa cgatttgtcc atacaaattg ctgagctttg
     6001 aatatcgcag cctacaaaag ggataccgga caattcaaac agaccttgta tggatccatc
     6061 ttcacctgac ttgccatgca aagctgaaaa tgctacatca acatggttga tttcatattc
     6121 atggttcttt ttaacaagta atccgtgcat ttttttatcc ggcgagagta cagctgaata
     6181 gcaattgtcg ttttcccatt ccgcgcaagg tttttcgcac attttccata caccagattt
     6241 cgtaattcca atgtataacg gctcgtattt ttctttatta atgttagcgg ctatctctat
     6301 tgcagatttt accgatacgt catgctcctc tgagcaaccc ccaaacagta ttgcaacttt
     6361 tattctattc atgctcctgt ctcctttcaa aatccaaaca gtttttaatg gttttttcaa
     6421 cggtatcacg caacgcttgc tcggtataat aggccgtatg cggtgtgatt atcacgttag
     6481 gcattctttg aagtttaagt aaaaattgat tatcaattgg tttttgggtg caatcagagt
     6541 agaaaaactc ttcctctcct tccaatacat ccaatgcggc accgcccagt ttcccgtttt
     6601 ctaatgcttt aaccaactca taggtatcta caagtggacc gcgcccagta ttgataagaa
     6661 atgctccttg cttcattctc tgtatttgtt cgtggctgat aatatagtgc gtatccgtat
     6721 tgagcggcac atgaagcgta acgatatcgc tattttgcag caactcatca aacggtacat
     6781 agtttacctc tatacttcgg ctgcgactat aagccaacac tttacatcca aatcctcgca
     6841 gccgctcaat aaccgctttg cctatctggc ccgttcccac cacaccaact gtcatgtcgc
     6901 tgagtacctt gccacggtcg ctgtccaacc tgaaatcatg tttttccaca gagcgcacaa
     6961 tcgattttac gttgcgtact gccataagaa ttagcatcat agtataatcg gcaacgctat
     7021 ccggcgagta cgccacattg tcgacagtga tgcccattct cttagcagca gttgtatcta
     7081 tatgattgca gccgatgctt cgggtagaaa tatatttcac accggctctc ttcagcgcaa
     7141 gaagaataga ggcggaaatc tctgatttat gtcccacact gatacattga ttgaaaggcg
     7201 cggatttggc gttggattcc gacacgttgg cgttaattat cgttgccata acgccaaagc
     7261 gaggcgaaag agcatggaat gcatctgcct catcctgctc acatccataa acagtaatgc
     7321 cgatgttatt cataattaag accaaccctt tctgtgaaag gcaccaaata aaacggggat
     7381 gccaatggta atcttgtcaa atctttctct gaagaacgaa aacggctcgt tcttacggta
     7441 ttaagtctac cgtgtaagaa cgagccgttt cttaatatta aattgttaat ttcctaagaa
     7501 aaagtaaaga taaccttgag attttcctaa aaaat
//
LOCUS       DACCCR010000110         7530 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCCR010000110 DACCCR010000000
VERSION     DACCCR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.120"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            99..746
                     /locus_tag="IVK93_002528"
     CDS             99..746
                     /locus_tag="IVK93_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6387771.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IVK93_002529"
     CDS             1139..3784
                     /locus_tag="IVK93_002529"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP6387772.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IVK93_002530"
     CDS             4094..5407
                     /locus_tag="IVK93_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP6387773.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAELTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IVK93_002531"
     CDS             5397..6050
                     /locus_tag="IVK93_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6387774.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            complement(6115..7455)
                     /locus_tag="IVK93_002532"
     CDS             complement(6115..7455)
                     /locus_tag="IVK93_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6387775.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         2399 a         1315 c         1647 g         2169 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgagct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaaggctctt
     6061 cgtcaatgag tgttggtgaa gaaattcaat agaataatcg agatactcac acggctagac
     6121 agctatcttg tctagccgtg ttgttgttct ggctttagtc agttgggtag tgttcttagt
     6181 tttcttgaaa tattttcctc gatataaaaa gattcgttct cggtagtttt gaaagtttct
     6241 aaagccatag gcaattcgtt ttagtacttt gatatggttg ttcaaacatt ctatagggcc
     6301 gttggaatag ggaactcgca acgctcgttt gatttctgtt cgatatttct tgaaggtcgt
     6361 gaacactggt tgatagtgct ctggcaactc cttgtagtct tcttttagga gcgcgacgaa
     6421 ttcaggaaca tcttgtcttc tgatagcgga aagaaaaacc tggtagactt cgtagcctcg
     6481 ctttaaggag tcatcataat ataatagccg atcgactatc tccgcttctg tcagatgatc
     6541 tcgaaatgac ggacgccagt agcgatgctg accattcaat tcccacgcat tcttttgaag
     6601 tagtctccag tatttcttta agtgcttcga ccgacgatcc ccttttttaa aggtcttcat
     6661 tacttgaatt cggtggttgg taaaggcccg accgatgtgt tggctaatat gaaagcgatc
     6721 ggcgacgatt cgggcattcg gaaaacactc tttcaccaat gtgcgatagg gtgtatagaa
     6781 atctgaaacc actagacgta cttgttcacg gacttttcta gggtagcgca agaaatagtc
     6841 gcgtaaagga ttgagttgcc gattttccac aatatcaatc agttgatggg tcttgccgtc
     6901 catcatgaca aagctcatgg ctcccgagac gtttttcacc gatttgaatt catcgaaaca
     6961 caccacttct ggtaacggct ggttcaaaga aacggtttta ggccgaaggc tgcgaagtat
     7021 tcgaatgaca gtggtcggag aaatgtgctt catacgtgca atcaaagaca tagaaatagg
     7081 ctcacttaat agctcaagaa tggcttgttt gactctgcgg gcaatggagc agcgacgatc
     7141 gctaactgaa tcttctgcta aaaaagtctt tccacagtgt ttacatttga agcgctgttt
     7201 gttaatccgc acaatggtct tgtagttact gacatcattc aataagatgg atactttttt
     7261 ccagccccat ttaatgatcg tttgcttatt ttgatagtgg caatggaagc attcctctgg
     7321 ttgataggtc aatgtgccat caaaaacaaa acatgtttcg tcattaatgc gctctttgcg
     7381 aaaggcatct tcatgaaagg taagatttag gtctagaata tctagtaact ctttggtaaa
     7441 ctctgaatag gacatcttga aacactcctt tgaatggttt tcgtcgactt tattctaaag
     7501 gatttcaagg tgtctgtttg cgtttattag
//
LOCUS       DACCCR010000111         7235 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCCR010000111 DACCCR010000000
VERSION     DACCCR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.121"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(53..2041)
                     /gene="treP"
                     /locus_tag="IVK93_002533"
     CDS             complement(53..2041)
                     /gene="treP"
                     /locus_tag="IVK93_002533"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP6387776.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2264..2977
                     /gene="treR"
                     /locus_tag="IVK93_002534"
     CDS             2264..2977
                     /gene="treR"
                     /locus_tag="IVK93_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP6387777.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3054..3500
                     /gene="msrB"
                     /locus_tag="IVK93_002535"
     CDS             3054..3500
                     /gene="msrB"
                     /locus_tag="IVK93_002535"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP6387778.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3670..4272
                     /gene="ruvA"
                     /locus_tag="IVK93_002536"
     CDS             3670..4272
                     /gene="ruvA"
                     /locus_tag="IVK93_002536"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP6387779.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4285..5286
                     /gene="ruvB"
                     /locus_tag="IVK93_002537"
     CDS             4285..5286
                     /gene="ruvB"
                     /locus_tag="IVK93_002537"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP6387780.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5315..5893
                     /locus_tag="IVK93_002538"
     CDS             5315..5893
                     /locus_tag="IVK93_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6387781.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5945..6427
                     /locus_tag="IVK93_002539"
     CDS             5945..6427
                     /locus_tag="IVK93_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP6387782.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6544..6918
                     /locus_tag="IVK93_002540"
     CDS             6544..6918
                     /locus_tag="IVK93_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387783.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         2384 a         1323 c         1522 g         2006 t
ORIGIN      
        1 aaatccatga tagcccgttt tttttattga aaagattact tatctactct gactaagatt
       61 ttacttcagc tttaccgact gcttcgcccg ggcgaacaag tcctgattgg tcaagagaaa
      121 gtcctgcaac tttttcatta ttggtcaaag caacaataat gtcagttttc ttgccagctt
      181 gttctatttc tggcaaatcc atttcagcaa tcagggtctc tggtgtgaca aggtatcctt
      241 ctttcacgaa tacattgaaa ggaacaccct ttagttcaac agtatctaat cccatgtgaa
      301 caagtacttc cagcccctca ttagaaagaa taccaatggc atgttttgtt tcaaagacac
      361 tcgtcacttt accgtttact ggtgcataga ctttgccgtt tgacggttcg acagcataac
      421 cgtcacccat cattttttgt gagaaaactg ggtcatttac ttcagttatt tccttgatgg
      481 tccctgcagc gactgcgaat aatgtttctt ttgttgcaac tgccgtacca gttgctgaaa
      541 caggttcgaa agactgtggc gttgctgttg cgccatttgc tgtttgaagt tgtacatcag
      601 cagcattttc aggaacagca ggatcaatct tattcaaaat tccctgacgt ctaaagatga
      661 tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc gatgatgaaa ggtacccatg
      721 tttctgcttg gatagctagt attcctggca aaccgcctac tccgatcgcg ttagctcgaa
      781 cacccatcaa gttagcaaac atgccagcta atccagagcc gaccattgca gcgacgaaag
      841 gatagacgta tttcaaattg attccgaaca tagcaggttc agttacgcct aagtaacaag
      901 aaatcattgc tggaatcgag atttgttctt cttttttatt cccacgatgt aagaagatga
      961 tggctaagac tgcagagcct tgggcgatat ttgataaagc aatcatcggc cataaattcg
     1021 ttgatccgaa atctgcaatc agctgcatat cgatggcatt ggtcatatgg tggagaccag
     1081 taatcactaa aggagcatac aggaatccga atacggcact aaacaaccag ctgatagaag
     1141 acgtcaatcc agtatttacg atatttgaga tccagcttcc gactgtccag ccgataggac
     1201 ctaaaataac gtgtgcagct aaaacagttg gtaacaatga aaacaatgga acaaaaatca
     1261 tcgagatcga ctgagggata tatttgcgga agaagatttc tagatacgct aacataaacc
     1321 ctgccagcat ggctggaata acttgcgctt gataaccaat catctggact tgagcaaaac
     1381 caaaatccca aaatggaata tctgctgcgg ctgtggaagc tacactataa gcatttaata
     1441 attgcggaga aactaacgta attcctaaaa cgattcctaa tatttgtgta gtacccatct
     1501 tttttgcaat actccaagtg atacctactg gaagaaaatg gaaaattgct tcacccggta
     1561 accacaaaaa ggcattgaca ccattccaaa acttcgagtg ttcaacgatt gttccaccaa
     1621 gactttcaaa ctggatacct tcaagtacat ttctgaatcc aaggatcaat ccaccgacaa
     1681 tgattgcagg aatcagtggt gtaaagattt ctgctaaaac ggcaatagca cgttgaacgg
     1741 gatgaaggtt ttgttttgca gctgcttttc cttgttcttt ggaaacccct tcaactccag
     1801 atactgcaac gaagtcgtta taaaaagtag aaacatcgtt tcctatgatg acttggaact
     1861 gtcctgcatt cgtgaacatc ccttttacgc taggtatatc ttcgatcgct ttttcgtccg
     1921 cttttcctgg atcattcagg acaaaacgca tacgagtcgc acaatgacta actgcagcaa
     1981 tattttcttt tccgccgata tcatgtaaca gcttctcggc atctgcttga tattttgcca
     2041 tatcaataaa cccctttctc ttaacttgtc tatgcaagtt gtttcttcat ttactatact
     2101 ttttttcgga gagaattgca agcgttttat taaaaagtac gagagaaaat gatatcgttt
     2161 taaaaagctt aaaataagct tttcttaaaa ataggaacat tgaaatacaa aaagagctgt
     2221 agtatagtga agaaatgatg gaagaacgag tgagggaaaa aacatgaata agttcaatga
     2281 aattttttta gatttggaac agaaaatttt agcgaaagaa tatcctccgc attcgctttt
     2341 acccagtgag aaccaattga tcaaaattta tggtgtttca cgagaaacaa ttagaaaagc
     2401 gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa caaggaaaag gttcgatcgt
     2461 actggatcgg aatcgcttcg actttcctat ttctggattg actagctata aagagctaca
     2521 ggagacccaa cgtattccaa gtgaaacgat catccatacg ttagaagaaa cagaagtgac
     2581 aaaagcattg aacgagatca ctggctggga gatcggtgcg cctgtttggc atttgatacg
     2641 cgaaagaaaa atcaatggcg aagtagttat attagacacc gattatctat taaaggaaat
     2701 cgttcctatt ttaactccag cacaagcaca aggatcgatc tatgaatatt ttgaaaatga
     2761 gctttctttg acgattgctt acgcgcaaaa ggagatcaca gtagaagaag taacggaaat
     2821 catgaaaaca gcgatggatc ttcacgatga cacttatgca gtagtagtca gaagcctcgt
     2881 tcatctggaa gatacccgct gtttcgagta ttctgagtcg gttcaccgat tagataaatt
     2941 ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata gttcaaaaga aagcaaattg
     3001 ttggcatctg tccactttgg cgctatgata ggatcacaaa gggggcattt ttaatggata
     3061 aaccaataag accagaaaaa gaagaactga aagaaaagtt atcacccatg gcttatgcag
     3121 tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa atatgatgat ttttacgaaa
     3181 aggggatcta tgtagatatc gtcagcggtg aaccgttatt cagctctgca gaaaaatatg
     3241 atgcaggttg tggctggcca tcattcagca aaccaataac gaaaagacaa gtgcgcgaaa
     3301 aagccgattt ttctcatggc atgcatcgaa tagaagttcg aagcaaagaa gcagattctc
     3361 atctcggaca tgtgtttacc gatggaccag tagatcaagg tggtctacgt tactgcatca
     3421 attctgcctc actgaaattc attccatatg aaaagatgga tgaattaggt tatggggaat
     3481 tcaaatcact tgtagaataa tggaaataca caaagagcgg tacaatacgg ggatttactc
     3541 gatcgtccgc tcttttttta ttcaagcgat tgctatttga aagaaacgct ccctagactc
     3601 cttttttcac aaggtgaaat gtgctacaat aaagggaacg tatgtgtgca aaagaaagag
     3661 gactagaata tgtatgaata tctgacaggt gtggttacct ttatcaaccc ctattacata
     3721 gtagtagaaa ccaatggtat cggctaccag atcgcactag gaaatcctta ccgctacagt
     3781 agcaaactaa atcaaaaaat aaaaatttat gtccatcagg taatacgtga agatgcgcat
     3841 cttctgtatg gatttgattc gctagaagaa aagcaactgt ttttgcgatt agtaagtgtt
     3901 tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa gcgaagacca ctccggtttg
     3961 attcaagcag tagaatccag tgatgtcact tatttgacga aatttccagg agtagggaaa
     4021 aagacagcac aacaaatgat cttggatcta aaaggaaaat tcggtgagct gagtatcgat
     4081 acacctttta gtctatttga tgaagcaaac acaaaagacg ctacagctct atctgaagca
     4141 atggaagctc tctcggctct tggttacagt gatcgagaaa tcaaacgtgt agaaaaacaa
     4201 ttaaaagaag tagataatca gacaacggat gagtatctac gccaagcact caagctaatg
     4261 atgaaaaaat aaggaggaga ccaaatgaca gatgaatact tgttatcacc agaatctggc
     4321 gaaaatgaac tgagtttgga aaaatctctt cgaccacagc tgctttctca atatatcgga
     4381 cagagaaaag tcaaacaaga acttcagatt tacatcgaag ctgccagaaa tcgggaagaa
     4441 cctttagatc atgttctgtt gtacgggcct cctggattag gaaaaacgac gatggccatg
     4501 gtcattgcta atgagatgac cgtaaatatt cgtacgacga gtggccctgc tatcgaacgt
     4561 ccgggtgact tagtagcaat cttgaatgaa ctggaagcag gggatgtcct gtttatcgat
     4621 gagatccatc gattacctag agtggtagaa gagatgcttt attcagctat ggaagatttc
     4681 tatgtagata tcatggtagg acaagggcca accgcacatc cggttcattt ccctcttcct
     4741 ccctttactt tgattggcgc gacgacacgg gcaggtatgc tttctgcacc tttacgagat
     4801 cgatttggca tcatttctca tatggaatat tatgacgaac aggatctgca ggagatcgtt
     4861 ttacgttctt cggatatttt tcaaacagag atcattgaag aaggggcatt ggaaattgct
     4921 cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc tcaaacgggt tcgtgatttt
     4981 gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag cggatcaggc acttactttg
     5041 ctacaggtcg atcaggctgg attggattat gttgaccaga aattgttgcg aacaatgatc
     5101 gagttatatg gcggcggacc agtagggtta agtacgcttt cagtaaacat aggagaagag
     5161 cgtgagacgg tagaagatat gtatgaaccg tatctgatcc aaaaaggatt tttaaagcgt
     5221 acacctagag ggcgaattgc tactgcttat gcctatgaac attttggtta cgactatcat
     5281 atatgatttt tgcttttaga ttggaggaaa atgaatgagt gatacaacac gtacgaaaca
     5341 attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa tcttttcgaa aaatcagcgt
     5401 ccaaaaaatc gcaaacgaag caaaaatcaa tcgacaaaca ttttattatc attttacaga
     5461 taaatacgat ttgcttcgtt gggtctattg tgaggatgca ttgcattatt tagatccaca
     5521 atgtctttca cttgataatt gggaagagca agcattgaaa atgctgaaag caattgctga
     5581 gaagaaagaa ttttatgcaa atactgtgct ttctgaccca gaaattttac aaaaagagtt
     5641 ttctcaactg atccaaaaat catttatcaa gatttttgaa caaatggatg aagaaagaca
     5701 actttctgca agagacaaac tattttatgc tcgttttttt tcttacggtt gcagtggtgt
     5761 attagtcaat tggatcacga atgaatatga agaaacaccg atggaaatcg ctatgcagct
     5821 tttccgttta gccaaggata cggaattata ttcttatcgt ctttatgcaa aagaagagtt
     5881 ggaagaacag taatagaaaa attttttgtt ggaaaagcaa taatcgctaa aaggagatgg
     5941 ggaaatgaca aaaattttat ttgtttgttt gggaaatatt tgccgttcac cgatggcaga
     6001 aggcttgctg agaaaacgaa tagcattaga aggaagggaa gcagaatttt ttgtggattc
     6061 tgctgctaca agcacgtatg aagttggaaa gacaccgcac cctggaacga aaaaaatatt
     6121 aaaccaagaa aaagtagata tgacgaatat ggttgctcgg caaatcacac cccacgattt
     6181 tgaaactttt gattggatca ttggcatgga tcaagaaaat gtagaagaac tgaaacgaag
     6241 agcaccacgc tcagcgcagg ggaaaatcca tttgttttta agtagcattc ctggaaaaga
     6301 aaaagaaaat gttcccgacc catactatac caataacttt gatgagacat atcacttgat
     6361 cacagaaggc ctgaatcatt ggtggaatat ttggattgat gaaaataaaa taacaaataa
     6421 tttgtaaagt taacgctttc tttttaaggc gatgttagag catttccttg tttttttgta
     6481 aaatgttagg taaaataaaa ttaattaagt aacactttct tagctaaagg aggagtgcat
     6541 tcgatgaacg aagagacagt atcgccaatg aagaagctta cgaagttagt tactcatact
     6601 tcgtatcaac tttttgattc ttttgtaaat gacactgaag aacatgctaa gaatgtacga
     6661 ttgcaaggaa agtttattct tgagctgcaa aaagctgcta ctgaacatag tctagttatc
     6721 ttacaagtaa gagcaaatcg tttgagtagc aagtatgaaa cgatctcagg ctgggtggcg
     6781 acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac aaaacgaaga gcagcagatc
     6841 cggatggttc ctgtagaaca aattgaaaaa ataacaatcc tttcaccagc aggggaacag
     6901 gaacggttat caaaataatt tataccagat agaaaagcac gtatgagctt gtaccatcaa
     6961 tcattactaa tcaaaaataa ccgaatgctt taagctgata tcagtaaaac ggaacgtttt
     7021 aggatgagta tcatagcatg acagcattcg gttgattagt tgaatcgatt ggatggtaca
     7081 agtttttttg ataaaaaaag gtcctgctag ccggggagct agcaggataa ggagttaaaa
     7141 atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat ataatagtca ttagttgtga
     7201 atattttgtg acgaaagttt tcgaaagttg ttaag
//
LOCUS       DACCCR010000112         7024 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCCR010000112 DACCCR010000000
VERSION     DACCCR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.122"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            163..1104
                     /locus_tag="IVK93_002541"
     CDS             163..1104
                     /locus_tag="IVK93_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP6387784.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1173..1904
                     /locus_tag="IVK93_002542"
     CDS             1173..1904
                     /locus_tag="IVK93_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP6387785.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2048..4228
                     /locus_tag="IVK93_002543"
     CDS             2048..4228
                     /locus_tag="IVK93_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP6387786.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4248..4730
                     /locus_tag="IVK93_002544"
     CDS             4248..4730
                     /locus_tag="IVK93_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP6387787.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4746..5507
                     /locus_tag="IVK93_002545"
     CDS             4746..5507
                     /locus_tag="IVK93_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6387788.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5638..6972
                     /locus_tag="IVK93_002546"
     CDS             5638..6972
                     /locus_tag="IVK93_002546"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP6387789.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         2365 a         1229 c         1550 g         1880 t
ORIGIN      
        1 tttttctgtg cgaaacagga aagcgaagtc tttttaattc aaataataat attatgtaaa
       61 caaaaaactt tccatgcgta attatacaca agcaaataaa tcgtcaaatg cgaacgcctt
      121 ctttatactg tttaggaacg aaaagaagga aggaagaaat acatggatta tcaagtactg
      181 ctttattaca attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc
      241 ttttgcaaat ctttggatct taaaggtcgt attctagtag ctgaagaagg aataaatggt
      301 actgtgtctg gaaccgttga aaacacgact gcctatatga aagcaatgaa agctgatcct
      361 cgatttgctg atacgatgtt caagatcgat ccaagcgaag gacatgcctt taagaagatg
      421 tttgtaagat accgtccaga gcttgtaagt ttaaagctag aagaggacat tgatccaaac
      481 caagtgacag gtgcctatct ttcaccgcaa gaatttaaag aagctatttt agatgaacag
      541 acagtggtga ttgatgcacg gaatgattat gaatatgact tgggacattt tcgaggggct
      601 gtacgcccag atatacgcag ttttcgagag cttccagact ggatccgcga gcacaaagaa
      661 gaatttatgg aaaaacgggt agtgacctac tgtactggtg gcattcgttg cgagaagttt
      721 tctggttggc ttgtccgtga aggattcaaa gatgtcggac agctgcatgg cggtattgct
      781 acttatggta aggatcctga agtccaagga gaattatggg acggcaagat gtatgtgttt
      841 gatgaacgga tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg
      901 tttgacggaa caccatgtga acgatatatc aattgtagca atcctgagtg caatcgtcag
      961 attcttgctt ccattgaaaa tgaagaaaaa catttaggag catgtagcat agagtgtgcg
     1021 aaacatccgc gcaatcgcta tgttcaagac catggaatct ctccagaaga agcagaactg
     1081 cggtttacag ctttaattaa atagaaaaca atagttttga ttgtcgacca tttccaatat
     1141 gcgctatact agcgttgagg atggtgatcc acatgaaatc attgatttat gctattgggg
     1201 atgtccatgg caaacatgag ctttttgaaa aacttattca ctattatgat ccaaagatcc
     1261 atcagcttgt tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt
     1321 tattaggaaa gcaattagtc gaagaaacag gagccattta cctccgggga aaccatgaag
     1381 aatatttttt gcaatttctg caatcaccgg aggactggta tgcgccttat atgagaaatg
     1441 gtggaaaaga aacgtttgaa agcctgattc acccaggggc atcggaggaa tactccccaa
     1501 ctgaaatctc gatgatgatc caatcgagat attcagaact gattaaattt ttagtccagc
     1561 gccccttgta ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg gatttgacaa
     1621 agaaagactg gcgtaagaca agtctaagag actttatttg gataagggaa ccatttcatg
     1681 aagggaaaaa caatacaggt aagacaattg tatttggaca tacgattaca ccaatgctcc
     1741 atggagatat gcagacaaca gacctctgga ttcaggatca taaaatcgga atggacggtg
     1801 gggctgtatt cggagggtca ttgcatgggg ttgtttttga caaggaaggt attcttcaag
     1861 atattgaata tcagaacaac gaaggacctt ggcatggaat ctgactaatg gaaagaatga
     1921 aggagctgtg actttgttgc ggctcttttt atttttatag agaatattgt ttctttcagt
     1981 agtttaaccc aggataaaaa gtatgataga ataaagaagc aatttttagc agaaaagagg
     2041 cattttaatg acggaaaatt tgaaccagac gatcattcag cttgtacaaa aagaactttc
     2101 agactatcgt cctaagcagt tgactactgt acttaatttg ttaaatgaag gaaacactgt
     2161 tccttttatc gcgcgttacc gaaaagaaat gactggaagt cttgatgaag tccagattcg
     2221 tgaaatcgaa gagcggtatg cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat
     2281 tcggctaatc gatgaacaag ggaaactgac tcccgagtta gaagcagaaa tcacccaagc
     2341 cgtgaaaatg caacaagtgg aagatttgta tcgtccatac aaacaaaaac gacggacaaa
     2401 agcaacgatt gctaaagaaa aaggacttga accactcgcg aagtggctga tgcagcttac
     2461 agatggagag gtgcaatctg aagcagaaaa atatattgac aaagaaaaag aagtctcttc
     2521 tgccgaagaa gcgttgcacg gtgctcatga gattattgct gaacaagtca gcgataatgc
     2581 aaaatttcgg acttggattc ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt
     2641 caaggatgag caagcagatg aaaaaggcgt atatgaaatg tattatgact ttgcagaacc
     2701 cgtccataaa atggtttctc atcgaatatt agctactaat cgtggagaaa aagaagatat
     2761 tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat
     2821 agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta
     2881 taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc
     2941 agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc
     3001 actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc
     3061 agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc
     3121 agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca
     3181 agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc
     3241 tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc
     3301 ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga
     3361 aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat
     3421 cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc
     3481 tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac
     3541 agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat
     3601 tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc
     3661 acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa
     3721 aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt
     3781 aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac
     3841 acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga
     3901 tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt
     3961 attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac
     4021 ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact
     4081 tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt
     4141 aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt
     4201 gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag
     4261 aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc
     4321 aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc
     4381 atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca
     4441 tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag
     4501 atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga
     4561 cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc
     4621 gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat
     4681 ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa
     4741 ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt
     4801 tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt attttagctg
     4861 gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag
     4921 atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg
     4981 aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc
     5041 taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt
     5101 ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa
     5161 aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag
     5221 tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc
     5281 acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat
     5341 atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg
     5401 gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt
     5461 acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag
     5521 cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg
     5581 agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg
     5641 gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg
     5701 gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc
     5761 tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat
     5821 gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc
     5881 cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt
     5941 atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga
     6001 ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca
     6061 ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat
     6121 ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat
     6181 tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa
     6241 aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg
     6301 atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca
     6361 acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta
     6421 ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc
     6481 gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt
     6541 cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct
     6601 gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt
     6661 gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa
     6721 gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa
     6781 caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc
     6841 aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt
     6901 gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa
     6961 aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca
     7021 aaaa
//
LOCUS       DACCCR010000113         6922 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCCR010000113 DACCCR010000000
VERSION     DACCCR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.123"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(181..645)
                     /locus_tag="IVK93_002547"
     CDS             complement(181..645)
                     /locus_tag="IVK93_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6387790.1"
                     /translation="MFKMFFNKKKEDIFAPVNGQIIKLENVNDPVFSSKAMGDGFGIE
                     PEENHIYSPIIGKVISVFPTKHAILLETKKGQSVLVHIGIDTVELNGQGIDVHVAEGQ
                     KVSEETLLATVDLPFILEQGKQTTVIVAFEDIASIELINNNHVKVHEKLGDL"
     gene            complement(638..2758)
                     /locus_tag="IVK93_002548"
     CDS             complement(638..2758)
                     /locus_tag="IVK93_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAP6387791.1"
                     /translation="MEIVENNYLVHRYWGKKVDSYHFSNRPILKKRSFAAAPTIEKPE
                     ISPEFLPLEISFPHQGDYRSAAVQIQQANGEEITRLCYKNYEIRNEAVELTDLPHVRN
                     TDEPAQTLIIFLYDQASKIELALYYTIFENSPMIVRSSSVKNDGIHSIKISKLLSASI
                     DTRYDQQFSTTFYGTHQKEFQLNRSEIQHGLFKIGSSRGASSPQYPPFIALSKDANEF
                     QGEVHAMTVIYSGNHEITLERDQYNQIRMQIGINSETFSWRLEPGETFYSPQAILSYS
                     DEGFNGSSQNFHHFFSDHLIHPAWRNRERPILINSWEMTYYDVNDKLITDLIDAANDL
                     GFETVVLDDGWYGKRNSSKTSLGDWQVDTDKFPNGIESLVDYAKQKNIGFGIWFEPEM
                     ISPNSELIQKHPDWVMRSKKYEPLLSRSQYVLDLTKQEVQQFIIKTLSETITKLRVNY
                     IKWDMNRHISDPFSQDSTYEPSEYSHRYMLSLYNIINKLTNAFPDVLFENCSSGGGRL
                     DPGMLYYFPQTWISDNTDGLDRQQIQYGASHLFPVSSMTGHVSDVPNHQTNRSVSFET
                     RAALASSTNMGYEMDIIHLTEDEKESVRNHLKEYKRERTLIMEGEFYRLFSPFDGNIC
                     AWLFTNKEQILIYVFRNTYKVFDLSLLVKIPTLDSNALYRDETTNQVYSGSELSNCGL
                     ALENTKGDFIVKRIKLDKLETERV"
     gene            complement(2833..3687)
                     /locus_tag="IVK93_002549"
     CDS             complement(2833..3687)
                     /locus_tag="IVK93_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6387792.1"
                     /translation="MYNKFMNNNLREYTDKFTDFSKKSISDFILYNSGIEYCEPNYSY
                     GPKRRDYHFIHFVKEGKGLLEIEDQKIEVHENQIFIVPANIVSTYTADKQSPWKYSWI
                     GFTGIESVNFVQTLIQSSPANFVMDCSDASFYEKRIEEIIELNQNTLASYFKINGIMY
                     DIVGTLLGECEIKTVKNLETSPSFQAMRYMELHYHDDIQISDIAYSVGIHPSYLSAMF
                     KEEIGVSPKKYLTNLKINKAKKLLTESDDPINLIAGSVGFADALSFSKFFRKELGLSP
                     TEFRKDHN"
     gene            3841..5181
                     /locus_tag="IVK93_002550"
     CDS             3841..5181
                     /locus_tag="IVK93_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP6387793.1"
                     /translation="MENQHKNSFHIEPAKGLLNDPNGLIQFKGMYYFFHQWNRFETNH
                     NYKEWGLFTSKDLVKWESQGSAILPDLEDDKGGIYSGSAIEKDGKMYLFYTGNSKKHG
                     KRKSYQKIAYSEDGRTFIKKDQVIETPIGYTEHHRDPKVWEKNGVWWMIVGAQTQANG
                     GAITLFESSDLVNWQFNGEFYSDEKLDQMCECPDVFSLSDDVDILMVCPQKRTSIEET
                     DLSLSSYAGYLVGKIDYLQKKFIEKEELRKLDHGFDFYAPQTFKDDKDRLIMTAWMSR
                     MDDEEEANCPTLIDGYLHCLTMPRELKWKNNHLYQVPLEEYQVLRKDQQEFKNKSHLI
                     KNQNSAYEMIIEFNESSSDFSLSLNSEMNAINYSDGFLEISRINWVSGKSEAKRIELS
                     SLFKLQIFNDTSAMEVFINDGEYVFSMRTFSNNLKRDVYYQNLSHNGSVMFYNY"
     gene            5194..6579
                     /locus_tag="IVK93_002551"
     CDS             5194..6579
                     /locus_tag="IVK93_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6387794.1"
                     /translation="MNNKDIAKKILELVGGNENVQSVTHCATRLRIITYDKDKIDIKA
                     VEDIDKVKGSFFNSGQYQVILGTGLVNKVYEEFVPLIDGSGKDIAEEPAEKQKMTFQR
                     AIRIFGDVFVPIIPVLVATGLFMGLRGLFTQEAVLGVVGLSPDDIPQQFLMITQILTD
                     TAFAFLPALVAWSTFRIFGGTPILGIVLGLMLVSSVLPNAYEVGQQAAEPLVFFGFIK
                     VVGYQGSVLPAFVTGILGSKIEIFLRKRIPDSLDLILTPFLTLLTGLILALFIIGPIF
                     HEVEIGVLHAVEFLLNLPLGIGGLIYGSFGQLLGIFGIHHILNFLEISMLAQDGWNYL
                     NPIGTCGNIAQAGAVLAVAIKSVSPKMKQIAYPSALSAALGITEPAVFGVTLRLIKPF
                     IMSMIGGGVGGFLASLLHLRATGMALTGIPGTLLYLNEQLPLYILVNLVAFAVSFGLT
                     WMIGYKKDQEL"
     gene            complement(6819..>6922)
                     /locus_tag="IVK93_002552"
     CDS             complement(6819..>6922)
                     /locus_tag="IVK93_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6387795.1"
                     /translation="WTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT         2102 a         1218 c         1359 g         2243 t
ORIGIN      
        1 aacaaatgag tacaacaaat agacctttca ctattagatt tttttgattg ttcaatagta
       61 tttaaaggaa aaagaactta cacaaaattc tttacggacc ctcaaactct ttggtagctc
      121 atttttagat atcgttcggg ttgtgcttta taagacataa gtaacttttc tcctttgaac
      181 ctatagatct cctaattttt catggacttt tacgtggtta ttattgatca actcgattga
      241 tgcaatatcc tcaaatgcaa cgatgacagt cgtctgtttt ccttgttcta agataaacgg
      301 aaggtcaact gttgccagca atgtctcttc gctaactttt tgcccctcag caacatggac
      361 atcgattcct tgaccgttta attcaactgt atcaatccca atatggacta gaacagactg
      421 tccttttttt gtttccaata aaatcgcatg tttggtcgga aatacactaa tcactttgcc
      481 gatgattggg ctgtagatgt gattttcttc aggttcaatc ccaaatccat ctcccatcgc
      541 tttagaagaa aaaacaggat cattgacgtt ttctagtttg attatctgac cgttgaccgg
      601 cgcaaaaata tcttcttttt tcttgttgaa aaacatctta aacacgctcc gtttctaatt
      661 tatctagttt tattcttttg acgatgaaat cgcctttagt attttccaaa gccaatccgc
      721 aattagataa ctctgatccc gaatatactt gattagtggt ttcgtctcgg taaagggcat
      781 tactgtccag agtaggtatc ttgaccaata ggcttagatc gaatacttta tacgtattgc
      841 ggaagacata aataaggatt tgttctttat tcgtgaacag ccatgcacaa atatttccat
      901 caaaaggaga gaacagtcgg taaaattctc cctccataat aagtgttctt tcacgtttgt
      961 attctttcaa atggttccga acagattctt tttcatcctc agtcaaatga ataatatcca
     1021 tctcatagcc catattggtg gaagacgcga gagctgctct cgtttcaaag gaaacgctcc
     1081 gatttgtttg gtggttcggc acatcggaaa catgaccggt cattgatgaa acagggaata
     1141 ggtgactagc tccatattga atttgctgtc gatccagacc atctgtgtta tcgctgatcc
     1201 atgtttgcgg aaaataatag agcattcctg ggtctaatct accaccgccg ctggaacaat
     1261 tttcaaaaag tacatccgga aaagcgttcg ttaacttatt gatgatgttg tacagactta
     1321 gcatgtaacg atgactgtac tctgaaggct catacgttga atcctgagaa aaagggtcag
     1381 atatatggcg attcatgtcc catttaatat aattcacccg aagttttgtg atagtctctg
     1441 ataaggtttt gataatgaat tgttggacct cttgctttgt aagatctagt acatattgac
     1501 ttctgcttag taacggctca tattttttgc ttctcatgac ccaatcagga tgcttttgga
     1561 tcagttcaga attaggagaa atcatttcgg gttcaaacca gatgccaaac ccaatatttt
     1621 tctgctttgc ataatcaact aatgattcga tgccattagg aaatttatct gtatctacct
     1681 gccaatcccc caaggacgtt ttactactgt ttctctttcc ataccaacca tcgtctaata
     1741 ccactgtttc aaaccccaaa tcattcgcag catcaattaa atcagtgatc aacttgtcat
     1801 ttacatcgta ataggtcatt tcccaactat taatcagaat agggcgttct ctatttctcc
     1861 atgcaggatg gatcaaatga tcagaaaaaa aatgatggaa attctgcgag ctgccattga
     1921 agccttcatc actgtaagac aagatggctt gaggactata aaaagtttca ccaggttcta
     1981 atctccatga aaaagtctcg ctatttattc cgatttgcat tcgtatttga ttatattggt
     2041 ctctttctag ggttatttcg tgatttccac tgtaaatcac tgtcatcgca tggacttcgc
     2101 cttgaaattc gtttgcatcc ttggataaag cgatgaaagg aggatattgc ggactgcttg
     2161 ctcctcttga gcttccaatc ttgaataaac catgctgaat ctcacttcga tttaactgaa
     2221 attctttttg atgggttcca taaaaagtcg tcgaaaactg ttgatcatat ctggtatcaa
     2281 ttgaagcgct caataattta gaaatcttta tcgaatggat accatcattt tttacgcttg
     2341 aggagcggac gatcatagga gaattttcaa aaatcgtata gtacaaagcc agttcgattt
     2401 tcgaagcctg atcgtataga aagataatca acgtttgtgc tggctcatct gtgtttctaa
     2461 catgaggcaa atctgttagc tccacggctt catttctgat ttcatagttc ttatagcata
     2521 acctggttat ctcttctccg tttgcttgtt gaatttgtac agcagcactt cggtaatctc
     2581 cttgatgagg gaatgatatt tctaatggca gaaattcggg agaaatttca ggcttttcga
     2641 tagtaggtgc agctgcaaag gatcgctttt ttaaaattgg tcgattactg aagtgataac
     2701 tatcgacctt tttaccccaa taacggtgaa caagataatt gttttccacg atttccataa
     2761 tataggtaac gtgattgttt gatagtgtga aggtgttttt ttctgagtca tagtgaatga
     2821 gttctcgcat gattagttgt gatcctttct aaattcagta ggggagagtc ccagttcttt
     2881 tctaaaaaat tttgagaagg aaagggcgtc ggcaaaacca accgatcctg caatcaagtt
     2941 gattggatcg tcgctttccg ttaatagttt tttggcttta ttgattttta aattagtcaa
     3001 atacttctta ggcgaaacgc cgatttcttc tttaaacatg gcggacaaat aacttggatg
     3061 gatacctaca gaataggcaa tatcagaaat ttgaatatca tcatgatagt gcagctccat
     3121 atatcgcatt gcttgaaaag agggggaggt ttcaagattt ttcactgttt ttatctcgca
     3181 ttctcctaaa agggttccta cgatgtcata cataatgcca ttgatcttaa agtaagaagc
     3241 cagtgtattt tgatttagct caatgatctc ttcaattctc ttttcataga aggaagcatc
     3301 cgaacagtcc ataacaaaat tagctggact ggattgaatc aaggtctgaa caaagttaac
     3361 agattcaata ccggtgaaac caatccaaga atacttccaa ggactttgct tatcagctgt
     3421 ataggtagaa acgatattgg ccggtacgat aaagatttgg ttctcatgga cttcaatttt
     3481 ctgatcttca atttccaaca gacctttgcc ctcctttaca aagtgaataa agtgatagtc
     3541 tcttcgtttc ggaccatagc tataattagg ttcacagtat tcgattccgc tgttgtataa
     3601 aatgaagtct gagatactct ttttcgaaaa gtcagtaaac ttatctgtat attctcttag
     3661 attattattc ataaatttat tatacatggt aacgtttaac aaaggatgtc tatcttagaa
     3721 atttccatat atccattaat tttttccata tttcaaatct attaaaggat gttaagatct
     3781 cattaaccaa ggatatgaaa acgcattaat gcaatttatt aaaaggaggt ataagggaat
     3841 ttggagaatc agcataagaa ttctttccat atagagccgg caaaaggatt attaaatgat
     3901 ccaaatggat taatccaatt taaagggatg tactattttt ttcatcagtg gaatcgcttc
     3961 gaaacgaatc ataattataa agagtgggga ctatttactt ccaaagattt agtgaaatgg
     4021 gagagtcaag gatcagccat tttaccagat ttagaagacg ataaaggtgg tatctattca
     4081 ggcagcgcga ttgaaaaaga tggaaaaatg tatcttttct atacaggaaa tagtaaaaag
     4141 catggaaaaa gaaaaagtta tcagaaaatt gcctactctg aagatggaag aacttttatt
     4201 aagaaggatc aagtcatcga gacacctatt ggttataccg agcatcatcg cgatcccaag
     4261 gtttgggaaa aaaatggggt gtggtggatg atcgtaggtg cacagacgca agccaatggt
     4321 ggagccatca cattatttga atcaagtgat ctagtcaatt ggcagtttaa tggtgagttt
     4381 tattcagatg aaaaattgga tcagatgtgt gagtgtccgg atgtattttc tttgtctgat
     4441 gatgtagata tcttaatggt atgtccacaa aaaagaacct ctattgaaga aacggattta
     4501 tcgctttcta gttatgcggg gtatttagtt gggaaaatcg actatctgca aaagaagttt
     4561 atcgaaaaag aagagctgag aaagttagat catggttttg atttttatgc cccacaaacg
     4621 tttaaagatg ataaggatcg gcttattatg actgcttgga tgtctcggat ggatgacgag
     4681 gaagaagcca attgtcctac gcttatagat ggttacttac attgtctgac gatgcctaga
     4741 gagctgaaat ggaaaaataa tcatctttac caagtcccgc tggaagaata tcaagtatta
     4801 agaaaagatc agcaagaatt taaaaataaa agtcatttaa tcaaaaatca aaatagcgca
     4861 tatgaaatga ttattgaatt taatgaatca agctctgact tttcattgag tttaaactcg
     4921 gaaatgaatg cgatcaacta tagtgacggt ttcttagaga ttagtcgaat caattgggtt
     4981 tcagggaaaa gcgaagccaa gagaattgaa ctgagttcac tattcaaact gcaaattttt
     5041 aatgatactt cagcaatgga agtgttcatc aatgatggcg agtatgtttt ttcaatgaga
     5101 accttcagca ataatctcaa gagagacgta tattatcaga atttgagtca caacggttcg
     5161 gttatgtttt ataattatta aggaggataa caaatgaata acaaagatat tgcgaagaaa
     5221 atcttggaac tagttggcgg aaatgaaaat gttcaatcag tgacgcattg tgcgaccaga
     5281 cttagaatca ttacgtacga taaggacaaa attgatatca aggctgtcga agacattgat
     5341 aaagtaaaag ggagcttctt taactcagga caatatcaag tcatattagg aacagggtta
     5401 gtgaataaag tgtacgaaga atttgttcca cttatcgacg gttcaggaaa agatatcgct
     5461 gaggagccgg cagaaaagca aaaaatgact tttcaacggg cgatcagaat atttggggat
     5521 gtgtttgttc cgattatccc agttttagtc gctacggggc tttttatggg gttgcgagga
     5581 ctatttacac aagaagccgt cttaggtgtt gtcggtcttt ctccagatga cattccccaa
     5641 caatttctga tgatcacgca aattctaaca gatacggctt ttgcctttct gcctgcttta
     5701 gtcgcttggt ctacttttag aatttttggc ggaacaccta ttttggggat tgtattaggg
     5761 ctaatgctcg taagttcagt attaccgaat gcttatgagg taggacagca agcagctgag
     5821 ccactagtat tttttgggtt tattaaggta gttggttatc aaggttcggt attgccagcc
     5881 tttgtaacag ggattttagg ttcaaaaatt gaaatattct tgcgaaaacg cattccagat
     5941 tcactagatt taattttgac gccattcttg acattattaa caggacttat tttggcatta
     6001 ttcattattg gaccgatttt ccatgaagtg gaaattggcg ttttgcacgc agtagaattt
     6061 ctattaaatc ttcctcttgg aatcggagga ttgatctatg gtagttttgg acaattattg
     6121 ggtatctttg gtattcatca tatcttgaat ttcttagaaa tcagcatgtt agcgcaagat
     6181 ggttggaatt acctaaaccc aattggaact tgtggaaata tcgcacaagc gggagcagtt
     6241 ttagcagtgg caatcaaatc agtttcacca aaaatgaaac aaattgccta cccctcagca
     6301 ctttccgctg ccctaggaat tactgaacct gctgtttttg gtgtaacatt acgtcttatc
     6361 aaaccattta tcatgtcaat gattggtggc ggagtaggag gcttcttagc atcgttgcta
     6421 cacttaagag caaccggaat ggctttgaca ggtatacctg gaacattgct ctatctgaat
     6481 gaacaattgc cattgtatat cttagtcaat ctagtcgcat ttgccgtttc ctttggatta
     6541 acttggatga ttggatataa aaaggatcaa gaattgtaag ataatttttt taaatcgtat
     6601 attagagaat tgcatttaag tattagactt taaaaggaca cttcttaatg acattctagt
     6661 gaactactca ccacttagct aaacctaacg gtttcttacg cttgaagtgg gagcttcttg
     6721 ggaagagagc atacttgata gcgtattact ttactaacag aggtgcctct tatttaccaa
     6781 gctatccccg tagttcctac ggttcttgtt tatttgatct aagccaatcc catagagtgt
     6841 agccttagac cttcggtcag gatattgata ctagcgttga tatctcggtc atgatgagta
     6901 tgacaaatag ggcaagtcca tt
//
LOCUS       DACCCR010000114         6902 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCCR010000114 DACCCR010000000
VERSION     DACCCR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.124"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            126..461
                     /locus_tag="IVK93_002553"
                     /pseudo
     CDS             126..461
                     /locus_tag="IVK93_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            626..853
                     /locus_tag="IVK93_002554"
                     /pseudo
     CDS             626..853
                     /locus_tag="IVK93_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            979..1950
                     /locus_tag="IVK93_002555"
     CDS             979..1950
                     /locus_tag="IVK93_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6387796.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2090..3403
                     /locus_tag="IVK93_002556"
     CDS             2090..3403
                     /locus_tag="IVK93_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6387797.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3446..4312
                     /locus_tag="IVK93_002557"
     CDS             3446..4312
                     /locus_tag="IVK93_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6387798.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4309..5157
                     /locus_tag="IVK93_002558"
     CDS             4309..5157
                     /locus_tag="IVK93_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6387799.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5171..6643
                     /locus_tag="IVK93_002559"
     CDS             5171..6643
                     /locus_tag="IVK93_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP6387800.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6660..>6902
                     /locus_tag="IVK93_002560"
     CDS             6660..>6902
                     /locus_tag="IVK93_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6387801.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2165 a         1169 c         1471 g         2097 t
ORIGIN      
        1 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
       61 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
      121 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
      181 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
      241 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
      301 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
      361 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
      421 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
      481 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
      541 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
      601 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
      661 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
      721 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
      781 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
      841 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
      901 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
      961 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     1021 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     1081 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     1141 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     1201 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     1261 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     1321 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     1381 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     1441 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     1501 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     1561 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     1621 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     1681 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     1741 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     1801 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     1861 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     1921 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     1981 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     2041 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     2101 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     2161 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     2221 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     2281 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     2341 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     2401 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     2461 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     2521 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     2581 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     2641 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     2701 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     2761 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     2821 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     2881 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     2941 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     3001 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     3061 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     3121 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     3181 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaaatttc
     3241 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     3301 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     3361 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     3421 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     3481 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     3541 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     3601 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     3661 tgttgtttgg attattccgg tccaattagg ggcagcttta ggaatggcac ttattatcaa
     3721 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     3781 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     3841 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     3901 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     3961 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     4021 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     4081 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     4141 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     4201 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     4261 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     4321 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     4381 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     4441 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     4501 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     4561 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     4621 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     4681 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     4741 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     4801 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     4861 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     4921 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     4981 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     5041 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     5101 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     5161 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     5221 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     5281 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     5341 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     5401 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     5461 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     5521 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     5581 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     5641 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     5701 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     5761 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     5821 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     5881 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     5941 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     6001 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     6061 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     6121 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     6181 ttatcaagta attgatccgc agaaaaggat tgaatgggcg aagactgaag agaatcgctt
     6241 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     6301 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     6361 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     6421 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     6481 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     6541 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     6601 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     6661 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     6721 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     6781 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     6841 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     6901 aa
//
LOCUS       DACCCR010000115         6658 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCCR010000115 DACCCR010000000
VERSION     DACCCR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.125"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(58..354)
                     /locus_tag="IVK93_002561"
     CDS             complement(58..354)
                     /locus_tag="IVK93_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP6387802.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            709..1065
                     /locus_tag="IVK93_002562"
     CDS             709..1065
                     /locus_tag="IVK93_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6387803.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1075..1974
                     /locus_tag="IVK93_002563"
     CDS             1075..1974
                     /locus_tag="IVK93_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP6387804.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2491..3222
                     /locus_tag="IVK93_002564"
     CDS             2491..3222
                     /locus_tag="IVK93_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6387805.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3367..5394
                     /locus_tag="IVK93_002565"
     CDS             3367..5394
                     /locus_tag="IVK93_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP6387806.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5502..6599
                     /locus_tag="IVK93_002566"
     CDS             5502..6599
                     /locus_tag="IVK93_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP6387807.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         2280 a         1088 c         1344 g         1946 t
ORIGIN      
        1 atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa cctctattta
       61 ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat aaaaatcggc
      121 gggaagctta ctccaccgtt ttaataattg atagaaataa ctctcttctg ctttgatttt
      181 atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt ttttataaat
      241 atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa tataagaacc
      301 ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct tcatagtgat
      361 tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt taataatccg
      421 tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt ttatagtatt
      481 ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt tgggtctttt
      541 tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat aagagaaatc
      601 ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca gacaaattag
      661 ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat gaaattagaa
      721 gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga tcaactttta
      781 ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg ccaattggat
      841 acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag tctttattct
      901 gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat actttacatg
      961 gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt aaaaggacca
     1021 agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga taaaatggga
     1081 aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga atcagagctg
     1141 atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct agccggagat
     1201 acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta
     1261 tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga acagatgatg
     1321 gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa tgagcagacc
     1381 gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa agattacgag
     1441 gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg tgggaaaaca
     1501 gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga ccagctggaa
     1561 gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc atttatcatg
     1621 tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg atcagaatca
     1681 ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg ccatacgcat
     1741 cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc ttttggttat
     1801 tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct agacagctat
     1861 gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca ggcctatcaa
     1921 acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta ttgagacagc
     1981 attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag agcagtagat
     2041 ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg tttatttgat
     2101 cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt ttgaacgata
     2161 atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat tgattgaagt
     2221 ctaggcagtc aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc
     2281 tatgacagct gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa
     2341 atcgggctga taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc
     2401 ttttatttca taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata
     2461 tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca
     2521 gcaatgatgt tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac
     2581 ccaaaggaat ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact
     2641 cctcctgttg atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca
     2701 agccctggaa caggaggggc attatcgatt gattatggat caaaattcaa atttgggact
     2761 caaaaaattt ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg
     2821 tctagaaaac cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa
     2881 ttaagtgtct ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca
     2941 cagctgaaat ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa
     3001 acggtgaatt ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct
     3061 aaatcaggaa caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat
     3121 caagatgcgg tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc
     3181 actaaattag tttggacatt agaagataca cctaacaact agtttactga aaagaactgt
     3241 taaaaataaa tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc
     3301 taggctaaat ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa
     3361 gatatattga aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc
     3421 tttccgatca tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac
     3481 gaagaaagca aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa
     3541 agcagtgagg atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag
     3601 ggttcggaca aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat
     3661 cccaatatta tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata
     3721 ttggaaaatg ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt
     3781 tggtcaaata agaagattag tttactctcg gattttacat ttaaatctta tttatactta
     3841 ggaaatcaac gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga
     3901 atggctagta atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct
     3961 actcgttctg gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa
     4021 aatagtggtt cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga
     4081 catgtcgctt ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc
     4141 gtgacggtag ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg
     4201 gacgcggagc aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt
     4261 atacctgatc tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca
     4321 acaggtactt tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca
     4381 tcaacagctg atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga
     4441 aacgataaga taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag
     4501 aaaatgcaga ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc
     4561 aatggaaatc aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat
     4621 ctgacagatt attttattgc tggagatacg ttaataatcg aattggagac aactttgcag
     4681 gaagtaattc ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg
     4741 attgagcaat cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat
     4801 gtttattata aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg
     4861 aaaataggag agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg
     4921 gattcagcga atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac
     4981 agaaaaggca aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa
     5041 atatccagta gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat
     5101 gaacgggctt ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga
     5161 gattttgagc ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg
     5221 acatccgctg ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt
     5281 ttagattcta cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc
     5341 ggaacctttg aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa
     5401 gagaagaata taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt
     5461 gtcccacaga gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt
     5521 tcttgagacc aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta
     5581 acaatatatc agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga
     5641 atcaaatcga caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag
     5701 aagtaaaagc ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga
     5761 atagcgcaac caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag
     5821 acatgagcct gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa
     5881 aacctaaaag tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg
     5941 gtgtattggc tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa
     6001 ctaaggatca aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc
     6061 aacagacact gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa
     6121 tcaatgcaag aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc
     6181 aagtagttat tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg
     6241 aaaaagaagg attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg
     6301 gtcaaccgct aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg
     6361 aaaatggaaa attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg
     6421 tatttgaaaa agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg
     6481 ttacgattaa aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt
     6541 ttattttatt atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag
     6601 gttaagataa caaatagtaa atttttatta ttgtaggatg gttgggctct ttttataa
//
LOCUS       DACCCR010000116         6065 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCCR010000116 DACCCR010000000
VERSION     DACCCR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.126"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            832..1566
                     /locus_tag="IVK93_002567"
     CDS             832..1566
                     /locus_tag="IVK93_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6387808.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IVK93_002568"
     CDS             1582..2751
                     /locus_tag="IVK93_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6387809.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IVK93_002569"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IVK93_002569"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP6387810.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="IVK93_002570"
     CDS             3841..4767
                     /locus_tag="IVK93_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP6387811.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="IVK93_002571"
     CDS             4786..6036
                     /locus_tag="IVK93_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6387812.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2025 a          997 c         1246 g         1797 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agtta
//
LOCUS       DACCCR010000117         5965 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCCR010000117 DACCCR010000000
VERSION     DACCCR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.127"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVK93_002572"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVK93_002572"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP6387813.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IVK93_002573"
     CDS             complement(1098..2201)
                     /locus_tag="IVK93_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP6387814.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IVK93_002574"
     CDS             complement(2291..3226)
                     /locus_tag="IVK93_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP6387815.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IVK93_002575"
     CDS             3528..5462
                     /locus_tag="IVK93_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6387816.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT         1750 a         1168 c         1180 g         1867 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tggga
//
LOCUS       DACCCR010000118         5925 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCCR010000118 DACCCR010000000
VERSION     DACCCR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.128"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(76..1170)
                     /locus_tag="IVK93_002576"
     CDS             complement(76..1170)
                     /locus_tag="IVK93_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6387817.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IVK93_002577"
     CDS             1359..2120
                     /locus_tag="IVK93_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6387818.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IVK93_002578"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IVK93_002578"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6387819.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IVK93_002579"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IVK93_002579"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP6387820.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IVK93_002580"
                     /pseudo
     CDS             complement(3760..4773)
                     /locus_tag="IVK93_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
     gene            4938..5858
                     /locus_tag="IVK93_002581"
     CDS             4938..5858
                     /locus_tag="IVK93_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP6387821.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1807 a         1181 c         1062 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattaatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DACCCR010000119         5759 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCCR010000119 DACCCR010000000
VERSION     DACCCR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.129"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1579
                     /locus_tag="IVK93_002582"
     CDS             <1..1579
                     /locus_tag="IVK93_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP6387822.1"
                     /translation="FMVLPEYTEIADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQI
                     QTLVDNDIKIVGRYLTGTVGIGKDERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAY
                     FTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSY
                     SGYQTGVYGTRNVCSQIINNGYASFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIG
                     SGNGAVGIDLIATSGRDSGFNELSNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDN
                     PWNPHLTFYRYVNYSGLSWDIISSPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGS
                     IIGLPHLIVTLQSQMFLADTINDSVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVG
                     TAAGGTFSLEDLYQDVDAVNVYTLAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRF
                     GSSDKIASVTNSLLENDETVDILGVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVAN
                     GFSEKIIQFQENRSKLN"
     gene            1659..2054
                     /locus_tag="IVK93_002583"
     CDS             1659..2054
                     /locus_tag="IVK93_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387823.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            2379..2996
                     /locus_tag="IVK93_002584"
     CDS             2379..2996
                     /locus_tag="IVK93_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6387824.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            3037..3336
                     /locus_tag="IVK93_002585"
     CDS             3037..3336
                     /locus_tag="IVK93_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387825.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            3370..3537
                     /locus_tag="IVK93_002586"
     CDS             3370..3537
                     /locus_tag="IVK93_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6387826.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(3582..4187)
                     /locus_tag="IVK93_002587"
     CDS             complement(3582..4187)
                     /locus_tag="IVK93_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6387827.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            4312..>5759
                     /locus_tag="IVK93_002588"
     CDS             4312..>5759
                     /locus_tag="IVK93_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HAP6387828.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIP"
BASE COUNT         1992 a          849 c         1064 g         1854 t
ORIGIN      
        1 atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa aaggactatt
       61 gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta cgcaattaac
      121 tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta gatatcttac
      181 tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag aggaattgaa
      241 taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag gtgcagcatt
      301 agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag atgcagcaaa
      361 aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag acatgttagg
      421 ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg taatgtcata
      481 tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa taatcaataa
      541 cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg ggaatttagg
      601 ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca taggaagtgg
      661 caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg gatttaatga
      721 gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga aggttataaa
      781 tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc cttggaaccc
      841 tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca tcatttcttc
      901 tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat taactaatag
      961 cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag gtttgcctca
     1021 cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg atagtgtttc
     1081 tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta agaaattagg
     1141 aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg gaacatttag
     1201 tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag caaaaagaaa
     1261 ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg gtgctattga
     1321 tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg cttcagtaac
     1381 aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga taatttcatt
     1441 tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc ttgattttat
     1501 tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat ttcaagaaaa
     1561 tagaagtaaa cttaattaag aatcgtttac tatttaaaca gtgtcctttt cctttttaaa
     1621 tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg gaagaaatag
     1681 aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt atctttatgt
     1741 ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta gattactttg
     1801 cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga gtaccacctt
     1861 atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca aatgaaatgt
     1921 ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa tacaattata
     1981 ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa atatatagtg
     2041 ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa attctgatgt
     2101 gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag tttatttaat
     2161 ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat aatcatgcgg
     2221 gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt ttctttgttt
     2281 atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa tctgattgtt
     2341 ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt ttttatatcc
     2401 gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag gaattaggaa
     2461 tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa cgccctgaat
     2521 atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa tcattagata
     2581 gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact aaggaaaagc
     2641 agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac aagcaactag
     2701 atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat atggccgaag
     2761 acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg aaacaaaagg
     2821 gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg atctattctg
     2881 actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc atgaaacgaa
     2941 atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt tcttgataga
     3001 tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa tcaaagggtc
     3061 gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt ggttcactct
     3121 ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt ttgtggtcag
     3181 tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac cgcgcgggac
     3241 ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta tgcgttaatt
     3301 agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt tacaaggagg
     3361 aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac tagttttaat
     3421 cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag tgtttatcaa
     3481 aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacatgg aacatagaaa
     3541 cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt tctacaaatc attcgctttc
     3601 tttattttat aaatagttgg tcgagagatc ccgttttctt ttgcaatctc tgcaactgga
     3661 atgccttgct ttaacatgtc caccacggtt ttaaaaacta agcgtttttg cggatctgtt
     3721 gagttaatag aatactcgat tggtcgtccc ttatatcggc cggctttttg agccaataat
     3781 actccttgcc gctgacgttc acgaattttc tcacgctcgt tttgtgcaat ataacttagc
     3841 agtgataaaa acatatcgaa catagcggta tccaggagtt catttccagt attaaaatct
     3901 aaaaactgtg catctaatat ttttaacgct acttttttct gtttcataaa agcaactgta
     3961 ttttttatat cctcataatc tcttcctaaa cgatctagtg atgtaacgat gacgcaatca
     4021 ccttcacgga tataatttag taatttttga aactcaggac gctcagtatc tttaccactg
     4081 gctttttctt ggaataattt atcaatgtta agtccttcca tcattgccaa ttgtcgatcc
     4141 aaattttgat cttgtgaaga tactcgaata tagccaattt ttcccataaa aaactccttt
     4201 ttaaaagtga tataatttac atgtaaaatt tacttgtagt tttactgcta tcatagcatt
     4261 tttaaaaata aattttatgt agacttaaaa tttacttcat ggagggttta aatggatgaa
     4321 atcaaaagta acaaaaaagc atttggcatt tatttaggag aacctagtac agaacaatta
     4381 aaccaatttt tttatttgca tgaaaaagat ttagaattga tggagactat acgacgacca
     4441 gctactagat taggttttgc actacagtta gtctcactac gttttttagg aacttttata
     4501 ttagatctta ccaatattcc ccaaaatgta ttgagttatg tagcaactca actaaatatt
     4561 gatcctggag aaattatcta ctatagcagg agacagacca ggtatcagca cacaaaattg
     4621 ataaaatcac attatggata tcaagatttc aaccaaccgg aaatagaaac atacctagct
     4681 aactggcttt taaatagagc gctgtataca acagaatctt cagatatgtt gtttgatatg
     4741 cttctaaaaa aatgtctgga tgaaaagatt gttttacctg gaattactac tttttctcgt
     4801 tttgttgcta gtgcaattga aaaagctgaa gagcatttat accgacaatt gtctttggtt
     4861 ccaactgaag atgagagaaa gcggcttctc aatcatttag aattaattgg aactccagtt
     4921 tatggggcaa taatcaaaat ggacattttg agaaccccgt tagctgacga tagtcataaa
     4981 gaaatagcca gaggttttga ccgactaaag caatttcaag ctttctctac tgaaaaatgg
     5041 aagatggaat ctattcctga agggaaaata aaagttcttg caaattatgc atttaaagct
     5101 aaagctcaat taattcaacg aatgagtgaa cagaaaaaac tggcgttgtt agtggcattt
     5161 attttcatat acaaacgtaa ggctatggat gaacaaattt tagcactatc taattttttt
     5221 gaaactgttt ttagacgcgc caaaaataag gaatctaaag aacgtcttcg taccataaaa
     5281 gatctagatc gagccgcatc gacactttca gatattgtag atttagtaat gtccagtcat
     5341 tctgatacta atgaactatc ccttcgcgat cgtatcttag aaaaatattc tgaggaaaat
     5401 gttaccgaag ctgtatcgca agttcggcta ttggttaaaa atgatcgtga accaatagcc
     5461 atttctgaac ttctgaacac ttatcgaaag ttcagaaaat ttattccgga tattttaact
     5521 acactaacat ttgacagcaa tgactacggt aacaactgta aggaactctg gaccttcatt
     5581 aataatagat ttcctaagcc gattacttac aaaatgtttg gagctattaa acatactctt
     5641 ccaaagaaat ggatttatta cattcatgaa taccctgaac aaactaataa atgtgtattg
     5701 attgcagctg tcgaattgtt aattcaatct ttaaaacgcc atgatattta tattcctca
//
LOCUS       DACCCR010000120         5659 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCCR010000120 DACCCR010000000
VERSION     DACCCR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.130"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..192)
                     /locus_tag="IVK93_002589"
     CDS             complement(<1..192)
                     /locus_tag="IVK93_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387829.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(735..2885)
                     /locus_tag="IVK93_002590"
                     /pseudo
     CDS             complement(735..2885)
                     /locus_tag="IVK93_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3031..3606
                     /locus_tag="IVK93_002591"
     CDS             3031..3606
                     /locus_tag="IVK93_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6387830.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3820..4515
                     /gene="vanR"
                     /locus_tag="IVK93_002592"
     CDS             3820..4515
                     /gene="vanR"
                     /locus_tag="IVK93_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP6387831.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4493..5647
                     /locus_tag="IVK93_002593"
     CDS             4493..5647
                     /locus_tag="IVK93_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6387832.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1769 a         1092 c         1072 g         1726 t
ORIGIN      
        1 ttcaaaatct aaaatcaaca catcatgggg agtgtgataa taaatatttt cccttaatgt
       61 accaacaatg gtaattcggt caattgacca ttgcattttt aagggggtgc acatagtttg
      121 tgcatttaac cgcctgttag aaatacggtt aagtttttcc cactcattta cgattatgcc
      181 ttttttactc aaaaatagct cctcctcaac gaaaaaagag attcttggaa agaatttcca
      241 gagttcacgt aactctaaaa atccacatcc aaaaatctct agcttgaaga aacctaaaaa
      301 tattcgtata atgaacttga gttacggaac taggtgaagt agtttccgta aaggtcgaga
      361 gtgcgtcaac actgctcgac tattttttta ctcttttttc tcattttctt tgattatagc
      421 actcctctag aaaaaataaa aagacctcag ggaatttcat tccccgaacc ccttgagcct
      481 cctcctgccg aaatagaatg gcgctgccag catttatttc gttcagctat cggctacaca
      541 atctaacacg accgttccgc taccagtcgc cttacaggct ccattttcgc tcctctaacg
      601 tgtttcaccg attgtgccga taactaaacg aaataaacgc taaaaccgct cagaacgcaa
      661 aatatgccat tttagagcta ttgtagaaaa tatagtttga aatgcaattt tcttctttca
      721 gctttttatt tcccacttgt aaatcggtag atggtggctc cttttccagt tccataatgt
      781 ggatttgcat ctgcatgtag tgatgaaaca cctagctgca ttctcatacc atctgacgaa
      841 gatgttgtac cgtcgcccca atagaaaggc aattgtaatt tatgatgaaa gtttactaat
      901 atggcttggg ctttattcat ggcatcttca tacatgcgcc attgagatac attggctagt
      961 tgcttatatg taagtccggg tgtggcttcg gccatcttgc tcaagccaat attcattccc
     1021 attcctaaaa gggcagccat gataatgatt gtttcttcct tatctggttt tcgattattg
     1081 gaagcatgag tgaattgctc atgaaatcct gttatatggg ccacatccat gagtaaatca
     1141 gttaatttta ttcttggtag catctgataa aggcttgcac taaatttttt tgcttcttct
     1201 ggaacatctt tttctaagcg tgcaagtgat agctttcctt tttcaagaga aaccccatct
     1261 aacttattgg aattggcagc taaccacttt aacctttcat taaagctgct ggttctctcc
     1321 gttatataat cttcgaatga taaactaact gataatctcg tattcccctt cgattgattc
     1381 catgtatctt ccgaaaacaa atattcctca aaatccctat attgtctgct gccaacaatg
     1441 gaaacatctc ctgcccgaac atgctcccga agttctgtta aaacagccat ttcatagtaa
     1501 tgacgattaa ttgttgtacc atcatcctcg tataaatgtc ttttccatcg ttttgaaata
     1561 aaatccacag gtgagtcatc aggcactttt cgctttccag attcgttcat tcctcggata
     1621 atctcaacag cttgtaaaag tggctcattt gcctttgtag aatgaaattc caatactctt
     1681 aatagcgttg gcgtatattt tcttagtgaa taaaaccgtt tttgcagtaa gtctaaataa
     1741 tcatagtcgg caggacgtgc aagttcctga gcctcttcta ctgaagagac aaaggtattc
     1801 cattcaataa ccgattctaa aaccttaaaa acgtctaatt tttcctctct tgctttaatt
     1861 aatgcttgtc cgatgttcgt aaagtgtata actttctcat ttagcttttt accgttttgt
     1921 ttctggattt cctcttgagc cttacgacct tttgataaca aactaagtat ttgcctatca
     1981 tgaatttcaa acgctttatc cgttagctcc tgagtaagtt gtaataaata gatggttaat
     2041 atcgaataac gtttattttc ttgaaagtca cggaatgcat acggctcgta tcttgagcct
     2101 aagcgagaca gctgcaacag gcggttacgg tgcaaatgac taatttgcac tgtttctaaa
     2161 tccattcctc gtatgtattc gagtcgttct attattttta gaaaagtttc gggtgaagga
     2221 tgacccggtg gctcttttaa ccaacccaat atcgttttat tggattcgga tggatgctgc
     2281 gaggtaataa tcccttcaag cttttctttt tgctcatttg ttagagattt actaaccgta
     2341 ttaaatagct tcttttcagc cattgccctt gcttcccaca ccattctttc aagtgtagtg
     2401 atagcaggca gtataatttt gttttttctt agaaaatcta tgcattcatg cagtagatga
     2461 atggcatcac cattttccaa agctaattga tgaaggtact taaatgtcat tcgatattca
     2521 ctcagggtaa aagttacaaa gtcgtattca cttcgaattt ctttcaaatg atcccaaagt
     2581 gtattttccc tttgaggata atgatcaagc gaggatggac taacaccaat ctgtttcgat
     2641 atatattgta tgaccgaatc tgggatgctt ttgatatgag tgtatggcca accgggatac
     2701 cgaagaacag ctaattgaac agcaaatcct aaacggtttt cttccctcct tcgcttatta
     2761 actatttcta aatcccgttt ggaaaaagtg aagtaggtcc ccagtatcca ttcatcttca
     2821 gggatttgca taaaagcctg tctctgttcc ggtgtaagca attctctacc tctcgcaatt
     2881 ttcattcagt atcattccat ttctgtattt tcaatttatt agttcaatta tatatcaata
     2941 gagtgtactc tattgataca aatgtagtag actgataaaa tcatagttaa gagcgtctca
     3001 taagacttgt ctcaaaaatg aggtgatatt ttgcggaaaa tcggttatat tcgtgtcagt
     3061 tcgactaacc agaatccttc aagacaattt cagcagttga acgagatcgg aatggatatt
     3121 atatatgaag agaaagtttc aggagcaaca aaggatcgcg agcaacttca aaaagtgtta
     3181 gacgatttac aggaagatga catcatttat gttacagact taactcgaat cactcgtagt
     3241 acacaagatc tatttgaatt aatcgataac atacgagata aaaaggcaag tttaaaatca
     3301 ctaaaagata catggcttga tttatcagaa gataatccat acagccaatt cttaattact
     3361 gtaatggctg gtgttaacca attagagcga gatcttattc ggatgagaca acgtgaaggg
     3421 attgaattgg ctaagaaaga aggaaagttt aaaggtcgat taaagaagta tcataaaaat
     3481 cacgcaggaa tgaattatgc ggtaaagcta tataaagaag gaaatatgac tgtaaatcaa
     3541 atttgtgaaa ttactaatgt atctagggct tcattataca ggaaattatc agaagtgaat
     3601 aattagccat tctgtattcc gctaatgggc aatattttta aagaagaaaa ggaaactata
     3661 aaatattaac agcctcctag cgatgccgaa aagccctttg ataaaaaaag aatcatcatc
     3721 ttaagaaatt cttagtcatt tattatgtaa atgcttataa attcggccct ataatctgat
     3781 aaattattaa gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt
     3841 gtggatgatg aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat
     3901 acggttttca aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt
     3961 gaccttgcca tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa
     4021 ataagggaca agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat
     4081 aaaattacag ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg
     4141 gagttaattg ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag
     4201 gagcagaacg aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag
     4261 tgttatctga acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc
     4321 ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc
     4381 gacgaatatt tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa
     4441 aaaatgaacg acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat
     4501 aaaattgaaa aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat
     4561 cgttgcaatt gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg
     4621 gaaacttggg gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga
     4681 cgcgatgaaa ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc
     4741 gattgtcatt agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt
     4801 tgacgagata aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct
     4861 ttctgcggaa atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa
     4921 gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc
     4981 gcacgatatt aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc
     5041 tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta
     5101 tcgactcgaa cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat
     5161 aacgctaaca aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt
     5221 ttatcctcag ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac
     5281 cgtgtccggc gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc
     5341 tgcatacagt gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt
     5401 gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga
     5461 aaagttctat aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt
     5521 ggcgattgca aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga
     5581 taactatacg acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag
     5641 gtcctaagag atgtatata
//
LOCUS       DACCCR010000121         5494 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCCR010000121 DACCCR010000000
VERSION     DACCCR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.131"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(559..1599)
                     /locus_tag="IVK93_002594"
     CDS             complement(559..1599)
                     /locus_tag="IVK93_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6387833.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="IVK93_002595"
     CDS             complement(1948..2274)
                     /locus_tag="IVK93_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387834.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3049)
                     /locus_tag="IVK93_002596"
     CDS             complement(2261..3049)
                     /locus_tag="IVK93_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6387835.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3417..3707)
                     /locus_tag="IVK93_002597"
     CDS             complement(3417..3707)
                     /locus_tag="IVK93_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP6387836.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3843..4022)
                     /locus_tag="IVK93_002598"
     CDS             complement(3843..4022)
                     /locus_tag="IVK93_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387837.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4019..4369)
                     /locus_tag="IVK93_002599"
     CDS             complement(4019..4369)
                     /locus_tag="IVK93_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387838.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4362..>5494)
                     /locus_tag="IVK93_002600"
     CDS             complement(4362..>5494)
                     /locus_tag="IVK93_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP6387839.1"
                     /translation="TNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFRRYVAD
                     EDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVGVGDN
                     PLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMS
                     IRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYT
                     RRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNTK
                     ELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDK
                     IRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
BASE COUNT         1621 a          995 c          807 g         2071 t
ORIGIN      
        1 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
       61 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3421 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3481 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3541 acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct tgcaaccgtt
     3601 ccttgggggt aggcatataa ctcacaaaat tgttctacag tgattcctaa attcttgata
     3661 atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt gcgtaagcct
     3721 actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt ctagtatcta
     3781 ctcctatttg taaaaacaaa aaaggattca tagagataac tctatagaat ccgtaaaata
     3841 ccttatatat ataacgggaa gatataaaac ctaaaattat gataacctgc tgcagcttct
     3901 acatagaaag agaagcgctc aacaccaacc tcatttatca aacgttggcc ataaccccaa
     3961 atctcttctg atagctgatc aaaggttata tactttagct catattgctg gataatgttc
     4021 attctaatcg actccattta ttatcctggt aaaattcgat atgacgtatt tcgtcgtagt
     4081 gaattttgtc ttttccaacg tagatcccca gctcatcact acctaagatc ttgccaatga
     4141 tatcttctgg atagtatcct tcagcgtttc gttcttcttt ttgaatagat actgatctat
     4201 tttttatgac tgcagcgttc aatacttccg caatctcttc agtcgtcatt tgttcttttg
     4261 ctaaattttg actggttcgt tgttcattgt cggcatcaat ttcttcagta tgatcagata
     4321 aataaaaccc tgcccacttt aaacgtccac gatcattgta atcaatcatt tttaattccc
     4381 ccatttccgc ccgcatgacc accaacaagt gtgcttctag taatgcttct agcgccttct
     4441 aacattgaag aagcatgtac aatcgacgta aaaccatatt tttgccgaat cttgtcaata
     4501 attttatcta aattttcttc gtcaatttgc ttttgtggat cactaaataa atccagctga
     4561 agcgaatcgg tgtacacaag cttgctatag gttattccaa catgccttac ttcttgtcct
     4621 tcataatact tatcaaacaa gaaaagacaa taattctgca attcctttgt attatctgtg
     4681 atagggatct tcatctggtg tgaaaaccct ggtctcgttt ctaagatcga cgtgccaatg
     4741 tgtaaatgga cacaacctgt tttacagtta tgctgcctaa tgcgtatcgc tacttgttcc
     4801 gccatttctt ttaccaccag ctcgatttca tttcttctag tataatctcg tggtaacact
     4861 tgtgaatttc catatgattt ttcctttgtc ggttctggtg ggattgctat atctgttcga
     4921 tcgatcccat tagcatggaa gtacagctgt aagccaatca cacctagtct attcttaatc
     4981 gtataaggat tccagttagc aagatctcta atgctcataa tccccatctg attgagtctt
     5041 tttttcattc gagagccaat tccccaaaag tccgtcattt caggaatatt ccatactttt
     5101 tcaggaacgt tttcgtacgt ccattccgca acaaatcctt cattatgctt tgcttcgttg
     5161 tctaaggcta gtttagccaa aaggggatta tctccgacac ctactgtagc aatcaatcct
     5221 agctcttctt taatattctc ctggatcatt tgagccagct tctttctccg ttgacttcgt
     5281 gttccttcag tcgtaaaaag attcagtgat cgggtcactt ttaagattga ttcatcgatc
     5341 gagtaaatca gtagatcttc atcagccaca tatcttctga aaatattatt tacctgcata
     5401 tttcgcttga tatataggtt catacgtggt ggaacaacgt gtagtgtttt aggaaatggt
     5461 tgtggtaagt cacgtggtct actcacattt gtaa
//
LOCUS       DACCCR010000122         5475 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCCR010000122 DACCCR010000000
VERSION     DACCCR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.132"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(37..403)
                     /gene="ssrA"
                     /locus_tag="IVK93_002601"
     tmRNA           complement(37..403)
                     /gene="ssrA"
                     /locus_tag="IVK93_002601"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(574..1347)
                     /locus_tag="IVK93_002602"
     CDS             complement(574..1347)
                     /locus_tag="IVK93_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6387840.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1491..1841
                     /locus_tag="IVK93_002603"
     CDS             1491..1841
                     /locus_tag="IVK93_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP6387841.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1822..2538
                     /locus_tag="IVK93_002604"
     CDS             1822..2538
                     /locus_tag="IVK93_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6387842.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2538..3986
                     /locus_tag="IVK93_002605"
     CDS             2538..3986
                     /locus_tag="IVK93_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6387843.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4056..4565)
                     /locus_tag="IVK93_002606"
     CDS             complement(4056..4565)
                     /locus_tag="IVK93_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387844.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4555..5280)
                     /locus_tag="IVK93_002607"
     CDS             complement(4555..5280)
                     /locus_tag="IVK93_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387845.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1859 a          955 c         1095 g         1566 t
ORIGIN      
        1 ggatttaagc atctagcgaa atctgctgaa atctcacaat ggagacggcg ggagtcgaac
       61 ccgcgtccaa acacatcgcc acttaaaaat ctacgttcat agtcaactca tttaaggatt
      121 cactttacag cttgccgagt gacaggcatt ctgtatcgct agtctgatgt tctctttttc
      181 aacttacaga cggaaagttg aaacgtatcc cactaaagtt aggaccctta ctcgagcaca
      241 tgggcgatgc cgagaggatc tacgctaact gtttttaggc agctaaagcg taagagttgt
      301 tttcgttttt agcagttata tttaactgta acgtttttac gtagacgtaa cctacgaaac
      361 gcaattcaag ctcgactgtg cctgtcgaat ccgtaacgtc cccgttagct aacaagggtt
      421 acgtgtattt ttcatacaaa attagtatag catatattgg gataaattca ataaaaaagc
      481 gaatcatatc ccattattta tataataact gcccctttct taagaaaaaa taagcgattt
      541 tcttaagaaa agaggtagct tcaaaaaaag atcttaaatc agatcaaaat acttcaatgc
      601 gtggacaatt ccgccttctg tatttttctt tgtgataaag gtagaaagtt cttttaatcc
      661 gtcataggcg ttgcccatag cgattttgtt gtcgcaagct tccaacaagg caaaatcatt
      721 cggaccatcg ccaaatccaa aagtaggaac tttttccagc tgaagttctt tttgcagacg
      781 tttgacacct gccccttttg agattccttt cttcactgta tcgatcgagt aaggaccatt
      841 ccgataaaaa gttagctcag gaaaatgttc gtgatagtat tcgtcacctg attgagataa
      901 gatcagcaac atatttactg tgttttcttt aaagcctaaa ggatcaattt ccggaacatc
      961 tgaatgaatg aagtcgtaag cattgatcat tgtttggcta tgaccggtac accagatttt
     1021 ctgatcgtta tagtaagaaa gttcatgacc gttcaactgt acatgatcat acattttttg
     1081 acattcttct gaagaaaatg tttgagaata gatttcttcg ccgtctactc gaatgaaaga
     1141 accattcatg gtgaccgcag aagtaatgcc cgcatcttcc atgatagagt ggatttctgc
     1201 ttctgtacgt cctgtagcga tgacggggag aacattgttt tcttttaatg cgttcatagc
     1261 ttttgccact tcgggtgtga tttttgattt cgcatctagt aatgttccat caagatcaaa
     1321 gaaagtgatt gcacgataat ttgacacaaa taaaactcct tttttattgg aataagttta
     1381 tcttaacaaa gaatgaacaa aagacaaacc tttcgtgaag aattcctgaa catattggtt
     1441 cagtcaacaa tttagagtat actataagaa gagaatttag gagggaaaga atgatttttt
     1501 tcagtattgg tatcttattc ttaataattg gcagtctttt tcggattctg ccctcaaaag
     1561 gaaatctgcc tttttatggg tatcattcgc ctcttgccgc aaaaacagac gctcattggc
     1621 ggctggctca aaagacaagc ggaaactggt tttttcttat gggcttactg atggcattga
     1681 tcggctatta tttaaagaca tctgggcata cgaattattt tctgattgaa atgcttctgc
     1741 tagtatttcc aattatgcct atttttatta tgacagaaaa gaaattacag aaatatgacc
     1801 tggaaactgg agggaacgat aatgaatatt ttaatgattg aagataatca atctgtttct
     1861 gaaatgatgc agatgttttt cttaaatgaa ggttgggaag caactttcaa gtatgatgga
     1921 aaagaagggc tcgatgcctt tttagaaaac ccacaacatt gggatatgat tacactagac
     1981 ctgaatttgc ccacactgga cggtatgcta gtaggaagag agatccgcaa agtttcaagt
     2041 actgtgccga ttatcatgct gacagctaga gattctgaaa gcgatcaggt gatcggctta
     2101 gagatggggg cagatgatta tgtcacaaaa cctttcagtc ctctgacgct tattgctcga
     2161 atgaaagctt tgcatcgccg tagtgagttg gcagaagcaa gagaagaagc agtagtttca
     2221 gatgatcatt ttgatatcga gactagtcat ttcaagatga atacgaaaac acgagaagcg
     2281 tatctggaca accggccaat tgaaggactg acgcctaaag agttcgatct attgtttact
     2341 ttagcaaaaa aaccacgtca agtattctct cgggagcagt tgcttgaatt agtctgggat
     2401 tatcagtact ttggtgatga acggacagtg gacgcccata tcaagaaatt acgacaaaaa
     2461 atcgaaaagg tcggaccaca agtcatccaa actgtttggg gagtcggcta caaattcgat
     2521 gattctggtg ttgcctaatg cgttatttgt atcagcaact cttagctttc tgcggaatgg
     2581 tcgcgttgat cttattgatc gttggagttt cttttacaca actgacaaaa caaacgattg
     2641 aagaaaacaa ttaccagcaa ctgtttggat atgctgaatc agttgaaaag acaacgcaaa
     2701 cctatgccga tacgctcccg cagtttagtc aagatcaggc gtttcaaaat gcattgctgc
     2761 tgacagagca agtactgaca gaacaaaatg tgaactttat atttatcgat aaaaacgaac
     2821 gtgtcattta tccgactaat acagctttac tgaattttac cttaacagct gatcaatgga
     2881 aaaatctgag gaatggacgt caagaaaaat tcacatcgaa taaaaatatt ttaggacaag
     2941 cacaggcgac ctcttacgca cttgtgccat ttaatttgaa tcatgaattc tatggagcac
     3001 ttgtagttag tcaacctgcg cgaaatatcg ataacagcgt tcgtccaatt acactgaacc
     3061 tgttcaaagg atttattttt tccagtatcg ttgcagttat tgccagctat gcttttgcag
     3121 cattccaagt taagcgtatc aatcggttaa ggaatgcgac gaaagaagtg acgaatggta
     3181 attttgatgt acagctcccc gtacatgaca aagatgaatt tgatgagtta gcagatgatt
     3241 tcaataaaat gacgaattca ttgaaagaat ctcaagcaga aattgaagaa caagaaaatc
     3301 gtcgaagaca atttatggca gatgcttctc acgagatgag aacacctctt acgacaatca
     3361 acggacttct ggaagggtta gaatacaatg caatccctga aaatcagcga gaaaacgcga
     3421 ttaaattgat gaaaaatgaa acagagcgtc tgatccgttt ggtgaatgaa aacttggatt
     3481 atgaaaaaat ccgtacgaat caaatcagta tggtcattaa aaaattcaat ggaacagaaa
     3541 cattgaaaaa tattgttgca cagttagaag ctaaagcaga agcagcagga gatacattga
     3601 cattgaaagc cgttgatgat attgatgttt acgctgatta tgaccgattt gtccaaatca
     3661 tggtcaatat tattcaaaac gcgatacagt ttactgaaaa cggccaaatc acgattactt
     3721 tagaaaaagg ctatcttgaa acgatcatca cgattgaaga tacgggaatt ggaatgtctg
     3781 aacaacaaat gaaaagtatc tgggatcgtt attataaagt agatccttca agaaaaaata
     3841 caaaatatgg tgaatcagga ttaggtttac caatcgtgca gcaacttgta cgcttgcata
     3901 aaggaaaatt agaagtagaa agcgaactag gaaaaggaac aaaattcgct gttcgtttgc
     3961 ctgacgtaga aatcaaaaaa gagtaaaaaa caagcggtat aaaagatcat gaatcgatct
     4021 tctataccgc ttgtttttac tataaaatga ttgatttagc ccgcgggctc taaacgatca
     4081 atataaggac tttgagcaat agtagcatca tgagtaaaga gtaagatacc ttgattttct
     4141 tcatgagcta aataagaaag caagtggagt agctcttgga tctccaatac actaagttca
     4201 tcaaatgtat cttcaataac aatattgttt cgcttggaaa gcaaggcatg aagtaattgc
     4261 agctttactt tttcaaaaga cgtcaactca gctgccgcac aattaagcaa ggaaggagag
     4321 atatcaagtt tttcttgata ggccaaacga tcttgtttga tttctttttc cggtacatcc
     4381 aacaggacat ggtctttaag attcagataa ggaaacatcg gccaattttt ttggatgatc
     4441 gcaaaattat ccagttttgc aacaagaacc tcttttttca agtgctccaa tattttatgc
     4501 tgtttttcga gaggacacaa aataacggtc aagtgttcag catgaatcaa atattcaggt
     4561 ttcattacgt ccaggcctcc tactttgcaa atgatggata aaaaaagtga aagggaatag
     4621 actaagaatg agacagcatc cactgagccc aataagagtt tgggcaaatc ccttaagtat
     4681 atctgtgaaa aaaagatcat tttggaaatc gatggtaaaa aaagtctgtt gatccatcgg
     4741 aattgtaagt ccgcttaccg aagtcaagat atcacggata tctggcagtt cagtagtctt
     4801 taagacaaga aaattgatcc tttgaagaag cgcttcataa actgactgga agcttacgat
     4861 ccacaataaa accaagatac aacaaagaaa gagaggaaag aacagctgcc agaaaataaa
     4921 aaggaataat ttggttttag aaagaccagt caggtgccaa ttgatcaatt cctgcttgtt
     4981 ttgcctgact gaatagtaaa agaaaaagat aaaaaggaag gcaaatagaa aaaataaact
     5041 gaatatgcct tttatataaa atcggctagc taattctaaa gccggaagaa gctctgtaga
     5101 tgcagaagcg ccaataaatg aaagccgatc ttggacttga ttggctaaag ccgattgcaa
     5161 atggtaaaga gtcaggcatg ctatcaataa aaaagcagaa aagcttgaaa ataaacaata
     5221 gagtattgtc tgctttttgt aataaattaa tcgagcagta gcatagcgga ttattttcat
     5281 acttttcacc tctataaaaa gtatagaaaa tgaatatgaa taaagtatga aattccttta
     5341 gtgaaatcac gaattaaaac cagaaaatat tttgtaaaaa gcaagaggtt gtgaagaagc
     5401 tgccctattc tattaccgta gatgcaagct tttgaacact gtttattcga aaagaggagc
     5461 agcgacaata atttt
//
LOCUS       DACCCR010000123         5466 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCCR010000123 DACCCR010000000
VERSION     DACCCR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.133"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            133..1107
                     /locus_tag="IVK93_002608"
     CDS             133..1107
                     /locus_tag="IVK93_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6387846.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="IVK93_002609"
     CDS             1089..1550
                     /locus_tag="IVK93_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6387847.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="IVK93_002610"
     CDS             1600..2064
                     /locus_tag="IVK93_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6387848.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="IVK93_002611"
     CDS             2078..2827
                     /locus_tag="IVK93_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6387849.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="IVK93_002612"
     CDS             2827..3657
                     /locus_tag="IVK93_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6387850.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="IVK93_002613"
     CDS             3657..4304
                     /locus_tag="IVK93_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6387851.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="IVK93_002614"
     CDS             complement(4402..5178)
                     /locus_tag="IVK93_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6387852.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5260..5403
                     /locus_tag="IVK93_002615"
                     /pseudo
     CDS             5260..5403
                     /locus_tag="IVK93_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1871 a          739 c          974 g         1882 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg gaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DACCCR010000124         5265 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCCR010000124 DACCCR010000000
VERSION     DACCCR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.134"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(26..1174)
                     /gene="nagA"
                     /locus_tag="IVK93_002616"
     CDS             complement(26..1174)
                     /gene="nagA"
                     /locus_tag="IVK93_002616"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP6387853.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1250..2086)
                     /locus_tag="IVK93_002617"
     CDS             complement(1250..2086)
                     /locus_tag="IVK93_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387854.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2270..3487)
                     /locus_tag="IVK93_002618"
     CDS             complement(2270..3487)
                     /locus_tag="IVK93_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6387855.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3870..3953
                     /locus_tag="IVK93_002619"
     tRNA            3870..3953
                     /locus_tag="IVK93_002619"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3904..3906,aa:Leu,seq:gag)
     gene            complement(4193..5191)
                     /locus_tag="IVK93_002620"
     CDS             complement(4193..5191)
                     /locus_tag="IVK93_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387856.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1630 a         1074 c          835 g         1726 t
ORIGIN      
        1 agttttaagg ttaacttaaa ttatattatg cgtggaaacg ttccacgcca tctagataag
       61 ttgcttccaa tgtcatatca gggttcaata cgataaagtc tgcatcgcgt ccttgagcga
      121 tttttccaca tgtatcatcg attttacagc tgattgctgg aatcagacta gccatcatga
      181 ctgcttgttc tggtgttgct aatccccagt ctacaacatt tttaattgct tctttcaatt
      241 tcaatatact tccagctaag ttgccagatt ctagacgtgc tgttccttct tttactacta
      301 ctggaaattc gcctaaattg taatttccat caggcatccc accggccatc atacagtctg
      361 taattaatgc gacatgatca tgtcctgctt tttccattag gattcccgca gcatttggat
      421 gtacatgatg gccatcacaa atcaattcag aaaagacatg ttctaaagat aataacgcac
      481 ccaccatacc tggttcgcga tgattcaatc cacgcatacc attgaaagca tggacaaata
      541 cacttgcgcc tgcttcaaca gctttttgcg cttcttctag tgtagcatta ctgtgtccta
      601 gagaaacaac tactccttct tcagtcactt gtttgacaaa ctcttctaca ccttgacgtt
      661 ccggtgctaa agcaattttt ttgattaaac cacctgaagc ttcttgccat tcatggaaag
      721 tatccaaatc aggatccccg aaataacttg ggttttgtgc ccctttgtgt tcttctgtga
      781 agaaaggacc ttcaaaatag attcctttga ttttagctcc tggtgcttcc tgatacattt
      841 tgccgattgt ctcagctaca tcttttaaac gttctttgct tgacgtcaat gttgttggta
      901 aaaatgaagt cactccacaa gataacagac cttcagacat cactttgatg ccttctgcat
      961 cattatccat tacatcatga ttcatatagc cgtgaatatg tgtatctacc aaaccaggag
     1021 caatccattt gccgctttgg tcaatgatgt tgacttctcc ctcaggtagt tctttggtgt
     1081 attcaccaaa aagtccatcg gtaatttcaa gatagcctgc atgtttgaca cctgacgcta
     1141 aaaagaattt gtcagcatag ataaaagttc tcattctatt ctctcccttt gtttttctac
     1201 ctctaaagta ctcttattta gagaagaagt caagaatggt ctacaccatt tacaaatttt
     1261 ttgaaatttg tttcacagct tgtgctaagc cttcatagaa tgctgctctt tctggttctt
     1321 ctttacgctt gtatattaag cttttaagca caaataaatc attgaggtaa tagtagctat
     1381 tcttctcttg agcccaatag attccttgat tcacctgttc taacgcttcg tcgatttcat
     1441 tttgatggaa taagaaattt ccataattat aaaaaatttg cgaaaattca ctttttgatc
     1501 gttcatcagc tgattgatgc agcagttgga tgcttcgact caaatagtag cgtgcttcag
     1561 ccgtttttcc tgccacacca tatatttttc cgatacaact aatcagttgt atttctgtgt
     1621 cagaaacatg gattctttct ttttggctag agtagcttaa tgcttctttc aaataataaa
     1681 gggactgaga gatattttga cgcaacgtga attcgcaaac accgagataa taataatagc
     1741 gttgaaagtc agttgcttga tacagatttt cagaaatatc agaagatttt agtacttgtt
     1801 ctaacttccg ataatcacgt ttttggaaac aactatccaa cagttcaaat aatttatcgt
     1861 ttgtccgagt agtttttcga gagagattca tcacatgatc aatcgttacc cctagccgtt
     1921 gacaaatctg catcatcacc aatacattcg gcagttcctc attattttcg atccgactta
     1981 atacactttg tgaacaaata tcatgcgaaa gcattttttg cgtcattttt tgttcttttc
     2041 gaatctcttt aatgatcgtt ccaaaggact cgattaacgt ttccataagt atcctcccca
     2101 aaatatgcgt attcgcattc tattcgtatt ttactttagg aagtcgcaaa cttcaagaaa
     2161 agacttgcaa ctaaataata tactttagtt atttaagaaa actccactag aaagagagcc
     2221 tcagtgtgtt tgtctgtggc cctcctactg aaaaatttgg tttttaaaat catttttggc
     2281 gataggttgc taggaattct ctcattttct tagctgtttg tttcaaatca tccattttag
     2341 gaagataaac gattcgaaaa tgatctggct ggttccaatt aaatccacca ccatgtacaa
     2401 gtagaatatg atgttcatgt aaaaagtcca aaacgaattt ttcatcatcc aaaatattga
     2461 atcgctttgt atcaattttt gggaatatat aaaaagcagc ttttggtttt acggctgaaa
     2521 gaccaggaat atcattgatt gcattataga tatattctct ttgttcatag atccttccgc
     2581 ctggcaggag cagctcatcg acactttgat aaccgcctag cgcggtttgg ataatttgct
     2641 gagacaatac attggaacac aaacgcatgg aggacaacat attcagccct tctatatagc
     2701 ctttgacgtg tgacttatct ccacttagga ccatccagcc tactcgaaat cctgctacac
     2761 gatgagattt ggatagaccg ttcagcgtaa caacgaacaa atcaggcgct aatgtagcaa
     2821 tcggaatatg ttctagacca tccatcacta agcgatcata gatttcgtcg gaataaataa
     2881 tcaggttatt ttgtcgagct acttcaacga tttcttctaa taattctttt ggataaagtg
     2941 ccccagtagg attattagga ttaatgataa caatagcttt tgtccgactc gttatttttg
     3001 atttgatatc atcaatatca ggataccatt ctgcctcttc atcgcaaata taatgaacag
     3061 gtgtgcctcc agctaatgca atagaagccg tccacaacgg atagtccggc attggaacca
     3121 gtacttcatc tccattgtcc agcaacccct gcatacacat cgtgatcaat tcgctcactc
     3181 cgttgccggt gtagatatca ttgatcgtca cattcggaaa atgtttcagt tggcaatatt
     3241 gctcaattgc ttttctagca gaaaagatcc ccttggaatc tgagtagcct tctgagtcac
     3301 gggcattcat gatcaaatca cgaatgactt cgtttggtgc ttcaaatcca aaaggtgcgg
     3361 gatttcccgt gttcaatttt aaaatactaa tgccctcttc ttgcatccgc tccgcttctt
     3421 ctagaacggg accgcgaaca tcgtaactga caccttctaa tttgttggat ttttcaaatt
     3481 ctctcatcac ttagccaatc cctcatttca aaaagttaga actttccaaa ttttttgtta
     3541 tttactattt caatatcata ctccatccta gaaaattttg ccacttattt aggaaaaacg
     3601 taagtattaa aaaaacacaa acaagcgttg agaacttaat tgtcatgata gtcattccat
     3661 agattcgctg cccaatccat tgcaattcca caaatgattc ctagcataaa accaagcaaa
     3721 aaacgtccga atgcaagtcc aatcaataca cctaagatca ttcccagtcc tgaaaattct
     3781 ccgtcatatt tctccatctt aataagtcct cccatttttg agcttgacaa ctatgtaaaa
     3841 cctcgctatc attagtgtgt tattattacg cagatgtgtt ggaattggca gacaagcatg
     3901 attgagggtc atgtgagcgt tagctcgtgt gggttcgagt cccatcatct gcactacaac
     3961 tcgtgaagtt aattttgctt cacgagtttt tttgtttgaa cgtttcttta tgccattata
     4021 tacatatcac tgtatccttt acaaaaagaa cgtacaatca tgtaaacagc ctgaggtgag
     4081 tatatgaagg tcggaaacaa gctactcgta gaaacttgca agtgttaaaa caattaatcg
     4141 aaaaacgagt aaaatccctc acctaatact aaagcgaggg attttttgta tattatctgt
     4201 ttatcaccgt tattataaag gtacaaatac caagcacggt taatccaaat tgcataaatt
     4261 catttttttg tacactcatg cttttcatta gctctttaac tgccattagt actaagatgg
     4321 cgataacaac ataaagtata tagtacgtcc gcattccgga agcccatact gtgggagaaa
     4381 atcccataat taaccttgaa caaaatccca ttatcatcaa aataactaaa aaaatagaag
     4441 ttttcttatt gtcaaaactt aaataaattc caacaattaa acatagtaat aatgccaaga
     4501 ctactaaagt agctatccaa gtacctggat aaaaaagtga cagtttcgtt cctaactctg
     4561 taaagagatt attcaggtta ctactactcc aaatcattgc ccttttattt ccattgacgt
     4621 aagtaaagct ttgacccatt gtattaccaa aataaataat caggtttaag aaaaacggta
     4681 atgcagtcaa tatacgcaca taatagtttt gacatttcct atatgttaga aggaaaatta
     4741 agaaaaatag caataaaaat aagatattca tgtctaaaaa aaacggcttt ccaaatgatg
     4801 aaatacctaa atctaactta ttaagaaagt tgaaattttt atattcagga aaccatttat
     4861 taatttcatg tgcagcacgt aatgcattcc ctggagttgt caaagagaaa attaattctg
     4921 ctaagcttat tattgaaaat actgataact tataattata tcgtccattg acaatcaaat
     4981 ataaactaac tatacttgtt agaacaaaaa aacaggcgtt gacttgttct tgattagtta
     5041 aaaatatcaa taaaggaata cttacccaat atatttttct atttatttct tctcctctta
     5101 agagttgaaa aaacggataa aaagttagta aactaaatgc tactggccac tgatagttca
     5161 aggtggttgc tctccaaccg gtttccccca ttactgtcaa tggtattaaa cagtatatag
     5221 agcaaaccaa taataaattt ttaacattta ttttctcaaa aacat
//
LOCUS       DACCCR010000125         4930 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCCR010000125 DACCCR010000000
VERSION     DACCCR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.138"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            83..970
                     /locus_tag="IVK93_002621"
     CDS             83..970
                     /locus_tag="IVK93_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6387857.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IVK93_002622"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IVK93_002622"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP6387858.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IVK93_002623"
     CDS             2272..2718
                     /locus_tag="IVK93_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6387859.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IVK93_002624"
     CDS             2991..4343
                     /locus_tag="IVK93_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP6387860.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IVK93_002625"
     CDS             4514..4801
                     /locus_tag="IVK93_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387861.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1394 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt
//
LOCUS       DACCCR010000126         4809 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCCR010000126 DACCCR010000000
VERSION     DACCCR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.139"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="IVK93_002626"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="IVK93_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP6387862.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3252..3770)
                     /locus_tag="IVK93_002627"
     CDS             complement(3252..3770)
                     /locus_tag="IVK93_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP6387863.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1580 a          786 c          730 g         1713 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccactta
//
LOCUS       DACCCR010000127         4782 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCCR010000127 DACCCR010000000
VERSION     DACCCR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.140"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(207..1169)
                     /locus_tag="IVK93_002628"
     CDS             complement(207..1169)
                     /locus_tag="IVK93_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6387864.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IVK93_002629"
     CDS             complement(1171..2610)
                     /locus_tag="IVK93_002629"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP6387865.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLFFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IVK93_002630"
     CDS             2795..4741
                     /locus_tag="IVK93_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP6387866.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1522 a          820 c          788 g         1652 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aaaaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa at
//
LOCUS       DACCCR010000128         4663 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCCR010000128 DACCCR010000000
VERSION     DACCCR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.141"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..303)
                     /locus_tag="IVK93_002631"
     CDS             complement(<1..303)
                     /locus_tag="IVK93_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387867.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYH"
     gene            complement(306..983)
                     /locus_tag="IVK93_002632"
     CDS             complement(306..983)
                     /locus_tag="IVK93_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387868.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            1247..3013
                     /locus_tag="IVK93_002633"
     CDS             1247..3013
                     /locus_tag="IVK93_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP6387869.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3028..3465
                     /locus_tag="IVK93_002634"
     CDS             3028..3465
                     /locus_tag="IVK93_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6387870.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            3478..4629
                     /locus_tag="IVK93_002635"
     CDS             3478..4629
                     /locus_tag="IVK93_002635"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP6387871.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1553 a          826 c          962 g         1322 t
ORIGIN      
        1 atgataagtt gcttcgacca tttgctggac attccctgtg ttttttgcat gtaagattgc
       61 ttcgatcagt ttgttgcttt tctctaaact ttccacaaat tctttctcat tctcatcttc
      121 tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta tgattcagtt catcgaattc
      181 tgtataagca agtttatgat caaaaaaaag tgggaatgca aagagcacga ccaacaatag
      241 aggaaataaa aagtttactt tgttttttag taagagattg cgttcaaatg taagtaaaga
      301 catagttaca cctcatataa tttacgataa atagctaaaa tctcttccat atttgaggat
      361 tctgtgacga tttctccatt ttttagaaaa catgctttag aacaaatcga ttggatattc
      421 gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt tctgccattg tctgatgagg
      481 ctattcaaga tatcaatact tcctgggtct aatccgttca acggttcatc gaataacatg
      541 acagatgaat cactgatcaa gtaagcagca agcaagacat gttgtttcat tcctagagac
      601 atttttccaa tcggcaaagt aaggtactca gtcatcttca atcgttggaa aacagtttgg
      661 cattctactt tgctctgcca atagtatttg atcaaagtca aataatcaac agccgataga
      721 tagtcggata actgattcgt tgattctaga aaaaagaaag accggcgata atctgtataa
      781 gcagtaggag agatgttgtg aagggacaat tgatcataag ttgttggcag cagttgggca
      841 atgccattca gcaaagttgt tttgccaaat ccgttcgggg caatcaaagc agctacttct
      901 cctgattcta ctaaaagatt caattgtcga atcacacgtt ttttaccacg tttcatgttc
      961 atgtttttga tcagtaatgt catagatagg cctccaatat aaaatttaat aacataatag
     1021 aatggaaaac atctaaatta aatgctaaca aaagttattg tttgcatatt ataaaaaaat
     1081 taaaataaca gttttattta aaattgaagt tttctgatac cgaaatcttt ttattttgct
     1141 gacatactca aacctttggc acaaagtttc gtgccaaagt acgactattt ctctttttag
     1201 taagcggata caattagagt atcaaaagga gagagggaga gacaaaatgg aacggacgat
     1261 tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa ttaggcagta ctttatcaag
     1321 catggtcatg ccaaatattg gagcactgat tgcctggggt gtattaacgg ctctatttat
     1381 cccagatggt tatttaccaa atgaggcttt ggctaccatg gtaagcccaa tgctaactta
     1441 tctgattcct ttattgatcg gttatactgg cggaaaagta attgctggtg accgcggatc
     1501 agttgttggg gcaattgcaa cgatgggtgt gattgtcgga acagatattc caatgatgct
     1561 aggcgcgatg atcatgggac ctcttggtgg atatgccatc aagaaatttg atcagctatt
     1621 ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat aatttttcag caggtcttat
     1681 cggttttgga ttagcgttat taggtttttc tgcgattggt ccagttgtcg atgcattgac
     1741 tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc catttgattc cattgacaag
     1801 tatttttatt gaaccagcaa aaatcttatt tttgaataat gcaatcaatc atgggatttt
     1861 gacaccattg ggaactgaac aagtagcaga ggctggaaaa tctattctat tcttgcttga
     1921 agcaaatcct ggtccaggac ttggcgtatt acttgctttt actttgtttg gcaaaggagc
     1981 agcaaaatct tcagcaccag gagccatgat cattcacttt ttaggcggga tccatgaaat
     2041 ctattttcct tacgttatga tgaaaccgtt attattctta tcagttatag gcggaggaat
     2101 gtcaggaagt ttcattttcc aattgatgga tgctggacta agagcgccag cttctccagg
     2161 gtcgatcatt gcgattctag cgatgacacc gttcaattct taccttcctg ttattttggg
     2221 cgtgattgtt gccgcagctg tttcgtttgg tatttctgca gtaattttga aagcggatgc
     2281 gaaagataca aatgaagact tcgaaaaatc tgttcaagag acaaaggcag ctaagcaagc
     2341 ttccaaaggt gcccaaggta ttcctcaagg agctacattc tctggcgtac aaaaaatcat
     2401 ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc gcttcgatct tgagaaaaaa
     2461 agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa gcaatcagcc aactgacaga
     2521 tgactcgaat acattgatcg ttactcaggt cgaactacaa gaaagagcaa aacaaaaagc
     2581 accgagcgcg cgttttgtct cagtagaaaa ctttttgaat tcacctcgat atgatgaaat
     2641 cattcaagaa ttaagcagca agcagaaaac atcagtttct gaaattgaga atgagccagc
     2701 agaagagttt ctttcagcaa acatcaaaca aatcaaagag attctacttg tccacgatga
     2761 tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa gatatattag acaagaacca
     2821 tttaactatc ccgttaagaa aagtcaatat ccaagattta aaagatgaac ctgattcact
     2881 gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg gctgcacctc aatccattca
     2941 tttatcagtc aacagcatgg tcaatccgca aaaatacgaa tcgattgcac aatatttgaa
     3001 acagacagct taagggagga aaacagcatg caaagtttgc cattcgacat gatcgagctc
     3061 aatcgatctt ttaaaaccaa agaagaagcg atccgctatt gcggtcagat gcttgtagaa
     3121 gcagggtgcg ttgatgagga atatatcgat gccatggtag atagagacaa aatgttatct
     3181 gtttatatgg ggaattttat cgcgattcct catggaacag atgaagcaaa aaaatatgtg
     3241 aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg tagattttgg taccgaagaa
     3301 gaagaaaaga ttgcgactgt tttgttcggt attgcaggag tcggagatga gcatttgcaa
     3361 cttgtacagc aaatcgcact ttattgcagt gatatggaca atgttgtcca gctagcagac
     3421 gcgctcagca aagaagaagt aacggaaaat ctagcaattg cttaaggaga gagaaaaatg
     3481 agagcagtac attttggtgc aggaaatatc ggtcgaggat ttattggaga ggttttggca
     3541 gctaatggat ttgagattac tttcgtagat gtgaatcaca cgatcattga tgctttaaat
     3601 gaaagaaaag aatacgaaat cgaattagca gatgaatcaa aagaaaaaat cactgtaaaa
     3661 aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag taatccaaga aatcgctgaa
     3721 gcagatttag ttactaccgc cattggcccg aatatcttac cttttattgc tgagttgatt
     3781 gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa catctttaga tatcattgcc
     3841 tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag aagtgtataa atatcttccg
     3901 gaaacatcat ttactgacaa atatatcggc tttccaaatg cagcagtcga tcgaatcgtc
     3961 ccattacaac accatgaaga tcctttattc gtccaagtgg aacctttcaa agaatgggtc
     4021 attgacgata gccaaagaaa aaataaaaaa atccaattaa aaggggtttt atatgtagat
     4081 gatctagaac catatatcga acgcaaacta tttagtgtca atactggtca tgcgactgtc
     4141 gcttatacag gtgccttgtt aggttaccag accattgatg aagcaatgca agatgcgtta
     4201 gttgtggcac aattaaaatc agttttgcag gaaacaggaa gtttgctgat tgccaaatgg
     4261 gggtttgatg cagagcagca tcatgcgtat attgaaaaaa tcattcatcg cttccaaaac
     4321 aaaaatatct ctgatgcgat cacacgtgtc gctcggacac cattacgtaa gttaggttat
     4381 caagaaagat ttacacgtcc agtgagagaa ctacaagaac ataacttgac ttgcccacat
     4441 ttgacggcaa caatggggat tatttttaat tactatgatc ctgaagatga acaaagccgt
     4501 caattgcatg aaatgaagat ccatgaaaac ttggaacaat tgattcaaga agtcacagga
     4561 atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg tgaaccgtta tgccaagcaa
     4621 gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa att
//
LOCUS       DACCCR010000129         4661 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCCR010000129 DACCCR010000000
VERSION     DACCCR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.142"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..197)
                     /locus_tag="IVK93_002636"
     CDS             complement(<1..197)
                     /locus_tag="IVK93_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP6387872.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGIT"
     gene            647..1261
                     /locus_tag="IVK93_002637"
     CDS             647..1261
                     /locus_tag="IVK93_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6387873.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1426..2430)
                     /locus_tag="IVK93_002638"
     CDS             complement(1426..2430)
                     /locus_tag="IVK93_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387874.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(2889..3146)
                     /locus_tag="IVK93_002639"
     CDS             complement(2889..3146)
                     /locus_tag="IVK93_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6387875.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(3139..3408)
                     /locus_tag="IVK93_002640"
     CDS             complement(3139..3408)
                     /locus_tag="IVK93_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6387876.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(3539..3718)
                     /locus_tag="IVK93_002641"
     CDS             complement(3539..3718)
                     /locus_tag="IVK93_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387877.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(4099..>4661)
                     /locus_tag="IVK93_002642"
     CDS             complement(4099..>4661)
                     /locus_tag="IVK93_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387878.1"
                     /translation="QLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEKY
                     IMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDDY
                     YLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTY
                     DDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         1482 a          857 c          653 g         1669 t
ORIGIN      
        1 gtaataccat accgcttttt tgcttcagga gaagaagcta atatcaatcc tgaaccagta
       61 ttatcagctc gactcataac aacaagttct gttgttaatg gatctaaatt tctttctata
      121 cattcgacac ttgcgtaaaa gcttttcata tcgattaaaa agtaatcatt tactggctct
      181 tttgaataat ccatcataaa taacactcca ttctttcaca ttatacaaac atatgttcga
      241 tattgtaaag aagaaatagc taaatacata aaaaaagaag cataggtatg ctatcaccct
      301 gcttcttctt ttgatttgta tgatcaattt attctatcac tttactaatt aatttgaaaa
      361 tctaataaaa actctcactc taaaagagtg agagactgca aattagagta cggacttttt
      421 tggagttccg ttctttaata ttaacaaatg aataaaatcc ttacaacatt tgagaataag
      481 ttatcagtag acacttacaa aaaattctca tactatttcc tccatcatta gtttttgtct
      541 atataaggtt taccctaaat agacactggt ataacattga aaaatatggc ttcctcgtgt
      601 atgattaggg tgtattttaa atagttgcag aaagtaggta aaagttttga gagttgcata
      661 catacgtgtc tcatcgatcg atcaaaatga acaacgccag attgaagaaa tgaaaaaatt
      721 cggtgcagag agaattttca tcgagaaaca atctggcgca acgattactc atcgacctgt
      781 tttccaagaa gcattagatt acgtccgaga tcaagatatt tttattgtgg aagctattga
      841 tcgacttgga agaaattatg acgagatcat cgcttcagtc aattacttga agaagaaaaa
      901 tgtgaagctg atcattacta gtttaccgat catggctgaa gcaattggca atcctctcct
      961 ggataaattc attaaggatc tgatcatcca aattttggcc atgatcgctg aacaagaacg
     1021 gactgaatcg aaacgtcgcc aggcacaagg aattaagatc gcaaaagcaa acggcgtcta
     1081 caaagggcgg cccaaattat acagtgcaga cgcaaaagat ccccaacgtc gccttgttta
     1141 caaaagcatc gttgaagatc ttaaaaatgg tgttgcgatt gctaagattg ctaaagacta
     1201 caatgttacg agacaaaccg tgtatagaat aaaaaaagac agcatggtca atgacaaata
     1261 gttgatcatg ctgcttttat tatcttctca tctttaattt cctacatata attactatct
     1321 ataaaattaa gtcttactca gaacagataa agcataaaat tccaacaaac cattttttac
     1381 ttaactaaat aaagagtctg ttaatataat cttcacttat ttacgttaat cccctactgt
     1441 atttgttggt tccttatcta tattttctac aaaatcctta ttatcttgtg atttagcttc
     1501 gtataattct ttgtatgtcg acacattggt tatactaatt cctatagcta gttcttgaga
     1561 ctctactttt ctctctagct cctttgacaa agtgatttga gatttatttg gttccgttgg
     1621 agtcaattct atagattctt cataaaaatc tctatacata aagctatcat taattttata
     1681 aaaaaaatta aatgttttta gcactgccat ataacattca aaacattctt ctatagttgg
     1741 tttacttcct tgaggagaat gaaccaattt attacgtttt tctatgaaat ttttaatttc
     1801 ctttcttatt tctttcggaa actcaatatt aaagtaccct ttaaacatgc tccctaataa
     1861 tttgtctaca gggggagatt gtaaattctc aaatattaga gttaaatcag gtttctcttg
     1921 ttgataaaat tcctttaccc ctatttcaca ggctgtaatt atcgaaataa acctatctct
     1981 agtatttgat atatttttac ttcttttaag gagtataaga gaaaagggga tactttcgtg
     2041 tccatgcatg taattaacta attgttgagc ttcagaagtt atatctctaa ttatatagtt
     2101 aacggaatta ggaagtgtct ttaactctaa tgaaatttct atccaattac tatcttctaa
     2161 tctgtatatg ctcgaagtat aatttgattc gtcagcaagc atcgctacat cccaaacact
     2221 gatcataaaa tcttttaaac gttcaatcat ttcagcaata ttgctaaaat cagctaattt
     2281 ttctttagaa taaaaaaaaa catttatatc agaatcaata aattctattt tttttattac
     2341 tatatcgtca tcattatctt gaccatttat gtttattgtt ccctgagtgg ggagtacttc
     2401 tttcaaattc aagttaaatt tatattccat tacttctcct ccaatatata aaaattactt
     2461 aactgtatcc aaatttaata tgtaaataaa tcattaattt ttagctaaat tctatgagaa
     2521 gtttattttt gttgtttttg ctaattaact aaaactagta caaaaaattt aaaatagatt
     2581 attaaaccta ttgcttattc tattcagtca tatatataag cgtcatcctt tagtaactaa
     2641 catcccacac tacaatagta ttataacata tctattcaac ttcaaattgt tgaaatttaa
     2701 gccatttata tttacttttc ctcaacttta gaaatgattt tactaacctt atatccgtaa
     2761 tacgcgtaac aaacacattc attaataaca atcaacacta agctatatta gctttttaaa
     2821 tgactagggt aaataaaata aggatcaaaa acttttaagt ttctgaccct ttccttactt
     2881 ccgattggtt aatagtgatc ttttgcagaa taaataaata tcgtttcatt ttcaactcta
     2941 tatatcagtc tatgttcatc tgtaattctt ctggaccatt ttccagataa atcatgcttt
     3001 aatggctcag gttttcctaa tccagcaaag ggggaacgat cgatatcttt tattaacttg
     3061 ttaatctttt ttatattgct tttgtttcct tgctcatgcc aataaagata atcatcccaa
     3121 gcatcatcag accaagcctt aatcatcaga ttcaacctcg attaagtcat gtgttttaaa
     3181 tgcacctttg gagaattgtt catctcctcg acgaattttt tccatgacgt aattattaga
     3241 aagtgttctc aacgtttctt gcatagaatc ataatctctt tttgataata caacaactgt
     3301 atcttctaca tctttacttg ttacaataag tgtttcagca tcctcattaa cttgtttcat
     3361 ataactacgt aaattttggc ggaaatttga ataagctact gcttccattc ctttcacccc
     3421 ttatttattt ctataaaaca attgtacatt atattgtaca attaagcaaa tgattaatat
     3481 tctaacttct tataattaaa agtgctatca cttttagttt ttaggataaa aagtgacgtc
     3541 actttttgag tgctggtacc tcaaatcctg ctttttcttg ggcaatcaat aaatctaacg
     3601 caatacacat aatcctggat tcacttttgc cgtacttctg ctttagttgc tgcaattctt
     3661 tttgtctctt ttcatccaac gtaacgggta accgtttatt tcctattgca attggcatta
     3721 gtactctctc ctttcatcga aataacccta tgaaaaatat ggctgcaatc atcttgaata
     3781 gtagactcac aagggctaat acaaagcgcc aaaagatcaa acaaaatacc agtattgcca
     3841 aaaatactac gaagtttaaa tgcacagctg cagcatggcg cataagtatt tcgccaacaa
     3901 ataggactaa caatagagga atgattttat tcatatggat ctccttcttt ctcttaggtt
     3961 atcgggtgtt gcccgcttat ttcgcaccac ctaagcggcg ggggcggttg gagcgattgg
     4021 tgcgaaataa gaaccattcc cttagttact aggttttttg tccttatttc acacattctc
     4081 acttttcggt ataaatgatt attgcctttt tttcctttca aatcgttgat atggcattcg
     4141 tttacggtca agttcatttt ggatttcttc tgtatcgtcg taagtatagg tgtctaaaaa
     4201 atcagttaaa gtcgtgtacc agtcgtcgcc tgggttatct acttcttcta catattccct
     4261 caaaaatgct tcgggtacag cgagttcata atccattccc attaacacga tagattccgt
     4321 tccacacgtc aaaaatctag taaatacaag gtaataatcg tctatcgtcg catctttgct
     4381 gtttagctct tccatttctt cttccattaa ttccatgctc tcaaaaattg aataatattc
     4441 tgattctgag taatagtgtt tcctgcaaat cgtgtagctg acaaagtttt cgaccagctc
     4501 actcctttct aggtaaacat tcatgatgta tttttctgtt tctagtcgta gaagctccgt
     4561 tccttttttt accgtttcag gacgacgttt atacacgtct aaacgattat ggctgactgt
     4621 gaccttcgtt tgactcgttt cactttccaa atacaattgt t
//
LOCUS       DACCCR010000130         4334 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCCR010000130 DACCCR010000000
VERSION     DACCCR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.143"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            128..565
                     /locus_tag="IVK93_002643"
     CDS             128..565
                     /locus_tag="IVK93_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387879.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            660..1544
                     /locus_tag="IVK93_002644"
     CDS             660..1544
                     /locus_tag="IVK93_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP6387880.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1766..>4334
                     /locus_tag="IVK93_002645"
     CDS             1766..>4334
                     /locus_tag="IVK93_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387881.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SETATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
BASE COUNT         1418 a          725 c          965 g         1226 t
ORIGIN      
        1 tattacttga tttagttctt tttattttaa aaattttttt tacttagaat ccagccaccc
       61 tagatatttt tctgtataat cttaaataaa gaaaacgata ttaaaaaatg cacgtagaag
      121 tagataaatg gagtggtttt gcaaatttct tttatttcta gaagttattc acgtatacgg
      181 atttgttttt ctcacaaata aaaaggcagg aagtacaatg gatcatttgt atataaaata
      241 tattcatcaa ttttccgttt tggtattagg cacactaatt atagcgggga ttgttgcatt
      301 tattattcaa ggtcgtgaca tattaacagt acatcaagag tttttcagtt atgcatttgg
      361 tattcctctg atcgctataa ggtctgataa caaaaagtgg aagaaacgct attacattct
      421 cttgctattt ttgtgttttt atctgccacc taagattttt actttattag gtatacaatg
      481 cctatcattt tcttttattt cctatatcat tgttttaatt ttcctcagtt tcatagtatg
      541 ggtattttat cttgtcaaaa aataaatgaa tgtttcatga acagtacttc atcacagcct
      601 aatttttttc tttatgctat cgttatatta aagaaatcaa tcagaaggga aagaaagtga
      661 tgaaaaaagt tttattagtg gtaaacccca gttcaggcgg tgaacaagca aaggaatttg
      721 agcaattagc gatagcaaaa ttggaatcgg tgtttgatga agtggtcgtc ttgcatacaa
      781 aaaaagcagg ggatgcaaaa aactttactc gcgaagcggc tacggaaggg tatcatagtg
      841 tgtttgtcat gggcggagat ggaacagtca acgaaggaat cagtggaatc gctgaacaag
      901 aacatcggcc aaatttcgga tttttcccat tgggtacggt gaatgacttg gctcgagcat
      961 tagggatacc gctagaacct gaggaagcga tcaatcattt ttcgattgaa tcagtcaagc
     1021 cattggatat tggaaaaata aatgacgact attttatgaa tgtcgtcgca attggctcta
     1081 tccctgaagc aatcaatgat gtagatgcgg agaagaagac gaaatttgga aaactagctt
     1141 atttcatgtc aggaatcaaa cagttagctt ccacacaatc ctactcgttt cacgtagaag
     1201 ttgatggaaa gaaggaagaa attgagagca gtatcttgct gataggacta actaactcag
     1261 taggcggttt tgaaacatta cttccaaatg caaaagtgga tgatggtaag ctccatttgg
     1321 tttatttaaa agattcttct ttgatcgata cgcttaaaac attgccagac ctattaaaag
     1381 gagtagatga atctacgaaa aatctagcct atcttacttg tgagcaatta gttgtgtcat
     1441 tagctgatga ggaagaattg acgacgaatg ttgatggaga tgaaggggat aaattacctg
     1501 tgacaatcaa agtgttaccc tcacatttac aggtttattg ttaaaatgga ggttgtgacc
     1561 gaagagttgt caggaggaca tcggttgttc ggaataaggg actgtgacaa gacttttgtc
     1621 acagcctttt tttgtacaaa aaagaaattt tcacctaaca gaaaaatttg ccataaaaat
     1681 acatatttta aatatgaaag tacaattaca aataaattaa aacgttaaaa taaaattgtc
     1741 aaaacgtcag agaggatggt ggagaatgaa tcaatatgta aaaaagctga ttgcgtcgat
     1801 tttgtttgtc ttccctctct tgatggtgac atccgttttt gccgatgtag agaaaacaag
     1861 cgaatcaacg caatcagaga atcaaagttt tgacaagact actttttcag aagaatcaat
     1921 tacttctgaa actacagaat cccaaagtga ggcgcctact tctaatacaa ggcaagaaga
     1981 aaataagaca agtgagacct cagaagtagc tgaacaaacg tctgatagca gtgaagaggt
     2041 acaggttaga gttgtttcag cggatgggta cagcgaaact gccacaacag aagaactgac
     2101 gcagcttctc gcagacgaat cagtagctaa gattcgttta actcagcctt tgactttaga
     2161 cagggaattg gagatcaagc gagatatcgt tatcgatttt ggcggacttg ctcataattt
     2221 cggcacacat cacatctata tcaatgagac acctcagtcg atcgagtttc agaattttaa
     2281 aggaacggca gctcatccag gtgggttgat accaaatatc gatggaaatg ccattatcat
     2341 tgcttatatg tccgattttt ggggaaacag atatcatttt acgggagaaa taaaatttac
     2401 cgggacactt gacttatatg atggttctaa attaggcctg atttacgccc ctcgtgcaac
     2461 tgtcacatta gatggtgttt ccggtgtatt ggatgtccaa ccagtgaaag aagggatgaa
     2521 tgccgctccg ggtaaagctt atttttgtag aagctatcag ctaaatgtaa tcaatggtag
     2581 tcagctttat ggaccatatc taggaaaatt ctatggtttt ctagattcag gtgatgagac
     2641 gggcagtgga aaaacagcac caggactcca tatcttgagt ggttcaaagg tctctcttga
     2701 ctatgatcgg gcagatggcg tcagcgatga aggagaggct gtcgatacgc tacccgggaa
     2761 tgtcacattt aaagtcagtg gctcaggaag tgaattttcg gtaaatacaa agatcaatat
     2821 taccaacgac aacaatcgcg gaattatcca aatgagaggc tcaggatcaa gagtcgctgt
     2881 gtcgaatgat gggaaaatca caatcaatac agcgactact ggcggatttc gcctgcaagg
     2941 agattctagt caaatccatg tatcaagtgg tggacaaatc tcggtcaaaa tggctggaga
     3001 tggcgaccaa cctggaaaca atgggatgcg ttttgtaggg aaaggacttt ctttgatcgt
     3061 agaaggagcc ggatcagtca tcgatattga taagcaaagt ggtcgttcta gcgcagttcg
     3121 ttttgagaac ggtacgcaaa aactaacagt aactaatgga ggctcattga aggtaagaaa
     3181 tgcaggggat ggcaaatctt atgtgaacag actcaatcaa ggaaaccaag cgatccaatt
     3241 ttatgacgct tctggttttc tagaaagtcc agggtcagct gattttacgg ttactggaga
     3301 aaaatcaaat atcgatatac gcgcagattc cggagctacc gtagatacaa ctggagatat
     3361 tgatttgaac tttatggctg gcgaaaaaac ttatttagtc atggtcggga aaactgggga
     3421 agataagact ggcatcatca gtggggatgt gttgaatgta gacttagaat ctcctaccta
     3481 tttcgatttc caaaacaaac ggatcggaag cgagactaat ggcataggtg gctgggtatt
     3541 tcaaggaaat ggcgattctt ctttgaagat cgctcattca gaaattgctt tatggcgaaa
     3601 ggcaggagta acgttcgata actttgaaaa agatccggat tatttttctg atatgacaga
     3661 cttatcgatc aatggaaccg acttaggtaa ttttgtatca agccctagtg caggtttaac
     3721 aaatgaattt gctcagacag gtgccggaat gaaaaattat aatcgaatca gtgccaataa
     3781 ccagcttccg atgattgaat ccttaagagt accaacgaat gcagataaaa aaatctatgg
     3841 gcatgtcgtt gttcccgaag gtgtcgaggc aaagccaaga gatgcttgga caggcgaagt
     3901 agaagtgact ttacgtatta cttatgcaga tacgacgaaa tcacctgtag aaattactgc
     3961 tctcacacaa ggacaaacag atgaaaatga agggacagga tataatccgt ggggagaagg
     4021 cgaacaaggc ggtttgtttg aggttcaagt acccaacgaa gaaagactaa caacggggga
     4081 tcaagtgcaa atagttgctg ctaaaaaagt aagaggcggc caagaagtga cagaactgga
     4141 ctctgccaaa acgacagtcg atgtgattcc tcctgagcct gctaaattaa gtaccacatt
     4201 gatcaatgca gcttctcgct caatcacagg tacaggcgaa gcaggagctt tggtttcatt
     4261 gaaaaaaaat caagctgttt tgtccgaaac aactattgat gatacaggta aatttgttct
     4321 ttctatccca gaaa
//
LOCUS       DACCCR010000131         4234 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCCR010000131 DACCCR010000000
VERSION     DACCCR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.144"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(37..1257)
                     /locus_tag="IVK93_002646"
     CDS             complement(37..1257)
                     /locus_tag="IVK93_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6387882.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IVK93_002647"
     CDS             complement(1277..1882)
                     /locus_tag="IVK93_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6387883.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IVK93_002648"
     CDS             complement(1931..2863)
                     /locus_tag="IVK93_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP6387884.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IVK93_002649"
     CDS             complement(3284..4144)
                     /locus_tag="IVK93_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387885.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1204 a          853 c          694 g         1483 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgagaatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aattggttaa gata
//
LOCUS       DACCCR010000132         4157 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCCR010000132 DACCCR010000000
VERSION     DACCCR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.145"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            179..775
                     /locus_tag="IVK93_002650"
     CDS             179..775
                     /locus_tag="IVK93_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6387886.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IVK93_002651"
     CDS             899..2698
                     /locus_tag="IVK93_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6387887.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IVK93_002652"
     CDS             complement(2976..3188)
                     /locus_tag="IVK93_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387888.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4052..>4157)
                     /locus_tag="IVK93_002653"
     CDS             complement(4052..>4157)
                     /locus_tag="IVK93_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6387889.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          707 c          729 g         1493 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DACCCR010000133         4005 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCCR010000133 DACCCR010000000
VERSION     DACCCR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.147"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            124..789
                     /locus_tag="IVK93_002654"
                     /pseudo
     CDS             124..789
                     /locus_tag="IVK93_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            825..1070
                     /locus_tag="IVK93_002655"
     CDS             825..1070
                     /locus_tag="IVK93_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6387890.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1173..2042
                     /locus_tag="IVK93_002656"
     CDS             1173..2042
                     /locus_tag="IVK93_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP6387891.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2023..2757
                     /locus_tag="IVK93_002657"
     CDS             2023..2757
                     /locus_tag="IVK93_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6387892.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2790..3698
                     /locus_tag="IVK93_002658"
     CDS             2790..3698
                     /locus_tag="IVK93_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP6387893.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3695..>4005
                     /locus_tag="IVK93_002659"
     CDS             3695..>4005
                     /locus_tag="IVK93_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387894.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AV"
BASE COUNT         1398 a          570 c          851 g         1186 t
ORIGIN      
        1 agaggtcttt ccatttagat agtagtggaa agacctctat ttatcttttg aaagtgaata
       61 caaaaataaa gcacactctg ctataatata ggtgattaat ccatttgaga gaggtgcttt
      121 atcatgtatc aaaattatac cataaaccaa ctttgtttgc ctatagatct tgaaattaaa
      181 ttagaagaaa atgattttgc tcatgccatt gtgcagtttg ttgactctat tcccgatgaa
      241 gtctttcttc cttattatca atctatgggt agaccccaat atcatcctcg tatgctactt
      301 tccattattc tttgtgccta tattcaaggg gtttattcgg gccgccaaat acaaaatatg
      361 ctgatagata gtattcggat gcgctactta tctcaagagc aattccctaa ctttcgtact
      421 attaatcgtt ttcgcgttca tccgattatg aacgatattc tagatcattc atttgttcaa
      481 tttcgtgagc tactggttca aagtggctta atcagtggca gtgctctata cattgatggt
      541 acaaaaattg aagcggatgc gaataaatac tctttcgttt ggaaaaaaag tattttaaag
      601 tataaggata gccttgatca aaaagccctt gaaaactacc aacaaatggt ggaaacaaaa
      661 atcttacctg agttaataga tgatttaaag gatgaacttt ctatagaaga gattgaaagg
      721 attcgtcaat ctttggaaga aaaggaaaat caattaattg aagcgattga ccaaaccgaa
      781 acagtagaag ggggctgacc cattattaat aaggagtgga caacatgatt gatgtaaatg
      841 agctattgga agaagctatt agagaaactg aaaatctaaa tgatggcgaa gtttttcttg
      901 ttaaagattt attcaaggga tatgtgggga ataggatacc cagaaaggat cgacttctgc
      961 ttggaacgtt atttttaaac catgttagca aaatgaatgg taatttaaag gctattgaaa
     1021 agacttcatc taatcagcag aggtataaaa agacaattga taagttttaa ctatcttttg
     1081 tgaaattaaa aaaattataa atatgttgag tagaagcaaa agatttggct tggaattttt
     1141 acagcagtga atcgataaaa ggagcatgct tgatggtaga taatattatt aaatcagtag
     1201 cagagaaatt atcctctctg tcttatatag aaggtattgt tttaggtggt tcacgtgcaa
     1261 ggggcaccca tacagaggat tcggatatag atatcggcat ctattacaat tcagaatcat
     1321 ttgacataaa tactattaat caattcgcta caaagctgga tgatgagcat agaaataacc
     1381 ttgttgtacc tcccggagca tggggtgatt ggattaatgg cggcggatgg ttagtcataa
     1441 acgggtatca tgtggattta attttacgtg atattaaacg tgtggaacaa ataatgaaag
     1501 atacagagca cggaattgtt actgccaatt atcagactgg gcatccccat ggttatatta
     1561 gtgcaatgta tcgaggagaa ttagcgatta gcaaaatact atatgctaag aatgaaagct
     1621 tatgcgaatt aaaaaaacag gcagaaactt atcccaatgc tttgcagaaa agtttagtta
     1681 acttttttat gtttgaagca gggttctctt taatgtttgt aaaagcaaat tcgggaacag
     1741 acgataaata ttatattgcg ggtcatgttt ttcgtatagt ttcatgttta aatcaagtgt
     1801 tatttgcatg taataatgct tattgtatca acgaaaagaa agctataaaa ctgcttgaaa
     1861 cttttgaaca taaacctgaa aaatataccg agaaggtaaa tcatattttt gaagtactcg
     1921 gtatctcact ttttgaatgc tacgacatga ccgagaagct ttataatgaa gtgaatgaaa
     1981 ttgtatcgga gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat
     2041 gatgataata tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct
     2101 ggtgcgggag aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg
     2161 cttgatttag gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc
     2221 tctgtagtag gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat
     2281 tttccacaga ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc
     2341 tttgatgtaa tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata
     2401 aaaaagatat ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct
     2461 gtttttactg ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat
     2521 ttcccggtgg acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag
     2581 gttacaaaat atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt
     2641 ataataaatc agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg
     2701 gcggatgaaa tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa
     2761 tatagaaaaa ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag
     2821 tactttcttt agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg
     2881 caaatattaa tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg
     2941 atatagaacc gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga
     3001 tgcaaaagcc ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta
     3061 tgctatttga tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata
     3121 attacctaaa gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa
     3181 gggacatagt tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg
     3241 atgattgctg caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta
     3301 aggaaatttt atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa
     3361 tgatatcatg gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata
     3421 agtttattga aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga
     3481 tggattccta tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg
     3541 tatccggtga ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa
     3601 caaaatatac cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca
     3661 catatgccgc tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca
     3721 cctgaaagat atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag
     3781 gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag
     3841 ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa
     3901 tactgataag ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact
     3961 gcgaaaaaat tggaaccggt acgcttatat agaagatatc gccgt
//
LOCUS       DACCCR010000134         3923 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCCR010000134 DACCCR010000000
VERSION     DACCCR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.148"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(573..1130)
                     /locus_tag="IVK93_002660"
     CDS             complement(573..1130)
                     /locus_tag="IVK93_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387895.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IVK93_002661"
     CDS             complement(1309..1920)
                     /locus_tag="IVK93_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387896.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IVK93_002662"
     CDS             complement(2033..2290)
                     /locus_tag="IVK93_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387897.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IVK93_002663"
     CDS             complement(2887..3165)
                     /locus_tag="IVK93_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387898.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IVK93_002664"
     CDS             complement(3199..3450)
                     /locus_tag="IVK93_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387899.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DACCCR010000135         3879 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCCR010000135 DACCCR010000000
VERSION     DACCCR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.149"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..85)
                     /locus_tag="IVK93_002665"
     CDS             complement(<1..85)
                     /locus_tag="IVK93_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6387900.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            161..316
                     /locus_tag="IVK93_002666"
                     /pseudo
     CDS             161..316
                     /locus_tag="IVK93_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            335..1612
                     /locus_tag="IVK93_002667"
     CDS             335..1612
                     /locus_tag="IVK93_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387901.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="IVK93_002668"
     CDS             1622..2278
                     /locus_tag="IVK93_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6387902.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="IVK93_002669"
     CDS             2278..3654
                     /locus_tag="IVK93_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6387903.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
BASE COUNT         1547 a          413 c          676 g         1243 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgtt
//
LOCUS       DACCCR010000136         3785 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCCR010000136 DACCCR010000000
VERSION     DACCCR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.150"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..171
                     /locus_tag="IVK93_002670"
     CDS             <1..171
                     /locus_tag="IVK93_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6387904.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            346..642
                     /locus_tag="IVK93_002671"
     CDS             346..642
                     /locus_tag="IVK93_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387905.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="IVK93_002672"
     CDS             649..2601
                     /locus_tag="IVK93_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP6387906.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IVK93_002673"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IVK93_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP6387907.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="IVK93_002674"
     CDS             3400..>3785
                     /locus_tag="IVK93_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP6387908.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DACCCR010000137         3682 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCCR010000137 DACCCR010000000
VERSION     DACCCR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.151"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..278
                     /locus_tag="IVK93_002675"
     CDS             <1..278
                     /locus_tag="IVK93_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP6387909.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            421..642
                     /locus_tag="IVK93_002676"
     CDS             421..642
                     /locus_tag="IVK93_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6387910.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            658..1500
                     /locus_tag="IVK93_002677"
     CDS             658..1500
                     /locus_tag="IVK93_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387911.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1563..3365
                     /locus_tag="IVK93_002678"
     CDS             1563..3365
                     /locus_tag="IVK93_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP6387912.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRVESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3424..>3682)
                     /locus_tag="IVK93_002679"
     CDS             complement(3424..>3682)
                     /locus_tag="IVK93_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP6387913.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1313 a          520 c          750 g         1099 t
ORIGIN      
        1 aagaactcat caagctggct aataagggtc aagattatga acatctagca gatgagattg
       61 atgagctgag agacaagcga cagctccttt tagtagaaga tgcctccctc agtggcgaga
      121 acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgt accttagagt
      181 acgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa gaacacttcg
      241 tcatatcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta aatagcctat
      301 gattctacag tagagtcgtg ggttttcttt tgcttgctga ttataataat attttaataa
      361 ctattgttat tataaaccaa atgatttata atattcttag cgtaaacttg gaggtgctgt
      421 atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag tgtttccgaa
      481 cttgctagac gtattggaca gactccacag aatttcaata agaaattaca acgagaaacg
      541 gtaaccttag atgagttgaa agcgattgct gatgtgctag gtgtcaagtt tgtgcaggct
      601 tttatattgc ctgatggcaa tgaaataaag ataggtaatt aggaggtgta atttgtattg
      661 aaaatcataa caataaatga tgttgagtat gctgtatttg ctgctaatga aggtacttcc
      721 aaaccacaac ctcatatcat agaaacgaaa tctggtacta ttccagaagg aaaacaactg
      781 agtttattaa aagaatatct taagcagaat gatatttccc caataaaagg agcaacaaca
      841 tattggtgca ttgacaaggt tcttaagctt gattcaagta aagaaaaaac tataagtgaa
      901 acaatccata aacaaaaata cttatcttta actgaagaaa atattgagaa acagcataag
      961 tttgtaggtg caagctctaa ttatgggaaa gaaggtctaa taatacatga tgttttgaat
     1021 gcatttcctc ttcataatga tttaaatact attgcgatga agattgcggt aattgatgta
     1081 acaaattcaa cacatctttc tcaatataag agcaggttgt ctctatatga tttggctaaa
     1141 gtaattctgg agattccaaa ttttgatgat cggttggcaa aaggagatcc tcagttgata
     1201 aatattatcg caaggaacat tggtgcagtt aatatgttct catttgcatc caagtactgt
     1261 acatatcata atgtggaagt gtgtgggaga gatgattact ccatctttga tggtatcgtt
     1321 aaaaatactc ttccacacta tatccaagga ctaactacga ataagattga tacgtggcga
     1381 agaagttttg attacgaggc atttaacgaa tgtgttggaa aactacttga tgaaaataat
     1441 atacatattc cttttagaag aagaaagtta gatcacttct tgtggtatgc gaataggtag
     1501 aatataagaa aaaggcatcg taaaagtgat tctggataaa attaatgggg gaggaaataa
     1561 tagtgggatt atttgatttt ctaaagaaaa aacaaggaaa ggttagtaaa cacgttgatt
     1621 atcaactggc ttctagtcta tttcgtactg gtaaagtttt tgctgaaaaa ttggaagtgg
     1681 acatatcaaa tgtagatgag tttcgtaaaa cttttatcgc ttttgatgtg gaaacaactg
     1741 gcctaaattc ttcttcagat agaattgtag agattggtgc ggtgctattt aaagaaggag
     1801 ttccaattca atcattcgga actcttgtca atccaaaagt tagaattgca gcctcagctt
     1861 cggcaataaa taatataact aatgaaatgc taaactctgc tccaaatgag caggaagttt
     1921 acccccagtt ggtagaattt ttaggagatg cactagatgg aaaaatcact atgtgtgcac
     1981 acaatgcaag tttcgatttt ggatttttaa gtaacacatt ttctagactt gggtatgatg
     2041 ctaaaattaa gtatgttgat actctgagtc tctctagaaa acatataaaa ggcattgtaa
     2101 atcataagca agagactgta gcggaatatt tcggattaat aaatgacact gcccatagag
     2161 tggaatcaga tgcagatata tgcggaaaga ttttttgggg tgttttagat attattgagg
     2221 agtcgttgga agaacaaaaa aagcaaatag aaaagatgac tccagacata aaagaacttg
     2281 aagcttgtgc ttttattcaa gatattattg agaaaaaagg atgcgacacc agttggatca
     2341 ggtatcgaaa aaacagtaat aattatgtcg atgtaacttg tctatataca ttcttaaagt
     2401 ttaagtttgc taagaaggga aattacatca ttattagaaa agatgctaca aatggaattg
     2461 atttacctat agaaccttgt actgtaagtg aaggcggtac aacaaatgtg cgagtctatt
     2521 ttcgtagccc ctttgactta acgcctcttt caaattatat tttcagtgct tatagcgatt
     2581 gttataattc tatgaatgaa tatatgagtg ttagtaatta cactaaaaaa gaagcagagc
     2641 aaagccttag tatgctgaaa tctatttcaa aatcagcgat gaaagagtta ttattcgatg
     2701 caaaaaatcg tggatatgat gagactgaag ttaacctaaa tattgaaaaa gtgatatcta
     2761 gagaagatgt aattattaac gggttgcata atcgagtttc attaaacaag atacgaaata
     2821 tagggaattg ggagaaagga tttgatgcag gttatccata ttgggaacgt ggagaaactg
     2881 ctaggaaaaa tggcaaagta gatgaagcta ttgcactttt tgataaagct cgatataacg
     2941 gctatgatgc tcctgcgcta tatgattctt atgctaaagc ttatcgaaaa attaaggatt
     3001 acgataatga aattctcatt cttgatgaag gaattatgcg taaaacacgg cacgatgttg
     3061 gtactttaga ggctcgtagg gataaggcaa ttaagctatt atttgctaaa caagaggcag
     3121 agcggatagc taaggaaaaa tctgattttc taaaagcgaa caaaaaagag gatgcgatta
     3181 gtgagcctag caagaagcgt ggaagacaga ttattcaaat gactgatgat ggaacaataa
     3241 taaaagaatt cgatactata gcttctgctg taaaagaagt tggcgttaac tcaaaaagta
     3301 ttagggatgc tgcaaatgga gttcaaaaac atgctggagg atactactgg aaatacaaag
     3361 attagcagat aatctaagat aacctaggat cttcttaata gatacccgtg gtgctagtta
     3421 attttaatat cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa
     3481 tagaacactg taagccagta atatgctttc aaatctactc tctagccctt taacagaacg
     3541 tgagttgaaa ttttgacaac ctaatttttc taaggaagaa aaaacagtcc caactgtttt
     3601 tctttgtttt ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg
     3661 cggaatggat atagtaatgc cc
//
LOCUS       DACCCR010000138         3524 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCCR010000138 DACCCR010000000
VERSION     DACCCR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.152"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..411
                     /locus_tag="IVK93_002680"
     CDS             <1..411
                     /locus_tag="IVK93_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387914.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="IVK93_002681"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="IVK93_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP6387915.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..>3524
                     /locus_tag="IVK93_002682"
     CDS             2446..>3524
                     /locus_tag="IVK93_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAP6387916.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFL"
BASE COUNT         1159 a          510 c          723 g         1132 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaa
//
LOCUS       DACCCR010000139         3298 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCCR010000139 DACCCR010000000
VERSION     DACCCR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.153"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            138..1106
                     /locus_tag="IVK93_002683"
     CDS             138..1106
                     /locus_tag="IVK93_002683"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP6387917.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1423..1947)
                     /locus_tag="IVK93_002684"
     CDS             complement(1423..1947)
                     /locus_tag="IVK93_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP6387918.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2234..>3298
                     /gene="scm"
                     /locus_tag="IVK93_002685"
     CDS             2234..>3298
                     /gene="scm"
                     /locus_tag="IVK93_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP6387919.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         1052 a          667 c          611 g          968 t
ORIGIN      
        1 aaaaatgaac gcccaccctc ttcttttttc tctttccttc tttcgttttt gctttatgta
       61 tcaaccgttg tttcttcgaa aactattttg ttctctcctt cttcctgata gaatggatgg
      121 gaaaggagct tgatgccatg acaacgaaag acaaggtact tcaattattg aaaaagacac
      181 cagattttct ttccggagaa aagttggcac agcaactgga agtttcccgg acaagtattt
      241 ggaaagccat caaagaattg gaaaaggcag gctatcgttt tgagcacggg cctacagggt
      301 atcgttacct gccctctgac gtactagatg caaaagaaat ccagcaagag ctacggtcgt
      361 ttattcctga tttgaaagta caaacgatgt tgacttctga atctacgatg aaagatgcca
      421 aactggcagc agctcaagga gattctgttt cgaagttatt tgttgctgat acacaagaac
      481 aagctcgtgg acgttttgga cggccttttt tcgctgaacc tggacgagga atctacatga
      541 gtctcttgct ccatccagac aaacggtttg atgaattgcc tcaatatacg atcatcgcag
      601 ccagtgcctg tgtgaaagcc attgaatctg taaccggtcg ttctgcttcg atcaaatggg
      661 tcaatgatat ctacatggat ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg
      721 atatggaaag cggcaggatc agtcatatca tcattgggat cgggcttaat ttttctgttc
      781 ctcaaaatca ttttccagct gatctccaag aaaaagccat ctctgtcttt cccgacggac
      841 aagctagtgt cacaaggaat cagctaatca gcaaaatctg gcaagaattg ttcacttatc
      901 tttctcaccc agaaaaagcg ttggacttct ataaagaaaa atcattcgta ttgggaaaaa
      961 ccgtgtcgtt caatcaaaaa ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc
     1021 atggcgaact gatcgtccaa acaaatgaac agaagatgag tttgtcttct ggcgaaatca
     1081 gtctcacatc tatccgtacg caataattat cagatttcac aaaaggacac ctgatctcat
     1141 acagtgtcct tttttctgaa ttctcttcaa aagactctcc ttattctatc agctgacagt
     1201 ttctctatcc tctagaaaaa ggtattctaa aaatatctat aaagcatggt acactttctt
     1261 taaatgaatc aatgcaaaaa acaagagagt catcagttta cacgatcaaa agagatagca
     1321 ggccgcttcc tttttcaatc ttatagatag accatgggta acgataaaaa caaaaaagaa
     1381 cttgcgactt attttgccgt aagttctttt ttagctagtg ccttaatctc ttaatcccaa
     1441 caaacgtctc gcatagatca cctgaccaga gtgcatagca gcatcatcaa taatagaaac
     1501 gagacgcatt cctcgtgtga ctggtggtgt ccagttctcg tcaattactt catctagctc
     1561 ggaaggttcc agccttctta agtaagaaat agcaaaatca gcagctcctc gcaagtatcc
     1621 tagcagatct tctatttgat tagcttggat ctgctttgct tgttccagac tatgcttcca
     1681 atcttgttcg ttatctgcta tcgtcatcgg aaaccggctt tcccagtctt gactatgcca
     1741 aattggagaa gttcctgcta aatcagcaat ctggaaatct aattctcgag cggtatgcca
     1801 agccagccaa gtcattgatt tgatctgcgg ggctctatct tccactggaa aacgattggc
     1861 ctcctcaata gagacgcctt caaacaaacg ttcaaaccga tcgtatgccc gatccacact
     1921 ctcgcatagc atatctaaat aatccattta ttttcctcct ttttatttat cctacttact
     1981 ttttcctaat tctaggatac ctacttatat gttactacac acccttattt tttacacttt
     2041 aacagtcacc atcatcaata gtttggagat tgaaagaaaa agatagtgtc gaactatcaa
     2101 ttgaacaaga agcaacaata tgaacgtttc attaatattc tttaaataaa caatgatttt
     2161 tcaaacgaac agtttaagac taggagaaaa agagtataat aggaaaatgt ttgaaaggaa
     2221 aggaagggga aaaatgaaaa aaagggcaag tttactagtc ctagttgctt tgttgttcaa
     2281 tatcttttcg tatggtgtgg ccgtttcagc ggaaacatac attcctaaag accctgggaa
     2341 tatttataat gatctaactg gtaactatgg cttgttagga attgctagtc aattccacgt
     2401 atttgctaaa aataagacaa ctgtgaatgc acatacagac ggaaatatag caacacatga
     2461 acttgatgca aaaaataatt ttggtacaga gcttcatgaa ggtttattga accaagaaat
     2521 aaattatata caaaaaactg attctttcgc taattcttca ggcataccta caggtagcac
     2581 aagaatgaat aaatttgtcg ttggcagtaa cgtggatgta gcacttcaag atggtcatcc
     2641 tacagtcaat ggatccaggc ttgatcactt aactccagat gatttttata aagatcgtgc
     2701 tgggcatgaa tatattgatt tcgagcagga atttgctaaa ttgaatgtag cagcaaatga
     2761 tcttgcaacg atcactccag ctaaaacata tactgctgca gattttccag acatgaacaa
     2821 tcggacaatc gatttaactg atttatcaga taccgggttt ttattggtaa atatcgatgc
     2881 agaagtgtta acgatgaata cgccacttca aatcattaat ccaaatgatc aagtagttgt
     2941 atttaatgtc atcaacagtt cttctgcatt gaacgtacag tcaccaatca aatacaatga
     3001 tcgttcaaat catgaaacag aagatttttc ggatgcgaat atctcttgga attttggaaa
     3061 tgagatgact gatttgacaa tcagtgctcc ttttcaagga acaatccttg cgcctaatgc
     3121 tactatccgc attactcaaa accaagacgg aacgattata ggaacaaatg tgattttaga
     3181 tgcagctacc aatcgttggg atcctaacga aatatttatc agcgacaatg gtacagatac
     3241 gacagacaca agcgacagta cagatacatc taccacaagt gatagctcag attcatca
//
LOCUS       DACCCR010000140         3280 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCCR010000140 DACCCR010000000
VERSION     DACCCR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.154"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..584
                     /locus_tag="IVK93_002686"
     CDS             <1..584
                     /locus_tag="IVK93_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6387920.1"
                     /translation="AFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELS
                     KKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMAN
                     SGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVL
                     EGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(661..1836)
                     /locus_tag="IVK93_002687"
     CDS             complement(661..1836)
                     /locus_tag="IVK93_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6387921.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1918..2298
                     /locus_tag="IVK93_002688"
     CDS             1918..2298
                     /locus_tag="IVK93_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP6387922.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2372..2734
                     /locus_tag="IVK93_002689"
     CDS             2372..2734
                     /locus_tag="IVK93_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387923.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1113 a          538 c          622 g         1007 t
ORIGIN      
        1 tggcattccc acaactagat ttaaaaagtg aaaaaggacc aaaagctgtt atcaaaacaa
       61 accgtggtga cattactgtt caattattcc ctgaactagc accgaagacg gttcaaaatt
      121 tcattgaact ttccaaaaaa ggttattatg atggcgtgat ttttcaccgc gttattcctg
      181 atttcatgat ccaaggcggc gatccaactg gaacaggaat gggtggcgaa agtatttatg
      241 gcgaaagttt tgaagatgaa ttcagtcgtg aactgttcaa ccttcgcgga gctttatcta
      301 tggcaaattc aggtccaaac acaaacggaa gccaattctt tattgtaaat aacacaaatg
      361 tgccagctaa tatgcttgga caactagaag gtgctggatt cccatcagaa atcattgaag
      421 cgtataaagg tggcggaact ccttggctag atttccgaca tacagtcttt ggacatgtac
      481 tagaagggat ggacacagta gatgaaatcg caaatgtaca acgtggtcca caagaccgac
      541 ctgtacatga tgtagtgatt gaaacaattg aaatttcaga atagtttggt atggtacgga
      601 gtaattatga aaaaggacga aacctagtaa ggtctcgtcc ttttattttg aataaatgat
      661 ttaatcgatt gtctctaaag catacttgat tcgattaagt ccttccagta aagtttcttc
      721 tggacaagca atattcaagc gcatatgccc atgaccggaa ggaccaaacg tgatgccagg
      781 atttaatacg acttttcctt tccgaacaag agtgttctcc aattcttggt cgtctgaaat
      841 ataggcggta aaatctaacc acattaagta agttccttca gggttcatta ttctcacttt
      901 cggaagatac tgttcaaaaa actgattaac tgtttgaaca tttttttcta gataaggaat
      961 cagttggctt aaccattctc cacccgtctc ataagccgct tgggttccaa tcagtccaaa
     1021 agtgttgatt tcgtgctgtt ggttcatcac taaatgtttt tcaaaatcag ctttcatctc
     1081 tggatttttg ataaatacca ttgaattttt gattgcagct agattgaaag tcttggttgc
     1141 ggaagtaaaa gcaactagta gatcatctaa attaggatca atcgttagca tggaagtaaa
     1201 tgtatgatcg aataaagtta aatcttgatg gatctcgtca ctaaacagaa tcacttgatg
     1261 ttttaaacac aattcgctta actgtttcaa ttcttctttg ctccaaacac gccctccagg
     1321 attgtgagga ttgcataaaa tcattgcttt tacattgttt tcttcgattt ttttctccat
     1381 gtcagacaag tccatgatga aatgattatt tttttctagc aaagagcttc gaacgatgtg
     1441 acgttgattt gtttctataa tagaggaaaa tggaggataa actgggtcat gaatcaaaat
     1501 agaatcgcct ggttttgtaa aagtttgtac tgctgcagct aaactggtta acacaccact
     1561 tgtaaaaacg atattttctt tcgtcaactc cacagcatgg tgttggcgtt cccaatgaat
     1621 gatagcctga tatagtttat cagacagagt ggcataacca aagattccat gtttgatgta
     1681 ttcactaaac gctttagcta ccccaccggg agacaagaag tccatatctg ctacccacaa
     1741 aggaagtaag tcatccattt gatatgtctc agcaattgcg tcccatttta cactttctgt
     1801 atcttttctt gaaatctgtt tatcaaattc gatcatctta agacctccag tcattttcgt
     1861 cgcttctttt tttgagctat ttacagtata atgataaatg cgaagagagg gaagtagatg
     1921 acttataaaa ttggacaaat aattgaagga aaagtgacag ggatacagcc ttatggcgca
     1981 ttcgtttctc ttgataacga gacgcaagga cttattcatg tttcagaagt acaatccggt
     2041 tatacgaaaa atatccattc attgttaaaa gtcggacagc cagtaacagt tcagatcatc
     2101 gacattgatg aatattccaa aaaaatcagt ttatctttaa gaacgttaga aaaaacaaat
     2161 ccagctgttc catatcgcag aaaaaggtat ttcacgaata aaaatcgtaa gatcggattt
     2221 accactattg cggagcagtt acctatttgg atagaagaag cattatctga actagaacgg
     2281 aaagaaaata agaaataaaa agtgtatttt ttctgaaaat gtgctagaat ttttatgtaa
     2341 gccatagaac aagttgatcg aggtggaaat tttggcaagc aaaagagttg ctggtacaat
     2401 tatgttgcat ttggaggatg gtactaaaaa atttttggtc cattcaattg atgacaagtt
     2461 ggaatttgcg ttagcggaac tttctgaagg aaagactggt ttagcgaata tacttaattt
     2521 cttaaaagaa atagttcata tcgatgttag caagattagt ttgatggaat tgacaaatac
     2581 acatattaaa gaggaaaatg ttccgttgtt tgtctttgaa actgaacaaa agaatcaaag
     2641 agaagaatta ggcgaaggat atatttggga agaaccaggt caaatgcgct ctgttcttgg
     2701 aacttacgaa gtagagggag tgcctttctt ctaaaataca gattaagcga acaaagaagt
     2761 ttgaattttt ttcaaacttc tttttttatt gtttggtcca aaaaatacaa tgaaatgttc
     2821 tgctaaagat agttttttag tgcattttat tttcaaaata tgttgaaaac ggttgtataa
     2881 cgaataataa taattgatcg aaaaatcatt tcacaaagta atgcttaatt tatagttgac
     2941 caaggaagaa atacatgata aatttaacag cgttgattct gaagtggatg ttatatcaca
     3001 tttctaaaaa atttaaaaaa tagttattga catgttacta acgacatgat aagataactg
     3061 agtcgttaaa aaagaaccac ttgcgaagct tgattctatc gaaaaaaact tcaaaaaagt
     3121 tcttgacaaa taacaaacga taagttatga taaatgagtt gctgaaagat ttcgaaaaac
     3181 gaaatcgaaa aaaactttta aaaaagagtt gacaatcgct tgtctctctg atatgatata
     3241 aaagttgcta cggcagccaa aaacgaatcg ggaaacactc
//
LOCUS       DACCCR010000141         2947 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCCR010000141 DACCCR010000000
VERSION     DACCCR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.155"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            172..1476
                     /locus_tag="IVK93_002690"
     CDS             172..1476
                     /locus_tag="IVK93_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6387924.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1572..2441
                     /locus_tag="IVK93_002691"
     CDS             1572..2441
                     /locus_tag="IVK93_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6387925.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2553..2873)
                     /locus_tag="IVK93_002692"
     CDS             complement(2553..2873)
                     /locus_tag="IVK93_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP6387926.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
BASE COUNT          974 a          557 c          596 g          820 t
ORIGIN      
        1 tgaagatgaa aaattgaaaa aaagacaaga attacttgaa gaagtgtata cggaacgttt
       61 tggaacaaaa gaagagagag aaacggtccg tttttattct gtctccgaag agaaaaatct
      121 agatacgaca ttcattgcag atttgtataa agaaaatggg gtggaactga aatgatctca
      181 atcacccata caggtaattt attcttagat acttgtttgt ctattatgta tttttttctc
      241 gtttcttatc cgattttagg cggatttgta tggtttattg gtgtctggtg ttatgtgttt
      301 ttatacaaac ataagcaaaa agaatgggta gatgttccct tgagtgtcga accgttcatc
      361 acgatcatgg tgccagccca taatgaagaa attgtgatcg aagatacaat cgagtatttg
      421 atgacgaaat tgaattatca caactatgag gtattagtga cagacgatgg ttcaacggac
      481 caaacaccag aaatattagc tagactgatg aaaaaatacg ctaatcttcg tgtggttcgg
      541 atcgaaaaaa ataaaggaaa agcccacgcg tttaatatcg gacttgcctt tgcaaaaggg
      601 aaattgattt taagcaatga tgcagacacg gtaccggagc cagatgcact gattaggtat
      661 gtgaattatt tcattcgccc tggtgcaaga catattgctg cggtcactgc aaatatggat
      721 gtccaaaatc gtaccaaatt gattgcaaaa tctcaaacgg ttgaattctc aagtatcgtg
      781 ggcatcatta aacgaacaca gtcagctgtt ttcggtggtc tttatgcgta cagtggagca
      841 aatacgatgt atcgaaaaga agccttgatc gatgtgggcg gattcagaca agatcgagcg
      901 acagaagaca tcagtattgc ttgggatcat cagttaaatg actggttatc tgtttttgct
      961 cctgagatta tctttttcat ggaagtacct gtgacgttga agatgctata tcgacacaga
     1021 aaacgttggg caaaaggtgg aacagaagtc tggctgacga atttcaaaaa agtgatgctc
     1081 catcctttta aacatattgg aagaacgatc atcttcattg atcaaacatt gagtattgtc
     1141 tggtcaatat tcttttgtat ttccgtagtt ctttttgctg gattaatcgg gcattatgta
     1201 tatcaaggaa actacgaaca gatttatatt acttttacat tcagttttgt gttcatctgc
     1261 tttgaaatgg tcgcaggatt tttccagttg ctagcttcgc tgattgtaga tgatcgacga
     1321 agaaaattga aataccttct tttcgcacca ctatatatgc ttttgttctg gatagtgaac
     1381 gcgataacga tcgtgacgac tttcattcca gcagtcaaaa cgattttagg ttatggaagc
     1441 gggacatgga aaagcccgga acgtacaaaa aaataaaaga tgcaccgttt tttagacaaa
     1501 cagcctgttg tgtttatggt tcttccaaac atttttgctt ataataagaa attagttaaa
     1561 aagtagtaag gatgggaaaa gtgatgaaaa aaagaatgaa gccgttaatc tggccaattg
     1621 ctgtttccag tattttctta ttgggacaga cagcttttgc ggaaacttct gaacctgctc
     1681 aaacagaacc ttcaacaacc ataagctctt ctggtgctga aaacggtgcc tcggaaacag
     1741 aaatgacttc caatgtaccg acagataatt caggagaaac gatttcatct tcagaagata
     1801 catcaataaa tagttctacc tcttctgacc aaatcgatca tttgcaagac ttggaaaata
     1861 aaagaaaaga actcgaagaa gcattagaag ctgcaaaaaa caaattgaaa gaagaccaag
     1921 cagcaatcga tcaagattta gctgatctta aaaataaagt gggaaatgac cgtgcacaac
     1981 ttgagaaaaa gcttgccgaa gcgaaagcaa aagcggaagc tgtccaaaca gatcttgacg
     2041 ggaagatcca acaagcaaaa gaattcaatc agcaattaaa tcaggttgct caaacagctt
     2101 ctggaaagct aagtcaaact caagcggata tcacagcggc cagtcaagca atccacggaa
     2161 agatcagcga agcacaaaca gttgctaatg attttgaagc aaaaaaagct gacttagcag
     2221 ctagtttcca acgaataaaa gatattttgc attcggcagg agaaaaatca gtgacctctg
     2281 gaagcacgac agaaacagtt cagcaaacga cagagccatc caaatcaaca agtcagactt
     2341 tgccaaaaac aaatgaaaaa acagcttctg tcagttattc cttagcaggg gcgggtgtgt
     2401 tagtcgcagc agcttacgga atcatccgca aaaagaaata aaaaagagag gatgggaagg
     2461 tagagtacct cgaagttaga ctgaaaaatc taattcttga gtgccactac atgtcccatc
     2521 tctctttagt tattaaaaca gatgtcccct acttatctcg tggagacagc tgttccacta
     2581 catgtgacca tcagcatgcc attgtcgctg cctaatactt catagtccat ttttacgcca
     2641 atgatcgcat cagctcctaa tgcttttgca cgttcggcca tttcggcaag tgcttcttct
     2701 cttgcaccca ttagttcgtt ttcatagctt tttgagcggc cgccaaagat atttcgcaac
     2761 cccgcctcca agtctttaaa tgcatggatc ccagtaatga cctcaccaaa aacgatccct
     2821 ttataagcta ctacggcttt tccctctaca ctgttcgttg ttgttgtaat cattcgatca
     2881 ctcctctctt ttaattaaag tataacaaac ttcaagaagc actacacata ttttcccaaa
     2941 attagaa
//
LOCUS       DACCCR010000142         2918 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCCR010000142 DACCCR010000000
VERSION     DACCCR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.156"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(36..1355)
                     /locus_tag="IVK93_002693"
     CDS             complement(36..1355)
                     /locus_tag="IVK93_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6387927.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1352..2005)
                     /locus_tag="IVK93_002694"
     CDS             complement(1352..2005)
                     /locus_tag="IVK93_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6387928.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2293..>2918
                     /locus_tag="IVK93_002695"
     CDS             2293..>2918
                     /locus_tag="IVK93_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6387929.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPR"
BASE COUNT          956 a          475 c          322 g         1165 t
ORIGIN      
        1 tatttttcaa taactattgc aaaattacat cttaattaag aaaatatttt tattttaaag
       61 ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt cagaatatta
      121 cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa tttaacatca
      181 aatattttat ccatattcga aattttattg ttaccgtatg aattctctat atataaccaa
      241 tcattaacta tcccaatatt aattacccca tttacatcag tatattttac cgcattacta
      301 atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt ctcgtctaat
      361 atataattat tgacagttat tttcttttga tttgctaata tttcatactt ttttaatatg
      421 tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt ttctatagaa
      481 tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc aatacattca
      541 ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt ttccagtatt
      601 attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa aaagtcatat
      661 tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc aattgttctt
      721 aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc atcattagag
      781 ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac aatttttatt
      841 tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc agaaaataaa
      901 attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc atctttttgc
      961 atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag ctttatttct
     1021 ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat attatctttg
     1081 atttgtattt cattcttatt atttttttct tttatgaata cttttatttc actactcttg
     1141 gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat attactagaa
     1201 atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt tttaggaaag
     1261 ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga aaatatttgt
     1321 atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta tatcctacat
     1381 ttcttatagt agttatacag tctaattgta atttttttct aagttctttt atatatacat
     1441 ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca ataatttgca
     1501 ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct agttcttttg
     1561 catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa ttaaccgata
     1621 gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa gaatctattc
     1681 tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact aaattagtaa
     1741 atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt ggtaaattgc
     1801 ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc tgaatgtcta
     1861 agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca tctttagctt
     1921 gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt atcatattat
     1981 catcttcaac agttaatatt ttcatatata cctccactta agcctataca ctagatatta
     2041 tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt gccaatactt
     2101 tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaata aagggttcta
     2161 taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt ttcttcgttt
     2221 caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc accctaccaa
     2281 aggagtcgct ctatggataa tcatactaga aaattactcg gattaacaga taaacattta
     2341 ttttttgatg aagaatggct gattgaaaaa gaatataaag gcgttcaagc gcagtttatt
     2401 aaagggagat tggatgactc accttcccat tgtgaacact gtggatcgat tcgtattatc
     2461 cgcaacggtt cctatacgac acgtactcag atattgaaag tcaaagaaaa gttgactatt
     2521 ttagaattaa aacgtacccg ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa
     2581 acggacttag ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc gattctcata
     2641 gaactgtacg aaaatcaatc acgtaaacta atcgctaaga agtacttcgt ctcggatggg
     2701 acggtgacac ggatcttgcg tgaagcaacg aagcattacc agccacgtat gaacttcctt
     2761 ccgacggtgt tgtgtatgga tgagttcaaa tcaatgaagt ctgtttctgg ttccatgagt
     2821 tttatttgtg ttgatgggac aacccatcag ctatttacga tccttgaaga tcgcagactt
     2881 tacaaactaa ctcaatactt cctgcacttt cctagaaa
//
LOCUS       DACCCR010000143         2916 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCCR010000143 DACCCR010000000
VERSION     DACCCR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.157"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..1003)
                     /locus_tag="IVK93_002696"
     CDS             complement(<1..1003)
                     /locus_tag="IVK93_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387930.1"
                     /translation="MDDIKEVFLEVLSRYIQDNLLEYQNNGKKYEFSFDIKNYNDSLH
                     VVFQYNKAGKIGITDVHSFQIFLNEINIEASKSHLNYTTILDTNSLFFSKKLLPYLHS
                     YEWALRKLIYLLAPTYFDNWTKDSIPDEMISAVKGKQKQAIGKYDLENLLQWIDLYDF
                     EEYLFGENYLFISTGNEKNIVRYKEIIVEELLNLFRETDDLSLSDPFSLWSEVFSKYM
                     YVDLEEIRKDMELIREGRNIVSHNKEIKYSLYKDLIKNLKKYVKQLNEAFKKILIGEI
                     ADEKLFDMTNDFEAYIGNHYSNINLPNATKLLTSLSAMTVLNTDSTKKMMETIGPALS
                     "
     gene            complement(1172..2101)
                     /locus_tag="IVK93_002697"
     CDS             complement(1172..2101)
                     /locus_tag="IVK93_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387931.1"
                     /translation="MRSDSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTR
                     HIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSA
                     KTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHVRNYSQHGHLPVNIE
                     QQRVYFDLDEILTMPHFDLNEKLKSEIDEIKEDISARFEDFPRISYVYTIAKFNLITT
                     EIYLNYLKEVKPILMEMDKEKNELLLNTKFKLTNSDGKSSDVVFYDFDGENYHCFNRT
                     DNSLSMYASIKKEVKKILREEEQYYKEIKNKNQ"
     gene            complement(2349..2699)
                     /locus_tag="IVK93_002698"
     CDS             complement(2349..2699)
                     /locus_tag="IVK93_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387932.1"
                     /translation="MIFENESEEIGRVAEENKVDPLETLVINKKEFEQLFLDFFIQYD
                     MIYDFSSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFR
                     EFRNQIIRSSNIEN"
BASE COUNT          960 a          485 c          324 g         1147 t
ORIGIN      
        1 cggataatgc cggaccgatt gtctccatca tttttttagt ggaatcggta tttaatactg
       61 tcatcgctga taaagacgtc aataatttag tagcgttggg taggtttata ttactataat
      121 gattccctat atatgcttcg aaatcatttg tcatatcgaa aagtttttca tctgctattt
      181 caccaattag tattttctta aatgcttcat taagttgttt cacgtatttc tttagattct
      241 ttatcagatc cttatataaa gagtatttaa tctctttatt atgactcaca atattccttc
      301 cttctctgat caattccatg tcttttcgaa tttcttctaa atcaacatac atatacttgc
      361 taaatacttc actccataat gaaaaaggat cagatagact taaatcatca gtttctcgaa
      421 ataaatttaa caattcttct acgattatct ctttgtacct tacaatattt ttctcatttc
      481 cagttgaaat aaataggtaa ttttctccaa ataggtattc ttcaaaatcg tataaatcta
      541 tccactgtaa aagattttct aagtcatact tgccgattgc ttgtttttgt tttcctttta
      601 ctgcagaaat catttcgtct ggaatagaat ctttcgtcca gttgtcgaaa tatgttggag
      661 ccaataaata tatcaatttt cttaaagccc attcataact atgcaaatat ggtagtaatt
      721 tttttgaaaa aaataatgag tttgtatcta aaatagtagt gtaatttaaa tgtgacttgc
      781 tcgcttcaat attaatttca tttagaaaaa tttgaaatga atgtacatcg gttatcccta
      841 ttttaccagc cttattatac tgaaatacta catgtaacga atcattataa tttttaatat
      901 caaaactaaa ttcgtatttt tttccgttat tttgatattc taaaaggttg tcttgtatat
      961 atcgtgataa tacttccaaa aaaacttctt taatatcatc cattttgcct tgtttagggt
     1021 aatataaata ttcaacaact tgcttgctaa tatgtcctcg ccttcttttc tttcaacgat
     1081 ttaaaatttt ccaaataact tgataactac taagtaagcc tcaaacaaca accacaaatg
     1141 aatttttcac taatattgat gattgtgata tttactggtt tttatttttt atttctttat
     1201 aatattgttc ttcttctctt aatatctttt taacctcctt ttttatgcta gcatacatcg
     1261 ataaactatt gtctgttcga ttgaaacaat gataattctc tccatcaaag tcataaaaaa
     1321 ccacatcact tgattttcca tctgaattag ttaacttaaa tttagtattg agtagcaatt
     1381 catttttttc tttatccatt tccatcaaaa ttggtttaac ttcttttaaa tagttcaaat
     1441 atatttcagt tgtaatcaga ttaaactttg caattgtgta cacatatgag atacgtggaa
     1501 aatcctcaaa tcgagcagat atatcttctt taatttcatc tatttctgat tttaattttt
     1561 cattaagatc aaaatgaggc attgttagaa tttcatccaa atcaaagtat actctttgtt
     1621 gttcaatatt taccggtaag tgaccatgct gagaataatt tctaacatgt aataaaaatc
     1681 tgtacgagaa atgttcatca taaggcttac tcagaatttt attcttaaaa gatttaaaat
     1741 ctttttctgc taaaaagttt ttcatacaaa cttcaatgga ttcagtcaaa gttttggctg
     1801 aactaattaa gtttatcgtc aaagcattta tctgataata atactggtct tccgttaatt
     1861 tttcacctgt actttcaatt aaatcattag taaaaattgt gaagtgtttg agcaaattag
     1921 ttaaattata tctaaacaca ttaaaaagtt tatccaactc tataatatga cgtgttaatt
     1981 ctacataatt taaaaatatt cttttatcag attctgttat tgtttctaat aatgaatcaa
     2041 tctgccttat tagctcgtca tcttgtaaat tactgacgaa aaacctacta tcacttctca
     2101 taattatatt ctcctaatat gattttttca aaataatcaa tatgtaacat agaagtatac
     2161 tctattgtta cataccttat ttcttattat atcagtattt ctctgtgaca cataagggtg
     2221 taacataatt attttaaacg aaatgactac aagtagtcat tgaagcaaat ctccataaga
     2281 catttgtaaa atcttttaaa aagaagcgtc gaaagaagga acttttcttc aacgcttctt
     2341 ttattgactc aattttcaat attacttgaa cgaataatct gatttctaaa ctctcgaaaa
     2401 acttcttgtg gttgataata gtctggatct atttcaaatg tttctgagaa taattcattg
     2461 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
     2521 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
     2581 aaaaaatcaa gaaaaagttg ctcaaactct ttcttattta ttactagtgt ttcaagcgga
     2641 tcaactttat tttcttcagc cactcttcca atttcctcac tttcattttc aaaaatcaca
     2701 aaaatgatat taacttaaaa gccactaaca aaataagact tacaaagtaa agggtaatcc
     2761 aacaaaataa ccattcgctc tacgtactac gatttggtag tcctgccttt caatcggtaa
     2821 ttcataatag taatttactg tttcttcttt gccactttca atatactctg gattatccaa
     2881 tatcatagca tactcataaa gttcttcacc atcctt
//
LOCUS       DACCCR010000144         2756 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCCR010000144 DACCCR010000000
VERSION     DACCCR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.158"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(1..305)
                     /locus_tag="IVK93_002699"
                     /pseudo
     CDS             complement(1..305)
                     /locus_tag="IVK93_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(379..660)
                     /locus_tag="IVK93_002700"
                     /pseudo
     CDS             complement(379..660)
                     /locus_tag="IVK93_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1122..2504
                     /locus_tag="IVK93_002701"
                     /pseudo
     CDS             1122..2504
                     /locus_tag="IVK93_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          984 a          485 c          451 g          836 t
ORIGIN      
        1 atatctaagg ttaagccgtc cgcatcaatt gcacgataaa gataatgcca acgtccctta
       61 attttgatat aggtttcgtc cattttccat gaatagaagg attgtctatt tttcttcttc
      121 caaagataat agaggacttt gctgtactct tatacccaac gataaatcgt agtatgacaa
      181 acatttattc cacgatcata taacaattcc tgaacttcac gatagcttag attgtaacgc
      241 aggtagtaac caacagcgac aataatgacg tcttttttga attgtttgcc tttaaaatga
      301 ttcattactc tgccctctct gtcttttttc tcaattttac actaaaatag attttttgga
      361 aaactttgca acagaaccga aatgagtata gtatgaatta gtaatttggt tttcatctaa
      421 gtttagcttt gatttactaa aattgatatc tcgatacatg ttcaatacat ctaatacaaa
      481 tcgagaatct tctttcggaa atggctcaga aagatcactc caaatttcat catcataatg
      541 atactcgtaa ccttctctca atatttcgat ttttctttca taataagatc tgttttggtc
      601 ctcaaattta gccaataact caaattgatt tactagtgac aatctctgaa aactgtccat
      661 taaatgtttc ttcctatctt gttcattaac aaaaaattat aagcccttac aacgaatatt
      721 atatattaaa tttaattgta ggtgaacacg aattttataa atatttatgg tgttaatgac
      781 aaatgatatc cgactcctct tcatacgttc tggactattt gctaatagat taaaatttca
      841 gcatctattt ctgaccataa ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa
      901 ataaaggatc aaaaactttt aagtttctga ccccgtaact cacacttcaa aatcttactt
      961 aaaaaggagt ctgaaagaaa aaaagagtat tcaaaatttt tttctaacta gctacatcac
     1021 atctatgcat tgacactcaa agaaccagaa taagtcatca gaaagcaagg gggtgcggac
     1081 ataatcctgg acgaaaaaat aaacttagga ggaatgtccg tgtgttttcc cacaaagagc
     1141 gtgtaagggc aattcagtta tttttaaaat acgattgctc ttatgctgca accattcgag
     1201 aattagggta tccgtcgatt ggtgcactac ggaaatggta taaagagtat ttggaatccg
     1261 gagagctcca tcaggagcac cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag
     1321 cggtgaatca ttattttgaa tatggtcaat gttatgcacg aaccattcgc atgcttggtt
     1381 atcctaatag agagtccttg cggcagcggt gtgaagaact tgcaccagga gctagaaaac
     1441 tccgaaaaag tgcggtaaag ttaacacaag atcaaaaaga agctgtgttg aaaaaattct
     1501 ataaaccgca aactaatagg aaaaatttag ccgaatctgg aggaattagt agagttactc
     1561 tatatcaatg gaaaaacacg gttcttggaa aggattttcc attgtgtatg actttaaaaa
     1621 atcaggaaaa acaaaaagag ctacttttaa aggaagtaga agacttacaa aaacaagtac
     1681 accaattaca gctcgaaaaa gcattactcg aaggagcggc tgaactgcta aaaaaagaaa
     1741 aaggcgtcaa tcttctttgt ctatcaaatc aagaaaaaac gatattgatt gatgccttac
     1801 gaaatcagtt caccttgaaa gagcttcttc aacagctcca gtttcctaaa agcagctatt
     1861 tttatcaaaa acaagcactt gagaaaccag ataagtatta caaagaacgt caaattaatt
     1921 atcacgattt tcaatcataa cttctgtgcg tatggttacc gtagaattca ccaagcttta
     1981 aaaaatatgg gtaaaaagct ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat
     2041 ctatttgtta aattttctag acgaaaaaag tattcttcgt atgctgggga aatttctcct
     2101 gcacacccca atttattaaa tcggaatttt aaagcaaaga agccaaatga aaaatggtta
     2161 acagatatta ctgaatttag aattctcgca gggaaaatct acttatcacc acttgtcgat
     2221 tgttacgatg gtgctattgt tagttggact attggaacaa agccaacttg cttcactcta
     2281 tgtctaaaaa gagttattca ccagataatt cagcgtgcga aggtttcttt ggacgattaa
     2341 aaaacgagat gttttatcaa cgtgactgga agaatacaac aatcaatcag tttataaaca
     2401 aattggatga ttatattcat tggtataata atcaacgaat taagttatct ttaggaaggc
     2461 taagccctct tcaatatcgg aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa
     2521 tatccgcacc cccttttttt tgtaaaaaac tattgcacaa tacaagtagc ctacggctat
     2581 cgaaatttcc ataactttcg agcaagaact tatctcattc aaggcttgat cttccaagta
     2641 aaacaaaaac cagttaagca ctctgcctaa ctggttatga ctaaattttc agttgtatct
     2701 ttctctacca acactaaatg tcgaagagcc ttttttcgtt tatgaatgta agaccg
//
LOCUS       DACCCR010000145         2742 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCCR010000145 DACCCR010000000
VERSION     DACCCR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.159"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>2742)
                     /locus_tag="IVK93_002702"
     rRNA            complement(<1..>2742)
                     /locus_tag="IVK93_002702"
                     /product="23S ribosomal RNA"
BASE COUNT          574 a          830 c          567 g          771 t
ORIGIN      
        1 tagtatcagt ccgctccata catcactgta cttccactcc tgacctatct acctgatcat
       61 ctctcaggga tcttactttc ttaaagaaat gggaaatctc atcttgaggt gggcttcaca
      121 cttagatgct ttcagcgttt atcccttccc tacatagcta cccagcaatg cccttggcag
      181 aacaactggt acaccagcgg taagtccatc ccggtcctct cgtactaagg acagctcctc
      241 tcaaatttcc aacgcccgcg acggataggg accgaactgt ctcacgacgt tctgaaccca
      301 gctcgcgtgc cgctttaatg ggcgaacagc ccaacccttg ggaccgacta cagccccagg
      361 atgcgacgag ccgacatcga ggtgccaaac ctccccgtcg atgtggactc ttgggggaga
      421 taagcctgtt atccccaggg tagcttttat ccgttgagcg atggcccttc catgcggaac
      481 caccggatca ctaagcccga ctttcgtccc tgctcgactt gttggtctcg cagtcaagct
      541 cccttctgcc tttacactct tcgaatgatt tccaaccatt ctgagggaac ctttgggcgc
      601 ctccgttacc ttttaggagg cgaccgcccc agtcaaactg cccatctgac actgtctccc
      661 accacgatta gtggtgcggg ttagagtggc cataacgcag gggtagtatc ccaccagcgc
      721 ctccatcgaa actagcgttc cgatttctac ggctcctacc tatcctgtac atgcggtaca
      781 gacactcaat atcaaactac agtaaagctc catggggtct ttccgtcctg tcgcgggtaa
      841 cctgcatctt cacaggtact aaaatttcac cgagtctctc gttgagacag tgcccaaatc
      901 gttacgcctt tcgtgcgggt cggaacttac ccgacaagga atttcgctac cttaggaccg
      961 ttatagttac ggccgccgtt tactggggct tcaattcgta ccttcgcttg cgctaagcac
     1021 tcctcttaac cttccagcac cgggcaggcg tcagccccta tacttcatct tacgattttg
     1081 cagagacctg tgtttttgat aaacagtcgc ttgggcctat tcactgcggc tgatcgtatg
     1141 atcagcaccc cttctcccga agttacgggg tcattttgcc gagttcctta acgagagttc
     1201 tctcgctcac cttaggattc tctcctcgac tacctgtgtc ggtttgcggt acgggccgct
     1261 gttttctcac tagaagcttt tctcggcagt gtgacatcag gaacttcggt actattattt
     1321 ccctccccat cacagcttgt ccctaagaaa agaagcattt gactcctctt cagacttact
     1381 gcttggacag acatttccga tcgtctgcat tccttagcct cctgcgtccc tccattgctc
     1441 aaacaaaaac aacgggtaca ggaatatcaa cctgttatcc atcgcctacg cctgtcggcc
     1501 tcggcttagg tcccgactaa ccctgggcgg acgagccttc cccaggaaac cttagtcatt
     1561 cggtggacag gattctcacc tgtctttcgc tactcatacc ggcattctca cttctaagcg
     1621 ctccagcagt cctcacgatc tgccttcaac gcccttagaa cgctctccta ccaatacacc
     1681 taaaggtgta ctccacagct tcggtaatat gtttagcccc ggtacatttt cggcgcaggg
     1741 tcactcgact agtgagctat tacgcactct ttaaatggtg gctgcttcta agccaacatc
     1801 ctagttgtct gtgcaacccc acatcctttt ccacttaaca tatattttgg gaccttagct
     1861 ggtggtctgg gctgtttccc tttcgactat ggatcttatc actcacagtc tgactcccgg
     1921 atatgaatga atggcattcg gagtttatct gaattcggta acccgagatg ggcccctagt
     1981 ccaaacagtg ctctacctcc atcattctca attccgaggc tagccctaaa gctatttcgg
     2041 agagaaccag ctatctccaa gttcgtttgg aatttctccg ctacccacac ctcatccccg
     2101 cacttttcaa cgtacgtggg ttcggtcctc cagtgcgttt taccgcacct tcaacctgga
     2161 catgggtaga tcacatggtt tcgggtctac gactacatac tcaaacgccc tattcagact
     2221 cgctttcgct gcggctccgt ctcttcaact taacctcgca tgcaatcgta actcgccggt
     2281 tcattctaca aaaggcacgc catcacccat taacgggctt tgacttgttg taggcacacg
     2341 gtttcaggtt ctatttcact ccccttccgg ggtgcttttc acctttccct cacggtactg
     2401 gttcactatc ggtcactagg gagtatttag ccttgggaga tggtcctccc ggattccgac
     2461 ggaatttctc gtgttccgcc gtactcagga tcctcctagg tgtcttccac atttcgtcta
     2521 cggggttttt accctctttg actgactttt ccaagtcatt cgactatctg aaagaactac
     2581 catattggag tcctacaacc ccaacaagca agcttgctgg tttgggcttt tcccgtttcg
     2641 ctcgccgcta ctcagggaat cgaattttct ttctcttcct gcaggtactt agatgtttca
     2701 gttctctgcg tctacctcga atgtgctatg tattcacaca at
//
LOCUS       DACCCR010000146         2634 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCCR010000146 DACCCR010000000
VERSION     DACCCR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.160"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            5..1150
                     /locus_tag="IVK93_002703"
     CDS             5..1150
                     /locus_tag="IVK93_002703"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP6387933.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IVK93_002704"
     CDS             1321..1779
                     /locus_tag="IVK93_002704"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP6387934.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IVK93_002705"
     CDS             1938..2408
                     /locus_tag="IVK93_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP6387935.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IVK93_002706"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IVK93_002706"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          862 a          505 c          496 g          771 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc accccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt aaattttgtt cactcaaaac tggatttgaa gtca
//
LOCUS       DACCCR010000147         2579 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCCR010000147 DACCCR010000000
VERSION     DACCCR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.161"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            3..359
                     /locus_tag="IVK93_002707"
     CDS             3..359
                     /locus_tag="IVK93_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP6387936.1"
                     /translation="MLLTVLSFLPVMIFGLQQNTATAEKEAVLRVDGTEIKTFPLMAG
                     EESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICVRRGWATKNGETIVCLPHKLV
                     IEVRSTDGGDEDSLIY"
     gene            373..939
                     /locus_tag="IVK93_002708"
     CDS             373..939
                     /locus_tag="IVK93_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP6387937.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            962..1948
                     /locus_tag="IVK93_002709"
     CDS             962..1948
                     /locus_tag="IVK93_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6387938.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2103..>2579)
                     /gene="scm"
                     /locus_tag="IVK93_002710"
     CDS             complement(2103..>2579)
                     /gene="scm"
                     /locus_tag="IVK93_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP6387939.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          764 a          483 c          556 g          776 t
ORIGIN      
        1 ttttgctttt gactgttctt tcttttttac cagtcatgat ttttggtctt cagcaaaaca
       61 ccgcaacagc agaaaaagaa gcggtactgc gtgtggatgg cacagaaatc aaaacattcc
      121 ctttgatggc tggagaagag agcaacacat atacttacaa agatgaacac ggagattata
      181 acttgatcga gatagatggc gatcgagtac ggataaaaga agcagattgc gatgaccaga
      241 tctgcgtacg tcgaggctgg gcaacgaaaa atggcgaaac catcgtctgt ttgccacata
      301 aattagtgat cgaagtccgt tcaacagatg gaggtgacga agacagtctg atttattaga
      361 ccttcacaca ccatgagtaa attacaaaaa atgatttata tttcgctact tgttgcacaa
      421 ggagtgatca ttggtttatt ggaaaatatg atcccttatc ctttttcttt tgcgcctggt
      481 gcaaaactag gcttagccaa tctaatcacg atcgttgcca tttttaccat gaaaaaacaa
      541 gacagcttct tactactttg gctacgacta ttcctgacta ctttgctagg cggaacgatt
      601 tccacattcc tttatagtat gagtggtgct ttgcttagtt attttggaat gttattggtc
      661 aagcaattag gaccaaaacg tgtcagtatc atcggaatta gtgcggccgg cggtttcatg
      721 cataatgtcg gacagctttt gatggcttct ttcattgcac aatcatggac agtcatgttg
      781 tacttgccag tcctttcagt tttagggatt ttatcaggga ttgcaatcgg gatcgcagcg
      841 aattatcttt tacgacatgt gcagacactt caacgattcc aaaaagatta tgagcaggga
      901 accaaacaaa aatggagccc aatgtttacc aaaaaataat cgtcacacaa ggagtttttt
      961 tatgaagcta catccaatgt gggaaaacta tcctgcttta agtaaagaat tgacagaaac
     1021 actaaaacgg atggaagaaa ctgtccgcct gaaaaacaaa cctgtcgaac aagccatcaa
     1081 agaaacgatc catgccggcg gaaagctgtt gcgtcctgcc tatcagctat tgttctcaca
     1141 atttggacca gagcaagatc gagacaaagc catcgctttg gcagcttcta tcgaaatgct
     1201 ccatacggcc acactgatcc atgatgatat cgttgatgaa gcggatctgc gacgtggtca
     1261 gcctaatatc cgtagtcagt ttggtaatgt cgtttctgtc tatgcaggag actatttatt
     1321 tgtctgctgt tttaaactat tgtctgatta ctccacttcc ttgaaaagtc tccaattgaa
     1381 ttctcgcagt atggaaaaag tattgaatgg cgaattaggg cagatggatg atcgctataa
     1441 ttatgaactt tctgtaaaag aatatttgga aaatatttca ggtaagacag ctgaactgtt
     1501 ccaattaagt tgttctgttg gcgcttttga aagtggaaca agcgagcgct tcgcaaaaaa
     1561 agcaggagat atcggacttt ctatcgggat ggcctttcag attatcgatg atattttaga
     1621 ttatacaaaa gagagccagg aaatcggcaa acctgtacta gaagacatgc gtcaaggggt
     1681 ctattcttta ccgttgatct actctttgca aaaaaacaaa ccaaagctac taccttattt
     1741 agagaagaaa gcggcaatga cagaagaaga tgtcgacgcc gtacgcaaaa tcgttgaaca
     1801 tacaggaggc gtggaagaag ctaggaagtt agcggcctct tacacagaaa aggctttgaa
     1861 ggaaataaag aaattacctg ccacctctct tcgaacaaaa gagaatctct tttcccttac
     1921 ccaactgatt ttggatcgca aagactgaat agggaaaggt tcgatacgta aataaatttc
     1981 tcagaaggaa atctacgtgt tttttgacaa attgaattgt agttcttgta tttatgataa
     2041 aaagcaaaaa taaagagcaa cgtccgttag cgacgttgct cttttttatc ttgaatattt
     2101 ttttaccttt tcgatttctt ataactcata aaagtgattc ttaaaccgat tacaagtaat
     2161 ataacgcctg cgagtgttat ccaattattc gattgacttc cagttttagg aagtgctctt
     2221 tttgatttgt ttccattgtc attgcttgtt ttattagtta agttaaagtt cgtcctgtta
     2281 tccgaacttg tttttgagaa ggagacactt gcatgacgtg tatcgcttgt ggtagacgac
     2341 gtgtctgtac tatcgcttga agttgatgaa tctgagctat cacttgtggt agaggatgta
     2401 tctgtactgt cacttgaagc tgacgtgtcc gtgctatcac ttgtagtggt agatgaatct
     2461 gagctatcac ttgtggtaga ggtagtatct gtactgtcac ttgaagctga cgtgtccgtg
     2521 ctatcacttg ttgtggtaga tgtatccgta ctgtcgcttg aagctgacgt gtccgtgct
//
LOCUS       DACCCR010000148         2484 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCCR010000148 DACCCR010000000
VERSION     DACCCR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.162"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(1014..1304)
                     /locus_tag="IVK93_002711"
     CDS             complement(1014..1304)
                     /locus_tag="IVK93_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387940.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2204)
                     /locus_tag="IVK93_002712"
     CDS             complement(1512..2204)
                     /locus_tag="IVK93_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP6387941.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          734 a          458 c          349 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacg
//
LOCUS       DACCCR010000149         2473 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCCR010000149 DACCCR010000000
VERSION     DACCCR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.163"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            721..1440
                     /locus_tag="IVK93_002713"
     CDS             721..1440
                     /locus_tag="IVK93_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP6387942.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            complement(1577..1993)
                     /locus_tag="IVK93_002714"
     CDS             complement(1577..1993)
                     /locus_tag="IVK93_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387943.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1996..2280)
                     /locus_tag="IVK93_002715"
     CDS             complement(1996..2280)
                     /locus_tag="IVK93_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387944.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT          774 a          398 c          458 g          843 t
ORIGIN      
        1 taactgaaca aagcgcttaa aagcccaaca agaagggagc aaataaagtt agtgaacacg
       61 aaacacacct ccttccagtc gcaagactgt cggataggcg acaatcaatt atacaataaa
      121 aaagcaaaaa taagcctaag ttttttattg ccttggggtt gttagggctt gccctgaccg
      181 cctgtaaggc gctttagaac gaaggacata gtttttatcg aatcttgtct tagaacggct
      241 tagacgagct ctcaggacgc cacaaaggag tccctaatga attatttttg gcgaaggagc
      301 ctaaaggcga ctgctgcaag taaaatgtga gagcgtagcg atcactcctt cttacagcga
      361 ccatagggag ctagattggc agtttaatat catgcgcgaa cctgtttatt ttaaaaagtg
      421 ggtgattttt tggcgggaaa ttttgctcgc gcgcgcgcgt ttattttatt tattttttaa
      481 agatttttta gaaccctttt tagggggcag ttgagcctta ctctcccaac ggatacagaa
      541 gggtactaac aactttttat ctgagtacta acaacttttt atctgagtac taacaacttt
      601 ttatctgagt actaacaact ttttatctga gtactaatat ccttttgtat aataacaacc
      661 aacctattta cgagttggtt gttaaaggtt attatttatt tgtaaggggg gaagaaagga
      721 atggatgaaa aaacggaatt aacagtcaaa tatcaaaatg aattgaattt agtaccgctg
      781 aaaaatttca atgctaagca gatggattta ttttttgcat tatgcgctcg tatgaaagat
      841 cgtggtttga aaaaagttag attttctttc gaggaattaa aagagttaag tgattataag
      901 atgactgcta caaaagcctt tgtcggtgat ttagaaaagc tctataaaga tatgcttaat
      961 cttacttacc gtactgaaga tgaggattct attcgctact ttgttttgtt tactggtttt
     1021 gaaatcaata aaacaaaaaa gtttgttgaa gtcagtgtta atccggacct agaatatatt
     1081 attaatggtt taacagctga atttagtaga tttgaattat ctgctttcac gtcgttgcgt
     1141 tctacgtatg caaaaactct attccgactg cttatgcagt accgttctac tggttattat
     1201 gtagttcgta ttgaagagtt taggagctta atggatatcc cagaatacta tcaaatgggt
     1261 aacattgatc aaaaagtatt aaatcctgca ttacgagaac taaaaaaata cttcaatgat
     1321 cttaaagtca caaaaataaa agcaaaaaag ggcaataaaa tcgccaaatt agaatttact
     1381 ttttcaagcc taacgtcaga tttgccaaag atttctctgc gtaactggtt agaagaataa
     1441 ataaaaaaga actcgccccc cttacattaa gcaagtagac aagtccctta caaccgacta
     1501 agattgctag tacattctag catattgtat ggggcttcta aataaaaaac agataggaat
     1561 aactcctatc tgttttttat cgttttagaa aatttgttgg gtcgaaattt ctagaagtga
     1621 ctgtttgact ggtttctttt tgatgattct ttggtcgatt tcgatttgat ggatttagtt
     1681 tagcttcgat tttttctttc acttcttgat atactttaga atttgtgcca ataagaacgc
     1741 ctgtatcttt tagatataaa tcagcaaatt ctgcgatgat ttcagtcatg atggaaattt
     1801 cttttcccat agcatttagt tttgcctgat tgctggtagt ggtcgtgttg gtatcgggtt
     1861 cataactcat acataaaaag cgaatggctt ccgagtagtt attgatatct ggattttttt
     1921 cgatgatgga atctaaaacg tcaacatgac tagggtacaa agtaacatgt ttttggacag
     1981 aagtttttct catggttata tcattcctcc gataagcatt tcaatgattt gattatttgt
     2041 gtagacatac tcgcttaata tttgagtatt ttctgtttgg atagcaataa atttatcgag
     2101 caataaatcg aattggtctt ttttgtaagt cgtaagttga cggtcaaaat cactttcgat
     2161 taaaactttt agatatttat ttcgactcca tttttctcct gttttattcg tgagttcttg
     2221 gcaacgtttg tctattgttg caacaaactt tggatcaata ttccgaacta aaatgtccat
     2281 aaaaaatctc cttttgcaat tatgtataat ttatgtatac catacaaata ctatatttgt
     2341 atagctgttt tttgccaaaa tgctatacat aagctaccca cgatggcgaa aaagtgatat
     2401 caaatttttt tctgagaaac gtgataggca cagcctatct acccactttt tttagaaagt
     2461 gggtacacgt ttc
//
LOCUS       DACCCR010000150         2399 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCCR010000150 DACCCR010000000
VERSION     DACCCR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.164"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            207..2069
                     /locus_tag="IVK93_002716"
     CDS             207..2069
                     /locus_tag="IVK93_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6387945.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2399
                     /locus_tag="IVK93_002717"
     CDS             2069..>2399
                     /locus_tag="IVK93_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6387946.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DACCCR010000151         2382 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCCR010000151 DACCCR010000000
VERSION     DACCCR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.165"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            807..1007
                     /locus_tag="IVK93_002718"
                     /pseudo
     CDS             807..1007
                     /locus_tag="IVK93_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1673
                     /locus_tag="IVK93_002719"
     CDS             1494..1673
                     /locus_tag="IVK93_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387947.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1719..2324)
                     /locus_tag="IVK93_002720"
     CDS             complement(1719..2324)
                     /locus_tag="IVK93_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6387948.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          787 a          393 c          424 g          778 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atttaaaaag aaaagtgaca
      361 gataggaata aaaacttatt aaatgtagtt atgatctgtt atatttcatt tctaatacta
      421 ggtcttccac aagcagtgtt tcctgaaatg ttttctggag actggggtat tgtcagctat
      481 tctatattaa cattgattgc tatggtttta ggtacttcat tttataatgc ttttgtaagt
      541 aagcatgttg aggaatatga agtattaatg tacttaaaag agaaaaaaac aagtaattaa
      601 tatgagaaca ccacgcatag tttttatttt gagatattct agaatgaaac acattaagaa
      661 cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc taagtttttt catatttgtt
      721 tgttatgcta tacatagatc tgcataatta gacttgcgct gcccgttatt gaagtttcag
      781 gcagcgctct ttttggggga tcgataatgg aagaagaaaa aatatttgag aaacgctggc
      841 agctagcatc aagtgaacaa agagcaagat acaataattt gatcacctcc taccccactg
      901 ttgactagac atataaggaa aagaaatact tactttggct gtgccagtta gatatagata
      961 cctttgaaac atttgaagtg atcctagata aaattaaaca gaattgatca gaaacaagcg
     1021 aagccttctc tcggttcgct tgttttgcta tttattagtt ggctcagggc aactctaagc
     1081 tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta ccactttaga atacatggat
     1141 gtacgtattg ccattgactc ctattagtta ctgaaaaaaa atttaaacaa gttgagtatt
     1201 gcagataaat agctccagtg aggtgaataa ctgctccact caaggcaata gatagctcca
     1261 tccagggcaa caaactgctc cactttcata tttaatagat gaataacctt ctgtataata
     1321 gaaaggcata cctttcggta tgacaactta tacagaatgt tattttattg gttcactatc
     1381 gaaaaatttt ggagctttat ttttccggta tttctcaacg aacgattagt ttaactgtgg
     1441 gaagctccag acatacgatt cgagaagtta cttctcgagc agacaggaag ggggtgactg
     1501 aactaaccgc tgatatgagc gatacttggc tgcaggaata tctttttccg gaaaaacaaa
     1561 caacagcacg aggttacctt cctgtagact gggaatatgt tcataaagaa ctaatgaaaa
     1621 agaatatgac attgaaactc cttcatagag aatatggggt acatggaaca tagaaacgaa
     1681 aatgcacggt agaaaagcga atgaatatag tataaattct acaaatcatt cgctttcttt
     1741 attttatata tggttggtcg agagagacca ttttctttgg caatttctgc aactggaact
     1801 ccttgattta acatgtccac cacagtttta aagactaatc gtttttgcgg atctgttgag
     1861 ttctcagaat attcaattgg tcgcccctta tatcgaccag cttttttggc caataatact
     1921 ccttgccgct gacgttcacg gattttctca cgttcgtttt gtgcaatata acttagcaat
     1981 gataaaaaca tatcgaacat agcagtatct aggagttcat ttccagtatt aaaatctaaa
     2041 aactgtgcat ctagtatttt taatgctact tttttctgtt tcataaaggt aactgtattt
     2101 tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac acaatcacct
     2161 tctcggatat aatttagtaa tttttgaaac tcaggtcgct ggatatcttt accactggct
     2221 ttttcttgga ataatttatc aatgttaagg tcttccatca ttgccaattg tcgatctaaa
     2281 ttttgatctt gtgaagacac tctaatatat ccaatttttg ccataaaaac tccttttaaa
     2341 aaatgatata atttacatgt aaaatttact tgtagtttta ct
//
LOCUS       DACCCR010000152         2352 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCCR010000152 DACCCR010000000
VERSION     DACCCR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.166"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            404..622
                     /locus_tag="IVK93_002721"
                     /pseudo
     CDS             404..622
                     /locus_tag="IVK93_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1117..1716
                     /locus_tag="IVK93_002722"
     CDS             1117..1716
                     /locus_tag="IVK93_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6387949.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1780..>2352
                     /locus_tag="IVK93_002723"
     CDS             1780..>2352
                     /locus_tag="IVK93_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387950.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT          831 a          368 c          427 g          726 t
ORIGIN      
        1 actcttttaa caaggtatac aaaagatatt tatccattcc attataaata aatatttcct
       61 tatcttccga tttgattctg caagctagtt ttggcttggt gtcaatttgc gatttttgag
      121 ccgcacttga aaattgaact ggtacaatat cttttttatt cattcgtttt tctccttcta
      181 tatttgatag tttaattata gaagaaagat aatcggcgta cgagatacta tcctatgaca
      241 cgcttacatt taatttaata ttttgcctca aattagccac actttttata gaactaagag
      301 gaaaaatgaa gttttcttcc tattaatata gttgtttgaa atcttaaata tagcgtttta
      361 cgatgtaatg ggaagtatgg tgaataaatc aactatgttc gtacgaagca tttactcaac
      421 taatttaatc gaatctttca ataaacaaat caagaaatac agccaccgca aggaacagtt
      481 ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt acaaccaaaa
      541 attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg aacaaatgct
      601 gagtcaaccg atggagaatt agaaacaatc tacagtaggg aagaaccatt gacacaaaat
      661 tattgacgct cccaactaaa agtgtcaaaa taatgatgaa atcatttagt tcttataaat
      721 tagaaaagag aaaataaata ttccatatct actttattgg atccgtaaca tgactaaatt
      781 ctgaatactt tttaaagctt atcatttggt aaaactgaat ataagttaat tatatagact
      841 gaaaattttg aattttcaga atagaataac ttaaaagtac tgacaaaatt ttttaaaggg
      901 gcaatattaa tgttctgaga taatcaaatc ctaccgaaaa tatcaaaaat ccaatgtgac
      961 acaatcagat tgtgtctcat tcgagggaat cacacaatcc cacgtggctc taggcagaat
     1021 tgaatttttg cttggggctt tttccttata ttgattttga atgagactta ttatatgtta
     1081 taatgcaatt aagtatcatt ggagagggcg aagaaaatga agacggtaga acctattcga
     1141 gacaaaaaaa agatcgatgc gatgaaggca atcttggctt cgggaaaata tggccaacga
     1201 aatttggttc ttttttcgat tgggatcaac accgcctatc gcatttctga tttgcgtcaa
     1261 ttgaagctgg ccgacgtgct ggaaatttca cgcggtcgcg tggtggtcaa agatcgatta
     1321 gcgatgaagg aacaaaaaac agctaaacat aattcggtct atatttccaa caaattgcga
     1381 aaaacgattt tggagtacgt tcaacaggaa tttcctgatc aactccttca gcaagatttt
     1441 gacggttatt tgttccctag tcgaaaaggg acgaatgaac cgctcaaccg ccaaagtctg
     1501 tggcgaatca ttcatgatgc tggcacggca atcggattga aagacattgg cccacattcc
     1561 atgcgaaaaa cgttcggtta ctttctctat aaacagggga ccaaaacaga aatcattcag
     1621 tccttgttga accattcttc tcagcgcgaa acattgcggt atattggtat tacacaagaa
     1681 gacaaagata cagctgtaaa aagcttagat ttataatata aaagttaagt ttaaacatgg
     1741 atagttttaa attagtagat tgagtgttgg gaggaaagaa tgagtacatt atatattata
     1801 ggaaacggtt ttgatttaag tcatggttta aaaactagat acactgattt ttcaaaatgg
     1861 gttgaaaaaa gtcatagtga aatattcgat aaaatagaca caatgataaa tgaaaataat
     1921 gaggtgagaa gtacagctga tgataagata ggttggttta attttgaaag tagtttgcat
     1981 gagttaattc aaagtccagt ctttgaagat tatgtagaag agtctaagga ttatctcata
     2041 ggttatggac atgaagattg gtcagattct gcccatcatg attttcaaaa tatggtttat
     2101 gattatgttg tttctctaga agaattacaa caacttttca aggaatggat acgcaaagaa
     2161 atacagccga caatagaaca taaaattcca aaataccaat tttcgtccga tgtactattc
     2221 tttacattca attatacaga tgttttggaa attctataca agcaaccgaa agaaaacata
     2281 tatcatattc atggcagtta tgacgatata ataatcggcc actccgagaa ttttgacaac
     2341 tttataggag at
//
LOCUS       DACCCR010000153         2328 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCCR010000153 DACCCR010000000
VERSION     DACCCR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.167"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            656..2083
                     /locus_tag="IVK93_002724"
     CDS             656..2083
                     /locus_tag="IVK93_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HAP6387951.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
BASE COUNT          772 a          405 c          412 g          739 t
ORIGIN      
        1 aaaagttctt cgtgttctct tagacataaa aattcctcct ggtatgtttt cttctagtct
       61 acacacctta atttttctgt ctagttaatt gtagcctatc cactgctatg tatatgaaat
      121 atgcagaagt ccctagtttc ttatctcctt cacctgcgaa acatgtatct aacggaaatg
      181 cttgagctaa aaacgttgta tctggtaatg atattttagg gagcttttac tcagaataca
      241 aagcttgggt gcgtggaaca agcaagacaa aaacaataaa aggaacattt gcttttacaa
      301 gtggagacaa gtactatgca ttacacaatg ttaaatattc atattccttt gtgagacagt
      361 caaacggatc ggttaaaaca actggtgtag taacagacgt ttatgatttt gctccttcta
      421 attacgaaag tattaaagta ggttttgcta acaattatgc ttacgcaatg caatcattag
      481 gactaatcaa accctttaac atcgaaatca aacactcatt ttaaattgaa taggcaaaaa
      541 aaaacagacc atttcattgg tctgtcagac tgtagacaaa ctcctaaatt gggagtgttg
      601 tttgcagttt ttttgtttta tactcattgt ataaactatt ctgggagtga ttctaatgat
      661 gagaaaacaa tcgattgaag gtagaaatca atttgctatg ttaacaattg atgatcttgt
      721 acctaaagat cacttagttc gaaagattga tgctgcgatt cagtttgatt tcatttatcc
      781 cattgtagaa tctacgtatt cgacccatgg tcgtcccagt attgatccag tggtcttaat
      841 taaattagta tttatccaat atttatttgg tatccgttcg atgagacaga ctattaaaga
      901 cgttgaaaca aatgttgctt atcgttggtt tttaggttat tcattcgagg atcctatccc
      961 gcatttctct acttttggta aaaactatgt tcgaagattc agagaaacaa ctttatttga
     1021 agacattttc tctcatattc tagaacaagc tgtaaaagct ggcttcgtta cggaagacaa
     1081 cctttatatt gattctactc atattaaggc aaatgccaat aaacataagt tcacaaaaga
     1141 aatgacctac ggtcaagcta aagcttatca agatgaatta gaagatgaaa taaataaaga
     1201 acgtattgct gtaggaaaac gtccttttac atgggacaca gaaagtgagt tgagtcttca
     1261 aaaaataagt cattcagatc cagaaagtgg ttactatgtt aaaggtgaac gagaaaaaca
     1321 atttgcttat tccgcgcata cttcctgtga agataatggt tttatcttat ctacattaat
     1381 cacaccagga aatgtacatg acagccaagt cgcaatcgat ctggtcaaac agtcgaaaag
     1441 agcttttcca aatataaagc gtgttgtcgc tgatgcagga tataagacgc ctaaatttgt
     1501 tcattttctg tcacacttga aactctaccc gattcttcca tatactgtac ctcatggagt
     1561 aaaaggaatg tttcgtaaaa aagactttgt ttatgatgcc tattttgact gctatttatg
     1621 tcccaataat caaccgttgt tattttcaac tatcactcgt gatggttatc gaacctataa
     1681 atcaaatcca aaattctgta ggagttgtcc attacttaaa aactgtactt tgagtacaaa
     1741 acatcaaaaa atagtaactc gacatgtttg ggcggatctt atggatgaac ttgaacacca
     1801 acgccatacg gcgttgaata gagaaattta taaaaagaga aaacaaacca ttgaacgaat
     1861 atttgcagat gcaaaagaaa agcatggtat gcgttggacg aaatatcgag gtctcgaaaa
     1921 agtcgccacg cacacgatgc ttacgtttgc tgccatgaat ttaaaaaaac tagccacatg
     1981 gctatggaaa gcaaaaaagc ctatggcttt ttatttaaaa tttctacaaa gactaacaaa
     2041 aaggagttgg caattaagcc actccttttt gtctacagtc tgacagacca tttcattggt
     2101 ctgttttttt gatctcatga ttaatttaac agaattaggc agaagtcgcc catcctctat
     2161 aggtgggtga tgaatgctgt tcggtatgag ggttaccgtt ggtaactcga agaccgacaa
     2221 ctaaggcgta ttgccttttt attttgtccc atgttacaat cagtctatac aagaaatgag
     2281 ttgatataac atggaattag atacaaataa tcattcagta tttcttct
//
LOCUS       DACCCR010000154         2265 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCCR010000154 DACCCR010000000
VERSION     DACCCR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.168"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            107..754
                     /locus_tag="IVK93_002725"
     CDS             107..754
                     /locus_tag="IVK93_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387952.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            954..1751
                     /locus_tag="IVK93_002726"
     CDS             954..1751
                     /locus_tag="IVK93_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387953.1"
                     /translation="MVVSKLPRNEDYLTNYDGYVVTTEHFDLANPVTKVDDIPDYEMY
                     SQTIDSLNKRFGNRVLKGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPIL
                     DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFD
                     DLFALLKAKGVTELAIYQKGKRIMVPLPVQKNVLSIKSEDVFLDFFF"
     gene            complement(1734..2231)
                     /locus_tag="IVK93_002727"
     CDS             complement(1734..2231)
                     /locus_tag="IVK93_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6387954.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          724 a          373 c          415 g          753 t
ORIGIN      
        1 ttttttgtgt gatttatttt cttttctaat tcaacctgtg gtatgatgag agcataagta
       61 tcatgtgaaa tgatatgaag tatgtggaag atggacgggg aagaatatgg aagcaaaaat
      121 cactttagaa ccttttgaac gtatcttaag cggttaccgc aaagtagaag agttagctgt
      181 gaacgtaact gattgttcaa aattagcgca aaaatacgca agattcggcg ttgaaggcta
      241 tcgattagga aactatgtcg gtacagggta tctgaaccgt tatttagagt gtatggtgga
      301 ccgcgcacca atgctgattt atagacaaaa ttatctgatt ccgctgcttt ttcgtcgttc
      361 tgattcagcc tttcggttat ttgaagaaga gtatcggatg gaggctttct ttctactatt
      421 agagtggagc ttgaaacatc gtcctgaaaa aattcttatc gaaagaaatg agaagataga
      481 cacgaaaaaa aataaagtga tcgatagtgc atacctagca tttcgtgtat cagagatttt
      541 ggattgtggc ggctatccaa tcagcaattt ccaatcaatt gaccaattca ttgaatggaa
      601 tcgaatttac cgattgatcg acaatggcgg gattggccgt catagcaagg tatttgaccc
      661 agaatatcca gaaaacatgg aagaactgaa aatgattatt tcgcttgtca aattaaaata
      721 tcctgaaaca gatttggatt tatatataga ataaatgacc ttaaatgatt gaattacttt
      781 ttctgcaatt attattgtaa ataaaaagac aactgctaac attaatgata gcagttgtct
      841 ttttatttac agtacttttt tctcataaga tgaaatatcc acccgtggtg ctagttaatt
      901 ttttcctaga ggaaaatgtc taaacatgtt ggtaaatcaa caaaaaaacg catatagtag
      961 tctcaaaact cccaagaaat gaggactatt taacaaacta cgatggttac gtcgtgacaa
     1021 ctgaacattt tgacttagcc aatcctgtta ctaaagtcga tgatatcccc gattatgaaa
     1081 tgtacagtca aacaatcgat agcttaaata agcgttttgg caatcgtgtt ttaaaaggca
     1141 tcgaaatcgg ttacatcgct tctgaaaagg atcggatcat cgactattta gccgataaag
     1201 attacgattt aaaattactc agtgtgcatc ataatggtca atttgattat ttagatgacg
     1261 aagtcaaaga tatggatcct gctattgtga ttcctcagta ttttgcacaa ctttcagaag
     1321 cactagttgt tatagaagca gatgtctttg ctcattttga ctatggtttt cgggtatttg
     1381 gtctatccgt tgctgaattt aaacaatatg aagcgcagtt tttaccgatt ctagatcaag
     1441 taattaaaaa taaattggca ttcgaattaa atgctaagag cgcttacttg tatgacaatt
     1501 tagcgttata tgaatatgtc atcgatttat atctttcacg tggtggtacc ttgtttagtg
     1561 ttggatcaga tgggcattac cttgaacatt tccgtttcca tttcgatgat ctttttgctc
     1621 tgttaaaagc aaaaggtgtg acggaattag cgatttatca aaaaggaaag cgaattatgg
     1681 ttccattacc agtccaaaaa aacgtcctca gcatcaaaag tgaggacgtt tttttagatt
     1741 tctttttcta aaatcggaca aatacgatct tgttttccta aaggaaaatg tttgatcccg
     1801 atttttttaa atccatgacg ttcataaaaa cgaatacctg catgattttt ttcataaaca
     1861 gtcaaacgta aataacgttt ttgtttcgtt ttagcataac gattcgcttc atcgagtaac
     1921 cgttgcccta accctttgtt ttgataatct ttgagcagat agagccgttg aatctctata
     1981 gcatcttttt catcattgac tttcatataa ccaatgagta tttgatgatc cttaatacca
     2041 ataaatttac tggttgactg tgccaattct actgccaata tttcagttgt caattgtgtc
     2101 tgtaaatatc cttgcatatc aagtggatct gcgccatcga taaacgattc ataaaaactg
     2161 acaacactta caacttgtaa agcatctaga tcatcataat ctaaatacgc tagtgtgatt
     2221 tgattgttca tctaactcct ccttttgaca tagtatacta taaat
//
LOCUS       DACCCR010000155         2194 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCCR010000155 DACCCR010000000
VERSION     DACCCR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.169"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            278..802
                     /gene="infC"
                     /locus_tag="IVK93_002728"
     CDS             278..802
                     /gene="infC"
                     /locus_tag="IVK93_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP6387955.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            874..1074
                     /gene="rpmI"
                     /locus_tag="IVK93_002729"
     CDS             874..1074
                     /gene="rpmI"
                     /locus_tag="IVK93_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP6387956.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1127..1486
                     /gene="rplT"
                     /locus_tag="IVK93_002730"
     CDS             1127..1486
                     /gene="rplT"
                     /locus_tag="IVK93_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP6387957.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1838..>2194
                     /locus_tag="IVK93_002731"
     CDS             1838..>2194
                     /locus_tag="IVK93_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP6387958.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
BASE COUNT          735 a          384 c          479 g          596 t
ORIGIN      
        1 aatcactgct agaaagcctg ctaatctaat cattttcaga aaaggaagac aaactgtatg
       61 aaaaacattg acaaaaagat tagtttttgc tatactataa tggttgagaa ataaaagcag
      121 aagcacccgc ttctcgcctt agttgataat gtcactgggc tagatagttt tcactggttc
      181 tattaggtga agtacgcgcg ggttctgtaa gagaactcgt ttttttattg ccgtttattc
      241 ggtaagcttt tctttctctc ataatatttg gaggtgaatg accatagcaa aggatatgat
      301 ggttaacgac ggcattcgtg cacgagagtt acgattgatc ggttcagatg gtgagcaatt
      361 aggagttaag acaaaagcag aagcattgaa tattgcagaa caagcaaact tggatcttgt
      421 gttagttgcc cctggtgcga aaccaccagt tgcgcgaatc atggactacg gtaaataccg
      481 tttcgaacaa caaaagaaag accgcgaagc gcgtaaaaaa caaaaagtga tcaatgtaaa
      541 agaagttcgc ttaagtccta cgatcgactt gaatgacttc aatacaaaac ttcgtaatgc
      601 acgtaagttc ttggaaaagg gcgataaagt gaaagcttct atccgtttca aaggccgtgc
      661 cattacccat aaagaaattg gtcaaaaagt cttgaatcgc ttagctgaag aaactgcgga
      721 tattgcaaca gtggaacaaa aagcgaaaat ggacggacgc agcatgttct tgacgctggc
      781 accgaaaaac gacagtaagt aatttgaatg actaactggc tgttggacag ccgatagtcg
      841 gataacgaaa gattttgagg aggaaatata gtcatgccaa aacaaaaaac acaccgcgga
      901 tcagcaaaac gtttcaaacg tactggtaaa ggcggattga aacgcttccg cgcgtttaca
      961 agtcaccgtt tccacggcaa aacaaaaaaa caacgccgtc aattgcgtaa agcaagcatg
     1021 gtttctagtg gcgacttcaa acgtattcgc caacaactag caaaaatgaa ataagaagta
     1081 caaataacag aatctgaaga tttattttta ttaggaggaa ttatacatgg cacgtgttaa
     1141 aggtggaaca gtaactcgta aacgtcgtaa aagagtgcta aaattagcaa aaggttacta
     1201 cggttcaaaa cacactttat ttaaaacagc aaaagaacaa gtaatgaatt catacaacta
     1261 cgcatatcgc gatcgtcgtc aaaagaaacg cgacttccgt aaattgtgga ttgctcgtat
     1321 caacgcagca gctcgtatga atggcttgag ctactcaaaa ttaatgcacg gtttgaaagt
     1381 tgctgaaatc gacatcaacc gtaaaatgtt agctgacttg gctgttaacg atgcagcagc
     1441 atttactgca ttggctgacc aagcaaaaga agctttggct aaataagcta aacaattata
     1501 aagaggaaag aagataacga aaagttatgt tctttcctct ttttttattt acaatcctcc
     1561 atcaaatgaa acaggacaat aacgtataag tctaaaataa cataattaag gttttttaat
     1621 tgaaaactga acatttatgg tcatccttta atgaaagcgc taactacata agttagaatg
     1681 agtttgactg atatattagt ttgcttatca gaaaaacaaa atggacggag gaataacatg
     1741 agtgatatta ttattggtat tttgttagtt atctcattct ttttcatggt ctggtactgt
     1801 gtaaaaggct acaacttgat ggtcggcttt tgtcatcatg gctactgtat ggatgggatt
     1861 agcgctagtc ggaaacacgt tttcaccaaa ctcggcgatg gaagggcaag gagtaattga
     1921 tgtgttgacg catatctata cgacggggcc agctgagtac gccaaatcga tcttggtaaa
     1981 tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat agcggaatcg cagctaccct
     2041 gattcgaaag gtagttgaac ttggagggga caagccaaga atcacgatga gcttactatg
     2101 tattgtaacc gctgttatat tcatgtcgat gacaggaatc ggaccagtga tttctattgc
     2161 agttattgtg ttgccgatct tgatgtcatt aggt
//
LOCUS       DACCCR010000156         1941 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCCR010000156 DACCCR010000000
VERSION     DACCCR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.171"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            174..476
                     /locus_tag="IVK93_002732"
     CDS             174..476
                     /locus_tag="IVK93_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387959.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(992..1065)
                     /locus_tag="IVK93_002733"
     tRNA            complement(992..1065)
                     /locus_tag="IVK93_002733"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Ile,seq:cat)
     gene            complement(1620..1934)
                     /locus_tag="IVK93_002734"
     CDS             complement(1620..1934)
                     /locus_tag="IVK93_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387960.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          706 a          289 c          301 g          645 t
ORIGIN      
        1 agggtttgag cattttttat tttcgcagat tttcgttaaa tgaatgaaaa tggcattcaa
       61 aatagcattc aaaaagaaca ctttttaata tatatgttcc atccatatta tcaatgatat
      121 tcctacttta caaaaagaac attagttcgt atactctttt tgaggtgaac attatgttga
      181 tggaaggaaa aacacaatta tggtttaaat ttgatccttc gaatagattt gtcaaagatt
      241 tttataaggt gtgggattca gaagttttct ttttagcaat cgaagatagc ttattaatca
      301 atctctatta ctctaataag aactacttta aaattccggc aacaaaaacc agaatgaaga
      361 aagatgtgta ttttttgttt gatatcgtga ctgatgtgcc agacgcacgg agcgatcatc
      421 ggcgttatga ctatttgaag tatactttcg ttgatccaga aaggtataaa gattaaagta
      481 ggctacctaa aaaaggtagc ccggaacgga ttttatcacc atacttataa aagggatatt
      541 ttttaagtta gtattaagat tgtgtaatat gatgatatct atattttata gtatcagtgc
      601 tataaaatca aaaataagtc actaattaac taccactcca attataagtc tttttcccat
      661 tattttttac agttatatgg tatgcttttt aatggcttca aatataaaag agtttaaagc
      721 gtaacacact tatggggaag tggtttttgg ggaacgcttt aaactcttct ttattattat
      781 ctcacaatat aacccaaatg tctttccatt taaaaatcaa agtaaaactt ttcaaatata
      841 cagaagtata actatgtgaa acatcctttc attaatccat aaaaggatac ataaaaaagc
      901 cactcatttg agtggcaatg aagaaaagct ttagcttgta taatactctt caaaaaattc
      961 taacacagaa cgattcaatt ggctacatta atgtaccctg taggactcga acctacgacc
     1021 ggacggttat gagccgtctg ctctgaccaa ctgagctaag ggtagtggtt gttgccacat
     1081 aaagccataa acaatcaacc agtagaatgc gtggcaacaa acctgttatc gcatatcttg
     1141 gagtgtgact atttatgagt gatagtgaag atatgcgtct ccatatatgt atggttgaga
     1201 cagataaaca tgtatgaaaa acgttgttaa atcaacgttt ttttattttg gtcaaggaga
     1261 gaaaagtaga gaaatacaaa attttttgca ttgattttgc attcaaaaaa acattcaaac
     1321 atttagaaat ccccaatacg tattcaggaa aatctatttt aaggtttaca aaaagaactt
     1381 ttgttcgtac actctttttg aggtgaactt ttatgctgat ggaaggaaaa acacaattat
     1441 ggtttaagca tgttccttca aataagtttt ttaattataa actctagaga aattaattca
     1501 tcctaacata aaataattgt tcactcaaaa gtttttctag aacaattaaa acagcaggct
     1561 tcacattcgc tatacatttg taagaagtaa taattcctgt actaaaaggc ataattgctt
     1621 taattatttt ttgacttctc ttcaaacaca agaagtagtt gtgtaataat tgcagaaata
     1681 aggattccaa taatagatat aaaatttgac atggaaacgt ctataacatt aaaagaataa
     1741 agtgttgaag aaagtaaact gattgaaata acccctccgt aaaaaatagt ctgtgaatat
     1801 acccactttt tttcagaaga cgtacttcgt ttggttctaa aacctatccc tgcatttcta
     1861 ctcttatcaa aaaaccaaat atttagaagt aataatattg ctattattaa gataaaatta
     1921 ctgtaaaaat ccaaaagcta t
//
LOCUS       DACCCR010000157         1918 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCCR010000157 DACCCR010000000
VERSION     DACCCR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.172"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            153..1859
                     /locus_tag="IVK93_002735"
     CDS             153..1859
                     /locus_tag="IVK93_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP6387961.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          685 a          382 c          345 g          506 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataa
//
LOCUS       DACCCR010000158         1916 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCCR010000158 DACCCR010000000
VERSION     DACCCR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.173"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            6..398
                     /locus_tag="IVK93_002736"
     CDS             6..398
                     /locus_tag="IVK93_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6387962.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..1838
                     /locus_tag="IVK93_002737"
                     /note="aph(2'')-Ia"
     CDS             399..1838
                     /locus_tag="IVK93_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP6387963.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          806 a          138 c          326 g          646 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat ggtggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa
     1081 aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa
     1141 atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg
     1201 aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt
     1261 ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac
     1321 aaatgcacgg tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg
     1381 tattagaaga gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa
     1441 aagattatat agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa
     1501 agtgtttatg ccataatgat tttagttgta atcatctatt gttagatggc aataatagat
     1561 taactggaat aattgatttt ggagattctg gaattataga tgaatattgt gattttatat
     1621 acttacttga agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa
     1681 tgtatggaaa tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt
     1741 atcctattga aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg
     1801 gtagaaaaga aatttataaa aggacttata aagattgatt atataatata tgaaaagcta
     1861 ttataaaaga cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagt
//
LOCUS       DACCCR010000159         1891 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCCR010000159 DACCCR010000000
VERSION     DACCCR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.174"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            192..290
                     /locus_tag="IVK93_002738"
                     /pseudo
     CDS             192..290
                     /locus_tag="IVK93_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(513..743)
                     /locus_tag="IVK93_002739"
     CDS             complement(513..743)
                     /locus_tag="IVK93_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP6387964.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(973..1791)
                     /locus_tag="IVK93_002740"
     CDS             complement(973..1791)
                     /locus_tag="IVK93_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6387965.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
BASE COUNT          667 a          308 c          274 g          642 t
ORIGIN      
        1 accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt tactgataga
       61 cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat tttggcaatt
      121 tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat tcaaacatta
      181 gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga acacctttgg
      241 aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga caattaagag
      301 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
      361 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      421 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      481 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      541 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      601 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      661 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      721 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      781 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      841 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      901 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      961 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
     1021 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
     1081 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
     1141 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
     1201 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
     1261 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1321 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1381 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1441 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1501 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1561 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1621 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1681 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1741 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1801 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1861 ccatttttat ctttactttt atgaaagcgg a
//
LOCUS       DACCCR010000160         1873 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCCR010000160 DACCCR010000000
VERSION     DACCCR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.175"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            49..1437
                     /locus_tag="IVK93_002741"
     CDS             49..1437
                     /locus_tag="IVK93_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAP6387966.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
BASE COUNT          758 a          230 c          302 g          583 t
ORIGIN      
        1 tcaaaaaaaa accaaccgaa agatataaaa gatattgtag atacacgaat gtataaagaa
       61 cgtgatgact taaactatac gcttattatc aataaagatg gaaactattc atggcgtcct
      121 ctcgttctac tacatcctat tctatatgta gatttggtta attgtctcac taaagaagac
      181 cattgggagg agttaaaaaa gaggtttagt gaatttaaaa aagataaaaa tattaactgt
      241 tattctatac ctgttgaagc ttccaaaggt tcaaaaagga cagatcttgg tgaaacaata
      301 ctaaactggt gggaatattt tgaacaaaaa agcttagctc agaatattaa ataccaatat
      361 tgcatgttta ctgacatcgc tgattgttat ccatcaattt atactcactc tataacatgg
      421 gccatgtatg gtaaagaaaa agttaaaaat gatgaaaata agtgttcggg ctcacttggc
      481 gataaaatag ataaaaaaat atctggaatg caacacaatc aaacaaatgg tatccctcag
      541 ggttccgttc ttatggattt tgtagcagag atcgttctag ggtatgttga tttgagatta
      601 tcgaaagaat taaaaaaatt agaaaattta aaagattaca aagtactcag atatagagat
      661 gattatagaa tattttcaaa taatcgtaat gatttggaac aaattatgaa agtgttatca
      721 gaagtcttat atgatttaaa ttttaagtta aattctagta aaacaaaatt atcagatgat
      781 attatcatag attctatcaa aaaagataag ctctattggg aagaaattaa ggcaagtata
      841 agaatgaatg ccacaaactt agatggtgat agagaaattc gttatagtat aaatttacaa
      901 aaacatttat tggaaataaa aaagttagca gataagcatc ctaattcagg ccaattgaaa
      961 aaagcacttt ctgagtttaa taaagaacgc attattaaaa tcacttcaaa gccgaacgat
     1021 cttttaccac ttattggtat cctggtagat atcatgataa ataacccggg ttctttgccg
     1081 cagtgtgtct taattatagg gcagttatta aatttcgaaa atgagagtaa ggctagagaa
     1141 atagttgaaa tgattataaa caaatatgct ttaaagccaa atactgaatt tatgatgata
     1201 tggtttcaaa gattgaccac catttttgga gaaatgtttg atagtgtttt atcatccgaa
     1261 tttgtatcta aaatcaaaga tcctgtctct aatcagatgt tttctgtaga ctggctaaat
     1321 gaagatgcta agaaatcttt ttatgaaggg accattaaag acgaagagaa atatattaaa
     1381 atgagtaaga aaatatatat tactgaattt gatgatttta atgattatcc tttttaagtg
     1441 aaaatttaga atgaatataa aaaacattac aatctaattt ttttaaaaaa atgttatatc
     1501 ggaagtaatc tcttgatttc tatactataa gataagaaaa ccactattta acaaggttat
     1561 tcgaacctta ttttaagtta cttattattt aatgtatcga aaaaggtgaa ataggtttgt
     1621 tataataaga attaagtaag acatgaattg tctaagcaaa tgtagcttat ccatttcatt
     1681 aaaatacata tggtgaaaaa acaagttcaa atagtaacta attttttaaa aaactgattt
     1741 ataaaaaata caacacgaat gaggaaaata accaatgaca gaacaaatcg aaaaaaattc
     1801 aaaaagctta tatcaggcac tgtggaattc cgcagatatc ctgcgttcaa aaatggacgc
     1861 taatgaatac aag
//
LOCUS       DACCCR010000161         1840 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCCR010000161 DACCCR010000000
VERSION     DACCCR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.176"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..609)
                     /locus_tag="IVK93_002742"
     CDS             complement(<1..609)
                     /locus_tag="IVK93_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387967.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPW"
     gene            650..1342
                     /locus_tag="IVK93_002743"
     CDS             650..1342
                     /locus_tag="IVK93_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6387968.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
BASE COUNT          563 a          347 c          327 g          603 t
ORIGIN      
        1 ccaaggatta aatgaaaacg tacttggcac gacgtgatct ggatcatagg tctcgatgat
       61 tttttcgtat tgttccttgt agtcattatc aacttttgct tgggggatca ctttacttgc
      121 ttcatgcaaa aagtgttctg aaccacgccc cattgcctgt ccccgaacag tatgtttatc
      181 caacgcaata tcaggaattt ctggaatata tcccatagcg aaactcttga taacaatgtt
      241 cttaagtaaa tccgatgaac gatctttttc agattggcat aggtaacgaa tcgcgtggaa
      301 aaaatacatc gcacgatcgg attcattgta ttggaattct ttgcgcatgg cgtttaagtt
      361 attgatcatg atggcggcca taggattgcc catgcccaca tcctcaacac tgatcgccaa
      421 tagccttctc cagagtttct cttcaaattg tggtgaggtg atatacattt cataagcaaa
      481 ttcacatgct tctttttctt ttcctcgacg aatcgatttc tgtaatgctg agatagcttc
      541 atctccacgt aagccgttac gggtagtcat ttttgaccac ggatcttgaa taaatttcgt
      601 ttctgtcata atataatact cctattagat aaaatttgag gtgaatctta tggatgttga
      661 aatgcttata gataaatacc aactgaatga ctcagaagct gaagtcttac gatttatgaa
      721 aaaaaatcgt cagtctttaa aaaatattgg gattcgtgac gttgccaaag catcgtatgt
      781 ttctactgca acgattatca atatggcgaa gaaaattgga tattccggtt atagtgagtt
      841 ggtatttgct ctaggaaaca gccaatctga cattacgatg ccaagcacat tctccaatga
      901 ggagaaggaa gcattcatac atttaatgtc tgcgtatcgc gataaacgga ttatgattct
      961 tggctcaggt ttttcgcaaa atctagccaa ttatttttcg gaatctctta atctatatgg
     1021 atttcgtgcg acggccaata gtcatttaga atttcttaga cagcaagtgc aacaagatat
     1081 tctgcttttt attatttcca attctggtga tacagttcga ttagccgaat tagctaaaat
     1141 tgcgaacgat cactttatcg atgtgatcgc atttgttggg caaaaaaatt caaaaatcgg
     1201 tacgctggca acattaacca ttagcacgga aacctatatt ccacaaaaaa tatctgatta
     1261 ccaaccaaat cttttctttg gcacgacact gaatcaattt gagcttttgt tgagcgcggc
     1321 gctgaaggct atttataaat agaagaacgc tgtggccact gtttcttcaa aatctagtat
     1381 aaagaatctt ctttagaatg taattgtttt ctgtgtcttc tgaacatgtt taggaatttc
     1441 caaacttgtt tagaaaactt tctcattccc tccgctgtag aattgaactc acataaaaaa
     1501 catctaaacc aaaatatcta caagcttgga aaggataata ggttgttatt aaaaataatt
     1561 tttattcctg aacctgaata attcatagct ttaattcttt ggcacatttt attgaaatat
     1621 gtatcactaa tatccctatc aactgatttt gactaaaaag ttacttcaaa cattcactgt
     1681 tcaaaaatat tgaggaataa attttgggtg taaggcattt tgagcatact tctccctgga
     1741 taacttccca gtaaaaaaat cgttagattg ttggattaga tccattgact gcctcgcctc
     1801 aggcgagcaa agaccctgta gaattcattc tgatacacat
//
LOCUS       DACCCR010000162         1748 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCCR010000162 DACCCR010000000
VERSION     DACCCR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.177"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(188..565)
                     /locus_tag="IVK93_002744"
     CDS             complement(188..565)
                     /locus_tag="IVK93_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387969.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(865..1746)
                     /gene="galU"
                     /locus_tag="IVK93_002745"
     CDS             complement(865..1746)
                     /gene="galU"
                     /locus_tag="IVK93_002745"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6387970.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          500 a          380 c          253 g          615 t
ORIGIN      
        1 attggtatta tttatatata tataaataac ataaaaaaag gaatggctta tcccgataca
       61 acaagcattc ctttaaaaaa gattctttta tataagtgaa aaaagctttt ttccaccaat
      121 taaataaatc tctcatctca atagtttatc cattatttta tctttttttg cgtataccta
      181 ctaaatatta ccgtcttctt ctcgttcttc tactttgttc gagagaatct gtactagaaa
      241 aagtagttgt atgtctacct tttctgccta tccgagactt tcttttcata tatgggatat
      301 aaatgattag atacagtaca gcaaaaaata aagcaaatac gcaagcttcc caactaccta
      361 acgccaattc aaccatcaag gtagcaaccc caacggatgc tgcataccag ataaaaatca
      421 gaaacgtttc tttttttctg accatgcgaa acatgataaa tagactaata agcaatagtg
      481 gcaacacaaa tatccaattg cttccttcca tcagccactt gagtatatca agcagcgact
      541 gtgcaacagc gactaatttt cccatagtga acacacacct ttttttataa ttaaaaaatt
      601 cgccttttta ttctactatg aatcttctgg cttggctttc atttctctta tgtaatcatt
      661 ttcttttata caacttaaaa aaaaattaaa ccattagcat aatattttct gaatctattc
      721 acgaaagtct ttttctatct ttcgttttcc acgactcaac tgatctagag tcaaaaaact
      781 aaaacgacag ttgcgcaaat gacgcacaag ctgccgcttt aataacaaat agcctttcat
      841 tctcagcagg ccacttattt ccagttaact ttctgttctt agctttttac tcaactggat
      901 aatatatgca cttaatgaag tcccaatttc cgaatgttga agtccatatt caatatttgt
      961 cttcatatat cctaacttat cgcctacgtc gtatcttttt cctttaaact ctctcgcata
     1021 gaccgcctct gtctggttaa gagtctgaag tgcatccgtt agctgtatct cgtttcctgc
     1081 tcctggttct tgagtttcta atatctcaaa tattttagga gtcagtagat aacgtccgat
     1141 aatggctaaa ttacttggtg cttttcccat ttcaggtttt tctacaaacc cttttactct
     1201 gcaacgaccc ttttcgctat ctgttttagg atcaataatt ccgtatttag atgtttcatt
     1261 ttctggtaca cgcatgacgg caacagcgga agcttgtgtt tcttcataat ccatcatcaa
     1321 ttgttgagtc aatgggatat catctttcat caaatcatcc cccagcatga cgacaaacgg
     1381 ttcatttcct ataaatgctt ttgcttgaag cacggcatcc cccagtcctc ttggatgaga
     1441 ttgacggatg aagtgaagat tgacttgagt cgtttcttca actaaagcga gtaattctgt
     1501 tttttccttt tcccgcaaat tgttttccaa ttcgatattt gcatcaaagt gatcttcaat
     1561 gggtcttttt tcttttcccg ttacgatcaa gatgtcttcg attcccgaat ccaatgcttc
     1621 ttccacaata aactggatcg ttggtttatc cacgattgga agcatttctt ttgccatggc
     1681 ttttgttgcc ggcaaaaaac gtgttcctag cccagcggca ggaatgactg cttttttgac
     1741 cttcactt
//
LOCUS       DACCCR010000163         1745 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCCR010000163 DACCCR010000000
VERSION     DACCCR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.178"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            131..505
                     /locus_tag="IVK93_002746"
     CDS             131..505
                     /locus_tag="IVK93_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6387971.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVK93_002747"
     CDS             complement(619..1248)
                     /locus_tag="IVK93_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6387972.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVK93_002748"
     tRNA            complement(1382..1454)
                     /locus_tag="IVK93_002748"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IVK93_002749"
     tRNA            1668..1741
                     /locus_tag="IVK93_002749"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
BASE COUNT          603 a          256 c          306 g          580 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaa
//
LOCUS       DACCCR010000164         1724 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCCR010000164 DACCCR010000000
VERSION     DACCCR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.179"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(366..1658)
                     /locus_tag="IVK93_002750"
                     /pseudo
     CDS             complement(366..1658)
                     /locus_tag="IVK93_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          535 a          353 c          294 g          542 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtgtacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcattgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1681 attgtagaaa aagaaaaacc gcgaagcaac cacctgccta ggat
//
LOCUS       DACCCR010000165         1629 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCCR010000165 DACCCR010000000
VERSION     DACCCR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.180"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            287..694
                     /locus_tag="IVK93_002751"
     CDS             287..694
                     /locus_tag="IVK93_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387973.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IVK93_002752"
     CDS             708..1109
                     /locus_tag="IVK93_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387974.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IVK93_002753"
     CDS             1111..1482
                     /locus_tag="IVK93_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387975.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          612 a          209 c          283 g          525 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtataca
//
LOCUS       DACCCR010000166         1608 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCCR010000166 DACCCR010000000
VERSION     DACCCR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.181"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IVK93_002754"
     CDS             complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IVK93_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP6387976.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEHHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            750..1608
                     /locus_tag="IVK93_002755"
                     /pseudo
     CDS             750..1608
                     /locus_tag="IVK93_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          522 a          306 c          306 g          474 t
ORIGIN      
        1 ggggattttt aaaagaccgc caactgctca tcttcactca cttacttctc tttatttttt
       61 aaatggttct acgtactctc ttttacttat aatatatcga atgcgatcat cagcttcttg
      121 ttcacggatg tatttttcat ttgtcttttg attcaatccg actgtactca catagtatct
      181 cttcgaccaa aaggatttgt ttccgtattt atatttgaga tttgcgtggt gctcgtggat
      241 tagtaccgca ctacgacctt tcagataccc cataaaactg gatatacata tttttggtgg
      301 tactcgaaca agcatgtgaa tatgatctag cattgcatgt gcttctatga tttccacatc
      361 tttcatttcg cacaacttcc taagtatttt tccaagatct tttcgtaatt gcccatagat
      421 tacttttcgt tgatattttg gtgtaaaaat aatatgatac ttacaattcc aacgagtata
      481 tgctaaactt ttatcgtcat ttgacatgaa aaaactcctt tcgatctgat gagcggttgg
      541 tagccactca ctagtatatc gaaaggagtt tttatcgcta aagatacttc ttccacgcgt
      601 agaaacgcgc ggttttcttg tttcgcacgc tttacgcaca aaactgactg aagtcattaa
      661 gaaaataaaa gggtctaaaa cttttaagtt ttgtaccgac ccccaaaagt tagaccaaaa
      721 aatctaactt ttggaggtcg gtatttttta tggctaagta tacttttgaa cttaaattaa
      781 agatcgttca tgattacttg gatggtaaag gcggaagtga ttatctggca aagaaatatt
      841 ccattaaggc tccatcacaa gtgaagcgct ggatcaatgc ctatcaagaa tttggtgaag
      901 agggtctagt aagaaagcga caaaagaaaa aatattctgt tcaatttaag ctggatgcga
      961 tagagttata tctaacaagt gagctgtcct atcgtgaagt tgccaacaca cttaacatga
     1021 acaaccctaa tttgatcgct agttggatgc gtcaattccg tgagggtggg atcgatggac
     1081 tctcaaaaac gaaaggatgt ccacctatct tgtctaagaa aaatgaaccc aaaaataaga
     1141 aaaaatctac aactaaagca acgtctaaag aacgtgaacg catcgaggag ttggagaaac
     1201 gagtccgttc ccttcaaatt gaaaatgcct ttttaaaaga attgaggaag ctgcgaaaac
     1261 aggaagccca acaacgacga atgaagcaat cgcacgaatc atcgcaagcc tccgaggacc
     1321 gtttaaatta gttgagcttc tagaaacttt aaagttccct aaggcaatct ttatgtactg
     1381 gcaaaaacga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaagt
     1441 tcgggagaag cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg
     1501 tttctctatc aataaaaaga aagttcagcg cctgatcaag aagctaggaa taaaagtaac
     1561 gacatacaca cgtaaatcac ggcgctacaa ttcttatcga ggacaagt
//
LOCUS       DACCCR010000167         1563 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCCR010000167 DACCCR010000000
VERSION     DACCCR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.182"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            66..149
                     /locus_tag="IVK93_002756"
     CDS             66..149
                     /locus_tag="IVK93_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP6387977.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            274..1011
                     /gene="erm(B)"
                     /locus_tag="IVK93_002757"
     CDS             274..1011
                     /gene="erm(B)"
                     /locus_tag="IVK93_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6387978.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1181..1441
                     /locus_tag="IVK93_002758"
                     /pseudo
     CDS             1181..1441
                     /locus_tag="IVK93_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
BASE COUNT          589 a          249 c          268 g          457 t
ORIGIN      
        1 tgtataatag gaattgaagt taaattagat gctaaaaatt tgtaattaag aaggagggat
       61 tcgtcatgtt ggtattccaa atgcgtaatg tagataaaac atctactgtt ttgaaacaga
      121 ctaaaaacag tgattacgca gataaataaa tacgttagat taattcctac cagtgactaa
      181 tcttatgact ttttaaacag ataactaaaa ttacaaacaa atcgtttaac ttctgtattt
      241 atttacagat gtaataactt caggagtgat tacatgaaca aaaatataaa atattctcaa
      301 aactttttaa cgagtgaaaa agtactcaac caaataataa aacaattgaa tttaaaagaa
      361 accgataccg tttacgaaat tggaacaggt aaagggcatt taacgacgaa actggctaaa
      421 ataagtaaac aggtaacgtc tattgaatta gacagtcatc tattcaactt atcgtcagaa
      481 aaattaaaac tgaacattcg tgtcacttta attcaccaag atattctaca gtttcaattc
      541 cctaacaaac agaggtataa aattgttggg agtattcctt accatttaag cacacaaatt
      601 attaaaaaag tggtttttga aagccatgcg tctgacatct atctgattgt tgaagaagga
      661 ttctacaagc gtaccttgga tattcaccga acactagggt tgctcttgca cactcaagtc
      721 tcgattcagc aattgcttaa gctgccagcg gaatgctttc atcctaaacc aaaagtaaac
      781 agtgtcttaa taaaacttac ccgccatacc acagatgttc cagataaata ttggaagcta
      841 tatacgtact ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat
      901 cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt tacttatgag
      961 caagtattgt ctatttttaa tagttatcta ttatttaacg ggaggaaata attctatgag
     1021 tcgctttttt aaatttggaa agttacacgt tactaaaggg aatggagata aattattaga
     1081 tatactactg acagcttcca agaagctaaa gaggtcccta gcgcctacgg ggaatttgta
     1141 tcgataaggg gtacaaattc ccactaagcg ctcgggaccc cttgtaggaa aatgtcctaa
     1201 gtgtggcaac aatattgtat taaaaaaatc gttttatggt tgttcaaatt atcctgaatg
     1261 taagtttact ttagctgaac attttagaaa gaaaaaacta accaaaacga atgtaaaaga
     1321 attactggag ggaaaagaaa ccctggtaaa aggaatcaaa aacaaagaga aaaagcccta
     1381 caatgccgtt gtaaaaattg gggaaaaggg atatattgat tttatctctt tttcaaaata
     1441 aacatggggc tgacccaaaa gtagctccta tatgaaaaat agggctcttt gtcaataagg
     1501 actgatgagt tgtgcaaaat caaatctgag tcagaatgaa ccacattctg gctcagattt
     1561 ttt
//
LOCUS       DACCCR010000168         1534 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCCR010000168 DACCCR010000000
VERSION     DACCCR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.184"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            96..506
                     /locus_tag="IVK93_002759"
     CDS             96..506
                     /locus_tag="IVK93_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6387979.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            520..876
                     /locus_tag="IVK93_002760"
     CDS             520..876
                     /locus_tag="IVK93_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6387980.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          564 a          227 c          281 g          462 t
ORIGIN      
        1 ttattttgat ttttttttat attttattag ctaataattg gtattttaaa agaaattact
       61 gtagaattgg attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta
      121 gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag
      181 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagacc ttggcagcga
      241 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
      301 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat
      361 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
      421 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
      481 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
      541 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
      601 tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc
      661 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
      721 attgcgattg acctttcctg tgtagaaaaa aaacaactga tttttttatg cacacaacat
      781 accaacctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
      841 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
      901 ctgagcattc catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct
      961 ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa
     1021 tctaggtggg gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc
     1081 atttctgttg ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag
     1141 ttgttgacat gttagaaatg acatgataag ataactgagt cgttaaaaaa ggaccgcttg
     1201 cgaagcttga ttctatcgaa aaaacttcaa aaaagttctt gacaaataac aaacgacaag
     1261 ttatgataaa tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa
     1321 gagttgacaa ttacttgtct ctctgatatg atataaaagt tgctgcggca gccaaaaacg
     1381 aatcgggaaa cactcctgat gaagtagacc tttgaaaaat gaacaaagta agacaaacca
     1441 aatgtgtagg gcgtcttgat tcaattcaag acaacaaaca tttttaacaa gcaagcaata
     1501 tgctagcaaa caaattgagc ttaacaatcg caag
//
LOCUS       DACCCR010000169         1533 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCCR010000169 DACCCR010000000
VERSION     DACCCR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.185"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(177..1196)
                     /locus_tag="IVK93_002761"
     CDS             complement(177..1196)
                     /locus_tag="IVK93_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6387981.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1207..1413)
                     /locus_tag="IVK93_002762"
     CDS             complement(1207..1413)
                     /locus_tag="IVK93_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP6387982.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          431 a          318 c          261 g          523 t
ORIGIN      
        1 cataaagcca taaacaatca accagtagaa tgtgtggcaa caaacctgtt atcgcatagc
       61 ttggagtgtg actattatgg gtgatagtga agatatgcga taacatcact attttatcga
      121 atgattttta tagttgtcaa tatagttatg taatgctcct caacgaggag ctatttttat
      181 cgtttaggaa tatttaaata ccaacgtttg tcatggaaat cttgcgcacc gcctttagtg
      241 tttccctctg gatcattcgt tgcccgcatc atgacataga ctttcttatt agggaagtta
      301 cgcatgttaa aagatacatg atagccaaca tttccagaag tattataagc ttgatttaca
      361 tctgatctat aaattccatc agctcttact cgagctaatt ctttcccagt attgtaatcc
      421 ataatgaaga tatactcgta tttatagtta gcaatgtgcc atccagctac atgcaaattt
      481 gcattttcga tttctccaaa ctgatcaatg tgggcgtaat tcgttccatc tgtcagtgta
      541 ggattagctg caccagctcg tgttggatca atgacaggct tgttttcaga agttgttgga
      601 ttttcatcgg taaatccatg agctaaatca tatgctaatt tttctttact tacgcccatt
      661 tcagaaagat aaccgtaagg atctgtatga tcgccccaga tgttttgtgt tacccataaa
      721 tgcgatttga ttcccggttg gttataagga gtgtccaacg ttaatggaat accatatttc
      781 attgctgaat ctctagccaa ttcaacgtat gccttgtagt ttttctcaaa cgttgcttta
      841 tcatgtgtgt gttgtaactc aatctgcaca ggactgttgg cattagcata cgaaccagca
      901 ccgtactgta cataaccagg ttgaccgact tgataaacaa ttccgccgtc tcccacaata
      961 taagcagtat aagcgctagt ccatgaacgt tgcatatact gcgcttcatt gcgtcctgtt
     1021 gctgtttcat tagccgtttc atgcagtaaa atatactgat tatttgctac ttgtgagcta
     1081 ccttcatttg cgcccaaatt aaattcattg ttgatagtat aggcaaatcc gttaattggc
     1141 aataaaaaaa gagccgttaa caggctcatc gcagtaatag taattttctt tttcatatct
     1201 atcctcctat ttatctgatg aaattttctt atacgttcga tttgatacac ctaaaatact
     1261 ccctagaaat gctccaaaac ctgtaataat catcaccgtt atatctgtgt accgccagtt
     1321 aacacctttt ccgacaagac cgacaaaagt cgctaaagcg gggataacta ctatagcgat
     1381 ccatttcaaa atatcaaagg ttctattgtt catactatcc acctcccttc attggttggg
     1441 ctactttctt tttcaatcgc ttgttttctt ctatcaatat ttctctttct gaaagcaagg
     1501 catcaatttg cccgtccagt tgttcaattt tat
//
LOCUS       DACCCR010000170         1522 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCCR010000170 DACCCR010000000
VERSION     DACCCR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.186"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            161..271
                     /locus_tag="IVK93_002763"
                     /pseudo
     CDS             161..271
                     /locus_tag="IVK93_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(368..844)
                     /locus_tag="IVK93_002764"
     CDS             complement(368..844)
                     /locus_tag="IVK93_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6387983.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(857..>1522)
                     /locus_tag="IVK93_002765"
     CDS             complement(857..>1522)
                     /locus_tag="IVK93_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6387984.1"
                     /translation="MYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWL
                     YVIHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWA
                     FIYFFVFTFFIKKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIES
                     VEACATRLRVAVTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILG
                     KDN"
BASE COUNT          528 a          278 c          196 g          520 t
ORIGIN      
        1 catctttcag cattttacta tatttggtat atagtgctaa ggttcatatt cgattgacaa
       61 gagaatataa attgatctat ttctatttcc taagttgagt aaacataact ttgcccttta
      121 taaatatctt ccctctctct cttctctcta tattttatat ttgaataatc atatcaaggt
      181 attccaacga aatgccaatg gatttcgaag cttttataac tttaaattac ggatcatgat
      241 ttggcatggg gaatcattac taataaaata aaaaaatgaa cagaaagaaa acagttcttt
      301 ctgttcacca acttcatttg acaaatatcc tatttagctt attttatagc tttttatata
      361 cactttttta aaaagtgatt tttccacatg aattcccatg agcaattcga ccattatcta
      421 ttattaaatc attgaactga tttttctccg gaaaaacaac tataacagaa gtatctttac
      481 cagattttat tattttgtca aaatccacat caaccaacga ggtttcagga gtaacctcat
      541 cattttcttg aacacaaatc gaaaaaggct ctccttgtaa ttcaactgta tcaattccaa
      601 tatgaatcaa ataatcaatg cctgagttgc tttttatatt aatagcatgt ttagtaggaa
      661 atatatttgt tactcgacca gccaatggag cataaatttt tgaaccatca ggtatgacgg
      721 caaaaccatc acctagtaat ttttgtgaaa agacaggatc atttacttct gtgatggaaa
      781 tgatttttcc ggaacaaggt gcataaaaag tttcttgttt tttctttttg aaaatttcaa
      841 acatattaaa aacctcctaa ttatcttttc ctaatatttg attgatttct tgacttaata
      901 agtcagcttt ccctccaaaa acagcttgga tgccaccctt cacttcgaag acagcagttg
      961 caccagtgtc ttttattgtt tgtttttgaa taatctcccc attcgtaaca gcaacacgca
     1021 aacgagttgc acaagcttca acagactcaa tattttcttt tccaccaaga gcttcaataa
     1081 tgataagaga ctcttcatgt aatgatgtat ttttgtttat tactttttca gaatgaactt
     1141 ctgattcttc ttccattcca ggaatagcta ccttaaattt tttgataaag aatgtaaaaa
     1201 caaagaaata tataaatgcc catgcaatac caaaaggaat taattttaac caattagttt
     1261 tgtcattccc ttgaagtaca ccgaataata aaaagtcgat caaaccacct gaaaaggaat
     1321 tccctacacg aatatttaag atatcagcaa aataaaaaga taatccatct aagaatgcat
     1381 gaatcacata aagccaagga gcaacaaata aaaacatata ttcaactggt tcagtaattc
     1441 cagttaaaaa tgatgtaagg gccccgctaa aataaagccc accattcttt ttacgatttt
     1501 tcttaggaat tgcacgatac at
//
LOCUS       DACCCR010000171         1456 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCCR010000171 DACCCR010000000
VERSION     DACCCR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.187"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            108..1040
                     /locus_tag="IVK93_002766"
     CDS             108..1040
                     /locus_tag="IVK93_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP6387985.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IVK93_002767"
     CDS             1112..>1456
                     /locus_tag="IVK93_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387986.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACCCR010000172         1440 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCCR010000172 DACCCR010000000
VERSION     DACCCR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.188"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            105..1406
                     /locus_tag="IVK93_002768"
     CDS             105..1406
                     /locus_tag="IVK93_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6387987.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DACCCR010000173         1425 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCCR010000173 DACCCR010000000
VERSION     DACCCR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.189"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(74..158)
                     /locus_tag="IVK93_002769"
     tRNA            complement(74..158)
                     /locus_tag="IVK93_002769"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(122..124),aa:Leu,seq:caa)
     gene            complement(176..247)
                     /locus_tag="IVK93_002770"
     tRNA            complement(176..247)
                     /locus_tag="IVK93_002770"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Gln,seq:ttg)
     gene            complement(257..329)
                     /locus_tag="IVK93_002771"
     tRNA            complement(257..329)
                     /locus_tag="IVK93_002771"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(294..296),aa:His,seq:gtg)
     gene            complement(391..475)
                     /locus_tag="IVK93_002772"
     tRNA            complement(391..475)
                     /locus_tag="IVK93_002772"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Leu,seq:caa)
     gene            complement(496..566)
                     /locus_tag="IVK93_002773"
     tRNA            complement(496..566)
                     /locus_tag="IVK93_002773"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(532..534),aa:Cys,seq:gca)
     gene            complement(612..683)
                     /locus_tag="IVK93_002774"
     tRNA            complement(612..683)
                     /locus_tag="IVK93_002774"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(649..651),aa:Gln,seq:ttg)
     gene            complement(695..767)
                     /locus_tag="IVK93_002775"
     tRNA            complement(695..767)
                     /locus_tag="IVK93_002775"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(732..734),aa:His,seq:gtg)
     gene            complement(770..843)
                     /locus_tag="IVK93_002776"
     tRNA            complement(770..843)
                     /locus_tag="IVK93_002776"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(809..811),aa:Trp,seq:cca)
     gene            complement(850..930)
                     /locus_tag="IVK93_002777"
     tRNA            complement(850..930)
                     /locus_tag="IVK93_002777"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(894..896),aa:Tyr,seq:gta)
     gene            complement(932..1007)
                     /locus_tag="IVK93_002778"
     tRNA            complement(932..1007)
                     /locus_tag="IVK93_002778"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(972..974),aa:Phe,seq:gaa)
     gene            complement(1043..1115)
                     /locus_tag="IVK93_002779"
     tRNA            complement(1043..1115)
                     /locus_tag="IVK93_002779"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1080..1082),aa:Asp,seq:gtc)
     gene            complement(1125..1197)
                     /locus_tag="IVK93_002780"
     tRNA            complement(1125..1197)
                     /locus_tag="IVK93_002780"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1162..1164),aa:Val,seq:tac)
     gene            complement(1213..1284)
                     /locus_tag="IVK93_002781"
     tRNA            complement(1213..1284)
                     /locus_tag="IVK93_002781"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1249..1251),aa:Glu,seq:ttc)
     gene            complement(1299..1388)
                     /locus_tag="IVK93_002782"
     tRNA            complement(1299..1388)
                     /locus_tag="IVK93_002782"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1352..1354),aa:Ser,seq:gga)
BASE COUNT          412 a          367 c          324 g          322 t
ORIGIN      
        1 atttggaggt aattacataa aaacaaaaaa aatatagcaa ctctctataa gaaagttgct
       61 atattgataa ttataccggc ggccggggtc gaaccggcac gtcctcaacg gacactggat
      121 tttgagtcca gcgcgtctgc caattccgcc acgccggcaa atttaaaaat aataactggg
      181 gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta
      241 taccccaaga ataaagggcg aatgatggga atcgaaccca cgaatgccgg agccacaatc
      301 cggtgcgtta accacttcgc cacattcgcc atcataaaag atgttattta atttctcaat
      361 aaaaaactaa gatgaagaca tcttagtcaa taccggcggc cggggtcgaa ccggcacgcc
      421 ctcaacgggc actggatttt gagtccagcg cgtctgccaa ttccgccacg ccggcaagtt
      481 gatttattag tactaaggcg gtaaccggat ttgaaccggt gatgaaggtt ttgcagacct
      541 ctgccttacc acttggctat accgccatta tactattatc aatctataaa ataaagaata
      601 aattatagaa actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg
      661 ccttaccgct tggctatacc ccaagaataa aaagggcgaa tgatgggaat cgaacccacg
      721 aatgccggag ccacaatccg gtgcgttaac cacttcgcca cattcgccat ggcaggggca
      781 gcaggaattg aacccacact aacggttttg gagaccgctg ttctaccttt aaactatgcc
      841 cctataaaat ggaggagagt ggattcgaac cactgaaccc taaggaacgg atttacagtc
      901 cgtcgcgttt agccacttcg ctactcctcc atggtggcgc gggacagaat cgaactgccg
      961 acacacggag cttcaatccg ttgctctacc aactgagcta ccgcgccata ttttaaaatt
     1021 attaattgaa taacaagaac gacggttccg acgggaatcg aacccgcgat ctcctgcgtg
     1081 acaggcaggc atgttaaccc ctacaccacg gaaccagttc attatggagg ttaacgggat
     1141 cgaaccgctg accctctgct tgtaaggcag atgctctccc agctgagcta aacctccaac
     1201 attcaattaa aatgacccgt acgggattcg aacccgtgtt accgccgtga aagggcggtg
     1261 tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg gagagtaagg gattcgaacc
     1321 cttgagacag tttggcacca tctacatgat ttccaatcat gctccttcgg cctctcggac
     1381 aactctccag agtgaaagaa gctcaatact ctttctcaaa atgag
//
LOCUS       DACCCR010000174         1424 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCCR010000174 DACCCR010000000
VERSION     DACCCR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.190"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(150..1337)
                     /locus_tag="IVK93_002783"
     CDS             complement(150..1337)
                     /locus_tag="IVK93_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP6387988.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACCCR010000175         1393 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCCR010000175 DACCCR010000000
VERSION     DACCCR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.191"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            96..1391
                     /locus_tag="IVK93_002784"
     CDS             96..1391
                     /locus_tag="IVK93_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6387989.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          442 a          277 c          260 g          414 t
ORIGIN      
        1 cttcaccaac atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga
       61 attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag
      121 ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa
      181 aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt
      241 gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc
      301 ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat
      361 ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact
      421 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
      481 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
      541 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
      601 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa
      661 gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt
      721 ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc
      781 cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc
      841 acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac
      901 gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga
      961 aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt
     1021 ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact
     1081 tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt
     1141 cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca
     1201 ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt
     1261 caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg
     1321 gctttcgcaa cttttacaat ttcaaactac gaattatgat tcgtcatggc aaaacatttt
     1381 taaccaaata aac
//
LOCUS       DACCCR010000176         1324 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCCR010000176 DACCCR010000000
VERSION     DACCCR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.192"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(82..636)
                     /locus_tag="IVK93_002785"
     CDS             complement(82..636)
                     /locus_tag="IVK93_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6387990.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          448 a          227 c          141 g          508 t
ORIGIN      
        1 acttccgaaa attcagttat cttcattaat ctcaccttct aagttttcat tatacacaaa
       61 aaatatatcc taatatctca attagcctcc caatttaaaa ccacgtaacg atgcccctat
      121 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
      181 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
      241 taatgtatgt gttcctatat catctcttcc taatttttct gctatatctg catatatctg
      301 atagacacga tcccttgtta tatgtcgacc ttgttgccga cttggaaata accaatcatt
      361 ttcttcttta ccttcagtat ataataaaat ctcttctctc aaattatcta taaaaatcgg
      421 ttttcgctta cctgttttat gttcaacaat aataggctta cttttacctc gtatgtctcc
      481 aacttttaat cctataatat ctgacatacg taacccatta ttgattccaa acaaaaataa
      541 aaaactatct ctttctggat tacttgttct tcttagccaa aactgaaaat cttgtatctc
      601 ttcatttgtc cttaacggtt gtacattata gctcactatt gtttcccttc ttaaaaatat
      661 tctgagttca tgattatata tatattttac cataatcatg aacttatata tatactctgt
      721 taaaagtgaa gaaaatcaag gggtttagtt tatgatttta tatcatttca gtaactcaga
      781 atattccgac ttttttgtac aaaaaaatta accttactta tatgaagatt ctaacctctt
      841 atttaaattt tagacgggca attatccgta gaagttctta gaatagctaa tttacataat
      901 taaattatat gaaatttaag tttgaacaat atactaagaa ctccccttac tataaagtga
      961 aataaattag catatgaata accgtgctac caagcttttc attaataaaa tatttttatt
     1021 gatattttat aactttacgc aagatcataa ttttgtctgt gtagatattt cgttatatca
     1081 ctaaagataa taagatttaa tgacctcatt aatattgttt gctagatcca aactcttatt
     1141 aaactttcct tgctataaaa cccaaacata gtaataaaag aataacttat tacctttaaa
     1201 ttatactacc aacaaatggt agtatattat taatttatat aatatgaaac ctaaaatata
     1261 gtagtatacc cgtggtgcta gttaattttt tcctagagga aaatgtctaa acatgttggt
     1321 aaat
//
LOCUS       DACCCR010000177         1311 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCCR010000177 DACCCR010000000
VERSION     DACCCR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.193"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            200..1248
                     /locus_tag="IVK93_002786"
                     /pseudo
     CDS             200..1248
                     /locus_tag="IVK93_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          255 g          376 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DACCCR010000178         1228 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCCR010000178 DACCCR010000000
VERSION     DACCCR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.194"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>1228
                     /locus_tag="IVK93_002787"
     CDS             <1..>1228
                     /locus_tag="IVK93_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP6387991.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQ"
BASE COUNT          405 a          219 c          267 g          337 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaaca
//
LOCUS       DACCCR010000179         1215 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCCR010000179 DACCCR010000000
VERSION     DACCCR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.195"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            414..947
                     /locus_tag="IVK93_002788"
     CDS             414..947
                     /locus_tag="IVK93_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP6387992.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACCCR010000180         1132 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCCR010000180 DACCCR010000000
VERSION     DACCCR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.196"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(176..>1132)
                     /locus_tag="IVK93_002789"
     CDS             complement(176..>1132)
                     /locus_tag="IVK93_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6387993.1"
                     /translation="DRYGDRVKNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNY
                     HKVLATAKAVEIYRSGNYGGRIGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDP
                     MVKGYYTEEFLELCQKQSIYFDPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNE
                     ETPFHPERYYEVFDLPGKKMNNSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGR
                     EHEERYMDEKGIVQDDYRIEFIGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFK
                     NRYGLVRIELNDERSRTLKKSASWYQTLIDKRELDLPDEIYK"
BASE COUNT          331 a          242 c          174 g          385 t
ORIGIN      
        1 cgaataaacg gtattcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca
       61 taagatgttg tttttacttt gctattctta ttacttgatg caggacagct ttttcacaac
      121 ctcttttatt tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt
      181 tatagatttc gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag
      241 cagatttttt caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc
      301 gattcttaaa tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt
      361 cgcaatttgc gccttcttct gttgctttga tcaaataacg caaatgctgt ccgataaatt
      421 cgatacggta atcatcttgc acgattcctt tctcatccat ataccgttct tcatgttctc
      481 ttcccatacc gttttctgtt atcagccaag ggatattatg ataattttct tttagataca
      541 tggctatatc atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct
      601 tcccaggtaa atcaaagact tcataatatc gttctggatg gaaaggtgtc tcttcattcc
      661 attgataacg aggagcgcga acgcgtttag gataatattg attgattcca aggaaatcta
      721 ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt
      781 tttgacacaa ctccaggaac tcttctgtgt agtacccttt taccatcgga tcaaaaaata
      841 ctcgattata aaacaaatca tacatataag cagctttttg gtcttctgga gaattagatc
      901 gagcatagac catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc
      961 ggtaaatctc tactgcttta gccgttgcta aaactttatg ataattccac atcatccact
     1021 tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa
     1081 cgattggttc gttgaatgta aaccagtttt ttaccctatc accgtaacga tc
//
LOCUS       DACCCR010000181         1119 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCCR010000181 DACCCR010000000
VERSION     DACCCR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.197"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>1119
                     /locus_tag="IVK93_002790"
     CDS             <1..>1119
                     /locus_tag="IVK93_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP6387994.1"
                     /translation="NLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKELASQS
                     SLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQTNYN
                     THYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPNTDIL
                     VRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREVQYFS
                     TTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGTMENF
                     IKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHSIRLK
                     FLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRR"
BASE COUNT          348 a          228 c          216 g          327 t
ORIGIN      
        1 acaacttatc catacttgag cagttcatta tgcaattaat tgctggttac tcagctgatt
       61 ctgcagctaa tctcctgaga caagatcctg tgtttaaagc tgttttagat aggaaagaac
      121 tcgcttctca atcttcactt tcccgatttt tagatcgact atctgaggag aatatccatg
      181 aacttcaagc tttgaaccaa gaacttatcg ataaagcacg cctcatccgt aatgatacgg
      241 aattaatcat tgatttagat tcgacccatt cagatacatt tggtcaccaa gaacaaacga
      301 attataatac acactaccaa acctatggtt accatccatt ggtagctttt gacggattga
      361 ctggtgactt cttaaaagct gaactacgtt caggaaatca atatacatca aaaggcgtaa
      421 aggagtttct cacaccttta ttagagcact ataatcactc tctaccaaac actgacatct
      481 tggttcgtgg agacagcggg ttcgctacac ctggtgtgta tgattcgtgt gaatcaaaaa
      541 agagtcatta tgttattcga ctgaagaata atcgtagact aggtcaaata gctgagaaat
      601 cagtacttta tggcgataat caaaagtggg aagaacgaga agttcagtac ttctcgacca
      661 cttatcaagc acaatcctgg tcacaaagtc gtcgcgtgtg tatacgctca acacgtgaag
      721 cgggcgaact actctttcga catgaattta tcgtgacgaa tctatcagaa aatgtttctc
      781 ctgataccat cttttctatc tatgccaaac gtggcacaat ggagaacttc attaaagaag
      841 cgaaagctgg cttttacttt gacaaaacag acagtcctcg ctttttggag aatcatgtcc
      901 gaatgatgct cagcttgata gcttacaact tagtcaactt cttaagaacg attggctttg
      961 aagaagtcca aaagggaatg accattcatt ctattcgatt gaagtttctg aaagttgctg
     1021 ggaaattagt ccaaacgggt agacgagtct atctcaaatt atctagttat catgtgtatc
     1081 agaatgaatt ctacagggtc tttgctcgcc tgaggcgag
//
LOCUS       DACCCR010000182         1080 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCCR010000182 DACCCR010000000
VERSION     DACCCR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.198"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(126..>1080)
                     /locus_tag="IVK93_002791"
     CDS             complement(126..>1080)
                     /locus_tag="IVK93_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAP6387995.1"
                     /translation="VLNEHGLESVNNEYYENLEKRTNLYSNDPSKMKYGKINYQEFVQ
                     NAIEATLEKCEVNSFEDFSDHLAKEYNIEVYQFSKTNNKLGYVLYKQGVNFYERRGEL
                     ITLGRDKQSRKEKSQYVERTFSARKLGKRYSFESVSEQLEKNLEESHIKNFFNMEREQ
                     VMSLISGVNYKTGELNVPFVELDSIAQKQFSDREGDEKFIKYDDFLREVDKHFVNTMS
                     LEDDQEYTIKLHTDVNPQTLRSAKSKTISIKKTDQKELFLPAKGFENCSHTPSAKQIH
                     YQISRIMNDDVKMYRAKKDYEFTQQMIENQQKRQQNFEMEF"
BASE COUNT          311 a          203 c          157 g          409 t
ORIGIN      
        1 agcctagcgt gagcggggcg gattgactgg caagtagatg ggtagctggc gtatgtccta
       61 aaaactgctt ctttccaagc aactcttcat aggcaaaaaa ctggaaagtc cactaacaag
      121 cccattcaga attccatttc aaaattttgt tgcctttttt gctgattttc aatcatttgt
      181 tgtgtgaatt cataatcctt tttagcacga tacattttta catcgtcatt cattatgcga
      241 ctgatttgat agtgaatctg tttagcagaa ggtgtatgag aacaattttc aaaacccttt
      301 gccggtagaa ataactcttt ctgatcagtc tttttaatag aaattgtttt acttttcgca
      361 ctacgtaaag tttgaggatt cacatccgta tgaagtttaa tcgtgtattc ttgatcatct
      421 tctaaagaca ttgtatttac aaaatgcttg tctacctctc gcaagaagtc atcatattta
      481 atgaattttt catcgccttc acggtcgcta aattgctttt gtgcgatgga atctaattcc
      541 acaaacggta catttaactc accagttttg taatttactc cactaattag cgacataact
      601 tgctctcttt ccatattaaa aaaatttttt atatgtgatt cttctaaatt cttttcaagt
      661 tgttcagaaa cagattcaaa tgaatatctt ttccctaact ttcttgcact aaaagtacgt
      721 tcgacatact gacttttttc ttttctagat tgtttatctc ttcctaaagt aattaactca
      781 cctctacgct catagaaatt tacaccttgt ttgtaaagaa catagccaag tttgttattt
      841 gtttttgaaa attgataaac ttcaatgtta tattcttttg ccaaatgatc cgaaaaatct
      901 tcaaaagaat tcacttcgca tttttccaac gttgcttcga tcgcattttg cacaaactct
      961 tgataattaa tttttccata tttcatctta gaaggatcgt ttgaataaag attggttctc
     1021 ttttctaagt tttcatagta ctcattattc actgattcaa gaccatgttc attgaggact
//
LOCUS       DACCCR010000183         1058 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCCR010000183 DACCCR010000000
VERSION     DACCCR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.199"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(36..992)
                     /locus_tag="IVK93_002792"
     CDS             complement(36..992)
                     /locus_tag="IVK93_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6387996.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACCCR010000184         1035 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCCR010000184 DACCCR010000000
VERSION     DACCCR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.200"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>1035)
                     /locus_tag="IVK93_002793"
     rRNA            complement(<1..>1035)
                     /locus_tag="IVK93_002793"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACCCR010000185          999 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCCR010000185 DACCCR010000000
VERSION     DACCCR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.201"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>999
                     /locus_tag="IVK93_002794"
     CDS             <1..>999
                     /locus_tag="IVK93_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP6387997.1"
                     /translation="NQLMENQRTEYIQAKEYERTENRQSQRNGYYERSFTTRVGTLEL
                     KVPRTRDGHFSPTVFERYQRNEKALMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSF
                     VSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVREENRVLSKSCHIAIGITKDG
                     DREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQ
                     RCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKA
                     CASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRL"
BASE COUNT          369 a          194 c          181 g          255 t
ORIGIN      
        1 taatcaacta atggaaaatc aacgaacaga atatattcaa gcaaaagaat atgaacgaac
       61 agaaaaccga caaagtcaac gaaatggcta ttatgagcgc agctttacga cacgtgtagg
      121 cacgctagaa ttaaaagtac ccagaacacg tgatggccat ttttcaccca cagtgtttga
      181 acgttatcaa cgaaacgaaa aagccctcat ggcttcaatg ttggaaatgt atgtatcagg
      241 cgtttcaact cgtaaagtat caaaaattgt ggaagaactt tgtggtaaat ccgtctctaa
      301 gtccttcgtt tctagcttaa cagaacagct agaacctatg gttaacgagt ggcagaatcg
      361 tttattatca gaaaaaaatt atccttactt aatgaccgat gtactctata taaaagtacg
      421 agaagaaaat cgagtactct caaaaagctg tcatatagcg attggaataa ccaaagatgg
      481 cgaccgtgaa attatcggct tcatgattca aagtggcgaa agcgaagaga cctggacaac
      541 attttttgaa tacctaaaag aacgcggttt acaaggtacg gaactcgtta tttctgatgc
      601 gcacaaagga ttagtctctg ccattagaaa atccttcacc aacgtaagtt ggcaaagatg
      661 ccaagttcac ttcctaagaa atatctttac caccattcct aaaaaaaatt caaaatcttt
      721 cagagaagct gttaaaggaa tttttaagtt cacagatatt aacttagcgc gtgaggctaa
      781 aaatcgattg attcatgatt atatcgatca accaaaatat tcaaaagctt gcgcatcatt
      841 ggatgatgga ttcgaagacg cctttcaata taccgtacaa ggaaattccc acaatcgact
      901 aaagagtacc aatctaattg aacgactgaa tcaagaagta cgcagaagag aaaagattat
      961 tcgcatcttc cccaatcaaa catcagccaa tcgcttaat
//
LOCUS       DACCCR010000186          970 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCCR010000186 DACCCR010000000
VERSION     DACCCR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.202"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            82..>970
                     /locus_tag="IVK93_002795"
     CDS             82..>970
                     /locus_tag="IVK93_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP6387998.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANIN"
BASE COUNT          329 a          182 c          191 g          268 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc
//
LOCUS       DACCCR010000187          951 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCCR010000187 DACCCR010000000
VERSION     DACCCR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.203"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            28..>951
                     /locus_tag="IVK93_002796"
     CDS             28..>951
                     /locus_tag="IVK93_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6387999.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLS"
BASE COUNT          335 a          175 c          181 g          260 t
ORIGIN      
        1 cgaaagaatt ataaggagtg agtacaaatg acctataccc atcttacacc aactgaactc
       61 gtaatgatag aagcttattt caatcaatca cactctgttt caaaagtagc ccatctggta
      121 caacgttctc gacaaaccat ttataatgtc tatcgttttc ttaaatctgg tggaagcgca
      181 ataagctact atgaacaata taagaaaaac aaacgtaact gcggaagacg tccgattgtg
      241 ttaccagatg ggcaacaaga atacattcaa aaaaaggtgg ctcaagggtg gactccagat
      301 gtccttattg gtcgagcaga gtttcctatt gcttgttcgg tacgtacact atatcggatg
      361 tttaaagata aaacctttga ccctcacgat ttaccaatga aagggaaaag aaaaccaaac
      421 ggacataaag aaaaaagagg aaaacaagct tttcgccgtt cgattcagga tagaacaact
      481 gattatatac agttcaatca tgagtttggt cacctagaag gtgacaccat tgtcggtgga
      541 aaacataaaa gtgcggtgat cacgttagtt gagcgattat ctaaagtgat catcaccttg
      601 aaacccgctg gtaggcaagc tatagatatt gagaatagac tgaatcaatg gtttcaatct
      661 gtaccgaaac acttgtttaa atccatcaca tttgattgtg gaaaagagtt ctcgaattgg
      721 aaaaagatta gtaataccaa tgatatttcg atttatttcg ctgatccagg aacaccttct
      781 caacgaggac taaatgaaca ttcgaatggc ttattacgta aagatggctt atataaagct
      841 atggatttta ataaggtatc cgaaaccttt atccaatcgg tggcatcaaa acgaaatcat
      901 attcctagaa aatcattaca ctatcgcaca ccattggaag tatttttgag t
//
LOCUS       DACCCR010000188          925 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCCR010000188 DACCCR010000000
VERSION     DACCCR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.204"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..276)
                     /locus_tag="IVK93_002797"
     CDS             complement(<1..276)
                     /locus_tag="IVK93_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388000.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYK"
     gene            complement(822..>925)
                     /locus_tag="IVK93_002798"
     CDS             complement(822..>925)
                     /locus_tag="IVK93_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6388001.1"
                     /translation="WTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          286 a          162 c          122 g          355 t
ORIGIN      
        1 tttgtaaaaa tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat
       61 gtcaatattt ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa
      121 ttccctgtaa ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc
      181 acccattcca ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc
      241 agtttgttta tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc
      301 aaaaattttt tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac
      361 tgataattga tactctatat tacatgactt aattagtttt ttagatcccc cctccagata
      421 ggcatatgtt tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc
      481 ataaattaaa aatgactgtc tatttagccc ataatctaat acaacttctt catactcata
      541 tttctttaaa tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt
      601 gttatttatt gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt
      661 agtgaactac tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc
      721 ttgggaagag agcatacttg atagcgtatt actttactaa cagaggtgcc tcttatttac
      781 caagctatcc ccgtagttcc tacggttctt gtttatttga tctaagccaa tcccatagag
      841 tgtagcctta gaccttcggt caggatattg atactagcgt tgatatctcg gtcatgatga
      901 gtatgacaaa tagggcaagt ccatt
//
LOCUS       DACCCR010000189          900 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCCR010000189 DACCCR010000000
VERSION     DACCCR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.205"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            28..>900
                     /locus_tag="IVK93_002799"
     CDS             28..>900
                     /locus_tag="IVK93_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6388002.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          312 a          155 c          169 g          264 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat
//
LOCUS       DACCCR010000190          895 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCCR010000190 DACCCR010000000
VERSION     DACCCR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.206"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..618
                     /locus_tag="IVK93_002800"
     CDS             <1..618
                     /locus_tag="IVK93_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6388003.1"
                     /translation="PFYKEEESLMPYFALAAQRAGAVGIRANSVRDIIEIRKCVSLPM
                     IGIIKRDYPPESPFITATMREVDELVETGVEVIALDCTLRNRHDGLSINSFITKIKEK
                     YPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEESKKQDGPDIDLIKKLVEEGIC
                     VIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS"
     gene            632..>895
                     /locus_tag="IVK93_002801"
     CDS             632..>895
                     /locus_tag="IVK93_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6388004.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAG"
BASE COUNT          313 a          116 c          194 g          272 t
ORIGIN      
        1 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
       61 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
      121 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
      181 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
      241 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
      301 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
      361 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
      421 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
      481 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
      541 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
      601 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
      661 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
      721 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
      781 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
      841 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggt
//
LOCUS       DACCCR010000191          865 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCCR010000191 DACCCR010000000
VERSION     DACCCR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.207"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..208)
                     /locus_tag="IVK93_002802"
     CDS             complement(<1..208)
                     /locus_tag="IVK93_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6388005.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(307..621)
                     /locus_tag="IVK93_002803"
     CDS             complement(307..621)
                     /locus_tag="IVK93_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388006.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
BASE COUNT          284 a          172 c           90 g          319 t
ORIGIN      
        1 aatcagatac ataagcccat gtacccgttc gttgtaaata aagttcttct tgtaacaact
       61 cccattttct cttagaaatc ataatgacgc catcgttcaa tccattttta gaatccctag
      121 taatttctat ttcagaattt gaatgaacta cttcttgcaa cactttacct atattttctt
      181 tgaagtttgt tggagtaata gtatccaaaa tgaatacctc tcttctctat acctctatta
      241 tgaagtgccc ataaaataaa gcaagcgttg aggaataatt ctatcaacac ttgctttatt
      301 aagctttcat tttgtagttt gactaaaacc aataatctta tttctaaact ctcgaaaaac
      361 ttcttgtggt tgataatagt ctggatctat ttcaaatgtt tgtgagaata attcattgaa
      421 tttttctact gaaataatga ttttaccatt cttctctcga ctgttttcta ttatttctgc
      481 ttctaattca aataaatcta gtgacgaact aaaatcataa atcatgtcat attgaataaa
      541 aaaatcaaga aaaagttgct caaactcttc cttatttatc actaatgttt caaacggaac
      601 aactttattt tcctcagcca ttctcccagt tccattttca aaaatcacaa aactgatatt
      661 accttaaaaa ctactaacaa aataaaactt ataaagtaaa tggcaaccca actatgtaac
      721 cattctctct acgtactacg atttggtagt cctgcctttc aatcggtaat tcataatagt
      781 aatttactgt ttcttctttg ccactttcaa tatactctgg attatccaat atcatatcat
      841 actcataaag ttcttcacca tcctt
//
LOCUS       DACCCR010000192          863 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCCR010000192 DACCCR010000000
VERSION     DACCCR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.208"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..861
                     /locus_tag="IVK93_002804"
     CDS             <1..861
                     /locus_tag="IVK93_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP6388007.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DACCCR010000193          820 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCCR010000193 DACCCR010000000
VERSION     DACCCR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.209"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1..797
                     /locus_tag="IVK93_002805"
                     /pseudo
     CDS             1..797
                     /locus_tag="IVK93_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          287 a          153 c          149 g          231 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaaggacga
//
LOCUS       DACCCR010000194          810 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCCR010000194 DACCCR010000000
VERSION     DACCCR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.210"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(16..>810)
                     /locus_tag="IVK93_002806"
     CDS             complement(16..>810)
                     /locus_tag="IVK93_002806"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6388008.1"
                     /translation="RSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQ
                     VLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICE
                     KWAADITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIP
                     EGMILHTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTT
                     SYSDFEEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          221 a          130 c          136 g          323 t
ORIGIN      
        1 ttttagtaga gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt
       61 tagatagtga atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa
      121 tagcgctcga tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt
      181 caatgaggca tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata
      241 agaatgcctt attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct
      301 gcccaagtca gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt
      361 caatgtttga atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt
      421 tttcgtgtgt aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat
      481 gtacgtaatg tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa
      541 gagattctct tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac
      601 aatcgacctt aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat
      661 cccttctttt aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa
      721 aaaaattgtt gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg
      781 tttattctta taaaaataat agacacttct
//
LOCUS       DACCCR010000195          808 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCCR010000195 DACCCR010000000
VERSION     DACCCR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.211"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..693
                     /gene="tnpB"
                     /locus_tag="IVK93_002807"
     CDS             <1..693
                     /gene="tnpB"
                     /locus_tag="IVK93_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP6388009.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          296 a          128 c          148 g          236 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct cccacttc
//
LOCUS       DACCCR010000196          795 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCCR010000196 DACCCR010000000
VERSION     DACCCR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.212"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..434)
                     /locus_tag="IVK93_002808"
     CDS             complement(<1..434)
                     /locus_tag="IVK93_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6388010.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..>795)
                     /locus_tag="IVK93_002809"
     CDS             complement(451..>795)
                     /locus_tag="IVK93_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP6388011.1"
                     /translation="TKYRRQVIDDEISDYAKTTFERISESYHITLVEWNHDKDHVHIM
                     FKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMFWSKSFCLLTTGGAPIDVI
                     KKYIQNQGNNHK"
BASE COUNT          232 a          133 c          126 g          304 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgt
//
LOCUS       DACCCR010000197          788 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCCR010000197 DACCCR010000000
VERSION     DACCCR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.213"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..781
                     /locus_tag="IVK93_002810"
     CDS             <1..781
                     /locus_tag="IVK93_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6388012.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          339 a          106 c          102 g          241 t
ORIGIN      
        1 agatatgcta gattactact attttgaaaa aaatttatca tataattatg taatacattt
       61 aaaaactttt acaggaatga tttttaaatt cgccaaccgt cgatatggat taaaatataa
      121 tcccgtaaat ggggtttctt tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga
      181 aaaagatggt tatttatcaa aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc
      241 acatcaacag tcacgttatt ctttaattac taaatttttg tttttaactg gactacgtta
      301 tggtgaattg atatctttga ctgaatctga ttatgatggt aataaaatta cggtttctgg
      361 tacttatgat tacgagttaa aaattaaaac aacaactaaa aatacaggtt cttatagaac
      421 cattgaatta tcaagtaatg ctaaagaaat catagatcaa ttaattgaag aaaacaaatt
      481 aattaagaat aataaagata aatacatttt catctctaaa aatggtaacc cgatatctat
      541 tcaagcatat aatcaatctt taagagcagt atccaaagaa ttaaacatag ataaaaaagt
      601 ttcttctcac atgttaaggc attcccacat ttctctactt acagaattag gaatcccatt
      661 aaaagccatt atggatcgtg taggacatga agattcaaaa acaacattaa agatttatac
      721 tcatacaact aaaaatatgc aaaatcaact agtagaaaaa ctaggaaaaa tagaaattta
      781 aattgccc
//
LOCUS       DACCCR010000198          751 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCCR010000198 DACCCR010000000
VERSION     DACCCR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.214"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            362..730
                     /locus_tag="IVK93_002811"
     CDS             362..730
                     /locus_tag="IVK93_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6388013.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          243 a          117 c          157 g          234 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt caaaaaatag
       61 cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga gaacaccgtt
      121 taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt ccatataaat
      181 acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa cttatttatt
      241 attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta gattttccca
      301 ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta ggaggaaaca
      361 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
      421 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      481 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      541 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      601 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      661 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      721 aggtaaataa tagaaataaa gactttgttt t
//
LOCUS       DACCCR010000199          729 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCCR010000199 DACCCR010000000
VERSION     DACCCR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.215"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..696
                     /locus_tag="IVK93_002812"
     CDS             <1..696
                     /locus_tag="IVK93_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6388014.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKL
                     RIMIRHGNALIFN"
BASE COUNT          243 a          150 c          128 g          208 t
ORIGIN      
        1 caactgcctt ttttaaaacg ttattttagt catttttcac gagaaattcg tgaagcggtc
       61 gaatggatcg tgattgatat gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca
      121 aaagcacagc tgattattga ccggtttcac atcgtccaac atattggtcg tacctttcgc
      181 aatcatcgaa tcaaagaaac gaaccaacta ttaaaaagca aagaacaaaa acactatcaa
      241 ttaggtaagc aattaaaaag atactggaaa cttcttcaaa aagatgaacg caagcttgat
      301 tatactcgac gcctttggcg acctggcttc aaagctcatt taacagagac cgatattgtg
      361 gatcgcttac tgaaagggag ccctgctttg cgtgtaggct atcagttata tcaagacttt
      421 ctttacgctg taaaggaacg agactacgta tcgtttgaag aactacttac caataatatc
      481 atgcttcccg aaggctatca gacgaccttg agaacgttcc agaaatttct gccacaaatc
      541 aaaaatgctt tacaacaaag ttattcaaat ggcccacttg aatgtctgaa caaccatatc
      601 aaagtgttaa aacgaaatgc atatggcttt cgtagctttt ataatttcaa attgcgcatc
      661 atgattcgtc atggcaacgc attgattttc aactaaaaaa gatcaagagg aactgatcca
      721 cttgatctc
//
LOCUS       DACCCR010000200          729 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCCR010000200 DACCCR010000000
VERSION     DACCCR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.216"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>729
                     /locus_tag="IVK93_002813"
     CDS             <1..>729
                     /locus_tag="IVK93_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6388015.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALV"
BASE COUNT          260 a          139 c          130 g          200 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcc
//
LOCUS       DACCCR010000201          693 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCCR010000201 DACCCR010000000
VERSION     DACCCR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.217"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..447)
                     /locus_tag="IVK93_002814"
     CDS             complement(<1..447)
                     /locus_tag="IVK93_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6388016.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          207 a          119 c           92 g          275 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat att
//
LOCUS       DACCCR010000202          692 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCCR010000202 DACCCR010000000
VERSION     DACCCR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.218"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(176..>692)
                     /gene="mobC"
                     /locus_tag="IVK93_002815"
     CDS             complement(176..>692)
                     /gene="mobC"
                     /locus_tag="IVK93_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP6388017.1"
                     /translation="IAKQRGISRADVCRERLKGLKYEPPLLAEDDRVVLTKQLNQIGN
                     NLNQAVKALNIIAKYFREESEDKKEKSLHFSFDASRYNIFKDELANSIAKKLMISYPD
                     DIFLESNRSAPSFFKLSEVEKEYFEYWIEKQTRPGTKEEYSQKVDRNLIHSIQIIASL
                     KDEVKQLWELL"
BASE COUNT          188 a          139 c           96 g          269 t
ORIGIN      
        1 tgtgctatat ctttagattt attcatcttg gtacgtacta aattcatttc ttgcagtgct
       61 tcctcactat catcactaca atttattcct gagactgcaa cacatttttc tttttttgaa
      121 ggattagctg catattgtaa tagcgaggat aaatttttct tgcgtccttt cgatgttata
      181 gtagctccca aagttgtttc acctcatctt ttaaacttgc aataatctga attgagtgaa
      241 ttaaattccg atcaactttt tgggaatact cttctttcgt acccggtcta gtttgctttt
      301 ctatccaata ttcgaaatat tctttttcta cttctgataa tttaaaaaaa gagggggcac
      361 tccgattgga ctccaaaaaa atgtcatctg gataactaat catcaacttt tttgctatgc
      421 tattcgcaag ttcatcttta aaaatgttgt aacggctagc atcaaaacta aaatgtaggc
      481 tcttttcttt tttatcttca gattcttctc taaaatattt agcaatgata tttaatgcct
      541 ttaccgcttg attcaaattg ttaccaattt gatttaactg tttggttaaa actaccctat
      601 catcttcagc taacaacggc ggttcatatt tgagtccctt taaccgctca cggcaaacgt
      661 ctgctctaga aattcccctt tgttttgcaa tc
//
LOCUS       DACCCR010000203          680 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCCR010000203 DACCCR010000000
VERSION     DACCCR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.219"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..476
                     /locus_tag="IVK93_002816"
     CDS             <1..476
                     /locus_tag="IVK93_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP6388018.1"
                     /translation="RGYNSTKNAYFYGLKIHIIVTKTGFPVTYSITDPSVHDVKVLET
                     LSEEANLPNILGDKGYVSHKIHEKLALKGITVLVPSRKNMDKLGKIDHRLLTRQRKAV
                     ETVFSSLERLGFQNFKSRSIQGLESKFESILLSYSILLSRAQQHFEGTLRYSLGY"
BASE COUNT          236 a          112 c          117 g          215 t
ORIGIN      
        1 gtaggggcta taactcaacg aaaaacgctt atttttatgg tcttaaaatt catattatcg
       61 tcactaaaac aggctttcca gttacttatt caatcacgga tcctagtgtt catgatgtga
      121 aagttttaga gactttatcg gaagaagcaa accttccaaa tatccttgga gataaaggct
      181 atgtcagcca taaaatccat gaaaagcttg cccttaaggg cattacagtc ttagttccgt
      241 cacgaaaaaa catggataaa ctagggaaga tagaccatcg attacttact agacaaagaa
      301 aagcagtaga aactgttttt tcttctctcg aaagactagg ttttcaaaat ttcaaatcac
      361 gttctattca agggctagaa agtaagtttg aaagcatatt attatcgtac agtattcttt
      421 taagccgagc acagcaacat tttgaaggga ctttgagata ttctttaggg tattaaaatt
      481 aactagcacc acgggtatat ttttattata cacagcttgt cgcttggata gaactttagt
      541 ataattaaac gaaaaagagg taaatgaaat ggaaatgtta gtcaagtcca gactcctgtg
      601 taaaatgcta tacaatgttt ttaccatttc tacttatcaa aattgatgta ttttcttgaa
      661 gaataaatcc attcatcatg
//
LOCUS       DACCCR010000204          679 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCCR010000204 DACCCR010000000
VERSION     DACCCR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.220"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          240 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatt
//
LOCUS       DACCCR010000205          652 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCCR010000205 DACCCR010000000
VERSION     DACCCR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.221"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            99..>652
                     /locus_tag="IVK93_002817"
     CDS             99..>652
                     /locus_tag="IVK93_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HAP6388019.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIM"
BASE COUNT          206 a          133 c          118 g          195 t
ORIGIN      
        1 gacaccagca tcttttttat tttccaaaac atatagaaaa gacaccttga aatcctttag
       61 aattaatttg gcgaaccaac ccaaaaggag gaatcaagat gtcttacact caccttatca
      121 aagaaacatt agatattcta gacttatcag ttacttttaa cgaaaattgt ctcacaaaag
      181 aaaaatacaa agggcaaatc tgtcatattt atcgtggaaa cttgatttat acagcccaag
      241 aatgcatcca ctgtaaacac cagattgctt cagatattgt gcgttggggt acaacaaccg
      301 tccggttgtt aatgaacgat gtttctgaat accgtaccta cctcgaattg aaaaagcaac
      361 gcttcaaatg caaagcttgt cagcggacct ttgtcgcaga tacttctgtt gcgaaaaaac
      421 attgtttcat cagtgagaaa gtccgctggt ctgtcgtgac tcgtctgaaa aaaaatacct
      481 caatgacaga gatcgccgct cagaaaaatc tatcggtctc ttctgtttac cgtattatga
      541 agcgatttta tcgtccgttg aatccttttc ggacaccgtt acctaaggta ctttgttttg
      601 atgaattcaa atcagttcgg ggtgtttcag gtgctatgag tttcatcatg at
//
LOCUS       DACCCR010000206          605 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCCR010000206 DACCCR010000000
VERSION     DACCCR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.222"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>605
                     /locus_tag="IVK93_002818"
     CDS             <1..>605
                     /locus_tag="IVK93_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP6388020.1"
                     /translation="KTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV
                     IIGRKERSVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRP
                     DNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQW
                     FSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          206 a          110 c          113 g          176 t
ORIGIN      
        1 aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa
       61 gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac tcttggttgg
      121 acgcctgacg tcattatcgg gcgaaaagaa aggtctgttt catgcggtat gagaacactt
      181 tatcgtttat tttctaaagg aatatttgat attgacacac taccgatgaa aggtaaaaga
      241 aaacccaatg gccatcagga aaaacgggga aaacaacagt atcagcgctc aatccatgat
      301 cgacctgata attatcctga tttcaattct gagtttggtc accttgaagg tgatacgatc
      361 gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc taaagtcatt
      421 atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct taatcaatgg
      481 ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg aaaagaattt
      541 tctaactgga aagccattag taaccaacat gatattgata tatattttgc ggaccctgga
      601 acacc
//
LOCUS       DACCCR010000207          592 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCCR010000207 DACCCR010000000
VERSION     DACCCR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.223"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..579
                     /locus_tag="IVK93_002819"
     CDS             <1..579
                     /locus_tag="IVK93_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6388021.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          203 a          105 c          122 g          162 t
ORIGIN      
        1 ggaatcaaat cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc
       61 ggttatcttt ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt
      121 tttacggatg ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca
      181 gtaattgatc caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac
      241 gcccacgttg atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac
      301 attgctaatt ataaatacga gtatattttc attatggact acaatactgg aaaagaacta
      361 gctagagtaa gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta
      421 ggaaatgttg gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac
      481 gtcatgatgc gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt
      541 catgacaagc gttggtattt aaatattcca caacgataaa aaattccccc ct
//
LOCUS       DACCCR010000208          579 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCCR010000208 DACCCR010000000
VERSION     DACCCR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.224"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..208
                     /locus_tag="IVK93_002820"
     CDS             <1..208
                     /locus_tag="IVK93_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP6388022.1"
                     /translation="TNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVIPENWRK
                     SLQNDQLLKTISQRFCDTVLSQRI"
     gene            277..543
                     /locus_tag="IVK93_002821"
     CDS             277..543
                     /locus_tag="IVK93_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6388023.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
BASE COUNT          203 a           94 c          123 g          159 t
ORIGIN      
        1 tacgaatagt tatcaagcag ccatattgac tgcggtcaat ttaggagggg atactgatac
       61 tactgctgcc gtaactggtg ctcttgcggg tattctttac gggggtgaag tgatccctga
      121 aaattggaga aaatcgcttc aaaatgacca gcttcttaaa acgatcagtc agagattttg
      181 cgataccgtg ctatctcaaa gaatttgaat aatggatatg atgcaaatct tgtttgtact
      241 aacgttgtat tgacaaaaag gattttattt agtacaatgt tagtacaaat gatgaacgga
      301 ggtagaatca tgtctcaatc aaccattaca tttaggattc ccgacgatga aaaagaactg
      361 gtattagaat atgcaaaagc acacaattct tcattgaccg aactttatcg aaatgctgta
      421 ctggataaaa tcgaagatga aatcgatcta cggactttac ggagtgcaat tgaagtttcc
      481 aaagagaaaa aagagagtgg tatttctcaa gaggaaatgg agaaactgct aaatgaatta
      541 taaagtcatc tacatgccgc aagcccagaa gcaattaaa
//
LOCUS       DACCCR010000209          565 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCCR010000209 DACCCR010000000
VERSION     DACCCR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.225"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(208..453)
                     /locus_tag="IVK93_002822"
     CDS             complement(208..453)
                     /locus_tag="IVK93_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388024.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT          172 a           97 c           70 g          226 t
ORIGIN      
        1 aaattataac agaaataaat atttatttca aaaatatcct acaaaagtct aaaatccgta
       61 attatttctt atatgcaaca tttacaaata aagtcgctct gagagctcgt atttttcgat
      121 ttaagacgtt ttatttagtt tttggtgtat ttcattggtc caagctaaaa aactcttaga
      181 agcgatttta gagccattta aacccactta attatattct ccatgaaatt cgctccatgc
      241 gtcaaaagct gcgtctgaaa ttccgccgtc ttcgttcact agttgcatta atttttccaa
      301 cgatcgtttt ctttcgggtg tcatattttt atattccaac aatttttcgt ctgttaaatc
      361 gaacttgatt gcttgaattg cttgctctaa tttttctaag ttttgaaaat attcgtaagg
      421 aaatttttcc attttttctt tctccagtct cacaattttt ctagaaataa aatcacttct
      481 ttgccgtcta tactctcaaa aaaaatgtcg tttgaaaaac taaataaatt tttgatcgtg
      541 catttttaaa tttggttatc gatta
//
LOCUS       DACCCR010000210          555 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCCR010000210 DACCCR010000000
VERSION     DACCCR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.226"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>555
                     /gene="tnpB"
                     /locus_tag="IVK93_002823"
     CDS             <1..>555
                     /gene="tnpB"
                     /locus_tag="IVK93_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP6388025.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCG"
BASE COUNT          204 a           87 c           97 g          167 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt gtgga
//
LOCUS       DACCCR010000211          553 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCCR010000211 DACCCR010000000
VERSION     DACCCR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.227"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          172 a           87 c           58 g          236 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatagca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt ctt
//
LOCUS       DACCCR010000212          552 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCCR010000212 DACCCR010000000
VERSION     DACCCR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.228"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            68..>552
                     /locus_tag="IVK93_002824"
     CDS             68..>552
                     /locus_tag="IVK93_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP6388026.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     F"
BASE COUNT          207 a          100 c           90 g          155 t
ORIGIN      
        1 aaaaaaggaa gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga
       61 cttccctatg aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt
      121 aaatgaatta ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt
      181 aacggctttt ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag
      241 aaacggttct tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc
      301 tagagatcgt aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga
      361 tacattggaa accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc
      421 tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac
      481 taaagttctg actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt
      541 cgctattttt at
//
LOCUS       DACCCR010000213          548 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCCR010000213 DACCCR010000000
VERSION     DACCCR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.229"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>548)
                     /locus_tag="IVK93_002825"
     CDS             complement(<1..>548)
                     /locus_tag="IVK93_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6388027.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DACCCR010000214          528 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCCR010000214 DACCCR010000000
VERSION     DACCCR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.230"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(5..90)
                     /locus_tag="IVK93_002826"
     tRNA            complement(5..90)
                     /locus_tag="IVK93_002826"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IVK93_002827"
     tRNA            complement(107..178)
                     /locus_tag="IVK93_002827"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IVK93_002828"
     tRNA            complement(190..262)
                     /locus_tag="IVK93_002828"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IVK93_002829"
     tRNA            complement(280..361)
                     /locus_tag="IVK93_002829"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IVK93_002830"
     tRNA            complement(369..441)
                     /locus_tag="IVK93_002830"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IVK93_002831"
     tRNA            complement(445..517)
                     /locus_tag="IVK93_002831"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACCCR010000215          523 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCCR010000215 DACCCR010000000
VERSION     DACCCR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.231"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            152..412
                     /locus_tag="IVK93_002832"
     CDS             152..412
                     /locus_tag="IVK93_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388028.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          179 a           61 c           72 g          211 t
ORIGIN      
        1 aagtcctctt ttctgtgaat tggttgtcgt taactttatt ctacagaagc gacttccttt
       61 tttgtatgga ttttttcatt tacacaaaat attttacact ctcaattatt ttaatataag
      121 tacttcagat ttaataaagg agaaatatct aatgaaaaaa attgatttaa taaatatgat
      181 aggtatgtta atagggatat tagtaaatat agtaattttc acagattggc tcggggtgtt
      241 attttctaat cttattccca tacttattat aggcatttgt ggaattatac tttcaatatt
      301 agaacttttt gaaagcagaa atacaatgaa tagaatattt gcttgtatta tattaattgt
      361 taatctattg cctatggtct attttacttt tttatatttt gcactgggat gaaataaatt
      421 aaccaaaagt ttctttttct tataatgtgt atcaatatta aaactgaaaa agatatggat
      481 aataggatca aagccctcat tggaacactc aatatattat cag
//
LOCUS       DACCCR010000216          514 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCCR010000216 DACCCR010000000
VERSION     DACCCR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.232"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..132)
                     /locus_tag="IVK93_002833"
     CDS             complement(<1..132)
                     /locus_tag="IVK93_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6388029.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACCCR010000217          509 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCCR010000217 DACCCR010000000
VERSION     DACCCR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.233"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          194 a           60 c           67 g          188 t
ORIGIN      
        1 tatgttaatt tatactaaat taaataaagt actaccttta ataggtagta ctttattatc
       61 aagccattat tccttcttct actagttctt tttgtcacat aattttgcaa tcaataaatg
      121 ttgtgcaata agtataagaa ttccattttc taaatttgaa aggtgatcca taatttcatg
      181 ttcaagtagt ttattaattg tccacttgtc gcttcatcta aaaattttat aggttttagg
      241 aatgctattc gcttatttta ggaagatttt aactgtttgc tatagatata tatcatacta
      301 gtatcgtaat aaaagtactc taaaagaaga attttcaaat acgcaaaatt aagtacgaaa
      361 gagttgaaac aaatttggag aaaatgagca tagaatgtct gatgagttaa atagacctaa
      421 aaattatcta aaagtggagc aaaattagga attacaaaca aaacactaaa ttagtttata
      481 taattttccg taatatttaa tatatatga
//
LOCUS       DACCCR010000218          504 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCCR010000218 DACCCR010000000
VERSION     DACCCR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.234"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          226 a           70 c           81 g          127 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actc
//
LOCUS       DACCCR010000219          500 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCCR010000219 DACCCR010000000
VERSION     DACCCR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.235"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>500
                     /locus_tag="IVK93_002834"
     CDS             <1..>500
                     /locus_tag="IVK93_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388030.1"
                     /translation="SLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFK
                     IEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEI
                     VILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDS
                     DPLLQY"
BASE COUNT          179 a           82 c          100 g          139 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta
//
LOCUS       DACCCR010000220          487 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCCR010000220 DACCCR010000000
VERSION     DACCCR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.236"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>487)
                     /locus_tag="IVK93_002835"
     CDS             complement(<1..>487)
                     /locus_tag="IVK93_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6388031.1"
                     /translation="GFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLG
                     FFGGSRFIPIVSSLAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRL
                     TGAVGLHHTIYPLFWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFA
                     G"
BASE COUNT          184 a           97 c           80 g          126 t
ORIGIN      
        1 cttcctgcaa aaaatctagt accataagta aacagaccag tgtggtttgg atcagctagt
       61 tgggcaaaga aaatattttg ggctccagca attgttgatc cagctacagt ttcggtacca
      121 cctaaagatg tataccagaa caaagggtaa atggtgtggt gtagtccaac agcacctgtt
      181 aaacgcaata aaaaaccata taaaaaagtt cctaaactac ccatttgggc aatttgttca
      241 ccagcaacta ctaaaccatt ttgaattggt ggccaaatta aataaaagaa agaaccaata
      301 atgattgcag ctaaactaga aacaatcgga ataaatcttg atcctccaaa aaaacctaag
      361 aattgtggta attcaatttt tctataacga ttgtgtaaaa atgcaacagt accaccaaca
      421 acaattgatc ccactacacc tgtatctagt gttgcatctt ttgctgaaaa aagttcaaga
      481 aacccac
//
LOCUS       DACCCR010000221          487 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCCR010000221 DACCCR010000000
VERSION     DACCCR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.237"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>487)
                     /locus_tag="IVK93_002836"
     CDS             complement(<1..>487)
                     /locus_tag="IVK93_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP6388032.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACCCR010000222          484 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCCR010000222 DACCCR010000000
VERSION     DACCCR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.238"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            43..249
                     /locus_tag="IVK93_002837"
     CDS             43..249
                     /locus_tag="IVK93_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388033.1"
                     /translation="MVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQ
                     ISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          211 a           62 c           50 g          161 t
ORIGIN      
        1 ggtaaaaaaa taaaaaaatg tgacaagtgt tttgaatcag ctgtggtaaa gtttcctcaa
       61 tttaaactaa atccagctgc ctcgatacaa tttcaaggac atgtatttga tgttttagct
      121 aaagataaca taaaaaaata caaaaaaggt aatatattcc atattgatat acaaatatct
      181 tctaatttag aagatataaa aaaagccttt aaaattaaaa caattggcac tgctcctaat
      241 tttaaataac aaaaaaatta ctagataatc taaaaacagg atggcattat gagaacaata
      301 actaaacttc ctatatcaca tattttgtaa ttagtgaaaa gcaaattgac tactatcata
      361 agttaatgac tctcatctta atatttaaaa tatattcata tttttctatt ttatctaaat
      421 gcgattttca tatctaataa tagaaaaata aacaattaac aataaaaaag tcttcattta
      481 ttgt
//
LOCUS       DACCCR010000223          462 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCCR010000223 DACCCR010000000
VERSION     DACCCR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.239"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..430)
                     /locus_tag="IVK93_002838"
     CDS             complement(<1..430)
                     /locus_tag="IVK93_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6388034.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACCCR010000224          444 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCCR010000224 DACCCR010000000
VERSION     DACCCR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.240"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..379
                     /locus_tag="IVK93_002839"
     CDS             <1..379
                     /locus_tag="IVK93_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6388035.1"
                     /translation="HRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTE
                     GFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIA
                     FGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          141 a           93 c           80 g          130 t
ORIGIN      
        1 tcatcgactg tttaaacgac caattagtca aacagaaatc gttgatgagc tgttaagcta
       61 caacgaagaa ttgcgaactg cgtatcattt ttgtcaatta ttacgctatt atttcgttaa
      121 acgaggtacg gaaggctttc aagaaaccct taaaacaatt tcatctacgt tacctcagtc
      181 gtttaaaaag aaattcacga tcttccatcg ctaccaatcg ggtatttcaa atgcctttaa
      241 ggtgtcccac tcaaacggcg tcactgaggg gctgaataac aagatcaagc tgattaaacg
      301 aattgcgttt ggttatcgca acttttataa ctttcgggca cggatctatt tacaacaagg
      361 tctgatcttt gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat atcggactaa
      421 gttttattca aatttgtctt cacc
//
LOCUS       DACCCR010000225          435 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCCR010000225 DACCCR010000000
VERSION     DACCCR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.241"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            201..>435
                     /locus_tag="IVK93_002840"
     CDS             201..>435
                     /locus_tag="IVK93_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6388036.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITS"
BASE COUNT          159 a           62 c           77 g          137 t
ORIGIN      
        1 tatttgagaa taacatttta tttaatcttt tcatatatca attgtatgaa aaattattag
       61 aattttaaaa aatgataaaa attcaaaaag aaaaaatagt agttatttac ttgtcatgtt
      121 actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga tttcattcta
      181 aaataaaggg agaaatgtct atgctatacg gtgcaattga agcaggcggc acaaaatttg
      241 tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt ccgacaacga
      301 cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa gcagatttag
      361 cagggattgg gattggctca tttgggccta tcgatatcca tcgcgattcc gccacttatg
      421 ggtacattac ctcga
//
LOCUS       DACCCR010000226          407 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCCR010000226 DACCCR010000000
VERSION     DACCCR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.242"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..384
                     /locus_tag="IVK93_002841"
     CDS             <1..384
                     /locus_tag="IVK93_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6388037.1"
                     /translation="RKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIE
                     NQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTY
                     NQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          157 a           73 c           64 g          113 t
ORIGIN      
        1 cgaaaagaaa tctgtgatga ctttaaggct gtttatcaag ctaactcaaa agaagaagcg
       61 aataccttct tatccggcat gattgagaaa tggaagaaaa actatcctaa agtgacgcag
      121 tcactcatag aaaaccaaga cttattaact ttttatgatt ttccacctag cattcgtaga
      181 accatttact caaccaatct aatcgagtct ttcaataagc aaattaaaag atacagccgt
      241 agaaaagagc agtttcaaaa tgaagaatca ctagaacgct ttctagtcag catttttgat
      301 acatacaatc aaaaatttct aaacagaagc cataaaggtt ttcaacaggt aaccgataca
      361 ttagtttcaa tgtttactga gtaactaatt attttgcagg aggacaa
//
LOCUS       DACCCR010000227          388 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCCR010000227 DACCCR010000000
VERSION     DACCCR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.243"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1..388
                     /locus_tag="IVK93_002842"
                     /pseudo
     CDS             1..388
                     /locus_tag="IVK93_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          153 a           68 c           75 g           92 t
ORIGIN      
        1 cgtcaatctt tacttagctg gaaagccacg tgtagaaatc attcgagaat atgaactaac
       61 ggcttcagca tttgacaaat gggtaaagca atctaaaacg agtggttcat tcaaagaaaa
      121 agataatctt acgcctgaac aaaaagaatt gttagaacta cgtaaaagaa accagcaatt
      181 agaaatggaa aatgatattt taaagcaagc agcgctgata ttcggacgaa gagacaagta
      241 atcgatgcga ataagcatct ttaccctata tcagcgatgt gcagaatatt aggtctatca
      301 cgtcagtcct attattatca atcaaaacca aagaaagacg aatcagaact tgaagaagta
      361 gtcgctgaag aatttatccg cagccgaa
//
LOCUS       DACCCR010000228          381 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCCR010000228 DACCCR010000000
VERSION     DACCCR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.244"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          164 a           50 c           38 g          129 t
ORIGIN      
        1 aaacaaaaat aaataaagtc ggatttttct gataattcaa cgcaaaaaaa cgaacaattt
       61 ttcaataaat cagtcttcta aaatagataa cctaaaaaaa acgaacaaac aaataaaatg
      121 actgtccttc attcactaaa catattttta ttttttatat tgttaaaaat tcacaattat
      181 tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt tttaacggca
      241 tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt cataatattt
      301 cccatattgt gcaaaaaaat acaaggtgga ggctctacta tgattgaaga atatattgag
      361 aaaaatattt taagacagct g
//
LOCUS       DACCCR010000229          346 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCCR010000229 DACCCR010000000
VERSION     DACCCR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.245"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..168
                     /locus_tag="IVK93_002843"
     CDS             <1..168
                     /locus_tag="IVK93_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6388038.1"
                     /translation="PIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVS
                     TEIKVLMGITA"
BASE COUNT          131 a           57 c           66 g           92 t
ORIGIN      
        1 cctattaaac gacgaaataa attttatcaa agtctccgta cagcctcttc cacgattaag
       61 ggcatggaga ccattcgagg aatatataaa aagaaccgaa gaaatggaac gctcttcggc
      121 ttttcggtgt ctactgaaat caaggtatta atgggaataa cagcctaaga tatttggagt
      181 tcagagaggg cgcgtttgat tttcaaactt cgcaacagaa ccaaagaagg tatcaagagt
      241 cacagtgaat ttattccgaa aagtgaaaag agcaaatata aaatttaata aaaatataga
      301 actaaatagg aaaacgtaga cgtaattttc tacgttttcc ttttca
//
LOCUS       DACCCR010000230          339 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCCR010000230 DACCCR010000000
VERSION     DACCCR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.246"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..202
                     /gene="tnpB"
                     /locus_tag="IVK93_002844"
     CDS             <1..202
                     /gene="tnpB"
                     /locus_tag="IVK93_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP6388039.1"
                     /translation="QDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVR
                     WLLEGLSIDQPRAILPGKKGVF"
BASE COUNT          102 a           57 c           78 g          102 t
ORIGIN      
        1 tcaagaccgt ttcaaacttc tctattggga cggtgatggt tttcttctct gttataagcg
       61 gatagaaaac ggcaagttaa aatggcctcg gacgaaagac gaagttcgaa ccttaacgaa
      121 ccaacaggtt cgatggttgt tagaaggatt aagtatcgat caacctcgag ctattttgcc
      181 aggaaaaaaa ggagtttttt aagctggggg gttaacacaa aaaatgtgtt gaacccctat
      241 ttcttgtgcg tttcttgtgg tattctgtaa caaaatcagt tcgattcaag gtggtgaaaa
      301 attttggaaa ctacagatac gttgcttcag ttgcttcaa
//
LOCUS       DACCCR010000231          333 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCCR010000231 DACCCR010000000
VERSION     DACCCR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.247"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>333
                     /locus_tag="IVK93_002845"
     CDS             <1..>333
                     /locus_tag="IVK93_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6388040.1"
                     /translation="AGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASY
                     IAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPA
                     LEDNAGTLG"
BASE COUNT           98 a           72 c           82 g           81 t
ORIGIN      
        1 gctggcagct gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc
       61 gaaaaacgct tagggaaaaa aggacaagag gttgcagaag atgatcctta ttgggaaatc
      121 gaagcctcct atattgctca atgtgcctac aatacaacct tgatgttttc tccagatgtg
      181 atcatttttg gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca
      241 tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt taagcaaata tatcgtgacc
      301 ccagcattag aagacaacgc tggaacgctc ggt
//
LOCUS       DACCCR010000232          332 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCCR010000232 DACCCR010000000
VERSION     DACCCR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.248"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>332
                     /locus_tag="IVK93_002846"
     CDS             <1..>332
                     /locus_tag="IVK93_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388041.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DACCCR010000233          321 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCCR010000233 DACCCR010000000
VERSION     DACCCR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.249"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            94..>321
                     /locus_tag="IVK93_002847"
     CDS             94..>321
                     /locus_tag="IVK93_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6388042.1"
                     /translation="MFGLLFEKMLQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKT
                     SFGKVNIDVPRDREASFEPELIPNRKRDVSDI"
BASE COUNT          103 a           58 c           70 g           90 t
ORIGIN      
        1 aaaaacgcca aacattcccg attctattct ataggtcggg ttatttgacg tacaggtact
       61 gttctatggg gcgcttacga tgttttaaaa gatgtgtttg gtctcttatt tgaaaaaatg
      121 cttcaaggag aattgaataa ccatttaggg tatgacgccc attctaaaga gccaaagaaa
      181 catgacaatc gacgaaacgg ttatggcaat aagacgctta aaacgagctt tggtaaagta
      241 aacattgatg ttcccagaga ccgggaggct tcttttgaac ctgagttgat tcctaataga
      301 aaacgagatg tctccgacat c
//
LOCUS       DACCCR010000234          321 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCCR010000234 DACCCR010000000
VERSION     DACCCR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.250"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>321)
                     /locus_tag="IVK93_002848"
     CDS             complement(<1..>321)
                     /locus_tag="IVK93_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6388043.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACCCR010000235          318 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCCR010000235 DACCCR010000000
VERSION     DACCCR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.251"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>318
                     /locus_tag="IVK93_002849"
     CDS             <1..>318
                     /locus_tag="IVK93_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP6388044.1"
                     /translation="KKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYG
                     FYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEG
                     TLFG"
BASE COUNT          115 a           66 c           62 g           75 t
ORIGIN      
        1 taaaaaacgg gacacacaag cagcctatgc ttttcttaag cggttagtga agcagtttga
       61 tgaaccgaag gttgtagtca cagataaagc cccctctatt acaagtgcct ttaagaaact
      121 aaaagaatac ggcttttatc aagggacaga acatcgtacc attaaatacc tgaataattt
      181 gattgaacaa gaccatcgtc cagtaaagag acgcaataaa ttctatcgaa gtttacgcac
      241 tgcctctacc acgattaaag gcatggaagc cattcgagga ttatataaga aaacccgaaa
      301 agaaggcact ctcttcgg
//
LOCUS       DACCCR010000236          309 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCCR010000236 DACCCR010000000
VERSION     DACCCR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.252"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            51..>309
                     /locus_tag="IVK93_002850"
     CDS             51..>309
                     /locus_tag="IVK93_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6388045.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          120 a           41 c           61 g           87 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggc
//
LOCUS       DACCCR010000237          294 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCCR010000237 DACCCR010000000
VERSION     DACCCR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.253"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>294
                     /locus_tag="IVK93_002851"
     CDS             <1..>294
                     /locus_tag="IVK93_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388046.1"
                     /translation="FKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKIV
                     TKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRP"
BASE COUNT          113 a           43 c           51 g           87 t
ORIGIN      
        1 gtttaaagtt tacttcccgt ttcagtggct cgttaaagga attcttttag tgctactagg
       61 attgattctt tttggaagtt atctaacaat taaagccaga cattttccag aagaaaagcc
      121 aataaaaatt gtaacaaaag atttaaaaat aggtgaaact tatcaggcag atggggttga
      181 actaacattt aaaaatattg aagtgaccga aaaaagaaat gaatttgcac aacatgatcg
      241 tgtattacaa gtgacctata cacttataaa tgatacagaa aatccgatac gtcc
//
LOCUS       DACCCR010000238          287 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCCR010000238 DACCCR010000000
VERSION     DACCCR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.254"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          102 a           31 c           45 g          109 t
ORIGIN      
        1 aggactacct gtattagtcc tcttttttga ccagtctatt ttacattcta atatattcat
       61 atgagctaat tttatgatta aatggtaagt ttttccttgt ttgttttagt ttaaactagt
      121 gtgtaattca aaaatctata tggaaggatc tgtggggtaa atcattatac aaaagtaaaa
      181 ttttacttat taacaggctg tagaaatata aaaaagaact aaaatttaac taaattatgt
      241 gctattctta aggaaagcca agtgtatgag aaagtttctg agaatat
//
LOCUS       DACCCR010000239          280 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCCR010000239 DACCCR010000000
VERSION     DACCCR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.255"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            166..>280
                     /locus_tag="IVK93_002852"
     CDS             166..>280
                     /locus_tag="IVK93_002852"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6388047.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLE"
BASE COUNT           95 a           48 c           56 g           81 t
ORIGIN      
        1 ttgttcctgt ccaattagga aacgaaaaga gtgcaccaga aaaaggtaaa cccgcggctg
       61 ccaagttagc gatgcgcatc aaaagagaga atggttctga agttttgatc tataacggta
      121 ttaacaatta tattcttcaa gctattttga aagaactgtt gtccaatgct ttatgattta
      181 cttcaaacca aacatattta tattgtgtgc ggaaaaacag atttgcgaaa aggaattgat
      241 ggtcttgcca gtcttattca acaagagtat caactcgagc
//
LOCUS       DACCCR010000240          275 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCCR010000240 DACCCR010000000
VERSION     DACCCR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.256"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            135..>275
                     /locus_tag="IVK93_002853"
     CDS             135..>275
                     /locus_tag="IVK93_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HAP6388048.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAK"
BASE COUNT           91 a           56 c           46 g           82 t
ORIGIN      
        1 attaaaacca tgtgaaactg cctcacggca gcttacaatt actttttcat cttcacccgt
       61 taagtgatga aaaaagaacc cctttctgct atggttaaag tgactaaacc aaccaaaaga
      121 aaggagttct tttcatgact agcttacaca aaaaccaagt aaaattcaat tcaaatatca
      181 ctatttctca tacaggtggt caattatcga gtgattcggg tctcgtccta gtgaaagagc
      241 tgatgaacat ctttggtttt tctgaactgg ctaag
//
LOCUS       DACCCR010000241          268 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCCR010000241 DACCCR010000000
VERSION     DACCCR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.257"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            87..>268
                     /locus_tag="IVK93_002854"
     CDS             87..>268
                     /locus_tag="IVK93_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6388049.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           55 c           43 g           86 t
ORIGIN      
        1 tttacctatg aaatattttc acgtgttaaa gtattcctcg ttgaacacaa caaataaaac
       61 tttttaacga ataaaggaga tttttcatga catcacttaa aactttactt tttggaacaa
      121 ctttggctgc cggcgctgca ttcttcatgg gaacgactgc tcatgcggac gaagcttata
      181 ccgttcaatc aggtgatact ctatcaacga tttctcaaaa atacgttggt gataactcct
      241 tgatcaatgc cattgcagaa tcaaattc
//
LOCUS       DACCCR010000242          257 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCCR010000242 DACCCR010000000
VERSION     DACCCR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.258"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>257
                     /locus_tag="IVK93_002855"
     CDS             <1..>257
                     /locus_tag="IVK93_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP6388050.1"
                     /translation="KAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELED
                     EINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKG"
BASE COUNT          103 a           42 c           58 g           54 t
ORIGIN      
        1 aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat caaagctaac
       61 gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc ctatcaggat
      121 gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc ttttactttg
      181 gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga tccggaaagc
      241 ggctactacg tcaaagg
//
LOCUS       DACCCR010000243          256 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCCR010000243 DACCCR010000000
VERSION     DACCCR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.259"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            13..>256
                     /locus_tag="IVK93_002856"
     CDS             13..>256
                     /locus_tag="IVK93_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6388051.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDR"
BASE COUNT           76 a           59 c           56 g           65 t
ORIGIN      
        1 gaggacttcc atatgaacga ttttactaca gaaattctaa agactctagc gaacaaaggc
       61 gatttgaatg aattattccg tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg
      121 gagttaacgg ctttcctcga ttacgaaaag tacgatcgca ttggttttaa cacggggaat
      181 tctcgtaacg gctcctatga ccgtacggtc aagaccgagt acggggaact tcatctccag
      241 attccgcgcg accgca
//
LOCUS       DACCCR010000244          244 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCCR010000244 DACCCR010000000
VERSION     DACCCR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.260"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>244
                     /locus_tag="IVK93_002857"
     CDS             <1..>244
                     /locus_tag="IVK93_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP6388052.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFKLPDIGEG"
BASE COUNT           77 a           50 c           65 g           52 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaa
//
LOCUS       DACCCR010000245          241 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCCR010000245 DACCCR010000000
VERSION     DACCCR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.denovo.261"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..220)
                     /locus_tag="IVK93_002858"
     CDS             complement(<1..220)
                     /locus_tag="IVK93_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6388053.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DACCCR010000246        34862 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.025, whole genome shotgun sequence.
ACCESSION   DACCCR010000246 DACCCR010000000
VERSION     DACCCR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.025"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            113..586
                     /locus_tag="IVK93_002859"
     CDS             113..586
                     /locus_tag="IVK93_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6388054.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            682..1569
                     /gene="xerD"
                     /locus_tag="IVK93_002860"
     CDS             682..1569
                     /gene="xerD"
                     /locus_tag="IVK93_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP6388055.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            1588..1959
                     /locus_tag="IVK93_002861"
     CDS             1588..1959
                     /locus_tag="IVK93_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6388056.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            1929..2726
                     /locus_tag="IVK93_002862"
     CDS             1929..2726
                     /locus_tag="IVK93_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP6388057.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            2710..3279
                     /gene="scpB"
                     /locus_tag="IVK93_002863"
     CDS             2710..3279
                     /gene="scpB"
                     /locus_tag="IVK93_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP6388058.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            3285..4001
                     /locus_tag="IVK93_002864"
     CDS             3285..4001
                     /locus_tag="IVK93_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6388059.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      4083..4208
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4331..4942
                     /locus_tag="IVK93_002865"
     CDS             4331..4942
                     /locus_tag="IVK93_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6388060.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            5320..6843
                     /locus_tag="IVK93_002866"
     CDS             5320..6843
                     /locus_tag="IVK93_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP6388061.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            7080..8420
                     /locus_tag="IVK93_002867"
     CDS             7080..8420
                     /locus_tag="IVK93_002867"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP6388062.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            8573..9310
                     /locus_tag="IVK93_002868"
     CDS             8573..9310
                     /locus_tag="IVK93_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP6388063.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(9475..9696)
                     /locus_tag="IVK93_002869"
     CDS             complement(9475..9696)
                     /locus_tag="IVK93_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP6388064.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            9727..10758
                     /locus_tag="IVK93_002870"
     CDS             9727..10758
                     /locus_tag="IVK93_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388065.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10745..12148
                     /locus_tag="IVK93_002871"
     CDS             10745..12148
                     /locus_tag="IVK93_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP6388066.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            12214..12849
                     /locus_tag="IVK93_002872"
     CDS             12214..12849
                     /locus_tag="IVK93_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6388067.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            12898..13578
                     /locus_tag="IVK93_002873"
     CDS             12898..13578
                     /locus_tag="IVK93_002873"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP6388068.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            13713..14942
                     /gene="rpsA"
                     /locus_tag="IVK93_002874"
     CDS             13713..14942
                     /gene="rpsA"
                     /locus_tag="IVK93_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP6388069.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            15124..16434
                     /locus_tag="IVK93_002875"
     CDS             15124..16434
                     /locus_tag="IVK93_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP6388070.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            16677..16952
                     /locus_tag="IVK93_002876"
     CDS             16677..16952
                     /locus_tag="IVK93_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP6388071.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            17187..18233
                     /locus_tag="IVK93_002877"
     CDS             17187..18233
                     /locus_tag="IVK93_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388072.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            18235..19494
                     /locus_tag="IVK93_002878"
     CDS             18235..19494
                     /locus_tag="IVK93_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6388073.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            19500..20081
                     /locus_tag="IVK93_002879"
     CDS             19500..20081
                     /locus_tag="IVK93_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP6388074.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(20163..21038)
                     /locus_tag="IVK93_002880"
     CDS             complement(20163..21038)
                     /locus_tag="IVK93_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6388075.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            21186..21518
                     /locus_tag="IVK93_002881"
     CDS             21186..21518
                     /locus_tag="IVK93_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6388076.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            21630..22838
                     /locus_tag="IVK93_002882"
     CDS             21630..22838
                     /locus_tag="IVK93_002882"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP6388077.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            22992..23447
                     /locus_tag="IVK93_002883"
     CDS             22992..23447
                     /locus_tag="IVK93_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388078.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            23809..24084
                     /locus_tag="IVK93_002884"
     CDS             23809..24084
                     /locus_tag="IVK93_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388079.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            24097..26175
                     /locus_tag="IVK93_002885"
     CDS             24097..26175
                     /locus_tag="IVK93_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6388080.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            26335..28221
                     /locus_tag="IVK93_002886"
     CDS             26335..28221
                     /locus_tag="IVK93_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6388081.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            28233..29180
                     /locus_tag="IVK93_002887"
     CDS             28233..29180
                     /locus_tag="IVK93_002887"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6388082.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            29200..29727
                     /locus_tag="IVK93_002888"
     CDS             29200..29727
                     /locus_tag="IVK93_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6388083.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(29790..30443)
                     /locus_tag="IVK93_002889"
     CDS             complement(29790..30443)
                     /locus_tag="IVK93_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6388084.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            30575..31417
                     /locus_tag="IVK93_002890"
     CDS             30575..31417
                     /locus_tag="IVK93_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6388085.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            31575..32471
                     /locus_tag="IVK93_002891"
     CDS             31575..32471
                     /locus_tag="IVK93_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6388086.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            32474..33187
                     /locus_tag="IVK93_002892"
     CDS             32474..33187
                     /locus_tag="IVK93_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP6388087.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            33207..33725
                     /gene="msrA"
                     /locus_tag="IVK93_002893"
     CDS             33207..33725
                     /gene="msrA"
                     /locus_tag="IVK93_002893"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6388088.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            33722..33949
                     /locus_tag="IVK93_002894"
     CDS             33722..33949
                     /locus_tag="IVK93_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP6388089.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            34101..34652
                     /gene="lepB"
                     /locus_tag="IVK93_002895"
     CDS             34101..34652
                     /gene="lepB"
                     /locus_tag="IVK93_002895"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6388090.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(34735..>34862)
                     /locus_tag="IVK93_002896"
     CDS             complement(34735..>34862)
                     /locus_tag="IVK93_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6388091.1"
                     /translation="DFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARKVFKRAV"
BASE COUNT        11842 a         5888 c         7353 g         9779 t
ORIGIN      
        1 ctaattgaga atcccttgca tgtttttctt aactagctta taataatttt aattaacata
       61 gtgttatatc tttttttatt tgtaattatt ataaattgtt ggggttgagt aaatggtcat
      121 gaatgctatt tcggcgatga aaaaaacaaa acagcagcta catgaagcgg ggtttaagct
      181 gacaccgcaa agagaagcta ctctgcttgt gcttttggaa aatgagaaag agcatttatc
      241 tgcagaagaa atcttctttt tagtgaaaaa aaagagtccg gaaattggac ttgctactgt
      301 ttatcgaacc cttgaaatct tgacagaatt gaaaattgtc gataaagtaa gttttaatga
      361 tggattagcc cgttatgatt tgagaaaaga aggcgccaaa catttccatc atcatttact
      421 atgcttgaac tgcggcacga tcgaggaagt agaagaggat cttttacttg aagtggagca
      481 agtagtagaa gaacgttatc attttttagt aaaagatcat cggctaacgt tccatggaat
      541 ctgtcaggaa tgctatgaaa aagaaaaaag aaaccagtcc gtctgagtgt ctagacggct
      601 ttttctttgc tcagattatg gaaaaagact atcagaatga agcggtatct gatatgatag
      661 agaggcatga gggggattat tatgaaagag caaatcaacg attatctcca ctacttgaat
      721 attgaacggg gattgtccgc caatacacga caaagctatg aacgtgattt ggaacagtac
      781 ttgcattttc tacaggagca gaaaattgct tcatggcagg atgtcgatcg ctttttagtc
      841 atccgttttc tggagaagtt gcatgaagaa aaaaaagcga cagcaactgt tactcggatg
      901 atctcaagtc tcagaagatt ccatcagttt ttacggcaag agcgtatcac tgatcatgat
      961 ccgatgcaac atatcgatac gccgaagaaa gcacagaaat taccaagtac attgactctg
     1021 acagaagtag aaaagctgat tgaagcacct gatacgacaa aaacgttagg gataagagat
     1081 cgtgcaatct tggaagtcat gtatgctact gggatgcgcg tcagtgaatt gattggattg
     1141 aaattgggag atcttcatct ttctcttgga ctggtacaga cagttggaaa aggagataaa
     1201 gagcggatca ttcctttagg tgattatgcg atccaatggc tggaacgcta cttagacgaa
     1261 gcacgtccat tacttgtaaa ggacgcatct gaaatgcatg tatttgtcaa taatcatggg
     1321 aaaggtcttt ctcggcaagg aatctggaaa aatctgaagc agttagtacg agaggctggg
     1381 atttacaaag aagtaacacc acatacgctg cggcatagct ttgccacaca tctgctagaa
     1441 aatggagctg atttgcggac agtacaggaa ttgttaggtc acgcggatat ctctacgact
     1501 cagatttata cacatatcac gaagaaaaga atgacggatg tttataagca gcattttcca
     1561 agggcttagt cataggaagg tgagaagatg ttaacacatt accggaaaga aaaccgtaaa
     1621 attgctatgg gactattgag tttccataag aaactgacag gtaaaacttc tttgttgaag
     1681 gagattgata cgtacgaatc tgaagacgat tatgaacttt ttttctttac gccagaaggt
     1741 tcaaccaaca ttcaagggat tattggtatt tactgggcaa cgaacgatca gctgatcatc
     1801 catgacattt ctttgaatcc gtcttatcgc ggtgagaata tgggctttca aatgttagat
     1861 gaattacagg aaaagtaccc ggatgtctcc atccgtgcaa ctgaaatcac agagccttac
     1921 attggaaaat ggctagcaaa gaaaaagaag aaagagtgaa tgagttgacg gagatcaata
     1981 ttaaattaga tgtattcgag ggtcctctcg atctgctgct tcatctgatt cagcaaatgg
     2041 aaatcgatat atatgatata ccgatcgcta cgatcacgga gcaatatatg aattatatcc
     2101 atacaatgaa aacactcgag ctagctgttg caggagaata cttagtgatg gctgctacat
     2161 tgatggcaat caaaagcaag acattgcttc ctgtccaaga attcgtcaca gacgatgaag
     2221 aattatggga agaagatcct cgagaagaat tggtcactca gcttttagaa taccgtaaat
     2281 ataaatatgc agctgaaaga ctgacagaaa aagcagagga acgaagtctt tattacacaa
     2341 aagaacctat gaacatcgat gggttagcgg atgctgaaaa gccgttgaag cgaggacaag
     2401 tcaaaaaaat cgatttgatc cgtgccatgc agaaaatctt agaacgtaag aaattttctc
     2461 aaaaagtgga aaaaacgatc acgcccgatg acacaacgat cgaagaacga atggtattta
     2521 tcgaagagcg tataaaaaga aatgcgcatg gctgtacttt cgattctttt tttgagtcac
     2581 cttctaaaag tgaagttgtc actactttta tggctttgct agaattgatg aaaaaagggc
     2641 agatcattgc gcaacaaaaa gaaaactatg aatcaattat gctttatcca gcagtaggag
     2701 ataccgaaca tgacgaaatt aagtgaactt gaagcccttc tttttattgt tggcgaggaa
     2761 gggatcagta atgaagaatt aagcggactg ttaaatctca gcgaagcaga agtcacaagt
     2821 ttagtgaaag aattaaaaga atcttacact caaaactcgc actcagcttt gcatattttt
     2881 gaaacggaaa gccattatgt attaacgact aaaaaagagc tggctccctt acttaagcga
     2941 tatgcgcaag ggacatcaaa cacattatct caagccgccg tcgaaacctt agcaatcatc
     3001 gcttataaac agccaatcac aagagctgaa atcgagcaga ttcgaggagt tcaggtttca
     3061 ggagcggtac agcgtctagc tgcccataaa ctgattgaag aaaaaggtag agtagaagga
     3121 ccaggtcggc cgatattata tggaacaact ggctacttct tagattattt tggtttgaaa
     3181 tcattagaag aactgcctga tacacaagaa atggaaattt cacttgaaga agacgtgtca
     3241 ccagatctat tctttgatgg aatgaataaa ggagaataat tgtaatggag agattgcaga
     3301 aagtaattgc ccacgctggc atcacttctc gtcgaaaagc agaagaattc atccttgcag
     3361 gacgtgtaaa agtaaacgga caagttgtaa gggaattagg gataaaagtt gggaaacatg
     3421 acactgtgga agtggatgaa gtcccaattt atcaagaaga atacggttac tatttattgt
     3481 acaaacctaa aggagttatt tctgctgttg cagatgacaa aggaagaaaa gttgttacgg
     3541 atcttttgcc gatggtaaaa gaacgtatct atcctgtagg tcgactggat tacgatactt
     3601 cggggatctt gctactgaca aatgatggag attttgctca gcgcctgaca catccaaaac
     3661 atgaagtgga taaagtttac gttgcaaagg taaaagggat cgctacaaaa tcgatgcttg
     3721 cacctttgac aaaaggaata aagatcgaag gacgaaaaac atctccagct aaattccaaa
     3781 ttttatcagt ggatcctaag acaaaccaca gtatcgtgga attgacgatc cacgaaggac
     3841 gtaatcatca agtgaaaaat atgcttcaag cagttggtct tcctgtgcaa aaactaaaac
     3901 gtgaaaaata cggagatctg actttacaag gcctacgtcc tggggattac cgtgaactga
     3961 acaaaaaaga agtcagcagt ttgctgaacc actcaaaatg aacacttact ttttataagt
     4021 gttagatgtt gcgttttttg aatagatatg tataatgaat atgtaaacaa aaattatata
     4081 aggttcgtct tcaggggcag ggtgaaattc ccgaccggtg gttatagtcc acgacctatt
     4141 ttgcaaaaaa atagctgaat cggtgaaatt ccgataccga cagtatagtc tggataaaga
     4201 agatagagct tatttgacga cgcttgatca aataactcta actctatttt ccctggaaaa
     4261 tagagttttt tcatttggag agaaacgtca aaaagttcgc tgaagaggag tcctaacagg
     4321 aggatttcaa atgaagaaca cccgtgtaca gaaaatggtt gcagtagcga tgtttgctgc
     4381 gatcgcatta gttttacagt attttgcttt tccgatcatg ccggctttta gttttttgaa
     4441 gatcgatttt agtgatatct cggtattgat cagcatgttc ttattcggtc cagcggccgg
     4501 agttgcaaca gcgttcttgc gttcagtatt acatctgctg actacaggtt tttctccgga
     4561 taatttggta ggagacactg ctagtttctt atcaacaacg atctttacat tcccgattta
     4621 ttatttcttt agtgtaaaga aacagcataa aggaagaaac aaatttttag gtgtcattac
     4681 aggaaccatc gctctaacta tttttatgag tatcgcaaat tatttcgtta ttacgcctct
     4741 ttacttaatg tttttagggc tgaacgcaac acagatgctg ggcatgtctt tagcaaacta
     4801 tgtactgatt gggatcgttc ctttcaactt gatcaaagga ttcatcgtga gtgctgtctt
     4861 tttagtgctg catgcgcaac ttcttccatg gttaagtcga aaacaacatc agttcgaaca
     4921 acgtcataca gcagtcaaat aaatatcaaa aagatcaaga aatttcttgg tctttttttt
     4981 tggaaaaaaa tacaagacat gcaataatgt aaacgtttta tttgtttgtt ttgagtattt
     5041 ttttgattta tgcataaaaa tcaatcaatt ctttgcaaat gtgaaattct tctcctttta
     5101 aaagacgaaa atgtttgttt ataaaaaaaa ggaaacgcta caatgaaaaa gaagaaagct
     5161 ttaaaaacct gaaaaaagag gagggaatag ggaaaaacga actttttgaa caaaagtttt
     5221 acaaaatgcg tgttattatt tttgttctga tactcttttt gataaaataa cattagagcc
     5281 aaaattgttg tctctaacgg aaaagaaagg aagaagaaga tggaacaaac aaaaaagaaa
     5341 aaatttcaaa tgccttcagc gtatacggtc ttatttatta ttattgcagt aatcgctgta
     5401 cttacttggt ttataccagc tgggcactat agtgtagacg atgcaggcaa catcgtcgct
     5461 ggatcatatg aacgagtaca acaaaatcct caagggctgt gggatatttt catggcgcca
     5521 atcaatggga tgctaggtgt accagcgggg gagctaacta aagaaacacc tgctgcaatc
     5581 ccaatctctt tcttcatttt ggttgttggt ggatttttag gtattatcaa caaaacaggg
     5641 gcactagatg ccgggattgc ttcagttgtg aagaaattca aaggaaaaga aaaaatgctt
     5701 attccagtat tgatgctgtt atttgcatta ggcggctcta cttacggaat ggcagaagaa
     5761 acgattccat tctatgcttt actgatccca gtcatgatgg ctgtaggctt tgatacagta
     5821 gtagctgtag caatcgtatt agtagggtct caagtaggct gtctagcttc aacggtaaat
     5881 ccttttgcaa caggcgtagc ttcacaagct gttgggattt cgatgggtga aggtattggt
     5941 cttcgattct tgatgttcgt tattttgtta gctatttcta ttgtatatgt gtatcgttat
     6001 gcatcaaaga tcgaaaaaga tccaacaaaa tcattagtat acaaccaacg taaagaagat
     6061 atgaagcatt tcgatattga agcaatcggt cgtcaagaag taatcacgaa aaaacaaaaa
     6121 catgttaatg tattgttctt tattactttt ggtatcatga tcatcagttt gattccttgg
     6181 acaaccttga atgcgaattt cacgattttt gaatcattga caaactggtt aacaaacctt
     6241 ccggtactag gtacggttct aggtaaaagc atgcttccat taggtgactg gtacttccca
     6301 gaaattacta tgttattctt agtaatgtcc atcgtagtag caatcactta tggcatgaaa
     6361 gagtctgaat ttgtctctga atttttagct ggtgcttctg atttgatcgg tgtagcaatc
     6421 gtagtagctg ttgctcgtgg tatccaagtg gttatgaaca atggattaat cacagacacc
     6481 atcttacatt ggggcgaaca aggattaagt tcattatctt caagtgtatt catcattatt
     6541 acatttattt tctatatccc tatgtcattc ttgattcctt caacttccgg cttagctgct
     6601 gcaacaatgg gaatcatggg acctatgggt gaatttgccg gcgtcggtaa ggatttagta
     6661 atcactgctt atcaatctgc atctggttta gtaaacttaa ttacaccaac atctgctatc
     6721 gtaatgggtg ctgtagcaat tgctcgtttc gatgtttcag tgtggtggaa atttaccggt
     6781 aaattaattg cgatattatt tgtagccatc tgtgtgatcc taggtgtagc agcaatgttc
     6841 taaacctaga caaatacagg tacaaggtct atcataagaa tgaagaaacc gataaacggc
     6901 tatcgataag cacgtggata agtcaatagc tccattcagt gagtgttttt tctttcatca
     6961 tttgttaaga acattctgct tgctgatgga gcaggcttaa aacgtcatta tttgagaaga
     7021 agaggttgtg tcggaggaat ttgacatgac cctttttaac atattaggag gataatatca
     7081 tgaaaaaatt tgtgacagaa caacatcatg aacaagcatt agaatcattg agcgaattga
     7141 tccgaattcc ttccgtacta gatgaagcag attcaggaca aggacatccc ttcggtaaaa
     7201 aagtgattga agctttggat aaagtattgg aaatcagtga aaagctagga tttagaacat
     7261 ttaaagatcc agaaggctat tacggttatt cagagattgg ctctggagat gaattattcg
     7321 gtatcttgtg tcatatggac gttgttccag caggggacga aaataattgg gaaacaaaac
     7381 cattcgatcc aactataaaa gacggctggt tagttggtcg cggatcccaa gatgacaaag
     7441 gtccttctat tgcggcaatg tatgccgtca aagctttgat tgatgctggc gtggaattca
     7501 agacacgtat tcgttttatc tttggtacag atgaagaaaa tttatggcgt tgtttagaaa
     7561 aatataacga aaaagaagaa ggaattacgc aaggatttgc gccagatgct gaatttcctt
     7621 taatttatgc agaaaaaggc ttattgcaag cttacttgac aggaccagga acgaacgagt
     7681 tttctgtaaa agctggagga gcgttgaatg ttgttcctga tgcagctcct tactctggag
     7741 aaaagctttc tgaagtaaaa gaagcattga aaaaacacga attcgatttc gaagaacaag
     7801 gtgaaggcat cgtcgttctt ggtaaaagta tccacgcgaa agatgcagca caaggagtga
     7861 atgctatttc tcgtctggca attgctcttt cagaagtatt tgattttggc ccaatcaatt
     7921 tcttaggaaa attagttcaa gaaaatgcaa ctggtgaagc tgtcgtgggc aaaacagaag
     7981 atgaacaatc cggcgagcta acgatgaact ttgcaagttt ggaaatcaca ccagaacaaa
     8041 caaaaatcgg tgtagatatg agaatccctg ttacattcaa gaaagatgat ttagtagcta
     8101 aattaactaa aacagcagaa aaatatgggt tgacttatga agaatttgac ttccttgatt
     8161 cattatacgt gccactagac agtgagttag tgaaaaatct attggggaca tatcgcgata
     8221 ttactggaga catgactgaa ccgtttgtat caggcggcgc tacttttgct cgtacgatga
     8281 accagtgtgt ggcattcggt gcgatgttcc ctgatactcc tgactttatg caccaggcga
     8341 atgaacgatg ggaattaagt agtatgtata aagcaatgga aatttatgca gaagctgtgt
     8401 atcgtttatg tgctaaataa aaataaacat acaaaaaggg cggatattca aatggatatt
     8461 ccgtcttttt ttatttgatg ctggaaagtt tgcatttctc tcttattttt ttaaggtata
     8521 atcatacttg attaagaaaa aggggtcact cagatagaaa aggtgggaga aagtgaaaat
     8581 atttagacga atactaattg gcttagtcat cattttagga ttactcggtg gagccggtta
     8641 ttactatata cagcaaaata cgtacgaacc atcgtcagaa gcgcaagtgt tggtaaaaga
     8701 aggaaaaaac aaagaagatt acttattttt tgaaggagag aaaaagaatc atggaaagtc
     8761 tccattactt attttttatc ctggtgctct cgtagagcca gaaagttata gtatttgggc
     8821 gcaagattta gcaaaaaacg gcttttcagt cgctattgtc aaaatgccac ttgatctagc
     8881 agtgatgggc ggaaacaaag cggataaggt caaagaagat tttccaaatt ccgattatgt
     8941 gatcggtggc cattcattag gtggcgtaat ggcaagtcgt tatgtcaata agaatcattc
     9001 agacaaacat ttaaaagggg tattcttcat ggcaagttat cccgacaaaa aaggaacatt
     9061 gacagaatca gctgttcctg ttttatcgat cacggcgaca aaagataaag tattgaatca
     9121 ggaagcttat caagaagcta agcagtattt accggaaaga acagagtatg tatcaatcaa
     9181 tggcggaaat catggaggtt ttggcagcta tggtgagcaa aaaggagatg gagaggcaga
     9241 aatttctaac gcagaccagc aaactgtgat cgtcaataca ttggaaaact ggctgaaaaa
     9301 gttgaaataa tataaatggg tcctataggg tccgtgaaaa aactagtcaa ttaactatag
     9361 atagcaaatg aatctttcaa aataagtcgg gatttcacgg aacaagagaa acagatcccg
     9421 gcttattttc aagtttagag aacttagtgt acgtttttaa agtttcgttt atatttatat
     9481 tttattttct aataaaatcg ctcgaactgg acatttttga tacgcttttt ttacatatgt
     9541 ttcttcttcc ggcatcacga actgttgatg agcctctggt tcttgggcaa ataaaacgat
     9601 tccttcttca tcataatcga aaatatctgg cgcttcgatt tgacaaagac cgcatgcaat
     9661 acagcgttca ggtacaagtt tacatagtag tgacatttct aaccatccta acttaaggag
     9721 aataatatga ttgtttttat tttatcgcta ttttcatcag gacacaagct gcggattagt
     9781 tctctttacc aactccttgt gggaaaacga acgacttcag tacttattta tggatttacc
     9841 cacgaacttt tgtttatcca taacagtttt ccagaattga agcaggataa gttttatcaa
     9901 attttgcaaa aaattgcgca gcaaggttgg atcgaaataa atgaaaatga agcaaagcta
     9961 acgcctgcag gggctgatat gttgtccgaa catcaaatag aacatactgg tttgcgtttt
    10021 gaccgctatg gcagaacagg ggaaactagt tggcggctca ttaaatttgc tgttcaagta
    10081 atttctaatc tggcaacggg tattcaagac tatcttccag cagagacaag tcctttttat
    10141 actttccaac taaaaaaatg gcttagtgaa agccaattgc cacgtgaaat tttgattgat
    10201 actgcgtatg agagtttggt gcagctgttt tcggagatac cagaagaagc agcagatttt
    10261 ttagctaacc aattatctgg aaatgagcga acagggctgc ttccttatca gctggcaaaa
    10321 aacaatgatg agtcagcggt ttatctgcaa caaagcagat gtatccacct tttgttagca
    10381 caaatcgaaa aacggccaga ttcgctttgg cacgctttga tcgattcatt attacagcaa
    10441 aatttcaatc aaagcatgat gatcaccaag cagatgttta tgaatggtca gacaattgac
    10501 cagatcatgg caatccgtca tttaaaaaga ggaacagtaa cagatcatct tattgagtgg
    10561 gccttgtttt ttgatgattt tccatacgag tggattctct ctgcagaaac aatcgaacgt
    10621 ttggaaccaa ataaggattc cgtgcgagag tggcgcttta gcgaatggaa tgtagatgga
    10681 caattagatt atggagaatt tcgtttgtat caaatctatc tattgagaaa ggaagcgata
    10741 cagaatgtta ataaatgaat taaaaaaata ttttggattt gattcatttc gtcccggcca
    10801 agaagaaatt attcaggcgt tgcttgatgg agaagatact ctcgctattt tgcctacagg
    10861 aacgggaaaa agcctttgct atcaattgac ggggtattta atggaaggtc tggtcatcat
    10921 cgtgtcaccg ctgctttctc taatggaaga tcaagtgacg caattacaaa aacgagggga
    10981 gaaacgagta gctgctttca atagtttact ttcgcaatct gaaagaagat acgtcctccg
    11041 tcatctatcg caatataaat ttttgttttt gagtcccgaa atgttgacta atccttctct
    11101 tttagagcat ctgaaaaaac aaaatattgc gttatatgta gtagatgagg ctcattgcgt
    11161 gtctcaatgg ggagttgatt tccgtccaga atatcaacag ttaggaaaaa tccgaaagct
    11221 tttaggaaac ccggtcacac tggcactaac ggcgacagca acagatttag tagcaagaga
    11281 tatacgtcat gtactatttg atcgaccacc aaaagagatc agacaatcag tcaatcggca
    11341 aaatatctcg ctttttgttc ggaaaacgca gcaaaaagaa caagaacttg aacacttcat
    11401 ggaaagagca caaggagcgg caatcattta ttgtgcaaca aaaaaagaag tcgaacgatt
    11461 gtatcattta ttcagggaac gttttacagt tggatactat cacggtggtc ttgatgcagc
    11521 tcaaagaaga caacttcaac agcagtttgt aaaaaatcag ctgcagtttc tcattgctac
    11581 gaatgcattt ggtatgggga tcgataaatc tgatatccgt tatgtggtcc attatgattt
    11641 gccagacagt ctagaaaact atgtacaaga aatcggtcga gcaggcagag atcaggaaga
    11701 aagtgcagca attctttttt atcaaagtgg ggatgaacgt atccattatt tctttaatca
    11761 actttctaga gaacagcgac aaagttttga gctttatttg gaatatgcca cagagcaagc
    11821 tccctttgat gagctgcaaa aaaaatggat ggagctgatc cagcagtctg aaaaaccaga
    11881 aaattgggtg gaacggctca aacgtcagga aaaagaaaaa gaattccgcc tgcagcagat
    11941 gcttcgctat attaatgaag aaaactgccg cagaaaattc atcttagctt attttggcga
    12001 aaaactctcc gaaaagccgc agaattgttg tgatattgat ggagcaaaag cagaacttct
    12061 gccaaagagg aagctttttt ctaagactga acaacttcat tgggaaacga ttttactaaa
    12121 tttatttaaa aaaaacatgg aacattgatt ttttaggaca agacactttt tcctatggta
    12181 taatacacac gatagagttt aggaggtaaa acagtgagca gaaaagatag acatcaatct
    12241 acagaaaata acgaaacaca agaaccatgg gaacaaccaa tctacgatac agatgatgaa
    12301 gtttcatcaa gaagttctca gagacaacaa aagaaaggaa atacattatt tctttcgcta
    12361 tttttgattt tactggtctt atgtatcgct attccagcag gtgcttattt ctggatcaga
    12421 aatggatcaa gcaacaatgc aacagcagca acttcttctg aaacttcgac atcagtagta
    12481 gaaagttcta caagtacaac aaaagaggag tcaactgaga cttcaacagt agaatctagt
    12541 acaatcgcgg aagaatctac tgtgtctcaa gatacaggtg taacgtcaga aagcagtatt
    12601 gcagcagaga caacaccgac ttcctcaagt gaggcaactt atcaagcagg ggattcaagc
    12661 agcaatgcag cagaaacaac accttcttca tcagctgcag ccggttcaac aacgactgtt
    12721 caagcaggcg aaggtccaaa acaagtggct gagcgtgcag ggatcaccac tgatcaattg
    12781 tttgaattga atggcttaga tccaaacaac tttatgcttt accctggtca agaattaaga
    12841 attaaataat aatagccgcg ctttgcggct ttttttctga gaaaaaggag ttatatcatg
    12901 acacaaatca gtattgcaat cgacgggcct gcttcatctg ggaaaagtac cgttgccaag
    12961 atattagcaa aagaattgaa ttatatttat acagataccg gtgccatgta tcgcgcagta
    13021 acttatttag ctattacgaa caatgtatct ttttcagatg aagaagggct agtttcgctg
    13081 atcaaaaagt atcctatctc tttttcgcaa caagaagatg gacagcatgt attcatagca
    13141 tcaaaagatg tgactttaga aatccgtcaa ccagatgtca caaaagctgt ttctgaagtc
    13201 tctgcacatg gaaaagtcag agaagaatta gttgctgtcc aacgaaaaat aggggaacgc
    13261 ggtggcgttg tcatggacgg aagagacatc gggacagctg tattaccgaa tgcagaagtg
    13321 aaaatctttt tagtagcaag cgtaagtgag cgtgcagaaa gacgtttcaa agagaaccaa
    13381 gaaaaaggaa ttccgactga tcttgaaaca ctgacgaaag agatacagga aagagaccat
    13441 tatgattcaa caagggacgt ctctccgctg aagcaagcag aggatgctgt tcgtatcgat
    13501 acgactggca aatcgattcc agaagtcgtt gcagcaataa aagctgttgt tttgaaaaaa
    13561 ggttatcttc ttttttaaat taagaaaaaa aatgaaaaac aattgttttt tcaaggaaaa
    13621 cgaacgaaat cgctttattt tttttgaata atcaattaaa ctaaaaacag taccgtactt
    13681 acggcagaat gttggttagg aggacaagtg ttatgacaga atttgatcaa aatcaagaga
    13741 acaaccagtc aatggaagac gcaatgaaaa gcgtacaaga agtaaaagtt ggcgatgttg
    13801 ttaagggcga agttttagtc attgaagaca aacaagtgat tgtcggaatc gaaggggctg
    13861 gagttgaagg agtcgttccg gcaaaagaat tatcaacttt gccggtagaa gatatcagcg
    13921 aagtagtaaa agtcggtgat gtattagatt tagtcgttat cagtacgatc ggcaaagata
    13981 aagaaaacgg cagctatcta ctttcaaaac gccgtttaga tgctaaaaaa gtttgggaag
    14041 atatcgaaag agatttccaa gagggcaagg tcatcgaagc acctgttaca aatgttgtca
    14101 aaggcggttt agtcgttgac gtcggtgtac gtggttttgt ccctgcatca atggtggaag
    14161 atcattttgt tgcagacttt tcagactata aaggcaaaac attgagcttt aaaatcatcg
    14221 aaatcgaacc atcagaaaat cgtttgatct tatcgcataa agcggtagtg gaaaacgaaa
    14281 gagaagaaaa gaaaaaagaa attctttcaa ctatccatga gggagatgtt ctcagtggac
    14341 gtgttgcacg tcttactgat tttggcgcat ttgtcgacct aggcggaatt gacggtctag
    14401 tccatgtctc agagatttca catagtcatg tagcaaaacc aagtgatgta ttggctgtca
    14461 atgatgatgt aaatgtcaaa gtgttgtcta tcaatcctga acaagaacgt gtttccttgt
    14521 caatcaaaga cacacagcct ggaccttggt cagaaatcga tacaaaagct ccagttggca
    14581 cagtattaga cggaacagtt aaacgtttga caagcttcgg tgcctttgtt gaagtgttcc
    14641 ctggagtaga agggctagtc catatctctc aaatttcaca taaacatatt gcgacaccac
    14701 atgaagtgct tcatgaaggt gacgaagtga aagtgaaagt attagagatc catccagaag
    14761 aacatcgtat tgcattgagt atcaaagctt tagaagaaaa accagcttca gaagaagaac
    14821 caaaaaatgt tgagtcttat gaattaccag aagaaaatac tggatttact atgggagaca
    14881 ttttaggtga tgcgttgaaa gaagaagtag aagaaaaagc aaccgatgat tctgaaaaat
    14941 aattgatttt gtatctgtga ctgagatgaa agcagctgct tttgtctcag tttttctttt
    15001 gaaaagcgat tagtatttca ataaggaata aaaatacttc gtttaattga caatctatgc
    15061 ttgaaagttg gcaatgcatt cgttaaacta agtgagattg gaaacttgtg aggaggaaat
    15121 aatatggcaa atccaacaat tgcaatcgtt ggccgcccga acgtcggtaa gtctacgatt
    15181 tttaaccgca tcgctggtga gcgtatctcg attgtcgaag atactcctgg agtaactcgt
    15241 gatcgtattt atgcaaaagg tgaatggtta ggtcgagaat tcagcgtgat cgacacaggc
    15301 ggaattgatc ttggcgatga accttttatg gatcagatca agcatcaagc agaaatcgcg
    15361 attgaagaag cagatgtaat catttgtgta gtcagcggac gcgaaggcgt gacagacgca
    15421 gatgagatgg ttgcgaaaat tttatacaga agtaataaac cagtgatttt agcagtcaat
    15481 aaagtagata atccagaaat gagaaacgat atctatgaat tttattcctt agggctaggc
    15541 gatccttatc ctgtttcagg aagccatgga ctagggattg gtgatatttt agatgaagca
    15601 gtgaagtatt tctctgaaga gactgaagaa gaggacgacg acacgattaa gtttagtttg
    15661 attggtcgcc caaatgttgg taaatcctct ttgatcaatg cgattttagg agaagaacgt
    15721 gtaattgttt cggatattga aggaacgacc cgagacgcaa tcgacaccca cttcgtttct
    15781 gaaagtggtc agaaattctt aatgatagat acagctggta tgcgtaaacg cggaaaagta
    15841 tacgaaaata ctgaaaaata cagcgtgatg cgtgcaatgc gtgccattga ccgttctgat
    15901 atcgttttga tggtactgaa tgctgaagaa gggattcggg aacaagacaa gcgagtagcg
    15961 ggttacgccc atgaagctgg tcgtggaatt attatcgtcg tgaataaatg ggatcttgta
    16021 aagaaagaaa ccaatacaat gcgtgatttc gaacaagaga ttcgtgatga attccggtat
    16081 cttgattacg ctccaatcgt ttttgtttct gcagtgacta aacaacggtt ggaacgtctg
    16141 cctgaaatga tcgaacaagt aagtatgaac cagaatcttc gtatctcttc ggctgtgttg
    16201 aatgatatca ttatggatgc tgtagcaatc aatccaacac caacagataa aggaaaacgc
    16261 ctgaagattt tttatgctac acaagtcgct gtcaaaccac ctacatttgt cgtttttgtc
    16321 aatgaagagg aattgatgca tttttcttat gaacgtttct tagaaaatca aatcagaaaa
    16381 gcatttactt ttgaagggac gtccatccga attatccctc gtaggagaaa ataggcattt
    16441 taccatatcg acgttttttt ttcaaaagaa aaagacagtt tcgtttgtta cgtttctatg
    16501 actaaaaaaa gcatgtgaaa attggtaaaa accttgctat taagcggttc atatgatatc
    16561 gtgataacag aatattgaag ttaatcaata tttcttttta aacttctgga ccactcagaa
    16621 agttttaact aaatgatatt tctatcatat ctcttgtgga ggaggtgaat ctatccatgg
    16681 caaacaaagc agaattaatc gaaaaagttg cttcagctac tgatttaact aaaaaagatg
    16741 caactgcagc agtagatgct gtattttcaa ctattcaaga tgctttagct aacggcgaaa
    16801 aagttcaatt aatcggtttt ggtaactttg aagttcgcga acgcgcagct cgcaaaggtc
    16861 gtaacccaca aactggtgaa gaaatcgaaa ttccagctag caaagtacct gcattcaaac
    16921 caggtaaagc attaaaagac gctgttaaat aagacagttg aaaaagcgct tgtggctcta
    16981 gggctacaag tgcttttctt tttatttcct agcagaaaag gaataaataa gagacagaga
    17041 ttccgaatcc gtatgtcata catagaaaac acatgtattt tatttaattt ttatttattt
    17101 gcaagttatg aggtgagctt tgctcttatc ttttctagaa tttgcttact aatggtaaaa
    17161 taaaaataag aagaggaggg atgcgtatgg aacttgatta tcaagtagaa gatgcgttga
    17221 cgaaagtgtt ttacggagaa acgctagtca gtcagctaag agaactttcc attgatcagc
    17281 caatcttatt tttaacgaat cagcgctatt atgatttgtt tgcagataag atcaacccgt
    17341 tatttgcaaa tcaagcaaac aatgactggt atatttgtgc caacactcaa tgcaatcatt
    17401 taacagaatt aaagaacctg cttgatttcg tcaaacgcta tcccgaaaat caatccatgt
    17461 ttatcatcgg atttggaaat gaagggatca tggatctggc aggttttttt caaaaacata
    17521 caacccttga ttcatcacta tggatgatcc ctgtatcgat ccgttctcta agccgagcat
    17581 taacaccgca gcggacgatt ttgcagctac caaatcgtgt agtgctgcaa accatcaatc
    17641 tgccagaacg catcgtttat gatcaaacaa tcagcgagcg gcaggtggac ggaaagcaga
    17701 ttgacttttt gacttttatc tgttgcggaa tgatttgtga tcatcgtttt ttgcaaaatc
    17761 tgtataaaaa ctatccaaat aaaaagcaat taacgactcg gccgtttgcc gggatgatcg
    17821 aagaaatgat ctatttttat caattagagg cagaaaacct gtcaaattat ggtaaactgt
    17881 ttgaacaagc tttttatctg accgaaaatg gccatctact ttctgctagt atgaaaaaat
    17941 ttttaggaat gatgtttcat ttcgtgtgga atctccaagc atatcctttt gatttccaac
    18001 taaaaaactt ttttgtgtgg ctggacttgc ttggctatcc aatccactgg ctggaacaga
    18061 tttcaaagat ggagtatttg gaaaaagttc tttttttggc agaaaaaagc aaaaaaatac
    18121 ttgttttaga aaaaataggt ataatagacg gataccaaca tccaaacgaa aaagaattat
    18181 tgagaacaat gacggtattt caacaaatcg tcagtgagat aagagggaat tgaaatgaat
    18241 acaaatagcg aaaaaatgtt gcaagcttta tcagatgaag acttgtcaca agcacaaatc
    18301 tttttagaaa aagcattaaa agaagaccca gcagatatat tagctgaact aggcgaagaa
    18361 ttgctggcta ttggcttttt gatcgaagca aaacagattt ttgaacaact gatcaaccaa
    18421 tacccagaaa atgacggact gaacatcccg ttagcagaaa ttgcaatcga agataatgag
    18481 atcgacttag cttttgaata cctggaaaaa gtggataagc aaagtgatta ctatccgcaa
    18541 agcttattgg cgatagctga tttgtaccaa gttatcggaa tccctgaagt gagtgaagca
    18601 aaattgaaag aagcagctgg gttgcttcct gatgaaccat tgatccagtt tgcgctagct
    18661 gaattgtatt tttcagttga tcgtttcaaa gaggcagcag tggtttatga aacattgttg
    18721 gcaagcggga taaatgaaat ttcaggaatc tcgatgcagg aacgtttagg acaatcctta
    18781 agtatgcaag gagaattcga agcagctatc gtacctttag aagaagcact gaaagaagaa
    18841 aggacagatg atcgactgtt ccagctagct tttacgaatc ttcaattgaa agaaaatcag
    18901 caggcaatca actatctgga agaacttcga gaagttaatc cgcattatca atcgttatat
    18961 ctatatctag gacaagctct tcaagaagaa gaaatgattg aagaagcaca aacagtgtta
    19021 gaagaaggaa tcaaagaaaa tccttaccaa gtcgaacttt atcacctggc ctcggaaaat
    19081 gctttccgtc tgcatgatag acagcgagca gaagaacttt tactgaaagc tttagaagta
    19141 ggagacaaac aagatgaaac attacttact ttaagtaatc tttacttgga tgatgaaaga
    19201 tatgaagatg tgatacaagt gatcaatcag atggaagaaa ctgctaatcc ttatgcagaa
    19261 tggaacctag cccatgctta taatgaatta gaagacttta ccgttgcagc tgtacactat
    19321 gaacaggctt atcatgaact atcacatgaa ccagatttct tgaaagagta tgcctttttc
    19381 ttaagagaag aagggcagct gaaaagagcg caagaattat tgacttatta tttaacactt
    19441 gaaccaggag atatggaggc actgtcatta ttagatgacc taacagagag gtgagaaaaa
    19501 tgctaattga tgtacgggaa aagaagaatt ttttaacgtg gatggtcaat catgtatctt
    19561 ttagccgaag agaagttttt tggatcttga attatcttgc caatcatgaa gcgattttaa
    19621 gcaatgtcca ctttgttgaa ggggcgaatt ttgcggatcg aggactgcag atcagtgatc
    19681 tatctgttga aggggaaccc atgaaattgt ttttgcaggg aaaagaattt acggacacag
    19741 accagatttt tcatgaaatc cgtttgaatt ggaaaaagcc tttatatgtg gaatgtatat
    19801 tcgaaaatgc ttggcagaca agagaatatc tatctatctt agaagacaat ccattttctt
    19861 cttgggattc ttcagtcagt gaagaagata ctgaattaat tgaacgatat tttcaagaaa
    19921 aagaagaaga ggcaaaacta aaaatgcttt atgctcaaat cgatcaagca ctagaagatg
    19981 gagataggga tgcttttttg aaattatctg atgaagttaa ccgaaagatc cttttgcaga
    20041 cacaaaagaa aagtaaccgg gtaagtgaac ataacaatta gttaaaaata gtatagtact
    20101 aaaaaagaca aaggtcagct ggatgtggaa tctttcctca tagcctatct ttgtcttttt
    20161 tcttatagtt ttttcagtaa tcttgtctgt ggcttcaaat aagtaaagcc ttgcccttcc
    20221 acttcatgca cattgatgat tgaaaggaaa gctttttcgt caatctcgtg gacgatcctc
    20281 ttagcttcag ttaattcact agagctaacg accatataga tgattttttt tgaattacca
    20341 gagaatgcgc cttctccaga aaggaaagtc acacctcttt gcagaccggt catcagtagt
    20401 ggtgcaatct cttcgctttt gttggagacg acaagtagac cttttgcaga atatccgcca
    20461 tctaagatcg aatcaatcac acgactgaaa acatacgaaa caatcaacgt atacatcata
    20521 cgtttcaagt cgatataggt taatgaagca agtaagacga tgatatcaaa tgccaatagg
    20581 gaacgcccca tagtgatgcc gtaattttgt tctaaaatcc gagcgataat atcactgccg
    20641 cctgttgttc cacccatgcg gtagacgatc ccgcttccta cacctgcaac taaacccgca
    20701 agtagagaaa cgatcagtag atcatgctgt aaattgattt ctagtggaac cctttgccag
    20761 atccataaaa agaatgacaa agaaagtgta cctagaacag tataggccaa ggaacgttta
    20821 ccgaggatct ttcctcctag taaaatcaat gggatgttga ttaataaagt cgtataagct
    20881 ggatcaatac caaataaagc tcttaagatc aaggtgatcc ctgagactcc cccttctgct
    20941 aaatgattag cgatgttcaa gtaaacaaga ccaaatgcat aaatacttgt tcctataatg
    21001 ataaaaagaa cttcttttac tgtttttcgt ttgaccatat gaattccagc tttcaattat
    21061 gtattttcac agaatttcca tttagaaatt ccttcctaaa tgtagcatgg aaattcgatt
    21121 ttctcaattg ctcttatcta ggatttgcgt taaagttttg gtaagatagt tttggaggga
    21181 aaaagatgga taaaaagcgt tctttataca gtatgcagca ggaagtggat gaatatatcc
    21241 agcaattcaa ggtggggtat ttctctccat tagctcagat ggctcgatta actgaagaag
    21301 taggtgaatt ggcaagagaa gtcaaccaca cttatggaga aaaaagcaaa aaggcttctg
    21361 aaccagttaa ctcggtagca gaagaacttg gtgatgtttt attcgttacg atgatcatgg
    21421 ctaattcatt gaatatcgac ttaactgaag tttttgaaaa aaacatggaa aaattcaatc
    21481 aaagagacca taatcgcttt gagcgtaagg acgagtaaac aagatgaagt ggtcctggcc
    21541 ttgaagactc tgaaacggac ttgtcaaaca gtcaccatgc aactgagtct ttatctcagg
    21601 acaagaaaca cgcaggaaag gttggaaaaa tgaaattaaa acaattacct ctggaatacc
    21661 aaaaggcaat tccagttctg aaaaaattag agaatgcagg atatgaagct tattttgtag
    21721 gtggaagtgt tcgtgatatc ttgctgaacc agccaatcca tgatgtggat atcgcaacaa
    21781 gcgcatttcc agaagaaatc aaacagcttt ttccaaaaac gatagatatt gggatcgaac
    21841 atggaactgt gttggtatta gaaaatgatg aacaatatga gatcaccaca tttcggacgg
    21901 aatcaactta tcaggactac cgtagaccag atcatgtcga attcgttcgt tcgttggcag
    21961 aagatttgaa aagaagagat tttacgatca atgcgtttgc tttgaaagaa gatggagaaa
    22021 tcatcgattt gttcgatggt ctgcaagatc tagaagaaca gatcttgcga gctgtaggca
    22081 atcctcatga gcggttccat gaagatgcct tacgaatgat gcgcgggttg cgttttgtca
    22141 gtcaactagg gttcaaatta gaggaagaaa ccttctacgc catcaaagaa aaccacgagc
    22201 tattaggtaa aatatctgta gaacgtataa atgtggaatt catcaagatg ctcttgggaa
    22261 atgaacgggc cgcgggaatc catgcattta ttgaaacaga gtgctacatt tgctgtcccg
    22321 gtttgaaaga agaaggcgaa gcacttttac gttttgctga attaccatcc gtcccattga
    22381 aagaagagag ccaagcatgg gcattattga tcagccaact ggatctaaaa gaaaatgaga
    22441 ttcgttcctt tttgaaatcg tggaagtgtt ctaaccaaat gattaaagac gttcaagcac
    22501 ttgtccaagg actacgacaa cgagaaactg gagctttgga accattcgaa ttgtacttct
    22561 taggcaaaaa acatgcactg caagtagaag aattgatgat ctattttgac gaaacacctc
    22621 agatggatgc cgtaggagaa tcctatgatc gtttacctat caagtcatta agcgaattag
    22681 cagtagatgg taaaatcttg ctagaagaaa ctggaaaaaa acctggtaag tgggttagtg
    22741 aagctttgca attagctgaa aaagccgtcg tagaacaaag aataaaaaat gataaaaaac
    22801 aggttttgga ttttttatcg aaagaaaaat tgatttaaaa aacactagac tgtatctgct
    22861 cagctttgtt ggctacttaa agtgggcaga tacgtcatta gaagaaaaaa cataagatgt
    22921 gaccacttac ataaatttgt gctaaactga ggtagtacaa gaataaatta catcataaag
    22981 gagtcttttt aatgagtaaa gaaatgacag cgctaaaatt ttattttaga aatggagaaa
    23041 cctggacgat caaccgccgt catatcggcg atctttggat caaacaaatc acaacaagtt
    23101 ttggacgaat caatggcagt gaatttgttg aaatccatcc gtgtgctgga tttaaaatcg
    23161 aaatcttcca tgaaggggac gcagttgcga cacatgatat caacctaggc ggactggaaa
    23221 tgggcatgtt taatcgcgca ttgaaatacg aagatatcga gcgaatggaa atcctttacc
    23281 gaaatggcac acctgatctt gtgtacttcc catatctaga taaaggaaca gaaggattag
    23341 acaatcaata tcaatcaaca aaaatcagcg aaaaaacagg taacttgtat atcgtgatca
    23401 atccagatca acgtgtagaa gatgtatacg gggaattttt cgaataaatc gatttttacg
    23461 caaaaatgag gctgtgaaag agatgtgtct tattactact gataggctgt ctcttccaca
    23521 ccgtttattc agaataaggg actgtgacaa gatttttgtc gcagccccat ttttttgttg
    23581 tgataaaaga acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt
    23641 atctttctta agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa
    23701 atttatggaa atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa
    23761 aagaactttg ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt
    23821 tttgaacaac aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc
    23881 cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt
    23941 ctgaaacatg atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac
    24001 caaacacagg ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa
    24061 atgcttacac aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt
    24121 gtatttgcag taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca
    24181 gctccactga tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca
    24241 atcgattttg aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa
    24301 ggagaaaaag cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca
    24361 tttggaggag tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca
    24421 agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta
    24481 gcagtcagtt ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa
    24541 ggagaaggtc ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg
    24601 ctgatcaccg gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt
    24661 gggggagctt tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc
    24721 gaaaaagcat cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt
    24781 ctagcctatg tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca
    24841 ggtattctcc aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc
    24901 ttgtcccaca gtacatggac aactattgct tttacactga atgcattagt atttatattt
    24961 ttaggaatcg aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac
    25021 ggtcttttat tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt
    25081 tctatcagtc ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac
    25141 gagatcttga tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt
    25201 attttgccat tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg
    25261 acggcgggtg tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg
    25321 gaaacagagg aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt
    25381 gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact
    25441 gaagcagtga tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc
    25501 gatgatgaaa agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat
    25561 ggattggatg aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc
    25621 cgttttcttt ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt
    25681 tggttcattg ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa
    25741 agaagatatg ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag
    25801 acatatcaaa aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat
    25861 gatgaaacga tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac
    25921 tcggggaatt tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg
    25981 ctaagaggtt attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc
    26041 acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg
    26101 aacaaaccat ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg
    26161 agtccaataa aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga
    26221 acaggatcga atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa
    26281 attttcctga agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg
    26341 aaagaattaa aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag
    26401 atttcttttt tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt
    26461 aaaacaagtc tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca
    26521 gtgttttatc caactgatta ccgtattggt tatctttctc aagcagctga attttccgat
    26581 gaattaactg ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga
    26641 gcatatgagg aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa
    26701 cgttatgcta aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac
    26761 gcaaagatta ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta
    26821 tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta
    26881 ctattactag acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac
    26941 tatttgaaac aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat
    27001 agagtagcaa accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat
    27061 tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa
    27121 aagcgcaagc ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt
    27181 ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa
    27241 gtaaaccaag aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta
    27301 ctggaaatca aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat
    27361 ttattgattc aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg
    27421 acattgctta atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt
    27481 gaaacggttc atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg
    27541 atgattgctt acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc
    27601 ggagtagcag aattacttga acgattttta tttccaagat ttatgcatgg gacactgata
    27661 ggcaaacttt caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa
    27721 cctaatgttt tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt
    27781 ttggaagatt attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat
    27841 ttcttggaca aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt
    27901 tattacggaa acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa
    27961 aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg
    28021 gaacaaaaag aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca
    28081 ttgctccaag aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa
    28141 aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg
    28201 agtgaatggg tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa
    28261 gaaaattgtt ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct
    28321 tattcggata ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac
    28381 gagtaccttt tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata
    28441 tccgctatct attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca
    28501 tcaaaagtcc agactataat ggaccagata tgactgattt tggccataga tgtttgacag
    28561 atcctgaatt caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa
    28621 ccgatgattc ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc
    28681 gtcactggga aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa
    28741 tcaaaaagac tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc
    28801 ctagcatggc tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc
    28861 taagctgcca attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg
    28921 caagttatgc gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat
    28981 ttgttcacac attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac
    29041 aattgagccg agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag
    29101 tatttgattt tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca
    29161 aagctcctat cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca
    29221 caggatgaaa acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat
    29281 ctgaaattct tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc
    29341 gaagggatgg gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct
    29401 tcctataaag cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa
    29461 aaagaaacag acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg
    29521 ccattttgta cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat
    29581 tttccacctg ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat
    29641 gaaaaaaatc gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa
    29701 agcgggacag gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag
    29761 ctgtcccatc catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata
    29821 aaacctgcac ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc
    29881 aagctataaa agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca
    29941 ccagtcattc caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata
    30001 aaaatataga tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct
    30061 ttatatataa tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc
    30121 catcctcgga tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc
    30181 gaactatgat cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac
    30241 aaagtagacg ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata
    30301 tggagggagg aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc
    30361 aatccagtcc caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt
    30421 cgattaaatt tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa
    30481 tcattatttg tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata
    30541 aggcatcttg ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga
    30601 ttcttcatgt acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct
    30661 atccatcatg atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt
    30721 tatggaaatg atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga
    30781 attcatctcg atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat
    30841 gacaaaaggc ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac
    30901 gaaagtaacg gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg
    30961 agcagctgaa ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa
    31021 agtacaagac aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg
    31081 tggaagaatc agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat
    31141 gaatttcgcc cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga
    31201 taaatggttt aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg
    31261 tatttcacat gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt
    31321 attcccggac atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg
    31381 taaagacgcg tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag
    31441 cctgaaaggc ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc
    31501 ttttttcata aagactacct ttttgataaa atttttgata acattagaac caatcgaata
    31561 caagaggtga aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat
    31621 taattgcgat catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa
    31681 tgaaaccagt aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag
    31741 ctatcggaga ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc
    31801 cccttgtcgc aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact
    31861 atggtgtaaa tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa
    31921 ttcgaaacaa tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga
    31981 tgaaagttat ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga
    32041 aaaactatca ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg
    32101 cacctattta cattttaggc atctacaatc cattttattt gaactttcct gagatcactg
    32161 acatgcagac aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc
    32221 cgaactgcta ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag
    32281 gtgtcacaga agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca
    32341 aaaataatgt gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga
    32401 tggcaaatgc agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg
    32461 atgatcaata aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa
    32521 ttactggaaa ttcgccttcc ttatccttct tggggtagta cttggttcga ccgtattttt
    32581 aggcagccgt atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga
    32641 aagacaagga gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat
    32701 cagctttttc cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga
    32761 aaatgaagca ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt
    32821 atattttgat ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc
    32881 tatcggaaca ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa
    32941 attaccagat tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca
    33001 attccgaatg caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga
    33061 cattcggttt agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac
    33121 caacaaaact agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga
    33181 tgagtagtat tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct
    33241 ggtgtatgat ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata
    33301 ctggagggca tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata
    33361 cagaagcagt ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac
    33421 tttactggca gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca
    33481 attatcgccc ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta
    33541 agaaacagtt agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag
    33601 cccaaccatt ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa
    33661 attttaaaag aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag
    33721 ggtgaatcga agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc
    33781 tttatctgtt ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag
    33841 ctacgaagaa atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat
    33901 ctttgacgaa gcatgggaaa aatatttaga aaataatcat gacatataag agatagtttt
    33961 gtactttatg atcaaaaaag ttttttgtcc aatagcctaa ttgaaagaat tatttttatt
    34021 tcagctatct agtcgcattt ttttgatatt tttcttataa taaagaaaga atgaaaaaaa
    34081 gagggatgga ttgatagaga ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg
    34141 tattcaaata cttacttgtt tctgtattcc ttgcgttcat gttgcgggga tttttattca
    34201 tcccggtacc agtggaaggc aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg
    34261 tgatggaaaa attttcggag atcagacgat ttgatattgt tgtttttcaa ttagcagatg
    34321 gcacgattta tatcaaacga gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg
    34381 atcaattgaa gatcaatggg aaagtagtta aggaacctta tctaactaaa aacctgaaat
    34441 cagatcatgc aaatgcatcc tatacgacgg actttacact acaagagcta acgggacaat
    34501 ccaagcttcc ggaagataat tattttgtac ttggagataa tcgaagagtt tcaaaagata
    34561 gtcgttcgtt tggcacgatc aataaaactg atattctagg taaagcaaga tttgtctatt
    34621 atccacttga tgaaataaaa tggatccagt gagaaacaat aaagcgtgca gtctgttgag
    34681 actgcacgct ttattgttta atccaaaatc agtttactac cttagtacgt aaaatcaaac
    34741 agctcgttta aacacttttc tagcctctaa agttttgata aatgaataaa caaccattat
    34801 ttgaataggg atttggatca tattcctgat gaatctacct agaaaaatta ctttgaaatc
    34861 tg
//
LOCUS       DACCCR010000247        23728 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.038, whole genome shotgun sequence.
ACCESSION   DACCCR010000247 DACCCR010000000
VERSION     DACCCR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.038"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..212
                     /locus_tag="IVK93_002897"
     CDS             <1..212
                     /locus_tag="IVK93_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6388092.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
     gene            387..683
                     /locus_tag="IVK93_002898"
     CDS             387..683
                     /locus_tag="IVK93_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388093.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="IVK93_002899"
     CDS             690..2642
                     /locus_tag="IVK93_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP6388094.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="IVK93_002900"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="IVK93_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP6388095.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3573..4613
                     /locus_tag="IVK93_002901"
     CDS             3573..4613
                     /locus_tag="IVK93_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP6388096.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4711..4899
                     /locus_tag="IVK93_002902"
     CDS             4711..4899
                     /locus_tag="IVK93_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6388097.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4957..5310)
                     /locus_tag="IVK93_002903"
     CDS             complement(4957..5310)
                     /locus_tag="IVK93_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6388098.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5841..6263
                     /locus_tag="IVK93_002904"
     CDS             5841..6263
                     /locus_tag="IVK93_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6388099.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6260..6490
                     /locus_tag="IVK93_002905"
     CDS             6260..6490
                     /locus_tag="IVK93_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6388100.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6988..7188
                     /locus_tag="IVK93_002906"
     CDS             6988..7188
                     /locus_tag="IVK93_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6388101.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7215..8408
                     /locus_tag="IVK93_002907"
     CDS             7215..8408
                     /locus_tag="IVK93_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6388102.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8476..10041)
                     /gene="guaA"
                     /locus_tag="IVK93_002908"
     CDS             complement(8476..10041)
                     /gene="guaA"
                     /locus_tag="IVK93_002908"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6388103.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10100..11023)
                     /locus_tag="IVK93_002909"
     CDS             complement(10100..11023)
                     /locus_tag="IVK93_002909"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP6388104.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11219..11824
                     /locus_tag="IVK93_002910"
     CDS             11219..11824
                     /locus_tag="IVK93_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6388105.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12132..13148
                     /locus_tag="IVK93_002911"
     CDS             12132..13148
                     /locus_tag="IVK93_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6388106.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13289..14590
                     /locus_tag="IVK93_002912"
     CDS             13289..14590
                     /locus_tag="IVK93_002912"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP6388107.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14636..15298
                     /gene="deoC"
                     /locus_tag="IVK93_002913"
     CDS             14636..15298
                     /gene="deoC"
                     /locus_tag="IVK93_002913"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP6388108.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15319..15717
                     /locus_tag="IVK93_002914"
     CDS             15319..15717
                     /locus_tag="IVK93_002914"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP6388109.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15783..16865
                     /locus_tag="IVK93_002915"
     CDS             15783..16865
                     /locus_tag="IVK93_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6388110.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17077..18639
                     /locus_tag="IVK93_002916"
     CDS             17077..18639
                     /locus_tag="IVK93_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6388111.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18632..19765
                     /locus_tag="IVK93_002917"
     CDS             18632..19765
                     /locus_tag="IVK93_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6388112.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19765..20721
                     /locus_tag="IVK93_002918"
     CDS             19765..20721
                     /locus_tag="IVK93_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6388113.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20820..21986
                     /gene="deoB"
                     /locus_tag="IVK93_002919"
     CDS             20820..21986
                     /gene="deoB"
                     /locus_tag="IVK93_002919"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP6388114.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22010..22828
                     /locus_tag="IVK93_002920"
     CDS             22010..22828
                     /locus_tag="IVK93_002920"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6388115.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22845..23552
                     /gene="deoD"
                     /locus_tag="IVK93_002921"
     CDS             22845..23552
                     /gene="deoD"
                     /locus_tag="IVK93_002921"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6388116.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         7720 a         4202 c         4949 g         6857 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg
     4261 tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg
     4381 caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg
     4441 ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt
     4501 gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc
     4561 gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt
     4621 tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt taatatagat
     4681 ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat attgtgtcct
     4741 atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa aaatttccct
     4801 ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat gaatataata
     4861 acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg aaactctcat
     4921 cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat ttcttgcttc
     4981 attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa agctatccat
     5041 gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag gtaagaaaaa
     5101 ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg tacttgggtg
     5161 ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca ttgttcccac
     5221 ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac caacggctct
     5281 aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat tttactgttc
     5341 ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt tcctatagaa
     5401 cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg ctgattttat
     5461 ttacctatac aactttataa aatttagtca tactcctaga aagtagataa aattggcgac
     5521 ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct cttaattaag
     5581 ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa tgtggttttg
     5641 cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct gtttatgtgc
     5701 ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca acaaatcccc
     5761 cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact ccctttcacg
     5821 acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata gaaaatcagt
     5881 ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc aaacatctag
     5941 acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt gtcaactctt
     6001 tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt ggattaagaa
     6061 tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag aaaaaacgga
     6121 acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg gagttactaa
     6181 atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg attaaagaat
     6241 tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc ccctcattgt
     6301 aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt atcgagggta
     6361 tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc atatggttat
     6421 tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt tatcgttcga
     6481 aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc ttcccgattt
     6541 gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga taggctaaca
     6601 agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt aagaaaagag
     6661 ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt agtaattcga
     6721 aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga tactcccgta
     6781 tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca tgagaaccat
     6841 gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga tattcttctc
     6901 ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt ggagttttcc
     6961 tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga tttagttgag
     7021 ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga attgatgatt
     7081 acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg tgaagctgtt
     7141 gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt tttgcacagt
     7201 aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga acctattatt
     7261 ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat cgtagtggat
     7321 tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg aaaccagaag
     7381 aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa attttccttc
     7441 catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca actattaaga
     7501 agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt gatgtgcaaa
     7561 attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct gttcaaggac
     7621 tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca aatccatcta
     7681 agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca aaagaagaat
     7741 ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt ttatttatct
     7801 ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc attcaatggg
     7861 aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat tacaagaatc
     7921 aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat attgtattag
     7981 ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat ctaattcaga
     8041 cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta tcatatgcta
     8101 ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg ttacggcatt
     8161 ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa gatcgtctag
     8221 gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct aacagtaatt
     8281 tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa caaaattatg
     8341 atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa atgaaaaaag
     8401 tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat aagaggattt
     8461 ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag tgatgtcata
     8521 cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt tttgcaagac
     8581 ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta cagcacggat
     8641 accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc ggatccctgg
     8701 aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga tttcttcacg
     8761 caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt cgccaagaac
     8821 acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat caggcatgcc
     8881 taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg gttcgatcaa
     8941 ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga tcgtctgtgc
     9001 tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta ggaaagagac
     9061 gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga attcattccc
     9121 aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta agaagcgatc
     9181 tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt ccattacttg
     9241 ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca attggtcacc
     9301 gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg aaagaccaag
     9361 caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat cgataaagtt
     9421 atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc gcagcaattc
     9481 gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa aattacgtgt
     9541 tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt caaagccttc
     9601 aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt ttggtgtttc
     9661 tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat attcgcggtt
     9721 tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac catagcaaat
     9781 ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg cattctcatc
     9841 atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg cttttacttc
     9901 ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac gaatacgtct
     9961 tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt caacgttcgt
    10021 catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta tctcaaaata
    10081 gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg atatgatttt
    10141 cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat tcctgtaaat
    10201 ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct tcacgatctc
    10261 cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc agtaacgcac
    10321 caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca tagacagaaa
    10381 aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc gtattgatcc
    10441 cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga atgatatcat
    10501 acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt acacggttca
    10561 agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg tccatgattc
    10621 cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc agctgaacgt
    10681 ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt gtactttttc
    10741 caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg tatttatgaa
    10801 gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga taaatcagat
    10861 gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca ttcaaacttt
    10921 tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa aattctcgcc
    10981 attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc tcctgcgttc
    11041 tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact taagagagat
    11101 tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt cttgacttgc
    11161 gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga tggaagacat
    11221 gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac aatgggcttt
    11281 tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt cacgtgaaac
    11341 agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt tacctgatga
    11401 aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt tggcatttgc
    11461 gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc tagcgcaagg
    11521 aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata tctatgaagc
    11581 agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg ctggaaaaaa
    11641 agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg gtacactgac
    11701 aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg aagaaacatt
    11761 tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa gtgtgaaaga
    11821 ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc atgtacgggt
    11881 acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt ttttcttcaa
    11941 aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag atacagaact
    12001 tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa catactttcc
    12061 ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt agatagaagg
    12121 gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc gtatcagtaa
    12181 caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag acaagaaaaa
    12241 aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct tcaaatgtga
    12301 tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat ttcttcttct
    12361 ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg atcatgttat
    12421 gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt tcccaccgct
    12481 cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat gcattagaca
    12541 gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat cgtcgggata
    12601 acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg ctgtttattg
    12661 aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat caattagaag
    12721 aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt aatgggaaat
    12781 ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa aaattactca
    12841 aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc ggatgctatc
    12901 aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata ggattcgatg
    12961 gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag ccaagttttg
    13021 atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct caaagaaaag
    13081 ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa
    13141 agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt tagtgctttt
    13201 tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat aatgtataat
    13261 accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg aaaaaaagag
    13321 ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact atacgaatgg
    13381 aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc aagacatgac
    13441 aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg tcatcgattt
    13501 gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg gtgacacgac
    13561 cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta aaatgtctgg
    13621 acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc ccggatttca
    13681 gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag tagcggtaat
    13741 tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc gtgacgtcac
    13801 agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc
    13861 aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca tgaagaacat
    13921 cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg ctaaccgaga
    13981 aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg gaaatagtct
    14041 agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc tagtagaaat
    14101 gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga caaccgatga
    14161 agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa aattcaaaga
    14221 aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac gaattttgac
    14281 tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc
    14341 aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca aagaagaaga
    14401 tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg
    14461 tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca ttgaattatt
    14521 atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc atgacatcat
    14581 cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa aagaaatgga
    14641 actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag aagctgtgat
    14701 gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca atccagcatg
    14761 ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta cagtaatcgg
    14821 gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag atgccatcaa
    14881 taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat caaaggatga
    14941 tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt
    15001 caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag cttgcgaaat
    15061 agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa caggcggagc
    15121 aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc
    15181 ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg gtgctacaag
    15241 aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag gctattaatt
    15301 ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac agctattgaa
    15361 gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg tctcgtaacg
    15421 gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg attaacgaat
    15481 tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac atttacgcat
    15541 ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg tcgacaagta
    15601 atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa tggtgtgacg
    15661 aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga tctataactc
    15721 atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga ggctttacag
    15781 aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca gggttatttt
    15841 taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc tcatcagaca
    15901 caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca ttcaaccagt
    15961 cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga ggaaatgatg
    16021 gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt gaccaagcat
    16081 tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca gcgatccaag
    16141 aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc atcgatggat
    16201 tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt gcaggtgttg
    16261 cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta aaaggtgaag
    16321 taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg aaagaattag
    16381 gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca gataaaggac
    16441 gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat gcttcaggcg
    16501 gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag
    16561 tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg
    16621 aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta gtagaagatt
    16681 tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt tatggattga
    16741 aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa aaagcagttg
    16801 atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact cctgaagaaa
    16861 actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg gtttgattag
    16921 aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg gaacaactcc
    16981 ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt agtcaatcgt
    17041 tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata
    17101 gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa catcaattta
    17161 caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg caaatctaca
    17221 ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt gatgcgtggg
    17281 aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg gatggtccat
    17341 cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt gggaagcgag
    17401 cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca aaaagtttct
    17461 gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc agttggaatg
    17521 gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact gatttttgat
    17581 gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat catgaaggaa
    17641 ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga aatcaaagca
    17701 gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa
    17761 gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc attcaaaacg
    17821 atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa
    17881 gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta
    17941 cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc tttgactggt
    18001 ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac taataaaaaa
    18061 ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca taaatacggg
    18121 ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca tcaaaaacct
    18181 tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag agaattgatc
    18241 gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt atcaggcgga
    18301 aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt
    18361 gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa acgtctgatc
    18421 gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga agaaatttta
    18481 aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat cgttgatccg
    18541 aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt agaagaagca
    18601 cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa aattccgtaa
    18661 tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga tattgatggc
    18721 tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag cccttggttc
    18781 gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta cagcattagg
    18841 tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac aagcactttg
    18901 cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa aagttgtttt
    18961 gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg ctgttccagg
    19021 tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga tgaactatat
    19081 cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct atcgctcaag
    19141 tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat ggttaacaca
    19201 agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg cgcttatttt
    19261 cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg ttggattgaa
    19321 tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt tgtcgatggt
    19381 catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa
    19441 cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg tttccttgct
    19501 tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta ttttgaagat
    19561 cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca atgtatcgat
    19621 tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt
    19681 cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac caacgaatca
    19741 aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt gatccttact
    19801 tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac tttttctgaa
    19861 cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc ttttagttcc
    19921 atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg gatcgctatt
    19981 ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc aacagtcagt
    20041 ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc tgctttaggt
    20101 gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac gcagaatctg
    20161 gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat tttctttaaa
    20221 aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc
    20281 ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca agctgccgac
    20341 acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc tggcttgtta
    20401 ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc agctggtacg
    20461 atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg gaatccttta
    20521 ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat cattggcgca
    20581 caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc atatttgctg
    20641 acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc aaacgggaag
    20701 aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac gggaagtgtt
    20761 tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa ggaataatca
    20821 tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa gcaccagatg
    20881 ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa gaagcaggat
    20941 tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg
    21001 aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct gtagggaaag
    21061 atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca tttcgtgtgt
    21121 ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct ggccgtaaag
    21181 tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt gaacaccaaa
    21241 tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag atcgcggcac
    21301 atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt cgtgatataa
    21361 caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg ggtgaaccag
    21421 gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc ggacacacag
    21481 tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag atcaacgata
    21541 ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg gatggcgttg
    21601 accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc aatttagtag
    21661 attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag
    21721 aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac ttgctaatga
    21781 ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg cgagaatatg
    21841 taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct caaggatatt
    21901 attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact gaaaatggcg
    21961 aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa tgaccaagtt
    22021 aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa aagaaattga
    22081 ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg aagatgctgt
    22141 aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg ggcatgcagg
    22201 aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag ggagattcca
    22261 ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga tggccggact
    22321 aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat catttgtacc
    22381 aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc ctttgatcgg
    22441 tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt atacgcctga
    22501 ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta aagaaggcgt
    22561 atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa tgtctcggac
    22621 aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag cagctcacag
    22681 cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta tgcaagcgaa
    22741 tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt ttaaagcatt
    22801 ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt gtgcatattg
    22861 aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct ttgcgagcta
    22921 aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta cgcggtatgt
    22981 taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa
    23041 tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta aagaaattga
    23101 ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat ttagtactag
    23161 cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc
    23221 ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa gaaaagggct
    23281 atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat gatttgacag
    23341 agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca gcggctcttt
    23401 attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt agcgaccatt
    23461 tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat gaaatgatcg
    23521 aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct gccctgcatt
    23581 aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg ctggacttat
    23641 tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct gccgtttaac
    23701 tctgagtcac aaggaacaat ttcaaaaa
//
LOCUS       DACCCR010000248        21292 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.047, whole genome shotgun sequence.
ACCESSION   DACCCR010000248 DACCCR010000000
VERSION     DACCCR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.047"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(565..792)
                     /locus_tag="IVK93_002922"
     CDS             complement(565..792)
                     /locus_tag="IVK93_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6388117.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(801..1274)
                     /locus_tag="IVK93_002923"
     CDS             complement(801..1274)
                     /locus_tag="IVK93_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP6388118.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            2504..3070
                     /gene="amaP"
                     /locus_tag="IVK93_002924"
     CDS             2504..3070
                     /gene="amaP"
                     /locus_tag="IVK93_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6388119.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3083..3271
                     /locus_tag="IVK93_002925"
     CDS             3083..3271
                     /locus_tag="IVK93_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6388120.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3284..3841
                     /locus_tag="IVK93_002926"
     CDS             3284..3841
                     /locus_tag="IVK93_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6388121.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3873..4115
                     /locus_tag="IVK93_002927"
     CDS             3873..4115
                     /locus_tag="IVK93_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6388122.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4456..5670
                     /locus_tag="IVK93_002928"
     CDS             4456..5670
                     /locus_tag="IVK93_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP6388123.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5701..5919
                     /locus_tag="IVK93_002929"
                     /pseudo
     CDS             5701..5919
                     /locus_tag="IVK93_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6338..6811
                     /gene="dfrF"
                     /locus_tag="IVK93_002930"
     CDS             6338..6811
                     /gene="dfrF"
                     /locus_tag="IVK93_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP6388124.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            6938..7594
                     /locus_tag="IVK93_002931"
     CDS             6938..7594
                     /locus_tag="IVK93_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP6388125.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            7776..8306
                     /locus_tag="IVK93_002932"
     CDS             7776..8306
                     /locus_tag="IVK93_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6388126.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            8852..9094
                     /locus_tag="IVK93_002933"
     CDS             8852..9094
                     /locus_tag="IVK93_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP6388127.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            9091..9561
                     /locus_tag="IVK93_002934"
     CDS             9091..9561
                     /locus_tag="IVK93_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6388128.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            9689..10903
                     /locus_tag="IVK93_002935"
     CDS             9689..10903
                     /locus_tag="IVK93_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6388129.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(11613..11801)
                     /locus_tag="IVK93_002936"
     CDS             complement(11613..11801)
                     /locus_tag="IVK93_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388130.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            11892..12101
                     /locus_tag="IVK93_002937"
     CDS             11892..12101
                     /locus_tag="IVK93_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP6388131.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            12213..13826
                     /locus_tag="IVK93_002938"
     CDS             12213..13826
                     /locus_tag="IVK93_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP6388132.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            13861..15072
                     /locus_tag="IVK93_002939"
                     /pseudo
     CDS             13861..15072
                     /locus_tag="IVK93_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            15095..16021
                     /gene="glsA"
                     /locus_tag="IVK93_002940"
     CDS             15095..16021
                     /gene="glsA"
                     /locus_tag="IVK93_002940"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP6388133.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            16229..17689
                     /locus_tag="IVK93_002941"
     CDS             16229..17689
                     /locus_tag="IVK93_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388134.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            17910..19673
                     /locus_tag="IVK93_002942"
     CDS             17910..19673
                     /locus_tag="IVK93_002942"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP6388135.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            19757..20215
                     /locus_tag="IVK93_002943"
     CDS             19757..20215
                     /locus_tag="IVK93_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP6388136.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            20324..21007
                     /locus_tag="IVK93_002944"
     CDS             20324..21007
                     /locus_tag="IVK93_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6388137.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            21154..>21292
                     /locus_tag="IVK93_002945"
     CDS             21154..>21292
                     /locus_tag="IVK93_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP6388138.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SY"
BASE COUNT         7395 a         3281 c         4358 g         6258 t
ORIGIN      
        1 ctcgagttac gagtttggaa cagcttttgt ctacagtctg tcattattat caaaataatg
       61 ttaaaaataa aaaagaattg tccatcactg ttattcaaat cattttatca aaactacata
      121 gtaaatgtta aaaagcaaca tgacataata tatagaaggc tttacagtat atatgggagc
      181 ttactaaaaa agaatggagt aagagttaac acaatgggcc attagctgaa gttttaagca
      241 cttcaaggta tgaaacagaa tggcctgtaa tactacatga ttatttagac tgctcaaact
      301 gctatcagtc tggtggaatg aataaagtat tatactaaaa gcagatccaa ctacaacgta
      361 acaggaaacc agttgtatca ggaaaagtga tagtacatgg agaagaaaag gccccctttg
      421 tatgagtcag tacatatctt atttatagat aaaacggtat tcaatcgaaa taaaaaatct
      481 gaataataga cgtaaataac ttctaaaagt ctcacacatc tatcattcag attatttttt
      541 atttactgtt tgttctactt tattttattc ttctggtata gtattcgagt catttttata
      601 ggtcaacaaa tcacctggtt gacactccaa aacttggcag attttttcga gagtggaaaa
      661 acggatggct tttgcttttc cattttttag aatagagatg tttgccatcg taatgcccac
      721 tttctcactc aattcagtca cactcatttt tttttgagct aatactacat ctaaattaac
      781 gataattgcc atgtcgcacc tcacactgtc aaatcatgat ctgtcttaag taaaatcgca
      841 tgttcaagca gtttttccag cacgcttgtt aatgtgtata ctgcaaaagg aatgaatacg
      901 acagcgattc ctaccaacat caaacctgga gcatcgtcta aatccgctac tttcataaat
      961 ttaggaagta agccaaaaaa ggcgaaacca ataaacaaaa tggttcgttc gatttttttt
     1021 agtatgttta agctttgagt ggaaaaagct tggttatctt cgattttttt caatagtttc
     1081 aataagaaat aagcaataac gatccctagg agaagggcaa agtaaatgac agaaacaaat
     1141 aatgtggtct gccaatcaaa attgaatcct ttttcgcttg tagcgatctg agtataaaaa
     1201 agtacgccga acaaaataaa tacggcactt atacctaaaa cgacaaattt taatagcaat
     1261 gttttaaatt tcatttttat ccctctttta tctttgatag ctaaaatata gcagaataat
     1321 tattgtaaaa caactatttt ttatcgaaaa acaataaaaa tatatcgttt ataaaaaatg
     1381 atagagtatg aaggtgtgga gtgcatttaa cagctttttt tgattgtaag aaacatttaa
     1441 aaccagttat tttagattct atgtgtgtct cttctataat gctagtttag atgcctggga
     1501 ttatattgga caatacagtt ggaggatttt ttattatcta gcgaagagaa gtgtaagtta
     1561 acagaactgt atgagaaaaa agacaactac gtagtagcta accgattcat taattttttc
     1621 aagttcatgt ttatttacgt atcagaattt ttcttattca atggtttggt gtaatcacca
     1681 cttatttgtc ctttattgct tatacaatgt tatacaaaag gaaaatcaag cattgacgca
     1741 tggggtggta ctatctagct gaaatagctt aataagcgca agtagatgca ttggattagt
     1801 ttataaatca aaagtgttag aggtattaag aagaagtaga aaaataaaat ggattgccga
     1861 ataaaaagta ccataaatcc taaaacatgt gccaacagat agatggcaca tgtttatttt
     1921 tttcgataat tttgtaacaa aaatacgaag atgaaaaaga atagagattc ccataatgta
     1981 gtagtccaac tgaattcttt actgatacca aataccatat ttcctagatt tgatacaatc
     2041 aaatagccaa tcgttatgat caatgaatag tacaatcttt tcatcatagg acatacctcg
     2101 tttttattta taagaatagt ataaaccaaa gaagtgaata agaaaaatga taatataaaa
     2161 ataaagtaag aacgcattaa agtaactaaa gttatttatt gtatttttta attctccgaa
     2221 aaaatttaat aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt
     2281 aatcttgaat aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa
     2341 gcaattgtta tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg
     2401 gtagcgcttt atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt
     2461 agagttattt acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa
     2521 aataatcggt gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat
     2581 caatgcgccg tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa
     2641 ttactacatg caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat
     2701 catcttgcta gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga
     2761 agatggcaaa ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca
     2821 cgaagaagag ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca
     2881 tatccatgtc aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt
     2941 gatgcaagat atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt
     3001 ggcagtcaca tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg
     3061 agtggaatag gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat
     3121 ctttgggggt cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac
     3181 attgttgatc ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc
     3241 aggaatagtt gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat
     3301 gaatacaaca aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg
     3361 tgaattgact tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt
     3421 agatggtcta ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa
     3481 cacagataac gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga
     3541 tctagatgtg atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga
     3601 attgatcagt actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa
     3661 tgttgcagac atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa
     3721 agtaacagaa gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa
     3781 atctgctgta ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata
     3841 agaagtaaga agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg
     3901 gcggtatcat tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca
     3961 tcgcaaacat cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt
     4021 ggggaccaac aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag
     4081 tattagtcgt ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg
     4141 aaatgaagaa caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt
     4201 tatatgaagt attctttatt ccttatgaaa aaatatcttt aaaaataaaa aaagacgaaa
     4261 ataaacggtt ttctataaaa agaagaccgt ttattttttg tctttatttg tgtgaaaaac
     4321 gaaaaagagc acaacacatc aagcttttat taattgtctg tcttttataa atgcaagtat
     4381 taattcaaaa aaacagaaga aaaaaacaac tacaccttct tttatactcg taatcgtaga
     4441 aaggaggcga aaaaaatgaa tacattacct atcaatacag gagatacagc attcatgatt
     4501 ttatgtacag caatggtttg cctgatgact cctggtctag ctttttttta cggcggatta
     4561 gcacggaaga ataatatact aacgatcatg tctcaatctt ttctctccgt tgggataaca
     4621 acgattatgt gggtatttgg tggattcgga ttggcctttg gacgggatat cggcggtgtt
     4681 attggaaacc cggcagattt ctttttgatg agacatgtta caagcttccc caacatgttt
     4741 catggtgcaa cgattccatt tttaatgttc tttctttacc aactgatgtt tatcgtgatt
     4801 accgttcctt tgatgacagg agcttttgca gggagattga atttaaaagg ctatattata
     4861 cttgttattt tttggaatct gctgatttat ttccctgttt gccactggat atggggtggc
     4921 ggatttttgg atcagatggg atttagagat ttcgctggtg gagcagtcat ccacacgaca
     4981 gctggattcg gctctttagc atgtatcctc acacttggtc aaagaaagat tcctttagga
     5041 gaaagaagtc agcatagcag tcttatggca gctgctatcg gtacaggact gctttggttt
     5101 ggctggttcg gttttaattc cggtggtgca cttcgagcag atgaacaagc agtcaatgca
     5161 tttggttcaa cattcatcgc tcttgcattt gcgatgatta cttggctgat cattgcaaaa
     5221 gtaaaaggaa atggctttga ttttgtcgac gtactaacag gatccgttgc tggattggca
     5281 gccattacgc cttgtgccgg ttatgtagaa gcaaaaagtg ccatgctgat tgggatcatt
     5341 gcgggtatcg tctgtcatgc tgctgttgat tttcgtaaaa agaaacaatg ggatgatgca
     5401 cttgatgtgt ggggtgttca tggcatgggc ggattcacag gtacgattct gatagggatc
     5461 tttgcatcta acagccattt actaacgaat ccaagcagct ggtacttctt agggattcaa
     5521 gtgattggtg tcatcattac tgccgtgtat gcctatgtgc tgacaacagt gattttaaaa
     5581 tcggcgatgc actttacaac gatcactact acaaaagaag aacaagaaga aggattggac
     5641 tttttcttct ttggcaacaa actcaattga aacaaattca aatagaaaag aggaagattc
     5701 atgcaacaaa gcaaaacgaa aattcctgca aaaaaattaa cgttctttgg ctttttggct
     5761 atgaccatct caatggtcgt ttcattatat gaatatccca cattcgcgac ctcaggcttc
     5821 agcttagtat ttttcttgtt attaggcggt ttgctatggt ttatccctgt ggcattgtgc
     5881 gcagcagaga tggcaacggt gaaaggatgg gaaaaaggtg tttcccaaac aaaaagatat
     5941 taagaaacag cccatgttta cctgcttgca aacaaaagaa tattgctact agactaagag
     6001 atactacata agtgtgttgg tatctctttt ttgttgccgt gaaacagcag actatttcat
     6061 ttctgcttga ttaccttaaa acttattagt ctatggtttg tcaagggctt gttgcgtgag
     6121 aaatgctgaa tatcaccctt tacaaaacat aggctgataa gtaacttttc aaaagcagaa
     6181 aagaaatggc aatgcttata acaatatgat ataatgcaga tgaagacaaa aaaggaattt
     6241 gtcgagctgg ttagttcgag ataattttga gtttgttaag atgtggtgca cttgatggaa
     6301 tcacaggtac gacatcatat tatggaggat gaaatagatg ataggtttga ttgttgcgag
     6361 gtcaaagaat aatgttatag gcaagaatgg taatatacca tggaaaataa agggagaaca
     6421 aaagcaattt agagagttaa caacgggtaa tgtggttatt atggggcgaa agtcttatga
     6481 agaaatcggt catccgttgc ctaatagaat gaatattgtt gtttccacca caacagagta
     6541 tcaaggagat aatttagttt cagttaaatc attagaagat gcattattat tggctaaagg
     6601 acgagatgta tacatatctg gtggatatgg actatttaag gaagctttgc aaatagtaga
     6661 taaaatgtat atcacagaag tagatttaaa tattgaagat ggagatacat tctttccaga
     6721 atttgatatc aatgattttg aagttttgat aggggaaaca cttggtgagg aagtgaaata
     6781 tacgagaaca ttttatgtaa ggaaaaaatg aattgagtaa attttggatt tagggtgttt
     6841 tcataaatat attgcttttt atgcatatat aaattgttgt aagacaaatt ccagtttgta
     6901 tggggcgata aaatcagaat ttgagagagg attgaatatg aataaggaat ggtctgaact
     6961 taataaaaca atgcaggcac aaataaaaaa gaaagatact tataagaggg gtattgatac
     7021 tttacttact ttacgaagtc agttaataca aaccttagta tctttcaaag aagaattatg
     7081 cagagaagat tttaactcaa tccccttcat aaacgctgat ggttatcata gtaagacgat
     7141 tgcttattct atttggcata ttttccgcat tgaagatata gttgtgcata cagtgataaa
     7201 tgaagatgaa caagtatttt ttgcaggcaa ttatcaagag cgtatcaatt cacctatcat
     7261 tacaacagga aatgaactca tgaaacagca gattgcagac ttttcaaaac agcttaatct
     7321 ggaagaatta tatttatata ttttcgaagt atgggaaagc actgagaaaa tgcttgaacg
     7381 gttatcttat gatgaattga aaagaaaaat accgaaagaa agaaaaggat acttagaatc
     7441 gttgaatgta gtaaacgaca atgaaaaagc aatttggctg attgattatt ggtgtaataa
     7501 agatatttgt ggactaattc aaatgccgtt ttcaagacat tggattatgc acacagaagc
     7561 ctgtttgcgt ataaagaaca agatacattc ataggcaaaa atttcagttt gtagaactaa
     7621 ggaaattgga atttaaggag gaataaagta tggttagtga ttttataaga gcagcatttc
     7681 catggatttt aatgggttta tttgtagcaa tcagttgttc ccttatgagt aaaaaagaga
     7741 aataaattcc tgtttgtatt tataaaagag aaaaaatgag aaaagtagtt ttatttattg
     7801 ccatgagcct tgacggatat attgcggacg gtaatggtgg agtagcttgg ctaaatggtc
     7861 atggaaatga caatgaaaac atcgacactt atacagaatt taccaaagat atagatactg
     7921 tcttaatggg gtggaatact tctcatcagg ttgttactga actttctccg caagaatggg
     7981 tatataataa atttacaact tatgtattaa cacataaaga gtgcaattct tcccaacaaa
     8041 ttcattttac aagtgaaaat cctgttttat tattggaacg gctaaaacaa gaagcaggaa
     8101 aggatatttg gatttgtggc ggagcaaatc ttgttcagca gttggtaagc aaaaatatga
     8161 ttgataaata ttacatttct gttattccta ctctgttagg aaatggggtt cgtcttttgg
     8221 gcaacataga cagggaaata aagttaaggc tttgtaaaac acagacttat aacggaataa
     8281 ccgatttgat ttatatgcgt agataactag agagaaacag aaaagctgtt aaaccgacgg
     8341 agcgatccga ccacgttggc gggaaaatcc ttaagatttt tccgccatgc ttgcaagggg
     8401 gcggttcgct tcggcggttc ggcgagcttg cgagccgttc aaagcccccg ttatctaaca
     8461 aggggggcac aaaaaacaag aaacactata cactaacaag cggaaaacgc tgtatttata
     8521 aggcaaaacc ccatttttac agtagtgata taaattgtac ctatatcact atgaaaattg
     8581 aatagtaaat atgagaaaga cagttgattt caaaaaataa gaaattgatt gtcgttttct
     8641 tttgcagaaa tttaagtgca ggcaattatg gtagttgtcg taattgctgt cagctcagtg
     8701 tttatcactg atggtgtgaa agatttacgg ttggagatgt tcaataagat tattggtgct
     8761 taattatttt tattgtagtg ctgatatgag aatgttataa ttggggtata aaacatacaa
     8821 ataaatttgc tgaaaaatga ggtatcaaaa tatggaaaat gaattactgg aaaacttagg
     8881 aagacttcac acaactgaac ttggcgttat tagaattaaa aagaacttat ctttgaatgt
     8941 agaaaatgta attgaatggt gcaaagaaaa aattagcctt tctaatgcgg aaatcacaag
     9001 aaaaggaaaa aactggtata taacgattga taattgtatt ataacggtaa atgcgtacag
     9061 ttataccgtt atcactgctc acaaggtaaa atgataaatt tttctataaa ggaattaaac
     9121 gcatttgcag ttattgggca ggaagtagaa ctgactaatt atcaaaaaaa gaatattcag
     9181 attagtacac agttttggag aaagtttaac agtagtttga aaaaagcgta tctttcacaa
     9241 tcaggaaatt gggtaaaata tgcttttatg gaaagaagaa atggaaaact ttattatttc
     9301 tgttctattc cccaaagaac cattacccca gagggctttc tgtataaaga aataccgtct
     9361 tataaatatt tggttgtgga gcatattggt gctatggata aaatatatga aacttatggc
     9421 aagatttatg aagaaattat tcctagtaca tcgtatattc ctataaaaga tattatccta
     9481 cactttgaaa aatacgatta tcgttttcat tggaatagtg ataattcagt tattgaaatt
     9541 tggataccta ttcaagatta gaagcaattt tatatccata cacacaaagc agttagtctt
     9601 aggattgact gctttttcta tacagatttt taatgacagg caattatcaa ttatggtagt
     9661 tgcctttttt gattggagga attgaagaat gaatgataaa aactttattg aagaactaag
     9721 acaaaagcgt gaggaatacg gagtaacaca aacaaggctt gctgttgcct gtggtatcag
     9781 ccgtgaatat tacaaccgca ttgaaaaagg aaaacagcca ttgaatgacg aattaagaga
     9841 aatcatagaa aaacagattg agcgtttcaa tccgcaagaa ccactattct tattgattga
     9901 ttactttcgt gtccgctttc ctactacgga cgcattggcg attatccgtg atgtattaca
     9961 actgaaatct aattacatgc tttatgaaga ttatggaaaa tacggatatg aaagcaaata
    10021 tgtacttggc gacatcaata tcatgtgttc catgcaggag catttaggtg ttttattgga
    10081 actgaaaggc aaaggctgtc ggcaaatgga atgttatctg ctggcacagg aacgctcatg
    10141 gtatgacttc atgctggatt gtatgacggc tggtggtgtg atgaaacggc ttgatttggc
    10201 tatcaatgat aaagcaggaa tattggatat tccaaagtta aaggaaaaat acaaggctgg
    10261 cgaatgtatg tcctacttcc gtatgcagaa agattacagc ggtacagaaa aatgcggtag
    10321 cgatttacca aagaatacag gagaaacctt atatctcggt tcaacaagta gcgaattata
    10381 tatgtgtgct tatcagaaaa attatgagca gtatgtcaag aatggcacag aaattgaaga
    10441 tacggagatt aagaaccgtt ttgaaatacg catgaagaac gaacgagcct attatgcggt
    10501 tgtagattta ctgacctatc gggacgctga acgcaccgct ttttctatca tcaatcatta
    10561 tgtccgcttt gttgacagag aggacgacaa gccaaaaagt caatggataa caaatgatga
    10621 ttgggcatgg tttgtagggg aaaacagaga gccgatacgc ttaaccacaa agccagagcc
    10681 ttacacttta caaaaagcgt tgcattggct acaaagacaa gtcgcaccaa ccataaaaat
    10741 ggtacaggca ttagacagag aaaatcgtac aacgatactg aaagatatga ttgagcaggc
    10801 agaactaaaa gacaagcata aacatttatt acaattagag aaatcaacca tagaagaacg
    10861 tatagatacc gctgttccac aagagaatga cggtattttt taatgtaatt gaaaaatttc
    10921 agatttagtc agcaaagagc tgttttcatt ccctatgtga agtgaaagaa gaaaaataaa
    10981 ttttttatta aatctgcaac aaaatggcta cttgcgtaca gatatggtgc gagaaaagga
    11041 aatctaaact ttttttgaaa ataggtcatt aaatcggggc ttgctgtccc tatatgaagt
    11101 gaaagaagaa aaaagttttg aaaaaatgac ggaaattcgt gattagtgga cagtcttctg
    11161 tgaaaagagg aaaaacatca tcattttgta ttcaaacagg cattagcagt tgagtgttga
    11221 tgtgaaaaga tgaaaatact ttgttctcaa cttaacaatt taaggtttcc gttccctata
    11281 tggtgcaaag aaaatatttc attctattgt tggcagtaac ggtatggttt gggtagttag
    11341 ggtgtgaaaa ggaaaatatt cttttttcat catcaactta gcaaaatgac attttctatc
    11401 cctatatggt gcatgaaaag aagttaaaat ttttccattt aaacttagca aatggacgtt
    11461 tgctgtacag atagtagtga aaaaggagaa aatcaaccgt tgattgccaa aatgaaccgt
    11521 tccatttgta ctaataatgg aaaaagtttt ggattttggt tacgtttctc ctcttttcca
    11581 acgcgatata taggaaagac gatttttttc tttcaatcgt tctttgacaa ctgaataaag
    11641 ctattcaata cgtttgacag acagctagcc gttgaaacaa atacgccacg acctttcccc
    11701 tgcaagagcg agcgaaaacc tatttgtgtt cttgtaaaag atttgtcctc ttttatcgcc
    11761 atgacctgta ctgtcagata atgatactcc cgtacagcca cagcttgagc attaagagcg
    11821 tcgcacgcaa tgggtacggc tacataagaa ctatgcagag gtggaactcc tgtgggctat
    11881 gacaaaagcc gttgaattgc ttagaaagat tttacagaaa gggggtatgt gttattgata
    11941 atgctgtaaa aacaattcaa aagaaaaatg gcaagtgtgg cattggcaac agaggaaaga
    12001 caaagattgt agtaaaagag catttttccg aaaagggcaa aacaatggaa gaacttctaa
    12061 ctgatgtcat gctggaaaaa gcaaagcaga caatcgcata aaatcggtgc agttgtaaga
    12121 aatagattga atgacaaaaa gtacccgtgt tatactttac ttgcaagcag gtattgtaaa
    12181 cacgggttag ttataaagga ggataactga caatgaaaca acagatttac aatactgcac
    12241 tttatcttag gttaagccga gatgatgaat tacagggcga aagttccagc attaccacac
    12301 agagaagtat gttgcgttta tatgcaaaag aacatcattt gaatgtcatt gatgaatata
    12361 ttgatgacgg ctggtcggga acaaattttg acagaccgag ttttcaaaga atgattgagg
    12421 atatagaggc aggaaaaatc aactgtgttg taacgaaaga cctttcccgt cttggcagaa
    12481 attatattat gacaggacaa tatacagaat tgtatttccc tagtcataat gtccgctaca
    12541 tagcgattga tgacggtgta gacagcgaaa aaggcgaaag tgagattgca ccatttaaga
    12601 acatcatcaa tgaatgggtg gcaagagata caagccgtaa agtcaaatca gcatttaaga
    12661 caaagtttgc ggagggggct cattacggtg cttatgctcc gttaggatat aagaaacacc
    12721 ctgacatcaa aggaaaactg ttggttgatg atgaaacaaa atggattatt gaaaaaatct
    12781 tttccctagc ctatcaaggt tacggaagtg caaaaatcac aaagcagtta agagcagaaa
    12841 aagttccgac agcgtcatgg ctaaatttta caagatacgg tacttttgca catatctttg
    12901 agggaaaacc cgaaagtaag cgttatgagt ggacgattgc tcatgtcaag gcgatattga
    12961 aaagtgaggt ttatatcgga aacagtgttc acaatatgca gtctacggta tcgttcaaga
    13021 gcaaaaagaa agtgcgtaag cccgaaagtg aatggtttcg agtagaaaac actcacgagc
    13081 cgattattga caaggaagtg ttctatcgtg tgcaggagca gataaaatca agacgcagac
    13141 agacaaagga aaaggcaacg ccgatttttg cagggcttgt caagtgtgcg gattgtggtt
    13201 ggtctatgag atttggaacg aataagacaa ataaaacgcc ttacagttat tatgcttgca
    13261 gttactacgg gcagtttggc aaaggtaatt gttctatgca ctacattcgt tatgatgtgc
    13321 tgtatcaagc cgtattggaa cgcttgcagt attgggctaa ggcagtacag caggacgaag
    13381 aaaaggtatt gaacaagata cagaaagctg gcaatgcaga gagaatacgg gaaaagaaga
    13441 aaaaggcaag tacattgaag aaagccgaga acagacaaaa tgagattgac cgtttatttg
    13501 cgaaaatgta tgaagataga gcctgtgaga agataacaga gcgaaatttt gtgatgttgt
    13561 ccagcaagta ccaaaaagaa cagatagaac tggaacagca gataacaagc ctaagagaag
    13621 aactaagtaa aatggaacag gatatgatag gtgctgaaaa gtggattgag ttaatcaagg
    13681 aatattccgt accaaaggaa ctgacagcac cattattaaa tgccatgata gaaaaaatcc
    13741 tcatacatga agcaacaacg aatgaggata acgaaagaat acaggaaatt gagatatatt
    13801 accgatttat tggaagagtt gagtaatcaa caagagtaat atttttaact aagggaaacc
    13861 ggcggtgtct atacttgggt cagtcgcaca ttgggaaaac gattcggttt tgccgcaatc
    13921 tttttccaat ggtttgagat aacagttggc tacttgacca tgctgtattt cttaactggt
    13981 gcattatcct atgcaacggg tattggtgct atacaaaaca ataaatatct gaaattggca
    14041 atcctattga ttattttctg ggctatttta ctctcacagt taaaaggaac caaatacact
    14101 tcattgattg ctcggatcgg ttttgtagct ggtatcttgt tgccagcatt agtcttgttt
    14161 ggcttaggga tccattatgt cgcttcaggc gctccactac aaacgatact gtctatgaaa
    14221 acattgattc ctgatttttc gaaattgcct acgctggttg tttttgtttc ctttatttta
    14281 gcatatatgg gcgtagaaac atctgccagt catgcaaatg aaatggaaaa ccctaaaaag
    14341 gattatccat tagcaatgtt tgtattggtt attctggcaa tcattttaga tacttttggc
    14401 ggattgaccg tcgctactac agtacctcaa catggtttgt ctcttaatac aggggtgatt
    14461 caatcgttag aattcctatt aagacacatt gatccaaatc ttgcatggat tgcgaaaatt
    14521 cttgcgattc ttgtttgttt aggtgtcatc ggagaaattg cttcttgggt aacctctcct
    14581 tcaaaagcac ttcacgtagc agcagaagaa ggattacttc ctgaatattt tgcaaaagaa
    14641 aacacacacg gtgttcctgc tcatttgatg atcgcaaatg ggatcgttgc aacaatctgg
    14701 gcagccgtct ttacattaag cggcgggggg aataatcttt catttcttgc agcgatgtca
    14761 ttgactgttg taatctacct gatgttgtac tttatgtttt tcgcgggcta tttgaagctg
    14821 gtttttaaag atgacagtct tgaaagagcc tatcaggttc caggcggaaa agttgtaaaa
    14881 gtaatcgttg cttgtttagg tcttttgact tcactttttg ctttttgtat ctcatttgtt
    14941 ccacctgcta gtatcgcagc tggacagcat gggatttatg aaacgattct gattgtttct
    15001 tatcttatcg tcttagttat tccatttacg atctacgcat gccgtcataa attccagcat
    15061 gcagaaaaat aaaaaatgaa agctggttat tcaaatggaa gtaaaagaat tagaacaatt
    15121 gattggaaaa aatttccact attatcaaac aggacaagtg gcggattaca ttccagcatt
    15181 agctgaagtg gatcccaaac aattaggtat ggcgatttat gatttggata caaaacaact
    15241 gattgaggga ggagacgccg atgtgcgctt tgccatcgaa agcatgtcaa aagttcccgt
    15301 attgttatta gcaatcaaag acaacggaat agaaaaagtc tttcaaacaa tcaacacgga
    15361 accaaccggt tttgccttca attctatcat gaatatggaa atcaatcatc gcaaacatcc
    15421 aatgaatcct tttgttaatg caggagcgat tgcaacaacc tcgttgattg ccggtaaaga
    15481 tgcagatgaa aaatttgagc gaatcttgtc atttatgaaa gagatctgtg atgatcctaa
    15541 tatcacattg aatgaagaaa tctatcattc tgaatcaaga acaggtgata tcaatcgctc
    15601 tcttgcttac tatatgaaag gtaatcaaat gatcgaagga gacgtaccgg agatactcga
    15661 tgcttatttc agacaatgct ccctcaatgt caccgctaca ggcatagcaa aattagctgc
    15721 tgtgctggca aataaaggaa ttgctccttg gaatggcaaa caaatcatta ctgaagaaag
    15781 tgctacaatc gtcaaatcaa ttatgacaac tgctggtttg tatgatgaat caggagagtt
    15841 ttccgtccat gttggtgtac ctgcaaaaag cggtgtaggc ggcgggttga tggcagctgt
    15901 gccaaatcgt tacggcatag gtgtcttcag cccggcacta gatccatttg gtaacagcgc
    15961 agctggtatc caattgttga aagatgtagt caaagaacta gacgctgaca tctttgaata
    16021 accattcttg aaagaggctg gggtaaaagt ccaaacggct caaagctacc tacaatgagg
    16081 ctgaaattgt gagcttgttt gacttttgtc ccagtttctt tttttattgg tttttgaaag
    16141 ggcttttcta aataaaaagg aaacaaaaat acgatttaca gatatgatta aataaaatca
    16201 taagaaaaac tggaggcgtg tatcaggaat gctggaaaat tacatcgagc gaaatatatt
    16261 tagaaaagtg tatctgtgtg aacaattatt tgaattccaa gaaatagaca tcgaacaaac
    16321 agctatttct ctaagggtga ctacaccaac gatccttcac gatctagaaa gtctagccga
    16381 gtgtctagaa tattgtataa aagaacaagt aagagagaag cataagtata aactcgtatt
    16441 caaacatggt attgctcttt ctgagctcac acagtttctg tacggtcaat cttatttttt
    16501 aaagttttta agttattatt ttaatggtat attcacgagt acagaactgg ctgatcttga
    16561 atttatttct ttgagtaaag tatatacaat caaaaaaatc gtattggatt tttttaaaga
    16621 aaatgattat ttgaaaaata aagaaatcat tatcccagaa tttgactcgc ggaacatctt
    16681 gctagcttta gtacgttatg tcaattggga aggctatgaa aataaaaatc cgtatgtaga
    16741 agaagcttgt aacgagttga ttgattatgt agagactcat ttttttaaaa gaagatattc
    16801 ggaagatgaa aagtccttta tcatacgaga aatagagatc gcaatcggaa gaaaggcatt
    16861 tccccttgga ctgaaagaag aagataagaa aagcgcagaa acgaagccgt tatttcaatt
    16921 agtctgtcaa ggattggaag agcaaaaagg aattctagac ttacaagaag aagatgtcta
    16981 ttatattttt tctttgttca acagtagaaa ttataccaat gagaacatgg agctgttaag
    17041 aaaagatttc gaagttgttt ataaaaattt tgttctcaat gacctatcac tgaatgaatt
    17101 gattttacag attattagta tcgctgaaat cgacgatgca gaaaatttta ttttcaagca
    17161 atccttttta caatttgtcc gtacgttgtg ggcagatgga caagtatttt taccggaaag
    17221 gatctatctt ctatcagaaa aagaacaact tctgtacgga aaaattgtag aaatattgga
    17281 agagtggaaa aagaaaaatc aaatccaact aagatggaat gaaaatttca ttcgaaaatt
    17341 cgtcaaaagt ctttctttga tcaacagtga acaagcgggg agacaggaaa tagaaatttt
    17401 tgttgtttca gaaagttcag tcaaacaatt tttttatcga acccaatttc atttgtatgc
    17461 ggaagaaggc atcgctgaaa taaatcctat tttatttcgt tcattagaag aattgcctga
    17521 tgaatgtcta tgtgcgaaaa aaagagtggt tttgtgcgat gctgctagct atcaaaatgg
    17581 attagaaact gaaaagacaa agatctatcc aatctcatta aaaggtgcaa acatccattt
    17641 gtatcatatt gctcaggaac ttcacctatt aaaaaaacat atttcttgat gagaccttaa
    17701 ttaggagacc ttctacaaag ctttttaatt gtctgtagaa ggtttttatt tgagtcctga
    17761 ttcgtgcgaa gatctactta tataacaagc tttctctgaa atttttttaa aatgctattg
    17821 acttagagtc aactctaagt agtagaatag ataaagtgta acaaatctat tttcattaaa
    17881 agaagatagt acggaggaga gaatgtatta tgagtacagt attacatgta ttggatgcaa
    17941 tcaatagccc cgcagatgtc aaaaaattat ctgttgagga aatgaaacag ttggcagaag
    18001 agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg tgggcacgtt ggtccgaatc
    18061 taggtgtagt agaattaacg attgcgtttc attatgtatt tgattcacca aaggataaaa
    18121 tcgtttggga tgtttcccat cagtcttatg gtcataagct tttaactggt cgaaaaaatg
    18181 gttttgaaaa aggacattac cgtgaagtga gcggatattc caaccaacat gaaagtaaac
    18241 atgactattt tacagtgggg cacacatcta catctatcag cttagcaaca ggtttagcta
    18301 aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc atttatcggt gacggctccc
    18361 taagtggtgg cttagctttt gaaggattga acaatggtgg tgttttacaa tccaatctaa
    18421 tcgtattagt aaacgataat gagatgtcta ttgatgagaa tcatggcagt ttatataaaa
    18481 acttagctga gcttcgtcaa accaacggta aaagcagcaa taacctattt aaagcaatgg
    18541 gatttgatta tcgttacgtg gataatggaa acgatctgga aacggtgatc cgtttatttg
    18601 aagaagtaaa agatattgat catccgattg tacttcacgt ccatacggaa aaaggtcgcg
    18661 gttatcagcc ggcaatcgat aataaaatga aatatcattg gcatgtacca tttgatctgt
    18721 ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt taatatgatc atcatggatt
    18781 atttggataa gaaaatgcaa acaagtacag ctcctattgc cgcagtcaat gcagcgatac
    18841 caggcgcctt tggactgaaa acattccaag aaaaacatcc agatcattat tttgacgtgg
    18901 ggatcgctga acagcactct atcactttcg cttctgcttt ggcttctcag ggagtacgtc
    18961 cagtcgtctt ccaatcaagt actttcttgc aacgggcata tgatcaatta tcacatgatt
    19021 tagcgatcaa cgagaatcca gtgattattt tagtcggtgg cggaacgatc tcagctagtg
    19081 accgaacaca ccaaggaaca tttgacattc cttacttatc aagtatcccg aacatcaaat
    19141 atcttgctcc agcaacaaaa gaggatatga tctctatgct agattgggcg ttcgaacaaa
    19201 cagcgcagcc tgtagcaatc cgaattccta cacatggtgt agaacatggt acagctatcg
    19261 caaatgatta tagtattcca gcctatcaaa cagtaaataa tggaggaaaa gtagcaattt
    19321 tagctttagg cggttttctt gaattaggaa aagaaacagt aaaagctttt agaaaaaaag
    19381 gaatcgaagc aacgttgatc aatccaggaa cgatcacaga aaatgacgta gaaacattag
    19441 ataaattaaa agaaacacac gaattagtca tcacattaga agatggtagt ttgtcaggag
    19501 gatttggcga aaatatcagt cgttattatg gtgattcttc aatgaacgta ttaaattttg
    19561 gtgcagagaa agaattcacg gatagcgttc ctgtagggca gctttatgaa cgttatcatt
    19621 taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt gaatattcaa taattcatta
    19681 tgctcaccat ccagataatt gtttatacta gtatctggat ggttttttca ttataaaaag
    19741 caaaggggtg gatgttatgt atcagcaagt agaagaatat gtcaaaaagt tccaagaaaa
    19801 atatcctagt tatcaatcag aaaaattagg tttcttttca tttggggata cagaaaaaat
    19861 ggcagacgag ttgagcgaac tagttgtaaa aaagataaag actggtacat caagtggtta
    19921 cgatctgtat aaacaagaga atgagccttt accaaaagtt ggtcaattag acgtggtcct
    19981 gaatggaaaa aatgaaccag tttgtatcat caaaaatata aatgtgacca tcttgccttt
    20041 tagtgaagtc acgaaagagc atgcttggaa agaaggagaa ggggatcgga cattagctta
    20101 ttggcggaaa gtccatgctg agttttttaa acatgcttat aaagaagcgt tgaacataga
    20161 atttacagaa gagaagctgg tcgtatacga agaatttgaa gtaattttct tttagtaaga
    20221 aaggtctgct gaaagaaata gctcattatt gtctcctgaa tagaaaaggg ttacattttt
    20281 atatgaagct gtatttttat ataacattac tggaggagat attgtggata cactggaaca
    20341 aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa aacgagccgc tacacagttc
    20401 ttttattatg gggagaatgg ctttggctgc aaaagaaggc ggagcagttg gtattcgagc
    20461 aaatacgaca gaagatatca atgaaatcaa gcgaatcgtt gatttaccgg taatagggat
    20521 tatcaaaaga gattacgatg ggtcagaagt gtttattact gcgacagaaa aagaagtgga
    20581 agaacttttg gagacgcaag cagaaatcat tgcactggat gcaacttctc gtaaacgtcc
    20641 gacagaaaaa acaactgctg aacttgtcca aatgattcac gaacacggtc ggttagctat
    20701 ggcagatatt tcgacttttg aggaagcgct tcaagcgcaa gaagatggtt ttgatctttt
    20761 gtctacgact ttagcaggct atacagaata ttcacgaaag acgcagacac ctgattttga
    20821 tttattgaat caaatcatag aacaagtcga tatcccagta attatggaag gacatactga
    20881 ctctcctgaa caagtagaga aagcatatga attaggtgct ttttcagtcg tagttggatc
    20941 tattatcacc cgtccgcaat tgatcactaa aagatatgtc gaagccgcaa gtaaagcaaa
    21001 taactaaaaa acacgcacgg cacagattct tgacaaaaaa gaaaaattga tagatagttt
    21061 aaagcagatt ctaagaagat gaaaaaagca ttacttttca ttttgtcttc aagatctgct
    21121 ttttaacgaa ttttatagaa agaaggcgaa accatgtttg aacggagtcc ttcagaattg
    21181 acagaacgtg aaaattacaa gttgctgatt ggttcagtca tccctagacc agtagcagta
    21241 gtaacgacta aatcagcaaa agatatagtg aatatcgcac ccttcagtta ct
//
LOCUS       DACCCR010000249        21056 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.048, whole genome shotgun sequence.
ACCESSION   DACCCR010000249 DACCCR010000000
VERSION     DACCCR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.048"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IVK93_002946"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IVK93_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6388139.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IVK93_002947"
     CDS             2039..2227
                     /locus_tag="IVK93_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6388140.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="IVK93_002948"
     CDS             complement(2285..2638)
                     /locus_tag="IVK93_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6388141.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="IVK93_002949"
     CDS             3169..3591
                     /locus_tag="IVK93_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6388142.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="IVK93_002950"
     CDS             3588..3818
                     /locus_tag="IVK93_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6388143.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="IVK93_002951"
     CDS             4316..4516
                     /locus_tag="IVK93_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6388144.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..5736
                     /locus_tag="IVK93_002952"
     CDS             4543..5736
                     /locus_tag="IVK93_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6388145.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="IVK93_002953"
     CDS             complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="IVK93_002953"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6388146.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7428..8351)
                     /locus_tag="IVK93_002954"
     CDS             complement(7428..8351)
                     /locus_tag="IVK93_002954"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP6388147.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8547..9152
                     /locus_tag="IVK93_002955"
     CDS             8547..9152
                     /locus_tag="IVK93_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6388148.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            9460..10476
                     /locus_tag="IVK93_002956"
     CDS             9460..10476
                     /locus_tag="IVK93_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6388149.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            10617..11918
                     /locus_tag="IVK93_002957"
     CDS             10617..11918
                     /locus_tag="IVK93_002957"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP6388150.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11964..12626
                     /gene="deoC"
                     /locus_tag="IVK93_002958"
     CDS             11964..12626
                     /gene="deoC"
                     /locus_tag="IVK93_002958"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP6388151.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12647..13045
                     /locus_tag="IVK93_002959"
     CDS             12647..13045
                     /locus_tag="IVK93_002959"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP6388152.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            13111..14193
                     /locus_tag="IVK93_002960"
     CDS             13111..14193
                     /locus_tag="IVK93_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6388153.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            14405..15967
                     /locus_tag="IVK93_002961"
     CDS             14405..15967
                     /locus_tag="IVK93_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6388154.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15960..17093
                     /locus_tag="IVK93_002962"
     CDS             15960..17093
                     /locus_tag="IVK93_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6388155.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            17093..18049
                     /locus_tag="IVK93_002963"
     CDS             17093..18049
                     /locus_tag="IVK93_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6388156.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            18148..19314
                     /gene="deoB"
                     /locus_tag="IVK93_002964"
     CDS             18148..19314
                     /gene="deoB"
                     /locus_tag="IVK93_002964"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP6388157.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            19338..20156
                     /locus_tag="IVK93_002965"
     CDS             19338..20156
                     /locus_tag="IVK93_002965"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6388158.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            20173..20880
                     /gene="deoD"
                     /locus_tag="IVK93_002966"
     CDS             20173..20880
                     /gene="deoD"
                     /locus_tag="IVK93_002966"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6388159.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6778 a         3760 c         4523 g         5995 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5341 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5401 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5461 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5521 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5581 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5641 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5701 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5761 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5821 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5881 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5941 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     6001 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6061 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6121 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
     6181 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
     6241 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
     6301 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
     6361 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
     6421 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
     6481 cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga
     6541 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
     6601 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
     6661 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
     6721 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
     6781 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
     6841 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
     6901 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
     6961 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
     7021 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
     7081 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
     7141 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
     7201 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
     7261 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
     7321 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
     7381 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
     7441 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
     7501 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
     7561 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
     7621 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
     7681 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
     7741 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
     7801 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
     7861 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
     7921 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
     7981 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
     8041 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
     8101 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
     8161 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
     8221 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
     8281 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
     8341 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
     8401 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
     8461 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
     8521 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
     8581 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
     8641 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
     8701 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
     8761 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
     8821 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
     8881 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
     8941 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
     9001 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
     9061 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
     9121 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
     9181 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
     9241 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
     9301 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
     9361 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
     9421 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
     9481 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
     9541 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
     9601 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
     9661 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
     9721 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
     9781 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
     9841 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
     9901 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
     9961 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
    10021 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    10081 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    10141 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    10201 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    10261 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    10321 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    10381 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    10441 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    10501 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    10561 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    10621 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    10681 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    10741 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    10801 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    10861 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    10921 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    10981 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    11041 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    11101 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    11161 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    11221 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    11281 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    11341 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    11401 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    11461 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    11521 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    11581 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    11641 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    11701 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    11761 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    11821 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    11881 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    11941 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    12001 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    12061 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    12121 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    12181 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    12241 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    12301 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    12361 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    12421 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    12481 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    12541 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    12601 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    12661 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    12721 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    12781 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    12841 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    12901 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    12961 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    13021 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    13081 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    13141 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    13201 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    13261 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    13321 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    13381 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    13441 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    13501 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    13561 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    13621 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    13681 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    13741 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    13801 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    13861 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    13921 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    13981 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    14041 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    14101 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    14161 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    14221 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    14281 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    14341 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    14401 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    14461 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    14521 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    14581 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    14641 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    14701 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    14761 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    14821 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    14881 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    14941 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    15001 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    15061 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    15121 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    15181 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    15241 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    15301 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    15361 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    15421 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    15481 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    15541 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    15601 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    15661 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    15721 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    15781 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    15841 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    15901 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    15961 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    16021 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    16081 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    16141 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    16201 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    16261 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    16321 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    16381 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    16441 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    16501 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    16561 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    16621 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    16681 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    16741 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    16801 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    16861 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    16921 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    16981 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    17041 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    17101 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    17161 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    17221 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    17281 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    17341 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    17401 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    17461 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    17521 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    17581 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    17641 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    17701 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    17761 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    17821 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    17881 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    17941 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    18001 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    18061 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    18121 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    18181 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    18241 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    18301 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    18361 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    18421 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    18481 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    18541 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    18601 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    18661 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    18721 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    18781 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    18841 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    18901 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    18961 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    19021 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    19081 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    19141 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    19201 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    19261 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    19321 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    19381 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    19441 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    19501 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    19561 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    19621 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    19681 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    19741 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    19801 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    19861 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    19921 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    19981 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    20041 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    20101 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    20161 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    20221 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    20281 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    20341 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    20401 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    20461 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    20521 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    20581 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    20641 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    20701 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    20761 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    20821 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    20881 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    20941 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    21001 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaa
//
LOCUS       DACCCR010000250        21056 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.049, whole genome shotgun sequence.
ACCESSION   DACCCR010000250 DACCCR010000000
VERSION     DACCCR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.049"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IVK93_002967"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IVK93_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6388160.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IVK93_002968"
     CDS             2039..2227
                     /locus_tag="IVK93_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6388161.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="IVK93_002969"
     CDS             complement(2285..2638)
                     /locus_tag="IVK93_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6388162.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="IVK93_002970"
     CDS             3169..3591
                     /locus_tag="IVK93_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6388163.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="IVK93_002971"
     CDS             3588..3818
                     /locus_tag="IVK93_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6388164.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="IVK93_002972"
     CDS             4316..4516
                     /locus_tag="IVK93_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6388165.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..5736
                     /locus_tag="IVK93_002973"
     CDS             4543..5736
                     /locus_tag="IVK93_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6388166.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="IVK93_002974"
     CDS             complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="IVK93_002974"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6388167.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7428..8351)
                     /locus_tag="IVK93_002975"
     CDS             complement(7428..8351)
                     /locus_tag="IVK93_002975"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP6388168.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8547..9152
                     /locus_tag="IVK93_002976"
     CDS             8547..9152
                     /locus_tag="IVK93_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6388169.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            9460..10476
                     /locus_tag="IVK93_002977"
     CDS             9460..10476
                     /locus_tag="IVK93_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6388170.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            10617..11918
                     /locus_tag="IVK93_002978"
     CDS             10617..11918
                     /locus_tag="IVK93_002978"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP6388171.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11964..12626
                     /gene="deoC"
                     /locus_tag="IVK93_002979"
     CDS             11964..12626
                     /gene="deoC"
                     /locus_tag="IVK93_002979"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP6388172.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12647..13045
                     /locus_tag="IVK93_002980"
     CDS             12647..13045
                     /locus_tag="IVK93_002980"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP6388173.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            13111..14193
                     /locus_tag="IVK93_002981"
     CDS             13111..14193
                     /locus_tag="IVK93_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6388174.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            14405..15967
                     /locus_tag="IVK93_002982"
     CDS             14405..15967
                     /locus_tag="IVK93_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6388175.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15960..17093
                     /locus_tag="IVK93_002983"
     CDS             15960..17093
                     /locus_tag="IVK93_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6388176.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            17093..18049
                     /locus_tag="IVK93_002984"
     CDS             17093..18049
                     /locus_tag="IVK93_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6388177.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            18148..19314
                     /gene="deoB"
                     /locus_tag="IVK93_002985"
     CDS             18148..19314
                     /gene="deoB"
                     /locus_tag="IVK93_002985"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP6388178.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            19338..20156
                     /locus_tag="IVK93_002986"
     CDS             19338..20156
                     /locus_tag="IVK93_002986"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6388179.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            20173..20880
                     /gene="deoD"
                     /locus_tag="IVK93_002987"
     CDS             20173..20880
                     /gene="deoD"
                     /locus_tag="IVK93_002987"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6388180.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6777 a         3760 c         4524 g         5995 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5341 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5401 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5461 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5521 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5581 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5641 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5701 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5761 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5821 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5881 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5941 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     6001 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6061 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6121 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
     6181 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
     6241 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
     6301 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
     6361 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
     6421 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
     6481 cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga
     6541 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
     6601 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
     6661 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
     6721 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
     6781 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
     6841 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
     6901 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
     6961 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
     7021 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
     7081 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
     7141 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
     7201 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
     7261 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
     7321 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
     7381 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
     7441 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
     7501 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
     7561 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
     7621 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
     7681 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
     7741 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
     7801 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
     7861 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
     7921 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
     7981 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
     8041 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
     8101 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
     8161 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
     8221 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
     8281 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
     8341 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
     8401 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
     8461 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
     8521 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
     8581 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
     8641 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
     8701 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
     8761 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
     8821 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
     8881 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
     8941 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
     9001 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
     9061 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
     9121 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
     9181 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
     9241 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
     9301 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
     9361 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
     9421 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
     9481 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
     9541 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
     9601 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
     9661 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
     9721 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
     9781 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
     9841 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
     9901 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
     9961 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
    10021 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    10081 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    10141 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    10201 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    10261 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    10321 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    10381 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    10441 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    10501 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    10561 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    10621 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    10681 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    10741 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    10801 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    10861 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    10921 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    10981 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    11041 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    11101 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    11161 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    11221 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    11281 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    11341 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    11401 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    11461 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    11521 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    11581 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    11641 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    11701 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    11761 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    11821 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    11881 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    11941 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    12001 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    12061 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    12121 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    12181 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    12241 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    12301 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    12361 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    12421 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    12481 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    12541 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    12601 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    12661 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    12721 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    12781 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    12841 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    12901 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    12961 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    13021 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    13081 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    13141 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    13201 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    13261 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    13321 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    13381 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    13441 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    13501 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    13561 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    13621 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    13681 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    13741 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    13801 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    13861 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    13921 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    13981 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    14041 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    14101 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    14161 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    14221 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    14281 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    14341 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    14401 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    14461 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    14521 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    14581 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    14641 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    14701 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    14761 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    14821 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    14881 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    14941 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    15001 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    15061 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    15121 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    15181 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    15241 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    15301 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    15361 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    15421 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    15481 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    15541 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    15601 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    15661 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    15721 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    15781 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    15841 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    15901 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    15961 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    16021 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    16081 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    16141 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    16201 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    16261 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    16321 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    16381 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    16441 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    16501 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    16561 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    16621 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    16681 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    16741 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    16801 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    16861 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    16921 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    16981 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    17041 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    17101 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    17161 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    17221 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    17281 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    17341 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    17401 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    17461 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    17521 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    17581 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    17641 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    17701 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    17761 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    17821 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    17881 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    17941 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    18001 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    18061 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    18121 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    18181 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    18241 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    18301 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    18361 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    18421 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    18481 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    18541 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    18601 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    18661 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    18721 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    18781 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    18841 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    18901 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    18961 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    19021 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    19081 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    19141 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    19201 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    19261 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    19321 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    19381 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    19441 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    19501 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    19561 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    19621 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    19681 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    19741 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    19801 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    19861 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    19921 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    19981 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    20041 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    20101 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    20161 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    20221 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    20281 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    20341 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    20401 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    20461 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    20521 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    20581 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    20641 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    20701 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    20761 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    20821 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    20881 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    20941 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    21001 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaa
//
LOCUS       DACCCR010000251        20984 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.050, whole genome shotgun sequence.
ACCESSION   DACCCR010000251 DACCCR010000000
VERSION     DACCCR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.050"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(134..577)
                     /locus_tag="IVK93_002988"
     CDS             complement(134..577)
                     /locus_tag="IVK93_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP6388181.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IVK93_002989"
     CDS             complement(795..2135)
                     /locus_tag="IVK93_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6388182.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVIVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IVK93_002990"
     CDS             2404..3300
                     /locus_tag="IVK93_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP6388183.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IVK93_002991"
     CDS             complement(3450..4391)
                     /locus_tag="IVK93_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6388184.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYNPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4516..5637
                     /locus_tag="IVK93_002992"
     CDS             4516..5637
                     /locus_tag="IVK93_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6388185.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5895..7397
                     /gene="lsa"
                     /locus_tag="IVK93_002993"
     CDS             5895..7397
                     /gene="lsa"
                     /locus_tag="IVK93_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6388186.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7464..7991
                     /locus_tag="IVK93_002994"
     CDS             7464..7991
                     /locus_tag="IVK93_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6388187.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8202..9263
                     /locus_tag="IVK93_002995"
     CDS             8202..9263
                     /locus_tag="IVK93_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6388188.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9266..10162
                     /gene="murQ"
                     /locus_tag="IVK93_002996"
     CDS             9266..10162
                     /gene="murQ"
                     /locus_tag="IVK93_002996"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP6388189.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10176..12158
                     /locus_tag="IVK93_002997"
     CDS             10176..12158
                     /locus_tag="IVK93_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6388190.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12223..12669)
                     /gene="nrdI"
                     /locus_tag="IVK93_002998"
     CDS             complement(12223..12669)
                     /gene="nrdI"
                     /locus_tag="IVK93_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6388191.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12880..14304
                     /locus_tag="IVK93_002999"
     CDS             12880..14304
                     /locus_tag="IVK93_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6388192.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14485..15258)
                     /locus_tag="IVK93_003000"
     CDS             complement(14485..15258)
                     /locus_tag="IVK93_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6388193.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15255..16160)
                     /locus_tag="IVK93_003001"
     CDS             complement(15255..16160)
                     /locus_tag="IVK93_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6388194.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16314..16634)
                     /locus_tag="IVK93_003002"
     CDS             complement(16314..16634)
                     /locus_tag="IVK93_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP6388195.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17108..17794
                     /locus_tag="IVK93_003003"
     CDS             17108..17794
                     /locus_tag="IVK93_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP6388196.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17796..18740
                     /locus_tag="IVK93_003004"
     CDS             17796..18740
                     /locus_tag="IVK93_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP6388197.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18939..19583
                     /gene="pcp"
                     /locus_tag="IVK93_003005"
     CDS             18939..19583
                     /gene="pcp"
                     /locus_tag="IVK93_003005"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP6388198.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19662..20939
                     /locus_tag="IVK93_003006"
     CDS             19662..20939
                     /locus_tag="IVK93_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388199.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6771 a         3780 c         4366 g         6067 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggtgttccag
       61 aagtagcttc ttcttatttc ttgaagctga tcacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg gatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgt caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gttccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaataacaa aattgacgat atccgctgca aatgggatat tcattttttc aaatacaatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat ggctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgattagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccgccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa gcgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttaat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat ggatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttgcttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ctatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc ttttaagcca
     3181 atcccatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaataaataa
     3301 aaaacctaca ttttgaagag attatgtacc gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttc attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat tataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc taatatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gttttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cattatggct atagatcgga tttaattttg cttcttgaaa gacttccaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacgaaa catatcatct
     4141 aattgattcg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtgt gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc tccagctatt
     4381 gcgattttca tgttttttct ctcctttatc aatgagttcc cttttagtat atagaaaaat
     4441 ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta cttaagaaga
     4501 aagtgaggga tacagatgga tattttggcg gcaattgatt catttaaagg aagtgcttct
     4561 tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg aagaaaaatc
     4621 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcggg
     4681 ggaacatggc gtaccattga tgggatggat cttttattcc gtcagcgaaa aatcagctat
     4741 ttattgacaa cttggcaagg aaaaaatacc gcagtcatcg aatctgcaca agcattgggg
     4801 ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag ttatgcctta
     4861 ggagaactta tcaaagatgc actgcttcaa gaagtagaac aaattattgt cactttagga
     4921 ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc ttctttagaa
     4981 gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga tcgaagaaac
     5041 gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc atatacaggt
     5101 caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac gacgctagct
     5161 ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg ggaggtggat
     5221 ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct ttttttgttg
     5281 ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt ggaaaaaaag
     5341 atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca aacgatccac
     5401 ggaaaagttc cacacggtgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct
     5461 atttgcggaa gtagagacca agagctagaa ggactctctg attttctccc aattgtctta
     5521 agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca aacattagaa
     5581 aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc gaagtagttg
     5641 acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
     5701 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
     5761 ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt
     5821 gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
     5881 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
     5941 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
     6001 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
     6061 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
     6121 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
     6181 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
     6241 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
     6301 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
     6361 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
     6421 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
     6481 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
     6541 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
     6601 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
     6661 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
     6721 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
     6781 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
     6841 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     6901 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     6961 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatcc cagccaaaac
     7021 atctgagcat tagttatgta cgccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     7081 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     7141 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
     7201 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
     7261 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     7321 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     7381 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
     7441 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     7501 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     7561 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     7621 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     7681 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     7741 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
     7801 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
     7861 aagaaaatgt ctatctgtta gtttgtcata atgccttgat aagagtgatc aatgcttatt
     7921 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
     7981 cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
     8041 ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc aacctttaga
     8101 aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga tatatactct
     8161 agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat
     8221 ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg
     8281 gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa
     8341 agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata
     8401 tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg cctttgaaag
     8461 caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag attgccgact
     8521 ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg
     8581 aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat tattatccac
     8641 gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc
     8701 aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc
     8761 aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc gctctagatc
     8821 tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca gaaaatgttc
     8881 aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag
     8941 atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag
     9001 atgtcatacg aagcgcagat gcacgttttc gagagattcc gcctatccca gcaagaaata
     9061 cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg
     9121 gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcta
     9181 aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat
     9241 ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc taacaacgga
     9301 acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact
     9361 aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga
     9421 accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga tttatatggg
     9481 agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg
     9541 tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa tgactgtagc
     9601 tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca agctgaattt
     9661 gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat atgtgatcgg
     9721 tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt gcaataaaca
     9781 agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc cagaattttt
     9841 gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga atatgatttc
     9901 tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg atgtcaaacc
     9961 aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga cagaagctga
    10021 ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc tggctatcgt
    10081 catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg aagcaaacgg
    10141 atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa ctgactttgg
    10201 aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta gaaagcattg
    10261 tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta aatgtctctg
    10321 aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt caagtcatca
    10381 tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca ggcgtgaaat
    10441 taggagaaaa actttcttct gtaagtgcag ctacagcaaa ttcatcagca tctggaaaag
    10501 accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa caaaaaccat
    10561 ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg attcctgcat
    10621 ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta gtcacagccg
    10681 gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg
    10741 ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt tttggtgcaa
    10801 caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg aatccggaag
    10861 caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt gggatcctag
    10921 gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac
    10981 ctgatgcggt agatatcatc gttacaccaa ctctttcatt gattgccatc ggtctgattg
    11041 aaattttctt catcatgcct ttagcaggtt tcatttctga tggcatggtc ggtgggatca
    11101 actgggtact tggcgttggt ggagcatttg ccggcttcgt tctaggcaca ttattcttgc
    11161 cgatggttat gttcggactg catcaaattt tgactccaat ccatgtgcaa atgattgatg
    11221 aaactggtcg tacgctattg cttccaattc ttgcgatggc tggtgcagga caagtgggtg
    11281 cagcattggc attatggatt cgctgcaaaa aagataaaga attaacagaa atgatcaaag
    11341 gagctttacc agtcgggatc ctaggaatcg gcgaaccatt gatttacgga gtaacattac
    11401 ctctaggacg cccatttatt actgcttgta tcggtggagg tatcggcggg gcagtgatcg
    11461 gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc aggagccgct ttgatcccat
    11521 tgatctctga cggtaaatgg ttaggctaca tccttggatt attggcagct tatgctggtg
    11581 gcttcgttgc tacattcttc ttcggtattc ctaaagaccg tttagaaaaa gaagaattag
    11641 cgactgaaac agccacagta aatgaagtgc ctgaaaaagt agtacctcaa acttcagctg
    11701 aaagtgtgac tatcctctca tcagttgctg acggaacagt tgtttcttta gataaagcaa
    11761 gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg
    11821 gacatatcta ttctccagtt actggggtag tcagctctgt cttcccaacg aaacacgcta
    11881 ttgggatcac aacgccaaat ggtttagaaa tcttgcttca tatgggaatc aacacagttg
    11941 acttaggcgg tacgccattt gatgtgaaag taacagaagg gcagcaagtg acacctgata
    12001 cgcttgtagc agaagctgac ttagaagcga tcaaagcagc aggcaaagaa acatccatga
    12061 tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag
    12121 caaaagcaaa agcacctgtg atggatgtca aagcttaata tgatagaaac aatagaaacg
    12181 aaagaagaaa ctgaaatcta tttttcagct tcttcttttt tattatttat tgttttctgc
    12241 taaaatctga taaattcgtg tcacatcaga tggtgttccc cgtaattcat aatctgccaa
    12301 gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt agacaatact gattattgaa
    12361 gtttttattt ccgctaccga ctacaccaag acaaagttta tgattgtctt catgttccag
    12421 atattcacgc atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac catttccgcc
    12481 gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt
    12541 ttcatggatt tcttttaaag taacttcagg attgacagga tcttctgcat gcattttttc
    12601 agcatattct gctagatgtt tggcaaaggc acgcgtattt cctgaaatcg atatatataa
    12661 aatgttcatg gatcatcctc caattctttc cttttagtat agcatctttt ttaagttttt
    12721 tggtggtgta atttggaaag ggactaggcg ctttgcttgt ataatgagaa aaaagccggt
    12781 agaattatat ttctattaaa gtttttatta attaagtaaa aataaaaaag gtttgttata
    12841 ctttttgata aaagcggttt tattttggag gatgcttaca tggatacaga gcaacagaaa
    12901 aaaggaattg gtttaattcc acttgctgcc ttagtaattg gttcagcgat tggaggtggc
    12961 gtatttggta ttatgacgga catctccgga tcggctggcg gcccagctat tttcagttgg
    13021 gtactcgtag gaacagcaat gttgatgctc tccttgtcga tcaataatat caataaaaaa
    13081 cgtccagatt taaaaggcgg gatctttagt tacgcagaag aaggttttgg ccgtttcgct
    13141 ggttttatca gcggttgggg ttactggctc acatgctggt taggcaacgt agcttttgca
    13201 acattgttga tgagcgcatt aggatatttc ttcccagtat ttgaaggtgg gcaaaatctg
    13261 gcatcgatcg tgatgagtac agtcttttta tggggatatg catggctagt gaatcgggga
    13321 gtcgaaaatg ctagtttcgt caatgctatc gtcacattct gtaaattagt gccattattt
    13381 ttatttattg tagtagctat cagcacgttt tcgatccaac aattcattga taacttttct
    13441 agcgacatta tattgaatgc gaatggacag ccgatcccgt tttccagtca agtattgagt
    13501 tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag gagcctctgt cgtaggttca
    13561 cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc tagggatatt cggcttgatt
    13621 attatttacg tcttggtttc catgcttcca tatgggacaa tgacgatgaa tcagctgcaa
    13681 acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa tgatggttgg taaatgggga
    13741 gcagtcgtga tcaacggcgg tttgatcatt tctttagttg gtgtttggct ctcatggacg
    13801 atcttaccga ttgaaacaat gggaaacatg gctgaagacg gacttttgcc taaagcatgg
    13861 ggaaaactca acaaaaatgg ggcaccaact tatgctatcg tattgacgac gttatgtacg
    13921 aatgtctttt tactttcatt attattcact gatagtgctt ataactttgc ttacacactt
    13981 ggtacagcag ctattttctt cacgtggctt ttcttaggat tgtaccaaat gaaactttct
    14041 tatcagcgga aagaatgggt ccaattttta gtagggcttt tagcaagtgc ctttcaagta
    14101 tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg ttcttgttct tttcttgcca
    14161 ggcatttatt tttacctcaa agcacgtaag gagcaaaacg agcctgtaca acatatccac
    14221 ttagataaga ttttacttat aggtgtgacg atggtcggag tgattgcaat ggttttattt
    14281 gccactggga tgcttcacgt atagaagtat aaaagaagta taaagagagg ttgtgagcct
    14341 gtcgtttatt caaaaacata ctaggaaagg agctgtgaca caaatgttgt cacagctcct
    14401 ttttcagaat aaacggtgtt taagagacag ccaatcggca ataaggctga acgtctcttt
    14461 cacagcctct tttttgatga gtcatcatac attttttcgc ttaaatacga gatatcctaa
    14521 tgctaagaac aaaacgatgt aagctaaatt accgaatagc atttgattag tagaaagatg
    14581 agtcaattgc ttcatgatct cttcgtttcc aatttggttt tgcagattca acatactgat
    14641 cggattccat ttgatccact cccatttttg gatgagtgca ataaggatac cagatatgat
    14701 tgacgaagca aaataaaaga cgattcctgc tgagatggat gcaccggaac tattgataaa
    14761 gcaagcaatc atcaacacta aacttaaaat cagccataaa ccgatataac tacccaaagt
    14821 ataagtgaac aagctttgga tcagtgtttg accagtagaa accttctccg tgaaggaaat
    14881 cgaagggaaa agaatcagtt tcatcaacac agtgacaata atagctaaaa ggtataaata
    14941 aacagaataa ataactaatg tgatccattt gcttactaat atttctcctc gagaatactt
    15001 tcgataaagg agatttttca atgtaccata ttgtgcttcc atcgaaatga tcgtgcttgc
    15061 agctgcaatc atgataaaga caatccaaga tgtggcacta aaaagctgag cagtcatttc
    15121 aactggatca atgatcgtcg tgtatttttt ggctagaagt gctgttccta tgagcaaaac
    15181 aactaataga acagataaaa cagcagttga ctttttcttg atcaatttaa ataattcttg
    15241 ttttacaagc aatgtcattt ttcttcttcc ccctttactg aatccagaat ttccaataga
    15301 gaagattcca aatcattcgt cagatgctgg acgtctgtca catggatatt ttgtgcagac
    15361 aatagctgga tcacggttcc taataattct tcgttttctt ttaaaagctt gattttatca
    15421 ggatcttcca aagtatatcc agcttctgtc aagatcgttc gagcagctag atcatttgtt
    15481 gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt cagccattgt acattgtttg
    15541 atgatctttc cttgattgat caggatcagc tcatcgacca ttttttcaag ctcgctgagt
    15601 aagtgactgg agatcaagaa agaggtgccc tgagtagcca agtcggtaat gatttttcgc
    15661 acatctcttg ttgcttgtgg atccaaaccg ttcatcggct catctaaaat aacgatagac
    15721 ggatggttca gtaaggcaag agcaattccc aatttttgtt tcattcccaa cgagtaggat
    15781 ttcgcttttt tgtgaatgta cttttccatc ttgagctgat tcacgatagt gtcgatagca
    15841 gatacatcgt tggtttcaga gaataacttc agatgatcat agcctgttaa gaatggatag
    15901 atacccgggt attcgatcaa tgctcctact tgttccaacc ctttatgact agagggagaa
    15961 atctcatttt caccaataca gattgttcca tctgagtaat ggatcagtcc taaaatagct
    16021 ttcatgatcg ttgtttttcc agcgccatta ggacctacta atccaacgat tttgccaggg
    16081 gataagctaa aactgacatc gctgagggct tgtttgtgac caaagttctt ggataattct
    16141 tggaccgaaa gacttttcaa aaatattcac tccttttcta tagatagtac aagcatacac
    16201 ctgatgagtg gagaagtcgc taggtcattg gacttatttc ttattttttc ataaaaaaat
    16261 tgaatattaa ataaaaaggc ttattttttc tgatcagtac ttattccaag tgtctagtgg
    16321 ccgctagtaa tcttgatgcc aatgatactg atgagtagca tccccacaaa gaaccaagtg
    16381 gaaagattca gcttatcatg aaacaagacc acaccaatta tgaccgaacc aactgcgcca
    16441 atccctgtcc agataggata agagagactc atcggcaaac ttttgattgc ctttgacaaa
    16501 aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag aataattgat tttagaaaaa
    16561 ccatcactcc atttcatcat cgttgaccag aatacttcca aaattcctgc tatgattaat
    16621 tccatccatg ccatacttac attcctcctt aactataaaa aaatacacaa aaagaggaaa
    16681 atcatccctt aaaagaccta atggaatgat catcctcttt atcctacccg gtggcagatc
    16741 gtatatcttt ttctgtagta tagcacacct atccagatat cggcaatctc atacttttta
    16801 gtaaagtgta ccaagcactg acggatatat cgttagcgga aaagaagatg aatgctttgc
    16861 aatttcatag aaagaagttg ttatttatta atcatttaaa aaaaacagta actagacctt
    16921 atttctatat attatcgggc aataaattaa aaaaagcttt accttacaat aagtaagata
    16981 atttttgtga taaaaaaata acgaaaaata tttaaaaata gtgtctattt cgtgagaaaa
    17041 caactatatt ttttatgtgt tttgctttat aattaaaaaa atattgtcat tttatggagg
    17101 aaaatatatg gaaaattatc ttagcctttt aggcgtcttg attgttattc tcgggtttgc
    17161 atttaaatta gactcgatat tgatcgtaat ggttgcttta gttgttacag ctttgactgg
    17221 cggtcttggc attcaaggaa tgttagaact tttaggtaca agttttgtaa acaatcgcgg
    17281 gatggcgatt tttatcatca ttatgttggc aacaggaacg cttgaaagaa atggtctgaa
    17341 agaatcagcg gcttccttga tcaagcgatt caagaaagta tctgctggta tgattgtaga
    17401 tatttatggg atctttcgaa tgatctttgc ggcttttaac gtgagttttg gtggagttgc
    17461 tggatttgtt cgtccaatca ttttaccgat ggcattgggg accatcgaat caaaagggtt
    17521 agaagtccat cctaagcatg aagaagaact aaaaggtatg gcttcagcta tggaaaatgt
    17581 ctgctggttc tttggccaag tactattcat cggaggagca ggggcgttgc tagtccaatc
    17641 aacactgaaa gatctaggat atgacgtaac attgggacag cttgctttag tggaagttcc
    17701 agtggctatc acggctttag ttgtggcaag tgtctacttc tatcttaaag aaaagaaatt
    17761 agcgaagaaa tattatccag gaaaggggca ataagatgga cttttttaca aatcctgata
    17821 ttttattagg cgataaatta ctggagatcc tttatatcgt gatgggactc gtattgattt
    17881 acactggagt aagaaattta acagataaaa ccaatcctca tcgttatggg tcagcctttt
    17941 tctggacggt cttaggggta gttattgcag gaggaagatg gcttccggca atcgtgaacg
    18001 gtggcttgat ctttgcgatg accataccag caattgctaa gaaagtcagc aaaggagaaa
    18061 gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga caaactagga atgaaaattt
    18121 ttattccagc tttgagtatc ggtgtctttg cgatcttgtt tgctctattt accaatttag
    18181 gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat gctaatcttg atgttctttt
    18241 ctcgagataa tcgaccaagt acatttttag atgatgcagc agatatgctg ggcacagtag
    18301 ggccgttaag catgttgcca atgcttttag cttctctagg tgcggtattt acaagcgctg
    18361 gagttgggac agtgatttcc aatgcagtag gtacgatcgt tccagaagga aatgtgaaca
    18421 tcggcatcat catttacgct cttggcatgg tgatatttac ggtaatcatg gggaatgcat
    18481 ttgcggccat caccgtgatg acggttggga tcggtgcacc attcgtattt agtcatggag
    18541 cgaatccagc gttaatagga atggttgcat tgacttgcgg tttttgtggg accctgctga
    18601 caccgatggc tgctaacttc aatatcgtgc ctgtagcgat gctggagatg aaagacaaat
    18661 acggagtgat caaaaaccag ttgtttattg cgttatttat gctagttttc caaattgctt
    18721 atatgattct atttaaataa tagaagagag gggttgtgac agaataattg tcgcaacctc
    18781 ttctaacatt gtagtggaac actatttttt tataagaata tattaagttt gatctgttat
    18841 gttatttaaa ctattgtttc ctttatttac gctgttcaag gaaaatggta tgataaaaga
    18901 taaccatatg ttaaatcaat cggacggagg aacaacagat gaaagtacta gtaacaggtt
    18961 ttgatccatt tggtggggac aaagtgaatc cagcctatga agcagtaaaa aaaatgccag
    19021 atgaaatcag cggtgctgaa atcatcaaag tggaagtacc gaccgtattc gaaaaaagca
    19081 gtcaagtagt caaagaagcc atccaacagc accaaccaga tgtcgtgatc tgtgtcggac
    19141 aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc aatcaatcta gcagaagcaa
    19201 gaatccctga taatgaagga aatcagccat tcgatacggc tttagaagaa aacggtccag
    19261 ccgcttactt tacctcattg ccaatcaaag caatgacaaa aaatgtacaa gatcatggtc
    19321 taccagcgta catctcttat acagcaggaa catttgtctg caatgatatc atgtatcgtt
    19381 tgcttcattt gatcgaaaca caattcccgg caattcgagg tgggttcatc cacgtgccat
    19441 tctcacccga tcaagtgatc gatcgtccag tgggtacgcc ttctatgtct ttagaagaca
    19501 ttgcttcttc tttgacttat gcagttgaag cagcagtaga aaataaagaa gatatttccg
    19561 gaaatgccgg aacaactcac taatttagca agaaacactg gctggttgct aacgttatgt
    19621 tagtagccag ctttttgtaa ttagaaagga aagaaacagc cgtggaagaa tggcaatccg
    19681 tctttgaaga atggtttcca aaagaaatca gcaaaagcta tcccatcaaa atttctaaac
    19741 aatacacaag cagtcaacgt tgggagatct atgcaaaatt aacgaaaaaa cagcgggaac
    19801 tagtcgataa gcaccgccga tatctaatca gctctcgttt tatggaagaa cattatttag
    19861 ctgcaacaga ttgggtgttt tctgacttca agatcaatcc gttttttcga acaaaacgaa
    19921 gccagcaaaa attgtattgt gagtgtggta gagaattaaa agtgcaatac atcgttaaat
    19981 cgccaaaaac cggaaagatc ctaaaactag gcatcaacca ctttgccgat cacctgcatg
    20041 tctctccgac agtggcagct tcgatccatc agggaatgac aaaagttgat ctggccttag
    20101 acgaattatt atggcttaag cagaagaata tcgattttcc tgaaggacta tggcaaaaat
    20161 actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc atatttaccg gatataaaat
    20221 tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc gatttacata gcggattatc
    20281 aagcattgga aaatgaaatc aagaaaataa gtgaacacat caatgggcag tcaaaaaagc
    20341 gacagataaa aaaagaacta tttgacgact ttgcagaaga attagtaaag gatgtagaag
    20401 aattcttgat caactatcgc gcatttttac gtaaagattg gcaatccatc gtatatgaag
    20461 aagtgccagt acatcctaat gcttattttg aaacatttat ttccgtgttg agaaagacaa
    20521 aacgtcaacg aacaccagaa gtcactgctc agatggaata ttttgcgaaa aaccaacgat
    20581 tcatccaacc aaaaatctat ctatttattt ggaagcaata ttgtcgctat ggttttacag
    20641 aagggttctt cgacagcatt cctcggatag tcagaaatgg ttttttaaaa gtattgcgta
    20701 aagaacgaga agctatacaa tctgctgata agaaagaccg tacagtctca aaagaaaaat
    20761 ggcaactagt agtgaaagat atccaatctg gaaatgtgca agagacaatc gacaaatgga
    20821 aaggaaaaca ttatcgtttt actgaagctc agaaacaagc gttggaatat tatcagaagt
    20881 tagaagaatc actgcgtttc aatgatgagg caagaaagta tctgaaggaa ttactataaa
    20941 aaatagaggc tgggacagaa gcgtttaact ccaagaaata agaa
//
LOCUS       DACCCR010000252        20858 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.051, whole genome shotgun sequence.
ACCESSION   DACCCR010000252 DACCCR010000000
VERSION     DACCCR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.051"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            267..1304
                     /locus_tag="IVK93_003007"
     CDS             267..1304
                     /locus_tag="IVK93_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6388200.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IVK93_003008"
     CDS             complement(1393..1716)
                     /locus_tag="IVK93_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6388201.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IVK93_003009"
     CDS             complement(1703..2410)
                     /locus_tag="IVK93_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP6388202.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IVK93_003010"
     CDS             2496..2837
                     /locus_tag="IVK93_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP6388203.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IVK93_003011"
     CDS             complement(3008..3856)
                     /locus_tag="IVK93_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388204.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IVK93_003012"
     CDS             4454..5317
                     /locus_tag="IVK93_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6388205.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IVK93_003013"
     CDS             5417..5899
                     /locus_tag="IVK93_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6388206.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IVK93_003014"
     CDS             complement(6162..6629)
                     /locus_tag="IVK93_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP6388207.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IVK93_003015"
     CDS             6752..7603
                     /locus_tag="IVK93_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6388208.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IVK93_003016"
     CDS             complement(7854..9812)
                     /locus_tag="IVK93_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6388209.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IVK93_003017"
     CDS             complement(9809..10681)
                     /locus_tag="IVK93_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6388210.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IVK93_003018"
     CDS             complement(11023..11895)
                     /locus_tag="IVK93_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6388211.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IVK93_003019"
     CDS             12023..12592
                     /locus_tag="IVK93_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP6388212.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IVK93_003020"
     CDS             12659..13396
                     /locus_tag="IVK93_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6388213.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IVK93_003021"
     CDS             13627..14895
                     /locus_tag="IVK93_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP6388214.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IVK93_003022"
     CDS             15382..15846
                     /locus_tag="IVK93_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6388215.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IVK93_003023"
     CDS             15830..16459
                     /locus_tag="IVK93_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6388216.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IVK93_003024"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IVK93_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6388217.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IVK93_003025"
     CDS             18338..18784
                     /locus_tag="IVK93_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6388218.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IVK93_003026"
     CDS             complement(18983..19498)
                     /locus_tag="IVK93_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6388219.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20816
                     /locus_tag="IVK93_003027"
                     /pseudo
     CDS             19615..20816
                     /locus_tag="IVK93_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6628 a         3703 c         4314 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt atggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtgta ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc
    19921 ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg
    19981 ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca
    20041 cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc
    20101 tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt
    20161 tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga
    20221 gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg
    20281 gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt
    20341 tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc
    20401 tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga
    20461 caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt
    20521 ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca
    20581 atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt
    20641 ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga
    20701 ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca
    20761 gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa
    20821 tcagaggttg tgaaaaagct gtcctgcatc aagtcata
//
LOCUS       DACCCR010000253         8062 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.112, whole genome shotgun sequence.
ACCESSION   DACCCR010000253 DACCCR010000000
VERSION     DACCCR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.112"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            128..1564
                     /locus_tag="IVK93_003028"
     CDS             128..1564
                     /locus_tag="IVK93_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6388220.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IVK93_003029"
     CDS             1753..4200
                     /locus_tag="IVK93_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP6388221.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IVK93_003030"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IVK93_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP6388222.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IVK93_003031"
     CDS             complement(5690..6793)
                     /locus_tag="IVK93_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6388223.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IVK93_003032"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IVK93_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6388224.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DACCCR010000254         7767 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.115, whole genome shotgun sequence.
ACCESSION   DACCCR010000254 DACCCR010000000
VERSION     DACCCR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.115"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..212
                     /locus_tag="IVK93_003033"
     CDS             <1..212
                     /locus_tag="IVK93_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6388225.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
     gene            387..683
                     /locus_tag="IVK93_003034"
     CDS             387..683
                     /locus_tag="IVK93_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6388226.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="IVK93_003035"
     CDS             690..2642
                     /locus_tag="IVK93_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP6388227.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="IVK93_003036"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="IVK93_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP6388228.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3441..4613
                     /locus_tag="IVK93_003037"
     CDS             3441..4613
                     /locus_tag="IVK93_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP6388229.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHV
                     EIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLG
                     YLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLE
                     QVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPA
                     RCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            4711..6084
                     /gene="tet(L)"
                     /locus_tag="IVK93_003038"
     CDS             4711..6084
                     /gene="tet(L)"
                     /locus_tag="IVK93_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP6388230.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            6648..7760
                     /locus_tag="IVK93_003039"
     CDS             6648..7760
                     /locus_tag="IVK93_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAP6388231.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKGFCCKVLNKE"
BASE COUNT         2652 a         1193 c         1482 g         2440 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 atggcttata ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg
     4261 tattggaaca agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aaatttatgc gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg
     4381 cgaacatcgt agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg
     4441 ctcggtgtat tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt
     4501 gtttaacaga gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc
     4561 gtccaaatag tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt
     4621 tatgttgtta tataaatatg gtttcttgtt aaataagatg aaatattttt taataaagat
     4681 ttgaattaaa gtgtaaagga ggaattaatt atgaagtgta atgaatgtaa cagggttaaa
     4741 tacaaccaaa ttttaatttg gctttgcgtt ttatcttttt ttagcgtatt aaatgaaatg
     4801 gttttgaacg tctcattacc tgatattgca aatgatttta ataaaccacc tgcgagtaca
     4861 aactgggtga acacagcctt tatgttaacc ttttccattg gaacagctgt atatggaaag
     4921 ctatctgatc aattaggcat caaaaggtta ctcctatttg gaattataat aaattgtttc
     4981 gggtcggtaa ttgggtttgt tggccattct ttcttttcct tacttattat ggctcgtttt
     5041 attcaagggg ctggtgcagc tgcatttcca gcactcgtaa tggttgtagt tgcgcgctat
     5101 attccaaagg aaaatagggg taaagcattt ggtcttattg gatcgatagt agccatggga
     5161 gaaggagtcg gtccagcgat tggtggaatg atagcccatt atattcattg gtcctatctt
     5221 ctactcattc ctatgataac aattatcact gttccgtttc ttatgaaatt attaaagaaa
     5281 gaagtaagga taaaaggtca ttttgatatc aaaggaatta tactaatgtc tgtaggcatt
     5341 gtatttttta tgttgtttac aacatcatat agcatttctt ttcttatcgt tagcgtgctg
     5401 tcattcctga tatttgtaaa acatatcagg aaagtaacag atccttttgt tgatcccgga
     5461 ttagggaaaa atataccttt tatgattgga gttctttgtg ggggaattat atttggaaca
     5521 gtagcagggt ttgtctctat ggttccttat atgatgaaag atgttcacca gctaagtact
     5581 gccgaaatcg gaagtgtaat tattttccct ggaacaatga gtgtcattat tttcggctac
     5641 attggtggga tacttgttga tagaagaggt cctttatacg tgttaaacat cggagttaca
     5701 tttctttctg ttagcttttt aactgcttcc tttcttttag aaacaacatc atggttcatg
     5761 acaattataa tcgtatttgt tttaggtggg ctttcgttca ccaaaacagt tatatcaaca
     5821 attgtttcaa gtagcttgaa acagcaggaa gctggtgctg gaatgagttt gcttaacttt
     5881 accagctttt tatcagaggg aacaggtatt gcaattgtag gtggtttatt atccataccc
     5941 ttacttgatc aaaggttgtt acctatggaa gttgatcagt caacttatct gtatagtaat
     6001 ttgttattac ttttttcagg aatcattgtc attagttggc tggttacctt gaatgtatat
     6061 aaacattctc aaagggattt ctaaatcgtt aagggatcaa ctttgggaga gagttcaaaa
     6121 ttgatccttt ttttataaca ggaattcaaa tctttttgtt ccattaaagg gcgcgattgc
     6181 tgaataaaag atacgagaga cctctcttgt atctttttta ttttgagtgg ttttgtccgt
     6241 tacactagaa aaccgaaaga caataaaaat tttattcttg ctgagtctgg ctttcggtaa
     6301 gctagacaaa acggacaaaa taaaaattgg caagggttta aaggtggaga ttttttgagt
     6361 gatcttctca aaaaatacta cctgtccctt gctgattttt aaacgagcac gagagcaaaa
     6421 cccccctttg ctgaggtggc agagggcagg tttttttgtt tcttttttct cgtaaaaaaa
     6481 agaaaggtct taaaggtttt atggttttgg tcggcactgc cgacagcctc gcagagcaca
     6541 cactttatga atataaagta tagtgtgtta tactttactt ggaagtggtt gccggaaaga
     6601 gcgaaaatgc ctcacatttg tgccacctaa aaaggagcga tttacatatg agttatgcag
     6661 tttgtagaat gcaaaaagtg aaatcagctg gactaaaagg catgcaattt cataatcaaa
     6721 gagagcgaaa aagtagaacg aatgatgata ttgaccatga gcgaacacgt gaaaattatg
     6781 atttgaaaaa tgataaaaat attgattaca acgaacgtgt caaagaaatt attgaatcac
     6841 aaaaaacagg tacaagaaaa acgaggaaag atgctgttct tgtaaatgag ttgctagtaa
     6901 catctgaccg agattttttt gagcaactgg atccaggaga acaaaaacga ttttttgagg
     6961 aaagttataa attattttcc gaacgatatg gcaagcaaaa tattgcttat gcaacagttc
     7021 ataatgatga gcaaacccct cacatgcatt taggtgttgt gcctatgcgt gatggaaaac
     7081 tgcaaggaaa aaatgtgttt aatcgtcaag aactgttatg gctacaagat aaattccccg
     7141 agcatatgaa aaaacagggt tttgagttga agcgtggtga acgtggctct gaccgtaaac
     7201 atattgagac agctaaattt aaaaaacaaa ctttggaaaa agagattgat tttctagaaa
     7261 aaaatttagc agttaaaaaa gatgaatgga ctgcttatag cgataaagtt aaatcagatt
     7321 tagaagtacc agcgaaacga cacatgaaaa gtgttgaagt gccaacgggt gaaaagtcca
     7381 tgtttggttt gggaaaagaa ataatgaaaa cagaaaagaa accaaccaaa aatgttgtta
     7441 tatcggagcg tgattataaa aacttagtga ctgctgcgag agataacgat aggttaaaac
     7501 agcatgttag aaatctcatg agtactgata tggcgagaga atataaaaaa ttaagtaaag
     7561 aacatgggca agttaaagaa aaatatagtg gtcttgtaga gcgatttaat gaaaatgtaa
     7621 atgattataa tgagttgctt gaagaaaaca agtctttaaa gtctaaaata agcgatttaa
     7681 agcgtgatgt gagtttaatc tatgaaagca ctaaggaatt ccttaagggg ttctgttgca
     7741 aagttttaaa taaagaataa aatccct
//
LOCUS       DACCCR010000255         7616 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.117, whole genome shotgun sequence.
ACCESSION   DACCCR010000255 DACCCR010000000
VERSION     DACCCR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.117"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            244..1212
                     /locus_tag="IVK93_003040"
     CDS             244..1212
                     /locus_tag="IVK93_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP6388232.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1205..2236
                     /gene="vanA"
                     /locus_tag="IVK93_003041"
     CDS             1205..2236
                     /gene="vanA"
                     /locus_tag="IVK93_003041"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP6388233.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2242..2850
                     /locus_tag="IVK93_003042"
     CDS             2242..2850
                     /locus_tag="IVK93_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP6388234.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3278..4189
                     /gene="vanY"
                     /locus_tag="IVK93_003043"
     CDS             3278..4189
                     /gene="vanY"
                     /locus_tag="IVK93_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP6388235.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4342..4827
                     /gene="vanZ"
                     /locus_tag="IVK93_003044"
     CDS             4342..4827
                     /gene="vanZ"
                     /locus_tag="IVK93_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP6388236.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5114..5443)
                     /locus_tag="IVK93_003045"
     CDS             complement(5114..5443)
                     /locus_tag="IVK93_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6388237.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5748..6077
                     /locus_tag="IVK93_003046"
     CDS             5748..6077
                     /locus_tag="IVK93_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6388238.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6067..6354
                     /locus_tag="IVK93_003047"
     CDS             6067..6354
                     /locus_tag="IVK93_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6388239.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(6633..7196)
                     /locus_tag="IVK93_003048"
     CDS             complement(6633..7196)
                     /locus_tag="IVK93_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6388240.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2499 a         1278 c         1519 g         2320 t
ORIGIN      
        1 tgctggtgtc atccaccaac atcaaaaagt atagagccat aattttttag gaaaatctca
       61 aggttatctt tactttttct taggaaatta acaatttaat attaagaaac ggctcgttct
      121 tacacggtag acttaatacc gtaagaacga gccgttttcg ttcttcagag aaagatttga
      181 caagattacc attggcatcc ccgttttatt tggtgccttt cacagaaagg gttggtctta
      241 attatgaata acatcggcat tactgtttat ggatgtgagc aggatgaggc agatgcattc
      301 catgctcttt cgcctcgctt tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa
      361 tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg tgggacataa atcagagatt
      421 tccgcctcta ttcttcttgc gctgaagaga gccggtgtga aatatatttc tacccgaagc
      481 atcggctgca atcatataga tacaactgct gctaagagaa tgggcatcac tgtcgacaat
      541 gtggcgtact cgccggatag cgttgccgat tatactatga tgctaattct tatggcagta
      601 cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac
      661 cgtggcaagg tactcagcga catgacagtt ggtgtggtgg gaacgggcca gataggcaaa
      721 gcggttattg agcggctgcg aggatttgga tgtaaagtgt tggcttatag tcgcagccga
      781 agtatagagg taaactatgt accgtttgat gagttgctgc aaaatagcga tatcgttacg
      841 cttcatgtgc cgctcaatac ggatacgcac tatattatca gccacgaaca aatacagaga
      901 atgaagcaag gagcatttct tatcaatact gggcgcggtc cacttgtaga tacctatgag
      961 ttggttaaag cattagaaaa cgggaaactg ggcggtgccg cattggatgt attggaagga
     1021 gaggaagagt ttttctactc tgattgcacc caaaaaccaa ttgataatca atttttactt
     1081 aaacttcaaa gaatgcctaa cgtgataatc acaccgcata cggcctatta taccgagcaa
     1141 gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt tggattttga aaggagacag
     1201 gagcatgaat agaataaaag ttgcaatact gtttgggggt tgctcagagg agcatgacgt
     1261 atcggtaaaa tctgcaatag agatagccgc taacattaat aaagaaaaat acgagccgtt
     1321 atacattgga attacgaaat ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg
     1381 ggaaaacgac aattgctatt cagctgtact ctcgccggat aaaaaaatgc acggattact
     1441 tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat gtagcatttt cagctttgca
     1501 tggcaagtca ggtgaagatg gatccataca aggtctgttt gaattgtccg gtatcccttt
     1561 tgtaggctgc gatattcaaa gctcagcaat ttgtatggac aaatcgttga catacatcgt
     1621 tgcgaaaaat gctgggatag ctactcccgc cttttgggtt attaataaag atgataggcc
     1681 ggtggcagct acgtttacct atcctgtttt tgttaagccg gcgcgttcag gctcatcctt
     1741 cggtgtgaaa aaagtcaata gcgcggacga attggactac gcaattgaat cggcaagaca
     1801 atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt
     1861 attgggaaac agtgccgcgt tagctgttgg cgaggtggac caaatcaggc tgcagtacgg
     1921 aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac
     1981 cgttcccgca gacctttcag cagaggagcg aggacggata caggaaacgg caaaaaaaat
     2041 atataaagcg ctcggctgta gaggtctagc ccgtgtggat atgtttttac aagataacgg
     2101 ccgcattgta ctgaacgaag tcaatactct gcccggtttc acgtcataca gtcgttatcc
     2161 ccgtatgatg gccgctgcag gtattgcact tcccgaactg attgaccgct tgatcgtatt
     2221 agcgttaaag gggtgataag catggaaata ggatttactt ttttagatga aatagtacac
     2281 ggtgttcgtt gggacgctaa atatgccact tgggataatt tcaccggaaa accggttgac
     2341 ggttatgaag taaatcgcat tgtagggaca tacgagttgg ctgaatcgct tttgaaggca
     2401 aaagaactgg ctgctaccca agggtacgga ttgcttctat gggacggtta ccgtcctaat
     2461 cgtgctgtaa actgttttat gcaatgggct gcacagccgg aaaataacct gacaaaggaa
     2521 agttattatc ccaatattga ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa
     2581 tcaagccata gccgcggcag tgccattgat cttacgcttt atcgattaga cacgggtgag
     2641 cttgtaccaa tggggagccg atttgatttt atggatgaac gctctcatca tgcggcaaat
     2701 ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt
     2761 gggtttgaag catatagcct cgaatggtgg cactatgtat taagagacga accatacccc
     2821 aatagctatt ttgatttccc cgttaaataa acttttaacc gttgcacgga caaactatat
     2881 aagctaactc tttcggcagg aaacccgacg tatgtaactg gttcttaggg aatttatata
     2941 tagtagatag tattgaagat gtaaggcaga gcgatattgc ggtcattatc tgcgtgcgct
     3001 gcggcaagat agcctgataa taagactgat cgcatagagg ggtggtattt cacaccgccc
     3061 attgtcaaca ggcagttcag cctcgttaaa ttcagcatgg gtatcactta tgaaaattca
     3121 tctacattgg tgataatagt aaatccagta gggcgaaata attgactgta atttacgggg
     3181 caaaacggca caatctcaaa cgagattgtg ccgtttaagg ggaagattct agaaatattt
     3241 catacttcca actatatagt taaggaggag actgaaaatg aagaagttgt tttttttatt
     3301 gttattgtta ttcttaatat acttaggtta tgactacgtt aatgaagcac tgttttctca
     3361 ggaaaaagtc gaatttcaaa attatgatca aaatcccaaa gaacatttag aaaatagtgg
     3421 gacttctgaa aatacccaag agaaaacaat tacagaagaa caggtttatc aaggaaatct
     3481 gctattaatc aatagtaaat atcctgttcg ccaagaaagt gtgaagtcag atatcgtgaa
     3541 tttatctaaa catgacgaat taataaatgg atacgggttg cttgatagta atatttatat
     3601 gtcaaaagaa atagcacaaa aattttcaga gatggtcaat gatgctgtaa agggtggcgt
     3661 tagtcatttt attattaata gtggctatcg agactttgat gagcaaagtg tgctttacca
     3721 agaaatgggg gctgagtatg ccttaccagc aggttatagt gagcataatt caggtttatc
     3781 actagatgta ggatcaagct tgacgaaaat ggaacgagcc cctgaaggaa agtggataga
     3841 agaaaatgct tggaaatacg ggttcatttt acgttatcca gaggacaaaa cagagttaac
     3901 aggaattcaa tatgaactat ggcatattcg ctatgttggt ttaccacata gtgcgattat
     3961 gaaagaaaag aatttcgttc tcgaggaata tatggattac ctaaaagaag aaaaaaccat
     4021 ttctgttagt gtaaatgggg aaaaatatga gatcttttat tatcctgtta ctaaaaatac
     4081 caccattcat gtgccgacta atcttcgtta tgagatatca ggaaacaata tagacggtgt
     4141 aattgtgaca gtgtttcccg gatcaacaca tactaattca aggaggtaag gatggcggaa
     4201 tgaaaccaac gaaattaatg aacagcatta ttgtactagc acttttgggg taacgttagc
     4261 tttttaattt aaaacccacg ttaactagga cattgctata ctaatgatac aacttaaaca
     4321 aaagaattag aggaaattat attgggaaaa atattatcta gaggattgct agctttatat
     4381 ttagtgacac taatctggtt agtgttattc aaattacaat acaatatttt atcagtattt
     4441 aattatcatc aaagaagtct taacttgact ccatttactg ctactgggaa tttcagagag
     4501 atgatagata atgttataat ctttattcca tttggcttgc ttttgaatgt caattttaaa
     4561 gaaatcggat ttttacctaa gtttgctttt gtactggttt taagtcttac ttttgaaata
     4621 attcaattta tcttcgctat tggagcgaca gacataacag atgtaattac aaatactgtt
     4681 ggaggctttc ttggactgaa attatatggt ttaagcaata agcatatgaa tcaaaaaaaa
     4741 ttagacagag ttattatttt tgtaggtata cttttgctcg tattattgct cgtttaccgt
     4801 acccatttaa gaataaatta cgtgtaagat gtctaaatca agcaatctga tctttcatac
     4861 acataaagat attgaatgaa ttggattaga tggaaaacgg gatgtgggga aactcgcccg
     4921 taggtgtgaa gtgaggggaa aaccggtgat aaagtaaaaa gcttacctaa cactatagta
     4981 acaaagaaag cccaattatc aattttagtg ctgaggaatt ggtctcttta ataaatttcc
     5041 ttaacgttgt aaatccgcat tttcctgacg gtacccccaa actaagattt catagataga
     5101 ttttctgata actttaaaaa acacgaatag cggaaaggat gtcttcattc gctaaagagt
     5161 aataaactac ttttccatct tgacgtgatt ttgccattcc atgttttttt aaaaaacgta
     5221 aatggtgtga cgttgtagcg acactagcag taagtatttc tgctaaatca cagacgcaca
     5281 tttccttata ggtttctaaa gcatagaaaa ttttaattct agaaaaatcg ctgaaacatt
     5341 tccctaacac aagtaagttt gaaaaatctt tttgatccaa tttatttttt attccattta
     5401 ctttttcttt atgtacgaca gtcacttggc aaactttttt cattcatctc acctcacatt
     5461 caaacgatta tttgaatgaa gtatacctat ttgatcactc ttttgtcaat ctaccttaat
     5521 tccaatacac tattataagc aatttcatgt attgaagtaa acttacttgc aagtgcgttt
     5581 ttgattccaa gttaaaaaca tattattatt ctacataatt tcctaatcaa ataaaactcc
     5641 tttgaaaatt gttctaaagg agttttatta ttttgtattt ctactaaaaa tagcgtacaa
     5701 tgtaattact aagtaattac gaaatgtaaa ataatggagg aaataatatg attacaaaaa
     5761 caagaaaaca aggaaattct attatgttaa cggtccctaa agaattcgat gtgcccaatg
     5821 gtgttgaagt agaagctaag cttgtagaga atggtatcct ttatgagttt gtagagccta
     5881 aaaaagaatt ttttgatttt agtgaagata ttttggctga tattctttca gaaggttata
     5941 gcaaacaaga gatcttaaaa gaatttagaa atagaaaaaa tgcgttaact tctgcattta
     6001 aatctattgc tgaagatact gttgcaaatt ctaaaccgat gacaaaagag gagttggcag
     6061 cagaaattgg cttatgagat tctaccttct aaacacgtaa taaagtatct aaaaaaatta
     6121 aaagagaaaa cattaaaaga acaattttta acgataattt atgatgagat agcagttcgt
     6181 cctcattcgg gagaacaaaa aacgggtgat ttatcaggaa tttgggctat tggattcaag
     6241 tatgcaggta cgacatatcg cgttgcatat gaaattaaag ataatgcagt gatacctatc
     6301 ttactatgtg gaacgcatga aaatttttat gaacaattaa aaaagattag gtaagaaccc
     6361 aatgtttcaa cttgagagga tagacgacac tttaaaagat aatttttaac agctgagagt
     6421 tgtaataccc tataatttta aaccactaac ctatatattt atgaaaaata gaaaaaatta
     6481 aaacgcattt taagcctctt tcaaattaaa agcttcatcc ttatgcacaa agagggctta
     6541 aaatgcgtta ttttatattg ttttttagta ataataaaaa aatcactcta attctcccat
     6601 aaatattata gattcctaaa gattaaaata gactattccc tattgtcttt taagtagcgg
     6661 tagagtgttg ccttgctaat accagtcatt tccgtaattt cttttacgct ataaacctga
     6721 cttttataca attttactgc tcgatcaaca tctttctggt tcactcgtgg acggccacct
     6781 ttacgacctc tcgcacgtgc acttttcaaa ccttctttgg tacgttcaac aattaaatca
     6841 cgttcgaact gactaaatgc ttggaagaca gtcatcatta agcgtccgtg aggcgtgctg
     6901 gtatcgaagt tttctttcaa gctcaccagc ttaacatggt gttcttcaaa ataggtaaca
     6961 aggtcaatca aatcctttgt actacgaccc aatcgagaaa aactttctac aattaccgta
     7021 tctcccggac gaattttatc ttttaaacga tttagttggg gacgaaccgc tttggttccc
     7081 gtcattttct cagttaggat ttcattacaa ttttgttcat tgagtaagtc taattgacga
     7141 tttagttctt gtgaacgtgt actcactcgg gcataaccat aaatgtatcc actcatgctt
     7201 ttcttccttt cgtactttaa ttctggcttg attgtatcat aaaagggagt tatagataca
     7261 tagttaatgc aacaagttat gatacaaaat atgacctctt ttatttaata aaagaggtca
     7321 tttcattttg tctcataatc gattgttttt gatacgagtg tgtatacgaa ataaacaaaa
     7381 aaatcttaag taaatttcta gatacctaat ttaatcctaa ctagtgtatc ctaaagttaa
     7441 taacgagttt atcacaatga aaattataaa atgctcccat attagatata acatgcaata
     7501 tttgaattag aactacaaaa tttattcgtt ttataaagta aataatgttg agatactaag
     7561 gaatagaata ttataagggg ttctgttgca aagttttaaa tctactatca aataag
//
LOCUS       DACCCR010000256         5095 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.135, whole genome shotgun sequence.
ACCESSION   DACCCR010000256 DACCCR010000000
VERSION     DACCCR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.135"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IVK93_003049"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IVK93_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6388241.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IVK93_003050"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IVK93_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP6388242.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3976..5088
                     /locus_tag="IVK93_003051"
     CDS             3976..5088
                     /locus_tag="IVK93_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAP6388243.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKGFCCKVLNKE"
BASE COUNT         1710 a          751 c         1056 g         1578 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga
     4021 ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt
     4081 gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac
     4141 gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat
     4201 gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat
     4261 ccaggagaac aaaaacgatt ttttgaggaa agttataaat tattttccga acgatatggc
     4321 aagcaaaata ttgcttatgc aacagttcat aatgatgagc aaacccctca catgcattta
     4381 ggtgttgtgc ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa tcgtcaagaa
     4441 ctgttatggc tacaagataa attccccgag catatgaaaa aacagggttt tgagttgaag
     4501 cgtggtgaac gtggctctga ccgtaaacat attgagacag ctaaatttaa aaaacaaact
     4561 ttggaaaaag agattgattt tctagaaaaa aatttagcag ttaaaaaaga tgaatggact
     4621 gcttatagcg ataaagttaa atcagattta gaagtaccag cgaaacgaca catgaaaagt
     4681 gttgaagtgc caacgggtga aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca
     4741 gaaaagaaac caaccaaaaa tgttgttata tcggagcgtg attataaaaa cttagtgact
     4801 gctgcgagag ataacgatag gttaaaacag catgttagaa atctcatgag tactgatatg
     4861 gcgagagaat ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa atatagtggt
     4921 cttgtagagc gatttaatga aaatgtaaat gattataatg agttgcttga agaaaacaag
     4981 tctttaaagt ctaaaataag cgatttaaag cgtgatgtga gtttaatcta tgaaagcact
     5041 aaggaattcc ttaaggggtt ctgttgcaaa gttttaaata aagaataaaa tccct
//
LOCUS       DACCCR010000257         5095 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.136, whole genome shotgun sequence.
ACCESSION   DACCCR010000257 DACCCR010000000
VERSION     DACCCR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.136"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IVK93_003052"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IVK93_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6388244.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IVK93_003053"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IVK93_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP6388245.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3976..5088
                     /locus_tag="IVK93_003054"
     CDS             3976..5088
                     /locus_tag="IVK93_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAP6388246.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKGFCCKVLNKE"
BASE COUNT         1709 a          751 c         1057 g         1578 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga
     4021 ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt
     4081 gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac
     4141 gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat
     4201 gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat
     4261 ccaggagaac aaaaacgatt ttttgaggaa agttataaat tattttccga acgatatggc
     4321 aagcaaaata ttgcttatgc aacagttcat aatgatgagc aaacccctca catgcattta
     4381 ggtgttgtgc ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa tcgtcaagaa
     4441 ctgttatggc tacaagataa attccccgag catatgaaaa aacagggttt tgagttgaag
     4501 cgtggtgaac gtggctctga ccgtaaacat attgagacag ctaaatttaa aaaacaaact
     4561 ttggaaaaag agattgattt tctagaaaaa aatttagcag ttaaaaaaga tgaatggact
     4621 gcttatagcg ataaagttaa atcagattta gaagtaccag cgaaacgaca catgaaaagt
     4681 gttgaagtgc caacgggtga aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca
     4741 gaaaagaaac caaccaaaaa tgttgttata tcggagcgtg attataaaaa cttagtgact
     4801 gctgcgagag ataacgatag gttaaaacag catgttagaa atctcatgag tactgatatg
     4861 gcgagagaat ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa atatagtggt
     4921 cttgtagagc gatttaatga aaatgtaaat gattataatg agttgcttga agaaaacaag
     4981 tctttaaagt ctaaaataag cgatttaaag cgtgatgtga gtttaatcta tgaaagcact
     5041 aaggaattcc ttaaggggtt ctgttgcaaa gttttaaata aagaataaaa tccct
//
LOCUS       DACCCR010000258         4995 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.137, whole genome shotgun sequence.
ACCESSION   DACCCR010000258 DACCCR010000000
VERSION     DACCCR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.137"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(30..2180)
                     /locus_tag="IVK93_003055"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="IVK93_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="IVK93_003056"
     CDS             2326..2901
                     /locus_tag="IVK93_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6388247.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="IVK93_003057"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="IVK93_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP6388248.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="IVK93_003058"
     CDS             3788..4942
                     /locus_tag="IVK93_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6388249.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DACCCR010000259         4087 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.146, whole genome shotgun sequence.
ACCESSION   DACCCR010000259 DACCCR010000000
VERSION     DACCCR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.146"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            165..830
                     /locus_tag="IVK93_003059"
                     /pseudo
     CDS             165..830
                     /locus_tag="IVK93_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            866..1111
                     /locus_tag="IVK93_003060"
     CDS             866..1111
                     /locus_tag="IVK93_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6388250.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1214..2083
                     /locus_tag="IVK93_003061"
     CDS             1214..2083
                     /locus_tag="IVK93_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP6388251.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2064..2798
                     /locus_tag="IVK93_003062"
     CDS             2064..2798
                     /locus_tag="IVK93_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6388252.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2831..3739
                     /locus_tag="IVK93_003063"
     CDS             2831..3739
                     /locus_tag="IVK93_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP6388253.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3736..4047
                     /locus_tag="IVK93_003064"
                     /pseudo
     CDS             3736..4047
                     /locus_tag="IVK93_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1431 a          583 c          862 g         1211 t
ORIGIN      
        1 aattctgatt catcagtccc ataaattata gagccaaaaa tagaggtctt tccatttaga
       61 tagtagtgga aagacctcta tttatctttt gaaagtgaat acaaaaataa agcacactct
      121 gctataatat aggtgattaa tccatttgag agaggtgctt tatcatgtat caaaattata
      181 ccataaacca actttgtttg cctatagatc ttgaaattaa attagaagaa aatgattttg
      241 ctcatgccat tgtgcagttt gttgactcta ttcccgatga agtctttctt ccttattatc
      301 aatctatggg tagaccccaa tatcatcctc gtatgctact ttccattatt ctttgtgcct
      361 atattcaagg ggtttattcg ggccgccaaa tacaaaatat gctgatagat agtattcgga
      421 tgcgctactt atctcaagag caattcccta actttcgtac tattaatcgt tttcgcgttc
      481 atccgattat gaacgatatt ctagatcatt catttgttca atttcgtgag ctactggttc
      541 aaagtggctt aatcagtggc agtgctctat acattgatgg tacaaaaatt gaagcggatg
      601 cgaataaata ctctttcgtt tggaaaaaaa gtattttaaa gtataaggat agccttgatc
      661 aaaaagccct tgaaaactac caacaaatgg tggaaacaaa aatcttacct gagttaatag
      721 atgatttaaa ggatgaactt tctatagaag agattgaaag gattcgtcaa tctttggaag
      781 aaaaggaaaa tcaattaatt gaagcgattg accaaaccga aacagtagaa gggggctgac
      841 ccattattaa taaggagtgg acaacatgat tgatgtaaat gagctattgg aagaagctat
      901 tagagaaact gaaaatctaa atgatggcga agtttttctt gttaaagatt tattcaaggg
      961 atatgtgggg aataggatac ccagaaagga tcgacttctg cttggaacgt tatttttaaa
     1021 ccatgttagc aaaatgaatg gtaatttaaa ggctattgaa aagacttcat ctaatcagca
     1081 gaggtataaa aagacaattg ataagtttta actatctttt gtgaaattaa aaaaattata
     1141 aatatgttga gtagaagcaa aagatttggc ttggaatttt tacagcagtg aatcgataaa
     1201 aggagcatgc ttgatggtag ataatattat taaatcagta gcagagaaat tatcctctct
     1261 gtcttatata gaaggtattg ttttaggtgg ttcacgtgca aggggcaccc atacagagga
     1321 ttcggatata gatatcggca tctattacaa ttcagaatca tttgacataa atactattaa
     1381 tcaattcgct acaaagctgg atgatgagca tagaaataac cttgttgtac ctcccggagc
     1441 atggggtgat tggattaatg gcggcggatg gttagtcata aacgggtatc atgtggattt
     1501 aattttacgt gatattaaac gtgtggaaca aataatgaaa gatacagagc acggaattgt
     1561 tactgccaat tatcagactg ggcatcccca tggttatatt agtgcaatgt atcgaggaga
     1621 attagcgatt agcaaaatac tatatgctaa gaatgaaagc ttatgcgaat taaaaaaaca
     1681 ggcagaaact tatcccaatg ctttgcagaa aagtttagtt aactttttta tgtttgaagc
     1741 agggttctct ttaatgtttg taaaagcaaa ttcgggaaca gacgataaat attatattgc
     1801 gggtcatgtt tttcgtatag tttcatgttt aaatcaagtg ttatttgcat gtaataatgc
     1861 ttattgtatc aacgaaaaga aagctataaa actgcttgaa acttttgaac ataaacctga
     1921 aaaatatacc gagaaggtaa atcatatttt tgaagtactc ggtatctcac tttttgaatg
     1981 ctacgacatg accgagaagc tttataatga agtgaatgaa attgtatcgg agataaataa
     2041 ctttttaaac gaggagagtt cagatgaaag aaaacaaata tgatgataat atattttttc
     2101 aaaaatacag tcaaatgagt cgctcgcaga aaggactggc tggtgcggga gaatgggaga
     2161 ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt gcttgattta ggatgcggct
     2221 atggatggca ctgtatatat gcgatggaaa acggtgcttc ctctgtagta ggtgttgata
     2281 tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca ttttccacag attgaatatg
     2341 aatgctgtgc catagaagat gtggatttcc cagaggagag ctttgatgta atactaagtt
     2401 cgcttgcgtt tcattatgta gcagactatg agaatttaat aaaaaagata tataggatgc
     2461 tgaaggctgg tggcaattta gtttttacag ttgaacatcc tgtttttact gctcatggaa
     2521 cacaagactg gtattataac gaaaaaggag aaatactgca tttcccggtg gacaattatt
     2581 attatgaggg caaacggaca gctatgtttt tggaagaaaa ggttacaaaa tatcatagaa
     2641 cactgaccac atatctaaat acactgcttt caaatagttt tataataaat cagattgtgg
     2701 agccacagcc gccagagaac atgatggata ttccggggat ggcggatgaa atgcgacgcc
     2761 caatgatgct gattgtatcg gcaaaaaaga agatgtaata atatagaaaa aataaacgag
     2821 gagtatgtaa atgagatcag aaaaagaaat gatggattta gtactttctt tagcagaaca
     2881 ggatgaacgt attcgaattg tgacccttga ggggtcacgc gcaaatatta atatacctaa
     2941 agatgaattt caggattatg atattacata ttttgtaagt gatatagaac cgtttatatc
     3001 taatgatgac tggcttaatc aatttgggaa tataataatg atgcaaaagc cggaggatat
     3061 ggaattattc ccacctgaag aaaagggatt ttcctatctt atgctatttg atgattacaa
     3121 taaaattgat cttaccttat tgcccttgga agagttagat aattacctaa agggcgataa
     3181 attaataaag gttctaattg ataaagattg tagaattaaa agggacatag ttccgactga
     3241 tatagattat catgtaagaa agccaagcgc aagggagtat gatgattgct gcaatgaatt
     3301 ttggaatgta acaccttatg ttattaaagg attgtgccgt aaggaaattt tatttgctat
     3361 tgatcatttt aatcagattg ttcgccatga gctgctgaga atgatatcat ggaaggtcgg
     3421 catcgaaaca ggctttaaat taagtgtagg caagaactat aagtttattg aaaggtatat
     3481 atccgaggat ttgtgggaga aacttttgtc cacctaccgg atggattcct atgaaaacat
     3541 atgggaagca ttatttctat gccatcaatt gttcagggcg gtatccggtg aggtggcgga
     3601 aaggcttcat tatgcctatc cggagtatga taggaatata acaaaatata ccagggacat
     3661 gtataaaaaa tacactggta aaaccggctg cctggatagc acatatgccg ctgatataga
     3721 agagaggcgg gaacagtgat tacagaaatg aaagcagggc acctgaaaga tatcgataaa
     3781 cccagcgaac catttgaggt gataggtaag attataccga ggtatgaaaa cgagaattgg
     3841 acctttacag aattactcta tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat
     3901 gaagaggatg aggaggcaga ttgccttgaa tatattgaca atactgataa gataatatat
     3961 ctttactacc aagacgataa atgcgtcgga aaagttaaac tgcgaaaaaa ttggaaccgg
     4021 tacgcttata tagaagatat cgccgtatgg gttctgttgc aaagttttaa ataaagaata
     4081 aaatccc
//
LOCUS       DACCCR010000260         1998 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.170, whole genome shotgun sequence.
ACCESSION   DACCCR010000260 DACCCR010000000
VERSION     DACCCR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.170"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            47..439
                     /locus_tag="IVK93_003065"
     CDS             47..439
                     /locus_tag="IVK93_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6388254.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="IVK93_003066"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="IVK93_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP6388255.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          831 a          153 c          343 g          671 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaagtccag actcctgtgt
     1981 aaaatgctat acaatgtt
//
LOCUS       DACCCR010000261         1561 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG23-12-S
            SAMN13750727-rid10751943.guided.183, whole genome shotgun sequence.
ACCESSION   DACCCR010000261 DACCCR010000000
VERSION     DACCCR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750727
            Sequence Read Archive: SRR10270767
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860343.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860343.1
            Genome Coverage       :: 248x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 17:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750727-rid10751943.guided.183"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG23-12-S"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            107..190
                     /locus_tag="IVK93_003067"
     CDS             107..190
                     /locus_tag="IVK93_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP6388256.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            315..1052
                     /gene="erm(B)"
                     /locus_tag="IVK93_003068"
     CDS             315..1052
                     /gene="erm(B)"
                     /locus_tag="IVK93_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6388257.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1222..1482
                     /locus_tag="IVK93_003069"
                     /pseudo
     CDS             1222..1482
                     /locus_tag="IVK93_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
BASE COUNT          591 a          245 c          267 g          458 t
ORIGIN      
        1 atttgatagt agatttaaaa ctttgcaaca gaaccatttt gtgtataata ggaattgaag
       61 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
      121 aatgcgtaat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
      181 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
      241 gataactaaa attacaaaca aatcgtttaa cttctgtatt tatttacaga tgtaataact
      301 tcaggagtga ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
      361 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
      421 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
      481 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaacattc
      541 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
      601 aaattgttgg gagtattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
      661 aaagccatgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
      721 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
      781 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
      841 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
      901 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
      961 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta
     1021 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttt taaatttgga
     1081 aagttacacg ttactaaagg gaatggagat aaattattag atatactact gacagcttcc
     1141 aagaagctaa agaggtccct agcgcctacg gggaatttgt atcgataagg ggtacaaatt
     1201 cccactaagc gctcgggacc ccttgtagga aaatgtccta agtgtggcaa caatattgta
     1261 ttaaaaaaat cgttttatgg ttgttcaaat tatcctgaat gtaagtttac tttagctgaa
     1321 cattttagaa agaaaaaact aaccaaaacg aatgtaaaag aattactgga gggaaaagaa
     1381 accctggtaa aaggaatcaa aaacaaagag aaaaagccct acaatgccgt tgtaaaaatt
     1441 ggggaaaagg gatatattga ttttatctct ttttcaaaat aaacatgggg ctgacccaaa
     1501 agtagctcct atatgaaaaa tagggctctt tgtcaataag gactgatgag ttgtgcaaaa
     1561 t
//
